RchiOBHm_Chr3g0476811

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000112
Physical Location & Seq
Forward (+)
22776956 .. 22780239
3284 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44220

Sequence Viewer

Length: 2532 bp
ATGAAAAAGGATCTCTGGTCATTTTTTATATTGACACATAGCCAGATTGCAAACGCCCTGAAAATGCTGTTGAAATCTCTGCTTCTCTTCCTCCTCTTCCAAGTTTGCACTTCCAGGGACACCTTAAGATCGAACGAAGAACTGAAGGATGATGATGGTGGACTTCTGGTGTCCAAAGAAAGCAGCTTTGAGTTGGGTTTCTTCAGCCCTGGAAATTCTAGCAACCGCTATGTTGGGATTTGGTATTCTCAATCTAAAGTATCTGAGAAAACAGTGGTGTGGATCGCAAACAGAGACAACCCCATCAATGATACCTCTGGAGTTTTCACCATAAACAGGTATGGAGAGCTAGTTCTTTATGCTTATAAAATGGAGACCAGTCCTATATGGTCTGCAGATGCGTCTCTTTCAGTCAAAAACACCAGCACCTTATCTGCATATGTACAGCTTTTGGATACAGGAAACTTAGTTATGTTCCAGAATGATAGAGAAGGAATCTTTGTATGGCAAAGTTTTGATTATCCTACAGATACTTTAATCCCTGGTATGAAAGTTGGGATGAATCGGAAAACTGGGCTAGAATGGGTTTTAACATCTTGGAAGTCACTAGATGACCCTGGAACTGGGGACTATACCTTTAGGCTTCATAATTGGGGCCACATTTTCACACCCCAGTCTTTTATTTATAAAGGTTTGTCTAAGTATTGGCGAGGTGATACAGAGCCTTGGCCTAATTCGATCCGAAATGCAGAGGAAATTTCTTTTTTCCTTTCTTATAACACCAATAAACTTACAAAAATGAAGTTGAAGTTTTCTGGGCTACATCAGCACTTGGTATGGAGTGATGCTGACCATCAATGGAAGGAGTTGTGGTCTTCACCGAAATACAGGTGTGACTGGTATGGACATTGTGGTGCCAACAGCAAATGTAGCCTTGACAGTGGTAATCTGTTCGAGTGTGAGTGTCTACCGGGGTATGAACCAAAATCTATAAATGGTTGGAATGGGAGAGATGGGTCAGATGGATGTGTGAGTAAAAGAGTCAAAGTGTCAAAGTGCAGAAATGGAGAAGGGTTTGTGAAGGTAGAAAGGGTGAAAAATCCAGACGCATCGAGAGCAGCATTGTTGAAAACAGGTATGACTACCAAAGAGTGCGAGCAGGAATGCCTAAGAAATTGTTCATGCACGGCATATATGACCAAAAAGATTGAAGGGGTTATTAATTGCTTGACATGGTATGATGACTTGATGGACATTTTGGTTTACACAGAGGACGGACGAGATCTGTATGTTCGTGTGGATAAAATTGAGTTAGGTACTATATTTTTTGCCAACGAAAGTTTCAAAGATTTTGTGAGTTCTCTTTACTGGACTAGGTTTTGTGTCCATAATGCCGCAAACTCACAAGGATTCTTCAGGAGGGGCATGCTTGTTGTCCCAATACTGTTTGCTCTGCTGGCCCTAGTACTAATAAATGTGCTTGCCTGTTGGTTGCTTAAGAAAAAAGGGAAGACAAAAGTCTTTGTGGATGTGGATGAACTTGAGGAAAGCAAGAGACACCCGGAATTGCAATTTTTCGATCTGAACACACTAGTAGCAGCCACAGATAATTTCTCTGATGTTAATAAACTTGGCGAAGGTGGTTTTGGTGAAGGTTGCTGTAAAAGGCTGTCTAGCAGCTCAGGACAAGGGATTGGTGAATTCAAAAATGAAGTTGAACTGATAGCAGGACTTCAACACAGGAATCTTGTGAAGCTTTTAGGTTGTTGTATAAAGGGAGGAGAAAGGATGCTAGTCCCCGAATATATGCCGAACAAAAGCTTGGACTCATTTCTTTTTGATAACACAAGAAGGTCCTTCTTAGATTGGGGAAGGCGTTTTGAAATTATCAATGGGGTTTCTCGTGGGCTTTTGTATCTTCACCAAGACTCGAGATTAAGGATTATTCATAGGGATCTAAAAACCAGTAATATTCTTCTAGATGATGAGATGACCCCAAAAATTTCAGATTTCGGCATGGCAAGAATATTCCATGGGGACCAACTTCAGGATAACACAAAGAAAGTTGTTGGAACATATGGCTATATGTCACCAGAGTATGCAGTGTTTGGGAGATTCTCTACGAAATCGGATGTGTTTAGTTTTGGGATTATACTATTGGAGATTGTTAGTGGCAAGAGAAACAATGGTTCTTATGAGGAGAATTACTCCATGAACCTGATAGGACATGTTTGGCATGTGTGGAAAGAAGGAATAGCCTTAGATATTGTGGATTCATCGCAACAATCATATAAGCATGATGAAGTTTTGAGATGCATTCAAGTTGGACTGTTGTGCGTGCAAGAAGATTCAAAGGACCGGCCAGCCATGTCATCCATTGTGTTCATGTTGAGTGGTGAGGTATCTCTTCCATCTCCTAAGCAGCCTGCATTTGTCTTCCGAAGAAATTCCAGCAATGCTGCTGATTGCTCAAGTCCAAAAGAATTGCATTCTATTGACGACTTGACAATAACTATTGTGGGAGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

843

Amino Acids

95.71

Weight (kDa)

6.79

Isoelectric Point (pI)

41.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 91 - 201 5.7e-35 D-mannose binding lectin
S_locus_glycop PF00954 250 - 330 2.2e-15 S-locus glycoprotein domain
PAN_2 PF08276 353 - 419 2.8e-18 PAN-like domain
PK_Tyr_Ser-Thr PF07714 560 - 795 3.1e-39 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 566 - 793 2e-35 Protein kinase domain
DUF3403 PF11883 800 - 843 5.5e-07 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 366, 687, 777
AccB1I GGYRCC 1 cut(s) 914
AccI GTMKAC 1 cut(s) 967
AciI CCGC 2 cut(s) 226, 1395
AclWI GGATC 4 cut(s) 18, 290, 733, 1962
AcoI YGGCCR 1 cut(s) 2360
AcsI RAATTY 5 cut(s) 214, 756, 1700, 2001, 2446
AcuI CTGAAG 4 cut(s) 164, 187, 1399, 2030
AfaI GTAC 3 cut(s) 444, 1318, 1467
AfiI CCNNNNNNNGG 3 cut(s) 336, 623, 2047
AflII CTTAAG 2 cut(s) 124, 1496
AflIII ACRYGT 1 cut(s) 2227
AhlI ACTAGT 1 cut(s) 1591
AjnI CCWGG 4 cut(s) 113, 208, 541, 616
AjuI GAANNNNNNNTTGG 6 cut(s) 1325, 1357, 1629, 1661, 1805, 1837
AloI GAACNNNNNNTCC 2 cut(s) 336, 368
AluBI AGCT 7 cut(s) 186, 349, 448, 1680, 1756, 1821, 2525
AluI AGCT 7 cut(s) 186, 349, 448, 1680, 1756, 1821, 2525
Alw21I GWGCWC 1 cut(s) 2527
Alw26I GTCTC 4 cut(s) 288, 368, 408, 1549
AlwI GGATC 4 cut(s) 18, 290, 733, 1962
Ama87I CYCGRG 1 cut(s) 1930
AoxI GGCC 4 cut(s) 655, 728, 1458, 2360
ApeKI GCWGC 6 cut(s) 183, 1118, 1598, 1677, 2422, 2459
ApoI RAATTY 5 cut(s) 214, 756, 1700, 2001, 2446
AseI ATTAAT 1 cut(s) 1221
Asp700I GAANNNNTTC 2 cut(s) 1177, 2446
AspS9I GGNCC 5 cut(s) 655, 1459, 1854, 2038, 2356
AsuC2I CCSGG 2 cut(s) 972, 1562
AsuHPI GGTGA 9 cut(s) 319, 725, 870, 1105, 1661, 1709, 1913, 2082, 2408
AvaI CYCGRG 1 cut(s) 1930
AvaII GGWCC 3 cut(s) 1854, 2038, 2356
BanI GGYRCC 1 cut(s) 914
BanII GRGCYC 1 cut(s) 2527
BauI CACGAG 1 cut(s) 1902
BbsI GAAGAC 3 cut(s) 867, 1517, 2428
Bbv12I GWGCWC 1 cut(s) 2527
BbvI GCAGC 6 cut(s) 195, 1130, 1610, 1689, 2434, 2446
BccI CCATC 7 cut(s) 149, 311, 861, 1007, 1016, 1243, 2419
BceAI ACGGC 1 cut(s) 1203
BciT130I CCWGG 4 cut(s) 115, 210, 543, 618
BciVI GTATCC 1 cut(s) 448
BcnI CCSGG 2 cut(s) 972, 1562
BcoDI GTCTC 4 cut(s) 288, 368, 408, 1549
BcuI ACTAGT 1 cut(s) 1591
BfmI CTRYAG 2 cut(s) 393, 525
BfrI CTTAAG 2 cut(s) 124, 1496
BfuI GTATCC 1 cut(s) 448
BglII AGATCT 1 cut(s) 1282
BisI GCNGC 7 cut(s) 184, 1119, 1395, 1599, 1678, 2423, 2460
BlsI GCNGC 7 cut(s) 185, 1120, 1396, 1600, 1679, 2424, 2461
BmcAI AGTACT 1 cut(s) 1467
Bme1390I CCNGG 6 cut(s) 115, 210, 543, 618, 972, 1562
Bme18I GGWCC 3 cut(s) 1854, 2038, 2356
BmeT110I CYCGRG 1 cut(s) 1930
BmgT120I GGNCC 5 cut(s) 655, 1459, 1854, 2038, 2356
BmiI GGNNCC 3 cut(s) 656, 916, 2039
BmrFI CCNGG 6 cut(s) 115, 210, 543, 618, 972, 1562
BmrI ACTGGG 3 cut(s) 582, 633, 667
BmsI GCATC 5 cut(s) 388, 835, 1118, 1779, 2303
BmuI ACTGGG 3 cut(s) 582, 633, 667
BoxI GACNNNNGTC 1 cut(s) 1517
BpiI GAAGAC 3 cut(s) 867, 1517, 2428
BplI GAGNNNNNCTC 2 cut(s) 2192, 2224
BpmI CTGGAG 1 cut(s) 339
Bpu10I CCTNAGC 2 cut(s) 1681, 2418
BpuEI CTTGAG 2 cut(s) 1562, 2455
BpuMI CCSGG 2 cut(s) 972, 1562
BsaI GGTCTC 1 cut(s) 368
BsaJI CCNNGG 7 cut(s) 114, 208, 541, 616, 725, 971, 2032
BsaXI ACNNNNNCTCC 4 cut(s) 336, 366, 857, 887
Bsc4I CCNNNNNNNGG 3 cut(s) 336, 623, 2047
Bse118I RCCGGY 1 cut(s) 2358
Bse1I ACTGG 7 cut(s) 378, 577, 628, 673, 902, 1373, 1965
Bse3DI GCAATG 1 cut(s) 2461
BseBI CCWGG 4 cut(s) 115, 210, 543, 618
BseDI CCNNGG 7 cut(s) 114, 208, 541, 616, 725, 971, 2032
BseGI GGATG 8 cut(s) 154, 564, 1031, 1534, 1540, 1794, 2137, 2372
BseLI CCNNNNNNNGG 3 cut(s) 336, 623, 2047
BseMI GCAATG 1 cut(s) 2461
BseMII CTCAG 2 cut(s) 255, 1695
BseNI ACTGG 7 cut(s) 378, 577, 628, 673, 902, 1373, 1965
BseRI GAGGAG 3 cut(s) 83, 1794, 2213
BseXI GCAGC 6 cut(s) 195, 1130, 1610, 1689, 2434, 2446
BsgI GTGCAG 1 cut(s) 1078
BshFI GGCC 4 cut(s) 657, 730, 1460, 2362
BshNI GGYRCC 1 cut(s) 914
BsiHKAI GWGCWC 1 cut(s) 2527
BsiHKCI CYCGRG 1 cut(s) 1930
BsiSI CCGG 3 cut(s) 971, 1562, 2359
BslFI GGGAC 5 cut(s) 131, 641, 1421, 1781, 2051
BslI CCNNNNNNNGG 3 cut(s) 336, 623, 2047
BsmAI GTCTC 4 cut(s) 288, 368, 408, 1549
BsmBI CGTCTC 1 cut(s) 408
BsmFI GGGAC 5 cut(s) 131, 641, 1421, 1781, 2051
BsmI GAATGC 3 cut(s) 1169, 2316, 2488
BsnI GGCC 4 cut(s) 657, 730, 1460, 2362
Bso31I GGTCTC 1 cut(s) 368
BsoBI CYCGRG 1 cut(s) 1930
Bsp1286I GDGCHC 1 cut(s) 2527
Bsp1407I TGTACA 1 cut(s) 442
Bsp143I GATC 7 cut(s) 10, 128, 282, 738, 1282, 1579, 1954
Bsp19I CCATGG 1 cut(s) 2032
BspACI CCGC 2 cut(s) 226, 1395
BspANI GGCC 4 cut(s) 657, 730, 1460, 2362
BspCNI CTCAG 2 cut(s) 256, 1694
BspLI GGNNCC 3 cut(s) 656, 916, 2039
BspMAI CTGCAG 1 cut(s) 397
BspPI GGATC 4 cut(s) 18, 290, 733, 1962
BspT107I GGYRCC 1 cut(s) 914
BspTI CTTAAG 2 cut(s) 124, 1496
BspTNI GGTCTC 1 cut(s) 368
BsrDI GCAATG 1 cut(s) 2461
BsrFI RCCGGY 1 cut(s) 2358
BsrGI TGTACA 1 cut(s) 442
BsrI ACTGG 7 cut(s) 378, 577, 628, 673, 902, 1373, 1965
BssAI RCCGGY 1 cut(s) 2358
BssECI CCNNGG 7 cut(s) 114, 208, 541, 616, 725, 971, 2032
BssMI GATC 7 cut(s) 10, 128, 282, 738, 1282, 1579, 1954
BssSI CACGAG 1 cut(s) 1902
BssT1I CCWWGG 2 cut(s) 725, 2032
Bst2BI CACGAG 1 cut(s) 1902
Bst2UI CCWGG 4 cut(s) 115, 210, 543, 618
Bst4CI ACNGT 4 cut(s) 274, 941, 1446, 2331
Bst6I CTCTTC 3 cut(s) 92, 101, 2412
BstAFI CTTAAG 2 cut(s) 124, 1496
BstAUI TGTACA 1 cut(s) 442
BstC8I GCNNGC 7 cut(s) 1157, 1427, 1458, 1482, 2339, 2364, 2427
BstDEI CTNAG 8 cut(s) 264, 466, 699, 1169, 1681, 1861, 2260, 2418
BstDSI CCRYGG 1 cut(s) 2032
BstF5I GGATG 8 cut(s) 154, 564, 1031, 1534, 1540, 1794, 2137, 2372
BstKTI GATC 7 cut(s) 13, 131, 285, 741, 1285, 1582, 1957
BstMAI GTCTC 4 cut(s) 288, 368, 408, 1549
BstMBI GATC 7 cut(s) 10, 128, 282, 738, 1282, 1579, 1954
BstMWI GCNNNNNNNGC 4 cut(s) 826, 930, 1115, 1457
BstNI CCWGG 4 cut(s) 115, 210, 543, 618
BstNSI RCATGY 3 cut(s) 1429, 2231, 2240
BstPAI GACNNNNGTC 1 cut(s) 1517
BstSCI CCNGG 6 cut(s) 113, 208, 541, 616, 970, 1560
BstSFI CTRYAG 2 cut(s) 393, 525
BstV1I GCAGC 6 cut(s) 195, 1130, 1610, 1689, 2434, 2446
BstV2I GAAGAC 3 cut(s) 867, 1517, 2428
BstX2I RGATCY 3 cut(s) 10, 1282, 1954
BstYI RGATCY 3 cut(s) 10, 1282, 1954
BsuI GTATCC 1 cut(s) 448
BsuRI GGCC 4 cut(s) 657, 730, 1460, 2362
BtgI CCRYGG 1 cut(s) 2032
BtgZI GCGATG 1 cut(s) 2262
BtsCI GGATG 8 cut(s) 154, 564, 1031, 1534, 1540, 1794, 2137, 2372
BtsI GCAGTG 1 cut(s) 2109
BtsIMutI CAGTG 3 cut(s) 279, 946, 2109
Cac8I GCNNGC 7 cut(s) 1157, 1427, 1458, 1482, 2339, 2364, 2427
Cfr10I RCCGGY 1 cut(s) 2358
Cfr13I GGNCC 5 cut(s) 655, 1459, 1854, 2038, 2356
CseI GACGC 2 cut(s) 390, 1115
Csp6I GTAC 3 cut(s) 443, 1317, 1466
CviQI GTAC 3 cut(s) 443, 1317, 1466
DdeI CTNAG 8 cut(s) 264, 466, 699, 1169, 1681, 1861, 2260, 2418
DpnI GATC 7 cut(s) 12, 130, 284, 740, 1284, 1581, 1956
DpnII GATC 7 cut(s) 10, 128, 282, 738, 1282, 1579, 1954
EaeI YGGCCR 1 cut(s) 2360
Eam1104I CTCTTC 3 cut(s) 92, 101, 2412
EarI CTCTTC 3 cut(s) 92, 101, 2412
Ecl136II GAGCTC 1 cut(s) 2525
Eco130I CCWWGG 2 cut(s) 725, 2032
Eco24I GRGCYC 1 cut(s) 2527
Eco31I GGTCTC 1 cut(s) 368
Eco47I GGWCC 3 cut(s) 1854, 2038, 2356
Eco53kI GAGCTC 1 cut(s) 2525
Eco57I CTGAAG 4 cut(s) 164, 187, 1399, 2030
Eco88I CYCGRG 1 cut(s) 1930
EcoICRI GAGCTC 1 cut(s) 2525
EcoO109I RGGNCCY 1 cut(s) 1854
EcoRI GAATTC 1 cut(s) 1700
EcoRII CCWGG 4 cut(s) 113, 208, 541, 616
EcoT14I CCWWGG 2 cut(s) 725, 2032
EcoT22I ATGCAT 1 cut(s) 2318
EcoT38I GRGCYC 1 cut(s) 2527
ErhI CCWWGG 2 cut(s) 725, 2032
Esp3I CGTCTC 1 cut(s) 408
FaqI GGGAC 5 cut(s) 131, 641, 1421, 1781, 2051
FauNDI CATATG 2 cut(s) 439, 2077
FblI GTMKAC 1 cut(s) 967
Fnu4HI GCNGC 7 cut(s) 184, 1119, 1395, 1599, 1678, 2423, 2460
FokI GGATG 8 cut(s) 161, 571, 1038, 1541, 1547, 1801, 2144, 2359
FriOI GRGCYC 1 cut(s) 2527
Fsp4HI GCNGC 7 cut(s) 184, 1119, 1395, 1599, 1678, 2423, 2460
GluI GCNGC 7 cut(s) 184, 1119, 1395, 1599, 1678, 2423, 2460
GsuI CTGGAG 1 cut(s) 339
HaeIII GGCC 4 cut(s) 657, 730, 1460, 2362
HapII CCGG 3 cut(s) 971, 1562, 2359
HgaI GACGC 2 cut(s) 390, 1115
HindIII AAGCTT 2 cut(s) 1754, 1819
HpaII CCGG 3 cut(s) 971, 1562, 2359
HphI GGTGA 9 cut(s) 319, 725, 870, 1105, 1661, 1709, 1913, 2082, 2408
Hpy166II GTNNAC 3 cut(s) 161, 968, 1264
Hpy188I TCNGA 9 cut(s) 265, 567, 743, 1021, 1584, 1618, 2008, 2131, 2441
Hpy188III TCNNGA 9 cut(s) 318, 478, 1103, 1113, 1417, 1683, 1932, 1979, 2048
Hpy8I GTNNAC 3 cut(s) 161, 968, 1264
HpyCH4III ACNGT 4 cut(s) 274, 941, 1446, 2331
HpyF10VI GCNNNNNNNGC 4 cut(s) 826, 930, 1115, 1457
HpyF3I CTNAG 8 cut(s) 264, 466, 699, 1169, 1681, 1861, 2260, 2418
Kzo9I GATC 7 cut(s) 10, 128, 282, 738, 1282, 1579, 1954
LmnI GCTCC 1 cut(s) 2522
Lsp1109I GCAGC 6 cut(s) 195, 1130, 1610, 1689, 2434, 2446
LweI GCATC 5 cut(s) 388, 835, 1118, 1779, 2303
MaeIII GTNAC 3 cut(s) 603, 893, 2088
MalI GATC 7 cut(s) 12, 130, 284, 740, 1284, 1581, 1956
MboI GATC 7 cut(s) 10, 128, 282, 738, 1282, 1579, 1954
MflI RGATCY 3 cut(s) 10, 1282, 1954
MhlI GDGCHC 1 cut(s) 2527
MlyI GAGTC 3 cut(s) 1050, 1820, 1922
MmeI TCCRAC 3 cut(s) 980, 2050, 2305
Mph1103I ATGCAT 1 cut(s) 2318
MroXI GAANNNNTTC 2 cut(s) 1177, 2446
MseI TTAA 7 cut(s) 125, 536, 590, 1221, 1497, 1623, 1937
MslI CAYNNNNRTG 1 cut(s) 912
MspCI CTTAAG 2 cut(s) 124, 1496
MspI CCGG 3 cut(s) 971, 1562, 2359
MspR9I CCNGG 6 cut(s) 115, 210, 543, 618, 972, 1562
Mva1269I GAATGC 3 cut(s) 1169, 2316, 2488
MvaI CCWGG 4 cut(s) 115, 210, 543, 618
MwoI GCNNNNNNNGC 4 cut(s) 826, 930, 1115, 1457
NciI CCSGG 2 cut(s) 972, 1562
NcoI CCATGG 1 cut(s) 2032
NdeI CATATG 2 cut(s) 439, 2077
NdeII GATC 7 cut(s) 10, 128, 282, 738, 1282, 1579, 1954
NlaIV GGNNCC 3 cut(s) 656, 916, 2039
NmuCI GTSAC 3 cut(s) 603, 893, 2088
NsiI ATGCAT 1 cut(s) 2318
NspI RCATGY 3 cut(s) 1429, 2231, 2240
PaeI GCATGC 1 cut(s) 1429
PaeR7I CTCGAG 1 cut(s) 1930
PciI ACATGT 1 cut(s) 2227
PctI GAATGC 3 cut(s) 1169, 2316, 2488
PdmI GAANNNNTTC 2 cut(s) 1177, 2446
PfeI GAWTC 7 cut(s) 495, 562, 1410, 1744, 2115, 2273, 2348
PkrI GCNGC 7 cut(s) 185, 1120, 1396, 1600, 1679, 2424, 2461
PleI GAGTC 3 cut(s) 1049, 1820, 1922
PpsI GAGTC 3 cut(s) 1049, 1820, 1922
PpuMI RGGWCCY 1 cut(s) 1854
PscI ACATGT 1 cut(s) 2227
PshAI GACNNNNGTC 1 cut(s) 1517
PshBI ATTAAT 1 cut(s) 1221
PsiI TTATAA 3 cut(s) 366, 687, 777
Psp124BI GAGCTC 1 cut(s) 2527
Psp5II RGGWCCY 1 cut(s) 1854
Psp6I CCWGG 4 cut(s) 113, 208, 541, 616
PspGI CCWGG 4 cut(s) 113, 208, 541, 616
PspN4I GGNNCC 3 cut(s) 656, 916, 2039
PspPI GGNCC 5 cut(s) 655, 1459, 1854, 2038, 2356
PspPPI RGGWCCY 1 cut(s) 1854
PstI CTGCAG 1 cut(s) 397
PsuI RGATCY 3 cut(s) 10, 1282, 1954
RsaI GTAC 3 cut(s) 444, 1318, 1467
RsaNI GTAC 3 cut(s) 443, 1317, 1466
RseI CAYNNNNRTG 1 cut(s) 912
SacI GAGCTC 1 cut(s) 2527
SaqAI TTAA 7 cut(s) 125, 536, 590, 1221, 1497, 1623, 1937
SatI GCNGC 7 cut(s) 184, 1119, 1395, 1599, 1678, 2423, 2460
Sau3AI GATC 7 cut(s) 10, 128, 282, 738, 1282, 1579, 1954
Sau96I GGNCC 5 cut(s) 655, 1459, 1854, 2038, 2356
ScaI AGTACT 1 cut(s) 1467
SchI GAGTC 3 cut(s) 1050, 1820, 1922
ScrFI CCNGG 6 cut(s) 115, 210, 543, 618, 972, 1562
SduI GDGCHC 1 cut(s) 2527
SfaNI GCATC 5 cut(s) 388, 835, 1118, 1779, 2303
SfcI CTRYAG 2 cut(s) 393, 525
Sfr274I CTCGAG 1 cut(s) 1930
SinI GGWCC 3 cut(s) 1854, 2038, 2356
SlaI CTCGAG 1 cut(s) 1930
SmiMI CAYNNNNRTG 1 cut(s) 912
SmlI CTYRAG 5 cut(s) 124, 1496, 1541, 1930, 2470
SmoI CTYRAG 5 cut(s) 124, 1496, 1541, 1930, 2470
SpeI ACTAGT 1 cut(s) 1591
SphI GCATGC 1 cut(s) 1429
SsiI CCGC 2 cut(s) 226, 1395
SspI AATATT 2 cut(s) 1972, 2028
SstI GAGCTC 1 cut(s) 2527
StyD4I CCNGG 6 cut(s) 113, 208, 541, 616, 970, 1560
StyI CCWWGG 2 cut(s) 725, 2032
TaaI ACNGT 4 cut(s) 274, 941, 1446, 2331
TaqI TCGA 7 cut(s) 131, 737, 954, 1112, 1578, 1931, 2527
TatI WGTACW 2 cut(s) 442, 1465
TauI GCSGC 1 cut(s) 1397
TfiI GAWTC 7 cut(s) 495, 562, 1410, 1744, 2115, 2273, 2348
Tru1I TTAA 7 cut(s) 125, 536, 590, 1221, 1497, 1623, 1937
Tru9I TTAA 7 cut(s) 125, 536, 590, 1221, 1497, 1623, 1937
TscAI CASTG 3 cut(s) 279, 946, 2109
TseFI GTSAC 3 cut(s) 603, 893, 2088
TseI GCWGC 6 cut(s) 183, 1118, 1598, 1677, 2422, 2459
Tsp45I GTSAC 3 cut(s) 603, 893, 2088
TspGWI ACGGA 1 cut(s) 1290
TspRI CASTG 3 cut(s) 279, 946, 2109
Vha464I CTTAAG 2 cut(s) 124, 1496
VpaK11BI GGWCC 3 cut(s) 1854, 2038, 2356
VspI ATTAAT 1 cut(s) 1221
XapI RAATTY 5 cut(s) 214, 756, 1700, 2001, 2446
XbaI TCTAGA 1 cut(s) 1978
XceI RCATGY 3 cut(s) 1429, 2231, 2240
XhoI CTCGAG 1 cut(s) 1930
XmiI GTMKAC 1 cut(s) 967
XmnI GAANNNNTTC 2 cut(s) 1177, 2446
ZrmI AGTACT 1 cut(s) 1467
Zsp2I ATGCAT 1 cut(s) 2318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.