Prupe.4G032100_v2.0.a1

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000112
Physical Location & Seq
Forward (+)
1490997 .. 1494593
3597 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G032100.1

Sequence Viewer

Length: 1947 bp
ATGCTTTTCAAAGCTTTGCTTGTGTTCTTCCTCCTCCTCTTCCCATTTTGCACATCCATTGACACCATAGCACTGAACCAGCGTGTGAAAGATGGTGACTTTATAGTGTCCAAAGAAAACAACTTTGAATTAGGCTTCTTCAGCCCCGGCAATTCGAGCTCTCGCTATGTTGGAATTTGGTATGCTAATAAGTCTGAAAAGGCAGTGGTTTGGGTTGCAAACAGAAACAACCCCATCAATGATACCTCTGGCGTCCTCACCATCAACAGATGGGTGTTGACGGATTATGGGTTAAATCAACAACTGAGATGGAGTGATGCTGTTAATCAATGGGAGGAACTATGGGCTGCACCAAAATACCGGTGTGACCAATATGGACAGTGTGGTGCCAACAGCAAATGTAGCCCTGACAACGTTAATATGTTTGAGTGTGAGTGTCTACCAGGGTACGAACCAAAATCTTTGAATGATTGGAACAGGAGAGATGGGTCAGAGGGGTGTGTGAGTAAGCGAATTGGGCTGCCAAAATGTGGGAATGGGGAAGGGTTTGTGAAGGTGGCACGGGTGAAAGAGCCAGACACAACAAAAGCAGCACAATTGTTGACAAGTATTAGTGCCAATCAGTGTCAGCAGGTGTGCTTAAGCAATTGTTCCTGCACCGCGTATATGAGCATAGAATGGGAAGGCAGCATTGATTGCTTGGTATGGTACGGTGAGTTGCTGGACATTTTAGTCCGCACAGAGCTAGGACAAGATCTGTATGTTCGTGTGGACAAAATGGAATTAGCTGAGAATGCCAGAAAATCGGAAGGTTTTCTCAAAAGGAGGGGTTTGCTGGCTATTCTAATAGTGGCTGTTCTTCTGGCATTGGTACTAATCATTGTGTTTGCCTGCTGGTGGCTTAATAAAAAGAGGAAGATAAAAGACTTTGTGGAGGCCGATGAACTTGAGGAAAGTAGGAGACACCCAGAGCTGCAATTTTTCCATCTGAGTACAATAATAGAAGCTACAGACAACTTCTCTCCTGTCAATAAACTTGGCCAAGGTGGTTTTGGCACTGTTTATAAGGGTCACCTACAAAATGATCAGAAGATTGCTGTAAAAAGACTCTGCAAAACTTCAGGACAAGGAATTGAAGAATTCAAAAATGAAGTTGCGCTGATAGCCAAACTTCAACACAGGAATCTTGTGAAACTTTTAGGATGCTGTATACAGGGAGAGGAAAGGATGTTAGTCCTTGAATACTTGCCAAACAAAAGCTTGGACTTCTTTGTTTTCGATCACTCAAGAAGGTCCTTGTTGGATTGGAAAAAGCGGTTTGAAATTATCAATGGGGTTGCCCGTGGGGTTCTGTATCTTCACCAGGATTCAAGGCTTCGGATTATCCACAGGGATCTAAAAACCAGCAATGTTCTTCTAGATGCTGAGATGAACCCAAAAATTTCAGATTTCGGAATGGCAAGAATATTCCATGGGGACCAACTGCAGGATAAAACCAACAGAGTTGTTGGAACATATGGATATATGTCACCAGAGTATGCAGTGTTTGGAAGATATTCAACCAAATCAGATGTCTTTAGTTTTGGGATTGTATTATTGGAGATTGTGAGTGGCAAGAAAAATAATGGCTCTTATCAAGAGGACCATTCCATGAATTTGATAGGATATGTTTGGCAGCTGTGGAGAGAAGACAGGGCCTTAGAAATTGTGGATTCATTACTGGAGTCATATCATTCTGATGAAGTCATGAGATGCATTCAAGTCGGGCTCTTGTGCGTGCAAGAAGACTCAAAGGACCGACCAACCATGTCAGCCGTTGTGTTTATGTTGAGTGGTGAAGCATCTCTACCATCTCCTCAGCAGCCTGCATTTGTCTTCAGAAAAAGTTCCTGTCTGCATTTATATTCGGTTAATGACTTGACAATAACCAAAGTTGAAGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

649

Amino Acids

73.75

Weight (kDa)

6.83

Isoelectric Point (pI)

44.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1065
AarI CACCTGC 1 cut(s) 622
AasI GACNNNNNNGTC 1 cut(s) 731
Acc36I ACCTGC 1 cut(s) 622
AccB1I GGYRCC 1 cut(s) 386
AccB7I CCANNNNNTGG 1 cut(s) 530
AccI GTMKAC 2 cut(s) 439, 1210
AccII CGCG 1 cut(s) 662
AciI CCGC 3 cut(s) 660, 736, 1315
AclI AACGTT 1 cut(s) 414
AclWI GGATC 1 cut(s) 1401
AcoI YGGCCR 1 cut(s) 1039
AcsI RAATTY 4 cut(s) 174, 1139, 1440, 1654
AcuI CTGAAG 3 cut(s) 124, 1104, 1861
AcyI GRCGYC 1 cut(s) 252
AfaI GTAC 4 cut(s) 449, 710, 873, 994
AfiI CCNNNNNNNGG 3 cut(s) 530, 897, 1486
AflII CTTAAG 1 cut(s) 640
AgeI ACCGGT 1 cut(s) 360
AjnI CCWGG 2 cut(s) 442, 1362
AluBI AGCT 9 cut(s) 14, 159, 745, 788, 973, 1007, 1260, 1678, 1940
AluI AGCT 9 cut(s) 14, 159, 745, 788, 973, 1007, 1260, 1678, 1940
Alw21I GWGCWC 1 cut(s) 161
Alw26I GTCTC 1 cut(s) 955
AlwI GGATC 1 cut(s) 1401
AoxI GGCC 3 cut(s) 936, 1039, 1695
ApeKI GCWGC 7 cut(s) 347, 520, 590, 687, 973, 1675, 1861
ApoI RAATTY 4 cut(s) 174, 1139, 1440, 1654
ArsI GACNNNNNNTTYG 2 cut(s) 1557, 1589
AsiGI ACCGGT 1 cut(s) 360
Asp700I GAANNNNTTC 3 cut(s) 813, 1555, 1885
AspLEI GCGC 1 cut(s) 1159
AspS9I GGNCC 5 cut(s) 1293, 1477, 1642, 1695, 1795
AsuC2I CCSGG 1 cut(s) 147
AsuHPI GGTGA 8 cut(s) 107, 250, 577, 725, 1064, 1352, 1521, 1847
AvaII GGWCC 4 cut(s) 1293, 1477, 1642, 1795
BalI TGGCCA 1 cut(s) 1041
BanI GGYRCC 1 cut(s) 386
BanII GRGCYC 2 cut(s) 161, 1770
BarI GAAGNNNNNNTAC 2 cut(s) 1830, 1862
BbsI GAAGAC 3 cut(s) 1695, 1791, 1867
Bbv12I GWGCWC 1 cut(s) 161
BbvCI CCTCAGC 1 cut(s) 1857
BbvI GCAGC 7 cut(s) 334, 507, 602, 699, 960, 1687, 1873
BccI CCATC 8 cut(s) 86, 242, 264, 269, 303, 479, 993, 1858
BceAI ACGGC 1 cut(s) 1799
BcgI CGANNNNNNTGC 2 cut(s) 1744, 1778
BciT130I CCWGG 2 cut(s) 444, 1364
BclI TGATCA 1 cut(s) 1084
BcnI CCSGG 1 cut(s) 147
BcoDI GTCTC 1 cut(s) 955
BfaI CTAG 2 cut(s) 746, 1418
BfmI CTRYAG 2 cut(s) 1008, 1484
BfrI CTTAAG 1 cut(s) 640
BfuAI ACCTGC 1 cut(s) 622
BglII AGATCT 1 cut(s) 754
BisI GCNGC 7 cut(s) 348, 521, 591, 688, 974, 1676, 1862
BlsI GCNGC 7 cut(s) 349, 522, 592, 689, 975, 1677, 1863
Bme1390I CCNGG 3 cut(s) 147, 444, 1364
Bme18I GGWCC 4 cut(s) 1293, 1477, 1642, 1795
BmgT120I GGNCC 5 cut(s) 1293, 1477, 1642, 1695, 1795
BmiI GGNNCC 2 cut(s) 388, 1478
BmrFI CCNGG 3 cut(s) 147, 444, 1364
BmsI GCATC 5 cut(s) 307, 1193, 1411, 1742, 1850
BpiI GAAGAC 3 cut(s) 1695, 1791, 1867
BpmI CTGGAG 1 cut(s) 1742
Bpu10I CCTNAGC 1 cut(s) 1857
BpuEI CTTGAG 2 cut(s) 968, 1270
BpuMI CCSGG 1 cut(s) 147
BsaHI GRCGYC 1 cut(s) 252
BsaJI CCNNGG 5 cut(s) 145, 443, 1042, 1342, 1471
BsaWI WCCGGW 1 cut(s) 360
Bsc4I CCNNNNNNNGG 3 cut(s) 530, 897, 1486
Bse118I RCCGGY 1 cut(s) 360
Bse1I ACTGG 1 cut(s) 1725
Bse3DI GCAATG 1 cut(s) 1414
BseBI CCWGG 2 cut(s) 444, 1364
BseDI CCNNGG 5 cut(s) 145, 443, 1042, 1342, 1471
BseGI GGATG 3 cut(s) 53, 1208, 1233
BseLI CCNNNNNNNGG 3 cut(s) 530, 897, 1486
BseMI GCAATG 1 cut(s) 1414
BseMII CTCAG 5 cut(s) 296, 780, 980, 1416, 1871
BseNI ACTGG 1 cut(s) 1725
BseRI GAGGAG 3 cut(s) 23, 26, 1845
BseXI GCAGC 7 cut(s) 334, 507, 602, 699, 960, 1687, 1873
BsgI GTGCAG 2 cut(s) 333, 640
Bsh1236I CGCG 1 cut(s) 662
BshFI GGCC 3 cut(s) 938, 1041, 1697
BshNI GGYRCC 1 cut(s) 386
BshTI ACCGGT 1 cut(s) 360
BsiHKAI GWGCWC 1 cut(s) 161
BsiSI CCGG 2 cut(s) 147, 361
BslFI GGGAC 1 cut(s) 1490
BslI CCNNNNNNNGG 3 cut(s) 530, 897, 1486
BsmAI GTCTC 1 cut(s) 955
BsmFI GGGAC 1 cut(s) 1490
BsmI GAATGC 2 cut(s) 799, 1755
BsnI GGCC 3 cut(s) 938, 1041, 1697
Bsp1286I GDGCHC 2 cut(s) 161, 1770
Bsp143I GATC 4 cut(s) 754, 1084, 1279, 1393
Bsp19I CCATGG 1 cut(s) 1471
BspACI CCGC 3 cut(s) 660, 736, 1315
BspANI GGCC 3 cut(s) 938, 1041, 1697
BspCNI CTCAG 5 cut(s) 297, 781, 981, 1417, 1870
BspFNI CGCG 1 cut(s) 662
BspHI TCATGA 1 cut(s) 1746
BspLI GGNNCC 2 cut(s) 388, 1478
BspMAI CTGCAG 1 cut(s) 1488
BspMI ACCTGC 1 cut(s) 622
BspPI GGATC 1 cut(s) 1401
BspT107I GGYRCC 1 cut(s) 386
BspTI CTTAAG 1 cut(s) 640
BsrDI GCAATG 1 cut(s) 1414
BsrFI RCCGGY 1 cut(s) 360
BsrI ACTGG 1 cut(s) 1725
BssAI RCCGGY 1 cut(s) 360
BssECI CCNNGG 5 cut(s) 145, 443, 1042, 1342, 1471
BssMI GATC 4 cut(s) 754, 1084, 1279, 1393
BssNAI GTATAC 1 cut(s) 1211
BssNI GRCGYC 1 cut(s) 252
BssT1I CCWWGG 2 cut(s) 1042, 1471
Bst1107I GTATAC 1 cut(s) 1211
Bst2UI CCWGG 2 cut(s) 444, 1364
Bst4CI ACNGT 3 cut(s) 381, 713, 1060
Bst6I CTCTTC 1 cut(s) 44
BstACI GRCGYC 1 cut(s) 252
BstAFI CTTAAG 1 cut(s) 640
BstAPI GCANNNNNTGC 1 cut(s) 696
BstC8I GCNNGC 4 cut(s) 837, 892, 1778, 1866
BstDEI CTNAG 6 cut(s) 305, 789, 989, 1425, 1699, 1857
BstDSI CCRYGG 2 cut(s) 1342, 1471
BstEII GGTNACC 1 cut(s) 1070
BstF5I GGATG 3 cut(s) 53, 1208, 1233
BstFNI CGCG 1 cut(s) 662
BstHHI GCGC 1 cut(s) 1159
BstKTI GATC 4 cut(s) 757, 1087, 1282, 1396
BstMAI GTCTC 1 cut(s) 955
BstMBI GATC 4 cut(s) 754, 1084, 1279, 1393
BstMWI GCNNNNNNNGC 7 cut(s) 141, 156, 402, 517, 696, 794, 1163
BstNI CCWGG 2 cut(s) 444, 1364
BstPI GGTNACC 1 cut(s) 1070
BstSCI CCNGG 3 cut(s) 145, 442, 1362
BstSFI CTRYAG 2 cut(s) 1008, 1484
BstUI CGCG 1 cut(s) 662
BstV1I GCAGC 7 cut(s) 334, 507, 602, 699, 960, 1687, 1873
BstV2I GAAGAC 3 cut(s) 1695, 1791, 1867
BstX2I RGATCY 2 cut(s) 754, 1393
BstYI RGATCY 2 cut(s) 754, 1393
BstZ17I GTATAC 1 cut(s) 1211
BsuRI GGCC 3 cut(s) 938, 1041, 1697
BtgI CCRYGG 2 cut(s) 1342, 1471
BtsCI GGATG 3 cut(s) 53, 1208, 1233
BtsI GCAGTG 2 cut(s) 210, 1548
BtsIMutI CAGTG 6 cut(s) 71, 210, 386, 629, 1056, 1548
BveI ACCTGC 1 cut(s) 622
Cac8I GCNNGC 4 cut(s) 837, 892, 1778, 1866
CciI TCATGA 1 cut(s) 1746
CfoI GCGC 1 cut(s) 1159
Cfr10I RCCGGY 1 cut(s) 360
Cfr13I GGNCC 5 cut(s) 1293, 1477, 1642, 1695, 1795
CseI GACGC 1 cut(s) 241
Csp6I GTAC 4 cut(s) 448, 709, 872, 993
CspAI ACCGGT 1 cut(s) 360
CviAII CATG 4 cut(s) 1472, 1651, 1747, 1807
CviQI GTAC 4 cut(s) 448, 709, 872, 993
DdeI CTNAG 6 cut(s) 305, 789, 989, 1425, 1699, 1857
DpnI GATC 4 cut(s) 756, 1086, 1281, 1395
DpnII GATC 4 cut(s) 754, 1084, 1279, 1393
DrdI GACNNNNNNGTC 1 cut(s) 731
DseDI GACNNNNNNGTC 1 cut(s) 731
EaeI YGGCCR 1 cut(s) 1039
Eam1104I CTCTTC 1 cut(s) 44
EarI CTCTTC 1 cut(s) 44
Ecl136II GAGCTC 1 cut(s) 159
Eco130I CCWWGG 2 cut(s) 1042, 1471
Eco24I GRGCYC 2 cut(s) 161, 1770
Eco47I GGWCC 4 cut(s) 1293, 1477, 1642, 1795
Eco53kI GAGCTC 1 cut(s) 159
Eco57I CTGAAG 3 cut(s) 124, 1104, 1861
Eco91I GGTNACC 1 cut(s) 1070
EcoICRI GAGCTC 1 cut(s) 159
EcoO109I RGGNCCY 2 cut(s) 1293, 1695
EcoO65I GGTNACC 1 cut(s) 1070
EcoRI GAATTC 1 cut(s) 1139
EcoRII CCWGG 2 cut(s) 442, 1362
EcoT14I CCWWGG 2 cut(s) 1042, 1471
EcoT22I ATGCAT 1 cut(s) 1757
EcoT38I GRGCYC 2 cut(s) 161, 1770
ErhI CCWWGG 2 cut(s) 1042, 1471
FaeI CATG 4 cut(s) 1475, 1654, 1750, 1810
FalI AAGNNNNNCTT 1 cut(s) 35
FaqI GGGAC 1 cut(s) 1490
FatI CATG 4 cut(s) 1471, 1650, 1746, 1806
FauNDI CATATG 1 cut(s) 1516
FbaI TGATCA 1 cut(s) 1084
FblI GTMKAC 2 cut(s) 439, 1210
Fnu4HI GCNGC 7 cut(s) 348, 521, 591, 688, 974, 1676, 1862
FokI GGATG 3 cut(s) 40, 1215, 1240
FriOI GRGCYC 2 cut(s) 161, 1770
Fsp4HI GCNGC 7 cut(s) 348, 521, 591, 688, 974, 1676, 1862
FspBI CTAG 2 cut(s) 746, 1418
GlaI GCGC 1 cut(s) 1158
GluI GCNGC 7 cut(s) 348, 521, 591, 688, 974, 1676, 1862
GsuI CTGGAG 1 cut(s) 1742
HaeIII GGCC 3 cut(s) 938, 1041, 1697
HapII CCGG 2 cut(s) 147, 361
HgaI GACGC 1 cut(s) 241
HhaI GCGC 1 cut(s) 1159
Hin1I GRCGYC 1 cut(s) 252
Hin1II CATG 4 cut(s) 1475, 1654, 1750, 1810
Hin6I GCGC 1 cut(s) 1157
HinP1I GCGC 1 cut(s) 1157
HincII GTYRAC 2 cut(s) 279, 603
HindII GTYRAC 2 cut(s) 279, 603
HindIII AAGCTT 2 cut(s) 12, 1258
HinfI GANTC 6 cut(s) 1107, 1183, 1367, 1712, 1724, 1787
HpaII CCGG 2 cut(s) 147, 361
HphI GGTGA 8 cut(s) 107, 250, 577, 725, 1064, 1352, 1521, 1847
Hpy166II GTNNAC 5 cut(s) 279, 440, 603, 772, 1211
Hpy188III TCNNGA 5 cut(s) 1122, 1287, 1418, 1637, 1747
Hpy8I GTNNAC 5 cut(s) 279, 440, 603, 772, 1211
HpyAV CCTTC 5 cut(s) 536, 547, 677, 803, 1284
HpyCH4III ACNGT 3 cut(s) 381, 713, 1060
HpyCH4IV ACGT 1 cut(s) 414
HpyF10VI GCNNNNNNNGC 7 cut(s) 141, 156, 402, 517, 696, 794, 1163
HpyF3I CTNAG 6 cut(s) 305, 789, 989, 1425, 1699, 1857
HpySE526I ACGT 1 cut(s) 414
Hsp92I GRCGYC 1 cut(s) 252
Hsp92II CATG 4 cut(s) 1475, 1654, 1750, 1810
HspAI GCGC 1 cut(s) 1157
Ksp22I TGATCA 1 cut(s) 1084
Kzo9I GATC 4 cut(s) 754, 1084, 1279, 1393
Lsp1109I GCAGC 7 cut(s) 334, 507, 602, 699, 960, 1687, 1873
LweI GCATC 5 cut(s) 307, 1193, 1411, 1742, 1850
MaeI CTAG 2 cut(s) 746, 1418
MaeII ACGT 1 cut(s) 414
MaeIII GTNAC 4 cut(s) 95, 365, 1070, 1527
MalI GATC 4 cut(s) 756, 1086, 1281, 1395
MboI GATC 4 cut(s) 754, 1084, 1279, 1393
MfeI CAATTG 2 cut(s) 596, 646
MflI RGATCY 2 cut(s) 754, 1393
MhlI GDGCHC 2 cut(s) 161, 1770
MlsI TGGCCA 1 cut(s) 1041
MluNI TGGCCA 1 cut(s) 1041
MlyI GAGTC 3 cut(s) 1101, 1733, 1781
MmeI TCCRAC 3 cut(s) 151, 1281, 1489
Mox20I TGGCCA 1 cut(s) 1041
Mph1103I ATGCAT 1 cut(s) 1757
MroXI GAANNNNTTC 3 cut(s) 813, 1555, 1885
MscI TGGCCA 1 cut(s) 1041
MseI TTAA 6 cut(s) 293, 324, 417, 641, 903, 1911
MslI CAYNNNNRTG 1 cut(s) 1737
Msp20I TGGCCA 1 cut(s) 1041
MspA1I CMGCKG 1 cut(s) 1678
MspCI CTTAAG 1 cut(s) 640
MspI CCGG 2 cut(s) 147, 361
MspR9I CCNGG 3 cut(s) 147, 444, 1364
MunI CAATTG 2 cut(s) 596, 646
Mva1269I GAATGC 2 cut(s) 799, 1755
MvaI CCWGG 2 cut(s) 444, 1364
MvnI CGCG 1 cut(s) 662
MwoI GCNNNNNNNGC 7 cut(s) 141, 156, 402, 517, 696, 794, 1163
NciI CCSGG 1 cut(s) 147
NcoI CCATGG 1 cut(s) 1471
NdeI CATATG 1 cut(s) 1516
NdeII GATC 4 cut(s) 754, 1084, 1279, 1393
NlaIII CATG 4 cut(s) 1475, 1654, 1750, 1810
NlaIV GGNNCC 2 cut(s) 388, 1478
NmuCI GTSAC 4 cut(s) 95, 365, 1070, 1527
NsiI ATGCAT 1 cut(s) 1757
PagI TCATGA 1 cut(s) 1746
PaqCI CACCTGC 1 cut(s) 622
PctI GAATGC 2 cut(s) 799, 1755
PdmI GAANNNNTTC 3 cut(s) 813, 1555, 1885
PfeI GAWTC 3 cut(s) 1183, 1367, 1712
PflMI CCANNNNNTGG 1 cut(s) 530
PinAI ACCGGT 1 cut(s) 360
PkrI GCNGC 7 cut(s) 349, 522, 592, 689, 975, 1677, 1863
PleI GAGTC 3 cut(s) 1101, 1732, 1781
PpsI GAGTC 3 cut(s) 1101, 1732, 1781
PpuMI RGGWCCY 1 cut(s) 1293
PsiI TTATAA 1 cut(s) 1065
Psp124BI GAGCTC 1 cut(s) 161
Psp1406I AACGTT 1 cut(s) 414
Psp5II RGGWCCY 1 cut(s) 1293
Psp6I CCWGG 2 cut(s) 442, 1362
PspEI GGTNACC 1 cut(s) 1070
PspGI CCWGG 2 cut(s) 442, 1362
PspN4I GGNNCC 2 cut(s) 388, 1478
PspPI GGNCC 5 cut(s) 1293, 1477, 1642, 1695, 1795
PspPPI RGGWCCY 1 cut(s) 1293
PstI CTGCAG 1 cut(s) 1488
PsuI RGATCY 2 cut(s) 754, 1393
PvuII CAGCTG 1 cut(s) 1678
RsaI GTAC 4 cut(s) 449, 710, 873, 994
RsaNI GTAC 4 cut(s) 448, 709, 872, 993
RseI CAYNNNNRTG 1 cut(s) 1737
SacI GAGCTC 1 cut(s) 161
SaqAI TTAA 6 cut(s) 293, 324, 417, 641, 903, 1911
SatI GCNGC 7 cut(s) 348, 521, 591, 688, 974, 1676, 1862
Sau3AI GATC 4 cut(s) 754, 1084, 1279, 1393
Sau96I GGNCC 5 cut(s) 1293, 1477, 1642, 1695, 1795
SchI GAGTC 3 cut(s) 1101, 1733, 1781
ScrFI CCNGG 3 cut(s) 147, 444, 1364
SduI GDGCHC 2 cut(s) 161, 1770
SfaNI GCATC 5 cut(s) 307, 1193, 1411, 1742, 1850
SfcI CTRYAG 2 cut(s) 1008, 1484
SinI GGWCC 4 cut(s) 1293, 1477, 1642, 1795
SmiMI CAYNNNNRTG 1 cut(s) 1737
SmlI CTYRAG 3 cut(s) 640, 947, 1285
SmoI CTYRAG 3 cut(s) 640, 947, 1285
SsiI CCGC 3 cut(s) 660, 736, 1315
SspI AATATT 1 cut(s) 1467
SspMI CTAG 2 cut(s) 746, 1418
SstI GAGCTC 1 cut(s) 161
StyD4I CCNGG 3 cut(s) 145, 442, 1362
StyI CCWWGG 2 cut(s) 1042, 1471
TaaI ACNGT 3 cut(s) 381, 713, 1060
TaiI ACGT 1 cut(s) 417
TaqI TCGA 3 cut(s) 155, 1278, 1942
TaqII GACCGA 1 cut(s) 1812
TatI WGTACW 1 cut(s) 992
TfiI GAWTC 3 cut(s) 1183, 1367, 1712
Tru1I TTAA 6 cut(s) 293, 324, 417, 641, 903, 1911
Tru9I TTAA 6 cut(s) 293, 324, 417, 641, 903, 1911
TscAI CASTG 6 cut(s) 78, 210, 386, 629, 1063, 1548
TseFI GTSAC 4 cut(s) 95, 365, 1070, 1527
TseI GCWGC 7 cut(s) 347, 520, 590, 687, 973, 1675, 1861
Tsp45I GTSAC 4 cut(s) 95, 365, 1070, 1527
TspDTI ATGAA 6 cut(s) 957, 1164, 1445, 1667, 1704, 1755
TspGWI ACGGA 1 cut(s) 296
TspRI CASTG 6 cut(s) 78, 210, 386, 629, 1063, 1548
Van91I CCANNNNNTGG 1 cut(s) 530
Vha464I CTTAAG 1 cut(s) 640
VpaK11BI GGWCC 4 cut(s) 1293, 1477, 1642, 1795
XapI RAATTY 4 cut(s) 174, 1139, 1440, 1654
XbaI TCTAGA 1 cut(s) 1417
XcmI CCANNNNNNNNNTGG 1 cut(s) 1049
XmiI GTMKAC 2 cut(s) 439, 1210
XmnI GAANNNNTTC 3 cut(s) 813, 1555, 1885
XspI CTAG 2 cut(s) 746, 1418
Zsp2I ATGCAT 1 cut(s) 1757
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.