MD05G1328200.v1.1

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000112
Physical Location & Seq
Forward (+)
45366110 .. 45372423
6314 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1328200.v1.1.491

Sequence Viewer

Length: 4104 bp
ATGGCTTGGGCCTCTGATCGTCTAATTCTCTATTGCCCTCGCAGTTGCTGTGATTGTGCCAAGGGTTTGATACATGCATCTGCCAAGAAAATGTTACTGAAAGCTTTGCTTGTTGTGTCCCTCCTCTTCCCATTTTGCACTTCCATTGACACCATAGAAATGGACCAACATGTAAAGGATGGTGATCTTCTGGTGTCCAAAGAAAACATCTTCGCATTAGGGTTCTTCGGCCCCAGAAACTCCAGCTCCAGGTACGTCGGAATTTGGTATGCAGAAAAAGATGAAAAAGATCAAAAGGCAGTGGTTTGGGTAGCAAACAGAAACGACCCCATCAATGATACCTCTGGTGTCCTAACTATAGACAGGTATGGGAGACTGCTTCTTTATTCTAACAATATGCAGAACATTCCTGTGTGGTCTACAAATGTGACGGTTCAAACTGCGAGCACTAGTTGCAGGGCTCAGCTTTTAGATTCTGGAAATTTAGTTATTTTCCGGGATAATAAAAGCAAAAACTTTATGTGGCAGAGTTTTGATTATCCTACAGATACTCAACTTCCGGGTATGAAACTAGGCGTGAATTGGAATTTGGGGATAGAATGGGTCTTAACATCTTGGAAGTCACAGGATGACCCTGGAACTGGGGAATACACCGAAAGGCTCTATTCCAATCAGACCGCCACCCCCCAGTTTTTTACTTTTTATAAAGGTTTGACTCCGTATTGGCGAGCTGGTACAGTGGACCCGCGTAATTTTGTCAGTAATCAGGACGAAATGTATTTCTTTTTTAAAAACGATAGCACTTCAATAAGGTCGGTGTTGAAGGATTCTGGGTCACTGCAAATCCTCGAGTGGAGCAATGCGAATAGAGAATGGAAGGAACTATGGTCTGCGCCAAAATATCGGTGTGACCTGTATGGAGAGTGCGGTGCCAACAGCAAATGTAGCCCTGACAATTTCAATTTTTTTGGGTGTGAGTGTCTGCCAGGGTATGAACCGAAGTCTGTCAATGATTGGAAGATGAGAAATGGTTCGGACGGGTGTGTGAGTAAACGAGTTGCGGTATCAAAGTGTAGGAACGGAGAAGGGTTCATGAAAGTGGCACAAGTTAAAGATCCAGACACAACGAAAGCAGCACGGCTGGAAAAAGGTATTAGTGTCAAAGAGTGTAAGCAGGTGTGCTTAAGCAATTGTTCTTGCACTGCATATATGGTCATAGAATCTGAAGGGCCCAGTGATTGCTTGACATGGTATGGTGAGTTGCTGGACATTTTAGTGCAGACACAGGGAGGGCGAGATCTACATGTACGTGTGGACAAAATCGAATTAGCTGAAAATTCCAGAAACTCGAAAGGTTTTCTTAGAAGGAGGGGTATGCTGGCTGTTCTGATGTTGTCTGTTCTTCTAGCATTGGTACTGATGGTTGCGTTGACCTGTTGGAGGATTAGGAAACAGAGGAAGACAAAAGTTGAGATCGAAGAAACTAGGAGACATCCCGAATGGCAATTTTTCCATCTGAGAACGATAATTGCAGCTACAAACAACTTCTCTCCAGTCCAAAAACTTGGCCAAGGTGGTTTTGGCACTGTTTATAAGGGTGTGTTACGAAATAATCAGAATATTGCTGTAAAAAGATTATCCAGAACTTCAGGACAAGGAGTTGAAGAATTCAAAAATGAAGTTGCGCTCATAGCGAGACTTCAACACAGGAATCTTGTGAAACTTTTAGGCTGTTGCATAAAGGGAGAAGAAAGGATGTTAGTCCTGGAATACTTGCCGAACAAAAGCTTGGACTACTTTCTTTTCGATCACACAAAAAAGTCCTTGCTGGATTGGCAAAAGCGATTCGAAATCATCAATGGGGTTGCTCGTGGGGTTTTGTATCTTCACCAGGATTCAAGACTTAGGATTATTCACAGGGATCTAAAAACCAGCAATGTCCTTCTAGACGCTGAGATGAACCCAAAAATCTCAGATTTCGACATGGCAAGAATCTTCCATGGGGACCAACTCCAAGATACGACCAACAGAGTTGTCGGAACATATGGCTATATGTCACCAGAGTATGCAGTGTTTGGGAGATTTTCGACCAAATCGGACGTTTTTAGTTTTGGGGTCATATTATTGGAGATTGTAAGCGGCAAGAAAAACAACGGTTTCTATCAAGCGGATCATTTCGTAAACTTAATAGGACATGTTTGGCAGTTGTGGAGTGAAGACAGAGCCTTAGAAATTGTGGAGTCATCATTGGAGTCATACGCGCCCGACGAAGCCATGAGATGCATTCAGGTCGGGCTCTTGTGTGTCGAAGAAGAGTCGAAGAACAGACCATCCATGTCAGCTGTTGTTTTTATGTTGAGCGGTGAAGCATCTGCTCCATCTCCTCAGCAGCCTGCATTTGCCTTGAGAAAAAATTCATGCGGCGATGCTGTTGTTCCGTTAGTTTCTGAAGGATCGTGTTCTATCAACGACGTGCCGGGTAAACCCTTCAAACAAGATGACCCTGGAACTGGGGAATACACCCATAGGCTCTATTCCACCCATAGGCTCTATTCCAATCAGACTGCCACCCCCGAATTTTTTACTTTTTATAAAGGTTTGACTCCGCATTGGCGATCTGAGCCAGTGGACACGGGGCGATCTGATCCAGTGGACATGGTTACTTTTGTCAGTAATCAGGACGAAATGTATTACTTTCTTAAAAACGATAGCACTTCAACAAGAATTGTGTTGAAGGATTCTGGGTTACTGCAACACCTCGGGTGGAACAATGCGGATAGAGAATGGAAGGAAATATGGTCTGCGCCAAAATATCAGTGTGACCTGTATGGAGAGTGCGGTGCCAACAGCAAATGTAGCCCTGACAATATCCATTTGTTTGGGTGTGAGTGTTTGCCAGGGTATGAACCGAAGTCTGTCAATGATTGGAAGATGAGAGATGGTTCGGACGGGTGTGTGAGTAAACGACTTGCGGTATCAAAGTGTAGGAACGGAGAAGGGTTCATGAAAGTGGCACGAGTTAAAGATCCAGACACAACCAAAGCAGCACGGCTGAAAAAAGGTATTAGTATCAAAGAGTGTGAGCAGGTGTGCTTAAGCAATTGTTCTTGCACTGCATATACGGTCATAGAATCTGAAGGGCGCGGTGATTGCTTGACATGGTATGGTGAGTTGCTGGACATTTTAGTGCACACAGAGGTAGGGCAAGATCTATATGTACGTGTGGACAAAATCGAATTAGCTGAAAATTCCAGAAATTCGAAAGGTTTTCTTAGAAGGAGGGTTGAGGTTGAAGAAACTAGGAGACATCCCGAATTGCAATTTTTCCATCTGAGAACGATAATTGCAGTCACGAACAACTTCTCTCCCGTCCAAAAACTTGGCCAAGGTGGTTTTGGCACGGTTTATAAGGGTGTGTTACAAAATAATCAGAATATTGCTGTAAAAAGATTATCCAGAACTTCAGGACAAGGAGTTGAAGAATTCAAAAATGAAGTTGCGCTTATAGCGAGACTTCAGCACAGGAATCTCGTGAAACTTTTAGGCTGTTGCATAAAGGGAGAAGAAAGGATGTTAGTCCTGGAATACTTGCCGAACAAAAGCTTGGACTACTTTCTTTTCGATCACACAAAAAAGTCCTTGTTGGATTGGCAAAAGCGATTCGAAATCATCAATGGGGTTGCTCGTGGGGTTTTGTATCTTCACCAGGACTCAAGACTTAGGATTATTCACAGGGATCTAAAAACCAGCAATGTCCTTCTAGACGCTGAGATGAACCCAAAAATCTCAGATTTCGGCATGGCAAGAATCTTCCATGGGGACCAACTCCAGGATACGACCAACAGAGTTGTCGGAACATATGGCTATATGTCACCGGAGTATGCAGTGTTTGGGAGATTTTCGACCAAATCGGATGTTTTTAGTTTTGGGGTCATATTATTGGAGATTGTAAGCAGCAAGAAAAACAACGGTTTCTATCAAGCGGATCATTTCGTAAACTTAATAGGACATGTTTGGCAGTTGTGGAGTGAAGACAGAGCCTTAGAAATTGTGGATTCGTCATTGGAGTCATACGTGCCCGACGAAGCCATGAGATGCATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

1368

Amino Acids

155.35

Weight (kDa)

8.11

Isoelectric Point (pI)

40.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 101 - 207 2.6e-34 D-mannose binding lectin
S_locus_glycop PF00954 250 - 335 2.2e-17 S-locus glycoprotein domain
PAN_2 PF08276 358 - 424 1e-16 PAN-like domain
Pkinase PF00069 518 - 719 1.1e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 520 - 783 6.2e-49 Protein tyrosine and serine/threonine kinase
S_locus_glycop PF00954 889 - 972 1.2e-17 S-locus glycoprotein domain
PAN_2 PF08276 995 - 1061 4.6e-17 PAN-like domain
Pkinase PF00069 1123 - 1320 5.8e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 1125 - 1320 3.7e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 705, 1593, 2592, 3408
AarI CACCTGC 2 cut(s) 1165, 3076
Acc36I ACCTGC 2 cut(s) 1165, 3076
AccB1I GGYRCC 2 cut(s) 929, 2840
AccBSI CCGCTC 1 cut(s) 2361
AccI GTMKAC 1 cut(s) 419
AccII CGCG 3 cut(s) 748, 2261, 3144
AclWI GGATC 8 cut(s) 1109, 1929, 2178, 2461, 2639, 3020, 3744, 3993
AcoI YGGCCR 2 cut(s) 1567, 3382
AcuI CTGAAG 6 cut(s) 1245, 1632, 2469, 3156, 3447, 3500
AfaI GTAC 5 cut(s) 254, 736, 1308, 1416, 3219
AfiI CCNNNNNNNGG 6 cut(s) 249, 641, 1440, 2510, 2544, 3829
AflII CTTAAG 2 cut(s) 1183, 3094
AflIII ACRYGT 6 cut(s) 169, 1303, 1309, 2194, 3220, 4009
AhlI ACTAGT 1 cut(s) 449
AjiI CACGTC 1 cut(s) 2473
AjuI GAANNNNNNNTTGG 6 cut(s) 572, 604, 1772, 1804, 3587, 3619
AloI GAACNNNNNNTCC 2 cut(s) 2315, 2347
Alw21I GWGCWC 2 cut(s) 449, 3192
Alw26I GTCTC 5 cut(s) 367, 1483, 1690, 3298, 3505
Alw44I GTGCAC 1 cut(s) 3188
AlwI GGATC 8 cut(s) 1109, 1929, 2178, 2461, 2639, 3020, 3744, 3993
AlwNI CAGNNNCTG 1 cut(s) 48
Ama87I CYCGRG 2 cut(s) 848, 2759
AoxI GGCC 5 cut(s) 9, 229, 1229, 1567, 3382
ApaI GGGCCC 1 cut(s) 1233
ApaLI GTGCAC 1 cut(s) 3188
ApeKI GCWGC 5 cut(s) 1133, 1532, 2389, 3044, 3954
Asp700I GAANNNNTTC 3 cut(s) 1030, 2413, 3359
AspLEI GCGC 6 cut(s) 895, 1687, 2263, 2806, 3144, 3502
AspS9I GGNCC 8 cut(s) 9, 163, 230, 742, 1229, 1230, 2005, 3820
AsuC2I CCSGG 3 cut(s) 497, 561, 2478
AsuHPI GGTGA 9 cut(s) 194, 1268, 1880, 2049, 2375, 3158, 3179, 3695, 3864
AsuII TTCGAA 3 cut(s) 1848, 3260, 3663
AvaI CYCGRG 2 cut(s) 848, 2759
AvaII GGWCC 4 cut(s) 163, 742, 2005, 3820
BaeGI GKGCMC 3 cut(s) 1233, 3192, 4080
BalI TGGCCA 2 cut(s) 1569, 3384
BanI GGYRCC 2 cut(s) 929, 2840
BanII GRGCYC 3 cut(s) 463, 1233, 2298
BauI CACGAG 4 cut(s) 1869, 3015, 3530, 3684
BbsI GAAGAC 3 cut(s) 1466, 2223, 4038
Bbv12I GWGCWC 2 cut(s) 449, 3192
BbvCI CCTCAGC 1 cut(s) 2385
BbvI GCAGC 5 cut(s) 1145, 1544, 2401, 3056, 3966
BccI CCATC 8 cut(s) 173, 338, 1414, 1521, 2338, 2386, 2933, 3336
BceAI ACGGC 2 cut(s) 1154, 3065
BcgI CGANNNNNNTGC 2 cut(s) 2272, 2306
BciVI GTATCC 1 cut(s) 3826
BcnI CCSGG 3 cut(s) 497, 561, 2478
BcoDI GTCTC 5 cut(s) 367, 1483, 1690, 3298, 3505
BcuI ACTAGT 1 cut(s) 449
BfaI CTAG 7 cut(s) 450, 572, 1406, 1485, 1946, 3300, 3761
BfmI CTRYAG 2 cut(s) 357, 543
BfrI CTTAAG 2 cut(s) 1183, 3094
BfuAI ACCTGC 2 cut(s) 1165, 3076
BfuI GTATCC 1 cut(s) 3826
BglII AGATCT 2 cut(s) 1297, 3208
BisI GCNGC 7 cut(s) 1134, 1533, 2140, 2390, 2422, 3045, 3955
BlpI GCTNAGC 1 cut(s) 462
BlsI GCNGC 7 cut(s) 1135, 1534, 2141, 2391, 2423, 3046, 3956
Bme18I GGWCC 4 cut(s) 163, 742, 2005, 3820
BmeT110I CYCGRG 2 cut(s) 848, 2759
BmgBI CACGTC 1 cut(s) 2473
BmgT120I GGNCC 8 cut(s) 9, 163, 230, 742, 1229, 1230, 2005, 3820
BmiI GGNNCC 7 cut(s) 232, 744, 931, 1231, 2006, 2842, 3821
BmrI ACTGGG 4 cut(s) 651, 682, 1227, 2520
BmsI GCATC 5 cut(s) 86, 2270, 2378, 2416, 4085
BmuI ACTGGG 4 cut(s) 651, 682, 1227, 2520
BpiI GAAGAC 3 cut(s) 1466, 2223, 4038
BpmI CTGGAG 4 cut(s) 226, 232, 1536, 3812
Bpu10I CCTNAGC 1 cut(s) 2385
Bpu1102I GCTNAGC 1 cut(s) 462
Bpu14I TTCGAA 3 cut(s) 1848, 3260, 3663
BpuEI CTTGAG 2 cut(s) 2425, 3697
BpuMI CCSGG 3 cut(s) 497, 561, 2478
BsaAI YACGTR 3 cut(s) 1310, 3221, 4075
BsaBI GATNNNNATC 1 cut(s) 183
BsaWI WCCGGW 1 cut(s) 3874
BsaXI ACNNNNNCTCC 2 cut(s) 230, 260
Bsc4I CCNNNNNNNGG 6 cut(s) 249, 641, 1440, 2510, 2544, 3829
Bse1I ACTGG 7 cut(s) 646, 688, 1233, 1553, 2515, 2624, 2648
Bse3DI GCAATG 3 cut(s) 865, 1942, 3757
Bse8I GATNNNNATC 1 cut(s) 183
BseGI GGATG 8 cut(s) 184, 634, 1492, 1761, 2330, 3307, 3576, 3919
BseJI GATNNNNATC 1 cut(s) 183
BseLI CCNNNNNNNGG 6 cut(s) 249, 641, 1440, 2510, 2544, 3829
BseMI GCAATG 3 cut(s) 865, 1942, 3757
BseMII CTCAG 9 cut(s) 476, 1508, 1944, 1986, 2399, 2610, 3323, 3759, 3801
BseNI ACTGG 7 cut(s) 646, 688, 1233, 1553, 2515, 2624, 2648
BseRI GAGGAG 2 cut(s) 113, 2373
BseSI GKGCMC 3 cut(s) 1233, 3192, 4080
BseXI GCAGC 5 cut(s) 1145, 1544, 2401, 3056, 3966
BsgI GTGCAG 1 cut(s) 1298
Bsh1236I CGCG 3 cut(s) 748, 2261, 3144
BshFI GGCC 5 cut(s) 11, 231, 1231, 1569, 3384
BshNI GGYRCC 2 cut(s) 929, 2840
BsiHKAI GWGCWC 2 cut(s) 449, 3192
BsiHKCI CYCGRG 2 cut(s) 848, 2759
BsiSI CCGG 4 cut(s) 496, 560, 2477, 3875
BslFI GGGAC 3 cut(s) 103, 2018, 3833
BslI CCNNNNNNNGG 6 cut(s) 249, 641, 1440, 2510, 2544, 3829
BsmAI GTCTC 5 cut(s) 367, 1483, 1690, 3298, 3505
BsmFI GGGAC 3 cut(s) 103, 2018, 3833
BsmI GAATGC 1 cut(s) 2283
BsnI GGCC 5 cut(s) 11, 231, 1231, 1569, 3384
BsoBI CYCGRG 2 cut(s) 848, 2759
Bsp119I TTCGAA 3 cut(s) 1848, 3260, 3663
Bsp120I GGGCCC 1 cut(s) 1229
Bsp1286I GDGCHC 6 cut(s) 449, 463, 1233, 2298, 3192, 4080
Bsp1720I GCTNAGC 1 cut(s) 462
Bsp19I CCATGG 2 cut(s) 1999, 3814
BspANI GGCC 5 cut(s) 11, 231, 1231, 1569, 3384
BspCNI CTCAG 9 cut(s) 475, 1509, 1945, 1985, 2398, 2611, 3324, 3760, 3800
BspFNI CGCG 3 cut(s) 748, 2261, 3144
BspHI TCATGA 2 cut(s) 1092, 3003
BspLI GGNNCC 7 cut(s) 232, 744, 931, 1231, 2006, 2842, 3821
BspMI ACCTGC 2 cut(s) 1165, 3076
BspPI GGATC 8 cut(s) 1109, 1929, 2178, 2461, 2639, 3020, 3744, 3993
BspT104I TTCGAA 3 cut(s) 1848, 3260, 3663
BspT107I GGYRCC 2 cut(s) 929, 2840
BspTI CTTAAG 2 cut(s) 1183, 3094
BsrBI CCGCTC 1 cut(s) 2361
BsrDI GCAATG 3 cut(s) 865, 1942, 3757
BsrI ACTGG 7 cut(s) 646, 688, 1233, 1553, 2515, 2624, 2648
BssSI CACGAG 4 cut(s) 1869, 3015, 3530, 3684
BssT1I CCWWGG 5 cut(s) 60, 1570, 1999, 3385, 3814
Bst2BI CACGAG 4 cut(s) 1869, 3015, 3530, 3684
Bst4CI ACNGT 7 cut(s) 433, 739, 1588, 2156, 3124, 3403, 3971
Bst6I CTCTTC 2 cut(s) 131, 2307
BstAFI CTTAAG 2 cut(s) 1183, 3094
BstAPI GCANNNNNTGC 1 cut(s) 453
BstBAI YACGTR 3 cut(s) 1310, 3221, 4075
BstBI TTCGAA 3 cut(s) 1848, 3260, 3663
BstC8I GCNNGC 4 cut(s) 445, 729, 1380, 2394
BstDSI CCRYGG 2 cut(s) 1999, 3814
BstENI CCTNNNNNAGG 1 cut(s) 1438
BstF5I GGATG 8 cut(s) 184, 634, 1492, 1761, 2330, 3307, 3576, 3919
BstFNI CGCG 3 cut(s) 748, 2261, 3144
BstHHI GCGC 6 cut(s) 895, 1687, 2263, 2806, 3144, 3502
BstMAI GTCTC 5 cut(s) 367, 1483, 1690, 3298, 3505
BstMWI GCNNNNNNNGC 8 cut(s) 453, 945, 1691, 1834, 2620, 2856, 3150, 3506
BstNSI RCATGY 5 cut(s) 77, 173, 1307, 2198, 4013
BstSFI CTRYAG 2 cut(s) 357, 543
BstSLI GKGCMC 3 cut(s) 1233, 3192, 4080
BstUI CGCG 3 cut(s) 748, 2261, 3144
BstV1I GCAGC 5 cut(s) 1145, 1544, 2401, 3056, 3966
BstV2I GAAGAC 3 cut(s) 1466, 2223, 4038
BstX2I RGATCY 6 cut(s) 1114, 1297, 1921, 3025, 3208, 3736
BstXI CCANNNNNNTGG 4 cut(s) 160, 1565, 2879, 3380
BstYI RGATCY 6 cut(s) 1114, 1297, 1921, 3025, 3208, 3736
BsuI GTATCC 1 cut(s) 3826
BsuRI GGCC 5 cut(s) 11, 231, 1231, 1569, 3384
BtgI CCRYGG 2 cut(s) 1999, 3814
BtgZI GCGATG 1 cut(s) 2439
BtrI CACGTC 1 cut(s) 2473
BtsCI GGATG 8 cut(s) 184, 634, 1492, 1761, 2330, 3307, 3576, 3919
BtsI GCAGTG 6 cut(s) 306, 836, 1200, 2076, 3111, 3891
BveI ACCTGC 2 cut(s) 1165, 3076
Cac8I GCNNGC 4 cut(s) 445, 729, 1380, 2394
CaiI CAGNNNCTG 1 cut(s) 48
CciI TCATGA 2 cut(s) 1092, 3003
CfoI GCGC 6 cut(s) 895, 1687, 2263, 2806, 3144, 3502
Cfr13I GGNCC 8 cut(s) 9, 163, 230, 742, 1229, 1230, 2005, 3820
CseI GACGC 2 cut(s) 1958, 3773
Csp6I GTAC 5 cut(s) 253, 735, 1307, 1415, 3218
CviQI GTAC 5 cut(s) 253, 735, 1307, 1415, 3218
DraI TTTAAA 1 cut(s) 790
EaeI YGGCCR 2 cut(s) 1567, 3382
Eam1104I CTCTTC 2 cut(s) 131, 2307
EarI CTCTTC 2 cut(s) 131, 2307
Eco130I CCWWGG 5 cut(s) 60, 1570, 1999, 3385, 3814
Eco24I GRGCYC 3 cut(s) 463, 1233, 2298
Eco47I GGWCC 4 cut(s) 163, 742, 2005, 3820
Eco57I CTGAAG 6 cut(s) 1245, 1632, 2469, 3156, 3447, 3500
Eco88I CYCGRG 2 cut(s) 848, 2759
EcoNI CCTNNNNNAGG 1 cut(s) 1438
EcoO109I RGGNCCY 1 cut(s) 1229
EcoRI GAATTC 2 cut(s) 1667, 3482
EcoT14I CCWWGG 5 cut(s) 60, 1570, 1999, 3385, 3814
EcoT22I ATGCAT 3 cut(s) 79, 2285, 4100
EcoT38I GRGCYC 3 cut(s) 463, 1233, 2298
ErhI CCWWGG 5 cut(s) 60, 1570, 1999, 3385, 3814
FalI AAGNNNNNCTT 8 cut(s) 93, 125, 1344, 1376, 3255, 3287, 3486, 3518
FaqI GGGAC 3 cut(s) 103, 2018, 3833
FauI CCCGC 1 cut(s) 753
FauNDI CATATG 2 cut(s) 2044, 3859
FblI GTMKAC 1 cut(s) 419
Fnu4HI GCNGC 7 cut(s) 1134, 1533, 2140, 2390, 2422, 3045, 3955
FokI GGATG 8 cut(s) 191, 641, 1479, 1768, 2317, 3294, 3583, 3926
FriOI GRGCYC 3 cut(s) 463, 1233, 2298
Fsp4HI GCNGC 7 cut(s) 1134, 1533, 2140, 2390, 2422, 3045, 3955
FspBI CTAG 7 cut(s) 450, 572, 1406, 1485, 1946, 3300, 3761
GlaI GCGC 6 cut(s) 894, 1686, 2262, 2805, 3143, 3501
GluI GCNGC 7 cut(s) 1134, 1533, 2140, 2390, 2422, 3045, 3955
GsuI CTGGAG 4 cut(s) 226, 232, 1536, 3812
HaeIII GGCC 5 cut(s) 11, 231, 1231, 1569, 3384
HapII CCGG 4 cut(s) 496, 560, 2477, 3875
HgaI GACGC 2 cut(s) 1958, 3773
HhaI GCGC 6 cut(s) 895, 1687, 2263, 2806, 3144, 3502
Hin6I GCGC 6 cut(s) 893, 1685, 2261, 2804, 3142, 3500
HinP1I GCGC 6 cut(s) 893, 1685, 2261, 2804, 3142, 3500
HincII GTYRAC 1 cut(s) 1431
HindII GTYRAC 1 cut(s) 1431
HindIII AAGCTT 3 cut(s) 102, 1786, 3601
HpaII CCGG 4 cut(s) 496, 560, 2477, 3875
HphI GGTGA 9 cut(s) 194, 1268, 1880, 2049, 2375, 3158, 3179, 3695, 3864
Hpy99I CGWCG 4 cut(s) 260, 2270, 2474, 4085
HpyCH4III ACNGT 7 cut(s) 433, 739, 1588, 2156, 3124, 3403, 3971
HpyCH4IV ACGT 6 cut(s) 255, 1309, 2100, 2472, 3220, 4074
HpyF10VI GCNNNNNNNGC 8 cut(s) 453, 945, 1691, 1834, 2620, 2856, 3150, 3506
HpySE526I ACGT 6 cut(s) 255, 1309, 2100, 2472, 3220, 4074
HspAI GCGC 6 cut(s) 893, 1685, 2261, 2804, 3142, 3500
LmnI GCTCC 3 cut(s) 251, 855, 2380
Lsp1109I GCAGC 5 cut(s) 1145, 1544, 2401, 3056, 3966
LweI GCATC 5 cut(s) 86, 2270, 2378, 2416, 4085
MaeI CTAG 7 cut(s) 450, 572, 1406, 1485, 1946, 3300, 3761
MaeII ACGT 6 cut(s) 255, 1309, 2100, 2472, 3220, 4074
MbiI CCGCTC 1 cut(s) 2361
MfeI CAATTG 2 cut(s) 1189, 3100
MflI RGATCY 6 cut(s) 1114, 1297, 1921, 3025, 3208, 3736
MhlI GDGCHC 6 cut(s) 449, 463, 1233, 2298, 3192, 4080
MlsI TGGCCA 2 cut(s) 1569, 3384
MluNI TGGCCA 2 cut(s) 1569, 3384
MlyI GAGTC 7 cut(s) 709, 2249, 2261, 2324, 2596, 3704, 4076
MmeI TCCRAC 5 cut(s) 238, 1418, 2017, 3624, 3832
Mox20I TGGCCA 2 cut(s) 1569, 3384
Mph1103I ATGCAT 3 cut(s) 79, 2285, 4100
MroXI GAANNNNTTC 3 cut(s) 1030, 2413, 3359
MscI TGGCCA 2 cut(s) 1569, 3384
MseI TTAA 9 cut(s) 608, 789, 1110, 1184, 2186, 2700, 3021, 3095, 4001
MslI CAYNNNNRTG 7 cut(s) 158, 410, 1097, 1274, 1308, 3008, 3185
Msp20I TGGCCA 2 cut(s) 1569, 3384
MspA1I CMGCKG 1 cut(s) 2342
MspCI CTTAAG 2 cut(s) 1183, 3094
MspI CCGG 4 cut(s) 496, 560, 2477, 3875
MunI CAATTG 2 cut(s) 1189, 3100
Mva1269I GAATGC 1 cut(s) 2283
MvnI CGCG 3 cut(s) 748, 2261, 3144
MwoI GCNNNNNNNGC 8 cut(s) 453, 945, 1691, 1834, 2620, 2856, 3150, 3506
NciI CCSGG 3 cut(s) 497, 561, 2478
NcoI CCATGG 2 cut(s) 1999, 3814
NdeI CATATG 2 cut(s) 2044, 3859
NlaIV GGNNCC 7 cut(s) 232, 744, 931, 1231, 2006, 2842, 3821
NmuCI GTSAC 8 cut(s) 427, 621, 834, 908, 2055, 2819, 3349, 3870
NsiI ATGCAT 3 cut(s) 79, 2285, 4100
NspI RCATGY 5 cut(s) 77, 173, 1307, 2198, 4013
NspV TTCGAA 3 cut(s) 1848, 3260, 3663
PaeR7I CTCGAG 1 cut(s) 848
PagI TCATGA 2 cut(s) 1092, 3003
PaqCI CACCTGC 2 cut(s) 1165, 3076
PciI ACATGT 4 cut(s) 169, 1303, 2194, 4009
PcsI WCGNNNNNNNCGW 2 cut(s) 2265, 4080
PctI GAATGC 1 cut(s) 2283
PdmI GAANNNNTTC 3 cut(s) 1030, 2413, 3359
PfoI TCCNGGA 4 cut(s) 495, 1764, 3579, 3828
PkrI GCNGC 7 cut(s) 1135, 1534, 2141, 2391, 2423, 3046, 3956
PleI GAGTC 7 cut(s) 709, 2248, 2260, 2323, 2596, 3704, 4075
PpsI GAGTC 7 cut(s) 709, 2248, 2260, 2323, 2596, 3704, 4075
Ppu21I YACGTR 3 cut(s) 1310, 3221, 4075
PscI ACATGT 4 cut(s) 169, 1303, 2194, 4009
PsiI TTATAA 4 cut(s) 705, 1593, 2592, 3408
PspN4I GGNNCC 7 cut(s) 232, 744, 931, 1231, 2006, 2842, 3821
PspOMI GGGCCC 1 cut(s) 1229
PspPI GGNCC 8 cut(s) 9, 163, 230, 742, 1229, 1230, 2005, 3820
PspXI VCTCGAGB 1 cut(s) 848
PstNI CAGNNNCTG 1 cut(s) 48
PsuI RGATCY 6 cut(s) 1114, 1297, 1921, 3025, 3208, 3736
PvuII CAGCTG 1 cut(s) 2342
RsaI GTAC 5 cut(s) 254, 736, 1308, 1416, 3219
RsaNI GTAC 5 cut(s) 253, 735, 1307, 1415, 3218
RseI CAYNNNNRTG 7 cut(s) 158, 410, 1097, 1274, 1308, 3008, 3185
SaqAI TTAA 9 cut(s) 608, 789, 1110, 1184, 2186, 2700, 3021, 3095, 4001
SatI GCNGC 7 cut(s) 1134, 1533, 2140, 2390, 2422, 3045, 3955
Sau96I GGNCC 8 cut(s) 9, 163, 230, 742, 1229, 1230, 2005, 3820
SchI GAGTC 7 cut(s) 709, 2249, 2261, 2324, 2596, 3704, 4076
SduI GDGCHC 6 cut(s) 449, 463, 1233, 2298, 3192, 4080
SfaNI GCATC 5 cut(s) 86, 2270, 2378, 2416, 4085
SfcI CTRYAG 2 cut(s) 357, 543
Sfr274I CTCGAG 1 cut(s) 848
SfuI TTCGAA 3 cut(s) 1848, 3260, 3663
SinI GGWCC 4 cut(s) 163, 742, 2005, 3820
SlaI CTCGAG 1 cut(s) 848
SmiMI CAYNNNNRTG 7 cut(s) 158, 410, 1097, 1274, 1308, 3008, 3185
SmlI CTYRAG 5 cut(s) 848, 1183, 2404, 3094, 3712
SmoI CTYRAG 5 cut(s) 848, 1183, 2404, 3094, 3712
SpeI ACTAGT 1 cut(s) 449
SspI AATATT 2 cut(s) 1621, 3436
SspMI CTAG 7 cut(s) 450, 572, 1406, 1485, 1946, 3300, 3761
StyI CCWWGG 5 cut(s) 60, 1570, 1999, 3385, 3814
TaaI ACNGT 7 cut(s) 433, 739, 1588, 2156, 3124, 3403, 3971
TaiI ACGT 6 cut(s) 258, 1312, 2103, 2475, 3223, 4077
TauI GCSGC 2 cut(s) 2142, 2424
Tru1I TTAA 9 cut(s) 608, 789, 1110, 1184, 2186, 2700, 3021, 3095, 4001
Tru9I TTAA 9 cut(s) 608, 789, 1110, 1184, 2186, 2700, 3021, 3095, 4001
TseFI GTSAC 8 cut(s) 427, 621, 834, 908, 2055, 2819, 3349, 3870
TseI GCWGC 5 cut(s) 1133, 1532, 2389, 3044, 3954
Tsp45I GTSAC 8 cut(s) 427, 621, 834, 908, 2055, 2819, 3349, 3870
TspGWI ACGGA 4 cut(s) 708, 1095, 2427, 3006
Vha464I CTTAAG 2 cut(s) 1183, 3094
VneI GTGCAC 1 cut(s) 3188
VpaK11BI GGWCC 4 cut(s) 163, 742, 2005, 3820
XagI CCTNNNNNAGG 1 cut(s) 1438
XbaI TCTAGA 2 cut(s) 1945, 3760
XceI RCATGY 5 cut(s) 77, 173, 1307, 2198, 4013
XcmI CCANNNNNNNNNTGG 2 cut(s) 1577, 3392
XhoI CTCGAG 1 cut(s) 848
XmiI GTMKAC 1 cut(s) 419
XmnI GAANNNNTTC 3 cut(s) 1030, 2413, 3359
XspI CTAG 7 cut(s) 450, 572, 1406, 1485, 1946, 3300, 3761
Zsp2I ATGCAT 3 cut(s) 79, 2285, 4100
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.