Rw2G027480

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000112
Physical Location & Seq
Reverse (-)
43169279 .. 43172707
3429 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G027480.1

Sequence Viewer

Length: 2472 bp
ATGTCACTGTGTCGCACCCCGAAAGTTGCTAATGACCAGAAAATGTTTTTGAAAGCTCTGCTTCTCTTCCTCCTCTTTCAGTTCTGCACTTCCAGGGACACCATAAAAGGGAATGAAGCACAATTGAAGGATGTTGATGGAGACTATTTAGTGTCCAAAGAAAGCAAGTTTGAATTGGGTTTCTTCAGCCCCGGAAATTCTAGCTACAGGTACGTTGGAATTTGGTATTCTCAGACTAGAGTATCCACTAAAACGGTGGTGTGGGTTGCAAACCGGAACAATCCCATCAATGATACCTCTGGTGTGCTCACAATAAACAGATATGGAGAACTAGTCCTTTATGCTTATAACATGGAGAGCACTCCTATTTGGTCTACTAATGTGTCGCGGTCGGTTCAAAATGCTAACACGAGCACTTTATCTGCACAGCTTTTAGATACAGGAAATTTAGTTGTGTTCCAGGCTGATAATAATGAAAGCTATGTATGGCAAAGTTTTGATTATCCTACTGATACTTTAATTCCAGGTATGAAAGTTGGGGTGAATTGGAGAACCGGGCTGGAATGGGTTTTATCATCTTGGAAGTCACAAGATGACCCTGGAACTGGGGACTATACCTTAAGGCTCTATCCATATCAGATCGCATCCCCCCAATTTTTTATGTACAAGGGTTTAAGTAAGTATTACCGAACTCATCCAGGGCCAACGCCTGGTTTGGTCACTAGTCAGGAGGAAACTTATTATTTCATGAATGAAACCAATGCAATTACAAGAGTAACAGTGACTAATTCTGTGTTGGAGAACCTTCATTGGGATGATGATGGCCTTCAATGGAAGGTAGACTTATCTGCACCGAAGTCCCGGTGTGACGGGTATGGAAAGTGTGGTGCCAACAGCAGATGTAGCCCTGACAACGTTAGTGTGTTTGAGTGTGCATGTTTATCAGGGTATGTGCCTAATTCTATAAGTGATTGGAATCAGAGAAATGGGTCAGGTGGATGTGTGAGTAATCGACTTGGTTTGTTGAAGTGTGGAGATGGAGACGGGTTTGTGAAGGTGGCAAGAGTTAAATATCCAGACACATCGATAGCAGCACGGTTAAAATCAGGAATGAGTGCCAAAGAGTGTGAGCAGGAGTGCCTAAGAAATTGTTCTTGCAAAGCATATTTGAGCACTGAAAATGAAGGGATTGTTGATTGCTTGACATGGTATGATGACTTGATGGACGTTTTAACGTACACAGAGTTTGGACAAGATCTCTACGTTCGTGTGAATGCAACCGTGTTAGCTGCAAATGCCGGAAGATCACAAGGTTTCTTGGAAAGGAGGGGTATGCCGGCCATTCCAATACTGTCTGCTGTGCTGGCATTGGTACTAATCATTATGCTTCTCTATTGGTGGCGTAAGAAGAACAGGAACACAAAAGATTTTGTGGAGGCAGAGGAGCTTGAGGAAGCTCAGAGACACCCCGAATTGCAATTTTTCGATCTTGACACAATAATAGCAGCCACAGACCACTTCTCTCATGTCAATGAACTAGGCCATGGTGGTTTTGGCTCTGTTTTTAAGGGTCAGTTACCAAATGGACAGCAGATTGCTGTGAAAAGATTGTCCAAAACTTCAGGACAAGGGATGGAGGAATTCAAAAATGAAGTTGCACTTATAGCAAGACTTCAACACAGGAATCTTGTGAAACTTTTAGGCTGTTGTATAAAGGGAGAAGAAAGGATGTTAGTCCTAGAATACATGCCTAACAAAAGCTTGGACTCCTTTCTGTTTGATAACAGAAGACGATCCTTCTTGGATTGGAAAAAGCGATTTGAAATTATCAACGGGATTGCTCGTGGGATTTTGTATCTTCACCAAGACTCAAGATTGAGGATTATCCATAGAGACCTAAAAACTAGCAATGTTCTACTAGATGATGAGATGAACCCAAAAGTTTCTGATTTTGGCATGGCTAGAATATTCCATGGGGACAAACTGCAAGATAAGACGAACCGAATTGTCGGAACATATGGCTACATGTCACCAGAGTACGCAGTATTTGGCAGATTTTCCACAAAATCTGATGTCTTTAGTTTTGGGATCATAATGTTGGAGATTGTAAGTGGGCGAAAAAACAATAGTTATGATCTGGAGGATCCTTCCGTGAACTTAATAGGACATGTTTGGGAGCTCTGGAGAGAAGGTAGAGCATTAGATATTGTTGATTCGACACTGAAGTCATGTCAGCCTAATGAAGTCATGAAATGCATACAAGTTGCGCTCTTGTGTGTACAAGAAGATTCAAAGGACCGACCTGCCATGTCAGCCATTGTTATCATGTTGAGTGGTGAAGCATCTCCTCCATTGCCTAAGCAGCCAGCATTTGTTTACAGAAGAGATTCTGGCGCTGATGTTGATCCATTACTTCCAAACGGATCTTATTCTATAAACGACTTGACAATAACTACAATGGAAGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

823

Amino Acids

92.82

Weight (kDa)

6.1

Isoelectric Point (pI)

34.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 85 - 195 4.7e-36 D-mannose binding lectin
S_locus_glycop PF00954 244 - 320 4.8e-13 S-locus glycoprotein domain
PAN_2 PF08276 344 - 410 3.4e-17 PAN-like domain
Pkinase PF00069 508 - 773 1.9e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 510 - 775 9.2e-51 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 348
AasI GACNNNNNNGTC 1 cut(s) 2224
Acc36I ACCTGC 1 cut(s) 2311
AccB1I GGYRCC 1 cut(s) 887
AccB7I CCANNNNNTGG 1 cut(s) 710
AccI GTMKAC 2 cut(s) 374, 840
AccII CGCG 1 cut(s) 388
AciI CCGC 1 cut(s) 388
AclI AACGTT 1 cut(s) 915
AclWI GGATC 6 cut(s) 1788, 2096, 2138, 2151, 2399, 2431
AcoI YGGCCR 1 cut(s) 1338
AcsI RAATTY 4 cut(s) 196, 219, 445, 1640
AcuI CTGAAG 3 cut(s) 169, 1605, 2243
AfaI GTAC 6 cut(s) 212, 665, 1238, 1374, 2039, 2280
AfiI CCNNNNNNNGG 4 cut(s) 108, 605, 710, 811
AflII CTTAAG 1 cut(s) 619
AflIII ACRYGT 2 cut(s) 2025, 2167
AhlI ACTAGT 2 cut(s) 331, 722
AjnI CCWGG 6 cut(s) 92, 459, 523, 598, 697, 709
Alw21I GWGCWC 5 cut(s) 309, 362, 416, 1175, 2181
Alw26I GTCTC 4 cut(s) 135, 1035, 1456, 1887
AlwI GGATC 6 cut(s) 1788, 2096, 2138, 2151, 2399, 2431
AoxI GGCC 4 cut(s) 701, 823, 1338, 1540
ApeKI GCWGC 4 cut(s) 1091, 1289, 1505, 2362
ApoI RAATTY 4 cut(s) 196, 219, 445, 1640
Asp700I GAANNNNTTC 2 cut(s) 1150, 2386
AspLEI GCGC 2 cut(s) 2269, 2396
AspS9I GGNCC 2 cut(s) 701, 2296
AsuC2I CCSGG 3 cut(s) 192, 556, 862
AsuHPI GGTGA 4 cut(s) 553, 1853, 2022, 2348
AvaII GGWCC 1 cut(s) 2296
BaeI ACNNNNGTAYC 2 cut(s) 225, 258
BamHI GGATCC 1 cut(s) 2143
BanI GGYRCC 1 cut(s) 887
BanII GRGCYC 1 cut(s) 2181
BauI CACGAG 2 cut(s) 409, 1842
BbsI GAAGAC 1 cut(s) 1795
Bbv12I GWGCWC 5 cut(s) 309, 362, 416, 1175, 2181
BbvI GCAGC 4 cut(s) 1103, 1276, 1517, 2374
BccI CCATC 6 cut(s) 131, 293, 815, 1031, 1216, 1627
BciT130I CCWGG 6 cut(s) 94, 461, 525, 600, 699, 711
BciVI GTATCC 1 cut(s) 253
BcnI CCSGG 3 cut(s) 192, 556, 862
BcoDI GTCTC 4 cut(s) 135, 1035, 1456, 1887
BcuI ACTAGT 2 cut(s) 331, 722
BfaI CTAG 9 cut(s) 201, 237, 332, 723, 1538, 1739, 1905, 1919, 1962
BfmI CTRYAG 1 cut(s) 205
BfoI RGCGCY 1 cut(s) 2397
BfrI CTTAAG 1 cut(s) 619
BfuAI ACCTGC 1 cut(s) 2311
BfuI GTATCC 1 cut(s) 253
BglII AGATCT 1 cut(s) 1255
BisI GCNGC 4 cut(s) 1092, 1290, 1506, 2363
BlsI GCNGC 4 cut(s) 1093, 1291, 1507, 2364
Bme1390I CCNGG 9 cut(s) 94, 192, 461, 525, 556, 600, 699, 711, 862
Bme18I GGWCC 1 cut(s) 2296
BmgT120I GGNCC 2 cut(s) 701, 2296
BmiI GGNNCC 2 cut(s) 889, 2145
BmrFI CCNGG 9 cut(s) 94, 192, 461, 525, 556, 600, 699, 711, 862
BmrI ACTGGG 1 cut(s) 615
BmsI GCATC 2 cut(s) 653, 2351
BmuI ACTGGG 1 cut(s) 615
BpiI GAAGAC 1 cut(s) 1795
BpmI CTGGAG 2 cut(s) 2159, 2203
Bpu10I CCTNAGC 1 cut(s) 2358
BpuEI CTTGAG 2 cut(s) 1469, 1855
BpuMI CCSGG 3 cut(s) 192, 556, 862
Bsa29I ATCGAT 1 cut(s) 1085
BsaBI GATNNNNATC 2 cut(s) 975, 2403
BsaI GGTCTC 1 cut(s) 1887
BsaJI CCNNGG 6 cut(s) 93, 190, 598, 698, 1543, 1972
BsaWI WCCGGW 1 cut(s) 273
BsaXI ACNNNNNCTCC 4 cut(s) 318, 348, 2176, 2206
Bsc4I CCNNNNNNNGG 4 cut(s) 108, 605, 710, 811
Bse118I RCCGGY 1 cut(s) 1336
Bse1I ACTGG 1 cut(s) 610
Bse3DI GCAATG 2 cut(s) 1915, 2351
Bse8I GATNNNNATC 2 cut(s) 975, 2403
BseBI CCWGG 6 cut(s) 94, 461, 525, 600, 699, 711
BseCI ATCGAT 1 cut(s) 1085
BseDI CCNNGG 6 cut(s) 93, 190, 598, 698, 1543, 1972
BseGI GGATG 7 cut(s) 136, 644, 694, 820, 1004, 1638, 1734
BseJI GATNNNNATC 2 cut(s) 975, 2403
BseLI CCNNNNNNNGG 4 cut(s) 108, 605, 710, 811
BseMI GCAATG 2 cut(s) 1915, 2351
BseMII CTCAG 2 cut(s) 245, 1472
BseNI ACTGG 1 cut(s) 610
BseRI GAGGAG 3 cut(s) 62, 1457, 2337
BseXI GCAGC 4 cut(s) 1103, 1276, 1517, 2374
BsgI GTGCAG 3 cut(s) 70, 408, 834
Bsh1236I CGCG 1 cut(s) 388
Bsh1285I CGRYCG 1 cut(s) 392
BshFI GGCC 4 cut(s) 703, 825, 1340, 1542
BshNI GGYRCC 1 cut(s) 887
BshVI ATCGAT 1 cut(s) 1085
BsiEI CGRYCG 1 cut(s) 392
BsiHKAI GWGCWC 5 cut(s) 309, 362, 416, 1175, 2181
BsiSI CCGG 6 cut(s) 192, 274, 555, 862, 1299, 1337
BslFI GGGAC 4 cut(s) 110, 623, 844, 1991
BslI CCNNNNNNNGG 4 cut(s) 108, 605, 710, 811
BsmAI GTCTC 4 cut(s) 135, 1035, 1456, 1887
BsmBI CGTCTC 1 cut(s) 1035
BsmFI GGGAC 4 cut(s) 110, 623, 844, 1991
BsmI GAATGC 1 cut(s) 1279
BsnI GGCC 4 cut(s) 703, 825, 1340, 1542
Bso31I GGTCTC 1 cut(s) 1887
Bsp1286I GDGCHC 5 cut(s) 309, 362, 416, 1175, 2181
Bsp1407I TGTACA 2 cut(s) 663, 2278
Bsp19I CCATGG 2 cut(s) 1543, 1972
BspACI CCGC 1 cut(s) 388
BspANI GGCC 4 cut(s) 703, 825, 1340, 1542
BspCNI CTCAG 2 cut(s) 244, 1471
BspDI ATCGAT 1 cut(s) 1085
BspFNI CGCG 1 cut(s) 388
BspHI TCATGA 2 cut(s) 747, 2247
BspLI GGNNCC 2 cut(s) 889, 2145
BspMI ACCTGC 1 cut(s) 2311
BspPI GGATC 6 cut(s) 1788, 2096, 2138, 2151, 2399, 2431
BspT107I GGYRCC 1 cut(s) 887
BspTI CTTAAG 1 cut(s) 619
BspTNI GGTCTC 1 cut(s) 1887
BsrDI GCAATG 2 cut(s) 1915, 2351
BsrFI RCCGGY 1 cut(s) 1336
BsrGI TGTACA 2 cut(s) 663, 2278
BsrI ACTGG 1 cut(s) 610
BssAI RCCGGY 1 cut(s) 1336
BssECI CCNNGG 6 cut(s) 93, 190, 598, 698, 1543, 1972
BssSI CACGAG 2 cut(s) 409, 1842
BssT1I CCWWGG 2 cut(s) 1543, 1972
Bst2BI CACGAG 2 cut(s) 409, 1842
Bst2UI CCWGG 6 cut(s) 94, 461, 525, 600, 699, 711
Bst4CI ACNGT 6 cut(s) 9, 256, 781, 1098, 1282, 1353
Bst6I CTCTTC 2 cut(s) 71, 2377
BstAFI CTTAAG 1 cut(s) 619
BstAUI TGTACA 2 cut(s) 663, 2278
BstC8I GCNNGC 3 cut(s) 1338, 1365, 2367
BstDEI CTNAG 4 cut(s) 231, 1142, 1458, 2358
BstDSI CCRYGG 2 cut(s) 1543, 1972
BstF5I GGATG 7 cut(s) 136, 644, 694, 820, 1004, 1638, 1734
BstFNI CGCG 1 cut(s) 388
BstH2I RGCGCY 1 cut(s) 2397
BstHHI GCGC 2 cut(s) 2269, 2396
BstMAI GTCTC 4 cut(s) 135, 1035, 1456, 1887
BstMCI CGRYCG 1 cut(s) 392
BstMWI GCNNNNNNNGC 6 cut(s) 903, 1295, 1364, 1664, 2312, 2362
BstNI CCWGG 6 cut(s) 94, 461, 525, 600, 699, 711
BstNSI RCATGY 4 cut(s) 939, 1750, 2029, 2171
BstSCI CCNGG 9 cut(s) 92, 190, 459, 523, 554, 598, 697, 709, 860
BstSFI CTRYAG 1 cut(s) 205
BstUI CGCG 1 cut(s) 388
BstV1I GCAGC 4 cut(s) 1103, 1276, 1517, 2374
BstV2I GAAGAC 1 cut(s) 1795
BstX2I RGATCY 3 cut(s) 1255, 2143, 2423
BstYI RGATCY 3 cut(s) 1255, 2143, 2423
Bsu15I ATCGAT 1 cut(s) 1085
BsuI GTATCC 1 cut(s) 253
BsuRI GGCC 4 cut(s) 703, 825, 1340, 1542
BsuTUI ATCGAT 1 cut(s) 1085
BtgI CCRYGG 2 cut(s) 1543, 1972
BtsCI GGATG 7 cut(s) 136, 644, 694, 820, 1004, 1638, 1734
BtsIMutI CAGTG 4 cut(s) 5, 786, 1173, 2219
BveI ACCTGC 1 cut(s) 2311
Cac8I GCNNGC 3 cut(s) 1338, 1365, 2367
CciI TCATGA 2 cut(s) 747, 2247
CfoI GCGC 2 cut(s) 2269, 2396
Cfr10I RCCGGY 1 cut(s) 1336
Cfr13I GGNCC 2 cut(s) 701, 2296
ClaI ATCGAT 1 cut(s) 1085
Csp6I GTAC 6 cut(s) 211, 664, 1237, 1373, 2038, 2279
CviQI GTAC 6 cut(s) 211, 664, 1237, 1373, 2038, 2279
DdeI CTNAG 4 cut(s) 231, 1142, 1458, 2358
DrdI GACNNNNNNGTC 1 cut(s) 2224
DseDI GACNNNNNNGTC 1 cut(s) 2224
EaeI YGGCCR 1 cut(s) 1338
Eam1104I CTCTTC 2 cut(s) 71, 2377
EarI CTCTTC 2 cut(s) 71, 2377
Ecl136II GAGCTC 1 cut(s) 2179
Eco130I CCWWGG 2 cut(s) 1543, 1972
Eco24I GRGCYC 1 cut(s) 2181
Eco31I GGTCTC 1 cut(s) 1887
Eco47I GGWCC 1 cut(s) 2296
Eco53kI GAGCTC 1 cut(s) 2179
Eco57I CTGAAG 3 cut(s) 169, 1605, 2243
EcoICRI GAGCTC 1 cut(s) 2179
EcoRI GAATTC 1 cut(s) 1640
EcoRII CCWGG 6 cut(s) 92, 459, 523, 598, 697, 709
EcoT14I CCWWGG 2 cut(s) 1543, 1972
EcoT22I ATGCAT 1 cut(s) 2258
EcoT38I GRGCYC 1 cut(s) 2181
ErhI CCWWGG 2 cut(s) 1543, 1972
Esp3I CGTCTC 1 cut(s) 1035
FalI AAGNNNNNCTT 6 cut(s) 45, 77, 827, 859, 1644, 1676
FaqI GGGAC 4 cut(s) 110, 623, 844, 1991
FauNDI CATATG 1 cut(s) 2017
FblI GTMKAC 2 cut(s) 374, 840
Fnu4HI GCNGC 4 cut(s) 1092, 1290, 1506, 2363
FokI GGATG 7 cut(s) 143, 631, 681, 827, 1011, 1645, 1741
FriOI GRGCYC 1 cut(s) 2181
Fsp4HI GCNGC 4 cut(s) 1092, 1290, 1506, 2363
FspBI CTAG 9 cut(s) 201, 237, 332, 723, 1538, 1739, 1905, 1919, 1962
GlaI GCGC 2 cut(s) 2268, 2395
GluI GCNGC 4 cut(s) 1092, 1290, 1506, 2363
GsuI CTGGAG 2 cut(s) 2159, 2203
HaeII RGCGCY 1 cut(s) 2397
HaeIII GGCC 4 cut(s) 703, 825, 1340, 1542
HapII CCGG 6 cut(s) 192, 274, 555, 862, 1299, 1337
HhaI GCGC 2 cut(s) 2269, 2396
Hin6I GCGC 2 cut(s) 2267, 2394
HinP1I GCGC 2 cut(s) 2267, 2394
HindIII AAGCTT 1 cut(s) 1759
HinfI GANTC 7 cut(s) 976, 1684, 1766, 1868, 2213, 2288, 2387
HpaII CCGG 6 cut(s) 192, 274, 555, 862, 1299, 1337
HphI GGTGA 4 cut(s) 553, 1853, 2022, 2348
Hpy166II GTNNAC 6 cut(s) 375, 841, 1239, 2155, 2279, 2377
Hpy188I TCNGA 7 cut(s) 234, 639, 981, 1461, 1948, 2012, 2071
Hpy8I GTNNAC 6 cut(s) 375, 841, 1239, 2155, 2279, 2377
HpyAV CCTTC 9 cut(s) 121, 815, 829, 836, 1048, 1178, 1807, 2157, 2183
HpyCH4III ACNGT 6 cut(s) 9, 256, 781, 1098, 1282, 1353
HpyCH4IV ACGT 5 cut(s) 213, 915, 1227, 1235, 1263
HpyF10VI GCNNNNNNNGC 6 cut(s) 903, 1295, 1364, 1664, 2312, 2362
HpyF3I CTNAG 4 cut(s) 231, 1142, 1458, 2358
HpySE526I ACGT 5 cut(s) 213, 915, 1227, 1235, 1263
HspAI GCGC 2 cut(s) 2267, 2394
KroI GCCGGC 1 cut(s) 1336
KroNI GCCGGC 1 cut(s) 1338
LmnI GCTCC 2 cut(s) 1444, 2176
Lsp1109I GCAGC 4 cut(s) 1103, 1276, 1517, 2374
LweI GCATC 2 cut(s) 653, 2351
MaeI CTAG 9 cut(s) 201, 237, 332, 723, 1538, 1739, 1905, 1919, 1962
MaeII ACGT 5 cut(s) 213, 915, 1227, 1235, 1263
MaeIII GTNAC 8 cut(s) 3, 585, 718, 775, 781, 866, 1575, 2028
MboII GAAGA 9 cut(s) 58, 175, 1314, 1420, 1733, 1800, 1850, 2297, 2394
MfeI CAATTG 1 cut(s) 122
MflI RGATCY 3 cut(s) 1255, 2143, 2423
MhlI GDGCHC 5 cut(s) 309, 362, 416, 1175, 2181
MlyI GAGTC 2 cut(s) 1760, 1862
MmeI TCCRAC 4 cut(s) 196, 777, 1990, 2079
Mph1103I ATGCAT 1 cut(s) 2258
MroNI GCCGGC 1 cut(s) 1336
MroXI GAANNNNTTC 2 cut(s) 1150, 2386
MseI TTAA 8 cut(s) 518, 620, 674, 1068, 1100, 1232, 1566, 2159
MslI CAYNNNNRTG 2 cut(s) 813, 1530
MspCI CTTAAG 1 cut(s) 619
MspI CCGG 6 cut(s) 192, 274, 555, 862, 1299, 1337
MspR9I CCNGG 9 cut(s) 94, 192, 461, 525, 556, 600, 699, 711, 862
MunI CAATTG 1 cut(s) 122
Mva1269I GAATGC 1 cut(s) 1279
MvaI CCWGG 6 cut(s) 94, 461, 525, 600, 699, 711
MvnI CGCG 1 cut(s) 388
MwoI GCNNNNNNNGC 6 cut(s) 903, 1295, 1364, 1664, 2312, 2362
NaeI GCCGGC 1 cut(s) 1338
NciI CCSGG 3 cut(s) 192, 556, 862
NcoI CCATGG 2 cut(s) 1543, 1972
NdeI CATATG 1 cut(s) 2017
NgoMIV GCCGGC 1 cut(s) 1336
NlaIV GGNNCC 2 cut(s) 889, 2145
NmuCI GTSAC 6 cut(s) 3, 585, 718, 781, 866, 2028
NsiI ATGCAT 1 cut(s) 2258
NspI RCATGY 4 cut(s) 939, 1750, 2029, 2171
PagI TCATGA 2 cut(s) 747, 2247
PciI ACATGT 2 cut(s) 2025, 2167
PctI GAATGC 1 cut(s) 1279
PdiI GCCGGC 1 cut(s) 1338
PdmI GAANNNNTTC 2 cut(s) 1150, 2386
PfeI GAWTC 5 cut(s) 976, 1684, 2213, 2288, 2387
PflMI CCANNNNNTGG 1 cut(s) 710
PkrI GCNGC 4 cut(s) 1093, 1291, 1507, 2364
PleI GAGTC 2 cut(s) 1760, 1862
PpsI GAGTC 2 cut(s) 1760, 1862
PscI ACATGT 2 cut(s) 2025, 2167
PsiI TTATAA 1 cut(s) 348
Psp124BI GAGCTC 1 cut(s) 2181
Psp1406I AACGTT 1 cut(s) 915
Psp6I CCWGG 6 cut(s) 92, 459, 523, 598, 697, 709
PspGI CCWGG 6 cut(s) 92, 459, 523, 598, 697, 709
PspN4I GGNNCC 2 cut(s) 889, 2145
PspPI GGNCC 2 cut(s) 701, 2296
PsuI RGATCY 3 cut(s) 1255, 2143, 2423
RsaI GTAC 6 cut(s) 212, 665, 1238, 1374, 2039, 2280
RsaNI GTAC 6 cut(s) 211, 664, 1237, 1373, 2038, 2279
RseI CAYNNNNRTG 2 cut(s) 813, 1530
SacI GAGCTC 1 cut(s) 2181
SaqAI TTAA 8 cut(s) 518, 620, 674, 1068, 1100, 1232, 1566, 2159
SatI GCNGC 4 cut(s) 1092, 1290, 1506, 2363
Sau96I GGNCC 2 cut(s) 701, 2296
SchI GAGTC 2 cut(s) 1760, 1862
ScrFI CCNGG 9 cut(s) 94, 192, 461, 525, 556, 600, 699, 711, 862
SduI GDGCHC 5 cut(s) 309, 362, 416, 1175, 2181
SfaNI GCATC 2 cut(s) 653, 2351
SfcI CTRYAG 1 cut(s) 205
SinI GGWCC 1 cut(s) 2296
SmiMI CAYNNNNRTG 2 cut(s) 813, 1530
SmlI CTYRAG 3 cut(s) 619, 1448, 1870
SmoI CTYRAG 3 cut(s) 619, 1448, 1870
SpeI ACTAGT 2 cut(s) 331, 722
SsiI CCGC 1 cut(s) 388
SspI AATATT 1 cut(s) 1968
SspMI CTAG 9 cut(s) 201, 237, 332, 723, 1538, 1739, 1905, 1919, 1962
SstI GAGCTC 1 cut(s) 2181
StyD4I CCNGG 9 cut(s) 92, 190, 459, 523, 554, 598, 697, 709, 860
StyI CCWWGG 2 cut(s) 1543, 1972
TaaI ACNGT 6 cut(s) 9, 256, 781, 1098, 1282, 1353
TaiI ACGT 5 cut(s) 216, 918, 1230, 1238, 1266
TaqI TCGA 5 cut(s) 1012, 1085, 1485, 2216, 2467
TaqII GACCGA 1 cut(s) 2313
TatI WGTACW 2 cut(s) 663, 2278
TfiI GAWTC 5 cut(s) 976, 1684, 2213, 2288, 2387
Tru1I TTAA 8 cut(s) 518, 620, 674, 1068, 1100, 1232, 1566, 2159
Tru9I TTAA 8 cut(s) 518, 620, 674, 1068, 1100, 1232, 1566, 2159
TscAI CASTG 4 cut(s) 12, 786, 1180, 2226
TseFI GTSAC 6 cut(s) 3, 585, 718, 781, 866, 2028
TseI GCWGC 4 cut(s) 1091, 1289, 1505, 2362
Tsp45I GTSAC 6 cut(s) 3, 585, 718, 781, 866, 2028
TspGWI ACGGA 2 cut(s) 2140, 2436
TspRI CASTG 4 cut(s) 12, 786, 1180, 2226
Van91I CCANNNNNTGG 1 cut(s) 710
Vha464I CTTAAG 1 cut(s) 619
VpaK11BI GGWCC 1 cut(s) 2296
XapI RAATTY 4 cut(s) 196, 219, 445, 1640
XceI RCATGY 4 cut(s) 939, 1750, 2029, 2171
XcmI CCANNNNNNNNNTGG 2 cut(s) 253, 1550
XmiI GTMKAC 2 cut(s) 374, 840
XmnI GAANNNNTTC 2 cut(s) 1150, 2386
XspI CTAG 9 cut(s) 201, 237, 332, 723, 1538, 1739, 1905, 1919, 1962
Zsp2I ATGCAT 1 cut(s) 2258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.