RchiOBHm_Chr5g0005161

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000112
Physical Location & Seq
Forward (+)
3348572 .. 3350707
2136 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28639

Sequence Viewer

Length: 1149 bp
ATGTTTGCTTATCGGTGGCTTATGAAAAAGAGGAAGAGAACAGGTATGAGAGTTTCATCTCCCATGAGAAGAACATGGTGGAGACAATACTCTCACTTTAGTCATTACACCGAGTCTATGGAGGAGAATAAGCTAGAGGACACAACAAACCATCCCGATTTGCAACTTTTCCATCTGAGCACGATAATAGCTGCAACAGAAGACTTCTCTCTGGCTAACAAACTAGGAGAGGGTGGATTTGGCTCTGTTTTTAAGGGTCAGTTATCCAATCAACATATGATTGCTGTGAAAAGATTGTCCATGAGTTCAAGACAAGGCATTGAAGAATTCAAAACTGAAGTTATGCTCATCGCAAAACTTCAACACAGGAATCTGGTGAAACTATTAGGATGCTGTATAGAGAAAGAAGAAAAGATGTTAATCTATGAGTATTTGCCAAACAACAGCTTGGACTCTTTTCTTTTCGATCAAACTAGAAGATCCATCTTGGATTGGAGAAAGCGGTTTGACATAGTCATTGGAGTTGCTCGTGGAATATTGTATCTTCATCAAGATTCAAGGTTGAGAATTATCCATAGGGATCTAAAAACCAGCAATGTTCTTCTAGATGCTGAAATGAAGCCAAAAGTTTCAGATTTTGGCATGGCCAGAATATTCAATGAGGATCAACAACAACATGAGACGAACAGAGTTGTTGGAACCTATGGTTATATGTCACCGGAGTATGCAGTGTATGGGAAATTTTCAACAAAGTCAGATGTATTTAGTTTTGGGGTCATATTGTTGGAGATTGTAAGTGGCAAGAAAAACAATGCTTCTTATCAGGAGGATCATTCTCGCAATTTGATAGGTCATGTTTGGGATCTATGGACAGAACAGAGAGCCTTAGACATTGTGGATTCTTCACTAAAGTCATATCATATTGATGAAGTCCTGAGATGCATTCAAATTGCGCTCCTATGCCTGCAAAAAGATGCAGCGGATAGGCCAGCTATGTCAGAAGTTGTTCTAATGTTGAGTGGTGAAAGAGCTCTTGCATCCCCTCAACGGCCAGCATTCATCTTCAAATGCAATAATCCTGATCCATTGATAGAAGAAGGATCATATTCTACTAATTATCTGACAATGACTGAAACCGTAGGTCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

382

Amino Acids

44.23

Weight (kDa)

8.44

Isoelectric Point (pI)

52.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 71 - 337 5e-52 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 71 - 336 1.2e-46 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 502, 980
AclWI GGATC 7 cut(s) 474, 588, 672, 837, 870, 1076, 1108
AcoI YGGCCR 2 cut(s) 645, 1049
AcsI RAATTY 2 cut(s) 326, 740
AcuI CTGAAG 1 cut(s) 357
AfiI CCNNNNNNNGG 1 cut(s) 1047
AgsI TTSAA 9 cut(s) 309, 323, 331, 362, 558, 658, 747, 947, 1066
AluBI AGCT 5 cut(s) 133, 191, 447, 992, 1031
AluI AGCT 5 cut(s) 133, 191, 447, 992, 1031
Alw21I GWGCWC 2 cut(s) 182, 1033
Alw26I GTCTC 2 cut(s) 76, 674
AlwI GGATC 7 cut(s) 474, 588, 672, 837, 870, 1076, 1108
AoxI GGCC 3 cut(s) 645, 986, 1049
ApeKI GCWGC 2 cut(s) 191, 977
ApoI RAATTY 2 cut(s) 326, 740
Asp700I GAANNNNTTC 1 cut(s) 1005
AspLEI GCGC 1 cut(s) 955
AsuHPI GGTGA 3 cut(s) 388, 708, 1034
BalI TGGCCA 1 cut(s) 647
BanII GRGCYC 1 cut(s) 1033
BauI CACGAG 1 cut(s) 528
BbsI GAAGAC 1 cut(s) 207
Bbv12I GWGCWC 2 cut(s) 182, 1033
BbvI GCAGC 2 cut(s) 178, 989
BccI CCATC 3 cut(s) 159, 180, 491
BceAI ACGGC 1 cut(s) 1064
BcoDI GTCTC 2 cut(s) 76, 674
BfaI CTAG 4 cut(s) 134, 224, 474, 605
BisI GCNGC 2 cut(s) 192, 978
BlsI GCNGC 2 cut(s) 193, 979
BmiI GGNNCC 1 cut(s) 700
BmsI GCATC 5 cut(s) 380, 598, 929, 964, 1046
BpiI GAAGAC 1 cut(s) 207
BsaBI GATNNNNATC 1 cut(s) 419
BsaWI WCCGGW 1 cut(s) 718
Bsc4I CCNNNNNNNGG 1 cut(s) 1047
Bse3DI GCAATG 1 cut(s) 601
Bse8I GATNNNNATC 1 cut(s) 419
BseGI GGATG 3 cut(s) 151, 395, 1037
BseJI GATNNNNATC 1 cut(s) 419
BseLI CCNNNNNNNGG 1 cut(s) 1047
BseMI GCAATG 1 cut(s) 601
BseMII CTCAG 2 cut(s) 167, 926
BseRI GAGGAG 1 cut(s) 137
BseXI GCAGC 2 cut(s) 178, 989
BshFI GGCC 3 cut(s) 647, 988, 1051
BsiHKAI GWGCWC 2 cut(s) 182, 1033
BsiSI CCGG 1 cut(s) 719
BslI CCNNNNNNNGG 1 cut(s) 1047
BsmAI GTCTC 2 cut(s) 76, 674
BsmBI CGTCTC 1 cut(s) 674
BsmI GAATGC 2 cut(s) 942, 1055
BsnI GGCC 3 cut(s) 647, 988, 1051
Bsp1286I GDGCHC 2 cut(s) 182, 1033
Bsp143I GATC 8 cut(s) 466, 479, 580, 664, 829, 862, 1081, 1100
BspACI CCGC 2 cut(s) 502, 980
BspANI GGCC 3 cut(s) 647, 988, 1051
BspCNI CTCAG 2 cut(s) 168, 927
BspLI GGNNCC 1 cut(s) 700
BspPI GGATC 7 cut(s) 474, 588, 672, 837, 870, 1076, 1108
BsrDI GCAATG 1 cut(s) 601
BssMI GATC 8 cut(s) 466, 479, 580, 664, 829, 862, 1081, 1100
BssSI CACGAG 1 cut(s) 528
Bst2BI CACGAG 1 cut(s) 528
Bst4CI ACNGT 1 cut(s) 1138
Bst6I CTCTTC 1 cut(s) 29
BstC8I GCNNGC 3 cut(s) 965, 990, 1053
BstDEI CTNAG 3 cut(s) 176, 886, 935
BstF5I GGATG 3 cut(s) 151, 395, 1037
BstHHI GCGC 1 cut(s) 955
BstKTI GATC 8 cut(s) 469, 482, 583, 667, 832, 865, 1084, 1103
BstMAI GTCTC 2 cut(s) 76, 674
BstMBI GATC 8 cut(s) 466, 479, 580, 664, 829, 862, 1081, 1100
BstV1I GCAGC 2 cut(s) 178, 989
BstV2I GAAGAC 1 cut(s) 207
BstX2I RGATCY 3 cut(s) 479, 580, 862
BstYI RGATCY 3 cut(s) 479, 580, 862
BsuRI GGCC 3 cut(s) 647, 988, 1051
BtgZI GCGATG 1 cut(s) 334
BtsCI GGATG 3 cut(s) 151, 395, 1037
BtsI GCAGTG 1 cut(s) 735
BtsIMutI CAGTG 1 cut(s) 735
Cac8I GCNNGC 3 cut(s) 965, 990, 1053
CfoI GCGC 1 cut(s) 955
CviAII CATG 6 cut(s) 64, 75, 301, 643, 677, 854
DdeI CTNAG 3 cut(s) 176, 886, 935
DpnI GATC 8 cut(s) 468, 481, 582, 666, 831, 864, 1083, 1102
DpnII GATC 8 cut(s) 466, 479, 580, 664, 829, 862, 1081, 1100
EaeI YGGCCR 2 cut(s) 645, 1049
Eam1104I CTCTTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 29
Ecl136II GAGCTC 1 cut(s) 1031
Eco24I GRGCYC 1 cut(s) 1033
Eco53kI GAGCTC 1 cut(s) 1031
Eco57I CTGAAG 1 cut(s) 357
EcoICRI GAGCTC 1 cut(s) 1031
EcoRI GAATTC 1 cut(s) 326
EcoT22I ATGCAT 1 cut(s) 944
EcoT38I GRGCYC 1 cut(s) 1033
Esp3I CGTCTC 1 cut(s) 674
FaeI CATG 6 cut(s) 67, 78, 304, 646, 680, 857
FatI CATG 6 cut(s) 63, 74, 300, 642, 676, 853
FauNDI CATATG 1 cut(s) 276
Fnu4HI GCNGC 2 cut(s) 192, 978
FokI GGATG 3 cut(s) 138, 402, 1024
FriOI GRGCYC 1 cut(s) 1033
Fsp4HI GCNGC 2 cut(s) 192, 978
FspBI CTAG 4 cut(s) 134, 224, 474, 605
GlaI GCGC 1 cut(s) 954
GluI GCNGC 2 cut(s) 192, 978
HaeIII GGCC 3 cut(s) 647, 988, 1051
HapII CCGG 1 cut(s) 719
HhaI GCGC 1 cut(s) 955
Hin1II CATG 6 cut(s) 67, 78, 304, 646, 680, 857
Hin6I GCGC 1 cut(s) 953
HinP1I GCGC 1 cut(s) 953
HinfI GANTC 5 cut(s) 113, 370, 452, 554, 899
HpaII CCGG 1 cut(s) 719
HphI GGTGA 3 cut(s) 388, 708, 1034
Hpy188I TCNGA 5 cut(s) 177, 634, 757, 1000, 1122
Hpy188III TCNNGA 7 cut(s) 155, 309, 551, 605, 824, 934, 1079
HpyAV CCTTC 1 cut(s) 1091
HpyCH4III ACNGT 1 cut(s) 1138
HpyCH4V TGCA 8 cut(s) 163, 194, 728, 942, 967, 977, 1037, 1071
HpyF3I CTNAG 3 cut(s) 176, 886, 935
Hsp92II CATG 6 cut(s) 67, 78, 304, 646, 680, 857
HspAI GCGC 1 cut(s) 953
Kzo9I GATC 8 cut(s) 466, 479, 580, 664, 829, 862, 1081, 1100
LmnI GCTCC 1 cut(s) 960
Lsp1109I GCAGC 2 cut(s) 178, 989
LweI GCATC 5 cut(s) 380, 598, 929, 964, 1046
MaeI CTAG 4 cut(s) 134, 224, 474, 605
MaeIII GTNAC 1 cut(s) 714
MalI GATC 8 cut(s) 468, 481, 582, 666, 831, 864, 1083, 1102
MboI GATC 8 cut(s) 466, 479, 580, 664, 829, 862, 1081, 1100
MflI RGATCY 3 cut(s) 479, 580, 862
MhlI GDGCHC 2 cut(s) 182, 1033
MlsI TGGCCA 1 cut(s) 647
MluCI AATT 6 cut(s) 326, 567, 740, 841, 948, 1114
MluNI TGGCCA 1 cut(s) 647
MlyI GAGTC 2 cut(s) 122, 446
MmeI TCCRAC 2 cut(s) 676, 765
MnlI CCTC 7 cut(s) 24, 115, 130, 223, 655, 820, 1053
Mox20I TGGCCA 1 cut(s) 647
Mph1103I ATGCAT 1 cut(s) 944
MroXI GAANNNNTTC 1 cut(s) 1005
MscI TGGCCA 1 cut(s) 647
MseI TTAA 2 cut(s) 252, 419
MslI CAYNNNNRTG 1 cut(s) 924
Msp20I TGGCCA 1 cut(s) 647
MspA1I CMGCKG 1 cut(s) 980
MspI CCGG 1 cut(s) 719
Mva1269I GAATGC 2 cut(s) 942, 1055
NdeI CATATG 1 cut(s) 276
NdeII GATC 8 cut(s) 466, 479, 580, 664, 829, 862, 1081, 1100
NlaIII CATG 6 cut(s) 67, 78, 304, 646, 680, 857
NlaIV GGNNCC 1 cut(s) 700
NmuCI GTSAC 1 cut(s) 714
NsiI ATGCAT 1 cut(s) 944
PctI GAATGC 2 cut(s) 942, 1055
PdmI GAANNNNTTC 1 cut(s) 1005
PfeI GAWTC 3 cut(s) 370, 554, 899
PflFI GACNNNGTC 1 cut(s) 512
PkrI GCNGC 2 cut(s) 193, 979
PleI GAGTC 2 cut(s) 121, 446
PpsI GAGTC 2 cut(s) 121, 446
Psp124BI GAGCTC 1 cut(s) 1033
PspN4I GGNNCC 1 cut(s) 700
PsuI RGATCY 3 cut(s) 479, 580, 862
PsyI GACNNNGTC 1 cut(s) 512
RseI CAYNNNNRTG 1 cut(s) 924
SacI GAGCTC 1 cut(s) 1033
SaqAI TTAA 2 cut(s) 252, 419
SatI GCNGC 2 cut(s) 192, 978
Sau3AI GATC 8 cut(s) 466, 479, 580, 664, 829, 862, 1081, 1100
SchI GAGTC 2 cut(s) 122, 446
SduI GDGCHC 2 cut(s) 182, 1033
SfaNI GCATC 5 cut(s) 380, 598, 929, 964, 1046
SmiMI CAYNNNNRTG 1 cut(s) 924
Sse9I AATT 6 cut(s) 326, 567, 740, 841, 948, 1114
SsiI CCGC 2 cut(s) 502, 980
SspI AATATT 2 cut(s) 537, 654
SspMI CTAG 4 cut(s) 134, 224, 474, 605
SstI GAGCTC 1 cut(s) 1033
TaaI ACNGT 1 cut(s) 1138
TaqI TCGA 2 cut(s) 465, 1144
TasI AATT 6 cut(s) 326, 567, 740, 841, 948, 1114
TfiI GAWTC 3 cut(s) 370, 554, 899
Tru1I TTAA 2 cut(s) 252, 419
Tru9I TTAA 2 cut(s) 252, 419
TscAI CASTG 1 cut(s) 735
TseFI GTSAC 1 cut(s) 714
TseI GCWGC 2 cut(s) 191, 977
Tsp45I GTSAC 1 cut(s) 714
TspDTI ATGAA 6 cut(s) 38, 45, 536, 632, 942, 1048
TspRI CASTG 1 cut(s) 735
Tth111I GACNNNGTC 1 cut(s) 512
XapI RAATTY 2 cut(s) 326, 740
XbaI TCTAGA 1 cut(s) 604
XmnI GAANNNNTTC 1 cut(s) 1005
XspI CTAG 4 cut(s) 134, 224, 474, 605
Zsp2I ATGCAT 1 cut(s) 944
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.