Rw3G018870

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000112
Physical Location & Seq
Forward (+)
21171740 .. 21174957
3218 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G018870.1

Sequence Viewer

Length: 2433 bp
ATGTTATTGAAATCTTTGCTTCTCTTCCGCCTCTTCCAATTTTGCACTTGCAGGGACACCATCAGATGGAATGAACAACGACTGAAGGATGTTGATGGTGATGTTTTGGTGTCCAAAGAAAGCAACTTTGAGTTGGGTTTCTTCAGCCCCGGCGATTCTAGCCACCGGTACGTTGGTATTTGGTATTCTCAATCTAAAATATCTGCAAAAACAGTGGTGTGGATTGCAAACAGAGACAACCCCATCAATGATACCTCTGGAGTTCTCACAATGAACAGGTATGGGGAATTAGCTCTTTATGCTTATAACATGGAGAGCATTCCTGTATGGTCTACAAATGTATCACAGTCGGTCCAAAACACAGACACCTTATATAGATATGTCCAGCTTTTGGATACTGGAAACTTAATTCTGTTCCGGGATGGTAGAAATGGAAGCTTGATTTTATGGCAAAGTTTTGATTATCCTGCAGATACTCTAATCCCAGGTATGAAAGTTGGGTTGAATTGGAAAAAAGGTCTAGATTGGGTTTTAACATCTTGGAAGTCACAAGATGACCCTGGAAAGGGGGACTATGCCTTTAGGGTCGCTCCGAATCACAATGCTACTCCCCAATATTTTGTCTACAAGGGTTTCACTAAGTATTGGCGAATGCATGCAGGGCCATGGCCTAATTTTGTTAGTAATGCCAACGAACTGTATAATACCCTGCCTTATAGGAGCGGTGCTATTTCAATAGTAAGGATGAAGGATTCCGGGTTACTTCAGTACCTAACATGGAATGATGCTGACCATCAGTGGGTGGAAATGTGGTCTTCGCCGAAATACCCTTGTGACTGGTATGGACATTGTGGTGCCAACAGCAGATGTAGGCCTGACAATATTAATTTATTTGAGTGTGAGTGTATTCCAGGGTATGAGCCAAAATCTGTAGATGGTTGGAATCGGAGAGATGGAACAGATGGATGTGTGAGTAAACGAGTAAGAGTGTCCAAGTGTAGGAATGGAGAAGGGTTTGTGAAGGTGGCAAGGGTAAAAGATCCAGACACATCGAATGTAGCAAGGTTGAGAAAAAGTATGAGTGCCAAAGCGTGTGAGCAGGAGTGCTTGAAAAATTGTTCTTGCACGGCATATATGAGCATTACACTGGAAAGGGTTACCAATTGCTTGACATGGTATGATGACTTGATGGACATGTTAGTTTACACAGGGGCCGGACGAGATCTATATGTTCGTGTGGATAAAATTTCATTAGCTGAGGCCAGAAACTCACAAGGTTTCTTCAGGACTGGCATGGTCCTTATTCCAGTACTAACTGCTCTGGTGGCACTACTACTAATCATTATACTTTCCCTGTGGTTGCTTAAGAAAAAGAGGAAGACAAGAGACTATCTGGATGCGGAGGAACTTGAGGAAACTAAGAGACACCCGGAATTGCAATTTTTCGATCTTAACACACTGATAGCAGCCACATACAACTTCTCTGATGCCAATAAACTTGGCCAAGGTGGTTTTGGCAGTGTTTATAAGGGCGAATTACCAAACGAACAAAAAGTTGCTATAAAAAGATTGTCTAGAACTTCAGGACAGGGAATCGAAGAATTCAAAAACGAAGTTGCACTAATAGCCAGACTTCAGCACAGGAATCTTGTGAAACTTTTAGGCTGCTGTATAAAGGGAGAAGAAAGGATATTGGTTCTGGAATACATGCCGAACAAAAGCTTGGACTCCTTTCTTTTTGATAACACAAGGAGGTCACTTTTAAATTGGGAAAAGCGTTTTGAAATTATCAATGGGGTTGCTCGTGGGGTTCTATATCTTCATCGGGACTCAAGATTGAGGGTTATCCATAGGGATCTAAAACCCGGTAATGTCCTTCTAGATGCCGAGTTAGACCCAAAAATTTCTGATTTCGGCATGGCAAGAATATTTCAAGGGGACCAACTCCTGGAAAAGACAAACAGAGTTGTCGGAACATACGGCTACATGTCACCAGAATATGCAGTATTTGGGAGATTTTCTACGAAATCGGATGTGTTTAGTTTTGGGATCATACTATTGGAGATTGTTAGTGGCAAGAGGAACAATGGTTCTTATGAGATGCATTATTCCATAAACTTGATAGGACATGTATGGCAACTTTGGGTAGAAGACAAAGTCTTGGAAATTGTGGATTCATCACTAGAGATTTATCAGCTTGATGAAGTCTTGAGATGCATTCAAGTTGGGCTTTTGTGCGTGCAAGAAGATTTGAAGGACCGACCAGCCATGTCAGCCGTCGTGTTCATGTTGAGTGGTGAAACATCTCTTCCATCTCCTAAGCAGCCTGCATTTGTTTTCAGAACAAGTTCCAGCATTGTTGATGATCCCTTCATTCCAAATGAATCATATTCTAGTAATAGTTTGACAATAACTAACATGGAAGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

810

Amino Acids

92.57

Weight (kDa)

8.12

Isoelectric Point (pI)

38.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 71 - 182 1.7e-32 D-mannose binding lectin
S_locus_glycop PF00954 226 - 310 1.1e-14 S-locus glycoprotein domain
PAN_2 PF08276 333 - 399 5.8e-16 PAN-like domain
Pkinase PF00069 495 - 695 3.3e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 498 - 761 1.7e-48 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 767 - 810 3.7e-06 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 306, 1527
AccB1I GGYRCC 1 cut(s) 854
AccB7I CCANNNNNTGG 3 cut(s) 66, 391, 1948
AccBSI CCGCTC 1 cut(s) 723
AccI GTMKAC 2 cut(s) 332, 624
AciI CCGC 3 cut(s) 28, 723, 1400
AclWI GGATC 4 cut(s) 1034, 1863, 2057, 2360
AcoI YGGCCR 1 cut(s) 1501
AcsI RAATTY 3 cut(s) 1245, 1601, 1902
AcuI CTGAAG 6 cut(s) 104, 127, 749, 1267, 1566, 1619
AfaI GTAC 3 cut(s) 170, 770, 1311
AfiI CCNNNNNNNGG 7 cut(s) 66, 391, 565, 566, 799, 999, 1948
AflII CTTAAG 1 cut(s) 1364
AflIII ACRYGT 3 cut(s) 1194, 1986, 2128
AgeI ACCGGT 1 cut(s) 165
AgsI TTSAA 9 cut(s) 10, 505, 735, 1111, 1606, 1784, 1934, 2222, 2254
AjnI CCWGG 4 cut(s) 484, 559, 910, 1947
AjuI GAANNNNNNNTTGG 2 cut(s) 1706, 1738
AloI GAACNNNNNNTCC 2 cut(s) 2074, 2106
AluBI AGCT 7 cut(s) 293, 388, 438, 1256, 1722, 2197, 2426
AluI AGCT 7 cut(s) 293, 388, 438, 1256, 1722, 2197, 2426
Alw26I GTCTC 3 cut(s) 228, 1380, 1417
AlwI GGATC 4 cut(s) 1034, 1863, 2057, 2360
AoxI GGCC 6 cut(s) 662, 668, 872, 1212, 1260, 1501
ApeKI GCWGC 3 cut(s) 1466, 1665, 2323
ApoI RAATTY 3 cut(s) 1245, 1601, 1902
AseI ATTAAT 1 cut(s) 885
AsiGI ACCGGT 1 cut(s) 165
Asp700I GAANNNNTTC 1 cut(s) 2347
AspS9I GGNCC 6 cut(s) 352, 662, 1212, 1297, 1939, 2257
AsuC2I CCSGG 5 cut(s) 150, 419, 757, 1430, 1866
AsuHPI GGTGA 3 cut(s) 110, 1983, 2309
AvaII GGWCC 4 cut(s) 352, 1297, 1939, 2257
BaeI ACNNNNGTAYC 6 cut(s) 160, 193, 324, 357, 752, 785
BalI TGGCCA 1 cut(s) 1503
BanI GGYRCC 1 cut(s) 854
BauI CACGAG 1 cut(s) 1803
BbsI GAAGAC 3 cut(s) 807, 1385, 2157
BbvCI CCTCAGC 1 cut(s) 1257
BbvI GCAGC 3 cut(s) 1478, 1652, 2335
BceAI ACGGC 3 cut(s) 1143, 1996, 2261
BciT130I CCWGG 4 cut(s) 486, 561, 912, 1949
BciVI GTATCC 1 cut(s) 388
BcnI CCSGG 5 cut(s) 150, 419, 757, 1430, 1866
BcoDI GTCTC 3 cut(s) 228, 1380, 1417
BfaI CTAG 6 cut(s) 159, 521, 1575, 1880, 2183, 2394
BfmI CTRYAG 2 cut(s) 468, 930
BfrI CTTAAG 1 cut(s) 1364
BfuI GTATCC 1 cut(s) 388
BglII AGATCT 1 cut(s) 1222
BisI GCNGC 3 cut(s) 1467, 1666, 2324
BlsI GCNGC 3 cut(s) 1468, 1667, 2325
BmcAI AGTACT 1 cut(s) 1311
Bme1390I CCNGG 9 cut(s) 150, 419, 486, 561, 757, 912, 1430, 1866, 1949
Bme18I GGWCC 4 cut(s) 352, 1297, 1939, 2257
BmgT120I GGNCC 6 cut(s) 352, 662, 1212, 1297, 1939, 2257
BmiI GGNNCC 3 cut(s) 856, 1213, 1940
BmrFI CCNGG 9 cut(s) 150, 419, 486, 561, 757, 912, 1430, 1866, 1949
BmsI GCATC 6 cut(s) 775, 1387, 1477, 1873, 2091, 2204
BpiI GAAGAC 3 cut(s) 807, 1385, 2157
BpmI CTGGAG 1 cut(s) 279
Bpu10I CCTNAGC 2 cut(s) 1257, 2319
BpuEI CTTGAG 3 cut(s) 1430, 1816, 2230
BpuMI CCSGG 5 cut(s) 150, 419, 757, 1430, 1866
BsaJI CCNNGG 6 cut(s) 148, 484, 559, 665, 911, 1504
BsaWI WCCGGW 1 cut(s) 165
Bsc4I CCNNNNNNNGG 7 cut(s) 66, 391, 565, 566, 799, 999, 1948
Bse118I RCCGGY 1 cut(s) 165
Bse1I ACTGG 5 cut(s) 403, 842, 1152, 1294, 1307
BseBI CCWGG 4 cut(s) 486, 561, 912, 1949
BseDI CCNNGG 6 cut(s) 148, 484, 559, 665, 911, 1504
BseGI GGATG 6 cut(s) 94, 427, 750, 971, 1402, 2038
BseLI CCNNNNNNNGG 7 cut(s) 66, 391, 565, 566, 799, 999, 1948
BseMII CTCAG 1 cut(s) 1248
BseNI ACTGG 5 cut(s) 403, 842, 1152, 1294, 1307
BseXI GCAGC 3 cut(s) 1478, 1652, 2335
BshFI GGCC 6 cut(s) 664, 670, 874, 1214, 1262, 1503
BshNI GGYRCC 1 cut(s) 854
BshTI ACCGGT 1 cut(s) 165
BsiSI CCGG 7 cut(s) 150, 166, 418, 756, 1215, 1430, 1866
BslFI GGGAC 4 cut(s) 68, 584, 1841, 1952
BslI CCNNNNNNNGG 7 cut(s) 66, 391, 565, 566, 799, 999, 1948
BsmAI GTCTC 3 cut(s) 228, 1380, 1417
BsmFI GGGAC 4 cut(s) 68, 584, 1841, 1952
BsmI GAATGC 3 cut(s) 318, 657, 2217
BsnI GGCC 6 cut(s) 664, 670, 874, 1214, 1262, 1503
Bsp143I GATC 6 cut(s) 1039, 1222, 1447, 1855, 2049, 2365
Bsp19I CCATGG 1 cut(s) 665
BspACI CCGC 3 cut(s) 28, 723, 1400
BspANI GGCC 6 cut(s) 664, 670, 874, 1214, 1262, 1503
BspCNI CTCAG 1 cut(s) 1249
BspLI GGNNCC 3 cut(s) 856, 1213, 1940
BspMAI CTGCAG 1 cut(s) 472
BspPI GGATC 4 cut(s) 1034, 1863, 2057, 2360
BspT107I GGYRCC 1 cut(s) 854
BspTI CTTAAG 1 cut(s) 1364
BsrBI CCGCTC 1 cut(s) 723
BsrFI RCCGGY 1 cut(s) 165
BsrI ACTGG 5 cut(s) 403, 842, 1152, 1294, 1307
BssAI RCCGGY 1 cut(s) 165
BssECI CCNNGG 6 cut(s) 148, 484, 559, 665, 911, 1504
BssMI GATC 6 cut(s) 1039, 1222, 1447, 1855, 2049, 2365
BssSI CACGAG 1 cut(s) 1803
BssT1I CCWWGG 2 cut(s) 665, 1504
Bst2BI CACGAG 1 cut(s) 1803
Bst2UI CCWGG 4 cut(s) 486, 561, 912, 1949
Bst4CI ACNGT 3 cut(s) 214, 348, 699
Bst6I CTCTTC 3 cut(s) 29, 38, 2313
BstAFI CTTAAG 1 cut(s) 1364
BstC8I GCNNGC 3 cut(s) 657, 2240, 2328
BstDEI CTNAG 4 cut(s) 639, 1257, 1419, 2319
BstDSI CCRYGG 1 cut(s) 665
BstEII GGTNACC 1 cut(s) 1156
BstF5I GGATG 6 cut(s) 94, 427, 750, 971, 1402, 2038
BstKTI GATC 6 cut(s) 1042, 1225, 1450, 1858, 2052, 2368
BstMAI GTCTC 3 cut(s) 228, 1380, 1417
BstMBI GATC 6 cut(s) 1039, 1222, 1447, 1855, 2049, 2365
BstMWI GCNNNNNNNGC 6 cut(s) 159, 299, 661, 1325, 1625, 2273
BstNI CCWGG 4 cut(s) 486, 561, 912, 1949
BstNSI RCATGY 5 cut(s) 659, 1198, 1711, 1990, 2132
BstPI GGTNACC 1 cut(s) 1156
BstSCI CCNGG 9 cut(s) 148, 417, 484, 559, 755, 910, 1428, 1864, 1947
BstSFI CTRYAG 2 cut(s) 468, 930
BstV1I GCAGC 3 cut(s) 1478, 1652, 2335
BstV2I GAAGAC 3 cut(s) 807, 1385, 2157
BstX2I RGATCY 3 cut(s) 1039, 1222, 1855
BstYI RGATCY 3 cut(s) 1039, 1222, 1855
BsuI GTATCC 1 cut(s) 388
BsuRI GGCC 6 cut(s) 664, 670, 874, 1214, 1262, 1503
BtgI CCRYGG 1 cut(s) 665
BtsCI GGATG 6 cut(s) 94, 427, 750, 971, 1402, 2038
BtsI GCAGTG 1 cut(s) 1525
BtsIMutI CAGTG 5 cut(s) 219, 803, 1145, 1457, 1525
Cac8I GCNNGC 3 cut(s) 657, 2240, 2328
Cfr10I RCCGGY 1 cut(s) 165
Cfr13I GGNCC 6 cut(s) 352, 662, 1212, 1297, 1939, 2257
Csp6I GTAC 3 cut(s) 169, 769, 1310
CspAI ACCGGT 1 cut(s) 165
CspCI CAANNNNNGTGG 2 cut(s) 195, 230
CviQI GTAC 3 cut(s) 169, 769, 1310
DdeI CTNAG 4 cut(s) 639, 1257, 1419, 2319
DpnI GATC 6 cut(s) 1041, 1224, 1449, 1857, 2051, 2367
DpnII GATC 6 cut(s) 1039, 1222, 1447, 1855, 2049, 2365
DraI TTTAAA 1 cut(s) 1764
EaeI YGGCCR 1 cut(s) 1501
Eam1104I CTCTTC 3 cut(s) 29, 38, 2313
EarI CTCTTC 3 cut(s) 29, 38, 2313
EciI GGCGGA 1 cut(s) 17
Eco130I CCWWGG 2 cut(s) 665, 1504
Eco147I AGGCCT 1 cut(s) 874
Eco47I GGWCC 4 cut(s) 352, 1297, 1939, 2257
Eco57I CTGAAG 6 cut(s) 104, 127, 749, 1267, 1566, 1619
Eco91I GGTNACC 1 cut(s) 1156
EcoO65I GGTNACC 1 cut(s) 1156
EcoRI GAATTC 1 cut(s) 1601
EcoRII CCWGG 4 cut(s) 484, 559, 910, 1947
EcoT14I CCWWGG 2 cut(s) 665, 1504
EcoT22I ATGCAT 3 cut(s) 657, 2106, 2219
ErhI CCWWGG 2 cut(s) 665, 1504
FalI AAGNNNNNCTT 2 cut(s) 2214, 2246
FaqI GGGAC 4 cut(s) 68, 584, 1841, 1952
FblI GTMKAC 2 cut(s) 332, 624
Fnu4HI GCNGC 3 cut(s) 1467, 1666, 2324
FokI GGATG 6 cut(s) 101, 434, 757, 978, 1409, 2045
Fsp4HI GCNGC 3 cut(s) 1467, 1666, 2324
FspBI CTAG 6 cut(s) 159, 521, 1575, 1880, 2183, 2394
GluI GCNGC 3 cut(s) 1467, 1666, 2324
GsuI CTGGAG 1 cut(s) 279
HaeIII GGCC 6 cut(s) 664, 670, 874, 1214, 1262, 1503
HapII CCGG 7 cut(s) 150, 166, 418, 756, 1215, 1430, 1866
HindIII AAGCTT 2 cut(s) 436, 1720
HpaII CCGG 7 cut(s) 150, 166, 418, 756, 1215, 1430, 1866
HphI GGTGA 3 cut(s) 110, 1983, 2309
Hpy166II GTNNAC 4 cut(s) 333, 625, 977, 1204
Hpy188I TCNGA 8 cut(s) 65, 594, 948, 1486, 1909, 1973, 2032, 2342
Hpy8I GTNNAC 4 cut(s) 333, 625, 977, 1204
Hpy99I CGWCG 1 cut(s) 2282
HpyAV CCTTC 7 cut(s) 79, 742, 1004, 1015, 1886, 2248, 2380
HpyCH4III ACNGT 3 cut(s) 214, 348, 699
HpyCH4IV ACGT 1 cut(s) 171
HpyF10VI GCNNNNNNNGC 6 cut(s) 159, 299, 661, 1325, 1625, 2273
HpyF3I CTNAG 4 cut(s) 639, 1257, 1419, 2319
HpySE526I ACGT 1 cut(s) 171
Kzo9I GATC 6 cut(s) 1039, 1222, 1447, 1855, 2049, 2365
LmnI GCTCC 2 cut(s) 595, 720
Lsp1109I GCAGC 3 cut(s) 1478, 1652, 2335
LweI GCATC 6 cut(s) 775, 1387, 1477, 1873, 2091, 2204
MaeI CTAG 6 cut(s) 159, 521, 1575, 1880, 2183, 2394
MaeII ACGT 1 cut(s) 171
MaeIII GTNAC 6 cut(s) 546, 759, 833, 1156, 1755, 1989
MalI GATC 6 cut(s) 1041, 1224, 1449, 1857, 2051, 2367
MbiI CCGCTC 1 cut(s) 723
MboI GATC 6 cut(s) 1039, 1222, 1447, 1855, 2049, 2365
MfeI CAATTG 1 cut(s) 1162
MflI RGATCY 3 cut(s) 1039, 1222, 1855
MlsI TGGCCA 1 cut(s) 1503
MluNI TGGCCA 1 cut(s) 1503
MlyI GAGTC 2 cut(s) 1721, 1823
MmeI TCCRAC 2 cut(s) 920, 1951
MnlI CCTC 9 cut(s) 41, 265, 1252, 1368, 1396, 1405, 1746, 1833, 2073
Mox20I TGGCCA 1 cut(s) 1503
Mph1103I ATGCAT 3 cut(s) 657, 2106, 2219
MroXI GAANNNNTTC 1 cut(s) 2347
MscI TGGCCA 1 cut(s) 1503
MseI TTAA 6 cut(s) 407, 533, 885, 1365, 1452, 1763
MslI CAYNNNNRTG 1 cut(s) 852
Msp20I TGGCCA 1 cut(s) 1503
MspCI CTTAAG 1 cut(s) 1364
MspI CCGG 7 cut(s) 150, 166, 418, 756, 1215, 1430, 1866
MspR9I CCNGG 9 cut(s) 150, 419, 486, 561, 757, 912, 1430, 1866, 1949
MunI CAATTG 1 cut(s) 1162
Mva1269I GAATGC 3 cut(s) 318, 657, 2217
MvaI CCWGG 4 cut(s) 486, 561, 912, 1949
MwoI GCNNNNNNNGC 6 cut(s) 159, 299, 661, 1325, 1625, 2273
NciI CCSGG 5 cut(s) 150, 419, 757, 1430, 1866
NcoI CCATGG 1 cut(s) 665
NdeII GATC 6 cut(s) 1039, 1222, 1447, 1855, 2049, 2365
NlaIV GGNNCC 3 cut(s) 856, 1213, 1940
NmeAIII GCCGAG 1 cut(s) 1912
NmuCI GTSAC 4 cut(s) 546, 833, 1755, 1989
NsiI ATGCAT 3 cut(s) 657, 2106, 2219
NspI RCATGY 5 cut(s) 659, 1198, 1711, 1990, 2132
PaeI GCATGC 1 cut(s) 659
PceI AGGCCT 1 cut(s) 874
PciI ACATGT 3 cut(s) 1194, 1986, 2128
PctI GAATGC 3 cut(s) 318, 657, 2217
PdmI GAANNNNTTC 1 cut(s) 2347
PfeI GAWTC 8 cut(s) 155, 595, 752, 943, 1593, 1645, 2174, 2384
PflFI GACNNNGTC 1 cut(s) 2156
PflMI CCANNNNNTGG 3 cut(s) 66, 391, 1948
PfoI TCCNGGA 2 cut(s) 417, 1947
PinAI ACCGGT 1 cut(s) 165
PkrI GCNGC 3 cut(s) 1468, 1667, 2325
PleI GAGTC 2 cut(s) 1721, 1823
PpsI GAGTC 2 cut(s) 1721, 1823
PscI ACATGT 3 cut(s) 1194, 1986, 2128
PshBI ATTAAT 1 cut(s) 885
PsiI TTATAA 2 cut(s) 306, 1527
Psp6I CCWGG 4 cut(s) 484, 559, 910, 1947
PspEI GGTNACC 1 cut(s) 1156
PspGI CCWGG 4 cut(s) 484, 559, 910, 1947
PspN4I GGNNCC 3 cut(s) 856, 1213, 1940
PspPI GGNCC 6 cut(s) 352, 662, 1212, 1297, 1939, 2257
PstI CTGCAG 1 cut(s) 472
PsuI RGATCY 3 cut(s) 1039, 1222, 1855
PsyI GACNNNGTC 1 cut(s) 2156
RsaI GTAC 3 cut(s) 170, 770, 1311
RsaNI GTAC 3 cut(s) 169, 769, 1310
RseI CAYNNNNRTG 1 cut(s) 852
SaqAI TTAA 6 cut(s) 407, 533, 885, 1365, 1452, 1763
SatI GCNGC 3 cut(s) 1467, 1666, 2324
Sau3AI GATC 6 cut(s) 1039, 1222, 1447, 1855, 2049, 2365
Sau96I GGNCC 6 cut(s) 352, 662, 1212, 1297, 1939, 2257
ScaI AGTACT 1 cut(s) 1311
SchI GAGTC 2 cut(s) 1721, 1823
ScrFI CCNGG 9 cut(s) 150, 419, 486, 561, 757, 912, 1430, 1866, 1949
SfaNI GCATC 6 cut(s) 775, 1387, 1477, 1873, 2091, 2204
SfcI CTRYAG 2 cut(s) 468, 930
SinI GGWCC 4 cut(s) 352, 1297, 1939, 2257
SmiMI CAYNNNNRTG 1 cut(s) 852
SmlI CTYRAG 4 cut(s) 1364, 1409, 1831, 2209
SmoI CTYRAG 4 cut(s) 1364, 1409, 1831, 2209
SphI GCATGC 1 cut(s) 659
SseBI AGGCCT 1 cut(s) 874
SsiI CCGC 3 cut(s) 28, 723, 1400
SspI AATATT 3 cut(s) 617, 883, 1929
SspMI CTAG 6 cut(s) 159, 521, 1575, 1880, 2183, 2394
StuI AGGCCT 1 cut(s) 874
StyD4I CCNGG 9 cut(s) 148, 417, 484, 559, 755, 910, 1428, 1864, 1947
StyI CCWWGG 2 cut(s) 665, 1504
TaaI ACNGT 3 cut(s) 214, 348, 699
TaiI ACGT 1 cut(s) 174
TaqI TCGA 4 cut(s) 1052, 1446, 1596, 2428
TaqII GACCGA 2 cut(s) 340, 2274
TatI WGTACW 1 cut(s) 1309
TfiI GAWTC 8 cut(s) 155, 595, 752, 943, 1593, 1645, 2174, 2384
Tru1I TTAA 6 cut(s) 407, 533, 885, 1365, 1452, 1763
Tru9I TTAA 6 cut(s) 407, 533, 885, 1365, 1452, 1763
TscAI CASTG 5 cut(s) 219, 803, 1152, 1464, 1525
TseFI GTSAC 4 cut(s) 546, 833, 1755, 1989
TseI GCWGC 3 cut(s) 1466, 1665, 2323
Tsp45I GTSAC 4 cut(s) 546, 833, 1755, 1989
TspRI CASTG 5 cut(s) 219, 803, 1152, 1464, 1525
Tth111I GACNNNGTC 1 cut(s) 2156
Van91I CCANNNNNTGG 3 cut(s) 66, 391, 1948
Vha464I CTTAAG 1 cut(s) 1364
VpaK11BI GGWCC 4 cut(s) 352, 1297, 1939, 2257
VspI ATTAAT 1 cut(s) 885
XapI RAATTY 3 cut(s) 1245, 1601, 1902
XbaI TCTAGA 3 cut(s) 520, 1574, 1879
XceI RCATGY 5 cut(s) 659, 1198, 1711, 1990, 2132
XcmI CCANNNNNNNNNTGG 2 cut(s) 170, 1511
XmiI GTMKAC 2 cut(s) 332, 624
XmnI GAANNNNTTC 1 cut(s) 2347
XspI CTAG 6 cut(s) 159, 521, 1575, 1880, 2183, 2394
ZrmI AGTACT 1 cut(s) 1311
Zsp2I ATGCAT 3 cut(s) 657, 2106, 2219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.