Rroxscaffold_2G00113530

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000112
Physical Location & Seq
Reverse (-)
39213820 .. 39220700
6881 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00113530.1

Sequence Viewer

Length: 2097 bp
ATGCTATTACTCGGGCTCCTCAACAAATCCACCTCGATCAATCACTTTCTTTCCGATCATTCGGTGCCCGCGCACACCAGCTTGGAATTTTCTTGGTTTCATGCTCAATGCGCCCACCAAAAGGGTCATAGAGATGATATGCTGCAATCGGTACAGTTCTGCACTTCCAGGGACGCCATAAAAGGGAATGAAGAACAATTGAAGGATGTTGATGGAGACTATTTAGCGTCCAAAGAAAGCAAGTTTGAATTGGGTTTCTTCAGCCCCGGAAATTCTAGCTACAGGTATGTTGGAATTTGGTATTCTCAAACTAGAGTATCCACTAAAACAGTGGTGTGGGTTGCAAACAGGAACAATCCCATCAATGATACCTCTGGTGTGCTCACAATAAACAGATATGGAAAACTAGCCCTTTATGCTTATAACATGGAGAGCACTCCTATTTGGTCTATTAATGTGTCGCGGTCGGTTCAAAATGCTAACACAAGCACTTTATCTGCACAGCTTTTAGATACAGGAAATTTAGTTGTGTTCCAGGCTGATAATAATGAAATCCATGTATGGCAAAGTTTTGATTATCCTACAGATACTTTAATTCCAGGTATGAAAGTTGGGGTGAATTGGAAAACCGGAATGGAATGGATTTTAACATCTTGGAAGTCACAAGATGACCCTGGAACTGGGGACTATACCTTCAGGCTCTATCCATATCAGATCGCATCCCCCCAATTTTTTATTTACAAGGGTTTAAGTAAGTATTACCGAACTCATCCAGGGCCAACGCCTGGTTTGGTCACTAGTCAAGAGGAAACTTATTATTTCATGAATGAAACCAATGCAATTACAAGAGTAACAGTGACTAATTCTTTGTTGGAGAACCTTCATTGGGATGATGATGGCCTTCAATGGAAGGTAGACTTCTCTGCACCGAAGTCCCGGTGTGACGGGTATGGAAAGTGTGGTGCCAACAGCAGATGTAGCCCTGACAACGTTAGTGTGTTTGAGTGTGAATGTTTATCAGGGTATGTGCCTAATTCTATAAGTGATTGGAATCAGAAAAATGGGTCAGGTGGATGTGTGAGTAATCGACTTGGTTTGTTGAAGTGTGGAGATGGAGACGGGTTTGTGAAGGTGGCAAGAGTTAAATATCCAGACACATCGATAGCAGCATGGTTAAAAGCAGGAATGAGTGCCAAAGAGTGTGAGCAGGAGTGCCTAAGAAATTGTTCTTGCAAAGCATATTTGAGCACTGAAAATGAAGGGATTGTTGATTGCTTGACATGGTATGATGACTTGATGGACATTTTAACGTACACAGAGTTTGGACAAGATCTCTACGTTCGTGTGAATGCAACCGTGTTAGCTGCAAATGCCGGAAGATCACAAGGTTTCTTGGAAAGGAGGGGTATGCCGGCCATTCCAATACTGTCTGCTGTGCTGGCATTGGTACTAATCATTATGCTTCTCTATTGGTGGCGTAAGAAGAACAGGAACACAAAAGGCATAGATTTTGTGGAGGCAGAGGAGCTTGAGGAAACTCAGAGACACCCCGAATTGCAATTTTTCGATCTTGACACAATAATAGCAGCCACAGACCACTTCTCTCATGTCAATGAACTTGGCCGTGGTGGTTTTGGCTCTGTTTATAAGGGTCAGTTACCAAATGGACAGGAGATTGCTGTGAAAAGATTGTCCAAAACTTCAGGACAAGGGATGGAGGAATTCAAAAATGAAGTTGCACTTATAGCAGGACTTCAACACAGGAATCTTGTGAAACTTTTAGGCTGTTGTATAAAGGGAGAAGAAAGGATGTTAGTCCTAGAATACATGCCTAACAAAAGCTTGGACTCCTTTCTGTTTGATAACAGAAGACGGTCCTTGGATTGGAAAAAGCGATTTGAAATTATCAACGGGATTGCTCGTGGGATTCTGTATCTTCACCAAGACTCAAGATTGAGGATTATCCATAGAGATCTAAAAACTAGCAATGTTCTACTAGATGATGAGATGAACCCAAAAGTTTCTGATTTTGGCATGGCTAGAATATTCCGCGGGGACAAACTGCAATATAAGACGAACCGAATTGTCGGAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

698

Amino Acids

78.96

Weight (kDa)

6.46

Isoelectric Point (pI)

32.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 110 - 220 3.3e-34 D-mannose binding lectin
S_locus_glycop PF00954 269 - 345 2.6e-13 S-locus glycoprotein domain
PAN_2 PF08276 369 - 435 3.2e-17 PAN-like domain
Pkinase PF00069 535 - 695 2.1e-32 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 537 - 691 2e-35 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 423, 1647
AccB1I GGYRCC 2 cut(s) 64, 962
AccB7I CCANNNNNTGG 1 cut(s) 785
AccI GTMKAC 1 cut(s) 915
AccII CGCG 3 cut(s) 71, 463, 2052
AciI CCGC 4 cut(s) 69, 463, 2050, 2052
AclI AACGTT 1 cut(s) 990
AcoI YGGCCR 2 cut(s) 1413, 1621
AcsI RAATTY 5 cut(s) 86, 271, 294, 520, 1721
AcuI CTGAAG 3 cut(s) 244, 679, 1686
AcyI GRCGYC 1 cut(s) 174
AfaI GTAC 3 cut(s) 153, 1313, 1449
AfiI CCNNNNNNNGG 6 cut(s) 121, 183, 680, 785, 886, 1884
AgsI TTSAA 8 cut(s) 202, 248, 473, 905, 1102, 1726, 1757, 1901
AhlI ACTAGT 1 cut(s) 797
AjnI CCWGG 6 cut(s) 167, 534, 598, 673, 772, 784
AluBI AGCT 6 cut(s) 81, 279, 505, 1364, 1528, 1842
AluI AGCT 6 cut(s) 81, 279, 505, 1364, 1528, 1842
Alw21I GWGCWC 3 cut(s) 384, 437, 1250
Alw26I GTCTC 3 cut(s) 210, 1110, 1537
Ama87I CYCGRG 1 cut(s) 11
AoxI GGCC 4 cut(s) 776, 898, 1413, 1621
ApeKI GCWGC 4 cut(s) 142, 1166, 1364, 1586
ApoI RAATTY 5 cut(s) 86, 271, 294, 520, 1721
ArsI GACNNNNNNTTYG 2 cut(s) 850, 882
AseI ATTAAT 1 cut(s) 453
Asp700I GAANNNNTTC 1 cut(s) 1225
AspLEI GCGC 2 cut(s) 73, 113
AspS9I GGNCC 2 cut(s) 776, 1875
AsuC2I CCSGG 2 cut(s) 267, 937
AsuHPI GGTGA 2 cut(s) 628, 1931
AvaI CYCGRG 1 cut(s) 11
AvaII GGWCC 1 cut(s) 1875
BaeGI GKGCMC 1 cut(s) 69
BaeI ACNNNNGTAYC 2 cut(s) 300, 333
BanI GGYRCC 2 cut(s) 64, 962
BanII GRGCYC 1 cut(s) 18
BauI CACGAG 1 cut(s) 1920
BbsI GAAGAC 1 cut(s) 1876
Bbv12I GWGCWC 3 cut(s) 384, 437, 1250
BbvI GCAGC 4 cut(s) 129, 1178, 1351, 1598
BccI CCATC 6 cut(s) 206, 368, 890, 1106, 1291, 1708
BceAI ACGGC 1 cut(s) 1608
BciT130I CCWGG 6 cut(s) 169, 536, 600, 675, 774, 786
BciVI GTATCC 1 cut(s) 328
BcnI CCSGG 2 cut(s) 267, 937
BcoDI GTCTC 3 cut(s) 210, 1110, 1537
BcuI ACTAGT 1 cut(s) 797
BfaI CTAG 8 cut(s) 276, 312, 407, 798, 1820, 1983, 1997, 2040
BfmI CTRYAG 2 cut(s) 280, 582
BfuI GTATCC 1 cut(s) 328
BglII AGATCT 2 cut(s) 1330, 1972
BisI GCNGC 4 cut(s) 143, 1167, 1365, 1587
BlsI GCNGC 4 cut(s) 144, 1168, 1366, 1588
Bme1390I CCNGG 8 cut(s) 169, 267, 536, 600, 675, 774, 786, 937
Bme18I GGWCC 1 cut(s) 1875
BmeT110I CYCGRG 1 cut(s) 11
BmgT120I GGNCC 2 cut(s) 776, 1875
BmiI GGNNCC 3 cut(s) 17, 66, 964
BmrFI CCNGG 8 cut(s) 169, 267, 536, 600, 675, 774, 786, 937
BmrI ACTGGG 1 cut(s) 690
BmsI GCATC 1 cut(s) 728
BmuI ACTGGG 1 cut(s) 690
BpiI GAAGAC 1 cut(s) 1876
BpuEI CTTGAG 2 cut(s) 1550, 1933
BpuMI CCSGG 2 cut(s) 267, 937
Bsa29I ATCGAT 1 cut(s) 1160
BsaBI GATNNNNATC 1 cut(s) 1050
BsaHI GRCGYC 1 cut(s) 174
BsaJI CCNNGG 7 cut(s) 168, 265, 673, 773, 1624, 1878, 2050
BsaWI WCCGGW 1 cut(s) 629
BsaXI ACNNNNNCTCC 1 cut(s) 30
Bsc4I CCNNNNNNNGG 6 cut(s) 121, 183, 680, 785, 886, 1884
Bse118I RCCGGY 1 cut(s) 1411
Bse1I ACTGG 1 cut(s) 685
Bse3DI GCAATG 1 cut(s) 1993
Bse8I GATNNNNATC 1 cut(s) 1050
BseBI CCWGG 6 cut(s) 169, 536, 600, 675, 774, 786
BseCI ATCGAT 1 cut(s) 1160
BseDI CCNNGG 7 cut(s) 168, 265, 673, 773, 1624, 1878, 2050
BseGI GGATG 7 cut(s) 211, 719, 769, 895, 1079, 1719, 1815
BseJI GATNNNNATC 1 cut(s) 1050
BseLI CCNNNNNNNGG 6 cut(s) 121, 183, 680, 785, 886, 1884
BseMI GCAATG 1 cut(s) 1993
BseMII CTCAG 1 cut(s) 1553
BseNI ACTGG 1 cut(s) 685
BseRI GAGGAG 2 cut(s) 8, 1538
BseSI GKGCMC 1 cut(s) 69
BseXI GCAGC 4 cut(s) 129, 1178, 1351, 1598
BsgI GTGCAG 3 cut(s) 145, 483, 909
Bsh1236I CGCG 3 cut(s) 71, 463, 2052
Bsh1285I CGRYCG 1 cut(s) 467
BshFI GGCC 4 cut(s) 778, 900, 1415, 1623
BshNI GGYRCC 2 cut(s) 64, 962
BshVI ATCGAT 1 cut(s) 1160
BsiEI CGRYCG 1 cut(s) 467
BsiHKAI GWGCWC 3 cut(s) 384, 437, 1250
BsiHKCI CYCGRG 1 cut(s) 11
BsiSI CCGG 5 cut(s) 267, 630, 937, 1374, 1412
BslFI GGGAC 4 cut(s) 185, 698, 919, 2069
BslI CCNNNNNNNGG 6 cut(s) 121, 183, 680, 785, 886, 1884
BsmAI GTCTC 3 cut(s) 210, 1110, 1537
BsmBI CGTCTC 1 cut(s) 1110
BsmFI GGGAC 4 cut(s) 185, 698, 919, 2069
BsmI GAATGC 1 cut(s) 1354
BsnI GGCC 4 cut(s) 778, 900, 1415, 1623
BsoBI CYCGRG 1 cut(s) 11
Bsp1286I GDGCHC 5 cut(s) 18, 69, 384, 437, 1250
Bsp143I GATC 7 cut(s) 36, 55, 714, 1330, 1379, 1567, 1972
BspACI CCGC 4 cut(s) 69, 463, 2050, 2052
BspANI GGCC 4 cut(s) 778, 900, 1415, 1623
BspCNI CTCAG 1 cut(s) 1552
BspDI ATCGAT 1 cut(s) 1160
BspFNI CGCG 3 cut(s) 71, 463, 2052
BspHI TCATGA 1 cut(s) 822
BspLI GGNNCC 3 cut(s) 17, 66, 964
BspT107I GGYRCC 2 cut(s) 64, 962
BsrDI GCAATG 1 cut(s) 1993
BsrFI RCCGGY 1 cut(s) 1411
BsrI ACTGG 1 cut(s) 685
BssAI RCCGGY 1 cut(s) 1411
BssECI CCNNGG 7 cut(s) 168, 265, 673, 773, 1624, 1878, 2050
BssMI GATC 7 cut(s) 36, 55, 714, 1330, 1379, 1567, 1972
BssNI GRCGYC 1 cut(s) 174
BssSI CACGAG 1 cut(s) 1920
BssT1I CCWWGG 1 cut(s) 1878
Bst2BI CACGAG 1 cut(s) 1920
Bst2UI CCWGG 6 cut(s) 169, 536, 600, 675, 774, 786
Bst4CI ACNGT 6 cut(s) 156, 331, 856, 1357, 1428, 1875
BstACI GRCGYC 1 cut(s) 174
BstC8I GCNNGC 3 cut(s) 69, 1413, 1440
BstDEI CTNAG 2 cut(s) 1217, 1539
BstDSI CCRYGG 2 cut(s) 1624, 2050
BstF5I GGATG 7 cut(s) 211, 719, 769, 895, 1079, 1719, 1815
BstFNI CGCG 3 cut(s) 71, 463, 2052
BstHHI GCGC 2 cut(s) 73, 113
BstKTI GATC 7 cut(s) 39, 58, 717, 1333, 1382, 1570, 1975
BstMAI GTCTC 3 cut(s) 210, 1110, 1537
BstMBI GATC 7 cut(s) 36, 55, 714, 1330, 1379, 1567, 1972
BstMCI CGRYCG 1 cut(s) 467
BstMWI GCNNNNNNNGC 6 cut(s) 110, 416, 978, 1370, 1439, 1745
BstNI CCWGG 6 cut(s) 169, 536, 600, 675, 774, 786
BstNSI RCATGY 1 cut(s) 1831
BstSCI CCNGG 8 cut(s) 167, 265, 534, 598, 673, 772, 784, 935
BstSFI CTRYAG 2 cut(s) 280, 582
BstSLI GKGCMC 1 cut(s) 69
BstUI CGCG 3 cut(s) 71, 463, 2052
BstV1I GCAGC 4 cut(s) 129, 1178, 1351, 1598
BstV2I GAAGAC 1 cut(s) 1876
BstX2I RGATCY 2 cut(s) 1330, 1972
BstYI RGATCY 2 cut(s) 1330, 1972
Bsu15I ATCGAT 1 cut(s) 1160
BsuI GTATCC 1 cut(s) 328
BsuRI GGCC 4 cut(s) 778, 900, 1415, 1623
BsuTUI ATCGAT 1 cut(s) 1160
BtgI CCRYGG 2 cut(s) 1624, 2050
BtsCI GGATG 7 cut(s) 211, 719, 769, 895, 1079, 1719, 1815
BtsIMutI CAGTG 3 cut(s) 336, 861, 1248
Cac8I GCNNGC 3 cut(s) 69, 1413, 1440
CciI TCATGA 1 cut(s) 822
CfoI GCGC 2 cut(s) 73, 113
Cfr10I RCCGGY 1 cut(s) 1411
Cfr13I GGNCC 2 cut(s) 776, 1875
Cfr42I CCGCGG 1 cut(s) 2053
ClaI ATCGAT 1 cut(s) 1160
CseI GACGC 2 cut(s) 182, 216
Csp6I GTAC 3 cut(s) 152, 1312, 1448
CviAII CATG 9 cut(s) 101, 427, 557, 823, 1170, 1281, 1607, 1828, 2035
CviQI GTAC 3 cut(s) 152, 1312, 1448
DdeI CTNAG 2 cut(s) 1217, 1539
DpnI GATC 7 cut(s) 38, 57, 716, 1332, 1381, 1569, 1974
DpnII GATC 7 cut(s) 36, 55, 714, 1330, 1379, 1567, 1972
EaeI YGGCCR 2 cut(s) 1413, 1621
Eco130I CCWWGG 1 cut(s) 1878
Eco24I GRGCYC 1 cut(s) 18
Eco47I GGWCC 1 cut(s) 1875
Eco57I CTGAAG 3 cut(s) 244, 679, 1686
Eco88I CYCGRG 1 cut(s) 11
EcoRI GAATTC 1 cut(s) 1721
EcoRII CCWGG 6 cut(s) 167, 534, 598, 673, 772, 784
EcoT14I CCWWGG 1 cut(s) 1878
EcoT38I GRGCYC 1 cut(s) 18
ErhI CCWWGG 1 cut(s) 1878
Esp3I CGTCTC 1 cut(s) 1110
FaeI CATG 9 cut(s) 104, 430, 560, 826, 1173, 1284, 1610, 1831, 2038
FalI AAGNNNNNCTT 4 cut(s) 902, 934, 1725, 1757
FaqI GGGAC 4 cut(s) 185, 698, 919, 2069
FatI CATG 9 cut(s) 100, 426, 556, 822, 1169, 1280, 1606, 1827, 2034
FauI CCCGC 2 cut(s) 76, 2045
FblI GTMKAC 1 cut(s) 915
Fnu4HI GCNGC 4 cut(s) 143, 1167, 1365, 1587
FokI GGATG 7 cut(s) 218, 706, 756, 902, 1086, 1726, 1822
FriOI GRGCYC 1 cut(s) 18
Fsp4HI GCNGC 4 cut(s) 143, 1167, 1365, 1587
FspBI CTAG 8 cut(s) 276, 312, 407, 798, 1820, 1983, 1997, 2040
GlaI GCGC 2 cut(s) 72, 112
GluI GCNGC 4 cut(s) 143, 1167, 1365, 1587
HaeIII GGCC 4 cut(s) 778, 900, 1415, 1623
HapII CCGG 5 cut(s) 267, 630, 937, 1374, 1412
HgaI GACGC 2 cut(s) 182, 216
HhaI GCGC 2 cut(s) 73, 113
Hin1I GRCGYC 1 cut(s) 174
Hin1II CATG 9 cut(s) 104, 430, 560, 826, 1173, 1284, 1610, 1831, 2038
Hin6I GCGC 2 cut(s) 71, 111
HinP1I GCGC 2 cut(s) 71, 111
HindIII AAGCTT 1 cut(s) 1840
HinfI GANTC 5 cut(s) 1051, 1765, 1847, 1927, 1946
HpaII CCGG 5 cut(s) 267, 630, 937, 1374, 1412
HphI GGTGA 2 cut(s) 628, 1931
Hpy166II GTNNAC 2 cut(s) 916, 1314
Hpy188I TCNGA 6 cut(s) 55, 714, 1056, 1542, 2026, 2090
Hpy188III TCNNGA 6 cut(s) 803, 823, 1151, 1571, 1704, 1950
Hpy8I GTNNAC 2 cut(s) 916, 1314
HpyAV CCTTC 7 cut(s) 196, 703, 890, 904, 911, 1123, 1253
HpyCH4III ACNGT 6 cut(s) 156, 331, 856, 1357, 1428, 1875
HpyCH4IV ACGT 3 cut(s) 990, 1310, 1338
HpyF10VI GCNNNNNNNGC 6 cut(s) 110, 416, 978, 1370, 1439, 1745
HpyF3I CTNAG 2 cut(s) 1217, 1539
HpySE526I ACGT 3 cut(s) 990, 1310, 1338
Hsp92I GRCGYC 1 cut(s) 174
Hsp92II CATG 9 cut(s) 104, 430, 560, 826, 1173, 1284, 1610, 1831, 2038
HspAI GCGC 2 cut(s) 71, 111
KroI GCCGGC 1 cut(s) 1411
KroNI GCCGGC 1 cut(s) 1413
KspI CCGCGG 1 cut(s) 2053
Kzo9I GATC 7 cut(s) 36, 55, 714, 1330, 1379, 1567, 1972
LmnI GCTCC 2 cut(s) 21, 1525
Lsp1109I GCAGC 4 cut(s) 129, 1178, 1351, 1598
LweI GCATC 1 cut(s) 728
MaeI CTAG 8 cut(s) 276, 312, 407, 798, 1820, 1983, 1997, 2040
MaeII ACGT 3 cut(s) 990, 1310, 1338
MaeIII GTNAC 6 cut(s) 660, 793, 850, 856, 941, 1656
MalI GATC 7 cut(s) 38, 57, 716, 1332, 1381, 1569, 1974
MboI GATC 7 cut(s) 36, 55, 714, 1330, 1379, 1567, 1972
MboII GAAGA 7 cut(s) 203, 250, 1389, 1495, 1814, 1881, 1928
MfeI CAATTG 1 cut(s) 197
MflI RGATCY 2 cut(s) 1330, 1972
MhlI GDGCHC 5 cut(s) 18, 69, 384, 437, 1250
MlyI GAGTC 2 cut(s) 1841, 1940
MmeI TCCRAC 3 cut(s) 271, 852, 2068
MroNI GCCGGC 1 cut(s) 1411
MroXI GAANNNNTTC 1 cut(s) 1225
MseI TTAA 7 cut(s) 453, 593, 647, 749, 1143, 1175, 1307
MslI CAYNNNNRTG 3 cut(s) 132, 888, 1611
MspA1I CMGCKG 1 cut(s) 2052
MspI CCGG 5 cut(s) 267, 630, 937, 1374, 1412
MspR9I CCNGG 8 cut(s) 169, 267, 536, 600, 675, 774, 786, 937
MunI CAATTG 1 cut(s) 197
Mva1269I GAATGC 1 cut(s) 1354
MvaI CCWGG 6 cut(s) 169, 536, 600, 675, 774, 786
MvnI CGCG 3 cut(s) 71, 463, 2052
MwoI GCNNNNNNNGC 6 cut(s) 110, 416, 978, 1370, 1439, 1745
NaeI GCCGGC 1 cut(s) 1413
NciI CCSGG 2 cut(s) 267, 937
NdeII GATC 7 cut(s) 36, 55, 714, 1330, 1379, 1567, 1972
NgoMIV GCCGGC 1 cut(s) 1411
NlaIII CATG 9 cut(s) 104, 430, 560, 826, 1173, 1284, 1610, 1831, 2038
NlaIV GGNNCC 3 cut(s) 17, 66, 964
NmuCI GTSAC 4 cut(s) 660, 793, 856, 941
NspI RCATGY 1 cut(s) 1831
PagI TCATGA 1 cut(s) 822
PctI GAATGC 1 cut(s) 1354
PdiI GCCGGC 1 cut(s) 1413
PdmI GAANNNNTTC 1 cut(s) 1225
PfeI GAWTC 3 cut(s) 1051, 1765, 1927
PflMI CCANNNNNTGG 1 cut(s) 785
PkrI GCNGC 4 cut(s) 144, 1168, 1366, 1588
PleI GAGTC 2 cut(s) 1841, 1940
PpsI GAGTC 2 cut(s) 1841, 1940
PshBI ATTAAT 1 cut(s) 453
PsiI TTATAA 2 cut(s) 423, 1647
Psp1406I AACGTT 1 cut(s) 990
Psp6I CCWGG 6 cut(s) 167, 534, 598, 673, 772, 784
PspGI CCWGG 6 cut(s) 167, 534, 598, 673, 772, 784
PspN4I GGNNCC 3 cut(s) 17, 66, 964
PspPI GGNCC 2 cut(s) 776, 1875
PsuI RGATCY 2 cut(s) 1330, 1972
RsaI GTAC 3 cut(s) 153, 1313, 1449
RsaNI GTAC 3 cut(s) 152, 1312, 1448
RseI CAYNNNNRTG 3 cut(s) 132, 888, 1611
SacII CCGCGG 1 cut(s) 2053
SaqAI TTAA 7 cut(s) 453, 593, 647, 749, 1143, 1175, 1307
SatI GCNGC 4 cut(s) 143, 1167, 1365, 1587
Sau3AI GATC 7 cut(s) 36, 55, 714, 1330, 1379, 1567, 1972
Sau96I GGNCC 2 cut(s) 776, 1875
SchI GAGTC 2 cut(s) 1841, 1940
ScrFI CCNGG 8 cut(s) 169, 267, 536, 600, 675, 774, 786, 937
SduI GDGCHC 5 cut(s) 18, 69, 384, 437, 1250
SfaNI GCATC 1 cut(s) 728
SfcI CTRYAG 2 cut(s) 280, 582
Sfr303I CCGCGG 1 cut(s) 2053
SgrBI CCGCGG 1 cut(s) 2053
SinI GGWCC 1 cut(s) 1875
SmiMI CAYNNNNRTG 3 cut(s) 132, 888, 1611
SmlI CTYRAG 2 cut(s) 1529, 1948
SmoI CTYRAG 2 cut(s) 1529, 1948
SpeI ACTAGT 1 cut(s) 797
SsiI CCGC 4 cut(s) 69, 463, 2050, 2052
SspI AATATT 1 cut(s) 2046
SspMI CTAG 8 cut(s) 276, 312, 407, 798, 1820, 1983, 1997, 2040
StyD4I CCNGG 8 cut(s) 167, 265, 534, 598, 673, 772, 784, 935
StyI CCWWGG 1 cut(s) 1878
TaaI ACNGT 6 cut(s) 156, 331, 856, 1357, 1428, 1875
TaiI ACGT 3 cut(s) 993, 1313, 1341
TaqI TCGA 4 cut(s) 35, 1087, 1160, 1566
TfiI GAWTC 3 cut(s) 1051, 1765, 1927
Tru1I TTAA 7 cut(s) 453, 593, 647, 749, 1143, 1175, 1307
Tru9I TTAA 7 cut(s) 453, 593, 647, 749, 1143, 1175, 1307
TscAI CASTG 3 cut(s) 336, 861, 1255
TseFI GTSAC 4 cut(s) 660, 793, 856, 941
TseI GCWGC 4 cut(s) 142, 1166, 1364, 1586
Tsp45I GTSAC 4 cut(s) 660, 793, 856, 941
TspRI CASTG 3 cut(s) 336, 861, 1255
Van91I CCANNNNNTGG 1 cut(s) 785
VpaK11BI GGWCC 1 cut(s) 1875
VspI ATTAAT 1 cut(s) 453
XapI RAATTY 5 cut(s) 86, 271, 294, 520, 1721
XceI RCATGY 1 cut(s) 1831
XcmI CCANNNNNNNNNTGG 1 cut(s) 328
XmiI GTMKAC 1 cut(s) 915
XmnI GAANNNNTTC 1 cut(s) 1225
XspI CTAG 8 cut(s) 276, 312, 407, 798, 1820, 1983, 1997, 2040
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.