Rw5G013960

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000112
Physical Location & Seq
Reverse (-)
16540014 .. 16543217
3204 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G013960.1

Sequence Viewer

Length: 2469 bp
ATGCCAGGGTTTCATACCTACAAAGTTGCTAATGCCCAGAAAATGTTATTCAAAGCTCTGCTTCTCTTCCTTCTCTTCCGATTTTGCGAATCCAGGGACACCATAACATGGAACCAAGAACAACTGAAGGATGATGGTGGAGTTTTAGTGTCCAAACAGAGCAAGTTCGAATTGGGTTTCTTCAGCCCCGGAAATTCTAGCTACCGGTATGTTGGAATCTGGTATTCTCAGACTAGAGTACCTAATAAAACAGTGGTGTGGGTTGCAAACAGGAACAATCCCATCAATGATACCTCTGGTGTGCTCAAAATAAACGGGTATGGAGAACTAGTCCTTGATGCTTATAACATGGAGAGCACTCCTATTTGGTCTACTAATGTGTTGCAGTCGGTTCAAAATGTTAACACAAGTACTTTATCTGCACAGCTTTTAGATACAGGAAATCTAGTTGTGTTCCAGGATGATAATAATGAAAACTTTTTATGGCAAAGTTTTGATCATCCCACAGACACTTTAATTCCAGGTATGAAAGTTGGGGTGCATTGGAAAACAGGGCAAGAATGGGTTTTAACATCTTGGAAGTCACACGATGACCCTGCAATTGGGGACTATACCTTAAGGCTCTATCCAAATCAGATTGCATCCCCCGAATTTTTTATGTACAAGGGTTTGAGTAAGTATTGGCGAAGTCCAGGGCCAACGCCTAGTGCGGTCACTAATCAAGAGGAAACTTATTATTCCCTGGATGACACCAATGCAATTACAAGAATAACACTGTCTGATTCTGTGGAACGGCGCCTTACATGGGATGACGGTGGCCTTCAATGGAAGGAAGAATTTTCTGCACCGAAGTCCCGGTGTGACTGGTATGGACAGTGTGGTGCCAACAGCAAATGTTTCCCTGACAATGTTGGGCTGTTTGAGTGCGATTGTTTACCAGGGTATGAGCCTAGTTCTATAAATGATTGGAATCAGAAAAATGGGTCGGGTGGATGTGTGAGTAAGCGAGTTGGTGTATCAAAGTGCGGGAATAGAGATGGTTTTGTTAATGTGGCAAGAGTTAGTTGTCCAGACGCATCGATAGCATCACGGTTGAGAACAGGTATGAGTACCAAAGAGTGTGAGCAGGACTGCTTAAGAAATTGTTCTTGCACCGCATATATGACCATTGACAATGAAGGACTTGCGGATTGCTTGACATGGTACGATGACTTGATGGACATTTTAGAGTTCACAGAGTTTGGACACGATTTCTACGTTCGTGTGGATCACATCGAATTAGCTGAGAATGCTAGAAAATCAAAAGGTTTCTTGGAAAGAAGGGGTATGCTGGCTATTCCAATACTCTGTGCTCTGCTAGCATTTGTACTAATCATTATGTGCGCTTGCTCGTGGCACAAGAAAAAGAGGAATACAACGGAATTTGTGGAGGCGGATGAACTGGAGGAAACCAAGAGACACCCCGAATTGCAATTTTTTGATTTGAACACACTAATGTCAGCCACAGATAACTTCTCTCATGTCAATGAACTTGGCCAAGGTGGTTTTGGCTCTGTTTATAAGGGTCAGTTACCAAATGAGCAAAAGGTAGCTGTAAAAAGGTTGTCTAAAACATCAGGACAAGGGACTGAAGAATTCAAAAATGAAGTTGCACTTATAGCAGGACTTCAACACCGCAATCTTGTAAAGCTCTTAGGCTGCTGTATAAAGGGGGAAGAACGGATGTTAGTCCTGGAATACATGCCGAACAAAAGCTTGGACTCATTTCTCTTTGATCACAGAAGGCGGTCCTTCTTGGATTGGGAAAAGCGTTTTGAAATTATCAACGGGATTGCTCGTGGAATTCTATATCTTCACCAAGACTCAAGATTGAGGATCATCCATAGAGATCTAAAAACCAGCAATGTTCTTCTAGATTCTGAAATGAACCCAAAAATTTCCGACTTTGGCATGGCAAGAATATTTGATGGTGACCAAGTTCAGAATAAAACGAACCGAGTTGTTGGAACATATGGCTACATGTCACCAGAGTATGCAGTGTTTGGCAGATTTTCTACTAAATCAGATGTGTTTAGTTTTGGGATCATATTGTTAGAGGTTGTATCTGGCAAAAGAAACAATGGTTTTTCACAAGAGGATCCCTCCATGAACTTGATAAGACATGTTTGGGAACTCTGGAGAGAAGACAGAGCCTTAGAAATTGTGGATTCGACTCTGGAGTCATATAATTCTGATGAGGTCATGAGATGCATTCAAGTTGCACTCTTGTGCGTGCAAGAAGAATCAGAGGACCGGCCAGCCATGTCTGCCATTGTTTTCATGTTGAGTGGTGAAACACCTCCTCCAATATCTCCCAACCAACCTGCATATGTCTTCAGAAGAAACTCCAACACTGATGAGGAACCATCAGTACCAAAACGATTATATTCTATAAACGACTTGACAATGACTGCAATGGAAGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

822

Amino Acids

93.47

Weight (kDa)

5.39

Isoelectric Point (pI)

46.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 84 - 194 4.7e-36 D-mannose binding lectin
S_locus_glycop PF00954 240 - 318 2e-12 S-locus glycoprotein domain
PAN_2 PF08276 342 - 408 5.7e-16 PAN-like domain
Pkinase PF00069 506 - 771 5e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 508 - 773 3.1e-51 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 345, 1560
AasI GACNNNNNNGTC 1 cut(s) 2218
Acc36I ACCTGC 1 cut(s) 2371
AccB1I GGYRCC 2 cut(s) 795, 881
AccB7I CCANNNNNTGG 1 cut(s) 108
AccI GTMKAC 1 cut(s) 371
AciI CCGC 7 cut(s) 710, 1026, 1155, 1187, 1433, 1675, 1786
AclWI GGATC 5 cut(s) 1275, 1883, 2090, 2132, 2145
AcoI YGGCCR 2 cut(s) 1534, 2294
AcsI RAATTY 7 cut(s) 193, 650, 836, 1421, 1634, 1842, 1935
AcuI CTGAAG 4 cut(s) 146, 166, 1650, 2359
AcyI GRCGYC 1 cut(s) 796
AfaI GTAC 7 cut(s) 240, 412, 662, 1111, 1205, 1368, 2412
AfiI CCNNNNNNNGG 3 cut(s) 108, 602, 805
AflII CTTAAG 2 cut(s) 616, 1135
AflIII ACRYGT 2 cut(s) 2019, 2161
AgeI ACCGGT 1 cut(s) 204
AgsI TTSAA 8 cut(s) 52, 395, 824, 1486, 1639, 1670, 1817, 2255
AhlI ACTAGT 1 cut(s) 328
AjnI CCWGG 8 cut(s) 4, 92, 456, 520, 691, 741, 937, 1731
AjuI GAANNNNNNNTTGG 2 cut(s) 1739, 1771
AleI CACNNNNGTG 1 cut(s) 2266
AluBI AGCT 8 cut(s) 56, 201, 427, 1283, 1592, 1690, 1755, 2462
AluI AGCT 8 cut(s) 56, 201, 427, 1283, 1592, 1690, 1755, 2462
Alw21I GWGCWC 3 cut(s) 306, 359, 1354
Alw26I GTCTC 1 cut(s) 1450
AlwI GGATC 5 cut(s) 1275, 1883, 2090, 2132, 2145
AoxI GGCC 4 cut(s) 695, 817, 1534, 2294
ApeKI GCWGC 1 cut(s) 1698
ApoI RAATTY 7 cut(s) 193, 650, 836, 1421, 1634, 1842, 1935
AsiGI ACCGGT 1 cut(s) 204
Asp700I GAANNNNTTC 1 cut(s) 1144
AspLEI GCGC 2 cut(s) 798, 1385
AspS9I GGNCC 3 cut(s) 695, 1788, 2290
AsuC2I CCSGG 2 cut(s) 189, 856
AsuHPI GGTGA 4 cut(s) 1847, 1982, 2016, 2342
AsuII TTCGAA 1 cut(s) 168
AsuNHI GCTAGC 1 cut(s) 1357
AvaII GGWCC 2 cut(s) 1788, 2290
BaeI ACNNNNGTAYC 2 cut(s) 222, 255
BalI TGGCCA 1 cut(s) 1536
BamHI GGATCC 1 cut(s) 2137
BanI GGYRCC 2 cut(s) 795, 881
BauI CACGAG 2 cut(s) 1390, 1836
BbsI GAAGAC 2 cut(s) 2190, 2365
Bbv12I GWGCWC 3 cut(s) 306, 359, 1354
BbvI GCAGC 1 cut(s) 1685
BccI CCATC 6 cut(s) 128, 290, 1031, 1210, 1961, 2413
BceAI ACGGC 1 cut(s) 809
BcgI CGANNNNNNTGC 2 cut(s) 578, 612
BciT130I CCWGG 8 cut(s) 6, 94, 458, 522, 693, 743, 939, 1733
BclI TGATCA 2 cut(s) 496, 1774
BcnI CCSGG 2 cut(s) 189, 856
BcoDI GTCTC 1 cut(s) 1450
BcuI ACTAGT 1 cut(s) 328
BfaI CTAG 9 cut(s) 198, 234, 329, 446, 705, 951, 1293, 1358, 1913
BfoI RGCGCY 1 cut(s) 799
BfrI CTTAAG 2 cut(s) 616, 1135
BfuAI ACCTGC 1 cut(s) 2371
BglII AGATCT 1 cut(s) 1888
BisI GCNGC 1 cut(s) 1699
BlsI GCNGC 1 cut(s) 1700
BmcAI AGTACT 1 cut(s) 412
Bme18I GGWCC 2 cut(s) 1788, 2290
BmgT120I GGNCC 3 cut(s) 695, 1788, 2290
BmiI GGNNCC 5 cut(s) 113, 797, 883, 2139, 2403
BmsI GCATC 5 cut(s) 328, 650, 1085, 1094, 2237
BmtI GCTAGC 1 cut(s) 1361
BpiI GAAGAC 2 cut(s) 2190, 2365
BplI GAGNNNNNCTC 2 cut(s) 2126, 2158
BpmI CTGGAG 3 cut(s) 1463, 2197, 2237
Bpu14I TTCGAA 1 cut(s) 168
BpuEI CTTGAG 1 cut(s) 1849
BpuMI CCSGG 2 cut(s) 189, 856
Bsa29I ATCGAT 1 cut(s) 1079
BsaBI GATNNNNATC 1 cut(s) 969
BsaHI GRCGYC 1 cut(s) 796
BsaJI CCNNGG 7 cut(s) 5, 93, 187, 692, 741, 938, 1537
BsaWI WCCGGW 1 cut(s) 204
BsaXI ACNNNNNCTCC 6 cut(s) 132, 162, 315, 345, 2326, 2356
Bsc4I CCNNNNNNNGG 3 cut(s) 108, 602, 805
Bse118I RCCGGY 2 cut(s) 204, 2292
Bse1I ACTGG 2 cut(s) 869, 1446
Bse3DI GCAATG 2 cut(s) 1909, 2460
Bse8I GATNNNNATC 1 cut(s) 969
BseBI CCWGG 8 cut(s) 6, 94, 458, 522, 693, 743, 939, 1733
BseCI ATCGAT 1 cut(s) 1079
BseDI CCNNGG 7 cut(s) 5, 93, 187, 692, 741, 938, 1537
BseJI GATNNNNATC 1 cut(s) 969
BseLI CCNNNNNNNGG 3 cut(s) 108, 602, 805
BseMI GCAATG 2 cut(s) 1909, 2460
BseMII CTCAG 2 cut(s) 242, 1275
BseNI ACTGG 2 cut(s) 869, 1446
BseRI GAGGAG 1 cut(s) 2331
BseXI GCAGC 1 cut(s) 1685
BsgI GTGCAG 2 cut(s) 405, 828
BshFI GGCC 4 cut(s) 697, 819, 1536, 2296
BshNI GGYRCC 2 cut(s) 795, 881
BshTI ACCGGT 1 cut(s) 204
BshVI ATCGAT 1 cut(s) 1079
BsiHKAI GWGCWC 3 cut(s) 306, 359, 1354
BsiSI CCGG 4 cut(s) 189, 205, 856, 2293
BslFI GGGAC 4 cut(s) 110, 620, 838, 1639
BslI CCNNNNNNNGG 3 cut(s) 108, 602, 805
BsmAI GTCTC 1 cut(s) 1450
BsmFI GGGAC 4 cut(s) 110, 620, 838, 1639
BsmI GAATGC 2 cut(s) 1294, 2250
BsnI GGCC 4 cut(s) 697, 819, 1536, 2296
Bsp119I TTCGAA 1 cut(s) 168
Bsp1286I GDGCHC 3 cut(s) 306, 359, 1354
Bsp1407I TGTACA 1 cut(s) 660
Bsp143I GATC 7 cut(s) 496, 1267, 1774, 1875, 1888, 2082, 2137
BspACI CCGC 7 cut(s) 710, 1026, 1155, 1187, 1433, 1675, 1786
BspANI GGCC 4 cut(s) 697, 819, 1536, 2296
BspCNI CTCAG 2 cut(s) 241, 1276
BspDI ATCGAT 1 cut(s) 1079
BspHI TCATGA 1 cut(s) 2241
BspLI GGNNCC 5 cut(s) 113, 797, 883, 2139, 2403
BspMI ACCTGC 1 cut(s) 2371
BspOI GCTAGC 1 cut(s) 1361
BspPI GGATC 5 cut(s) 1275, 1883, 2090, 2132, 2145
BspT104I TTCGAA 1 cut(s) 168
BspT107I GGYRCC 2 cut(s) 795, 881
BspTI CTTAAG 2 cut(s) 616, 1135
BsrDI GCAATG 2 cut(s) 1909, 2460
BsrFI RCCGGY 2 cut(s) 204, 2292
BsrGI TGTACA 1 cut(s) 660
BsrI ACTGG 2 cut(s) 869, 1446
BssAI RCCGGY 2 cut(s) 204, 2292
BssECI CCNNGG 7 cut(s) 5, 93, 187, 692, 741, 938, 1537
BssMI GATC 7 cut(s) 496, 1267, 1774, 1875, 1888, 2082, 2137
BssNI GRCGYC 1 cut(s) 796
BssSI CACGAG 2 cut(s) 1390, 1836
BssT1I CCWWGG 1 cut(s) 1537
Bst2BI CACGAG 2 cut(s) 1390, 1836
Bst2UI CCWGG 8 cut(s) 6, 94, 458, 522, 693, 743, 939, 1733
Bst4CI ACNGT 5 cut(s) 253, 777, 815, 876, 1092
Bst6I CTCTTC 2 cut(s) 71, 80
BstACI GRCGYC 1 cut(s) 796
BstAFI CTTAAG 2 cut(s) 616, 1135
BstAUI TGTACA 1 cut(s) 660
BstBI TTCGAA 1 cut(s) 168
BstC8I GCNNGC 5 cut(s) 1332, 1359, 1387, 2273, 2298
BstDEI CTNAG 4 cut(s) 228, 1284, 1693, 2194
BstEII GGTNACC 1 cut(s) 1970
BstH2I RGCGCY 1 cut(s) 799
BstHHI GCGC 2 cut(s) 798, 1385
BstKTI GATC 7 cut(s) 499, 1270, 1777, 1878, 1891, 2085, 2140
BstMAI GTCTC 1 cut(s) 1450
BstMBI GATC 7 cut(s) 496, 1267, 1774, 1875, 1888, 2082, 2137
BstMWI GCNNNNNNNGC 6 cut(s) 1082, 1289, 1358, 1658, 2306, 2459
BstNI CCWGG 8 cut(s) 6, 94, 458, 522, 693, 743, 939, 1733
BstNSI RCATGY 3 cut(s) 1744, 2023, 2165
BstPI GGTNACC 1 cut(s) 1970
BstV1I GCAGC 1 cut(s) 1685
BstV2I GAAGAC 2 cut(s) 2190, 2365
BstX2I RGATCY 2 cut(s) 1888, 2137
BstYI RGATCY 2 cut(s) 1888, 2137
Bsu15I ATCGAT 1 cut(s) 1079
BsuRI GGCC 4 cut(s) 697, 819, 1536, 2296
BsuTUI ATCGAT 1 cut(s) 1079
BtsI GCAGTG 1 cut(s) 2043
BtsIMutI CAGTG 5 cut(s) 258, 773, 881, 2043, 2391
BveI ACCTGC 1 cut(s) 2371
Cac8I GCNNGC 5 cut(s) 1332, 1359, 1387, 2273, 2298
CciI TCATGA 1 cut(s) 2241
CfoI GCGC 2 cut(s) 798, 1385
Cfr10I RCCGGY 2 cut(s) 204, 2292
Cfr13I GGNCC 3 cut(s) 695, 1788, 2290
ClaI ATCGAT 1 cut(s) 1079
CseI GACGC 1 cut(s) 1082
Csp6I GTAC 7 cut(s) 239, 411, 661, 1110, 1204, 1367, 2411
CspAI ACCGGT 1 cut(s) 204
CviQI GTAC 7 cut(s) 239, 411, 661, 1110, 1204, 1367, 2411
DdeI CTNAG 4 cut(s) 228, 1284, 1693, 2194
DinI GGCGCC 1 cut(s) 797
DpnI GATC 7 cut(s) 498, 1269, 1776, 1877, 1890, 2084, 2139
DpnII GATC 7 cut(s) 496, 1267, 1774, 1875, 1888, 2082, 2137
DrdI GACNNNNNNGTC 1 cut(s) 2218
DseDI GACNNNNNNGTC 1 cut(s) 2218
EaeI YGGCCR 2 cut(s) 1534, 2294
Eam1104I CTCTTC 2 cut(s) 71, 80
EarI CTCTTC 2 cut(s) 71, 80
EciI GGCGGA 1 cut(s) 1448
Eco130I CCWWGG 1 cut(s) 1537
Eco47I GGWCC 2 cut(s) 1788, 2290
Eco57I CTGAAG 4 cut(s) 146, 166, 1650, 2359
Eco91I GGTNACC 1 cut(s) 1970
EcoO65I GGTNACC 1 cut(s) 1970
EcoRI GAATTC 2 cut(s) 1634, 1842
EcoRII CCWGG 8 cut(s) 4, 92, 456, 520, 691, 741, 937, 1731
EcoT14I CCWWGG 1 cut(s) 1537
EcoT22I ATGCAT 1 cut(s) 2252
EgeI GGCGCC 1 cut(s) 797
EheI GGCGCC 1 cut(s) 797
ErhI CCWWGG 1 cut(s) 1537
FalI AAGNNNNNCTT 4 cut(s) 45, 77, 1638, 1670
FaqI GGGAC 4 cut(s) 110, 620, 838, 1639
FauI CCCGC 1 cut(s) 1019
FauNDI CATATG 2 cut(s) 2011, 2368
FbaI TGATCA 2 cut(s) 496, 1774
FblI GTMKAC 1 cut(s) 371
Fnu4HI GCNGC 1 cut(s) 1699
Fsp4HI GCNGC 1 cut(s) 1699
FspBI CTAG 9 cut(s) 198, 234, 329, 446, 705, 951, 1293, 1358, 1913
GlaI GCGC 2 cut(s) 797, 1384
GluI GCNGC 1 cut(s) 1699
GsuI CTGGAG 3 cut(s) 1463, 2197, 2237
HaeII RGCGCY 1 cut(s) 799
HaeIII GGCC 4 cut(s) 697, 819, 1536, 2296
HapII CCGG 4 cut(s) 189, 205, 856, 2293
HgaI GACGC 1 cut(s) 1082
HhaI GCGC 2 cut(s) 798, 1385
Hin1I GRCGYC 1 cut(s) 796
Hin6I GCGC 2 cut(s) 796, 1383
HinP1I GCGC 2 cut(s) 796, 1383
HincII GTYRAC 1 cut(s) 403
HindII GTYRAC 1 cut(s) 403
HindIII AAGCTT 1 cut(s) 1753
HpaI GTTAAC 1 cut(s) 403
HpaII CCGG 4 cut(s) 189, 205, 856, 2293
HphI GGTGA 4 cut(s) 1847, 1982, 2016, 2342
Hpy166II GTNNAC 4 cut(s) 372, 403, 935, 1233
Hpy188III TCNNGA 8 cut(s) 722, 1070, 1617, 1866, 1913, 2176, 2216, 2242
Hpy8I GTNNAC 4 cut(s) 372, 403, 935, 1233
HpyAV CCTTC 8 cut(s) 80, 121, 823, 830, 1172, 1314, 1776, 1801
HpyCH4III ACNGT 5 cut(s) 253, 777, 815, 876, 1092
HpyCH4IV ACGT 1 cut(s) 1257
HpyF10VI GCNNNNNNNGC 6 cut(s) 1082, 1289, 1358, 1658, 2306, 2459
HpyF3I CTNAG 4 cut(s) 228, 1284, 1693, 2194
HpySE526I ACGT 1 cut(s) 1257
Hsp92I GRCGYC 1 cut(s) 796
HspAI GCGC 2 cut(s) 796, 1383
KasI GGCGCC 1 cut(s) 795
Ksp22I TGATCA 2 cut(s) 496, 1774
KspAI GTTAAC 1 cut(s) 403
Kzo9I GATC 7 cut(s) 496, 1267, 1774, 1875, 1888, 2082, 2137
Lsp1109I GCAGC 1 cut(s) 1685
LweI GCATC 5 cut(s) 328, 650, 1085, 1094, 2237
MaeI CTAG 9 cut(s) 198, 234, 329, 446, 705, 951, 1293, 1358, 1913
MaeII ACGT 1 cut(s) 1257
MaeIII GTNAC 6 cut(s) 582, 712, 860, 1569, 1970, 2022
MalI GATC 7 cut(s) 498, 1269, 1776, 1877, 1890, 2084, 2139
MboI GATC 7 cut(s) 496, 1267, 1774, 1875, 1888, 2082, 2137
MfeI CAATTG 1 cut(s) 600
MflI RGATCY 2 cut(s) 1888, 2137
MhlI GDGCHC 3 cut(s) 306, 359, 1354
MlsI TGGCCA 1 cut(s) 1536
MluNI TGGCCA 1 cut(s) 1536
Mly113I GGCGCC 1 cut(s) 796
MlyI GAGTC 4 cut(s) 1754, 1856, 2206, 2228
MmeI TCCRAC 4 cut(s) 193, 1965, 1984, 2412
Mox20I TGGCCA 1 cut(s) 1536
Mph1103I ATGCAT 1 cut(s) 2252
MroXI GAANNNNTTC 1 cut(s) 1144
MscI TGGCCA 1 cut(s) 1536
MseI TTAA 6 cut(s) 402, 515, 569, 617, 1047, 1136
MslI CAYNNNNRTG 3 cut(s) 1493, 1524, 2266
Msp20I TGGCCA 1 cut(s) 1536
MspCI CTTAAG 2 cut(s) 616, 1135
MspI CCGG 4 cut(s) 189, 205, 856, 2293
MunI CAATTG 1 cut(s) 600
Mva1269I GAATGC 2 cut(s) 1294, 2250
MvaI CCWGG 8 cut(s) 6, 94, 458, 522, 693, 743, 939, 1733
MwoI GCNNNNNNNGC 6 cut(s) 1082, 1289, 1358, 1658, 2306, 2459
NarI GGCGCC 1 cut(s) 796
NciI CCSGG 2 cut(s) 189, 856
NdeI CATATG 2 cut(s) 2011, 2368
NdeII GATC 7 cut(s) 496, 1267, 1774, 1875, 1888, 2082, 2137
NheI GCTAGC 1 cut(s) 1357
NlaIV GGNNCC 5 cut(s) 113, 797, 883, 2139, 2403
NmuCI GTSAC 5 cut(s) 582, 712, 860, 1970, 2022
NsiI ATGCAT 1 cut(s) 2252
NspI RCATGY 3 cut(s) 1744, 2023, 2165
NspV TTCGAA 1 cut(s) 168
OliI CACNNNNGTG 1 cut(s) 2266
PagI TCATGA 1 cut(s) 2241
PciI ACATGT 2 cut(s) 2019, 2161
PcsI WCGNNNNNNNCGW 1 cut(s) 1254
PctI GAATGC 2 cut(s) 1294, 2250
PdmI GAANNNNTTC 1 cut(s) 1144
PfeI GAWTC 7 cut(s) 89, 216, 782, 970, 1916, 2207, 2282
PflMI CCANNNNNTGG 1 cut(s) 108
PfoI TCCNGGA 2 cut(s) 456, 1731
PinAI ACCGGT 1 cut(s) 204
PkrI GCNGC 1 cut(s) 1700
PleI GAGTC 4 cut(s) 1754, 1856, 2206, 2227
PluTI GGCGCC 1 cut(s) 799
PpsI GAGTC 4 cut(s) 1754, 1856, 2206, 2227
PscI ACATGT 2 cut(s) 2019, 2161
PsiI TTATAA 2 cut(s) 345, 1560
Psp6I CCWGG 8 cut(s) 4, 92, 456, 520, 691, 741, 937, 1731
PspEI GGTNACC 1 cut(s) 1970
PspGI CCWGG 8 cut(s) 4, 92, 456, 520, 691, 741, 937, 1731
PspN4I GGNNCC 5 cut(s) 113, 797, 883, 2139, 2403
PspPI GGNCC 3 cut(s) 695, 1788, 2290
PsrI GAACNNNNNNTAC 2 cut(s) 2394, 2426
PsuI RGATCY 2 cut(s) 1888, 2137
RsaI GTAC 7 cut(s) 240, 412, 662, 1111, 1205, 1368, 2412
RsaNI GTAC 7 cut(s) 239, 411, 661, 1110, 1204, 1367, 2411
RseI CAYNNNNRTG 3 cut(s) 1493, 1524, 2266
SaqAI TTAA 6 cut(s) 402, 515, 569, 617, 1047, 1136
SatI GCNGC 1 cut(s) 1699
Sau3AI GATC 7 cut(s) 496, 1267, 1774, 1875, 1888, 2082, 2137
Sau96I GGNCC 3 cut(s) 695, 1788, 2290
ScaI AGTACT 1 cut(s) 412
SchI GAGTC 4 cut(s) 1754, 1856, 2206, 2228
SduI GDGCHC 3 cut(s) 306, 359, 1354
SfaNI GCATC 5 cut(s) 328, 650, 1085, 1094, 2237
SfoI GGCGCC 1 cut(s) 797
SfuI TTCGAA 1 cut(s) 168
SinI GGWCC 2 cut(s) 1788, 2290
SmiMI CAYNNNNRTG 3 cut(s) 1493, 1524, 2266
SmlI CTYRAG 3 cut(s) 616, 1135, 1864
SmoI CTYRAG 3 cut(s) 616, 1135, 1864
SpeI ACTAGT 1 cut(s) 328
SsiI CCGC 7 cut(s) 710, 1026, 1155, 1187, 1433, 1675, 1786
SspDI GGCGCC 1 cut(s) 795
SspI AATATT 1 cut(s) 1962
SspMI CTAG 9 cut(s) 198, 234, 329, 446, 705, 951, 1293, 1358, 1913
StyI CCWWGG 1 cut(s) 1537
TaaI ACNGT 5 cut(s) 253, 777, 815, 876, 1092
TaiI ACGT 1 cut(s) 1260
TaqI TCGA 5 cut(s) 168, 1079, 1275, 2210, 2464
TatI WGTACW 3 cut(s) 410, 660, 1366
TfiI GAWTC 7 cut(s) 89, 216, 782, 970, 1916, 2207, 2282
Tru1I TTAA 6 cut(s) 402, 515, 569, 617, 1047, 1136
Tru9I TTAA 6 cut(s) 402, 515, 569, 617, 1047, 1136
TscAI CASTG 5 cut(s) 258, 780, 881, 2043, 2398
TseFI GTSAC 5 cut(s) 582, 712, 860, 1970, 2022
TseI GCWGC 1 cut(s) 1698
Tsp45I GTSAC 5 cut(s) 582, 712, 860, 1970, 2022
TspDTI ATGAA 9 cut(s) 486, 542, 1191, 1452, 1542, 1659, 1940, 2162, 2308
TspGWI ACGGA 2 cut(s) 1433, 1735
TspRI CASTG 5 cut(s) 258, 780, 881, 2043, 2398
Van91I CCANNNNNTGG 1 cut(s) 108
Vha464I CTTAAG 2 cut(s) 616, 1135
VpaK11BI GGWCC 2 cut(s) 1788, 2290
XapI RAATTY 7 cut(s) 193, 650, 836, 1421, 1634, 1842, 1935
XbaI TCTAGA 1 cut(s) 1912
XceI RCATGY 3 cut(s) 1744, 2023, 2165
XcmI CCANNNNNNNNNTGG 1 cut(s) 1544
XmiI GTMKAC 1 cut(s) 371
XmnI GAANNNNTTC 1 cut(s) 1144
XspI CTAG 9 cut(s) 198, 234, 329, 446, 705, 951, 1293, 1358, 1913
ZrmI AGTACT 1 cut(s) 412
Zsp2I ATGCAT 1 cut(s) 2252
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.