MD05G1328500.v1.1

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000112
Physical Location & Seq
Forward (+)
45385089 .. 45387203
2115 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1328500.v1.1.491

Sequence Viewer

Length: 1212 bp
ATGGTCATAGAATCTGAAGGGCCCAGTGATTGCTTGACATGGTATGGTGAGTTGCTGGACATTTTAGTGCACACACAGGTAGGGCGAGATCTACACGTCCGTGTGGACAAAATCGAATTAGCTGAAACTGCCCGAAACTCGAAAGGTTTTCTTAAAAGGAGAGTTGAGATCGAAGAAACTAGGGGACATCCCGAATTGCAATTTTTCCATCTGAGAACGATAATTGCAGCTACAAACAACTTCTCTCCAGTCCAAAAACTTGGCCAAGGTGGTTTTGGCACTGTTTATAAGGGTGTGTTACGAAATAATCAGAATATTGCTGTAAAAAGATTATCCAGAACTTCAGGACAAGGAGTTGAAGAATTCAAAAATGAAGTTGCGCTGATAGCGAGACTTCAACACAGGAATCTTGTGAAACTTTTAGGTTGTTGCATAAAGGGAGAAGAAAGGATGTTAGTCCTGGAATACTTGCCGAACAAAAGCTTGGACTATTTTCTTTTCGATCACACAAAAAAGTCCTTGCTGGATTGGCAAAAGCTGGATTGGCAAAAGCGATTCGAAATCATCAATGGGGTTGCTCGTGGGGTTTTGTATCTTCACCAGGACTCAAGACTTAGGATTATTCACAGGGATCTAAAAACCAGCAATGTCCTTCTAGACGCTGAGATGAACCCAAAAATTTCAGATTTCGGCATGGCAAGAATCTTCTATGGGGACCAACTCCAGGATACGACCAACAAAGTTGTTGGAACATATGGCTATATGTCACCAGAGTATGTAGTGTTTGGGAGATTTTCAACCAAATCAGATGTTTTTAGTTTTGGGGTCATATTATTGGAGATTGTAAGCGGCAAGAAAAACAACAGTTTCGATCAAGAGGATCATTTCAGAAACTTAATAGGACATGTTTGGCAGTTGTGGAGTGAAGACAGAGCCTTAGAAATTGTGGATTTGTCACTGGAGTCATACTCACCCGACGAAGCCATGAGATGCATTCAAGTCGGGCTCTTGTGTGTCGAAGAAGAGTCGAAGAACAGACCATCCATGTCAGCTGTTGTTTTTATGTTGAGCGGCGAAGCATCTGCTCCATCTCCTCAGCAGCCTGCATTTGCCTTGAGAAAAAATTCATGCAGCGATGCTGCTGTTCCATCAGTTTCCAAAGGATCGTGTTCTATCAACGACGTGACAATAACTAAATTTGAAGGTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

404

Amino Acids

45.66

Weight (kDa)

6.81

Isoelectric Point (pI)

44.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 83 - 351 5.5e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 85 - 353 9.1e-51 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 288
AccBSI CCGCTC 1 cut(s) 1071
AciI CCGC 2 cut(s) 849, 1071
AclWI GGATC 3 cut(s) 639, 888, 1171
AcoI YGGCCR 1 cut(s) 262
AcsI RAATTY 4 cut(s) 362, 678, 1123, 1196
AcuI CTGAAG 2 cut(s) 36, 327
AfiI CCNNNNNNNGG 1 cut(s) 724
AflIII ACRYGT 2 cut(s) 94, 904
AgsI TTSAA 6 cut(s) 359, 367, 398, 798, 998, 1202
AjiI CACGTC 2 cut(s) 97, 1183
AjnI CCWGG 3 cut(s) 459, 600, 723
AjuI GAANNNNNNNTTGG 2 cut(s) 467, 499
AleI CACNNNNGTG 1 cut(s) 99
AloI GAACNNNNNNTCC 2 cut(s) 1025, 1057
AluBI AGCT 5 cut(s) 122, 230, 483, 538, 1052
AluI AGCT 5 cut(s) 122, 230, 483, 538, 1052
Alw21I GWGCWC 1 cut(s) 72
Alw26I GTCTC 1 cut(s) 385
Alw44I GTGCAC 1 cut(s) 68
AlwI GGATC 3 cut(s) 639, 888, 1171
AoxI GGCC 2 cut(s) 20, 262
ApaI GGGCCC 1 cut(s) 24
ApaLI GTGCAC 1 cut(s) 68
ApeKI GCWGC 4 cut(s) 227, 1099, 1131, 1139
ApoI RAATTY 4 cut(s) 362, 678, 1123, 1196
Asp700I GAANNNNTTC 1 cut(s) 1123
AspLEI GCGC 1 cut(s) 382
AspS9I GGNCC 3 cut(s) 20, 21, 715
AsuHPI GGTGA 4 cut(s) 59, 590, 759, 963
AsuII TTCGAA 1 cut(s) 558
AvaII GGWCC 1 cut(s) 715
BaeGI GKGCMC 2 cut(s) 24, 72
BalI TGGCCA 1 cut(s) 264
BanII GRGCYC 2 cut(s) 24, 1008
BauI CACGAG 1 cut(s) 579
BbsI GAAGAC 1 cut(s) 933
Bbv12I GWGCWC 1 cut(s) 72
BbvCI CCTCAGC 1 cut(s) 1095
BbvI GCAGC 4 cut(s) 239, 1111, 1126, 1143
BccI CCATC 4 cut(s) 216, 1048, 1096, 1156
BcgI CGANNNNNNTGC 4 cut(s) 982, 1016, 1064, 1098
BciT130I CCWGG 3 cut(s) 461, 602, 725
BciVI GTATCC 1 cut(s) 721
BcoDI GTCTC 1 cut(s) 385
BfaI CTAG 2 cut(s) 180, 656
BfuI GTATCC 1 cut(s) 721
BglII AGATCT 1 cut(s) 88
BisI GCNGC 6 cut(s) 228, 850, 1072, 1100, 1132, 1140
BlsI GCNGC 6 cut(s) 229, 851, 1073, 1101, 1133, 1141
Bme1390I CCNGG 3 cut(s) 461, 602, 725
Bme18I GGWCC 1 cut(s) 715
BmgBI CACGTC 2 cut(s) 97, 1183
BmgT120I GGNCC 3 cut(s) 20, 21, 715
BmiI GGNNCC 2 cut(s) 22, 716
BmrFI CCNGG 3 cut(s) 461, 602, 725
BmrI ACTGGG 1 cut(s) 18
BmsI GCATC 3 cut(s) 980, 1088, 1126
BmuI ACTGGG 1 cut(s) 18
BpiI GAAGAC 1 cut(s) 933
BplI GAGNNNNNCTC 2 cut(s) 953, 985
BpmI CTGGAG 3 cut(s) 231, 707, 980
Bpu10I CCTNAGC 1 cut(s) 1095
Bpu14I TTCGAA 1 cut(s) 558
BpuEI CTTGAG 2 cut(s) 592, 1135
BsaJI CCNNGG 1 cut(s) 265
Bsc4I CCNNNNNNNGG 1 cut(s) 724
Bse1I ACTGG 3 cut(s) 24, 248, 963
Bse3DI GCAATG 1 cut(s) 652
BseBI CCWGG 3 cut(s) 461, 602, 725
BseDI CCNNGG 1 cut(s) 265
BseGI GGATG 3 cut(s) 187, 456, 1040
BseLI CCNNNNNNNGG 1 cut(s) 724
BseMI GCAATG 1 cut(s) 652
BseMII CTCAG 3 cut(s) 203, 654, 1109
BseNI ACTGG 3 cut(s) 24, 248, 963
BseRI GAGGAG 1 cut(s) 1083
BseSI GKGCMC 2 cut(s) 24, 72
BseXI GCAGC 4 cut(s) 239, 1111, 1126, 1143
BshFI GGCC 2 cut(s) 22, 264
BsiHKAI GWGCWC 1 cut(s) 72
BslFI GGGAC 2 cut(s) 198, 728
BslI CCNNNNNNNGG 1 cut(s) 724
BsmAI GTCTC 1 cut(s) 385
BsmFI GGGAC 2 cut(s) 198, 728
BsmI GAATGC 1 cut(s) 993
BsnI GGCC 2 cut(s) 22, 264
Bsp119I TTCGAA 1 cut(s) 558
Bsp120I GGGCCC 1 cut(s) 20
Bsp1286I GDGCHC 3 cut(s) 24, 72, 1008
Bsp143I GATC 7 cut(s) 88, 168, 502, 631, 871, 880, 1163
BspACI CCGC 2 cut(s) 849, 1071
BspANI GGCC 2 cut(s) 22, 264
BspCNI CTCAG 3 cut(s) 204, 655, 1108
BspLI GGNNCC 2 cut(s) 22, 716
BspPI GGATC 3 cut(s) 639, 888, 1171
BspT104I TTCGAA 1 cut(s) 558
BsrBI CCGCTC 1 cut(s) 1071
BsrDI GCAATG 1 cut(s) 652
BsrI ACTGG 3 cut(s) 24, 248, 963
BssECI CCNNGG 1 cut(s) 265
BssMI GATC 7 cut(s) 88, 168, 502, 631, 871, 880, 1163
BssSI CACGAG 1 cut(s) 579
BssT1I CCWWGG 1 cut(s) 265
Bst2BI CACGAG 1 cut(s) 579
Bst2UI CCWGG 3 cut(s) 461, 602, 725
Bst4CI ACNGT 2 cut(s) 283, 866
Bst6I CTCTTC 1 cut(s) 1017
BstBI TTCGAA 1 cut(s) 558
BstC8I GCNNGC 1 cut(s) 1104
BstDEI CTNAG 5 cut(s) 212, 614, 663, 937, 1095
BstF5I GGATG 3 cut(s) 187, 456, 1040
BstHHI GCGC 1 cut(s) 382
BstKTI GATC 7 cut(s) 91, 171, 505, 634, 874, 883, 1166
BstMAI GTCTC 1 cut(s) 385
BstMBI GATC 7 cut(s) 88, 168, 502, 631, 871, 880, 1163
BstMWI GCNNNNNNNGC 4 cut(s) 128, 386, 529, 544
BstNI CCWGG 3 cut(s) 461, 602, 725
BstNSI RCATGY 1 cut(s) 908
BstSCI CCNGG 3 cut(s) 459, 600, 723
BstSLI GKGCMC 2 cut(s) 24, 72
BstV1I GCAGC 4 cut(s) 239, 1111, 1126, 1143
BstV2I GAAGAC 1 cut(s) 933
BstX2I RGATCY 2 cut(s) 88, 631
BstXI CCANNNNNNTGG 1 cut(s) 260
BstYI RGATCY 2 cut(s) 88, 631
BsuI GTATCC 1 cut(s) 721
BsuRI GGCC 2 cut(s) 22, 264
BtgZI GCGATG 1 cut(s) 1149
BtrI CACGTC 2 cut(s) 97, 1183
BtsCI GGATG 3 cut(s) 187, 456, 1040
BtsIMutI CAGTG 3 cut(s) 31, 279, 956
Cac8I GCNNGC 1 cut(s) 1104
CfoI GCGC 1 cut(s) 382
Cfr13I GGNCC 3 cut(s) 20, 21, 715
CseI GACGC 1 cut(s) 668
CviAII CATG 6 cut(s) 39, 694, 905, 985, 1045, 1128
DdeI CTNAG 5 cut(s) 212, 614, 663, 937, 1095
DpnI GATC 7 cut(s) 90, 170, 504, 633, 873, 882, 1165
DpnII GATC 7 cut(s) 88, 168, 502, 631, 871, 880, 1163
EaeI YGGCCR 1 cut(s) 262
Eam1104I CTCTTC 1 cut(s) 1017
EarI CTCTTC 1 cut(s) 1017
Eco130I CCWWGG 1 cut(s) 265
Eco24I GRGCYC 2 cut(s) 24, 1008
Eco47I GGWCC 1 cut(s) 715
Eco57I CTGAAG 2 cut(s) 36, 327
EcoO109I RGGNCCY 1 cut(s) 20
EcoRI GAATTC 1 cut(s) 362
EcoRII CCWGG 3 cut(s) 459, 600, 723
EcoT14I CCWWGG 1 cut(s) 265
EcoT22I ATGCAT 1 cut(s) 995
EcoT38I GRGCYC 2 cut(s) 24, 1008
ErhI CCWWGG 1 cut(s) 265
FaeI CATG 6 cut(s) 42, 697, 908, 988, 1048, 1131
FalI AAGNNNNNCTT 2 cut(s) 135, 167
FaqI GGGAC 2 cut(s) 198, 728
FatI CATG 6 cut(s) 38, 693, 904, 984, 1044, 1127
FauNDI CATATG 1 cut(s) 754
Fnu4HI GCNGC 6 cut(s) 228, 850, 1072, 1100, 1132, 1140
FokI GGATG 3 cut(s) 174, 463, 1027
FriOI GRGCYC 2 cut(s) 24, 1008
Fsp4HI GCNGC 6 cut(s) 228, 850, 1072, 1100, 1132, 1140
FspBI CTAG 2 cut(s) 180, 656
GlaI GCGC 1 cut(s) 381
GluI GCNGC 6 cut(s) 228, 850, 1072, 1100, 1132, 1140
GsuI CTGGAG 3 cut(s) 231, 707, 980
HaeIII GGCC 2 cut(s) 22, 264
HgaI GACGC 1 cut(s) 668
HhaI GCGC 1 cut(s) 382
Hin1II CATG 6 cut(s) 42, 697, 908, 988, 1048, 1131
Hin6I GCGC 1 cut(s) 380
HinP1I GCGC 1 cut(s) 380
HindIII AAGCTT 1 cut(s) 481
HinfI GANTC 7 cut(s) 11, 406, 555, 605, 702, 962, 1025
HphI GGTGA 4 cut(s) 59, 590, 759, 963
Hpy166II GTNNAC 2 cut(s) 70, 106
Hpy188I TCNGA 6 cut(s) 16, 213, 312, 685, 808, 890
Hpy188III TCNNGA 6 cut(s) 191, 336, 345, 609, 656, 875
Hpy8I GTNNAC 2 cut(s) 70, 106
Hpy99I CGWCG 2 cut(s) 980, 1184
HpyAV CCTTC 3 cut(s) 11, 662, 1196
HpyCH4III ACNGT 2 cut(s) 283, 866
HpyCH4IV ACGT 2 cut(s) 96, 1182
HpyCH4V TGCA 7 cut(s) 70, 199, 227, 432, 993, 1106, 1131
HpyF10VI GCNNNNNNNGC 4 cut(s) 128, 386, 529, 544
HpyF3I CTNAG 5 cut(s) 212, 614, 663, 937, 1095
HpySE526I ACGT 2 cut(s) 96, 1182
Hsp92II CATG 6 cut(s) 42, 697, 908, 988, 1048, 1131
HspAI GCGC 1 cut(s) 380
Kzo9I GATC 7 cut(s) 88, 168, 502, 631, 871, 880, 1163
LmnI GCTCC 1 cut(s) 1090
Lsp1109I GCAGC 4 cut(s) 239, 1111, 1126, 1143
LweI GCATC 3 cut(s) 980, 1088, 1126
MaeI CTAG 2 cut(s) 180, 656
MaeII ACGT 2 cut(s) 96, 1182
MaeIII GTNAC 4 cut(s) 297, 765, 954, 1183
MalI GATC 7 cut(s) 90, 170, 504, 633, 873, 882, 1165
MbiI CCGCTC 1 cut(s) 1071
MboI GATC 7 cut(s) 88, 168, 502, 631, 871, 880, 1163
MboII GAAGA 9 cut(s) 185, 371, 455, 587, 697, 938, 1031, 1034, 1042
MflI RGATCY 2 cut(s) 88, 631
MhlI GDGCHC 3 cut(s) 24, 72, 1008
MlsI TGGCCA 1 cut(s) 264
MluCI AATT 9 cut(s) 116, 194, 200, 222, 362, 678, 942, 1123, 1196
MluNI TGGCCA 1 cut(s) 264
MlyI GAGTC 3 cut(s) 599, 971, 1034
MmeI TCCRAC 1 cut(s) 727
MnlI CCTC 2 cut(s) 871, 1104
Mox20I TGGCCA 1 cut(s) 264
Mph1103I ATGCAT 1 cut(s) 995
MroXI GAANNNNTTC 1 cut(s) 1123
MscI TGGCCA 1 cut(s) 264
MseI TTAA 2 cut(s) 153, 896
MslI CAYNNNNRTG 2 cut(s) 65, 99
Msp20I TGGCCA 1 cut(s) 264
MspA1I CMGCKG 1 cut(s) 1052
MspR9I CCNGG 3 cut(s) 461, 602, 725
Mva1269I GAATGC 1 cut(s) 993
MvaI CCWGG 3 cut(s) 461, 602, 725
MwoI GCNNNNNNNGC 4 cut(s) 128, 386, 529, 544
NdeI CATATG 1 cut(s) 754
NdeII GATC 7 cut(s) 88, 168, 502, 631, 871, 880, 1163
NlaIII CATG 6 cut(s) 42, 697, 908, 988, 1048, 1131
NlaIV GGNNCC 2 cut(s) 22, 716
NmuCI GTSAC 3 cut(s) 765, 954, 1183
NsiI ATGCAT 1 cut(s) 995
NspI RCATGY 1 cut(s) 908
NspV TTCGAA 1 cut(s) 558
OliI CACNNNNGTG 1 cut(s) 99
PciI ACATGT 1 cut(s) 904
PctI GAATGC 1 cut(s) 993
PdmI GAANNNNTTC 1 cut(s) 1123
PfeI GAWTC 4 cut(s) 11, 406, 555, 702
PfoI TCCNGGA 2 cut(s) 459, 723
PkrI GCNGC 6 cut(s) 229, 851, 1073, 1101, 1133, 1141
PleI GAGTC 3 cut(s) 599, 970, 1033
PpsI GAGTC 3 cut(s) 599, 970, 1033
PscI ACATGT 1 cut(s) 904
PsiI TTATAA 1 cut(s) 288
Psp6I CCWGG 3 cut(s) 459, 600, 723
PspGI CCWGG 3 cut(s) 459, 600, 723
PspN4I GGNNCC 2 cut(s) 22, 716
PspOMI GGGCCC 1 cut(s) 20
PspPI GGNCC 3 cut(s) 20, 21, 715
PsuI RGATCY 2 cut(s) 88, 631
PvuII CAGCTG 1 cut(s) 1052
RseI CAYNNNNRTG 2 cut(s) 65, 99
SaqAI TTAA 2 cut(s) 153, 896
SatI GCNGC 6 cut(s) 228, 850, 1072, 1100, 1132, 1140
Sau3AI GATC 7 cut(s) 88, 168, 502, 631, 871, 880, 1163
Sau96I GGNCC 3 cut(s) 20, 21, 715
SchI GAGTC 3 cut(s) 599, 971, 1034
ScrFI CCNGG 3 cut(s) 461, 602, 725
SduI GDGCHC 3 cut(s) 24, 72, 1008
SfaNI GCATC 3 cut(s) 980, 1088, 1126
SfuI TTCGAA 1 cut(s) 558
SinI GGWCC 1 cut(s) 715
SmiMI CAYNNNNRTG 2 cut(s) 65, 99
SmlI CTYRAG 2 cut(s) 607, 1114
SmoI CTYRAG 2 cut(s) 607, 1114
Sse9I AATT 9 cut(s) 116, 194, 200, 222, 362, 678, 942, 1123, 1196
SsiI CCGC 2 cut(s) 849, 1071
SspI AATATT 1 cut(s) 316
SspMI CTAG 2 cut(s) 180, 656
StyD4I CCNGG 3 cut(s) 459, 600, 723
StyI CCWWGG 1 cut(s) 265
TaaI ACNGT 2 cut(s) 283, 866
TaiI ACGT 2 cut(s) 99, 1185
TaqI TCGA 9 cut(s) 114, 140, 171, 501, 558, 870, 1017, 1028, 1207
TasI AATT 9 cut(s) 116, 194, 200, 222, 362, 678, 942, 1123, 1196
TauI GCSGC 2 cut(s) 852, 1074
TfiI GAWTC 4 cut(s) 11, 406, 555, 702
Tru1I TTAA 2 cut(s) 153, 896
Tru9I TTAA 2 cut(s) 153, 896
TscAI CASTG 3 cut(s) 31, 286, 963
TseFI GTSAC 3 cut(s) 765, 954, 1183
TseI GCWGC 4 cut(s) 227, 1099, 1131, 1139
Tsp45I GTSAC 3 cut(s) 765, 954, 1183
TspDTI ATGAA 3 cut(s) 387, 683, 1116
TspGWI ACGGA 1 cut(s) 89
TspRI CASTG 3 cut(s) 31, 286, 963
VneI GTGCAC 1 cut(s) 68
VpaK11BI GGWCC 1 cut(s) 715
XapI RAATTY 4 cut(s) 362, 678, 1123, 1196
XbaI TCTAGA 1 cut(s) 655
XceI RCATGY 1 cut(s) 908
XcmI CCANNNNNNNNNTGG 1 cut(s) 272
XmnI GAANNNNTTC 1 cut(s) 1123
XspI CTAG 2 cut(s) 180, 656
Zsp2I ATGCAT 1 cut(s) 995
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.