Rh2DG381200

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0000112
Physical Location & Seq
Forward (+)
54945799 .. 54949446
3648 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG381200.1

Sequence Viewer

Length: 1629 bp
ATGAAAATAAGAAAAAAGATGCCATTGGTTCATTCCTACAGAATTGAAAATGCCGAGAAAATGGTTTTGAAAGCTCTGCTTCTCTTCCTCCTCTTTCAATTATGCACTTCCAGGGACACCATGAGAGGGAACGAAGAACAATTGAAGGATGTTGATGGTGACTATTTAGTGTCCAAAGAAAGCAAGTTTGAATTGGGTTTCTTCAGCCCCGGAAATTCTAGCAACCGGTATGTTGGAGTCTGGTATTCTCAGACTAGAGTATCTAATAAAACAGTGGTGTGGGTTGCAAACAGGAACAATCCTATCAATGATACCTCTGGTGTGCTCACAATAAACAGGTATGGAGAACTAGTCCTTTATGCTCATAACATGGAAAGCACTCCTATTTGGTCTACTAACGTGTCGCGGTCGGTTCAAAATGTTAACACAAGCACTTCATCTACACAGCTTTTAGATACAGGAAATTTAGTTGTGTTCCAGGATGATAAAAATGGAATCTTTATATGGCAAAGTTTTGATCATCCTATAGATACTTTAATTCCAGGTATGAAAGTTGGGGTGAATTGGAAAACTGGGCAAGAATGGGTTTTAACATCTTGGAAGTCACAAGATGACCCTGGAACTGGGGACTATACCTATAGGCTAAGTTCAGATCATAATGCATCTCCCCAATTTTTTTTGTATAAAGGTTTGAGTAAGTATTGGCGAAGTGATCCAGGGCCAGCGCCTACTTTGGTCGCTAATCAAGATGAAACTTATTTTTTCATGAATGAAACCAATGCAATTACAAGAATAACAGTGACTGATTCTGCCTTAGAGCACCTTATATGGGATGGTGGTGGCTTTCAATGGAAGGAAGACTTCTCTGCACCGAAGTCCCGGTGTGACAGGTACGGACAGTGTGGTGCCAACAGCAGATGTAGCCCTGACAATGTTAATCTGTTTGAGTGTGACTGTTTGCCAGGGTATGTGCCTAATTCTGTAAGTGATTGGAATCAGAAAAATGGTTCAGGTGGATGTGTGAGTAATCGACTTGGTTTGTTGAAGTGTGGAGATGGAGACGGGTTTATAAAGGTGGCAAGAGTTAAATATCCAGACACAACGATAGCAGCATCGTTAAAATCAGGTATGAGTGACAAAGAGTGTGCACAGGAGTGCCTAAGAAATTGTTCTTGCACTGCATATTTGAGCACTGAAAATGAAGGGATTGTTGATTGCTTGACATGGTATGATGACTTGATGGACATTTTAGTGTACACAGGGCTTGGACGAGATCTCTATGTTCGTGTGAATGCAACTGTGTTAGCGGCAAATGCCGGAAGATCACAAGGTTTCTGGGAAAGGAGGGGTATGCTGGCTATTCCAATACTGTCTACTGCGCTAGCATTGGTACTAATCATTATGCTTGCATGGAAGTGGCATAAGAAGAACAGGAACACAAGAGGCATAGACTTTGTGGAGGCAGAGGAGCTTGAGGAAACTCAGAGACACCCCGAATTGCAATTTTTCGATCTTGACACAATAATAGCAGCCACAGACCACTTCTCTCGTGTCAATGAACTTGGCCGTGGTGGCTTTGGCTCTGTTTATAAGGTAAACAACAAATTTAGATGTCCTAAACATTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

542

Amino Acids

61.14

Weight (kDa)

6.19

Isoelectric Point (pI)

31.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 91 - 201 2e-35 D-mannose binding lectin
S_locus_glycop PF00954 249 - 326 2.2e-12 S-locus glycoprotein domain
PAN_2 PF08276 350 - 416 1.8e-17 PAN-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1070, 1590
AccB1I GGYRCC 1 cut(s) 905
AccI GTMKAC 2 cut(s) 392, 1373
AccII CGCG 1 cut(s) 406
AciI CCGC 2 cut(s) 406, 1307
AclWI GGATC 1 cut(s) 707
AcoI YGGCCR 1 cut(s) 1564
AcsI RAATTY 3 cut(s) 214, 463, 1604
AcuI CTGAAG 1 cut(s) 187
AfaI GTAC 3 cut(s) 893, 1256, 1392
AfiI CCNNNNNNNGG 3 cut(s) 126, 623, 829
AflIII ACRYGT 1 cut(s) 399
AgeI ACCGGT 1 cut(s) 225
AgsI TTSAA 8 cut(s) 47, 70, 98, 145, 191, 416, 848, 1045
AhlI ACTAGT 1 cut(s) 349
AjnI CCWGG 6 cut(s) 110, 477, 541, 616, 715, 961
AleI CACNNNNGTG 1 cut(s) 1153
AluBI AGCT 3 cut(s) 74, 448, 1471
AluI AGCT 3 cut(s) 74, 448, 1471
Alw21I GWGCWC 4 cut(s) 327, 822, 1150, 1193
Alw26I GTCTC 2 cut(s) 1053, 1480
Alw44I GTGCAC 1 cut(s) 1146
AlwI GGATC 1 cut(s) 707
AlwNI CAGNNNCTG 1 cut(s) 803
AoxI GGCC 2 cut(s) 719, 1564
ApaLI GTGCAC 1 cut(s) 1146
ApeKI GCWGC 2 cut(s) 1109, 1529
ApoI RAATTY 3 cut(s) 214, 463, 1604
AsiGI ACCGGT 1 cut(s) 225
Asp700I GAANNNNTTC 1 cut(s) 1168
AspLEI GCGC 2 cut(s) 727, 1381
AspS9I GGNCC 1 cut(s) 719
AsuC2I CCSGG 2 cut(s) 210, 880
AsuHPI GGTGA 2 cut(s) 170, 571
AsuNHI GCTAGC 1 cut(s) 1381
BaeGI GKGCMC 1 cut(s) 1150
BaeI ACNNNNGTAYC 2 cut(s) 243, 276
BanI GGYRCC 1 cut(s) 905
BauI CACGAG 1 cut(s) 1548
BbsI GAAGAC 1 cut(s) 864
Bbv12I GWGCWC 4 cut(s) 327, 822, 1150, 1193
BbvI GCAGC 2 cut(s) 1121, 1541
BccI CCATC 4 cut(s) 149, 827, 1049, 1234
BceAI ACGGC 1 cut(s) 1551
BciT130I CCWGG 6 cut(s) 112, 479, 543, 618, 717, 963
BclI TGATCA 1 cut(s) 517
BcnI CCSGG 2 cut(s) 210, 880
BcoDI GTCTC 2 cut(s) 1053, 1480
BcuI ACTAGT 1 cut(s) 349
BfaI CTAG 4 cut(s) 219, 255, 350, 1382
BfmI CTRYAG 3 cut(s) 37, 525, 637
BfoI RGCGCY 1 cut(s) 728
BglI GCCNNNNNGGC 1 cut(s) 1572
BglII AGATCT 1 cut(s) 1273
BisI GCNGC 3 cut(s) 1110, 1308, 1530
BlsI GCNGC 3 cut(s) 1111, 1309, 1531
Bme1390I CCNGG 8 cut(s) 112, 210, 479, 543, 618, 717, 880, 963
BmgT120I GGNCC 1 cut(s) 719
BmiI GGNNCC 1 cut(s) 907
BmrFI CCNGG 8 cut(s) 112, 210, 479, 543, 618, 717, 880, 963
BmrI ACTGGG 2 cut(s) 582, 633
BmsI GCATC 3 cut(s) 9, 671, 1121
BmtI GCTAGC 1 cut(s) 1385
BmuI ACTGGG 2 cut(s) 582, 633
BpiI GAAGAC 1 cut(s) 864
BpuEI CTTGAG 1 cut(s) 1493
BpuMI CCSGG 2 cut(s) 210, 880
BsaBI GATNNNNATC 1 cut(s) 993
BsaJI CCNNGG 6 cut(s) 111, 208, 616, 716, 962, 1567
BsaWI WCCGGW 1 cut(s) 225
BsaXI ACNNNNNCTCC 2 cut(s) 336, 366
Bsc4I CCNNNNNNNGG 3 cut(s) 126, 623, 829
Bse118I RCCGGY 1 cut(s) 225
Bse1I ACTGG 2 cut(s) 577, 628
Bse8I GATNNNNATC 1 cut(s) 993
BseBI CCWGG 6 cut(s) 112, 479, 543, 618, 717, 963
BseDI CCNNGG 6 cut(s) 111, 208, 616, 716, 962, 1567
BseGI GGATG 5 cut(s) 154, 487, 520, 838, 1022
BseJI GATNNNNATC 1 cut(s) 993
BseLI CCNNNNNNNGG 3 cut(s) 126, 623, 829
BseMII CTCAG 2 cut(s) 263, 1496
BseNI ACTGG 2 cut(s) 577, 628
BseRI GAGGAG 2 cut(s) 80, 1481
BseSI GKGCMC 1 cut(s) 1150
BseXI GCAGC 2 cut(s) 1121, 1541
BsgI GTGCAG 1 cut(s) 852
Bsh1236I CGCG 1 cut(s) 406
Bsh1285I CGRYCG 1 cut(s) 410
BshFI GGCC 2 cut(s) 721, 1566
BshNI GGYRCC 1 cut(s) 905
BshTI ACCGGT 1 cut(s) 225
BsiEI CGRYCG 1 cut(s) 410
BsiHKAI GWGCWC 4 cut(s) 327, 822, 1150, 1193
BsiSI CCGG 4 cut(s) 210, 226, 880, 1317
BslFI GGGAC 3 cut(s) 128, 641, 862
BslI CCNNNNNNNGG 3 cut(s) 126, 623, 829
BsmAI GTCTC 2 cut(s) 1053, 1480
BsmBI CGTCTC 1 cut(s) 1053
BsmFI GGGAC 3 cut(s) 128, 641, 862
BsmI GAATGC 1 cut(s) 1297
BsnI GGCC 2 cut(s) 721, 1566
Bsp1286I GDGCHC 4 cut(s) 327, 822, 1150, 1193
Bsp1407I TGTACA 1 cut(s) 1254
Bsp143I GATC 6 cut(s) 517, 652, 712, 1273, 1322, 1510
BspACI CCGC 2 cut(s) 406, 1307
BspANI GGCC 2 cut(s) 721, 1566
BspCNI CTCAG 2 cut(s) 262, 1495
BspFNI CGCG 1 cut(s) 406
BspHI TCATGA 1 cut(s) 765
BspLI GGNNCC 1 cut(s) 907
BspOI GCTAGC 1 cut(s) 1385
BspPI GGATC 1 cut(s) 707
BspT107I GGYRCC 1 cut(s) 905
BsrFI RCCGGY 1 cut(s) 225
BsrGI TGTACA 1 cut(s) 1254
BsrI ACTGG 2 cut(s) 577, 628
BssAI RCCGGY 1 cut(s) 225
BssECI CCNNGG 6 cut(s) 111, 208, 616, 716, 962, 1567
BssMI GATC 6 cut(s) 517, 652, 712, 1273, 1322, 1510
BssSI CACGAG 1 cut(s) 1548
Bst2BI CACGAG 1 cut(s) 1548
Bst2UI CCWGG 6 cut(s) 112, 479, 543, 618, 717, 963
Bst4CI ACNGT 6 cut(s) 274, 799, 900, 956, 1300, 1371
Bst6I CTCTTC 1 cut(s) 89
BstAUI TGTACA 1 cut(s) 1254
BstC8I GCNNGC 4 cut(s) 723, 1356, 1383, 1407
BstDEI CTNAG 5 cut(s) 249, 644, 814, 1160, 1482
BstDSI CCRYGG 1 cut(s) 1567
BstF5I GGATG 5 cut(s) 154, 487, 520, 838, 1022
BstFNI CGCG 1 cut(s) 406
BstH2I RGCGCY 1 cut(s) 728
BstHHI GCGC 2 cut(s) 727, 1381
BstKTI GATC 6 cut(s) 520, 655, 715, 1276, 1325, 1513
BstMAI GTCTC 2 cut(s) 1053, 1480
BstMBI GATC 6 cut(s) 517, 652, 712, 1273, 1322, 1510
BstMCI CGRYCG 1 cut(s) 410
BstMWI GCNNNNNNNGC 3 cut(s) 921, 1313, 1572
BstNI CCWGG 6 cut(s) 112, 479, 543, 618, 717, 963
BstSCI CCNGG 8 cut(s) 110, 208, 477, 541, 616, 715, 878, 961
BstSFI CTRYAG 3 cut(s) 37, 525, 637
BstSLI GKGCMC 1 cut(s) 1150
BstUI CGCG 1 cut(s) 406
BstV1I GCAGC 2 cut(s) 1121, 1541
BstV2I GAAGAC 1 cut(s) 864
BstX2I RGATCY 1 cut(s) 1273
BstYI RGATCY 1 cut(s) 1273
BsuRI GGCC 2 cut(s) 721, 1566
BtgI CCRYGG 1 cut(s) 1567
BtsCI GGATG 5 cut(s) 154, 487, 520, 838, 1022
BtsI GCAGTG 1 cut(s) 1176
BtsIMutI CAGTG 5 cut(s) 279, 804, 905, 1176, 1191
Cac8I GCNNGC 4 cut(s) 723, 1356, 1383, 1407
CaiI CAGNNNCTG 1 cut(s) 803
CciI TCATGA 1 cut(s) 765
CfoI GCGC 2 cut(s) 727, 1381
Cfr10I RCCGGY 1 cut(s) 225
Cfr13I GGNCC 1 cut(s) 719
Csp6I GTAC 3 cut(s) 892, 1255, 1391
CspAI ACCGGT 1 cut(s) 225
CviAII CATG 5 cut(s) 121, 370, 766, 1224, 1410
CviQI GTAC 3 cut(s) 892, 1255, 1391
DdeI CTNAG 5 cut(s) 249, 644, 814, 1160, 1482
DpnI GATC 6 cut(s) 519, 654, 714, 1275, 1324, 1512
DpnII GATC 6 cut(s) 517, 652, 712, 1273, 1322, 1510
EaeI YGGCCR 1 cut(s) 1564
Eam1104I CTCTTC 1 cut(s) 89
EarI CTCTTC 1 cut(s) 89
Eco57I CTGAAG 1 cut(s) 187
EcoRII CCWGG 6 cut(s) 110, 477, 541, 616, 715, 961
EcoT22I ATGCAT 1 cut(s) 664
Esp3I CGTCTC 1 cut(s) 1053
FaeI CATG 5 cut(s) 124, 373, 769, 1227, 1413
FalI AAGNNNNNCTT 4 cut(s) 63, 95, 845, 877
FaqI GGGAC 3 cut(s) 128, 641, 862
FatI CATG 5 cut(s) 120, 369, 765, 1223, 1409
FbaI TGATCA 1 cut(s) 517
FblI GTMKAC 2 cut(s) 392, 1373
Fnu4HI GCNGC 3 cut(s) 1110, 1308, 1530
FokI GGATG 5 cut(s) 161, 494, 507, 845, 1029
Fsp4HI GCNGC 3 cut(s) 1110, 1308, 1530
FspBI CTAG 4 cut(s) 219, 255, 350, 1382
GlaI GCGC 2 cut(s) 726, 1380
GluI GCNGC 3 cut(s) 1110, 1308, 1530
HaeII RGCGCY 1 cut(s) 728
HaeIII GGCC 2 cut(s) 721, 1566
HapII CCGG 4 cut(s) 210, 226, 880, 1317
HhaI GCGC 2 cut(s) 727, 1381
Hin1II CATG 5 cut(s) 124, 373, 769, 1227, 1413
Hin6I GCGC 2 cut(s) 725, 1379
HinP1I GCGC 2 cut(s) 725, 1379
HincII GTYRAC 1 cut(s) 424
HindII GTYRAC 1 cut(s) 424
HinfI GANTC 4 cut(s) 237, 495, 806, 994
HpaI GTTAAC 1 cut(s) 424
HpaII CCGG 4 cut(s) 210, 226, 880, 1317
HphI GGTGA 2 cut(s) 170, 571
Hpy166II GTNNAC 7 cut(s) 393, 424, 1148, 1255, 1257, 1374, 1597
Hpy188I TCNGA 4 cut(s) 252, 652, 999, 1485
Hpy188III TCNNGA 4 cut(s) 746, 766, 1094, 1514
Hpy8I GTNNAC 7 cut(s) 393, 424, 1148, 1255, 1257, 1374, 1597
HpyAV CCTTC 3 cut(s) 139, 847, 1196
HpyCH4III ACNGT 6 cut(s) 274, 799, 900, 956, 1300, 1371
HpyCH4IV ACGT 1 cut(s) 399
HpyF10VI GCNNNNNNNGC 3 cut(s) 921, 1313, 1572
HpyF3I CTNAG 5 cut(s) 249, 644, 814, 1160, 1482
HpySE526I ACGT 1 cut(s) 399
Hsp92II CATG 5 cut(s) 124, 373, 769, 1227, 1413
HspAI GCGC 2 cut(s) 725, 1379
Ksp22I TGATCA 1 cut(s) 517
KspAI GTTAAC 1 cut(s) 424
Kzo9I GATC 6 cut(s) 517, 652, 712, 1273, 1322, 1510
LmnI GCTCC 1 cut(s) 1468
Lsp1109I GCAGC 2 cut(s) 1121, 1541
LweI GCATC 3 cut(s) 9, 671, 1121
MaeI CTAG 4 cut(s) 219, 255, 350, 1382
MaeII ACGT 1 cut(s) 399
MaeIII GTNAC 6 cut(s) 158, 603, 799, 884, 950, 1133
MalI GATC 6 cut(s) 519, 654, 714, 1275, 1324, 1512
MboI GATC 6 cut(s) 517, 652, 712, 1273, 1322, 1510
MboII GAAGA 6 cut(s) 76, 146, 193, 869, 1332, 1438
MfeI CAATTG 1 cut(s) 140
MflI RGATCY 1 cut(s) 1273
MhlI GDGCHC 4 cut(s) 327, 822, 1150, 1193
MlyI GAGTC 1 cut(s) 246
MmeI TCCRAC 1 cut(s) 214
MnlI CCTC 9 cut(s) 98, 101, 119, 325, 1338, 1436, 1453, 1459, 1468
Mph1103I ATGCAT 1 cut(s) 664
MroXI GAANNNNTTC 1 cut(s) 1168
MseI TTAA 6 cut(s) 423, 536, 590, 936, 1086, 1118
MslI CAYNNNNRTG 3 cut(s) 1153, 1250, 1414
MspI CCGG 4 cut(s) 210, 226, 880, 1317
MspR9I CCNGG 8 cut(s) 112, 210, 479, 543, 618, 717, 880, 963
MunI CAATTG 1 cut(s) 140
Mva1269I GAATGC 1 cut(s) 1297
MvaI CCWGG 6 cut(s) 112, 479, 543, 618, 717, 963
MvnI CGCG 1 cut(s) 406
MwoI GCNNNNNNNGC 3 cut(s) 921, 1313, 1572
NciI CCSGG 2 cut(s) 210, 880
NdeII GATC 6 cut(s) 517, 652, 712, 1273, 1322, 1510
NheI GCTAGC 1 cut(s) 1381
NlaIII CATG 5 cut(s) 124, 373, 769, 1227, 1413
NlaIV GGNNCC 1 cut(s) 907
NmeAIII GCCGAG 1 cut(s) 79
NmuCI GTSAC 6 cut(s) 158, 603, 799, 884, 950, 1133
NsiI ATGCAT 1 cut(s) 664
OliI CACNNNNGTG 1 cut(s) 1153
PagI TCATGA 1 cut(s) 765
PctI GAATGC 1 cut(s) 1297
PdmI GAANNNNTTC 1 cut(s) 1168
PfeI GAWTC 3 cut(s) 495, 806, 994
PfoI TCCNGGA 1 cut(s) 477
PinAI ACCGGT 1 cut(s) 225
PkrI GCNGC 3 cut(s) 1111, 1309, 1531
PleI GAGTC 1 cut(s) 245
PpsI GAGTC 1 cut(s) 245
PsiI TTATAA 2 cut(s) 1070, 1590
Psp6I CCWGG 6 cut(s) 110, 477, 541, 616, 715, 961
PspGI CCWGG 6 cut(s) 110, 477, 541, 616, 715, 961
PspN4I GGNNCC 1 cut(s) 907
PspPI GGNCC 1 cut(s) 719
PstNI CAGNNNCTG 1 cut(s) 803
PsuI RGATCY 1 cut(s) 1273
RsaI GTAC 3 cut(s) 893, 1256, 1392
RsaNI GTAC 3 cut(s) 892, 1255, 1391
RseI CAYNNNNRTG 3 cut(s) 1153, 1250, 1414
SaqAI TTAA 6 cut(s) 423, 536, 590, 936, 1086, 1118
SatI GCNGC 3 cut(s) 1110, 1308, 1530
Sau3AI GATC 6 cut(s) 517, 652, 712, 1273, 1322, 1510
Sau96I GGNCC 1 cut(s) 719
SchI GAGTC 1 cut(s) 246
ScrFI CCNGG 8 cut(s) 112, 210, 479, 543, 618, 717, 880, 963
SduI GDGCHC 4 cut(s) 327, 822, 1150, 1193
SfaNI GCATC 3 cut(s) 9, 671, 1121
SfcI CTRYAG 3 cut(s) 37, 525, 637
SmiMI CAYNNNNRTG 3 cut(s) 1153, 1250, 1414
SmlI CTYRAG 1 cut(s) 1472
SmoI CTYRAG 1 cut(s) 1472
SpeI ACTAGT 1 cut(s) 349
SsiI CCGC 2 cut(s) 406, 1307
SspMI CTAG 4 cut(s) 219, 255, 350, 1382
StyD4I CCNGG 8 cut(s) 110, 208, 477, 541, 616, 715, 878, 961
TaaI ACNGT 6 cut(s) 274, 799, 900, 956, 1300, 1371
TaiI ACGT 1 cut(s) 402
TaqI TCGA 2 cut(s) 1030, 1509
TatI WGTACW 1 cut(s) 1254
TauI GCSGC 1 cut(s) 1310
TfiI GAWTC 3 cut(s) 495, 806, 994
Tru1I TTAA 6 cut(s) 423, 536, 590, 936, 1086, 1118
Tru9I TTAA 6 cut(s) 423, 536, 590, 936, 1086, 1118
TscAI CASTG 5 cut(s) 279, 804, 905, 1183, 1198
TseFI GTSAC 6 cut(s) 158, 603, 799, 884, 950, 1133
TseI GCWGC 2 cut(s) 1109, 1529
Tsp45I GTSAC 6 cut(s) 158, 603, 799, 884, 950, 1133
TspGWI ACGGA 1 cut(s) 909
TspRI CASTG 5 cut(s) 279, 804, 905, 1183, 1198
VneI GTGCAC 1 cut(s) 1146
XapI RAATTY 3 cut(s) 214, 463, 1604
XmiI GTMKAC 2 cut(s) 392, 1373
XmnI GAANNNNTTC 1 cut(s) 1168
XspI CTAG 4 cut(s) 219, 255, 350, 1382
Zsp2I ATGCAT 1 cut(s) 664
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.