FvH4_3g03350

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000112
Physical Location & Seq
Forward (+)
1818089 .. 1821332
3244 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g03350.t1

Sequence Viewer

Length: 2493 bp
ATGAAAAGATCAACGTGTTATATTTCCGATCAGCCAACAGAGAAAATGAGAAAAAATGCCCTGTTTCGCACCTACATAGTTGCAATGTTTCGCAGAGCTCTGTTTCTCTTCCTCCTCTTTCAGTTCTGCACTTCCAGGGACACCATAAGAGGGAACGAAGAGCAACTGAAGGATGTTGATGGAGACTATCTACTTTCCAGAGAAAGCAAGTTTGAGTTGGGGTTCTTCAGCCCTGGAAATTCTAGCTACCGGTATGTTGGAATCTGGTATTCTCAGACTAGAGCAACTGAAAAAACAGTGGTGTGGGTTGCAAACAGGAACAATCCCATCAATGATACGTCTGGTGTGCTCACAATCAACAGGTATGGAGAACTGGTCCTCTATGCTTACAATTTGGAAAATGATCCTATATGGTTCACCAATACGTCGCAGTCGGTCAAGAATGTTAACACTAGCACTTTATCAGCACAGCTTTTGGATACAGGAAATTTTGTTGTGTTCCAGGATGATAATAATGATATATTTATATGGCAGAGTTTTGATCATCCTACAGATACTCTACTTCCAGGTATGAAACTTGGGGTGAATTGGAAAACTGGGCTAGAATGGGTTTTAACATCTTGGAAGTCACAAGATGACCCTGGAACTGGGGACTCTACCTTTAGGCTCTATCCAAGCCAGATTGGATTCCCCCAATTTTTTATGTATAAGGGTTTGAGTAAGTATTGGCGAGCTGATCCTGGGCCAACGCCTAATGTGGTCAATAATCAAGAGGAAACTTATAATTTCTTGAATGCCGATGCAATTACAAGAACAATAGTTACTGATTCTGCGGTGAAGCGCCTTACTTGGGATGATGGCAACCTTCAATGGAAGGAAGACTTATCTGCACCAAAGTCCCGGTGTGACTGGTATGGACGGTGTGGTGCCAACAGCAAATGTAGCCCTGACAATGTTAATCAGTTTGAGTGTGAATGCTTACCAGGGTATGAGCCTAAGTCTATTAGTGATTGGAATCAGAAAAATGGGTCAGGTGGATGTGTTAATAATAGGGTTGGTTTGTTCAAGTGTGGAGATGGAGATGGGTTTGTGAAGGTGGAAAGAGTTAAATATCCGGACACATTAATAGCAGCATTGTCAAAATCAGGCATGAGTGCCAAAGAGTGTCGGCATGAGTGCCTAAGAAATTGTTCTTGCACTGCATATTTGAGCACTGAAAATGAAGGGCTTGATGGCTGCTTCACATGGTATGATGACCTGATGGACATTTTAGGGTACACAGAGGTTGGACGAGATCTCTACGTTCGTGTGAATGCAACTGTGTTAGCTGCATATGTAGGAAAATCACAAGGTTTCTTGGAAAGAAAGGGTATGCTGGCTATTCCAATACTGTCTGCTGTGCTAGCGTTGGTACTAACCATCATGCTCGGCTGTTGGTGGCGAAAGAAGAACCACAACACAAAAGACATTCTGCAGGGAGAGGCGCTTGATGAAACACAGAGACACCCCGAATTACAATTTTTTGATCTTGACACAATAATAGCAGCCACAGACCACTTTTCTCGTATCAATGAACTTGGCCATGGTGGTTTTGGCTCTGTTTATAAGGGTAAGCTACCAAATGAACAAAATGTTGCTGTCAAAAGGTTGTCCAAAACTTCAGGACAAGGGATTGAAGAATTCAAAAATGAAGTTGCACTTATAGCAAGACTTCAACACAGGAACCTCGTGAAACTTTTAGGCTGCTGTATAATGGGAGAAGAGAGAATTTTAGTCCTAGAATACATGCCTAACAAAAGCTTGGATTCCTTTCTTTTTGATCACACAAGACGTTGGGTCTTGGACTGGGAAAGGCGCTTTGAGATCATCAATGGGATTGCTCGTGGAATTCTGTATCTTCACCAAGATTCAAGATTGAGAATCATCCATAGAGATCTGAAACCTAGCAATGTTCTACTAGATGCAGAGATGAACCCAAAAATTTCTGATTTTGGCATGGCTAGAATATTCCATGGGAACCAACTTCAAGACAAAACGAGCCGAATTGTTGGAACATATGGCTACATGTCACCAGAATACGCAGTATTTGGGAGATTTTCCATGAAATCTGACGTCTTTAGTTTTGGGATCATAATGTTGGAGATTGTAAGTGGCCAGAAAATCAATGGTTCTGGTCTAGAGGATCCTTCCATGAATTTGATAGGACATGTTTGGGAGCTCTGGAATGAAGGTAGAGCCTTAGATATTGTGGATTCGACATTGAAGTCATATCAGTCTGATGAAGTCATTAGATGCATACAAGTTGCTCTCTTGTGTGTACAAGAAGATTCAAAGGACCGACCTCCCATGTCAGCTGTTGTTTTCATGTTGAGTGGTGAAGCATCTCGTCCATCTCCTAAGCAGCCGGCATTTGTCTACAGAAAATACTCCGATGCTGATGATGATCCATTACTTTCAAACGACTTGACAGTAACTATAATGGAAGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

831

Amino Acids

94.3

Weight (kDa)

5.81

Isoelectric Point (pI)

35.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 99 - 209 5.7e-36 D-mannose binding lectin
S_locus_glycop PF00954 255 - 334 5.3e-14 S-locus glycoprotein domain
PAN_2 PF08276 357 - 423 3.3e-18 PAN-like domain
Pkinase PF00069 521 - 782 1.3e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 523 - 787 4.1e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 783, 1605
AasI GACNNNNNNGTC 1 cut(s) 2263
AatII GACGTC 1 cut(s) 2115
AccB1I GGYRCC 1 cut(s) 926
AccI GTMKAC 1 cut(s) 2415
AccIII TCCGGA 1 cut(s) 1114
AciI CCGC 1 cut(s) 833
AclWI GGATC 6 cut(s) 398, 731, 2135, 2177, 2190, 2438
AcoI YGGCCR 2 cut(s) 1579, 2152
AcsI RAATTY 7 cut(s) 238, 487, 1679, 1767, 1887, 1980, 2194
AcuI CTGAAG 3 cut(s) 188, 211, 1644
AcyI GRCGYC 1 cut(s) 2112
AfaI GTAC 3 cut(s) 1277, 1413, 2319
AfiI CCNNNNNNNGG 3 cut(s) 150, 647, 850
AflIII ACRYGT 3 cut(s) 14, 2064, 2206
AgeI ACCGGT 1 cut(s) 249
AhdI GACNNNNNGTC 1 cut(s) 1835
AjnI CCWGG 7 cut(s) 134, 232, 501, 565, 640, 739, 982
Alw21I GWGCWC 4 cut(s) 100, 351, 1214, 2220
Alw26I GTCTC 2 cut(s) 177, 1495
AlwI GGATC 6 cut(s) 398, 731, 2135, 2177, 2190, 2438
Aor13HI TCCGGA 1 cut(s) 1114
AoxI GGCC 3 cut(s) 743, 1579, 2152
ApeKI GCWGC 6 cut(s) 1130, 1236, 1328, 1544, 1743, 2401
ApoI RAATTY 7 cut(s) 238, 487, 1679, 1767, 1887, 1980, 2194
AseI ATTAAT 1 cut(s) 1124
AsiGI ACCGGT 1 cut(s) 249
Asp700I GAANNNNTTC 1 cut(s) 1189
AspLEI GCGC 3 cut(s) 843, 1486, 1857
AspS9I GGNCC 3 cut(s) 376, 743, 2335
AsuC2I CCSGG 1 cut(s) 901
AsuHPI GGTGA 6 cut(s) 409, 595, 847, 1892, 2061, 2387
AsuNHI GCTAGC 1 cut(s) 1402
AvaII GGWCC 2 cut(s) 376, 2335
BalI TGGCCA 2 cut(s) 1581, 2154
BamHI GGATCC 1 cut(s) 2182
BanI GGYRCC 1 cut(s) 926
BanII GRGCYC 2 cut(s) 100, 2220
BauI CACGAG 2 cut(s) 1727, 1881
BbsI GAAGAC 1 cut(s) 885
Bbv12I GWGCWC 4 cut(s) 100, 351, 1214, 2220
BbvI GCAGC 6 cut(s) 1142, 1223, 1315, 1556, 1730, 2413
BccI CCATC 9 cut(s) 173, 335, 851, 1070, 1076, 1226, 1255, 1427, 2398
BciT130I CCWGG 7 cut(s) 136, 234, 503, 567, 642, 741, 984
BciVI GTATCC 1 cut(s) 472
BclI TGATCA 2 cut(s) 541, 1819
BcnI CCSGG 1 cut(s) 901
BcoDI GTCTC 2 cut(s) 177, 1495
BfmI CTRYAG 3 cut(s) 549, 1472, 2416
BfoI RGCGCY 3 cut(s) 844, 1487, 1858
BfuI GTATCC 1 cut(s) 472
BglII AGATCT 2 cut(s) 1294, 1933
BisI GCNGC 6 cut(s) 1131, 1237, 1329, 1545, 1744, 2402
BlsI GCNGC 6 cut(s) 1132, 1238, 1330, 1546, 1745, 2403
Bme1390I CCNGG 8 cut(s) 136, 234, 503, 567, 642, 741, 901, 984
Bme18I GGWCC 2 cut(s) 376, 2335
BmeRI GACNNNNNGTC 1 cut(s) 1835
BmgT120I GGNCC 3 cut(s) 376, 743, 2335
BmiI GGNNCC 4 cut(s) 928, 1724, 2018, 2184
BmrFI CCNGG 8 cut(s) 136, 234, 503, 567, 642, 741, 901, 984
BmrI ACTGGG 3 cut(s) 606, 657, 1855
BmsI GCATC 5 cut(s) 790, 1951, 2282, 2390, 2422
BmtI GCTAGC 1 cut(s) 1406
BmuI ACTGGG 3 cut(s) 606, 657, 1855
BpiI GAAGAC 1 cut(s) 885
Bpu10I CCTNAGC 1 cut(s) 2397
BpuMI CCSGG 1 cut(s) 901
BsaBI GATNNNNATC 2 cut(s) 1014, 2442
BsaHI GRCGYC 1 cut(s) 2112
BsaJI CCNNGG 7 cut(s) 135, 232, 640, 740, 983, 1582, 2011
BsaWI WCCGGW 2 cut(s) 249, 1114
BsaXI ACNNNNNCTCC 2 cut(s) 360, 390
Bsc4I CCNNNNNNNGG 3 cut(s) 150, 647, 850
Bse118I RCCGGY 2 cut(s) 249, 2404
Bse1I ACTGG 5 cut(s) 378, 601, 652, 914, 1850
Bse3DI GCAATG 2 cut(s) 90, 1954
Bse8I GATNNNNATC 2 cut(s) 1014, 2442
BseAI TCCGGA 1 cut(s) 1114
BseBI CCWGG 7 cut(s) 136, 234, 503, 567, 642, 741, 984
BseDI CCNNGG 7 cut(s) 135, 232, 640, 740, 983, 1582, 2011
BseGI GGATG 6 cut(s) 178, 511, 544, 859, 1043, 1923
BseJI GATNNNNATC 2 cut(s) 1014, 2442
BseLI CCNNNNNNNGG 3 cut(s) 150, 647, 850
BseMI GCAATG 2 cut(s) 90, 1954
BseMII CTCAG 1 cut(s) 287
BseNI ACTGG 5 cut(s) 378, 601, 652, 914, 1850
BseRI GAGGAG 1 cut(s) 104
BseXI GCAGC 6 cut(s) 1142, 1223, 1315, 1556, 1730, 2413
BsgI GTGCAG 2 cut(s) 112, 873
BshFI GGCC 3 cut(s) 745, 1581, 2154
BshNI GGYRCC 1 cut(s) 926
BshTI ACCGGT 1 cut(s) 249
BsiHKAI GWGCWC 4 cut(s) 100, 351, 1214, 2220
BsiSI CCGG 4 cut(s) 250, 901, 1115, 2405
BslFI GGGAC 3 cut(s) 152, 665, 883
BslI CCNNNNNNNGG 3 cut(s) 150, 647, 850
BsmAI GTCTC 2 cut(s) 177, 1495
BsmFI GGGAC 3 cut(s) 152, 665, 883
BsmI GAATGC 3 cut(s) 799, 980, 1318
BsnI GGCC 3 cut(s) 745, 1581, 2154
Bsp1286I GDGCHC 4 cut(s) 100, 351, 1214, 2220
Bsp13I TCCGGA 1 cut(s) 1114
Bsp1407I TGTACA 1 cut(s) 2317
Bsp19I CCATGG 2 cut(s) 1582, 2011
BspACI CCGC 1 cut(s) 833
BspANI GGCC 3 cut(s) 745, 1581, 2154
BspCNI CTCAG 1 cut(s) 286
BspEI TCCGGA 1 cut(s) 1114
BspLI GGNNCC 4 cut(s) 928, 1724, 2018, 2184
BspMAI CTGCAG 1 cut(s) 1476
BspOI GCTAGC 1 cut(s) 1406
BspPI GGATC 6 cut(s) 398, 731, 2135, 2177, 2190, 2438
BspQI GCTCTTC 1 cut(s) 153
BspT107I GGYRCC 1 cut(s) 926
BsrDI GCAATG 2 cut(s) 90, 1954
BsrFI RCCGGY 2 cut(s) 249, 2404
BsrGI TGTACA 1 cut(s) 2317
BsrI ACTGG 5 cut(s) 378, 601, 652, 914, 1850
BssAI RCCGGY 2 cut(s) 249, 2404
BssECI CCNNGG 7 cut(s) 135, 232, 640, 740, 983, 1582, 2011
BssNI GRCGYC 1 cut(s) 2112
BssSI CACGAG 2 cut(s) 1727, 1881
BssT1I CCWWGG 2 cut(s) 1582, 2011
Bst2BI CACGAG 2 cut(s) 1727, 1881
Bst2UI CCWGG 7 cut(s) 136, 234, 503, 567, 642, 741, 984
Bst4CI ACNGT 5 cut(s) 298, 921, 1321, 1392, 2470
Bst6I CTCTTC 3 cut(s) 113, 153, 1755
BstACI GRCGYC 1 cut(s) 2112
BstAUI TGTACA 1 cut(s) 2317
BstC8I GCNNGC 4 cut(s) 732, 1377, 1404, 2406
BstDEI CTNAG 5 cut(s) 273, 996, 1181, 2239, 2397
BstDSI CCRYGG 2 cut(s) 1582, 2011
BstF5I GGATG 6 cut(s) 178, 511, 544, 859, 1043, 1923
BstH2I RGCGCY 3 cut(s) 844, 1487, 1858
BstHHI GCGC 3 cut(s) 843, 1486, 1857
BstMAI GTCTC 2 cut(s) 177, 1495
BstMWI GCNNNNNNNGC 3 cut(s) 942, 1403, 1703
BstNI CCWGG 7 cut(s) 136, 234, 503, 567, 642, 741, 984
BstNSI RCATGY 3 cut(s) 1789, 2068, 2210
BstSCI CCNGG 8 cut(s) 134, 232, 501, 565, 640, 739, 899, 982
BstSFI CTRYAG 3 cut(s) 549, 1472, 2416
BstV1I GCAGC 6 cut(s) 1142, 1223, 1315, 1556, 1730, 2413
BstV2I GAAGAC 1 cut(s) 885
BstX2I RGATCY 3 cut(s) 1294, 1933, 2182
BstYI RGATCY 3 cut(s) 1294, 1933, 2182
BsuI GTATCC 1 cut(s) 472
BsuRI GGCC 3 cut(s) 745, 1581, 2154
BtgI CCRYGG 2 cut(s) 1582, 2011
BtsCI GGATG 6 cut(s) 178, 511, 544, 859, 1043, 1923
BtsI GCAGTG 1 cut(s) 1197
BtsIMutI CAGTG 3 cut(s) 303, 1197, 1212
Cac8I GCNNGC 4 cut(s) 732, 1377, 1404, 2406
CfoI GCGC 3 cut(s) 843, 1486, 1857
Cfr10I RCCGGY 2 cut(s) 249, 2404
Cfr13I GGNCC 3 cut(s) 376, 743, 2335
Csp6I GTAC 3 cut(s) 1276, 1412, 2318
CspAI ACCGGT 1 cut(s) 249
CviQI GTAC 3 cut(s) 1276, 1412, 2318
DdeI CTNAG 5 cut(s) 273, 996, 1181, 2239, 2397
DrdI GACNNNNNNGTC 1 cut(s) 2263
DriI GACNNNNNGTC 1 cut(s) 1835
DseDI GACNNNNNNGTC 1 cut(s) 2263
EaeI YGGCCR 2 cut(s) 1579, 2152
Eam1104I CTCTTC 3 cut(s) 113, 153, 1755
Eam1105I GACNNNNNGTC 1 cut(s) 1835
EarI CTCTTC 3 cut(s) 113, 153, 1755
Ecl136II GAGCTC 2 cut(s) 98, 2218
Eco130I CCWWGG 2 cut(s) 1582, 2011
Eco24I GRGCYC 2 cut(s) 100, 2220
Eco47I GGWCC 2 cut(s) 376, 2335
Eco53kI GAGCTC 2 cut(s) 98, 2218
Eco57I CTGAAG 3 cut(s) 188, 211, 1644
EcoICRI GAGCTC 2 cut(s) 98, 2218
EcoRI GAATTC 2 cut(s) 1679, 1887
EcoRII CCWGG 7 cut(s) 134, 232, 501, 565, 640, 739, 982
EcoT14I CCWWGG 2 cut(s) 1582, 2011
EcoT22I ATGCAT 1 cut(s) 2297
EcoT38I GRGCYC 2 cut(s) 100, 2220
ErhI CCWWGG 2 cut(s) 1582, 2011
FalI AAGNNNNNCTT 4 cut(s) 866, 898, 1683, 1715
FaqI GGGAC 3 cut(s) 152, 665, 883
FauNDI CATATG 2 cut(s) 1333, 2056
FbaI TGATCA 2 cut(s) 541, 1819
FblI GTMKAC 1 cut(s) 2415
Fnu4HI GCNGC 6 cut(s) 1131, 1237, 1329, 1545, 1744, 2402
FokI GGATG 6 cut(s) 185, 518, 531, 866, 1050, 1910
FriOI GRGCYC 2 cut(s) 100, 2220
Fsp4HI GCNGC 6 cut(s) 1131, 1237, 1329, 1545, 1744, 2402
GlaI GCGC 3 cut(s) 842, 1485, 1856
GluI GCNGC 6 cut(s) 1131, 1237, 1329, 1545, 1744, 2402
HaeII RGCGCY 3 cut(s) 844, 1487, 1858
HaeIII GGCC 3 cut(s) 745, 1581, 2154
HapII CCGG 4 cut(s) 250, 901, 1115, 2405
HhaI GCGC 3 cut(s) 843, 1486, 1857
Hin1I GRCGYC 1 cut(s) 2112
Hin6I GCGC 3 cut(s) 841, 1484, 1855
HinP1I GCGC 3 cut(s) 841, 1484, 1855
HincII GTYRAC 1 cut(s) 448
HindII GTYRAC 1 cut(s) 448
HindIII AAGCTT 1 cut(s) 1798
HpaI GTTAAC 1 cut(s) 448
HpaII CCGG 4 cut(s) 250, 901, 1115, 2405
HphI GGTGA 6 cut(s) 409, 595, 847, 1892, 2061, 2387
Hpy166II GTNNAC 5 cut(s) 417, 448, 1278, 2318, 2416
Hpy188I TCNGA 8 cut(s) 28, 276, 1020, 1938, 1987, 2110, 2278, 2431
Hpy8I GTNNAC 5 cut(s) 417, 448, 1278, 2318, 2416
Hpy99I CGWCG 1 cut(s) 430
HpyAV CCTTC 7 cut(s) 163, 868, 875, 1087, 1217, 2196, 2222
HpyCH4III ACNGT 5 cut(s) 298, 921, 1321, 1392, 2470
HpyCH4IV ACGT 6 cut(s) 14, 338, 425, 1302, 1831, 2112
HpyF10VI GCNNNNNNNGC 3 cut(s) 942, 1403, 1703
HpyF3I CTNAG 5 cut(s) 273, 996, 1181, 2239, 2397
HpySE526I ACGT 6 cut(s) 14, 338, 425, 1302, 1831, 2112
Hsp92I GRCGYC 1 cut(s) 2112
HspAI GCGC 3 cut(s) 841, 1484, 1855
Kpn2I TCCGGA 1 cut(s) 1114
KroI GCCGGC 1 cut(s) 2404
KroNI GCCGGC 1 cut(s) 2406
Ksp22I TGATCA 2 cut(s) 541, 1819
KspAI GTTAAC 1 cut(s) 448
LguI GCTCTTC 1 cut(s) 153
LmnI GCTCC 1 cut(s) 2215
Lsp1109I GCAGC 6 cut(s) 1142, 1223, 1315, 1556, 1730, 2413
LweI GCATC 5 cut(s) 790, 1951, 2282, 2390, 2422
MaeII ACGT 6 cut(s) 14, 338, 425, 1302, 1831, 2112
MaeIII GTNAC 5 cut(s) 627, 820, 905, 2067, 2470
MboII GAAGA 9 cut(s) 100, 170, 217, 890, 1459, 1688, 1772, 1889, 2336
MflI RGATCY 3 cut(s) 1294, 1933, 2182
MhlI GDGCHC 4 cut(s) 100, 351, 1214, 2220
MlsI TGGCCA 2 cut(s) 1581, 2154
MluNI TGGCCA 2 cut(s) 1581, 2154
MlyI GAGTC 1 cut(s) 647
MmeI TCCRAC 4 cut(s) 238, 1267, 2029, 2118
Mox20I TGGCCA 2 cut(s) 1581, 2154
Mph1103I ATGCAT 1 cut(s) 2297
MroI TCCGGA 1 cut(s) 1114
MroNI GCCGGC 1 cut(s) 2404
MroXI GAANNNNTTC 1 cut(s) 1189
MscI TGGCCA 2 cut(s) 1581, 2154
MseI TTAA 6 cut(s) 447, 614, 957, 1044, 1107, 1124
Msp20I TGGCCA 2 cut(s) 1581, 2154
MspA1I CMGCKG 1 cut(s) 2354
MspI CCGG 4 cut(s) 250, 901, 1115, 2405
MspR9I CCNGG 8 cut(s) 136, 234, 503, 567, 642, 741, 901, 984
Mva1269I GAATGC 3 cut(s) 799, 980, 1318
MvaI CCWGG 7 cut(s) 136, 234, 503, 567, 642, 741, 984
MwoI GCNNNNNNNGC 3 cut(s) 942, 1403, 1703
NaeI GCCGGC 1 cut(s) 2406
NciI CCSGG 1 cut(s) 901
NcoI CCATGG 2 cut(s) 1582, 2011
NdeI CATATG 2 cut(s) 1333, 2056
NgoMIV GCCGGC 1 cut(s) 2404
NheI GCTAGC 1 cut(s) 1402
NlaIV GGNNCC 4 cut(s) 928, 1724, 2018, 2184
NmeAIII GCCGAG 1 cut(s) 1407
NmuCI GTSAC 3 cut(s) 627, 905, 2067
NsiI ATGCAT 1 cut(s) 2297
NspI RCATGY 3 cut(s) 1789, 2068, 2210
PciI ACATGT 2 cut(s) 2064, 2206
PciSI GCTCTTC 1 cut(s) 153
PctI GAATGC 3 cut(s) 799, 980, 1318
PdiI GCCGGC 1 cut(s) 2406
PdmI GAANNNNTTC 1 cut(s) 1189
PfeI GAWTC 9 cut(s) 261, 687, 827, 1015, 1805, 1907, 1920, 2252, 2327
PfoI TCCNGGA 1 cut(s) 501
PinAI ACCGGT 1 cut(s) 249
PkrI GCNGC 6 cut(s) 1132, 1238, 1330, 1546, 1745, 2403
PleI GAGTC 1 cut(s) 647
PpsI GAGTC 1 cut(s) 647
PscI ACATGT 2 cut(s) 2064, 2206
PshBI ATTAAT 1 cut(s) 1124
PsiI TTATAA 2 cut(s) 783, 1605
Psp124BI GAGCTC 2 cut(s) 100, 2220
Psp6I CCWGG 7 cut(s) 134, 232, 501, 565, 640, 739, 982
PspGI CCWGG 7 cut(s) 134, 232, 501, 565, 640, 739, 982
PspN4I GGNNCC 4 cut(s) 928, 1724, 2018, 2184
PspPI GGNCC 3 cut(s) 376, 743, 2335
PsrI GAACNNNNNNTAC 2 cut(s) 805, 837
PstI CTGCAG 1 cut(s) 1476
PsuI RGATCY 3 cut(s) 1294, 1933, 2182
PvuII CAGCTG 1 cut(s) 2354
RsaI GTAC 3 cut(s) 1277, 1413, 2319
RsaNI GTAC 3 cut(s) 1276, 1412, 2318
SacI GAGCTC 2 cut(s) 100, 2220
SapI GCTCTTC 1 cut(s) 153
SaqAI TTAA 6 cut(s) 447, 614, 957, 1044, 1107, 1124
SatI GCNGC 6 cut(s) 1131, 1237, 1329, 1545, 1744, 2402
Sau96I GGNCC 3 cut(s) 376, 743, 2335
SchI GAGTC 1 cut(s) 647
ScrFI CCNGG 8 cut(s) 136, 234, 503, 567, 642, 741, 901, 984
SduI GDGCHC 4 cut(s) 100, 351, 1214, 2220
SfaNI GCATC 5 cut(s) 790, 1951, 2282, 2390, 2422
SfcI CTRYAG 3 cut(s) 549, 1472, 2416
SinI GGWCC 2 cut(s) 376, 2335
SsiI CCGC 1 cut(s) 833
SspI AATATT 1 cut(s) 2007
SstI GAGCTC 2 cut(s) 100, 2220
StyD4I CCNGG 8 cut(s) 134, 232, 501, 565, 640, 739, 899, 982
StyI CCWWGG 2 cut(s) 1582, 2011
TaaI ACNGT 5 cut(s) 298, 921, 1321, 1392, 2470
TaiI ACGT 6 cut(s) 17, 341, 428, 1305, 1834, 2115
TaqI TCGA 2 cut(s) 2255, 2488
TaqII GACCGA 2 cut(s) 424, 2352
TatI WGTACW 1 cut(s) 2317
TfiI GAWTC 9 cut(s) 261, 687, 827, 1015, 1805, 1907, 1920, 2252, 2327
Tru1I TTAA 6 cut(s) 447, 614, 957, 1044, 1107, 1124
Tru9I TTAA 6 cut(s) 447, 614, 957, 1044, 1107, 1124
TscAI CASTG 3 cut(s) 303, 1204, 1219
TseFI GTSAC 3 cut(s) 627, 905, 2067
TseI GCWGC 6 cut(s) 1130, 1236, 1328, 1544, 1743, 2401
Tsp45I GTSAC 3 cut(s) 627, 905, 2067
TspRI CASTG 3 cut(s) 303, 1204, 1219
VpaK11BI GGWCC 2 cut(s) 376, 2335
VspI ATTAAT 1 cut(s) 1124
XapI RAATTY 7 cut(s) 238, 487, 1679, 1767, 1887, 1980, 2194
XbaI TCTAGA 1 cut(s) 2176
XceI RCATGY 3 cut(s) 1789, 2068, 2210
XcmI CCANNNNNNNNNTGG 2 cut(s) 1589, 2162
XmiI GTMKAC 1 cut(s) 2415
XmnI GAANNNNTTC 1 cut(s) 1189
ZraI GACGTC 1 cut(s) 2113
Zsp2I ATGCAT 1 cut(s) 2297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.