RchiOBHm_Chr5g0005171

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000112
Physical Location & Seq
Forward (+)
3352637 .. 3356115
3479 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28640

Sequence Viewer

Length: 2484 bp
ATGTTTTTGAATACTTTTCTTCTGTTCCTTCTCTTCCCTTTTTGCATTTCCATAGACACCATAGGACCAAACCAGTATGTAAACGATGGTAAGACCATAGTGTCTAAAGAAATGAAGTTTGAGCTAGGCTTCTTTAGCCCTGGCAACTCTAGTTATCGGTATGTTGGAATTCGGTATGCTAAAATACCTGAAAAACCAGTGATATGGGTGGCAAACAGGGACAATCCGATTAATGATTCATCTGGTGTCCTCACAAACAGGTATGGAAACTTGGTTCTTTATGCTTATAAGGAAAACTTAACTATTTGGTCCACAAAGGTGTCAGACCAAACTACTACTAGTACTTCAGCTCAAATTTTAGATACAGGAAACTTGATTTTGGTCCAGAGTAACAGTGAAGACTTTTTATGGCAAAGCTTTGATTATCCAACGGATACTATGATTCCAGGAATGAAACTTGGGTTGAATCGTGAGACAGGTCTGGATAGCTTCATAACATCGTGGAGGTCAGAAGATGACCCTGGAACTGGAGATTATTCATATAGGCTCAACCTGACTGGCTCCCCACAATTCATTCTGTATAATGGTTCAACTAGGCATTTTCGAACTGCTATGTGGCCATGGAGCCCATCACCGAGTACAACACCTTATGCATATGACTACACTTTTGTCAATAATCAAGATGTGACATATTTGATTGATTCTTCTAATAAATCCTCTGAAATTTCAAGACTTGTGGTTAACTATTATGGCACACTTCAGCACCTAACATGGAATGATGTTGAGGGTCAATGGAAGGAAAAGTGGGCTGCACCAAAGTTCAGGTGTGACCCATACGGACATTGTGGCGCCTTTAGTGGATGTAGTCTTGACAATGTTCAATCTGAGTGTTCTTGTTTGCCAGGGTATCAACCAAGTTCTTCAGAGAATTGGTCTGAGAAAATTTGGTCAGATGGGTGTGTGAGTAAGCAGGTAGAAGTACCTAATAAGTGTGGAAAAAGTTTCGTGAGGGTGGAACATGTGAAGCTTCCAGATGCATCAATATCAGCTTTGCTGGACACAAATATGGGTGAAAGTGAGTGCAAGAAGGCATGCCAGAATAATTGTTCATGCGTCGCATATTCAAGTCTAGAAATTGATGGAGGTGGTGGTTGCATGGCATGGTATGGGGAATTGGTGGACACTTTAATACTGGCAGAGCAGGGACGAGATTTCTATGTTCGAGCAGATGCAACTGAGTCAGCTGAGAATGACAGAAAATCAAGAGGTTTCCTCAGAAGGAGGGGAATGCTTGCTGTTCTAATTATACCTGTTCTGCTGGCACTTTCTCTGATCATGTTGGCCTATTGCTGGTTTATCAAGAGGAAGACTGAAGCCGGTAGATTGAGAAGAACATGGGGTAGACAATACTCTCATTTTAGTACTTACAATGAGTCTATGGTGGAGAATAAAGTAGGGCAAACTAGTAGACACCCAGATTTGCAACTTTTCCATCTCAGCACAATAATAGCTGCAACAAATGACTTCTCTCTGGCTAATAAACTCGGAGAAGGTGGTTTTGGCTCTGTTTATAAGGGCCAGTTATCCAACCAACAGAAGATTGCTGTGAAAAGATTGTCCAAGAGTTCAATACAAGGGGTTGAAGAATTTAAAAACGAAGTTACACTCATTGCAAAACTTCAACACAGGAATCTTGTGAAGCTATTAGGTTGTTGTATAGAGGAAGAAGAGAAGTTGTTAATCTATGAATACTTGCCAAACAAAAGTTTGGACTTCTTTCTTTTTGATCAAAGTCAAAGGTCGGCCTTGGATTGGAGAAACCGATTTGGCATAATTACTGGAGTTGCTCGTGGAATTTTGTATCTCCACGAAGACTCAAGGTTGAGAATTGTCCACAGGGATCTGAAACCCAGCAATGTCCTGCTTGATGCTGACATGAAGCCGAAAATTTCGGATTTCGGCTTGGCCAGAATATTTGATGGGGACCAACTGCAGGATGAGACAAAAAAAATTGTAGGGACATATGGTTATATGTCACCAGAGTATGCAGCATATGGAAAATTTTCAACAAAATCAGATGTGTTTAGCTTTGGGGTCATACTGTTGGAGATTATATGTGGCAAGAAAAACAATGCTTCTTATCAGGACGATCATTCCCTCAATTTGATAGGACATGTATGGGACCTATGGAGAGAACAGTGTGTCCTAGACGTTGTAGATTTCCTACTGGAGTCATATAACATTGATGAAGTCCTGAGATGCCTTCAAATTGGGCTTTTATGCCTGCAAGAAGATACAACAGATAGGCCAACTATGTCCGAAGTTGTCCTAATGTTGAGTGGTGAAAGAGCTCTTGCATCCCCTCAAAGACCGGCATTCATCTTCAAAAACACTTGCAATTGCCACAATAGTTCCGGCTTATCAATAACAGGAGGATCTTATTCTGCTAATGATCTGACAGTCACTAAAGTCGTAGGACGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

827

Amino Acids

93.27

Weight (kDa)

5.92

Isoelectric Point (pI)

41.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 67 - 169 7.1e-35 D-mannose binding lectin
S_locus_glycop PF00954 217 - 306 6.3e-18 S-locus glycoprotein domain
PAN_2 PF08276 332 - 395 2.8e-17 PAN-like domain
PK_Tyr_Ser-Thr PF07714 511 - 776 3.8e-49 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 511 - 776 2.1e-47 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 288, 1572
AasI GACNNNNNNGTC 1 cut(s) 100
Acc36I ACCTGC 1 cut(s) 961
AccB1I GGYRCC 1 cut(s) 848
AccI GTMKAC 2 cut(s) 1402, 1468
AclWI GGATC 2 cut(s) 1908, 2443
AcoI YGGCCR 2 cut(s) 617, 1965
AcsI RAATTY 8 cut(s) 168, 354, 723, 942, 1646, 1854, 1947, 2060
AcuI CTGAAG 4 cut(s) 330, 743, 906, 1392
AcyI GRCGYC 1 cut(s) 849
AfaI GTAC 4 cut(s) 343, 640, 981, 1423
AfiI CCNNNNNNNGG 4 cut(s) 527, 1350, 1812, 1993
AflIII ACRYGT 2 cut(s) 1018, 2173
AhlI ACTAGT 2 cut(s) 338, 1463
AjnI CCWGG 4 cut(s) 139, 445, 520, 901
AjuI GAANNNNNNNTTGG 2 cut(s) 1542, 1574
AleI CACNNNNGTG 1 cut(s) 317
AloI GAACNNNNNNTCC 4 cut(s) 258, 290, 2187, 2219
Alw21I GWGCWC 1 cut(s) 2353
Alw26I GTCTC 2 cut(s) 467, 1994
AlwI GGATC 2 cut(s) 1908, 2443
AoxI GGCC 6 cut(s) 617, 1341, 1576, 1803, 1965, 2306
ApeKI GCWGC 3 cut(s) 809, 1511, 2048
ApoI RAATTY 8 cut(s) 168, 354, 723, 942, 1646, 1854, 1947, 2060
ArsI GACNNNNNNTTYG 2 cut(s) 650, 682
AseI ATTAAT 1 cut(s) 231
Asp700I GAANNNNTTC 1 cut(s) 2062
AspLEI GCGC 1 cut(s) 851
AspS9I GGNCC 6 cut(s) 65, 309, 382, 1576, 1984, 2182
AsuHPI GGTGA 4 cut(s) 624, 1082, 2028, 2354
AsuII TTCGAA 1 cut(s) 604
AvaII GGWCC 5 cut(s) 65, 309, 382, 1984, 2182
BalI TGGCCA 2 cut(s) 619, 1967
BanI GGYRCC 1 cut(s) 848
BanII GRGCYC 2 cut(s) 629, 2353
BauI CACGAG 1 cut(s) 1848
BbsI GAAGAC 3 cut(s) 405, 1373, 1878
Bbv12I GWGCWC 1 cut(s) 2353
BbvI GCAGC 3 cut(s) 796, 1498, 2060
BccI CCATC 6 cut(s) 80, 637, 947, 1133, 1500, 1973
BciT130I CCWGG 4 cut(s) 141, 447, 522, 903
BciVI GTATCC 1 cut(s) 427
BclI TGATCA 2 cut(s) 1332, 1786
BcoDI GTCTC 2 cut(s) 467, 1994
BcuI ACTAGT 2 cut(s) 338, 1463
BfaI CTAG 7 cut(s) 125, 150, 339, 594, 1130, 1464, 2207
BfmI CTRYAG 1 cut(s) 1991
BfoI RGCGCY 1 cut(s) 852
BfuAI ACCTGC 1 cut(s) 961
BfuI GTATCC 1 cut(s) 427
BisI GCNGC 3 cut(s) 810, 1512, 2049
BlsI GCNGC 3 cut(s) 811, 1513, 2050
BmcAI AGTACT 2 cut(s) 343, 1423
Bme1390I CCNGG 4 cut(s) 141, 447, 522, 903
Bme18I GGWCC 5 cut(s) 65, 309, 382, 1984, 2182
BmgT120I GGNCC 6 cut(s) 65, 309, 382, 1576, 1984, 2182
BmiI GGNNCC 5 cut(s) 562, 626, 850, 1985, 2183
BmrFI CCNGG 4 cut(s) 141, 447, 522, 903
BmsI GCATC 6 cut(s) 1024, 1046, 1219, 1918, 2249, 2366
BpiI GAAGAC 3 cut(s) 405, 1373, 1878
BplI GAGNNNNNCTC 2 cut(s) 1257, 1289
BpmI CTGGAG 3 cut(s) 549, 1860, 2249
Bpu14I TTCGAA 1 cut(s) 604
BpuEI CTTGAG 1 cut(s) 1861
BsaHI GRCGYC 1 cut(s) 849
BsaJI CCNNGG 5 cut(s) 139, 520, 620, 902, 1806
Bsc4I CCNNNNNNNGG 4 cut(s) 527, 1350, 1812, 1993
Bse118I RCCGGY 2 cut(s) 1376, 2371
Bse1I ACTGG 8 cut(s) 73, 197, 532, 562, 1197, 1579, 1843, 2232
Bse3DI GCAATG 2 cut(s) 1668, 1921
BseBI CCWGG 4 cut(s) 141, 447, 522, 903
BseDI CCNNGG 5 cut(s) 139, 520, 620, 902, 1806
BseGI GGATG 3 cut(s) 866, 2002, 2357
BseLI CCNNNNNNNGG 4 cut(s) 527, 1350, 1812, 1993
BseMI GCAATG 2 cut(s) 1668, 1921
BseMII CTCAG 7 cut(s) 876, 927, 1227, 1236, 1288, 1510, 2246
BseNI ACTGG 8 cut(s) 73, 197, 532, 562, 1197, 1579, 1843, 2232
BseXI GCAGC 3 cut(s) 796, 1498, 2060
BseYI CCCAGC 1 cut(s) 1910
BsgI GTGCAG 1 cut(s) 795
BshFI GGCC 6 cut(s) 619, 1343, 1578, 1805, 1967, 2308
BshNI GGYRCC 1 cut(s) 848
BsiHKAI GWGCWC 1 cut(s) 2353
BsiSI CCGG 3 cut(s) 1377, 2372, 2415
BslFI GGGAC 5 cut(s) 233, 1218, 1997, 2032, 2195
BslI CCNNNNNNNGG 4 cut(s) 527, 1350, 1812, 1993
BsmAI GTCTC 2 cut(s) 467, 1994
BsmFI GGGAC 5 cut(s) 233, 1218, 1997, 2032, 2195
BsmI GAATGC 2 cut(s) 1293, 2375
BsnI GGCC 6 cut(s) 619, 1343, 1578, 1805, 1967, 2308
Bsp119I TTCGAA 1 cut(s) 604
Bsp1286I GDGCHC 2 cut(s) 629, 2353
Bsp143I GATC 6 cut(s) 1332, 1786, 1900, 2149, 2435, 2452
Bsp19I CCATGG 1 cut(s) 620
BspANI GGCC 6 cut(s) 619, 1343, 1578, 1805, 1967, 2308
BspCNI CTCAG 7 cut(s) 877, 928, 1228, 1237, 1287, 1509, 2247
BspLI GGNNCC 5 cut(s) 562, 626, 850, 1985, 2183
BspMAI CTGCAG 1 cut(s) 1995
BspMI ACCTGC 1 cut(s) 961
BspPI GGATC 2 cut(s) 1908, 2443
BspT104I TTCGAA 1 cut(s) 604
BspT107I GGYRCC 1 cut(s) 848
BsrDI GCAATG 2 cut(s) 1668, 1921
BsrFI RCCGGY 2 cut(s) 1376, 2371
BsrI ACTGG 8 cut(s) 73, 197, 532, 562, 1197, 1579, 1843, 2232
BssAI RCCGGY 2 cut(s) 1376, 2371
BssECI CCNNGG 5 cut(s) 139, 520, 620, 902, 1806
BssMI GATC 6 cut(s) 1332, 1786, 1900, 2149, 2435, 2452
BssNI GRCGYC 1 cut(s) 849
BssSI CACGAG 1 cut(s) 1848
BssT1I CCWWGG 2 cut(s) 620, 1806
Bst2BI CACGAG 1 cut(s) 1848
Bst2UI CCWGG 4 cut(s) 141, 447, 522, 903
Bst4CI ACNGT 4 cut(s) 395, 2103, 2199, 2461
Bst6I CTCTTC 2 cut(s) 38, 1722
BstACI GRCGYC 1 cut(s) 849
BstBI TTCGAA 1 cut(s) 604
BstC8I GCNNGC 4 cut(s) 1093, 1293, 1320, 2285
BstDEI CTNAG 7 cut(s) 885, 936, 1236, 1245, 1274, 1496, 2255
BstDSI CCRYGG 1 cut(s) 620
BstF5I GGATG 3 cut(s) 866, 2002, 2357
BstH2I RGCGCY 1 cut(s) 852
BstHHI GCGC 1 cut(s) 851
BstKTI GATC 6 cut(s) 1335, 1789, 1903, 2152, 2438, 2455
BstMAI GTCTC 2 cut(s) 467, 1994
BstMBI GATC 6 cut(s) 1332, 1786, 1900, 2149, 2435, 2452
BstMWI GCNNNNNNNGC 1 cut(s) 135
BstNI CCWGG 4 cut(s) 141, 447, 522, 903
BstNSI RCATGY 3 cut(s) 1022, 1095, 2177
BstSCI CCNGG 4 cut(s) 139, 445, 520, 901
BstSFI CTRYAG 1 cut(s) 1991
BstV1I GCAGC 3 cut(s) 796, 1498, 2060
BstV2I GAAGAC 3 cut(s) 405, 1373, 1878
BstX2I RGATCY 2 cut(s) 1900, 2435
BstXI CCANNNNNNTGG 1 cut(s) 204
BstYI RGATCY 2 cut(s) 1900, 2435
BsuI GTATCC 1 cut(s) 427
BsuRI GGCC 6 cut(s) 619, 1343, 1578, 1805, 1967, 2308
BtgI CCRYGG 1 cut(s) 620
BtsCI GGATG 3 cut(s) 866, 2002, 2357
BtsIMutI CAGTG 3 cut(s) 204, 400, 2204
BveI ACCTGC 1 cut(s) 961
Cac8I GCNNGC 4 cut(s) 1093, 1293, 1320, 2285
CfoI GCGC 1 cut(s) 851
Cfr10I RCCGGY 2 cut(s) 1376, 2371
Cfr13I GGNCC 6 cut(s) 65, 309, 382, 1576, 1984, 2182
CseI GACGC 1 cut(s) 1102
Csp6I GTAC 4 cut(s) 342, 639, 980, 1422
CspCI CAANNNNNGTGG 2 cut(s) 717, 752
CviQI GTAC 4 cut(s) 342, 639, 980, 1422
DdeI CTNAG 7 cut(s) 885, 936, 1236, 1245, 1274, 1496, 2255
DinI GGCGCC 1 cut(s) 850
DpnI GATC 6 cut(s) 1334, 1788, 1902, 2151, 2437, 2454
DpnII GATC 6 cut(s) 1332, 1786, 1900, 2149, 2435, 2452
DraI TTTAAA 1 cut(s) 1651
DrdI GACNNNNNNGTC 1 cut(s) 100
DseDI GACNNNNNNGTC 1 cut(s) 100
EaeI YGGCCR 2 cut(s) 617, 1965
Eam1104I CTCTTC 2 cut(s) 38, 1722
EarI CTCTTC 2 cut(s) 38, 1722
Ecl136II GAGCTC 1 cut(s) 2351
Eco130I CCWWGG 2 cut(s) 620, 1806
Eco24I GRGCYC 2 cut(s) 629, 2353
Eco47I GGWCC 5 cut(s) 65, 309, 382, 1984, 2182
Eco53kI GAGCTC 1 cut(s) 2351
Eco57I CTGAAG 4 cut(s) 330, 743, 906, 1392
EcoICRI GAGCTC 1 cut(s) 2351
EcoO109I RGGNCCY 1 cut(s) 2182
EcoRI GAATTC 1 cut(s) 168
EcoRII CCWGG 4 cut(s) 139, 445, 520, 901
EcoT14I CCWWGG 2 cut(s) 620, 1806
EcoT22I ATGCAT 2 cut(s) 655, 1039
EcoT38I GRGCYC 2 cut(s) 629, 2353
EgeI GGCGCC 1 cut(s) 850
EheI GGCGCC 1 cut(s) 850
ErhI CCWWGG 2 cut(s) 620, 1806
FalI AAGNNNNNCTT 2 cut(s) 281, 313
FaqI GGGAC 5 cut(s) 233, 1218, 1997, 2032, 2195
FauNDI CATATG 3 cut(s) 655, 2023, 2053
FbaI TGATCA 2 cut(s) 1332, 1786
FblI GTMKAC 2 cut(s) 1402, 1468
Fnu4HI GCNGC 3 cut(s) 810, 1512, 2049
FokI GGATG 3 cut(s) 873, 2009, 2344
FriOI GRGCYC 2 cut(s) 629, 2353
Fsp4HI GCNGC 3 cut(s) 810, 1512, 2049
FspBI CTAG 7 cut(s) 125, 150, 339, 594, 1130, 1464, 2207
GlaI GCGC 1 cut(s) 850
GluI GCNGC 3 cut(s) 810, 1512, 2049
GsaI CCCAGC 1 cut(s) 1914
GsuI CTGGAG 3 cut(s) 549, 1860, 2249
HaeII RGCGCY 1 cut(s) 852
HaeIII GGCC 6 cut(s) 619, 1343, 1578, 1805, 1967, 2308
HapII CCGG 3 cut(s) 1377, 2372, 2415
HgaI GACGC 1 cut(s) 1102
HhaI GCGC 1 cut(s) 851
Hin1I GRCGYC 1 cut(s) 849
Hin6I GCGC 1 cut(s) 849
HinP1I GCGC 1 cut(s) 849
HincII GTYRAC 1 cut(s) 742
HindII GTYRAC 1 cut(s) 742
HindIII AAGCTT 2 cut(s) 415, 1025
HinfI GANTC 9 cut(s) 236, 442, 466, 701, 1238, 1433, 1690, 1874, 2231
HpaI GTTAAC 1 cut(s) 742
HpaII CCGG 3 cut(s) 1377, 2372, 2415
HphI GGTGA 4 cut(s) 624, 1082, 2028, 2354
Hpy166II GTNNAC 7 cut(s) 82, 312, 742, 1180, 1403, 1469, 1894
Hpy8I GTNNAC 7 cut(s) 82, 312, 742, 1180, 1403, 1469, 1894
Hpy99I CGWCG 1 cut(s) 1118
HpyAV CCTTC 6 cut(s) 38, 790, 1081, 1272, 1544, 2273
HpyCH4III ACNGT 4 cut(s) 395, 2103, 2199, 2461
HpyCH4IV ACGT 1 cut(s) 2211
HpyF10VI GCNNNNNNNGC 1 cut(s) 135
HpyF3I CTNAG 7 cut(s) 885, 936, 1236, 1245, 1274, 1496, 2255
HpySE526I ACGT 1 cut(s) 2211
Hsp92I GRCGYC 1 cut(s) 849
HspAI GCGC 1 cut(s) 849
KasI GGCGCC 1 cut(s) 848
Ksp22I TGATCA 2 cut(s) 1332, 1786
KspAI GTTAAC 1 cut(s) 742
Kzo9I GATC 6 cut(s) 1332, 1786, 1900, 2149, 2435, 2452
LmnI GCTCC 2 cut(s) 566, 624
Lsp1109I GCAGC 3 cut(s) 796, 1498, 2060
LweI GCATC 6 cut(s) 1024, 1046, 1219, 1918, 2249, 2366
MaeI CTAG 7 cut(s) 125, 150, 339, 594, 1130, 1464, 2207
MaeII ACGT 1 cut(s) 2211
MaeIII GTNAC 6 cut(s) 389, 685, 827, 1660, 2034, 2461
MalI GATC 6 cut(s) 1334, 1788, 1902, 2151, 2437, 2454
MboI GATC 6 cut(s) 1332, 1786, 1900, 2149, 2435, 2452
MfeI CAATTG 1 cut(s) 2398
MflI RGATCY 2 cut(s) 1900, 2435
MhlI GDGCHC 2 cut(s) 629, 2353
MlsI TGGCCA 2 cut(s) 619, 1967
MluNI TGGCCA 2 cut(s) 619, 1967
Mly113I GGCGCC 1 cut(s) 849
MlyI GAGTC 4 cut(s) 1247, 1442, 1868, 2240
MmeI TCCRAC 4 cut(s) 145, 452, 1611, 2085
Mox20I TGGCCA 2 cut(s) 619, 1967
Mph1103I ATGCAT 2 cut(s) 655, 1039
MroXI GAANNNNTTC 1 cut(s) 2062
MscI TGGCCA 2 cut(s) 619, 1967
MseI TTAA 6 cut(s) 231, 299, 741, 1187, 1650, 1739
MslI CAYNNNNRTG 2 cut(s) 317, 1064
Msp20I TGGCCA 2 cut(s) 619, 1967
MspA1I CMGCKG 1 cut(s) 1244
MspI CCGG 3 cut(s) 1377, 2372, 2415
MspR9I CCNGG 4 cut(s) 141, 447, 522, 903
MunI CAATTG 1 cut(s) 2398
Mva1269I GAATGC 2 cut(s) 1293, 2375
MvaI CCWGG 4 cut(s) 141, 447, 522, 903
MwoI GCNNNNNNNGC 1 cut(s) 135
NarI GGCGCC 1 cut(s) 849
NcoI CCATGG 1 cut(s) 620
NdeI CATATG 3 cut(s) 655, 2023, 2053
NdeII GATC 6 cut(s) 1332, 1786, 1900, 2149, 2435, 2452
NlaIV GGNNCC 5 cut(s) 562, 626, 850, 1985, 2183
NmuCI GTSAC 4 cut(s) 685, 827, 2034, 2461
NsiI ATGCAT 2 cut(s) 655, 1039
NspI RCATGY 3 cut(s) 1022, 1095, 2177
NspV TTCGAA 1 cut(s) 604
OliI CACNNNNGTG 1 cut(s) 317
PaeI GCATGC 1 cut(s) 1095
PciI ACATGT 2 cut(s) 1018, 2173
PctI GAATGC 2 cut(s) 1293, 2375
PdmI GAANNNNTTC 1 cut(s) 2062
PfeI GAWTC 5 cut(s) 236, 442, 466, 701, 1690
PfoI TCCNGGA 1 cut(s) 445
PkrI GCNGC 3 cut(s) 811, 1513, 2050
PleI GAGTC 4 cut(s) 1246, 1441, 1868, 2239
PluTI GGCGCC 1 cut(s) 852
PpsI GAGTC 4 cut(s) 1246, 1441, 1868, 2239
PpuMI RGGWCCY 1 cut(s) 2182
PscI ACATGT 2 cut(s) 1018, 2173
PshBI ATTAAT 1 cut(s) 231
PsiI TTATAA 2 cut(s) 288, 1572
Psp124BI GAGCTC 1 cut(s) 2353
Psp5II RGGWCCY 1 cut(s) 2182
Psp6I CCWGG 4 cut(s) 139, 445, 520, 901
PspFI CCCAGC 1 cut(s) 1910
PspGI CCWGG 4 cut(s) 139, 445, 520, 901
PspN4I GGNNCC 5 cut(s) 562, 626, 850, 1985, 2183
PspPI GGNCC 6 cut(s) 65, 309, 382, 1576, 1984, 2182
PspPPI RGGWCCY 1 cut(s) 2182
PstI CTGCAG 1 cut(s) 1995
PsuI RGATCY 2 cut(s) 1900, 2435
PvuII CAGCTG 1 cut(s) 1244
RsaI GTAC 4 cut(s) 343, 640, 981, 1423
RsaNI GTAC 4 cut(s) 342, 639, 980, 1422
RseI CAYNNNNRTG 2 cut(s) 317, 1064
SacI GAGCTC 1 cut(s) 2353
SaqAI TTAA 6 cut(s) 231, 299, 741, 1187, 1650, 1739
SatI GCNGC 3 cut(s) 810, 1512, 2049
Sau3AI GATC 6 cut(s) 1332, 1786, 1900, 2149, 2435, 2452
Sau96I GGNCC 6 cut(s) 65, 309, 382, 1576, 1984, 2182
ScaI AGTACT 2 cut(s) 343, 1423
SchI GAGTC 4 cut(s) 1247, 1442, 1868, 2240
ScrFI CCNGG 4 cut(s) 141, 447, 522, 903
SduI GDGCHC 2 cut(s) 629, 2353
SfaNI GCATC 6 cut(s) 1024, 1046, 1219, 1918, 2249, 2366
SfcI CTRYAG 1 cut(s) 1991
SfoI GGCGCC 1 cut(s) 850
SfuI TTCGAA 1 cut(s) 604
SinI GGWCC 5 cut(s) 65, 309, 382, 1984, 2182
SmiMI CAYNNNNRTG 2 cut(s) 317, 1064
SmlI CTYRAG 1 cut(s) 1876
SmoI CTYRAG 1 cut(s) 1876
SpeI ACTAGT 2 cut(s) 338, 1463
SphI GCATGC 1 cut(s) 1095
SspDI GGCGCC 1 cut(s) 848
SspI AATATT 1 cut(s) 1974
SspMI CTAG 7 cut(s) 125, 150, 339, 594, 1130, 1464, 2207
SstI GAGCTC 1 cut(s) 2353
StyD4I CCNGG 4 cut(s) 139, 445, 520, 901
StyI CCWWGG 2 cut(s) 620, 1806
TaaI ACNGT 4 cut(s) 395, 2103, 2199, 2461
TaiI ACGT 1 cut(s) 2214
TaqI TCGA 2 cut(s) 604, 1222
TatI WGTACW 3 cut(s) 341, 638, 1421
TfiI GAWTC 5 cut(s) 236, 442, 466, 701, 1690
Tru1I TTAA 6 cut(s) 231, 299, 741, 1187, 1650, 1739
Tru9I TTAA 6 cut(s) 231, 299, 741, 1187, 1650, 1739
TscAI CASTG 3 cut(s) 204, 400, 2204
TseFI GTSAC 4 cut(s) 685, 827, 2034, 2461
TseI GCWGC 3 cut(s) 809, 1511, 2048
Tsp45I GTSAC 4 cut(s) 685, 827, 2034, 2461
TspGWI ACGGA 2 cut(s) 446, 852
TspRI CASTG 3 cut(s) 204, 400, 2204
VpaK11BI GGWCC 5 cut(s) 65, 309, 382, 1984, 2182
VspI ATTAAT 1 cut(s) 231
XapI RAATTY 8 cut(s) 168, 354, 723, 942, 1646, 1854, 1947, 2060
XbaI TCTAGA 1 cut(s) 1129
XceI RCATGY 3 cut(s) 1022, 1095, 2177
XmiI GTMKAC 2 cut(s) 1402, 1468
XmnI GAANNNNTTC 1 cut(s) 2062
XspI CTAG 7 cut(s) 125, 150, 339, 594, 1130, 1464, 2207
ZrmI AGTACT 2 cut(s) 343, 1423
Zsp2I ATGCAT 2 cut(s) 655, 1039
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.