divergent subfamily of APPLE domains
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 259 |
| AccII | CGCG | 1 cut(s) | 391 |
| AclI | AACGTT | 1 cut(s) | 364 |
| AcsI | RAATTY | 3 cut(s) | 65, 188, 513 |
| AfaI | GTAC | 2 cut(s) | 106, 293 |
| AgsI | TTSAA | 2 cut(s) | 193, 224 |
| AjnI | CCWGG | 3 cut(s) | 246, 419, 545 |
| AluBI | AGCT | 4 cut(s) | 53, 220, 244, 309 |
| AluI | AGCT | 4 cut(s) | 53, 220, 244, 309 |
| Alw26I | GTCTC | 1 cut(s) | 148 |
| Ama87I | CYCGRG | 1 cut(s) | 17 |
| AoxI | GGCC | 1 cut(s) | 178 |
| ApeKI | GCWGC | 1 cut(s) | 53 |
| ApoI | RAATTY | 3 cut(s) | 65, 188, 513 |
| AspLEI | GCGC | 1 cut(s) | 391 |
| AspS9I | GGNCC | 3 cut(s) | 13, 178, 313 |
| AvaI | CYCGRG | 1 cut(s) | 17 |
| AvaII | GGWCC | 2 cut(s) | 13, 313 |
| BbvI | GCAGC | 1 cut(s) | 40 |
| BciT130I | CCWGG | 3 cut(s) | 248, 421, 547 |
| BcoDI | GTCTC | 1 cut(s) | 148 |
| BfaI | CTAG | 1 cut(s) | 467 |
| BglII | AGATCT | 1 cut(s) | 442 |
| BisI | GCNGC | 1 cut(s) | 54 |
| BlsI | GCNGC | 1 cut(s) | 55 |
| Bme1390I | CCNGG | 3 cut(s) | 248, 421, 547 |
| Bme18I | GGWCC | 2 cut(s) | 13, 313 |
| BmeT110I | CYCGRG | 1 cut(s) | 17 |
| BmgT120I | GGNCC | 3 cut(s) | 13, 178, 313 |
| BmrFI | CCNGG | 3 cut(s) | 248, 421, 547 |
| BmsI | GCATC | 1 cut(s) | 242 |
| BsaJI | CCNNGG | 1 cut(s) | 247 |
| BsaWI | WCCGGW | 1 cut(s) | 228 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 53, 83, 165, 195, 349, 379 |
| BseBI | CCWGG | 3 cut(s) | 248, 421, 547 |
| BseDI | CCNNGG | 1 cut(s) | 247 |
| BseGI | GGATG | 1 cut(s) | 257 |
| BseMII | CTCAG | 1 cut(s) | 416 |
| BseXI | GCAGC | 1 cut(s) | 40 |
| Bsh1236I | CGCG | 1 cut(s) | 391 |
| BshFI | GGCC | 1 cut(s) | 180 |
| BsiHKCI | CYCGRG | 1 cut(s) | 17 |
| BsiSI | CCGG | 1 cut(s) | 229 |
| BsmAI | GTCTC | 1 cut(s) | 148 |
| BsmI | GAATGC | 1 cut(s) | 166 |
| BsnI | GGCC | 1 cut(s) | 180 |
| BsoBI | CYCGRG | 1 cut(s) | 17 |
| Bsp143I | GATC | 2 cut(s) | 442, 555 |
| BspANI | GGCC | 1 cut(s) | 180 |
| BspCNI | CTCAG | 1 cut(s) | 417 |
| BspFNI | CGCG | 1 cut(s) | 391 |
| BssECI | CCNNGG | 1 cut(s) | 247 |
| BssMI | GATC | 2 cut(s) | 442, 555 |
| BssNAI | GTATAC | 1 cut(s) | 260 |
| Bst1107I | GTATAC | 1 cut(s) | 260 |
| Bst2UI | CCWGG | 3 cut(s) | 248, 421, 547 |
| Bst4CI | ACNGT | 3 cut(s) | 109, 159, 473 |
| Bst6I | CTCTTC | 2 cut(s) | 3, 146 |
| BstC8I | GCNNGC | 1 cut(s) | 454 |
| BstDEI | CTNAG | 1 cut(s) | 425 |
| BstF5I | GGATG | 1 cut(s) | 257 |
| BstFNI | CGCG | 1 cut(s) | 391 |
| BstHHI | GCGC | 1 cut(s) | 391 |
| BstKTI | GATC | 2 cut(s) | 445, 558 |
| BstMAI | GTCTC | 1 cut(s) | 148 |
| BstMBI | GATC | 2 cut(s) | 442, 555 |
| BstNI | CCWGG | 3 cut(s) | 248, 421, 547 |
| BstSCI | CCNGG | 3 cut(s) | 246, 419, 545 |
| BstUI | CGCG | 1 cut(s) | 391 |
| BstV1I | GCAGC | 1 cut(s) | 40 |
| BstX2I | RGATCY | 1 cut(s) | 442 |
| BstYI | RGATCY | 1 cut(s) | 442 |
| BstZ17I | GTATAC | 1 cut(s) | 260 |
| BsuRI | GGCC | 1 cut(s) | 180 |
| BtsCI | GGATG | 1 cut(s) | 257 |
| BtsI | GCAGTG | 1 cut(s) | 153 |
| BtsIMutI | CAGTG | 2 cut(s) | 153, 478 |
| Cac8I | GCNNGC | 1 cut(s) | 454 |
| CfoI | GCGC | 1 cut(s) | 391 |
| Cfr13I | GGNCC | 3 cut(s) | 13, 178, 313 |
| Csp6I | GTAC | 2 cut(s) | 105, 292 |
| CviJI | RGCY | 7 cut(s) | 53, 99, 180, 220, 244, 309, 424 |
| CviKI_1 | RGCY | 7 cut(s) | 53, 99, 180, 220, 244, 309, 424 |
| CviQI | GTAC | 2 cut(s) | 105, 292 |
| DdeI | CTNAG | 1 cut(s) | 425 |
| DpnI | GATC | 2 cut(s) | 444, 557 |
| DpnII | GATC | 2 cut(s) | 442, 555 |
| Eam1104I | CTCTTC | 2 cut(s) | 3, 146 |
| EarI | CTCTTC | 2 cut(s) | 3, 146 |
| Eco47I | GGWCC | 2 cut(s) | 13, 313 |
| Eco88I | CYCGRG | 1 cut(s) | 17 |
| EcoO109I | RGGNCCY | 2 cut(s) | 13, 178 |
| EcoRI | GAATTC | 1 cut(s) | 188 |
| EcoRII | CCWGG | 3 cut(s) | 246, 419, 545 |
| FaiI | YATR | 7 cut(s) | 74, 112, 114, 260, 380, 438, 565 |
| FblI | GTMKAC | 1 cut(s) | 259 |
| Fnu4HI | GCNGC | 1 cut(s) | 54 |
| FokI | GGATG | 1 cut(s) | 264 |
| Fsp4HI | GCNGC | 1 cut(s) | 54 |
| FspBI | CTAG | 1 cut(s) | 467 |
| GlaI | GCGC | 1 cut(s) | 390 |
| GluI | GCNGC | 1 cut(s) | 54 |
| HaeIII | GGCC | 1 cut(s) | 180 |
| HapII | CCGG | 1 cut(s) | 229 |
| HhaI | GCGC | 1 cut(s) | 391 |
| Hin6I | GCGC | 1 cut(s) | 389 |
| HinP1I | GCGC | 1 cut(s) | 389 |
| HindIII | AAGCTT | 1 cut(s) | 218 |
| HinfI | GANTC | 3 cut(s) | 41, 232, 416 |
| HpaII | CCGG | 1 cut(s) | 229 |
| Hpy166II | GTNNAC | 1 cut(s) | 260 |
| Hpy188I | TCNGA | 3 cut(s) | 185, 496, 560 |
| Hpy188III | TCNNGA | 4 cut(s) | 171, 193, 208, 413 |
| Hpy8I | GTNNAC | 1 cut(s) | 260 |
| HpyAV | CCTTC | 2 cut(s) | 138, 305 |
| HpyCH4III | ACNGT | 3 cut(s) | 109, 159, 473 |
| HpyCH4IV | ACGT | 1 cut(s) | 364 |
| HpyCH4V | TGCA | 4 cut(s) | 4, 146, 166, 215 |
| HpyF3I | CTNAG | 1 cut(s) | 425 |
| HpySE526I | ACGT | 1 cut(s) | 364 |
| HspAI | GCGC | 1 cut(s) | 389 |
| Kzo9I | GATC | 2 cut(s) | 442, 555 |
| LmnI | GCTCC | 1 cut(s) | 249 |
| Lsp1109I | GCAGC | 1 cut(s) | 40 |
| LweI | GCATC | 1 cut(s) | 242 |
| MaeI | CTAG | 1 cut(s) | 467 |
| MaeII | ACGT | 1 cut(s) | 364 |
| MaeIII | GTNAC | 1 cut(s) | 21 |
| MalI | GATC | 2 cut(s) | 444, 557 |
| MboI | GATC | 2 cut(s) | 442, 555 |
| MboII | GAAGA | 4 cut(s) | 20, 163, 281, 398 |
| MflI | RGATCY | 1 cut(s) | 442 |
| MluCI | AATT | 8 cut(s) | 65, 84, 141, 188, 317, 384, 429, 513 |
| MnlI | CCTC | 7 cut(s) | 4, 26, 85, 191, 193, 351, 386 |
| MslI | CAYNNNNRTG | 1 cut(s) | 377 |
| MspA1I | CMGCKG | 1 cut(s) | 53 |
| MspI | CCGG | 1 cut(s) | 229 |
| MspR9I | CCNGG | 3 cut(s) | 248, 421, 547 |
| Mva1269I | GAATGC | 1 cut(s) | 166 |
| MvaI | CCWGG | 3 cut(s) | 248, 421, 547 |
| MvnI | CGCG | 1 cut(s) | 391 |
| NdeII | GATC | 2 cut(s) | 442, 555 |
| PaeR7I | CTCGAG | 1 cut(s) | 17 |
| PctI | GAATGC | 1 cut(s) | 166 |
| PfeI | GAWTC | 3 cut(s) | 41, 232, 416 |
| PkrI | GCNGC | 1 cut(s) | 55 |
| PpuMI | RGGWCCY | 1 cut(s) | 13 |
| Psp1406I | AACGTT | 1 cut(s) | 364 |
| Psp5II | RGGWCCY | 1 cut(s) | 13 |
| Psp6I | CCWGG | 3 cut(s) | 246, 419, 545 |
| PspGI | CCWGG | 3 cut(s) | 246, 419, 545 |
| PspPI | GGNCC | 3 cut(s) | 13, 178, 313 |
| PspPPI | RGGWCCY | 1 cut(s) | 13 |
| PspXI | VCTCGAGB | 1 cut(s) | 17 |
| PsuI | RGATCY | 1 cut(s) | 442 |
| PvuII | CAGCTG | 1 cut(s) | 53 |
| RsaI | GTAC | 2 cut(s) | 106, 293 |
| RsaNI | GTAC | 2 cut(s) | 105, 292 |
| RseI | CAYNNNNRTG | 1 cut(s) | 377 |
| SatI | GCNGC | 1 cut(s) | 54 |
| Sau3AI | GATC | 2 cut(s) | 442, 555 |
| Sau96I | GGNCC | 3 cut(s) | 13, 178, 313 |
| ScrFI | CCNGG | 3 cut(s) | 248, 421, 547 |
| SfaNI | GCATC | 1 cut(s) | 242 |
| Sfr274I | CTCGAG | 1 cut(s) | 17 |
| SinI | GGWCC | 2 cut(s) | 13, 313 |
| SlaI | CTCGAG | 1 cut(s) | 17 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 377 |
| SmlI | CTYRAG | 1 cut(s) | 17 |
| SmoI | CTYRAG | 1 cut(s) | 17 |
| Sse9I | AATT | 8 cut(s) | 65, 84, 141, 188, 317, 384, 429, 513 |
| SspMI | CTAG | 1 cut(s) | 467 |
| StyD4I | CCNGG | 3 cut(s) | 246, 419, 545 |
| TaaI | ACNGT | 3 cut(s) | 109, 159, 473 |
| TaiI | ACGT | 1 cut(s) | 367 |
| TaqI | TCGA | 1 cut(s) | 18 |
| TasI | AATT | 8 cut(s) | 65, 84, 141, 188, 317, 384, 429, 513 |
| TfiI | GAWTC | 3 cut(s) | 41, 232, 416 |
| TscAI | CASTG | 2 cut(s) | 153, 478 |
| TseI | GCWGC | 1 cut(s) | 53 |
| TspDTI | ATGAA | 2 cut(s) | 398, 494 |
| TspRI | CASTG | 2 cut(s) | 153, 478 |
| VpaK11BI | GGWCC | 2 cut(s) | 13, 313 |
| XapI | RAATTY | 3 cut(s) | 65, 188, 513 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 307 |
| XhoI | CTCGAG | 1 cut(s) | 17 |
| XmiI | GTMKAC | 1 cut(s) | 259 |
| XspI | CTAG | 1 cut(s) | 467 |