Rroxscaffold_6G00423590

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000112
Physical Location & Seq
Reverse (-)
44667653 .. 44670770
3118 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00423590.1

Sequence Viewer

Length: 2460 bp
ATGCCACTGTTTCACACCTACAAAGTTGCTAATGCGCAGAAAATGTTATTCAAAGCTCTGCTTCTCTTCCTCCTCTTCCGATTTTGCGAATCCAGGGACACCATAACATGGAACCAAGAACAACTGAAGGATGATGGTGGAGTTTTAGTGTCCAAACAGAGCAAGTTCGAATTGGGTTTCTTCAGCCCCGGAAATTCTAGCTACCGGTATGTCGGAATTTGGTATCCTCAGACTGAAGTATCTAATAAAACAGTGGTGTGGGTTGCAAACAGGAACAATCCCATCAATGATACCTCTGGTGTGCTCACAATAAACAGGTATGGAGAACTAGTCCTTTATGCTTATAAAATGGAGAGCACTCCTATTTGGTCTACTAATGTGTCGCGGTCGATTCAAAATGTTAACACAACCACTTTATCTGCACAGCTTTTAGATACAGGAAATCTAGTTGTGTTCCAGGATGATAATAATGAAATCTCTATATGGCAAAGTTTTGATCATCCTACAGATACTTTAATTCGAGGTATGAAAGTTGGGGTGAACTGGAAAACTGGGCTAGAATGGGTTTTAACATCTTGGAAGTCACAAGATGACCCTGGAACTGGGGACTATACCCTTAGGCTCTATCCAAATCAGATTGCATCCCCCCAATTTTTTATGTACAAGGGGTCGAGTAAGTATTGGCGAAGTGATACAGGGCCAATTGATTTGTTCACTAATCAAGAGGAGACTTATTATCTCATGAATTACACCAATACAATTACAAGAGTAACAGTGACTGATTCTGCGGTAGAACGCCTTACATGGGATGATGGTGGTCTTCAATGGGAGGAAGACTTCTCTGCACCGAAGTCCCGGTGTGACTGGTATGGACAGTGCGGTGCCAACAGCAAATGTAGCCCTGACAATGTTAATATGTTTTGGTGTGATTGTTTACCAGGGTATGTGCCTAATTCTATTAGTGATTGGAATCATAAAAATGGGACACGTGGATGTGTGAGTAATCGACTTGGTTTGTTGAAGTGTGGAGATGGAGACGGGTTTGTGAAGGTGGCAAGAGTTAAATATCCTGACACATCGATGGGAGCATTGTTAAAATCAGGTATGAGTGCCAAAGAGTGTGAGCAGGAGTGCCTAAGAAATTGTTCTTGCACCGCATATTTGAGCACAGAATATGAAGGGCTTGTTGATTGCTTGACATGGTTTGATGACTTGATGGACATTTTAGTGTACACAGAGCTTGGACAAGATCTCTACGTTCGTGTGAATGCAACCGTGTTAGCTGCAAATGCCAGAAGATCAGAAGGTTTCTTGGAAAGGAGGGGAATGCTGGCTATTCCAATACCGTCTGCTGTGCTAGCATTGGTACTAATCATTATGCTTGCACGTTGGTGGGGTAAGAAGAACAGGAACACAAAAGGCATAGACTTTGTGGAGGCAGACGAGCTTCTGGAAAACCAGAGAAACCCTGAATTGCAATTTTTCGATCTTGACACAATAATAGCAGCCACAGACCACTTCTCTCGTGTCAATGAACTTGGCCATGGTGGTTTTGGCTCTGTTTATAAGGGTCAGCTACCAAATGAAGAGAAAGTTGCTGTGAAAAGATTGTCCCAAACTTCAGGACAAGGGATCGAAGAATTCAAAAATGAAGTTGCAGTTATAGCAAGACTTCAACACAGGAATCTTGTGAAACTTTTAGGCTGTTGTATAAAGGGAGAAGAAAGGATGTTAGTGCTAGAATACATGCCTAACAAAAGCTTGAACTCCTTTCTTTTCGATAACACAAGACGGTCCATCTTGGATTGGGAAAAGCGCTTTGAAATAATCAACGGGATTGCTCGTGGGATTTTGTATCTTCACCAAGACTCAAGATTGAGGATCATCCATAGAGATCTGAAAACTAGCAATATTCTTCTAGATACGAAGATGAACCCAAAAATTTCTGATTTTGGCATGGCTAGAATATTCCACGGAGATCAACTGCAAGATAAGACGAGCCTGGCTACATGTCACCGAGTACGCAATATTTGGAAGATTTTCTACGAAATCGATGTATTTAGTTTTGGGATCATAATGTTGGAGATTGTCGGTGGTCGGAAAAACAATGGTTATGATCCGGAGGATCCTTCCATGAACTTGATAGGACGTGTAAGCAAAACATATTTTTCTAACATTTTATCCCTTAGTATTCATTTGCTTTTTAAACTAAATTTCAACTACAGGTTTGGGAGCTCGGGGAGAAGGCGAGTGGCCTTAGATATTGTTGATTCGACACCGAAGTCATATCAGCCTAATGAAGTCATGAGATGCATACAAGTTGCGCTCTTGTGTGTACAAGAAGATTCAAAGGACCGACCTGCCATGTCGCCCATTGTTTTTATGTTGAGTGGTGAAGCATCTCCTCCATTGCCTAAGCATCCGCATTTGTTTACGAAGAGATTTAAGCGCCGATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

819

Amino Acids

93.49

Weight (kDa)

7.96

Isoelectric Point (pI)

40.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 84 - 194 1.9e-34 D-mannose binding lectin
S_locus_glycop PF00954 240 - 318 1e-12 S-locus glycoprotein domain
PAN_2 PF08276 342 - 408 5.4e-18 PAN-like domain
Pkinase PF00069 508 - 703 3.2e-36 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 510 - 743 1.3e-39 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 345, 1566
AasI GACNNNNNNGTC 1 cut(s) 2281
Acc16I TGCGCA 1 cut(s) 36
Acc36I ACCTGC 1 cut(s) 2368
AccB1I GGYRCC 1 cut(s) 881
AccB7I CCANNNNNTGG 1 cut(s) 108
AccI GTMKAC 1 cut(s) 371
AccII CGCG 1 cut(s) 385
AccIII TCCGGA 1 cut(s) 2119
AciI CCGC 5 cut(s) 385, 788, 879, 1155, 2423
AclWI GGATC 6 cut(s) 1640, 1889, 2078, 2111, 2120, 2133
AcoI YGGCCR 1 cut(s) 1540
AcsI RAATTY 5 cut(s) 193, 216, 1640, 1941, 2212
AcuI CTGAAG 4 cut(s) 146, 166, 255, 1605
AcvI CACGTG 1 cut(s) 989
AfaI GTAC 5 cut(s) 662, 1232, 1368, 2022, 2337
AfeI AGCGCT 1 cut(s) 1817
AfiI CCNNNNNNNGG 3 cut(s) 108, 602, 805
AflIII ACRYGT 3 cut(s) 986, 2009, 2149
AgeI ACCGGT 1 cut(s) 204
AhlI ACTAGT 1 cut(s) 328
AjiI CACGTC 1 cut(s) 2150
AjnI CCWGG 5 cut(s) 92, 456, 595, 937, 2001
AleI CACNNNNGTG 1 cut(s) 1390
AluBI AGCT 9 cut(s) 56, 201, 427, 1240, 1283, 1447, 1576, 1761, 2235
AluI AGCT 9 cut(s) 56, 201, 427, 1240, 1283, 1447, 1576, 1761, 2235
Alw21I GWGCWC 4 cut(s) 306, 359, 1169, 2237
Alw26I GTCTC 2 cut(s) 722, 1029
AlwI GGATC 6 cut(s) 1640, 1889, 2078, 2111, 2120, 2133
AlwNI CAGNNNCTG 1 cut(s) 779
Ama87I CYCGRG 1 cut(s) 2236
Aor13HI TCCGGA 1 cut(s) 2119
Aor51HI AGCGCT 1 cut(s) 1817
AoxI GGCC 3 cut(s) 698, 1540, 2253
ApeKI GCWGC 2 cut(s) 1283, 1505
ApoI RAATTY 5 cut(s) 193, 216, 1640, 1941, 2212
AsiGI ACCGGT 1 cut(s) 204
Asp700I GAANNNNTTC 2 cut(s) 1144, 2039
AspLEI GCGC 4 cut(s) 37, 1818, 2326, 2451
AspS9I GGNCC 3 cut(s) 698, 1794, 2353
AsuC2I CCSGG 2 cut(s) 189, 856
AsuHPI GGTGA 4 cut(s) 550, 1853, 2006, 2405
AsuII TTCGAA 1 cut(s) 168
AsuNHI GCTAGC 1 cut(s) 1357
AvaI CYCGRG 1 cut(s) 2236
AvaII GGWCC 2 cut(s) 1794, 2353
AxyI CCTNAGG 1 cut(s) 617
BaeI ACNNNNGTAYC 2 cut(s) 222, 255
BalI TGGCCA 1 cut(s) 1542
BamHI GGATCC 1 cut(s) 2125
BanI GGYRCC 1 cut(s) 881
BanII GRGCYC 1 cut(s) 2237
BarI GAAGNNNNNNTAC 2 cut(s) 2027, 2059
BauI CACGAG 2 cut(s) 1524, 1842
BbrPI CACGTG 1 cut(s) 989
BbsI GAAGAC 2 cut(s) 812, 840
Bbv12I GWGCWC 4 cut(s) 306, 359, 1169, 2237
BbvI GCAGC 2 cut(s) 1270, 1517
BccI CCATC 7 cut(s) 128, 290, 806, 1025, 1075, 1210, 1805
BciT130I CCWGG 5 cut(s) 94, 458, 597, 939, 2003
BciVI GTATCC 1 cut(s) 234
BclI TGATCA 1 cut(s) 496
BcnI CCSGG 2 cut(s) 189, 856
BcoDI GTCTC 2 cut(s) 722, 1029
BcuI ACTAGT 1 cut(s) 328
BfaI CTAG 9 cut(s) 198, 329, 446, 557, 1358, 1739, 1905, 1919, 1962
BfmI CTRYAG 2 cut(s) 504, 2221
BfoI RGCGCY 2 cut(s) 1819, 2452
BfuAI ACCTGC 1 cut(s) 2368
BfuI GTATCC 1 cut(s) 234
BglII AGATCT 2 cut(s) 1249, 1894
BisI GCNGC 2 cut(s) 1284, 1506
BlsI GCNGC 2 cut(s) 1285, 1507
Bme1390I CCNGG 7 cut(s) 94, 189, 458, 597, 856, 939, 2003
Bme18I GGWCC 2 cut(s) 1794, 2353
BmeT110I CYCGRG 1 cut(s) 2236
BmgBI CACGTC 1 cut(s) 2150
BmgT120I GGNCC 3 cut(s) 698, 1794, 2353
BmiI GGNNCC 3 cut(s) 113, 883, 2127
BmrFI CCNGG 7 cut(s) 94, 189, 458, 597, 856, 939, 2003
BmrI ACTGGG 2 cut(s) 561, 612
BmsI GCATC 4 cut(s) 650, 2300, 2408, 2428
BmtI GCTAGC 1 cut(s) 1361
BmuI ACTGGG 2 cut(s) 561, 612
BpiI GAAGAC 2 cut(s) 812, 840
Bpu10I CCTNAGC 1 cut(s) 2415
Bpu14I TTCGAA 1 cut(s) 168
BpuEI CTTGAG 1 cut(s) 1855
BpuMI CCSGG 2 cut(s) 189, 856
Bsa29I ATCGAT 2 cut(s) 1079, 2052
BsaAI YACGTR 1 cut(s) 989
BsaBI GATNNNNATC 1 cut(s) 969
BsaJI CCNNGG 6 cut(s) 93, 187, 595, 938, 1543, 1972
BsaWI WCCGGW 2 cut(s) 204, 2119
BsaXI ACNNNNNCTCC 4 cut(s) 132, 162, 315, 345
Bsc4I CCNNNNNNNGG 3 cut(s) 108, 602, 805
Bse118I RCCGGY 1 cut(s) 204
Bse1I ACTGG 4 cut(s) 548, 556, 607, 869
Bse21I CCTNAGG 1 cut(s) 617
Bse3DI GCAATG 1 cut(s) 2408
Bse8I GATNNNNATC 1 cut(s) 969
BseAI TCCGGA 1 cut(s) 2119
BseBI CCWGG 5 cut(s) 94, 458, 597, 939, 2003
BseCI ATCGAT 2 cut(s) 1079, 2052
BseDI CCNNGG 6 cut(s) 93, 187, 595, 938, 1543, 1972
BseGI GGATG 9 cut(s) 136, 466, 499, 641, 814, 998, 1734, 1884, 2419
BseJI GATNNNNATC 1 cut(s) 969
BseLI CCNNNNNNNGG 3 cut(s) 108, 602, 805
BseMI GCAATG 1 cut(s) 2408
BseMII CTCAG 1 cut(s) 242
BseNI ACTGG 4 cut(s) 548, 556, 607, 869
BseRI GAGGAG 3 cut(s) 62, 740, 2394
BseXI GCAGC 2 cut(s) 1270, 1517
BsgI GTGCAG 2 cut(s) 405, 828
Bsh1236I CGCG 1 cut(s) 385
Bsh1285I CGRYCG 1 cut(s) 389
BshFI GGCC 3 cut(s) 700, 1542, 2255
BshNI GGYRCC 1 cut(s) 881
BshTI ACCGGT 1 cut(s) 204
BshVI ATCGAT 2 cut(s) 1079, 2052
BsiEI CGRYCG 1 cut(s) 389
BsiHKAI GWGCWC 4 cut(s) 306, 359, 1169, 2237
BsiHKCI CYCGRG 1 cut(s) 2236
BsiSI CCGG 4 cut(s) 189, 205, 856, 2120
BslFI GGGAC 5 cut(s) 110, 620, 838, 997, 1597
BslI CCNNNNNNNGG 3 cut(s) 108, 602, 805
BsmAI GTCTC 2 cut(s) 722, 1029
BsmBI CGTCTC 1 cut(s) 1029
BsmFI GGGAC 5 cut(s) 110, 620, 838, 997, 1597
BsmI GAATGC 2 cut(s) 1273, 1332
BsnI GGCC 3 cut(s) 700, 1542, 2255
BsoBI CYCGRG 1 cut(s) 2236
Bsp119I TTCGAA 1 cut(s) 168
Bsp1286I GDGCHC 4 cut(s) 306, 359, 1169, 2237
Bsp13I TCCGGA 1 cut(s) 2119
Bsp1407I TGTACA 3 cut(s) 660, 1230, 2335
Bsp19I CCATGG 1 cut(s) 1543
BspACI CCGC 5 cut(s) 385, 788, 879, 1155, 2423
BspANI GGCC 3 cut(s) 700, 1542, 2255
BspCNI CTCAG 1 cut(s) 241
BspDI ATCGAT 2 cut(s) 1079, 2052
BspEI TCCGGA 1 cut(s) 2119
BspFNI CGCG 1 cut(s) 385
BspHI TCATGA 2 cut(s) 741, 2304
BspLI GGNNCC 3 cut(s) 113, 883, 2127
BspMI ACCTGC 1 cut(s) 2368
BspOI GCTAGC 1 cut(s) 1361
BspPI GGATC 6 cut(s) 1640, 1889, 2078, 2111, 2120, 2133
BspT104I TTCGAA 1 cut(s) 168
BspT107I GGYRCC 1 cut(s) 881
BsrDI GCAATG 1 cut(s) 2408
BsrFI RCCGGY 1 cut(s) 204
BsrGI TGTACA 3 cut(s) 660, 1230, 2335
BsrI ACTGG 4 cut(s) 548, 556, 607, 869
BssAI RCCGGY 1 cut(s) 204
BssECI CCNNGG 6 cut(s) 93, 187, 595, 938, 1543, 1972
BssSI CACGAG 2 cut(s) 1524, 1842
BssT1I CCWWGG 1 cut(s) 1543
Bst2BI CACGAG 2 cut(s) 1524, 1842
Bst2UI CCWGG 5 cut(s) 94, 458, 597, 939, 2003
Bst4CI ACNGT 7 cut(s) 9, 253, 775, 876, 1276, 1347, 1794
Bst6I CTCTTC 4 cut(s) 71, 80, 1581, 2432
BstAUI TGTACA 3 cut(s) 660, 1230, 2335
BstBAI YACGTR 1 cut(s) 989
BstBI TTCGAA 1 cut(s) 168
BstC8I GCNNGC 3 cut(s) 1332, 1359, 1383
BstDEI CTNAG 6 cut(s) 228, 617, 1136, 2186, 2257, 2415
BstDSI CCRYGG 2 cut(s) 1543, 1972
BstF5I GGATG 9 cut(s) 136, 466, 499, 641, 814, 998, 1734, 1884, 2419
BstFNI CGCG 1 cut(s) 385
BstH2I RGCGCY 2 cut(s) 1819, 2452
BstHHI GCGC 4 cut(s) 37, 1818, 2326, 2451
BstMAI GTCTC 2 cut(s) 722, 1029
BstMCI CGRYCG 1 cut(s) 389
BstMWI GCNNNNNNNGC 4 cut(s) 897, 1289, 1358, 1664
BstNI CCWGG 5 cut(s) 94, 458, 597, 939, 2003
BstNSI RCATGY 2 cut(s) 1750, 2013
BstSCI CCNGG 7 cut(s) 92, 187, 456, 595, 854, 937, 2001
BstSFI CTRYAG 2 cut(s) 504, 2221
BstUI CGCG 1 cut(s) 385
BstV1I GCAGC 2 cut(s) 1270, 1517
BstV2I GAAGAC 2 cut(s) 812, 840
BstX2I RGATCY 3 cut(s) 1249, 1894, 2125
BstYI RGATCY 3 cut(s) 1249, 1894, 2125
Bsu15I ATCGAT 2 cut(s) 1079, 2052
Bsu36I CCTNAGG 1 cut(s) 617
BsuI GTATCC 1 cut(s) 234
BsuRI GGCC 3 cut(s) 700, 1542, 2255
BsuTUI ATCGAT 2 cut(s) 1079, 2052
BtgI CCRYGG 2 cut(s) 1543, 1972
BtrI CACGTC 1 cut(s) 2150
BtsCI GGATG 9 cut(s) 136, 466, 499, 641, 814, 998, 1734, 1884, 2419
BtsIMutI CAGTG 4 cut(s) 5, 258, 780, 881
BveI ACCTGC 1 cut(s) 2368
Cac8I GCNNGC 3 cut(s) 1332, 1359, 1383
CaiI CAGNNNCTG 1 cut(s) 779
CciI TCATGA 2 cut(s) 741, 2304
CfoI GCGC 4 cut(s) 37, 1818, 2326, 2451
Cfr10I RCCGGY 1 cut(s) 204
Cfr13I GGNCC 3 cut(s) 698, 1794, 2353
ClaI ATCGAT 2 cut(s) 1079, 2052
Csp6I GTAC 5 cut(s) 661, 1231, 1367, 2021, 2336
CspAI ACCGGT 1 cut(s) 204
CviQI GTAC 5 cut(s) 661, 1231, 1367, 2021, 2336
DdeI CTNAG 6 cut(s) 228, 617, 1136, 2186, 2257, 2415
DraI TTTAAA 1 cut(s) 2206
DrdI GACNNNNNNGTC 1 cut(s) 2281
DseDI GACNNNNNNGTC 1 cut(s) 2281
EaeI YGGCCR 1 cut(s) 1540
Eam1104I CTCTTC 4 cut(s) 71, 80, 1581, 2432
EarI CTCTTC 4 cut(s) 71, 80, 1581, 2432
Ecl136II GAGCTC 1 cut(s) 2235
Eco130I CCWWGG 1 cut(s) 1543
Eco24I GRGCYC 1 cut(s) 2237
Eco47I GGWCC 2 cut(s) 1794, 2353
Eco47III AGCGCT 1 cut(s) 1817
Eco53kI GAGCTC 1 cut(s) 2235
Eco57I CTGAAG 4 cut(s) 146, 166, 255, 1605
Eco72I CACGTG 1 cut(s) 989
Eco81I CCTNAGG 1 cut(s) 617
Eco88I CYCGRG 1 cut(s) 2236
EcoICRI GAGCTC 1 cut(s) 2235
EcoRI GAATTC 1 cut(s) 1640
EcoRII CCWGG 5 cut(s) 92, 456, 595, 937, 2001
EcoT14I CCWWGG 1 cut(s) 1543
EcoT22I ATGCAT 1 cut(s) 2315
EcoT38I GRGCYC 1 cut(s) 2237
ErhI CCWWGG 1 cut(s) 1543
Esp3I CGTCTC 1 cut(s) 1029
FalI AAGNNNNNCTT 2 cut(s) 45, 77
FaqI GGGAC 5 cut(s) 110, 620, 838, 997, 1597
FbaI TGATCA 1 cut(s) 496
FblI GTMKAC 1 cut(s) 371
Fnu4HI GCNGC 2 cut(s) 1284, 1506
FokI GGATG 9 cut(s) 143, 473, 486, 628, 821, 1005, 1741, 1871, 2406
FriOI GRGCYC 1 cut(s) 2237
Fsp4HI GCNGC 2 cut(s) 1284, 1506
FspBI CTAG 9 cut(s) 198, 329, 446, 557, 1358, 1739, 1905, 1919, 1962
FspI TGCGCA 1 cut(s) 36
GlaI GCGC 4 cut(s) 36, 1817, 2325, 2450
GluI GCNGC 2 cut(s) 1284, 1506
HaeII RGCGCY 2 cut(s) 1819, 2452
HaeIII GGCC 3 cut(s) 700, 1542, 2255
HapII CCGG 4 cut(s) 189, 205, 856, 2120
HhaI GCGC 4 cut(s) 37, 1818, 2326, 2451
Hin6I GCGC 4 cut(s) 35, 1816, 2324, 2449
HinP1I GCGC 4 cut(s) 35, 1816, 2324, 2449
HincII GTYRAC 1 cut(s) 403
HindII GTYRAC 1 cut(s) 403
HindIII AAGCTT 1 cut(s) 1759
HinfI GANTC 8 cut(s) 89, 391, 782, 970, 1684, 1868, 2270, 2345
HpaI GTTAAC 1 cut(s) 403
HpaII CCGG 4 cut(s) 189, 205, 856, 2120
HphI GGTGA 4 cut(s) 550, 1853, 2006, 2405
Hpy166II GTNNAC 9 cut(s) 372, 403, 541, 714, 935, 1231, 1233, 2336, 2433
Hpy188I TCNGA 8 cut(s) 80, 215, 231, 636, 1303, 1899, 1948, 2100
Hpy8I GTNNAC 9 cut(s) 372, 403, 541, 714, 935, 1231, 1233, 2336, 2433
HpyAV CCTTC 6 cut(s) 121, 1042, 1172, 1298, 2139, 2238
HpyCH4III ACNGT 7 cut(s) 9, 253, 775, 876, 1276, 1347, 1794
HpyCH4IV ACGT 4 cut(s) 988, 1257, 1387, 2149
HpyF10VI GCNNNNNNNGC 4 cut(s) 897, 1289, 1358, 1664
HpyF3I CTNAG 6 cut(s) 228, 617, 1136, 2186, 2257, 2415
HpySE526I ACGT 4 cut(s) 988, 1257, 1387, 2149
HspAI GCGC 4 cut(s) 35, 1816, 2324, 2449
Kpn2I TCCGGA 1 cut(s) 2119
Ksp22I TGATCA 1 cut(s) 496
KspAI GTTAAC 1 cut(s) 403
LmnI GCTCC 2 cut(s) 1085, 2232
Lsp1109I GCAGC 2 cut(s) 1270, 1517
LweI GCATC 4 cut(s) 650, 2300, 2408, 2428
MaeI CTAG 9 cut(s) 198, 329, 446, 557, 1358, 1739, 1905, 1919, 1962
MaeII ACGT 4 cut(s) 988, 1257, 1387, 2149
MaeIII GTNAC 5 cut(s) 582, 769, 775, 860, 2012
MfeI CAATTG 1 cut(s) 702
MflI RGATCY 3 cut(s) 1249, 1894, 2125
MhlI GDGCHC 4 cut(s) 306, 359, 1169, 2237
MlsI TGGCCA 1 cut(s) 1542
MluNI TGGCCA 1 cut(s) 1542
MlyI GAGTC 1 cut(s) 1862
MmeI TCCRAC 3 cut(s) 193, 2061, 2078
Mox20I TGGCCA 1 cut(s) 1542
Mph1103I ATGCAT 1 cut(s) 2315
MroI TCCGGA 1 cut(s) 2119
MroXI GAANNNNTTC 2 cut(s) 1144, 2039
MscI TGGCCA 1 cut(s) 1542
MseI TTAA 8 cut(s) 402, 515, 569, 912, 1062, 1094, 2205, 2445
MslI CAYNNNNRTG 5 cut(s) 978, 991, 1079, 1226, 1390
Msp20I TGGCCA 1 cut(s) 1542
MspI CCGG 4 cut(s) 189, 205, 856, 2120
MspR9I CCNGG 7 cut(s) 94, 189, 458, 597, 856, 939, 2003
MunI CAATTG 1 cut(s) 702
Mva1269I GAATGC 2 cut(s) 1273, 1332
MvaI CCWGG 5 cut(s) 94, 458, 597, 939, 2003
MvnI CGCG 1 cut(s) 385
MwoI GCNNNNNNNGC 4 cut(s) 897, 1289, 1358, 1664
NciI CCSGG 2 cut(s) 189, 856
NcoI CCATGG 1 cut(s) 1543
NheI GCTAGC 1 cut(s) 1357
NlaIV GGNNCC 3 cut(s) 113, 883, 2127
NmuCI GTSAC 4 cut(s) 582, 775, 860, 2012
NsbI TGCGCA 1 cut(s) 36
NsiI ATGCAT 1 cut(s) 2315
NspI RCATGY 2 cut(s) 1750, 2013
NspV TTCGAA 1 cut(s) 168
OliI CACNNNNGTG 1 cut(s) 1390
PagI TCATGA 2 cut(s) 741, 2304
PciI ACATGT 1 cut(s) 2009
PctI GAATGC 2 cut(s) 1273, 1332
PdmI GAANNNNTTC 2 cut(s) 1144, 2039
PfeI GAWTC 7 cut(s) 89, 391, 782, 970, 1684, 2270, 2345
PflMI CCANNNNNTGG 1 cut(s) 108
PfoI TCCNGGA 1 cut(s) 456
PinAI ACCGGT 1 cut(s) 204
PkrI GCNGC 2 cut(s) 1285, 1507
PleI GAGTC 1 cut(s) 1862
PmaCI CACGTG 1 cut(s) 989
PmlI CACGTG 1 cut(s) 989
PpsI GAGTC 1 cut(s) 1862
Ppu21I YACGTR 1 cut(s) 989
PscI ACATGT 1 cut(s) 2009
PsiI TTATAA 2 cut(s) 345, 1566
Psp124BI GAGCTC 1 cut(s) 2237
Psp6I CCWGG 5 cut(s) 92, 456, 595, 937, 2001
PspCI CACGTG 1 cut(s) 989
PspGI CCWGG 5 cut(s) 92, 456, 595, 937, 2001
PspN4I GGNNCC 3 cut(s) 113, 883, 2127
PspPI GGNCC 3 cut(s) 698, 1794, 2353
PstNI CAGNNNCTG 1 cut(s) 779
PsuI RGATCY 3 cut(s) 1249, 1894, 2125
RsaI GTAC 5 cut(s) 662, 1232, 1368, 2022, 2337
RsaNI GTAC 5 cut(s) 661, 1231, 1367, 2021, 2336
RseI CAYNNNNRTG 5 cut(s) 978, 991, 1079, 1226, 1390
SacI GAGCTC 1 cut(s) 2237
SaqAI TTAA 8 cut(s) 402, 515, 569, 912, 1062, 1094, 2205, 2445
SatI GCNGC 2 cut(s) 1284, 1506
Sau96I GGNCC 3 cut(s) 698, 1794, 2353
SchI GAGTC 1 cut(s) 1862
ScrFI CCNGG 7 cut(s) 94, 189, 458, 597, 856, 939, 2003
SduI GDGCHC 4 cut(s) 306, 359, 1169, 2237
SfaNI GCATC 4 cut(s) 650, 2300, 2408, 2428
SfcI CTRYAG 2 cut(s) 504, 2221
SfuI TTCGAA 1 cut(s) 168
SinI GGWCC 2 cut(s) 1794, 2353
SmiMI CAYNNNNRTG 5 cut(s) 978, 991, 1079, 1226, 1390
SmlI CTYRAG 1 cut(s) 1870
SmoI CTYRAG 1 cut(s) 1870
SpeI ACTAGT 1 cut(s) 328
SsiI CCGC 5 cut(s) 385, 788, 879, 1155, 2423
SspI AATATT 3 cut(s) 1912, 1968, 2029
SspMI CTAG 9 cut(s) 198, 329, 446, 557, 1358, 1739, 1905, 1919, 1962
SstI GAGCTC 1 cut(s) 2237
StyD4I CCNGG 7 cut(s) 92, 187, 456, 595, 854, 937, 2001
StyI CCWWGG 1 cut(s) 1543
TaaI ACNGT 7 cut(s) 9, 253, 775, 876, 1276, 1347, 1794
TaiI ACGT 4 cut(s) 991, 1260, 1390, 2152
TaqII GACCGA 1 cut(s) 2370
TatI WGTACW 3 cut(s) 660, 1230, 2335
TfiI GAWTC 7 cut(s) 89, 391, 782, 970, 1684, 2270, 2345
Tru1I TTAA 8 cut(s) 402, 515, 569, 912, 1062, 1094, 2205, 2445
Tru9I TTAA 8 cut(s) 402, 515, 569, 912, 1062, 1094, 2205, 2445
TscAI CASTG 4 cut(s) 12, 258, 780, 881
TseFI GTSAC 4 cut(s) 582, 775, 860, 2012
TseI GCWGC 2 cut(s) 1283, 1505
Tsp45I GTSAC 4 cut(s) 582, 775, 860, 2012
TspGWI ACGGA 1 cut(s) 1989
TspRI CASTG 4 cut(s) 12, 258, 780, 881
Van91I CCANNNNNTGG 1 cut(s) 108
VpaK11BI GGWCC 2 cut(s) 1794, 2353
XapI RAATTY 5 cut(s) 193, 216, 1640, 1941, 2212
XbaI TCTAGA 1 cut(s) 1918
XceI RCATGY 2 cut(s) 1750, 2013
XcmI CCANNNNNNNNNTGG 1 cut(s) 1550
XmiI GTMKAC 1 cut(s) 371
XmnI GAANNNNTTC 2 cut(s) 1144, 2039
XspI CTAG 9 cut(s) 198, 329, 446, 557, 1358, 1739, 1905, 1919, 1962
Zsp2I ATGCAT 1 cut(s) 2315
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.