Prupe.4G032200_v2.0.a1

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000112
Physical Location & Seq
Forward (+)
1495196 .. 1498810
3615 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G032200.1

Sequence Viewer

Length: 2376 bp
ATGTTTTTAAATACTTTGCTTGTCTTCTTCTCCCTCTTCCCATTTTGCACATCCATTGACACCATAGCAGTGAACCAGCAGGTGAAAGATGGTGACTTTATAGTGTCCAAAGAAAACAACTTTGAATTAGGCTTCTTCAGCCCCGGCAACTCGAGCTCTCGCTATGTTGGGATTTGGTATGCTAATAAGTCTGAAAAGGCAGTGGTTTGGGTTGCAAACAGAAACAACCCCATCAATGATACCTCCGGTGTTCTCACAATCAACAGGTACGGAAAATTGGTTCTTTATGCTCACAATACTGAGAATCTTTCTATTTGGTCCACAAATGTGTCGGTCCAAACCACAAGCAGTGTAGCTCAGCTTTTAGATACAGGAAATTTAGTGCTTTTCCAGGATAGTAAAAGTGAAAGCTTTATATGGCAAAGTTTTGATTATCCTACAGATACTCTACTTTCCGGAATGAAAGTTGGGTTGAATTGGAAAATTGGGTTGGAATGGGTCTTAACATCTTGGAAGTCCCAAAATGACCCTGGAACTGGTAACTATAGCTTGAGGCTTTATTCAAACCAGACTGCTACGCCCCAATATTTTCTGTACAAGGGTTTGACCAAATATTGGAGATGTGATCCAGGGCCATGGCCCGTTTTTGTGAGTAATAATGAAGAAATGTATTATTTCTTTTTAACTGATAACACCTCTGTAATTAGATCCGTGTTGACGGATTATGGGGTAAATGAACAACTGAGATGGAATGATGCTGTTAATCAGTGGGAGGTACTCTTTGCTGCACCAAAATACCGGTGTGACCGATATGGACAGTGTGGTGCCAACAGCAAATGTAGCCCTGACAATATTAATATGTTTGAATGTGAGTGCCTACCGGGGTATGAACCAAAATCTTCGAATCATTGGAACCGGAGAGATGGGTCAGATGGGTGCGTGAGCAAGCGAATTGGGGTGTCAAACTGTGGGGATGGGGATGGGTTTGTGAAGGTGGCACGGGTGAAAGATCCAGACACATCGAAAGCAGCACAATTGTTGCCAAATACTAGTGCCAATGAGTGTGAGCAGGTGTGCTTAAGCAATTGTTTCTGCACGGCATATATGAGCATAGAATGGGAAGGCCGCATTAATTGCTTGGCATGGTATGGTCAGTTGTGGGACATTTTAATACACACGGAGCTAGGGCGAGATCTATATGTTCGTGTGGACAAAATGGAATTAGCTGAGAATACCAGAAAATTGAAAGGTTTCCTCAAAAGTAGGGGCTTGCTGGCTATTCCAATGGCGGCGGTTGCATTGGTACTAATCATTGGGTTTGCCTGCTGGTGGCTTAAGAAAAAGAGGAAGATGAAAGAAACTAGGAGACACCCAGAGCTGCAATTTTTCCATCTGAGCGCAATAATAGTAGCCACAGGCAACTTCTCTCCTGTCAATAAACTTGGCCAAGGTGGTTTTGGCACTGTTTATAAGGGTCTGTTAGCAAATGATCAGAAGATTGCTGTAAAAAGATTGTCCAAAACTTCAGGACAAGGAATTGAAGAATTCAAAAATGAAGTTGCGCTGATAGCCAGACTTCAACACAGGAATCTTGTGAAACTTTTAGGCTGTTGTATAAAGGGAGAAGAAAGGATGTTAGTCCTGGAATACTTGCCGAACAAAAGCTTGGACTCCTTTCTTTTTGATCACACAAGAAGTTCCTTGTTGGACTGGAAAAAGCGCTTTGAAATTATCAACGGGGTTGCTCGTGGGATTCTGTATCTTCACCAGGATTCAAGACTTAGAATTATTCATAGGGATCTAAAAACCAGCAATGTTCTTCTAGATGCTGAGATGAACCCAAAAATATCAGATTTTGGCATGGCAAGAATATTCCATGGGGACCAACTGCAGGATAAAACCAACAGAGTTGTTGGAACATATGGATATATGTCACCTGAGTATGCAGTGTTTGGAAGATATTCAACTAAATCAGATGTCTTTAGTTTTGGGATCATATTATTGGAGATTGTGAGTGGCAAGAAAAACAATGGCTCTTATCAAGAGGACCATTCCATGAACTTGATAGGACATGTTTGGCAGCTGTGGAGAGAAGATAGAGCCTTAGAAATTGTGGATTCATCACTAGAGTCATATCATTCTGATGAAGTCTTGAGATGCATTCAAGTCGGGCTCTTGTGCGTGCAAGAAGATTCAGAGGACCGACCAACCATGTCAGCCGTTTTATTTATGTTGAGTGGTGAAGCATCTCTACCATCTCCTCAGCAGCCTGCATTTGTCTTCAGAAAAAGTTCCGGCGGTGGTGGTTATCTATCAAGTCCACAAGGATTATATTCTGTTAATGACTTGACAATAACTAAACTTGACGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

792

Amino Acids

89.49

Weight (kDa)

7.96

Isoelectric Point (pI)

39.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1470
AarI CACCTGC 2 cut(s) 70, 1060
Acc36I ACCTGC 2 cut(s) 70, 1060
AccB1I GGYRCC 1 cut(s) 824
AccB7I CCANNNNNTGG 1 cut(s) 615
AccIII TCCGGA 1 cut(s) 455
AciI CCGC 4 cut(s) 1126, 1289, 1292, 2298
AclWI GGATC 5 cut(s) 620, 702, 1004, 1806, 2000
AcoI YGGCCR 1 cut(s) 1444
AcsI RAATTY 2 cut(s) 376, 1544
AcuI CTGAAG 3 cut(s) 121, 1509, 2266
AfaI GTAC 4 cut(s) 269, 596, 777, 1305
AfeI AGCGCT 1 cut(s) 1721
AfiI CCNNNNNNNGG 4 cut(s) 536, 615, 1329, 1891
AflII CTTAAG 2 cut(s) 1078, 1334
AflIII ACRYGT 1 cut(s) 2071
AgeI ACCGGT 1 cut(s) 798
AhlI ACTAGT 1 cut(s) 1049
AjnI CCWGG 5 cut(s) 390, 529, 628, 1641, 1767
AjuI GAANNNNNNNTTGG 4 cut(s) 473, 505, 1649, 1681
AleI CACNNNNGTG 1 cut(s) 326
AloI GAACNNNNNNTCC 2 cut(s) 264, 296
Alw21I GWGCWC 1 cut(s) 158
Alw26I GTCTC 1 cut(s) 1360
AlwI GGATC 5 cut(s) 620, 702, 1004, 1806, 2000
Ama87I CYCGRG 1 cut(s) 151
Aor13HI TCCGGA 1 cut(s) 455
Aor51HI AGCGCT 1 cut(s) 1721
AoxI GGCC 4 cut(s) 632, 638, 1123, 1444
ApeKI GCWGC 5 cut(s) 785, 1028, 1378, 2080, 2266
ApoI RAATTY 2 cut(s) 376, 1544
AseI ATTAAT 2 cut(s) 855, 1131
AsiGI ACCGGT 1 cut(s) 798
Asp700I GAANNNNTTC 3 cut(s) 1250, 1960, 2290
AspLEI GCGC 3 cut(s) 1400, 1564, 1722
AspS9I GGNCC 7 cut(s) 318, 334, 632, 639, 1882, 2047, 2200
AsuC2I CCSGG 2 cut(s) 144, 882
AsuHPI GGTGA 6 cut(s) 94, 104, 1015, 1757, 1926, 2252
AsuII TTCGAA 1 cut(s) 902
AvaI CYCGRG 1 cut(s) 151
AvaII GGWCC 5 cut(s) 318, 334, 1882, 2047, 2200
BalI TGGCCA 1 cut(s) 1446
BanI GGYRCC 1 cut(s) 824
BanII GRGCYC 2 cut(s) 158, 2175
BarI GAAGNNNNNNTAC 2 cut(s) 2235, 2267
BauI CACGAG 1 cut(s) 1746
BbsI GAAGAC 2 cut(s) 16, 2272
Bbv12I GWGCWC 1 cut(s) 158
BbvCI CCTCAGC 1 cut(s) 2262
BbvI GCAGC 5 cut(s) 772, 1040, 1365, 2092, 2278
BccI CCATC 9 cut(s) 83, 239, 741, 917, 926, 968, 974, 1398, 2263
BceAI ACGGC 2 cut(s) 1113, 2204
BcgI CGANNNNNNTGC 2 cut(s) 2149, 2183
BciT130I CCWGG 5 cut(s) 392, 531, 630, 1643, 1769
BclI TGATCA 2 cut(s) 1489, 1684
BcnI CCSGG 2 cut(s) 144, 882
BcoDI GTCTC 1 cut(s) 1360
BcuI ACTAGT 1 cut(s) 1049
BfaI CTAG 5 cut(s) 1050, 1184, 1362, 1823, 2126
BfmI CTRYAG 3 cut(s) 438, 544, 1889
BfoI RGCGCY 1 cut(s) 1723
BfrI CTTAAG 2 cut(s) 1078, 1334
BfuAI ACCTGC 2 cut(s) 70, 1060
BglII AGATCT 1 cut(s) 1192
BisI GCNGC 7 cut(s) 786, 1029, 1126, 1290, 1379, 2081, 2267
BlpI GCTNAGC 1 cut(s) 357
BlsI GCNGC 7 cut(s) 787, 1030, 1127, 1291, 1380, 2082, 2268
Bme1390I CCNGG 7 cut(s) 144, 392, 531, 630, 882, 1643, 1769
Bme18I GGWCC 5 cut(s) 318, 334, 1882, 2047, 2200
BmeT110I CYCGRG 1 cut(s) 151
BmgT120I GGNCC 7 cut(s) 318, 334, 632, 639, 1882, 2047, 2200
BmiI GGNNCC 3 cut(s) 826, 914, 1883
BmrFI CCNGG 7 cut(s) 144, 392, 531, 630, 882, 1643, 1769
BmsI GCATC 4 cut(s) 745, 1816, 2147, 2255
BpiI GAAGAC 2 cut(s) 16, 2272
Bpu10I CCTNAGC 1 cut(s) 2262
Bpu1102I GCTNAGC 1 cut(s) 357
Bpu14I TTCGAA 1 cut(s) 902
BpuEI CTTGAG 2 cut(s) 571, 2173
BpuMI CCSGG 2 cut(s) 144, 882
BsaJI CCNNGG 7 cut(s) 142, 529, 629, 635, 881, 1447, 1876
BsaWI WCCGGW 4 cut(s) 245, 455, 798, 915
Bsc4I CCNNNNNNNGG 4 cut(s) 536, 615, 1329, 1891
Bse118I RCCGGY 1 cut(s) 798
Bse1I ACTGG 2 cut(s) 541, 1715
Bse3DI GCAATG 1 cut(s) 1819
BseAI TCCGGA 1 cut(s) 455
BseBI CCWGG 5 cut(s) 392, 531, 630, 1643, 1769
BseDI CCNNGG 7 cut(s) 142, 529, 629, 635, 881, 1447, 1876
BseGI GGATG 4 cut(s) 50, 979, 985, 1638
BseLI CCNNNNNNNGG 4 cut(s) 536, 615, 1329, 1891
BseMI GCAATG 1 cut(s) 1819
BseMII CTCAG 8 cut(s) 291, 371, 734, 1218, 1385, 1821, 1929, 2276
BseNI ACTGG 2 cut(s) 541, 1715
BseRI GAGGAG 1 cut(s) 2250
BseXI GCAGC 5 cut(s) 772, 1040, 1365, 2092, 2278
BsgI GTGCAG 2 cut(s) 771, 1078
BshFI GGCC 4 cut(s) 634, 640, 1125, 1446
BshNI GGYRCC 1 cut(s) 824
BshTI ACCGGT 1 cut(s) 798
BsiHKAI GWGCWC 1 cut(s) 158
BsiHKCI CYCGRG 1 cut(s) 151
BsiSI CCGG 7 cut(s) 144, 246, 456, 799, 881, 916, 2295
BslFI GGGAC 3 cut(s) 502, 1175, 1895
BslI CCNNNNNNNGG 4 cut(s) 536, 615, 1329, 1891
BsmAI GTCTC 1 cut(s) 1360
BsmFI GGGAC 3 cut(s) 502, 1175, 1895
BsmI GAATGC 1 cut(s) 2160
BsnI GGCC 4 cut(s) 634, 640, 1125, 1446
BsoBI CYCGRG 1 cut(s) 151
Bsp119I TTCGAA 1 cut(s) 902
Bsp1286I GDGCHC 2 cut(s) 158, 2175
Bsp13I TCCGGA 1 cut(s) 455
Bsp1407I TGTACA 1 cut(s) 594
Bsp143I GATC 8 cut(s) 625, 707, 1009, 1192, 1489, 1684, 1798, 1992
Bsp1720I GCTNAGC 1 cut(s) 357
Bsp19I CCATGG 2 cut(s) 635, 1876
BspACI CCGC 4 cut(s) 1126, 1289, 1292, 2298
BspANI GGCC 4 cut(s) 634, 640, 1125, 1446
BspCNI CTCAG 8 cut(s) 292, 370, 735, 1219, 1386, 1822, 1930, 2275
BspEI TCCGGA 1 cut(s) 455
BspLI GGNNCC 3 cut(s) 826, 914, 1883
BspMAI CTGCAG 1 cut(s) 1893
BspMI ACCTGC 2 cut(s) 70, 1060
BspPI GGATC 5 cut(s) 620, 702, 1004, 1806, 2000
BspT104I TTCGAA 1 cut(s) 902
BspT107I GGYRCC 1 cut(s) 824
BspTI CTTAAG 2 cut(s) 1078, 1334
BsrDI GCAATG 1 cut(s) 1819
BsrFI RCCGGY 1 cut(s) 798
BsrGI TGTACA 1 cut(s) 594
BsrI ACTGG 2 cut(s) 541, 1715
BssAI RCCGGY 1 cut(s) 798
BssECI CCNNGG 7 cut(s) 142, 529, 629, 635, 881, 1447, 1876
BssMI GATC 8 cut(s) 625, 707, 1009, 1192, 1489, 1684, 1798, 1992
BssSI CACGAG 1 cut(s) 1746
BssT1I CCWWGG 3 cut(s) 635, 1447, 1876
Bst2BI CACGAG 1 cut(s) 1746
Bst2UI CCWGG 5 cut(s) 392, 531, 630, 1643, 1769
Bst4CI ACNGT 3 cut(s) 819, 968, 1465
Bst6I CTCTTC 1 cut(s) 41
BstAFI CTTAAG 2 cut(s) 1078, 1334
BstAPI GCANNNNNTGC 1 cut(s) 1134
BstAUI TGTACA 1 cut(s) 594
BstBI TTCGAA 1 cut(s) 902
BstC8I GCNNGC 6 cut(s) 947, 1271, 1275, 1324, 2183, 2271
BstDSI CCRYGG 2 cut(s) 635, 1876
BstF5I GGATG 4 cut(s) 50, 979, 985, 1638
BstH2I RGCGCY 1 cut(s) 1723
BstHHI GCGC 3 cut(s) 1400, 1564, 1722
BstKTI GATC 8 cut(s) 628, 710, 1012, 1195, 1492, 1687, 1801, 1995
BstMAI GTCTC 1 cut(s) 1360
BstMBI GATC 8 cut(s) 625, 707, 1009, 1192, 1489, 1684, 1798, 1992
BstMWI GCNNNNNNNGC 6 cut(s) 138, 153, 840, 1134, 1295, 1568
BstNI CCWGG 5 cut(s) 392, 531, 630, 1643, 1769
BstNSI RCATGY 1 cut(s) 2075
BstSCI CCNGG 7 cut(s) 142, 390, 529, 628, 880, 1641, 1767
BstSFI CTRYAG 3 cut(s) 438, 544, 1889
BstV1I GCAGC 5 cut(s) 772, 1040, 1365, 2092, 2278
BstV2I GAAGAC 2 cut(s) 16, 2272
BstX2I RGATCY 4 cut(s) 707, 1009, 1192, 1798
BstXI CCANNNNNNTGG 1 cut(s) 636
BstYI RGATCY 4 cut(s) 707, 1009, 1192, 1798
BsuRI GGCC 4 cut(s) 634, 640, 1125, 1446
BtgI CCRYGG 2 cut(s) 635, 1876
BtsCI GGATG 4 cut(s) 50, 979, 985, 1638
BtsI GCAGTG 4 cut(s) 75, 207, 355, 1953
BtsIMutI CAGTG 7 cut(s) 75, 207, 355, 773, 824, 1461, 1953
BveI ACCTGC 2 cut(s) 70, 1060
Cac8I GCNNGC 6 cut(s) 947, 1271, 1275, 1324, 2183, 2271
CfoI GCGC 3 cut(s) 1400, 1564, 1722
Cfr10I RCCGGY 1 cut(s) 798
Cfr13I GGNCC 7 cut(s) 318, 334, 632, 639, 1882, 2047, 2200
Csp6I GTAC 4 cut(s) 268, 595, 776, 1304
CspAI ACCGGT 1 cut(s) 798
CviAII CATG 7 cut(s) 636, 1143, 1861, 1877, 2056, 2072, 2212
CviQI GTAC 4 cut(s) 268, 595, 776, 1304
DpnI GATC 8 cut(s) 627, 709, 1011, 1194, 1491, 1686, 1800, 1994
DpnII GATC 8 cut(s) 625, 707, 1009, 1192, 1489, 1684, 1798, 1992
DraI TTTAAA 1 cut(s) 9
EaeI YGGCCR 1 cut(s) 1444
Eam1104I CTCTTC 1 cut(s) 41
EarI CTCTTC 1 cut(s) 41
Ecl136II GAGCTC 1 cut(s) 156
Eco130I CCWWGG 3 cut(s) 635, 1447, 1876
Eco24I GRGCYC 2 cut(s) 158, 2175
Eco47I GGWCC 5 cut(s) 318, 334, 1882, 2047, 2200
Eco47III AGCGCT 1 cut(s) 1721
Eco53kI GAGCTC 1 cut(s) 156
Eco57I CTGAAG 3 cut(s) 121, 1509, 2266
Eco88I CYCGRG 1 cut(s) 151
EcoICRI GAGCTC 1 cut(s) 156
EcoRI GAATTC 1 cut(s) 1544
EcoRII CCWGG 5 cut(s) 390, 529, 628, 1641, 1767
EcoT14I CCWWGG 3 cut(s) 635, 1447, 1876
EcoT22I ATGCAT 1 cut(s) 2162
EcoT38I GRGCYC 2 cut(s) 158, 2175
ErhI CCWWGG 3 cut(s) 635, 1447, 1876
FaeI CATG 7 cut(s) 639, 1146, 1864, 1880, 2059, 2075, 2215
FaqI GGGAC 3 cut(s) 502, 1175, 1895
FatI CATG 7 cut(s) 635, 1142, 1860, 1876, 2055, 2071, 2211
FauNDI CATATG 1 cut(s) 1921
FbaI TGATCA 2 cut(s) 1489, 1684
Fnu4HI GCNGC 7 cut(s) 786, 1029, 1126, 1290, 1379, 2081, 2267
FokI GGATG 4 cut(s) 37, 986, 992, 1645
FriOI GRGCYC 2 cut(s) 158, 2175
Fsp4HI GCNGC 7 cut(s) 786, 1029, 1126, 1290, 1379, 2081, 2267
FspBI CTAG 5 cut(s) 1050, 1184, 1362, 1823, 2126
GlaI GCGC 3 cut(s) 1399, 1563, 1721
GluI GCNGC 7 cut(s) 786, 1029, 1126, 1290, 1379, 2081, 2267
HaeII RGCGCY 1 cut(s) 1723
HaeIII GGCC 4 cut(s) 634, 640, 1125, 1446
HapII CCGG 7 cut(s) 144, 246, 456, 799, 881, 916, 2295
HhaI GCGC 3 cut(s) 1400, 1564, 1722
Hin1II CATG 7 cut(s) 639, 1146, 1864, 1880, 2059, 2075, 2215
Hin6I GCGC 3 cut(s) 1398, 1562, 1720
HinP1I GCGC 3 cut(s) 1398, 1562, 1720
HincII GTYRAC 1 cut(s) 717
HindII GTYRAC 1 cut(s) 717
HindIII AAGCTT 2 cut(s) 409, 1663
HinfI GANTC 9 cut(s) 304, 904, 1588, 1670, 1753, 1772, 2117, 2129, 2192
HpaII CCGG 7 cut(s) 144, 246, 456, 799, 881, 916, 2295
HphI GGTGA 6 cut(s) 94, 104, 1015, 1757, 1926, 2252
Hpy166II GTNNAC 5 cut(s) 73, 321, 717, 1210, 2321
Hpy188I TCNGA 9 cut(s) 193, 931, 1395, 1494, 1852, 1975, 2143, 2197, 2285
Hpy188III TCNNGA 7 cut(s) 456, 1013, 1527, 1776, 1823, 2042, 2152
Hpy8I GTNNAC 5 cut(s) 73, 321, 717, 1210, 2321
HpyAV CCTTC 2 cut(s) 985, 1115
HpyCH4III ACNGT 3 cut(s) 819, 968, 1465
HpyF10VI GCNNNNNNNGC 6 cut(s) 138, 153, 840, 1134, 1295, 1568
Hsp92II CATG 7 cut(s) 639, 1146, 1864, 1880, 2059, 2075, 2215
HspAI GCGC 3 cut(s) 1398, 1562, 1720
Kpn2I TCCGGA 1 cut(s) 455
Ksp22I TGATCA 2 cut(s) 1489, 1684
Kzo9I GATC 8 cut(s) 625, 707, 1009, 1192, 1489, 1684, 1798, 1992
LmnI GCTCC 1 cut(s) 1180
Lsp1109I GCAGC 5 cut(s) 772, 1040, 1365, 2092, 2278
LweI GCATC 4 cut(s) 745, 1816, 2147, 2255
MaeI CTAG 5 cut(s) 1050, 1184, 1362, 1823, 2126
MaeIII GTNAC 4 cut(s) 92, 539, 803, 1932
MalI GATC 8 cut(s) 627, 709, 1011, 1194, 1491, 1686, 1800, 1994
MboI GATC 8 cut(s) 625, 707, 1009, 1192, 1489, 1684, 1798, 1992
MfeI CAATTG 2 cut(s) 1034, 1084
MflI RGATCY 4 cut(s) 707, 1009, 1192, 1798
MhlI GDGCHC 2 cut(s) 158, 2175
MlsI TGGCCA 1 cut(s) 1446
MluNI TGGCCA 1 cut(s) 1446
MlyI GAGTC 2 cut(s) 1664, 2138
MmeI TCCRAC 3 cut(s) 471, 1686, 1894
Mox20I TGGCCA 1 cut(s) 1446
Mph1103I ATGCAT 1 cut(s) 2162
MroI TCCGGA 1 cut(s) 455
MroXI GAANNNNTTC 3 cut(s) 1250, 1960, 2290
MscI TGGCCA 1 cut(s) 1446
MslI CAYNNNNRTG 3 cut(s) 68, 326, 2142
Msp20I TGGCCA 1 cut(s) 1446
MspA1I CMGCKG 1 cut(s) 2083
MspCI CTTAAG 2 cut(s) 1078, 1334
MspI CCGG 7 cut(s) 144, 246, 456, 799, 881, 916, 2295
MspR9I CCNGG 7 cut(s) 144, 392, 531, 630, 882, 1643, 1769
MunI CAATTG 2 cut(s) 1034, 1084
Mva1269I GAATGC 1 cut(s) 2160
MvaI CCWGG 5 cut(s) 392, 531, 630, 1643, 1769
MwoI GCNNNNNNNGC 6 cut(s) 138, 153, 840, 1134, 1295, 1568
NciI CCSGG 2 cut(s) 144, 882
NcoI CCATGG 2 cut(s) 635, 1876
NdeI CATATG 1 cut(s) 1921
NdeII GATC 8 cut(s) 625, 707, 1009, 1192, 1489, 1684, 1798, 1992
NlaIII CATG 7 cut(s) 639, 1146, 1864, 1880, 2059, 2075, 2215
NlaIV GGNNCC 3 cut(s) 826, 914, 1883
NmuCI GTSAC 3 cut(s) 92, 803, 1932
NsiI ATGCAT 1 cut(s) 2162
NspI RCATGY 1 cut(s) 2075
NspV TTCGAA 1 cut(s) 902
OliI CACNNNNGTG 1 cut(s) 326
PaeR7I CTCGAG 1 cut(s) 151
PaqCI CACCTGC 2 cut(s) 70, 1060
PciI ACATGT 1 cut(s) 2071
PctI GAATGC 1 cut(s) 2160
PdmI GAANNNNTTC 3 cut(s) 1250, 1960, 2290
PfeI GAWTC 7 cut(s) 304, 904, 1588, 1753, 1772, 2117, 2192
PflMI CCANNNNNTGG 1 cut(s) 615
PfoI TCCNGGA 2 cut(s) 390, 1641
PinAI ACCGGT 1 cut(s) 798
PkrI GCNGC 7 cut(s) 787, 1030, 1127, 1291, 1380, 2082, 2268
PleI GAGTC 2 cut(s) 1664, 2137
PpsI GAGTC 2 cut(s) 1664, 2137
PscI ACATGT 1 cut(s) 2071
PshBI ATTAAT 2 cut(s) 855, 1131
PsiI TTATAA 1 cut(s) 1470
Psp124BI GAGCTC 1 cut(s) 158
Psp6I CCWGG 5 cut(s) 390, 529, 628, 1641, 1767
PspGI CCWGG 5 cut(s) 390, 529, 628, 1641, 1767
PspN4I GGNNCC 3 cut(s) 826, 914, 1883
PspPI GGNCC 7 cut(s) 318, 334, 632, 639, 1882, 2047, 2200
PspXI VCTCGAGB 1 cut(s) 151
PstI CTGCAG 1 cut(s) 1893
PsuI RGATCY 4 cut(s) 707, 1009, 1192, 1798
PvuII CAGCTG 1 cut(s) 2083
RsaI GTAC 4 cut(s) 269, 596, 777, 1305
RsaNI GTAC 4 cut(s) 268, 595, 776, 1304
RseI CAYNNNNRTG 3 cut(s) 68, 326, 2142
SacI GAGCTC 1 cut(s) 158
SatI GCNGC 7 cut(s) 786, 1029, 1126, 1290, 1379, 2081, 2267
Sau3AI GATC 8 cut(s) 625, 707, 1009, 1192, 1489, 1684, 1798, 1992
Sau96I GGNCC 7 cut(s) 318, 334, 632, 639, 1882, 2047, 2200
SchI GAGTC 2 cut(s) 1664, 2138
ScrFI CCNGG 7 cut(s) 144, 392, 531, 630, 882, 1643, 1769
SduI GDGCHC 2 cut(s) 158, 2175
SfaNI GCATC 4 cut(s) 745, 1816, 2147, 2255
SfcI CTRYAG 3 cut(s) 438, 544, 1889
Sfr274I CTCGAG 1 cut(s) 151
SfuI TTCGAA 1 cut(s) 902
SinI GGWCC 5 cut(s) 318, 334, 1882, 2047, 2200
SlaI CTCGAG 1 cut(s) 151
SmiMI CAYNNNNRTG 3 cut(s) 68, 326, 2142
SmlI CTYRAG 5 cut(s) 151, 550, 1078, 1334, 2152
SmoI CTYRAG 5 cut(s) 151, 550, 1078, 1334, 2152
SpeI ACTAGT 1 cut(s) 1049
SsiI CCGC 4 cut(s) 1126, 1289, 1292, 2298
SspI AATATT 4 cut(s) 587, 614, 853, 1872
SspMI CTAG 5 cut(s) 1050, 1184, 1362, 1823, 2126
SstI GAGCTC 1 cut(s) 158
StyD4I CCNGG 7 cut(s) 142, 390, 529, 628, 880, 1641, 1767
StyI CCWWGG 3 cut(s) 635, 1447, 1876
TaaI ACNGT 3 cut(s) 819, 968, 1465
TaqI TCGA 4 cut(s) 152, 902, 1022, 2371
TaqII GACCGA 3 cut(s) 322, 822, 2217
TatI WGTACW 1 cut(s) 594
TauI GCSGC 2 cut(s) 1128, 1292
TfiI GAWTC 7 cut(s) 304, 904, 1588, 1753, 1772, 2117, 2192
TscAI CASTG 7 cut(s) 75, 207, 355, 773, 824, 1468, 1953
TseFI GTSAC 3 cut(s) 92, 803, 1932
TseI GCWGC 5 cut(s) 785, 1028, 1378, 2080, 2266
Tsp45I GTSAC 3 cut(s) 92, 803, 1932
TspGWI ACGGA 4 cut(s) 285, 700, 734, 1193
TspRI CASTG 7 cut(s) 75, 207, 355, 773, 824, 1468, 1953
Van91I CCANNNNNTGG 1 cut(s) 615
Vha464I CTTAAG 2 cut(s) 1078, 1334
VpaK11BI GGWCC 5 cut(s) 318, 334, 1882, 2047, 2200
VspI ATTAAT 2 cut(s) 855, 1131
XapI RAATTY 2 cut(s) 376, 1544
XbaI TCTAGA 1 cut(s) 1822
XceI RCATGY 1 cut(s) 2075
XcmI CCANNNNNNNNNTGG 2 cut(s) 527, 1454
XhoI CTCGAG 1 cut(s) 151
XmnI GAANNNNTTC 3 cut(s) 1250, 1960, 2290
XspI CTAG 5 cut(s) 1050, 1184, 1362, 1823, 2126
Zsp2I ATGCAT 1 cut(s) 2162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.