FvH4_3g06140

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000112
Physical Location & Seq
Reverse (-)
3530694 .. 3534344
3651 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g06140.t1

Sequence Viewer

Length: 2439 bp
ATGGCCCTGTTTCGCACCTACGTAGTTGCAATGTTTCGCAAAGCTCTGTTTCTCTTCCTCCTCTGTCAGTTCTGCACTTCCAGGGACACCATAAGAGGGAACGAAGAGCAACTGAAGGATGTTGATGGAGACTACTTAGTGTCCAAGGAAAGCAAGTTTGAATTGGGGTTCTTCAGCCCCGGCAATTCTAGCTACCGGTATGTTGGAATTTGGTATTCTAAGACTATAGTATCTGAAAGAACAGTGGTGTGGGTTGCAAATAGGAACAATCCCATCAATGATACCTCTGGTGTGCTCAAAATAAACAAGTACGGAGAACTGGCCCTTTATGCTTACAGTGTGGAAAATGATCCTATATGGTTCGCCAATGTGTCACAGTCGGTCCAAAATGTTAACACTAGCACTTTATCTGCACAGCTTTCGGATTCAGGAAACTTAGTTGTGTTCCAGGATGATACAAATGATATCTATATGTGGCAAAGCTTTGATCATCCTACAGATACTCTAATTCCAGGTATGAAACTTGGGGTGAATTGGAAAACTGGGCAAGAATGGGTTTTAACATCTTGGAAGTCACAAGATGACCCTGGAACTGGGGACTGTACCTTTAGGCTCTATCCAAACCAGATTGGATTCCCCCAATTTTTTATGTATAAGGGTTTGAGTAAGTATTGGCGAGTTGATCCTGGGCCAACGCCTCTTGTGGTCAGTAACCAAGAGGAAACTTACGCTTCCTTGAATGCCGATGCAATTACAAGAGCAATAGTTACTGATTCTGTGGAGAAGCGCTTTACTTGGGATGATGACAAACTTCAATGGCATGAAGACTACTCTGCACCAAAGTCCCGGTGTGACTTCTACGGACGGTGTGGTGCCAACAGCAAATGTAGCCCCGACAATGTTAATCTGTTTGAGTGTGAATGCTTACCAGGGTGTGAGCCTAAGTCTATTAGTGATTGGAATCAGAAAAATGGGTCAGGTGGATGTGTTAGTAATCGTGTTGGTTTGTTCAAGTGTGGAGATGGAGATGGGTTTGTGAAGGTGGAAAGAGTTAAATATCCAGACACATCAATAGCAGCATTGTCAAAATCAGGCATGAGTGCCAAAGAGTGTCAGCATGAGTGCCTAGGAAATTGTACTTGCACTGCATATTTGAGCATTAAAAATGAAGGGCTTGATGGCTGCTTCACATGGTATGATGACCTAATGGACATTTTAGGGTACACAGAGCTTGGACCAGATCTCTACGTTCGTGTGAATGCAACTGTGTTAGCTGCATATGTAGGAAAATCACAAGGTTTCTTGGAAAGAAAGGGTATGCTGGCTATTCCAATACTGTCTGCTGTGCTAGCATTGGTACTAACCATCATGCTCGGCTGTTGGTGGCGAAAGAAGAACCACAACACAAAAGCCATTCTGCAGGGAGAGGCGCTTGATGAAACACAGAGACACCCCGACTTACAATTTTTCGATCTTGACACAATAATAGCAGCCACAGACCACTTTTCTCGTGTCAATGAACTTGGCCATGGTGGTTTTGGCTCTGTTTATAAGGGTAAGCTACCAAATGAACAGAATGTTGCTGTGAAAAGGTTGTCCAAAACTTCAGGACAAGGGACTGAAGAATTCAAAAATGAAGTTGCACTTATAGCAAGACTTCAACACAGGAACCTCGTGAAACTTTTAGGCTGCTGTATAAAGGGAGAAGAAAGAATTTTAGTCCTCGAATATATGCCTAATAAAAGCTTGGACTCCTTTCTTTCTGATCACACAAGACGTTCCTTCTTGGACTGGGCAAGGCGCTTTGAGATCATCAATGGGATTGCTCGTGGAATTCTCTATCTTCACCAAGATTCAAGATTGAGAATCATCCATAGAGATCTGAAACCTAGCAATGTTCTACTAGATGCAGAGATGAACCCAAAAATTTCTGATTTTGGCATGGCTAGAATAATCCATGGGAACCAACTTCAAGATAAAACGAGCCGAATTGCTGGAACATATGGCTACATGTCACCAGAATACGCAGTATTTGGGAGATTTTCCACGAAATCTGACATCTTTAGTTTTGGGATCATAATGTTGGAGATTGTAAGTGGCCAGAAAAACAATGGTTCTGATCTAGAGGATCCTTCCATGAATTTCATAGGACATGTTTGGGAGCTCTGGAAGGAAGGTAGAGCCTTAGATATTGTGGATTCGACATTGAAGTCATATCACTCTAATGAAGTCATTAGATGCATACAAGTTGCTCTCTTGTGTGTACAAGAAGATGCAAAGGACCGACCTTCCATGTCAGCTGTTGTTTTCATGTTGAGTGGTGAAGCATCTCCTCCATCTCCTAATCAGCCGGCATTTGTCTACAGAAAATACTCCGGTGCTGATGATGATCCATTACTTTCAAACGACATGACAGTAACTACAATGGAAGGTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

813

Amino Acids

91.3

Weight (kDa)

5.67

Isoelectric Point (pI)

32.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 81 - 191 1.6e-34 D-mannose binding lectin
S_locus_glycop PF00954 242 - 316 1.6e-12 S-locus glycoprotein domain
PAN_2 PF08276 339 - 405 1.2e-17 PAN-like domain
Pkinase PF00069 503 - 768 1.3e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 505 - 769 3.6e-50 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1551
AasI GACNNNNNNGTC 1 cut(s) 2209
AccB1I GGYRCC 1 cut(s) 872
AccI GTMKAC 1 cut(s) 2361
AclWI GGATC 6 cut(s) 344, 677, 2081, 2123, 2136, 2384
AcoI YGGCCR 2 cut(s) 1525, 2098
AcsI RAATTY 6 cut(s) 207, 1625, 1713, 1833, 1926, 2140
AcuI CTGAAG 4 cut(s) 134, 157, 1590, 1641
AfaI GTAC 6 cut(s) 311, 604, 1138, 1223, 1359, 2265
AfeI AGCGCT 1 cut(s) 788
AfiI CCNNNNNNNGG 2 cut(s) 96, 593
AflIII ACRYGT 2 cut(s) 2010, 2152
AgeI ACCGGT 1 cut(s) 195
AjnI CCWGG 6 cut(s) 80, 447, 511, 586, 685, 928
Alw21I GWGCWC 2 cut(s) 297, 2166
Alw26I GTCTC 2 cut(s) 123, 1441
AlwI GGATC 6 cut(s) 344, 677, 2081, 2123, 2136, 2384
Aor51HI AGCGCT 1 cut(s) 788
AoxI GGCC 5 cut(s) 3, 321, 689, 1525, 2098
ApeKI GCWGC 5 cut(s) 1076, 1182, 1274, 1490, 1689
ApoI RAATTY 6 cut(s) 207, 1625, 1713, 1833, 1926, 2140
AsiGI ACCGGT 1 cut(s) 195
AspA2I CCTAGG 1 cut(s) 1126
AspLEI GCGC 3 cut(s) 789, 1432, 1803
AspS9I GGNCC 6 cut(s) 4, 322, 382, 689, 1235, 2281
AsuC2I CCSGG 2 cut(s) 180, 847
AsuHPI GGTGA 4 cut(s) 541, 1838, 2007, 2333
AsuNHI GCTAGC 1 cut(s) 1348
AvaII GGWCC 3 cut(s) 382, 1235, 2281
AvrII CCTAGG 1 cut(s) 1126
BaeI ACNNNNGTAYC 2 cut(s) 213, 246
BalI TGGCCA 2 cut(s) 1527, 2100
BamHI GGATCC 1 cut(s) 2128
BanI GGYRCC 1 cut(s) 872
BanII GRGCYC 1 cut(s) 2166
BauI CACGAG 3 cut(s) 1509, 1673, 1827
BbsI GAAGAC 1 cut(s) 831
Bbv12I GWGCWC 2 cut(s) 297, 2166
BbvI GCAGC 5 cut(s) 1088, 1169, 1261, 1502, 1676
BccI CCATC 7 cut(s) 119, 281, 1016, 1022, 1172, 1373, 2344
BcgI CGANNNNNNTGC 2 cut(s) 402, 436
BciT130I CCWGG 6 cut(s) 82, 449, 513, 588, 687, 930
BclI TGATCA 2 cut(s) 487, 1765
BcnI CCSGG 2 cut(s) 180, 847
BcoDI GTCTC 2 cut(s) 123, 1441
BfaI CTAG 8 cut(s) 189, 399, 1127, 1349, 1890, 1904, 1947, 2123
BfmI CTRYAG 4 cut(s) 225, 495, 1418, 2362
BfoI RGCGCY 3 cut(s) 790, 1433, 1804
BglII AGATCT 2 cut(s) 1240, 1879
BisI GCNGC 5 cut(s) 1077, 1183, 1275, 1491, 1690
BlnI CCTAGG 1 cut(s) 1126
BlsI GCNGC 5 cut(s) 1078, 1184, 1276, 1492, 1691
Bme1390I CCNGG 8 cut(s) 82, 180, 449, 513, 588, 687, 847, 930
Bme18I GGWCC 3 cut(s) 382, 1235, 2281
BmgT120I GGNCC 6 cut(s) 4, 322, 382, 689, 1235, 2281
BmiI GGNNCC 4 cut(s) 874, 1670, 1964, 2130
BmrFI CCNGG 8 cut(s) 82, 180, 449, 513, 588, 687, 847, 930
BmrI ACTGGG 3 cut(s) 552, 603, 1801
BmsI GCATC 5 cut(s) 736, 1897, 2228, 2263, 2336
BmtI GCTAGC 1 cut(s) 1352
BmuI ACTGGG 3 cut(s) 552, 603, 1801
BpiI GAAGAC 1 cut(s) 831
BpuMI CCSGG 2 cut(s) 180, 847
BsaAI YACGTR 1 cut(s) 22
BsaBI GATNNNNATC 2 cut(s) 960, 2388
BsaJI CCNNGG 9 cut(s) 81, 144, 178, 586, 686, 929, 1126, 1528, 1957
BsaWI WCCGGW 2 cut(s) 195, 2375
Bsc4I CCNNNNNNNGG 2 cut(s) 96, 593
Bse118I RCCGGY 2 cut(s) 195, 2350
Bse1I ACTGG 4 cut(s) 324, 547, 598, 1796
Bse3DI GCAATG 2 cut(s) 36, 1900
Bse8I GATNNNNATC 2 cut(s) 960, 2388
BseBI CCWGG 6 cut(s) 82, 449, 513, 588, 687, 930
BseDI CCNNGG 9 cut(s) 81, 144, 178, 586, 686, 929, 1126, 1528, 1957
BseGI GGATG 6 cut(s) 124, 457, 490, 805, 989, 1869
BseJI GATNNNNATC 2 cut(s) 960, 2388
BseLI CCNNNNNNNGG 2 cut(s) 96, 593
BseMI GCAATG 2 cut(s) 36, 1900
BseNI ACTGG 4 cut(s) 324, 547, 598, 1796
BseRI GAGGAG 2 cut(s) 50, 2322
BseXI GCAGC 5 cut(s) 1088, 1169, 1261, 1502, 1676
BsgI GTGCAG 3 cut(s) 58, 396, 819
BshFI GGCC 5 cut(s) 5, 323, 691, 1527, 2100
BshNI GGYRCC 1 cut(s) 872
BshTI ACCGGT 1 cut(s) 195
BsiHKAI GWGCWC 2 cut(s) 297, 2166
BsiSI CCGG 5 cut(s) 180, 196, 847, 2351, 2376
BslFI GGGAC 4 cut(s) 98, 611, 829, 1630
BslI CCNNNNNNNGG 2 cut(s) 96, 593
BsmAI GTCTC 2 cut(s) 123, 1441
BsmFI GGGAC 4 cut(s) 98, 611, 829, 1630
BsmI GAATGC 3 cut(s) 745, 926, 1264
BsnI GGCC 5 cut(s) 5, 323, 691, 1527, 2100
Bsp1286I GDGCHC 2 cut(s) 297, 2166
Bsp1407I TGTACA 1 cut(s) 2263
Bsp19I CCATGG 2 cut(s) 1528, 1957
BspANI GGCC 5 cut(s) 5, 323, 691, 1527, 2100
BspLI GGNNCC 4 cut(s) 874, 1670, 1964, 2130
BspMAI CTGCAG 1 cut(s) 1422
BspOI GCTAGC 1 cut(s) 1352
BspPI GGATC 6 cut(s) 344, 677, 2081, 2123, 2136, 2384
BspQI GCTCTTC 1 cut(s) 99
BspT107I GGYRCC 1 cut(s) 872
BsrDI GCAATG 2 cut(s) 36, 1900
BsrFI RCCGGY 2 cut(s) 195, 2350
BsrGI TGTACA 1 cut(s) 2263
BsrI ACTGG 4 cut(s) 324, 547, 598, 1796
BssAI RCCGGY 2 cut(s) 195, 2350
BssECI CCNNGG 9 cut(s) 81, 144, 178, 586, 686, 929, 1126, 1528, 1957
BssSI CACGAG 3 cut(s) 1509, 1673, 1827
BssT1I CCWWGG 4 cut(s) 144, 1126, 1528, 1957
Bst2BI CACGAG 3 cut(s) 1509, 1673, 1827
Bst2UI CCWGG 6 cut(s) 82, 449, 513, 588, 687, 930
Bst4CI ACNGT 8 cut(s) 244, 338, 378, 602, 867, 1267, 1338, 2416
Bst6I CTCTTC 2 cut(s) 59, 99
BstAUI TGTACA 1 cut(s) 2263
BstBAI YACGTR 1 cut(s) 22
BstC8I GCNNGC 3 cut(s) 1323, 1350, 2352
BstDEI CTNAG 5 cut(s) 136, 219, 436, 942, 2185
BstDSI CCRYGG 2 cut(s) 1528, 1957
BstF5I GGATG 6 cut(s) 124, 457, 490, 805, 989, 1869
BstH2I RGCGCY 3 cut(s) 790, 1433, 1804
BstHHI GCGC 3 cut(s) 789, 1432, 1803
BstMAI GTCTC 2 cut(s) 123, 1441
BstMWI GCNNNNNNNGC 5 cut(s) 189, 329, 888, 1349, 1649
BstNI CCWGG 6 cut(s) 82, 449, 513, 588, 687, 930
BstNSI RCATGY 2 cut(s) 2014, 2156
BstSCI CCNGG 8 cut(s) 80, 178, 447, 511, 586, 685, 845, 928
BstSFI CTRYAG 4 cut(s) 225, 495, 1418, 2362
BstSNI TACGTA 1 cut(s) 22
BstV1I GCAGC 5 cut(s) 1088, 1169, 1261, 1502, 1676
BstV2I GAAGAC 1 cut(s) 831
BstX2I RGATCY 3 cut(s) 1240, 1879, 2128
BstYI RGATCY 3 cut(s) 1240, 1879, 2128
BsuRI GGCC 5 cut(s) 5, 323, 691, 1527, 2100
BtgI CCRYGG 2 cut(s) 1528, 1957
BtsCI GGATG 6 cut(s) 124, 457, 490, 805, 989, 1869
BtsI GCAGTG 1 cut(s) 1143
BtsIMutI CAGTG 3 cut(s) 249, 343, 1143
Cac8I GCNNGC 3 cut(s) 1323, 1350, 2352
CfoI GCGC 3 cut(s) 789, 1432, 1803
Cfr10I RCCGGY 2 cut(s) 195, 2350
Cfr13I GGNCC 6 cut(s) 4, 322, 382, 689, 1235, 2281
Csp6I GTAC 6 cut(s) 310, 603, 1137, 1222, 1358, 2264
CspAI ACCGGT 1 cut(s) 195
CviQI GTAC 6 cut(s) 310, 603, 1137, 1222, 1358, 2264
DdeI CTNAG 5 cut(s) 136, 219, 436, 942, 2185
DrdI GACNNNNNNGTC 1 cut(s) 2209
DseDI GACNNNNNNGTC 1 cut(s) 2209
EaeI YGGCCR 2 cut(s) 1525, 2098
Eam1104I CTCTTC 2 cut(s) 59, 99
EarI CTCTTC 2 cut(s) 59, 99
Ecl136II GAGCTC 1 cut(s) 2164
Eco105I TACGTA 1 cut(s) 22
Eco130I CCWWGG 4 cut(s) 144, 1126, 1528, 1957
Eco24I GRGCYC 1 cut(s) 2166
Eco32I GATATC 1 cut(s) 466
Eco47I GGWCC 3 cut(s) 382, 1235, 2281
Eco47III AGCGCT 1 cut(s) 788
Eco53kI GAGCTC 1 cut(s) 2164
Eco57I CTGAAG 4 cut(s) 134, 157, 1590, 1641
EcoICRI GAGCTC 1 cut(s) 2164
EcoRI GAATTC 2 cut(s) 1625, 1833
EcoRII CCWGG 6 cut(s) 80, 447, 511, 586, 685, 928
EcoRV GATATC 1 cut(s) 466
EcoT14I CCWWGG 4 cut(s) 144, 1126, 1528, 1957
EcoT22I ATGCAT 1 cut(s) 2243
EcoT38I GRGCYC 1 cut(s) 2166
ErhI CCWWGG 4 cut(s) 144, 1126, 1528, 1957
FalI AAGNNNNNCTT 2 cut(s) 1629, 1661
FaqI GGGAC 4 cut(s) 98, 611, 829, 1630
FauNDI CATATG 2 cut(s) 1279, 2002
FbaI TGATCA 2 cut(s) 487, 1765
FblI GTMKAC 1 cut(s) 2361
Fnu4HI GCNGC 5 cut(s) 1077, 1183, 1275, 1491, 1690
FokI GGATG 6 cut(s) 131, 464, 477, 812, 996, 1856
FriOI GRGCYC 1 cut(s) 2166
Fsp4HI GCNGC 5 cut(s) 1077, 1183, 1275, 1491, 1690
FspBI CTAG 8 cut(s) 189, 399, 1127, 1349, 1890, 1904, 1947, 2123
GlaI GCGC 3 cut(s) 788, 1431, 1802
GluI GCNGC 5 cut(s) 1077, 1183, 1275, 1491, 1690
HaeII RGCGCY 3 cut(s) 790, 1433, 1804
HaeIII GGCC 5 cut(s) 5, 323, 691, 1527, 2100
HapII CCGG 5 cut(s) 180, 196, 847, 2351, 2376
HhaI GCGC 3 cut(s) 789, 1432, 1803
Hin6I GCGC 3 cut(s) 787, 1430, 1801
HinP1I GCGC 3 cut(s) 787, 1430, 1801
HincII GTYRAC 1 cut(s) 394
HindII GTYRAC 1 cut(s) 394
HindIII AAGCTT 2 cut(s) 481, 1744
HinfI GANTC 8 cut(s) 425, 633, 773, 961, 1751, 1853, 1866, 2198
HpaI GTTAAC 1 cut(s) 394
HpaII CCGG 5 cut(s) 180, 196, 847, 2351, 2376
HphI GGTGA 4 cut(s) 541, 1838, 2007, 2333
Hpy166II GTNNAC 4 cut(s) 394, 1224, 2264, 2362
Hpy188I TCNGA 8 cut(s) 235, 424, 966, 1765, 1884, 1933, 2056, 2119
Hpy188III TCNNGA 9 cut(s) 429, 1061, 1475, 1608, 1675, 1857, 1973, 2123, 2167
Hpy8I GTNNAC 4 cut(s) 394, 1224, 2264, 2362
HpyAV CCTTC 9 cut(s) 109, 1033, 1163, 1792, 2142, 2164, 2168, 2298, 2423
HpyCH4III ACNGT 8 cut(s) 244, 338, 378, 602, 867, 1267, 1338, 2416
HpyCH4IV ACGT 3 cut(s) 21, 1248, 1777
HpyF10VI GCNNNNNNNGC 5 cut(s) 189, 329, 888, 1349, 1649
HpyF3I CTNAG 5 cut(s) 136, 219, 436, 942, 2185
HpySE526I ACGT 3 cut(s) 21, 1248, 1777
HspAI GCGC 3 cut(s) 787, 1430, 1801
KroI GCCGGC 1 cut(s) 2350
KroNI GCCGGC 1 cut(s) 2352
Ksp22I TGATCA 2 cut(s) 487, 1765
KspAI GTTAAC 1 cut(s) 394
LguI GCTCTTC 1 cut(s) 99
LmnI GCTCC 1 cut(s) 2161
Lsp1109I GCAGC 5 cut(s) 1088, 1169, 1261, 1502, 1676
LweI GCATC 5 cut(s) 736, 1897, 2228, 2263, 2336
MaeI CTAG 8 cut(s) 189, 399, 1127, 1349, 1890, 1904, 1947, 2123
MaeII ACGT 3 cut(s) 21, 1248, 1777
MaeIII GTNAC 7 cut(s) 372, 573, 710, 766, 851, 2013, 2416
MboII GAAGA 9 cut(s) 46, 116, 163, 836, 1405, 1634, 1718, 1835, 2282
MflI RGATCY 3 cut(s) 1240, 1879, 2128
MhlI GDGCHC 2 cut(s) 297, 2166
MlsI TGGCCA 2 cut(s) 1527, 2100
MluNI TGGCCA 2 cut(s) 1527, 2100
MlyI GAGTC 1 cut(s) 1745
MmeI TCCRAC 2 cut(s) 184, 2064
Mox20I TGGCCA 2 cut(s) 1527, 2100
Mph1103I ATGCAT 1 cut(s) 2243
MroNI GCCGGC 1 cut(s) 2350
MscI TGGCCA 2 cut(s) 1527, 2100
MseI TTAA 5 cut(s) 393, 560, 903, 1053, 1161
MslI CAYNNNNRTG 1 cut(s) 2223
Msp20I TGGCCA 2 cut(s) 1527, 2100
MspA1I CMGCKG 1 cut(s) 2300
MspI CCGG 5 cut(s) 180, 196, 847, 2351, 2376
MspR9I CCNGG 8 cut(s) 82, 180, 449, 513, 588, 687, 847, 930
Mva1269I GAATGC 3 cut(s) 745, 926, 1264
MvaI CCWGG 6 cut(s) 82, 449, 513, 588, 687, 930
MwoI GCNNNNNNNGC 5 cut(s) 189, 329, 888, 1349, 1649
NaeI GCCGGC 1 cut(s) 2352
NciI CCSGG 2 cut(s) 180, 847
NcoI CCATGG 2 cut(s) 1528, 1957
NdeI CATATG 2 cut(s) 1279, 2002
NgoMIV GCCGGC 1 cut(s) 2350
NheI GCTAGC 1 cut(s) 1348
NlaIV GGNNCC 4 cut(s) 874, 1670, 1964, 2130
NmeAIII GCCGAG 1 cut(s) 1353
NmuCI GTSAC 4 cut(s) 372, 573, 851, 2013
NsiI ATGCAT 1 cut(s) 2243
NspI RCATGY 2 cut(s) 2014, 2156
PciI ACATGT 2 cut(s) 2010, 2152
PciSI GCTCTTC 1 cut(s) 99
PctI GAATGC 3 cut(s) 745, 926, 1264
PdiI GCCGGC 1 cut(s) 2352
PfeI GAWTC 7 cut(s) 425, 633, 773, 961, 1853, 1866, 2198
PfoI TCCNGGA 1 cut(s) 447
PinAI ACCGGT 1 cut(s) 195
PkrI GCNGC 5 cut(s) 1078, 1184, 1276, 1492, 1691
PleI GAGTC 1 cut(s) 1745
PpsI GAGTC 1 cut(s) 1745
Ppu21I YACGTR 1 cut(s) 22
PscI ACATGT 2 cut(s) 2010, 2152
PsiI TTATAA 1 cut(s) 1551
Psp124BI GAGCTC 1 cut(s) 2166
Psp6I CCWGG 6 cut(s) 80, 447, 511, 586, 685, 928
PspGI CCWGG 6 cut(s) 80, 447, 511, 586, 685, 928
PspN4I GGNNCC 4 cut(s) 874, 1670, 1964, 2130
PspPI GGNCC 6 cut(s) 4, 322, 382, 689, 1235, 2281
PstI CTGCAG 1 cut(s) 1422
PsuI RGATCY 3 cut(s) 1240, 1879, 2128
PvuII CAGCTG 1 cut(s) 2300
RsaI GTAC 6 cut(s) 311, 604, 1138, 1223, 1359, 2265
RsaNI GTAC 6 cut(s) 310, 603, 1137, 1222, 1358, 2264
RseI CAYNNNNRTG 1 cut(s) 2223
SacI GAGCTC 1 cut(s) 2166
SapI GCTCTTC 1 cut(s) 99
SaqAI TTAA 5 cut(s) 393, 560, 903, 1053, 1161
SatI GCNGC 5 cut(s) 1077, 1183, 1275, 1491, 1690
Sau96I GGNCC 6 cut(s) 4, 322, 382, 689, 1235, 2281
SchI GAGTC 1 cut(s) 1745
ScrFI CCNGG 8 cut(s) 82, 180, 449, 513, 588, 687, 847, 930
SduI GDGCHC 2 cut(s) 297, 2166
SfaNI GCATC 5 cut(s) 736, 1897, 2228, 2263, 2336
SfcI CTRYAG 4 cut(s) 225, 495, 1418, 2362
SinI GGWCC 3 cut(s) 382, 1235, 2281
SmiMI CAYNNNNRTG 1 cut(s) 2223
SnaBI TACGTA 1 cut(s) 22
SspMI CTAG 8 cut(s) 189, 399, 1127, 1349, 1890, 1904, 1947, 2123
SstI GAGCTC 1 cut(s) 2166
StyD4I CCNGG 8 cut(s) 80, 178, 447, 511, 586, 685, 845, 928
StyI CCWWGG 4 cut(s) 144, 1126, 1528, 1957
TaaI ACNGT 8 cut(s) 244, 338, 378, 602, 867, 1267, 1338, 2416
TaiI ACGT 3 cut(s) 24, 1251, 1780
TaqI TCGA 4 cut(s) 1470, 1725, 2201, 2434
TaqII GACCGA 2 cut(s) 370, 2298
TatI WGTACW 2 cut(s) 1136, 2263
TfiI GAWTC 7 cut(s) 425, 633, 773, 961, 1853, 1866, 2198
Tru1I TTAA 5 cut(s) 393, 560, 903, 1053, 1161
Tru9I TTAA 5 cut(s) 393, 560, 903, 1053, 1161
TscAI CASTG 3 cut(s) 249, 343, 1150
TseFI GTSAC 4 cut(s) 372, 573, 851, 2013
TseI GCWGC 5 cut(s) 1076, 1182, 1274, 1490, 1689
Tsp45I GTSAC 4 cut(s) 372, 573, 851, 2013
TspGWI ACGGA 2 cut(s) 327, 876
TspRI CASTG 3 cut(s) 249, 343, 1150
VpaK11BI GGWCC 3 cut(s) 382, 1235, 2281
XapI RAATTY 6 cut(s) 207, 1625, 1713, 1833, 1926, 2140
XbaI TCTAGA 1 cut(s) 2122
XceI RCATGY 2 cut(s) 2014, 2156
XcmI CCANNNNNNNNNTGG 2 cut(s) 1535, 2108
XmaJI CCTAGG 1 cut(s) 1126
XmiI GTMKAC 1 cut(s) 2361
XspI CTAG 8 cut(s) 189, 399, 1127, 1349, 1890, 1904, 1947, 2123
Zsp2I ATGCAT 1 cut(s) 2243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.