RchiOBHm_Chr4g0413561

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000112
Physical Location & Seq
Reverse (-)
37813986 .. 37816985
3000 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38406

Sequence Viewer

Length: 1083 bp
ATGCTGTATAGAGAAAGCAGAAAAGATGTTAATCTATACGAGTATTTGCCAAACAACAGCTTGGACTCTTTTCTTTTCGATAAAACTAGAAGATCCATCTTTGATTGGAGAAAGCGGTTTGACATAGTCATTGGAGTTGCTCGTGGGATATTGTATCTCCATCAAGATTCAAGGGTGAGAATTATCCGTAGGGATCTAAAAACCAGCAACGTTCTTCTAGGTGCTGAAATGAAGCCAAAAGTTTCAGATTTTGGCACCAGAATATTCAATGGGGATCAACTACAACATGAGACGAACAGAGTTTGGTTAACTTATATGTCACCGGAGTATGCAGTGTATGAGAAATTTTCAACAAAGTCAGATGTATTTAGTTTTGGGGTCATATTGTTGGAGATTGTAAGTAGCAAGAAAAACAATGATTCTTATCAGGAGGATCATTCTCGCAATTTGATAGGTCATGTTTGGGATCTATGGACAGAACAGAGAGCCTTAGACATTGTGGATTCATCACTAAAGTCATATCATATTGATGAAGACCTGAGATGCATTCAAATTGCGCTCTTATGCCTGCAAAAAGATGCAGCGGATAGGCCAACTACGCCAGAAGTTGTTCTAATGTTGAGTGCATGGATGAGAAACAGAGCTTTCAGTAACATTTTCTGGGGATTTGCAGGTGCGGCTACCGGCACCACGTGTATAGTGCGGCTGCCCAATCAAGTCTCAGAGTTTTTTGTCTTTATTTCGGAATTGTCCCTAGTTTTTAAATGTGAAATACCCAAAATATCCCTTACTTGGGTACTGATTGGTCAGCCGCACCACCACCCCAGAACCTCTCAAAGGCCAACAGCCAACTTGAGCAGAAAGATAATGTTCTGGATTAACTACGAATCCCTTGACTTAGCACAACATAATCAATGGGAACCACAACCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCAATGGGAAACAGAAGGAAGGCTAAGTCAGCAAAAGCCTCATCTTTCTTCTTTAATTGGTTGGCCTTTGACTTTGTGCAACTTTGTATTGATCATCACGACGATGTCAATTAAAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

360

Amino Acids

42.13

Weight (kDa)

9.34

Isoelectric Point (pI)

40.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 12 - 204 1e-23 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 12 - 204 8e-22 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 662
Acc36I ACCTGC 1 cut(s) 662
AccB1I GGYRCC 2 cut(s) 254, 686
AciI CCGC 5 cut(s) 115, 584, 677, 703, 812
AclI AACGTT 1 cut(s) 210
AclWI GGATC 5 cut(s) 87, 201, 282, 441, 474
AcsI RAATTY 1 cut(s) 344
AcvI CACGTG 1 cut(s) 693
AdeI CACNNNGTG 1 cut(s) 693
AfaI GTAC 1 cut(s) 798
AfiI CCNNNNNNNGG 3 cut(s) 792, 793, 837
AflIII ACRYGT 1 cut(s) 692
AgsI TTSAA 4 cut(s) 171, 268, 351, 551
AluBI AGCT 2 cut(s) 60, 644
AluI AGCT 2 cut(s) 60, 644
Alw26I GTCTC 2 cut(s) 284, 724
AlwI GGATC 5 cut(s) 87, 201, 282, 441, 474
AoxI GGCC 3 cut(s) 590, 839, 1025
ApeKI GCWGC 2 cut(s) 581, 706
ApoI RAATTY 1 cut(s) 344
Asp700I GAANNNNTTC 1 cut(s) 609
AspLEI GCGC 1 cut(s) 559
AsuHPI GGTGA 2 cut(s) 187, 312
BanI GGYRCC 2 cut(s) 254, 686
BauI CACGAG 1 cut(s) 141
BbrPI CACGTG 1 cut(s) 693
BbsI GAAGAC 1 cut(s) 540
BbvI GCAGC 2 cut(s) 593, 693
BccI CCATC 2 cut(s) 104, 168
BclI TGATCA 1 cut(s) 1053
BcoDI GTCTC 2 cut(s) 284, 724
BfaI CTAG 3 cut(s) 87, 218, 755
BfuAI ACCTGC 1 cut(s) 662
BisI GCNGC 5 cut(s) 582, 678, 704, 707, 812
BlsI GCNGC 5 cut(s) 583, 679, 705, 708, 813
BmiI GGNNCC 3 cut(s) 256, 688, 921
BmsI GCATC 2 cut(s) 533, 568
BpiI GAAGAC 1 cut(s) 540
BpuEI CTTGAG 1 cut(s) 874
BsaAI YACGTR 1 cut(s) 693
BsaBI GATNNNNATC 2 cut(s) 30, 423
BsaWI WCCGGW 1 cut(s) 322
Bsc4I CCNNNNNNNGG 3 cut(s) 792, 793, 837
Bse118I RCCGGY 1 cut(s) 683
Bse8I GATNNNNATC 2 cut(s) 30, 423
BseGI GGATG 1 cut(s) 636
BseJI GATNNNNATC 2 cut(s) 30, 423
BseLI CCNNNNNNNGG 3 cut(s) 792, 793, 837
BseMII CTCAG 2 cut(s) 530, 735
BseXI GCAGC 2 cut(s) 593, 693
BshFI GGCC 3 cut(s) 592, 841, 1027
BshNI GGYRCC 2 cut(s) 254, 686
BsiSI CCGG 2 cut(s) 323, 684
BslFI GGGAC 1 cut(s) 736
BslI CCNNNNNNNGG 3 cut(s) 792, 793, 837
BsmAI GTCTC 2 cut(s) 284, 724
BsmBI CGTCTC 1 cut(s) 284
BsmFI GGGAC 1 cut(s) 736
BsmI GAATGC 1 cut(s) 546
BsnI GGCC 3 cut(s) 592, 841, 1027
Bsp143I GATC 6 cut(s) 92, 193, 274, 433, 466, 1053
BspACI CCGC 5 cut(s) 115, 584, 677, 703, 812
BspANI GGCC 3 cut(s) 592, 841, 1027
BspCNI CTCAG 2 cut(s) 531, 734
BspLI GGNNCC 3 cut(s) 256, 688, 921
BspMI ACCTGC 1 cut(s) 662
BspPI GGATC 5 cut(s) 87, 201, 282, 441, 474
BspT107I GGYRCC 2 cut(s) 254, 686
BsrFI RCCGGY 1 cut(s) 683
BssAI RCCGGY 1 cut(s) 683
BssMI GATC 6 cut(s) 92, 193, 274, 433, 466, 1053
BssSI CACGAG 1 cut(s) 141
Bst2BI CACGAG 1 cut(s) 141
BstBAI YACGTR 1 cut(s) 693
BstC8I GCNNGC 1 cut(s) 569
BstDEI CTNAG 5 cut(s) 490, 539, 721, 898, 986
BstENI CCTNNNNNAGG 1 cut(s) 835
BstF5I GGATG 1 cut(s) 636
BstHHI GCGC 1 cut(s) 559
BstKTI GATC 6 cut(s) 95, 196, 277, 436, 469, 1056
BstMAI GTCTC 2 cut(s) 284, 724
BstMBI GATC 6 cut(s) 92, 193, 274, 433, 466, 1053
BstMWI GCNNNNNNNGC 3 cut(s) 598, 677, 991
BstV1I GCAGC 2 cut(s) 593, 693
BstV2I GAAGAC 1 cut(s) 540
BstX2I RGATCY 3 cut(s) 92, 193, 466
BstYI RGATCY 3 cut(s) 92, 193, 466
BsuRI GGCC 3 cut(s) 592, 841, 1027
BtsCI GGATG 1 cut(s) 636
BtsI GCAGTG 1 cut(s) 339
BtsIMutI CAGTG 1 cut(s) 339
BveI ACCTGC 1 cut(s) 662
Cac8I GCNNGC 1 cut(s) 569
CfoI GCGC 1 cut(s) 559
Cfr10I RCCGGY 1 cut(s) 683
Csp6I GTAC 1 cut(s) 797
CviAII CATG 3 cut(s) 287, 458, 627
CviQI GTAC 1 cut(s) 797
DdeI CTNAG 5 cut(s) 490, 539, 721, 898, 986
DpnI GATC 6 cut(s) 94, 195, 276, 435, 468, 1055
DpnII GATC 6 cut(s) 92, 193, 274, 433, 466, 1053
DraI TTTAAA 1 cut(s) 763
DraIII CACNNNGTG 1 cut(s) 693
Eco72I CACGTG 1 cut(s) 693
EcoNI CCTNNNNNAGG 1 cut(s) 835
EcoT22I ATGCAT 1 cut(s) 548
Esp3I CGTCTC 1 cut(s) 284
FaeI CATG 3 cut(s) 290, 461, 630
FaqI GGGAC 1 cut(s) 736
FatI CATG 3 cut(s) 286, 457, 626
FbaI TGATCA 1 cut(s) 1053
Fnu4HI GCNGC 5 cut(s) 582, 678, 704, 707, 812
FokI GGATG 1 cut(s) 643
Fsp4HI GCNGC 5 cut(s) 582, 678, 704, 707, 812
FspBI CTAG 3 cut(s) 87, 218, 755
GlaI GCGC 1 cut(s) 558
GluI GCNGC 5 cut(s) 582, 678, 704, 707, 812
HaeIII GGCC 3 cut(s) 592, 841, 1027
HapII CCGG 2 cut(s) 323, 684
HhaI GCGC 1 cut(s) 559
Hin1II CATG 3 cut(s) 290, 461, 630
Hin6I GCGC 1 cut(s) 557
HinP1I GCGC 1 cut(s) 557
HincII GTYRAC 1 cut(s) 309
HindII GTYRAC 1 cut(s) 309
HinfI GANTC 5 cut(s) 65, 167, 419, 503, 887
HpaI GTTAAC 1 cut(s) 309
HpaII CCGG 2 cut(s) 323, 684
HphI GGTGA 2 cut(s) 187, 312
Hpy166II GTNNAC 1 cut(s) 309
Hpy188I TCNGA 4 cut(s) 247, 361, 724, 745
Hpy188III TCNNGA 4 cut(s) 164, 428, 874, 1060
Hpy8I GTNNAC 1 cut(s) 309
Hpy99I CGWCG 1 cut(s) 1066
HpyAV CCTTC 2 cut(s) 971, 975
HpyCH4IV ACGT 2 cut(s) 210, 692
HpyCH4V TGCA 7 cut(s) 332, 546, 571, 581, 626, 671, 1041
HpyF10VI GCNNNNNNNGC 3 cut(s) 598, 677, 991
HpyF3I CTNAG 5 cut(s) 490, 539, 721, 898, 986
HpySE526I ACGT 2 cut(s) 210, 692
Hsp92II CATG 3 cut(s) 290, 461, 630
HspAI GCGC 1 cut(s) 557
Ksp22I TGATCA 1 cut(s) 1053
KspAI GTTAAC 1 cut(s) 309
Kzo9I GATC 6 cut(s) 92, 193, 274, 433, 466, 1053
Lsp1109I GCAGC 2 cut(s) 593, 693
LweI GCATC 2 cut(s) 533, 568
MaeI CTAG 3 cut(s) 87, 218, 755
MaeII ACGT 2 cut(s) 210, 692
MaeIII GTNAC 2 cut(s) 318, 650
MalI GATC 6 cut(s) 94, 195, 276, 435, 468, 1055
MboI GATC 6 cut(s) 92, 193, 274, 433, 466, 1053
MboII GAAGA 4 cut(s) 102, 206, 545, 1002
MflI RGATCY 3 cut(s) 92, 193, 466
MluCI AATT 7 cut(s) 180, 344, 445, 552, 746, 1017, 1071
MlyI GAGTC 1 cut(s) 59
MmeI TCCRAC 1 cut(s) 369
MnlI CCTC 4 cut(s) 424, 841, 939, 1011
Mph1103I ATGCAT 1 cut(s) 548
MroXI GAANNNNTTC 1 cut(s) 609
MseI TTAA 7 cut(s) 30, 308, 762, 879, 1016, 1074, 1081
MslI CAYNNNNRTG 2 cut(s) 528, 1064
MspA1I CMGCKG 1 cut(s) 584
MspI CCGG 2 cut(s) 323, 684
Mva1269I GAATGC 1 cut(s) 546
MwoI GCNNNNNNNGC 3 cut(s) 598, 677, 991
NdeII GATC 6 cut(s) 92, 193, 274, 433, 466, 1053
NlaIII CATG 3 cut(s) 290, 461, 630
NlaIV GGNNCC 3 cut(s) 256, 688, 921
NmuCI GTSAC 1 cut(s) 318
NsiI ATGCAT 1 cut(s) 548
PaqCI CACCTGC 1 cut(s) 662
PctI GAATGC 1 cut(s) 546
PdmI GAANNNNTTC 1 cut(s) 609
PfeI GAWTC 4 cut(s) 167, 419, 503, 887
PflFI GACNNNGTC 2 cut(s) 125, 1066
PkrI GCNGC 5 cut(s) 583, 679, 705, 708, 813
PleI GAGTC 1 cut(s) 59
PmaCI CACGTG 1 cut(s) 693
PmlI CACGTG 1 cut(s) 693
PpsI GAGTC 1 cut(s) 59
Ppu21I YACGTR 1 cut(s) 693
Psp1406I AACGTT 1 cut(s) 210
PspCI CACGTG 1 cut(s) 693
PspN4I GGNNCC 3 cut(s) 256, 688, 921
PsuI RGATCY 3 cut(s) 92, 193, 466
PsyI GACNNNGTC 2 cut(s) 125, 1066
RsaI GTAC 1 cut(s) 798
RsaNI GTAC 1 cut(s) 797
RseI CAYNNNNRTG 2 cut(s) 528, 1064
SaqAI TTAA 7 cut(s) 30, 308, 762, 879, 1016, 1074, 1081
SatI GCNGC 5 cut(s) 582, 678, 704, 707, 812
Sau3AI GATC 6 cut(s) 92, 193, 274, 433, 466, 1053
SchI GAGTC 1 cut(s) 59
SfaNI GCATC 2 cut(s) 533, 568
SmiMI CAYNNNNRTG 2 cut(s) 528, 1064
SmlI CTYRAG 1 cut(s) 853
SmoI CTYRAG 1 cut(s) 853
Sse9I AATT 7 cut(s) 180, 344, 445, 552, 746, 1017, 1071
SsiI CCGC 5 cut(s) 115, 584, 677, 703, 812
SspI AATATT 1 cut(s) 264
SspMI CTAG 3 cut(s) 87, 218, 755
TaiI ACGT 2 cut(s) 213, 695
TaqI TCGA 1 cut(s) 78
TasI AATT 7 cut(s) 180, 344, 445, 552, 746, 1017, 1071
TauI GCSGC 3 cut(s) 680, 706, 814
TfiI GAWTC 4 cut(s) 167, 419, 503, 887
Tru1I TTAA 7 cut(s) 30, 308, 762, 879, 1016, 1074, 1081
Tru9I TTAA 7 cut(s) 30, 308, 762, 879, 1016, 1074, 1081
TscAI CASTG 1 cut(s) 339
TseFI GTSAC 1 cut(s) 318
TseI GCWGC 2 cut(s) 581, 706
Tsp45I GTSAC 1 cut(s) 318
TspDTI ATGAA 3 cut(s) 245, 495, 546
TspGWI ACGGA 1 cut(s) 176
TspRI CASTG 1 cut(s) 339
Tth111I GACNNNGTC 2 cut(s) 125, 1066
XagI CCTNNNNNAGG 1 cut(s) 835
XapI RAATTY 1 cut(s) 344
XmnI GAANNNNTTC 1 cut(s) 609
XspI CTAG 3 cut(s) 87, 218, 755
Zsp2I ATGCAT 1 cut(s) 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.