Rw3G018880

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000112
Physical Location & Seq
Forward (+)
21176367 .. 21179586
3220 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G018880.1

Sequence Viewer

Length: 2379 bp
ATGCTGTTGAAATCTCTGCTTCTCTTCCTCCTCTTCCAAGTTTGCACTTCCAGGGACACCTTGAGATCGAACGAAGAACTCAAGGATGATGATGGTGGACTTCTGGTGTCCAAAGAAAGCAGCTTTGAGTTGGGTTTCTTCAGCCCTGGAAATTCTAGTAACCGCTATGTTGGGATTTGGTATTCTCAATCTAAAGTATCTGAAAAAACAGTGGTTTTCACCATAAACAGGTATGGAGACCTAGTTCTTTATGCTTATAACATGGAGACCAGTCCTATTTGGTCTGCAGATGCGCCTTATTCAGTCAAAAACACTGGCACCTTATCTGCATATGTACAGCTGTTGGATACAGGAAACTTAGTTATGTTCCAGAATGATAGAGATGGAATCTTTGTATGGCAAAGTTTTGATTATCCTACAGATACTTTAATCCCTGGTATGAAAGTTGGGATGAATCGGAAAACTGGGCTAGAATGGGTTTTAACATCTTGGAAGTCAATAGATGGCCCTGGAACTGGGGACTATACCTTTAGGCTTCATAATTGGAGCCACGTTGTCACACCCCAGTATTTTATTTATAAAGGTTTGTCTAAGTATTGGCGAGGTGATACAGAGCCTTGGTCTAATTCGATCCGAAATGCAGAGGAAATGTCTTTTTTCCTTACCTATAACACCAATAAACTTACAATAATGAAGTTGATGTTTTCTGGGCTACATCAGCACTTGGTATGGAGTGATGCTGACCATCAATGGAAGGAGTTGTGGTCTTCACCGAAATACAGGTGTGACTGGTATGGACATTGTGGTGCCAACAGCAAATGTAGCCTTGACAGTGGTAATCTGTTCGAGTGTGAGTGTCTACCGGGGTATGAACCAAAATCTATAAATGGTTGGAATGGGAGAGATGGGTCAGATGGATGTGTGAGTAAACGAGTCAAAGTGTCAAAGTGTAGAAATGGAGAAGGGTTTGTGAAGGTGGAAAGGGTGAAAGATCCAGACGCATCGAAAGCAGCATTGTTGAAAAGAGGTATGAGTACCAAAGAGTGTGAGCAGGAGTACTGCCTAAGAAATTGTTCTTGCACGGCATATATGACCAAAAAGATTGAAGGGGTTGTTAATTGCTTGACATGGTATGATGACTTGATGGACATTTTGGTTTACACAGAGGCCGGACGAGATCTGTATGTTCGTGTGGATAAAATTGAGTTAGCTGAGAATGCCGCAAACTCACAAGGATTCTTCAGGAGGGGCATGCTTGTTGTTCCAATACTGTTTGCTCTGCTGGCCCTAGTACTAATAAATGTGATTGCCTGTTGGTTGCTTAAGAAAAAAGGGAAGGCAAAAGTCTTTGTGGAGGTGGATGAACTTGAGGAAAGCAAGAGATACCCGGAATTGCAATTTTTTGATCTGAACACACTAGTAGCAGCCACAGATAATTTCTCTGGTGTTAATAAACTTGGCGAAGGTGGTTTTGGTGGTGTTTATAAGGGTGAGTTACCAAATGGACAGAAGGTTGCTGTGAAAAGGCTGTCTAGCAGCTCAGGACAAGGGGTTGGTGAATTCAAAAATGAAGTTGAACTGATAGCAGGACTTCAACACAGGAATCTTGTGAAGCTTTTAGGTTGCTGTATAAAGGGAGGAGAAAGGATGCTAGTCCTCGAATATATGCCGAACAAAAGCTTGGACTCATTTCTTTTTGATAACACAAGAAAGTCCTTAGATTGGGGAAGGCGTTTTGAAATTATCAATGGGGTTGCTCGTGGGCTTTTGTATCTTCACCAAGACTCGAGATTAAGGATTATTCATAGGGATCTAAAAACCAGCAATATTCTTCTACATGATGAGATGACCCCAAAAATTTCAGATTTTGGCATGGCAAGAATATTCCATAGGGACCAACTTCACGATAACACAAACAAAGTTGTTGGAACATTTGGCTACATGTCACCAGAGTATGCAGTGTTTGGAAGATTCTCTACGAAATCGGATGTGTTCAGTTTTGGGATTGTACTATTGGAGATTGTTAGTGGCAAGAGAAACAATGGTTCTTATGAGGAGAATTACTCCATGAACCTGATAGGACATGTTTGGCATGTGTGGAAAGAAGGAATAGCCTTAGATATTGTGGATTCATCGCTACAATCATATAAGCATGATGAAGTCTTGAGATGCATTCAAATTGGACTGTTGTGCGTGCAAGAAGATTCCAAGGACCGGCCAGCCATGTCATCCGTTGTGTTCATGTTGAGTGGTGAGGCATCTCTTCCATCTCCTAAGCAGCCTGCATTTGTCTTCAGAAGAAATTCCAGCAGTGCTGCTGATTCAAAAGAATTGCATTCTATTAACGACTTAACAATAACTATTGTGGGAGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

792

Amino Acids

89.54

Weight (kDa)

7.2

Isoelectric Point (pI)

38.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 65 - 165 1.3e-24 D-mannose binding lectin
S_locus_glycop PF00954 214 - 294 5.1e-15 S-locus glycoprotein domain
PAN_2 PF08276 317 - 384 3.1e-15 PAN-like domain
Pkinase PF00069 483 - 744 2e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 484 - 746 4.6e-49 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 752 - 792 5.9e-09 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 258, 579, 1485
AccB1I GGYRCC 2 cut(s) 317, 806
AccI GTMKAC 1 cut(s) 859
AciI CCGC 2 cut(s) 163, 1221
AclWI GGATC 3 cut(s) 625, 986, 1818
AcoI YGGCCR 1 cut(s) 2216
AcsI RAATTY 4 cut(s) 151, 1559, 1857, 2302
AcuI CTGAAG 3 cut(s) 124, 1225, 2278
AfaI GTAC 5 cut(s) 336, 1036, 1058, 1293, 2010
AfiI CCNNNNNNNGG 4 cut(s) 228, 515, 1722, 2214
AflII CTTAAG 1 cut(s) 1322
AflIII ACRYGT 2 cut(s) 1941, 2083
AgsI TTSAA 9 cut(s) 10, 1021, 1106, 1564, 1577, 1595, 1739, 2177, 2325
AhlI ACTAGT 1 cut(s) 1417
AjnI CCWGG 4 cut(s) 50, 145, 433, 508
AjuI GAANNNNNNNTTGG 4 cut(s) 1455, 1487, 1664, 1696
AloI GAACNNNNNNTCC 2 cut(s) 228, 260
AluBI AGCT 7 cut(s) 123, 340, 1211, 1539, 1615, 1680, 2372
AluI AGCT 7 cut(s) 123, 340, 1211, 1539, 1615, 1680, 2372
Alw21I GWGCWC 1 cut(s) 2374
Alw26I GTCTC 2 cut(s) 231, 260
AlwI GGATC 3 cut(s) 625, 986, 1818
Ama87I CYCGRG 1 cut(s) 1786
AoxI GGCC 4 cut(s) 505, 1167, 1284, 2216
ApeKI GCWGC 6 cut(s) 120, 1010, 1424, 1536, 2278, 2315
ApoI RAATTY 4 cut(s) 151, 1559, 1857, 2302
Asp700I GAANNNNTTC 2 cut(s) 1072, 2302
AspLEI GCGC 1 cut(s) 295
AspS9I GGNCC 4 cut(s) 506, 1285, 1894, 2212
AsuC2I CCSGG 2 cut(s) 864, 1388
AsuHPI GGTGA 9 cut(s) 211, 617, 762, 997, 1502, 1568, 1769, 1938, 2264
AvaI CYCGRG 1 cut(s) 1786
AvaII GGWCC 2 cut(s) 1894, 2212
BanI GGYRCC 2 cut(s) 317, 806
BanII GRGCYC 1 cut(s) 2374
BarI GAAGNNNNNNTAC 2 cut(s) 1960, 1992
BauI CACGAG 1 cut(s) 1758
BbsI GAAGAC 2 cut(s) 759, 2284
Bbv12I GWGCWC 1 cut(s) 2374
BbvI GCAGC 6 cut(s) 132, 1022, 1436, 1548, 2290, 2302
BccI CCATC 8 cut(s) 86, 377, 497, 753, 899, 908, 1138, 2275
BceAI ACGGC 1 cut(s) 1098
BciT130I CCWGG 4 cut(s) 52, 147, 435, 510
BciVI GTATCC 1 cut(s) 340
BcnI CCSGG 2 cut(s) 864, 1388
BcoDI GTCTC 2 cut(s) 231, 260
BcuI ACTAGT 1 cut(s) 1417
BfaI CTAG 7 cut(s) 156, 242, 470, 1289, 1418, 1533, 1652
BfmI CTRYAG 2 cut(s) 285, 417
BfrI CTTAAG 1 cut(s) 1322
BfuI GTATCC 1 cut(s) 340
BglII AGATCT 1 cut(s) 1177
BisI GCNGC 7 cut(s) 121, 1011, 1221, 1425, 1537, 2279, 2316
BlsI GCNGC 7 cut(s) 122, 1012, 1222, 1426, 1538, 2280, 2317
BmcAI AGTACT 2 cut(s) 1058, 1293
Bme1390I CCNGG 6 cut(s) 52, 147, 435, 510, 864, 1388
Bme18I GGWCC 2 cut(s) 1894, 2212
BmeT110I CYCGRG 1 cut(s) 1786
BmgT120I GGNCC 4 cut(s) 506, 1285, 1894, 2212
BmiI GGNNCC 4 cut(s) 319, 548, 808, 1895
BmrFI CCNGG 6 cut(s) 52, 147, 435, 510, 864, 1388
BmrI ACTGGG 3 cut(s) 474, 525, 559
BmsI GCATC 6 cut(s) 280, 727, 1010, 1638, 2159, 2267
BmuI ACTGGG 3 cut(s) 474, 525, 559
BpiI GAAGAC 2 cut(s) 759, 2284
BplI GAGNNNNNCTC 2 cut(s) 2048, 2080
Bpu10I CCTNAGC 2 cut(s) 1540, 2274
BpuEI CTTGAG 4 cut(s) 65, 82, 1388, 2185
BpuMI CCSGG 2 cut(s) 864, 1388
BsaI GGTCTC 2 cut(s) 231, 260
BsaJI CCNNGG 7 cut(s) 51, 145, 433, 508, 617, 863, 2208
BsaXI ACNNNNNCTCC 4 cut(s) 228, 258, 749, 779
Bsc4I CCNNNNNNNGG 4 cut(s) 228, 515, 1722, 2214
Bse118I RCCGGY 1 cut(s) 2214
Bse1I ACTGG 6 cut(s) 270, 319, 469, 520, 565, 794
BseBI CCWGG 4 cut(s) 52, 147, 435, 510
BseDI CCNNGG 7 cut(s) 51, 145, 433, 508, 617, 863, 2208
BseGI GGATG 7 cut(s) 91, 456, 923, 1366, 1653, 1993, 2228
BseLI CCNNNNNNNGG 4 cut(s) 228, 515, 1722, 2214
BseMII CTCAG 2 cut(s) 1203, 1554
BseNI ACTGG 6 cut(s) 270, 319, 469, 520, 565, 794
BseRI GAGGAG 3 cut(s) 20, 1653, 2069
BseXI GCAGC 6 cut(s) 132, 1022, 1436, 1548, 2290, 2302
BshFI GGCC 4 cut(s) 507, 1169, 1286, 2218
BshNI GGYRCC 2 cut(s) 317, 806
BsiHKAI GWGCWC 1 cut(s) 2374
BsiHKCI CYCGRG 1 cut(s) 1786
BsiSI CCGG 4 cut(s) 863, 1170, 1388, 2215
BslFI GGGAC 3 cut(s) 68, 533, 1907
BslI CCNNNNNNNGG 4 cut(s) 228, 515, 1722, 2214
BsmAI GTCTC 2 cut(s) 231, 260
BsmFI GGGAC 3 cut(s) 68, 533, 1907
BsmI GAATGC 3 cut(s) 1222, 2172, 2335
BsnI GGCC 4 cut(s) 507, 1169, 1286, 2218
Bso31I GGTCTC 2 cut(s) 231, 260
BsoBI CYCGRG 1 cut(s) 1786
Bsp1286I GDGCHC 1 cut(s) 2374
Bsp1407I TGTACA 1 cut(s) 334
Bsp143I GATC 6 cut(s) 65, 630, 991, 1177, 1405, 1810
BspACI CCGC 2 cut(s) 163, 1221
BspANI GGCC 4 cut(s) 507, 1169, 1286, 2218
BspCNI CTCAG 2 cut(s) 1204, 1553
BspLI GGNNCC 4 cut(s) 319, 548, 808, 1895
BspMAI CTGCAG 1 cut(s) 289
BspPI GGATC 3 cut(s) 625, 986, 1818
BspT107I GGYRCC 2 cut(s) 317, 806
BspTI CTTAAG 1 cut(s) 1322
BspTNI GGTCTC 2 cut(s) 231, 260
BsrFI RCCGGY 1 cut(s) 2214
BsrGI TGTACA 1 cut(s) 334
BsrI ACTGG 6 cut(s) 270, 319, 469, 520, 565, 794
BssAI RCCGGY 1 cut(s) 2214
BssECI CCNNGG 7 cut(s) 51, 145, 433, 508, 617, 863, 2208
BssMI GATC 6 cut(s) 65, 630, 991, 1177, 1405, 1810
BssSI CACGAG 1 cut(s) 1758
BssT1I CCWWGG 2 cut(s) 617, 2208
Bst2BI CACGAG 1 cut(s) 1758
Bst2UI CCWGG 4 cut(s) 52, 147, 435, 510
Bst4CI ACNGT 4 cut(s) 211, 833, 1272, 2187
Bst6I CTCTTC 3 cut(s) 29, 38, 2268
BstAFI CTTAAG 1 cut(s) 1322
BstAUI TGTACA 1 cut(s) 334
BstC8I GCNNGC 5 cut(s) 1253, 1284, 2195, 2220, 2283
BstDEI CTNAG 8 cut(s) 358, 591, 1064, 1212, 1540, 1717, 2116, 2274
BstF5I GGATG 7 cut(s) 91, 456, 923, 1366, 1653, 1993, 2228
BstHHI GCGC 1 cut(s) 295
BstKTI GATC 6 cut(s) 68, 633, 994, 1180, 1408, 1813
BstMAI GTCTC 2 cut(s) 231, 260
BstMBI GATC 6 cut(s) 65, 630, 991, 1177, 1405, 1810
BstMWI GCNNNNNNNGC 5 cut(s) 718, 822, 1007, 1217, 1283
BstNI CCWGG 4 cut(s) 52, 147, 435, 510
BstNSI RCATGY 4 cut(s) 1255, 1945, 2087, 2096
BstSCI CCNGG 6 cut(s) 50, 145, 433, 508, 862, 1386
BstSFI CTRYAG 2 cut(s) 285, 417
BstV1I GCAGC 6 cut(s) 132, 1022, 1436, 1548, 2290, 2302
BstV2I GAAGAC 2 cut(s) 759, 2284
BstX2I RGATCY 3 cut(s) 991, 1177, 1810
BstYI RGATCY 3 cut(s) 991, 1177, 1810
BsuI GTATCC 1 cut(s) 340
BsuRI GGCC 4 cut(s) 507, 1169, 1286, 2218
BtgZI GCGATG 1 cut(s) 2118
BtsCI GGATG 7 cut(s) 91, 456, 923, 1366, 1653, 1993, 2228
BtsI GCAGTG 2 cut(s) 1965, 2317
BtsIMutI CAGTG 5 cut(s) 216, 312, 838, 1965, 2317
Cac8I GCNNGC 5 cut(s) 1253, 1284, 2195, 2220, 2283
CfoI GCGC 1 cut(s) 295
Cfr10I RCCGGY 1 cut(s) 2214
Cfr13I GGNCC 4 cut(s) 506, 1285, 1894, 2212
CseI GACGC 1 cut(s) 1007
Csp6I GTAC 5 cut(s) 335, 1035, 1057, 1292, 2009
CviQI GTAC 5 cut(s) 335, 1035, 1057, 1292, 2009
DdeI CTNAG 8 cut(s) 358, 591, 1064, 1212, 1540, 1717, 2116, 2274
DpnI GATC 6 cut(s) 67, 632, 993, 1179, 1407, 1812
DpnII GATC 6 cut(s) 65, 630, 991, 1177, 1405, 1810
EaeI YGGCCR 1 cut(s) 2216
Eam1104I CTCTTC 3 cut(s) 29, 38, 2268
EarI CTCTTC 3 cut(s) 29, 38, 2268
Ecl136II GAGCTC 1 cut(s) 2372
Eco130I CCWWGG 2 cut(s) 617, 2208
Eco24I GRGCYC 1 cut(s) 2374
Eco31I GGTCTC 2 cut(s) 231, 260
Eco47I GGWCC 2 cut(s) 1894, 2212
Eco53kI GAGCTC 1 cut(s) 2372
Eco57I CTGAAG 3 cut(s) 124, 1225, 2278
Eco88I CYCGRG 1 cut(s) 1786
EcoICRI GAGCTC 1 cut(s) 2372
EcoRI GAATTC 1 cut(s) 1559
EcoRII CCWGG 4 cut(s) 50, 145, 433, 508
EcoT14I CCWWGG 2 cut(s) 617, 2208
EcoT22I ATGCAT 1 cut(s) 2174
EcoT38I GRGCYC 1 cut(s) 2374
ErhI CCWWGG 2 cut(s) 617, 2208
FaqI GGGAC 3 cut(s) 68, 533, 1907
FauNDI CATATG 1 cut(s) 331
FblI GTMKAC 1 cut(s) 859
Fnu4HI GCNGC 7 cut(s) 121, 1011, 1221, 1425, 1537, 2279, 2316
FokI GGATG 7 cut(s) 98, 463, 930, 1373, 1660, 2000, 2215
FriOI GRGCYC 1 cut(s) 2374
Fsp4HI GCNGC 7 cut(s) 121, 1011, 1221, 1425, 1537, 2279, 2316
FspBI CTAG 7 cut(s) 156, 242, 470, 1289, 1418, 1533, 1652
GlaI GCGC 1 cut(s) 294
GluI GCNGC 7 cut(s) 121, 1011, 1221, 1425, 1537, 2279, 2316
HaeIII GGCC 4 cut(s) 507, 1169, 1286, 2218
HapII CCGG 4 cut(s) 863, 1170, 1388, 2215
HgaI GACGC 1 cut(s) 1007
HhaI GCGC 1 cut(s) 295
Hin6I GCGC 1 cut(s) 293
HinP1I GCGC 1 cut(s) 293
HindIII AAGCTT 2 cut(s) 1613, 1678
HpaII CCGG 4 cut(s) 863, 1170, 1388, 2215
HphI GGTGA 9 cut(s) 211, 617, 762, 997, 1502, 1568, 1769, 1938, 2264
Hpy166II GTNNAC 4 cut(s) 98, 860, 929, 1159
Hpy188I TCNGA 8 cut(s) 202, 459, 635, 913, 1410, 1864, 1987, 2297
Hpy188III TCNNGA 7 cut(s) 370, 995, 1243, 1542, 1788, 1904, 2164
Hpy8I GTNNAC 4 cut(s) 98, 860, 929, 1159
HpyAV CCTTC 9 cut(s) 748, 956, 967, 1100, 1330, 1457, 1504, 1722, 2099
HpyCH4III ACNGT 4 cut(s) 211, 833, 1272, 2187
HpyCH4IV ACGT 1 cut(s) 552
HpyF10VI GCNNNNNNNGC 5 cut(s) 718, 822, 1007, 1217, 1283
HpyF3I CTNAG 8 cut(s) 358, 591, 1064, 1212, 1540, 1717, 2116, 2274
HpySE526I ACGT 1 cut(s) 552
HspAI GCGC 1 cut(s) 293
Kzo9I GATC 6 cut(s) 65, 630, 991, 1177, 1405, 1810
LmnI GCTCC 2 cut(s) 546, 2369
Lsp1109I GCAGC 6 cut(s) 132, 1022, 1436, 1548, 2290, 2302
LweI GCATC 6 cut(s) 280, 727, 1010, 1638, 2159, 2267
MaeI CTAG 7 cut(s) 156, 242, 470, 1289, 1418, 1533, 1652
MaeII ACGT 1 cut(s) 552
MaeIII GTNAC 5 cut(s) 158, 556, 785, 1494, 1944
MalI GATC 6 cut(s) 67, 632, 993, 1179, 1407, 1812
MboI GATC 6 cut(s) 65, 630, 991, 1177, 1405, 1810
MflI RGATCY 3 cut(s) 991, 1177, 1810
MhlI GDGCHC 1 cut(s) 2374
MlyI GAGTC 3 cut(s) 942, 1679, 1778
MmeI TCCRAC 3 cut(s) 324, 872, 1906
Mph1103I ATGCAT 1 cut(s) 2174
MroXI GAANNNNTTC 2 cut(s) 1072, 2302
MseI TTAA 8 cut(s) 428, 482, 1116, 1323, 1449, 1793, 2343, 2351
MslI CAYNNNNRTG 1 cut(s) 804
MspA1I CMGCKG 1 cut(s) 340
MspCI CTTAAG 1 cut(s) 1322
MspI CCGG 4 cut(s) 863, 1170, 1388, 2215
MspR9I CCNGG 6 cut(s) 52, 147, 435, 510, 864, 1388
Mva1269I GAATGC 3 cut(s) 1222, 2172, 2335
MvaI CCWGG 4 cut(s) 52, 147, 435, 510
MwoI GCNNNNNNNGC 5 cut(s) 718, 822, 1007, 1217, 1283
NciI CCSGG 2 cut(s) 864, 1388
NdeI CATATG 1 cut(s) 331
NdeII GATC 6 cut(s) 65, 630, 991, 1177, 1405, 1810
NlaIV GGNNCC 4 cut(s) 319, 548, 808, 1895
NmuCI GTSAC 3 cut(s) 556, 785, 1944
NsiI ATGCAT 1 cut(s) 2174
NspI RCATGY 4 cut(s) 1255, 1945, 2087, 2096
PaeI GCATGC 1 cut(s) 1255
PaeR7I CTCGAG 1 cut(s) 1786
PciI ACATGT 2 cut(s) 1941, 2083
PctI GAATGC 3 cut(s) 1222, 2172, 2335
PdmI GAANNNNTTC 2 cut(s) 1072, 2302
PfeI GAWTC 8 cut(s) 387, 454, 1236, 1603, 1971, 2129, 2204, 2321
PkrI GCNGC 7 cut(s) 122, 1012, 1222, 1426, 1538, 2280, 2317
PleI GAGTC 3 cut(s) 941, 1679, 1778
PpsI GAGTC 3 cut(s) 941, 1679, 1778
PscI ACATGT 2 cut(s) 1941, 2083
PsiI TTATAA 3 cut(s) 258, 579, 1485
Psp124BI GAGCTC 1 cut(s) 2374
Psp6I CCWGG 4 cut(s) 50, 145, 433, 508
PspGI CCWGG 4 cut(s) 50, 145, 433, 508
PspN4I GGNNCC 4 cut(s) 319, 548, 808, 1895
PspPI GGNCC 4 cut(s) 506, 1285, 1894, 2212
PstI CTGCAG 1 cut(s) 289
PsuI RGATCY 3 cut(s) 991, 1177, 1810
PvuII CAGCTG 1 cut(s) 340
RsaI GTAC 5 cut(s) 336, 1036, 1058, 1293, 2010
RsaNI GTAC 5 cut(s) 335, 1035, 1057, 1292, 2009
RseI CAYNNNNRTG 1 cut(s) 804
SacI GAGCTC 1 cut(s) 2374
SaqAI TTAA 8 cut(s) 428, 482, 1116, 1323, 1449, 1793, 2343, 2351
SatI GCNGC 7 cut(s) 121, 1011, 1221, 1425, 1537, 2279, 2316
Sau3AI GATC 6 cut(s) 65, 630, 991, 1177, 1405, 1810
Sau96I GGNCC 4 cut(s) 506, 1285, 1894, 2212
ScaI AGTACT 2 cut(s) 1058, 1293
SchI GAGTC 3 cut(s) 942, 1679, 1778
ScrFI CCNGG 6 cut(s) 52, 147, 435, 510, 864, 1388
SduI GDGCHC 1 cut(s) 2374
SfaNI GCATC 6 cut(s) 280, 727, 1010, 1638, 2159, 2267
SfcI CTRYAG 2 cut(s) 285, 417
Sfr274I CTCGAG 1 cut(s) 1786
SinI GGWCC 2 cut(s) 1894, 2212
SlaI CTCGAG 1 cut(s) 1786
SmiMI CAYNNNNRTG 1 cut(s) 804
SmlI CTYRAG 6 cut(s) 61, 80, 1322, 1367, 1786, 2164
SmoI CTYRAG 6 cut(s) 61, 80, 1322, 1367, 1786, 2164
SpeI ACTAGT 1 cut(s) 1417
SphI GCATGC 1 cut(s) 1255
SsiI CCGC 2 cut(s) 163, 1221
SspI AATATT 2 cut(s) 1828, 1884
SspMI CTAG 7 cut(s) 156, 242, 470, 1289, 1418, 1533, 1652
SstI GAGCTC 1 cut(s) 2374
StyD4I CCNGG 6 cut(s) 50, 145, 433, 508, 862, 1386
StyI CCWWGG 2 cut(s) 617, 2208
TaaI ACNGT 4 cut(s) 211, 833, 1272, 2187
TaiI ACGT 1 cut(s) 555
TaqI TCGA 7 cut(s) 68, 629, 846, 1004, 1659, 1787, 2374
TatI WGTACW 4 cut(s) 334, 1056, 1291, 2008
TauI GCSGC 1 cut(s) 1223
TfiI GAWTC 8 cut(s) 387, 454, 1236, 1603, 1971, 2129, 2204, 2321
Tru1I TTAA 8 cut(s) 428, 482, 1116, 1323, 1449, 1793, 2343, 2351
Tru9I TTAA 8 cut(s) 428, 482, 1116, 1323, 1449, 1793, 2343, 2351
TscAI CASTG 5 cut(s) 216, 319, 838, 1965, 2317
TseFI GTSAC 3 cut(s) 556, 785, 1944
TseI GCWGC 6 cut(s) 120, 1010, 1424, 1536, 2278, 2315
Tsp45I GTSAC 3 cut(s) 556, 785, 1944
TspGWI ACGGA 1 cut(s) 2221
TspRI CASTG 5 cut(s) 216, 319, 838, 1965, 2317
Vha464I CTTAAG 1 cut(s) 1322
VpaK11BI GGWCC 2 cut(s) 1894, 2212
XapI RAATTY 4 cut(s) 151, 1559, 1857, 2302
XceI RCATGY 4 cut(s) 1255, 1945, 2087, 2096
XhoI CTCGAG 1 cut(s) 1786
XmiI GTMKAC 1 cut(s) 859
XmnI GAANNNNTTC 2 cut(s) 1072, 2302
XspI CTAG 7 cut(s) 156, 242, 470, 1289, 1418, 1533, 1652
ZrmI AGTACT 2 cut(s) 1058, 1293
Zsp2I ATGCAT 1 cut(s) 2174
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.