FvH4_3g03410

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000112
Physical Location & Seq
Forward (+)
1842526 .. 1847095
4570 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g03410.t6

Sequence Viewer

Length: 2463 bp
ATGGTGCTGAAGAGTTTGCTACTATTCCTCCTCTTCCCATTTTGCAAAGCTATAGACACCATAGGATCAAATCAGTATGTCAAGGATGGGGACACCCTAATATCTACAGAAAAGAAGTTTGAGTTAGGCTTCTTCAGCCCTGGCAACTCCAGTTACCGGTATGTTGGAATATGGTATGCTGAAATACCTGAAAACCCGGTAGTATGGGTGGCAAACAGAGACAATCCCATCAACGATTCTTCTGGTGTCCTCACAATAAATAGGTATGGAAGTTTGGTTATGTATGCTCACAATAGGGAAGACTTGGTTATATGGTCTACAAAGGCGTTAGTCCAAACCACAAGTAGCACTACTTCAGCTAGGCTTTTGGATACAGGTAACTTGGTTTTGTTCCAGGGTAACAATGATGGAGATGTCTTATGGCAAAGTTTTGATTATCCTACAGATACTATGCTTCCGGGAATGAAACTTGGTTTAAACCGTGTGGTCAAGCTGGACAGCTTTTTAACATCCTGGAAGTCACAATTCGACCCTGGAACTGGGGATTTTTCATATAGCCTCAACCCTACTGGCTCCCCACAATTTTTTATGTACAATGGTTCAACTCCAATGTGGAGAAGTTCTCCGTGGCCATGGCAAGATGCTTCATTAACTCCAAATTATGTAGATATCTACACTATGGTTAATAATCCTAATGAGACATACATGATCGTCACTTCTGATAGCACATCTGGTCTTTCAAGGCTTGTGGTAAACTATTCCGGGACACTCCAGCAATTAACTTGGAATAATGCTGACAGTCGATGGGAGGAAATCTGGGTTGGACCGAAGTACAAATGTGACCGATATGGAGATTGTGGTACTTTTAGTACATGTATTCCTGACAATATTCAATTTGAGTGTGCATGTTTACCAGGGTATCAACCAAGGTCTTTGGAGCATTGGAATCAGAGAAATGGATCGGATGGATGTGTGAGTAATAAACCAGTAGAAGTAGAACGATCACAATGTAAGAATGGAGAAGCATTTAGGAAGGTGGATGGTGTGAAGCTTCCTGATGCATCAATATCAGCTTGGTTTGACACAAATTTGAGTGAAAAGGACTGCAGAAGTACATGCCAAAAGAACTGTTCATGTGTTGCATATTCAAGTGTAGATTATGATGGAGGAGTTGGTTGCTTGATATGGTATGGTGATCTGATGGACACCTTACTGTTATCATATGGGGGACGTGACTTATACATTCGAGGGGATACACTTGAATCAGGAAAATCAAGAGGTTTCATGAGAAGGAGGGGAATGCTTCCTATTCTAATTTTGGCTGTTTCAATTTCACTGCTTCTCATTATGTTTGCCTATTGGTGGCTTTTAAAAAGGCATACGACGAAAGCCAGGGGAAAGTGCTCTCTTTTTAGTTCTTACAATGAGTCTATGGTGGGGAATAAACTAGAAGAAACTAGGAGACATTCAGATTTGCAAATATTTGATCTGAGCACAGTAATAGCTGCAACAGAAGACTTCTCCCATGCTAACAAAATCGGAGAAGGTGGTTTTGGCTCCGTTTATAAGGGCAAGTTGGCCAATGAACAGAAAATTGCTGTGAAAAGATTGTCCAGGTGTTCAAGTCAAGGGATTGAAGAATTCAAAAATGAAGTTATGCTCATTGCGAAACTTCAACACAGGAATCTTGTGAAACTGTTAGGCTGTTGTATAGAACAAGAAGAAAAGTTGTTGATCTATGAGTACTTGCCGAACAAGAGTTTAGACTACTTTCTTTTTGATCAAACTAGAAGATCTTCTTTGGATTGGAGAACTCGGTTTGACATAATCATTGGAGTTGCTCGTGGGATACTATACCTCCATCAAGACTCGAGGCTAAGAATTATCCACAGAGACCTAAAACCTAGCAATGTTCTTCTAGATGCTGACATGAAGCCAAAAATTTCAGATTTTGGCATGGCCAGAATATTTAATGGGGACCTACTACATGATAAGACTAACAGAGTTGTTGGGACATTTGGATATATGTCACCAGAATATGCAGTGTATGGAAAGTTTTCAACAAAATCAGATGTGTTTAGTTTCGGGGTCATTTTGTTGGAGATTGTAAGTGGGAAGAAGAACAATGCCTCTTATCAGGAAGAGCATTCCCTCAATTTGATAGGACATGTTTGGGACCTATGGAAAGAAAAGCGAGTCTTAGACATTGTGGATCCTGAATTAGAGTCGTATGAAATTGATGAAGTCCTTAGATGCATCCAAATCGGTCTCTTATGCCTGCAAGAAGATGTCATGGATCGGCCAACTATGTCTGAAGTTGTTCTCATGTTGAGTGGTGAAAGAGCTCTCGCATCCCCTCAACGGCCAGCGTTCGTCTTCAAGTTCAGCATTGGTGATTCATTAGTAGCAGAGGGAGTTTATTGTTCTAATAATCTGACAATCACTAATGTCATAGGTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

821

Amino Acids

92.81

Weight (kDa)

5.91

Isoelectric Point (pI)

38.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 67 - 173 2.9e-36 D-mannose binding lectin
S_locus_glycop PF00954 205 - 310 1.5e-18 S-locus glycoprotein domain
PAN_2 PF08276 337 - 403 3.5e-18 PAN-like domain
Pkinase PF00069 509 - 710 3.7e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 510 - 719 8.5e-48 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1566
AccI GTMKAC 1 cut(s) 317
AclWI GGATC 5 cut(s) 73, 967, 2207, 2220, 2304
AcoI YGGCCR 5 cut(s) 629, 1578, 1959, 2300, 2363
AcsI RAATTY 3 cut(s) 1087, 1640, 1941
AcuI CTGAAG 4 cut(s) 29, 118, 339, 2334
AfaI GTAC 6 cut(s) 593, 833, 862, 871, 1114, 1745
AfiI CCNNNNNNNGG 4 cut(s) 156, 539, 1362, 2361
AflIII ACRYGT 2 cut(s) 872, 2167
AgeI ACCGGT 1 cut(s) 156
AjiI CACGTC 1 cut(s) 1232
AjnI CCWGG 7 cut(s) 139, 393, 512, 532, 913, 1391, 1613
AjuI GAANNNNNNNTTGG 4 cut(s) 804, 836, 1536, 1568
AluBI AGCT 8 cut(s) 50, 359, 493, 501, 1051, 1073, 1505, 2345
AluI AGCT 8 cut(s) 50, 359, 493, 501, 1051, 1073, 1505, 2345
Alw21I GWGCWC 3 cut(s) 1406, 1496, 2347
Alw26I GTCTC 5 cut(s) 213, 692, 1456, 1887, 2273
AlwI GGATC 5 cut(s) 73, 967, 2207, 2220, 2304
Ama87I CYCGRG 1 cut(s) 1870
AoxI GGCC 5 cut(s) 629, 1578, 1959, 2300, 2363
ApeKI GCWGC 1 cut(s) 1505
ApoI RAATTY 3 cut(s) 1087, 1640, 1941
AsiGI ACCGGT 1 cut(s) 156
Asp700I GAANNNNTTC 3 cut(s) 1795, 2056, 2319
AspS9I GGNCC 3 cut(s) 824, 1978, 2176
AsuC2I CCSGG 3 cut(s) 197, 459, 763
AsuHPI GGTGA 4 cut(s) 1205, 2022, 2348, 2405
AvaI CYCGRG 1 cut(s) 1870
AvaII GGWCC 3 cut(s) 824, 1978, 2176
BaeI ACNNNNGTAYC 2 cut(s) 902, 935
BalI TGGCCA 3 cut(s) 631, 1580, 1961
BamHI GGATCC 1 cut(s) 2212
BanII GRGCYC 1 cut(s) 2347
BarI GAAGNNNNNNTAC 2 cut(s) 337, 369
BauI CACGAG 1 cut(s) 1842
BbsI GAAGAC 3 cut(s) 306, 1521, 2368
Bbv12I GWGCWC 3 cut(s) 1406, 1496, 2347
BbvI GCAGC 1 cut(s) 1492
BccI CCATC 9 cut(s) 80, 236, 401, 798, 959, 1034, 1157, 1195, 1869
BceAI ACGGC 1 cut(s) 2378
BcgI CGANNNNNNTGC 2 cut(s) 2245, 2279
BciT130I CCWGG 7 cut(s) 141, 395, 514, 534, 915, 1393, 1615
BciVI GTATCC 3 cut(s) 364, 1246, 1842
BclI TGATCA 1 cut(s) 1780
BcnI CCSGG 3 cut(s) 197, 459, 763
BcoDI GTCTC 5 cut(s) 213, 692, 1456, 1887, 2273
BfaI CTAG 6 cut(s) 360, 1448, 1458, 1788, 1905, 1919
BfmI CTRYAG 4 cut(s) 51, 105, 441, 1105
BfuI GTATCC 3 cut(s) 364, 1246, 1842
BglII AGATCT 1 cut(s) 1793
BisI GCNGC 1 cut(s) 1506
BlsI GCNGC 1 cut(s) 1507
BmcAI AGTACT 1 cut(s) 1745
Bme18I GGWCC 3 cut(s) 824, 1978, 2176
BmeT110I CYCGRG 1 cut(s) 1870
BmgBI CACGTC 1 cut(s) 1232
BmgT120I GGNCC 3 cut(s) 824, 1978, 2176
BmiI GGNNCC 5 cut(s) 574, 1558, 1979, 2177, 2214
BmrI ACTGGG 1 cut(s) 549
BmsI GCATC 7 cut(s) 631, 1048, 1070, 1912, 2243, 2265, 2360
BmuI ACTGGG 1 cut(s) 549
BpiI GAAGAC 3 cut(s) 306, 1521, 2368
BplI GAGNNNNNCTC 2 cut(s) 607, 639
BpmI CTGGAG 2 cut(s) 133, 755
BpuMI CCSGG 3 cut(s) 197, 459, 763
BsaI GGTCTC 2 cut(s) 1887, 2273
BsaJI CCNNGG 8 cut(s) 139, 394, 532, 626, 632, 914, 926, 1392
BsaWI WCCGGW 1 cut(s) 156
BsaXI ACNNNNNCTCC 4 cut(s) 12, 42, 1842, 1872
Bsc4I CCNNNNNNNGG 4 cut(s) 156, 539, 1362, 2361
Bse118I RCCGGY 1 cut(s) 156
Bse1I ACTGG 4 cut(s) 150, 544, 574, 986
Bse3DI GCAATG 2 cut(s) 1662, 1915
BseBI CCWGG 7 cut(s) 141, 395, 514, 534, 915, 1393, 1615
BseDI CCNNGG 8 cut(s) 139, 394, 532, 626, 632, 914, 926, 1392
BseGI GGATG 7 cut(s) 91, 509, 970, 974, 1045, 2256, 2351
BseLI CCNNNNNNNGG 4 cut(s) 156, 539, 1362, 2361
BseMI GCAATG 2 cut(s) 1662, 1915
BseMII CTCAG 1 cut(s) 1481
BseNI ACTGG 4 cut(s) 150, 544, 574, 986
BseRI GAGGAG 2 cut(s) 20, 1182
BseXI GCAGC 1 cut(s) 1492
BshFI GGCC 5 cut(s) 631, 1580, 1961, 2302, 2365
BshTI ACCGGT 1 cut(s) 156
BsiHKAI GWGCWC 3 cut(s) 1406, 1496, 2347
BsiHKCI CYCGRG 1 cut(s) 1870
BsiSI CCGG 4 cut(s) 157, 197, 458, 762
BslFI GGGAC 6 cut(s) 104, 778, 1242, 1991, 2026, 2189
BslI CCNNNNNNNGG 4 cut(s) 156, 539, 1362, 2361
BsmAI GTCTC 5 cut(s) 213, 692, 1456, 1887, 2273
BsmFI GGGAC 6 cut(s) 104, 778, 1242, 1991, 2026, 2189
BsmI GAATGC 2 cut(s) 1305, 2146
BsnI GGCC 5 cut(s) 631, 1580, 1961, 2302, 2365
Bso31I GGTCTC 2 cut(s) 1887, 2273
BsoBI CYCGRG 1 cut(s) 1870
Bsp1286I GDGCHC 3 cut(s) 1406, 1496, 2347
Bsp1407I TGTACA 1 cut(s) 591
Bsp19I CCATGG 1 cut(s) 632
BspANI GGCC 5 cut(s) 631, 1580, 1961, 2302, 2365
BspCNI CTCAG 1 cut(s) 1482
BspHI TCATGA 1 cut(s) 1284
BspLI GGNNCC 5 cut(s) 574, 1558, 1979, 2177, 2214
BspMAI CTGCAG 1 cut(s) 1109
BspPI GGATC 5 cut(s) 73, 967, 2207, 2220, 2304
BspQI GCTCTTC 1 cut(s) 2136
BspTNI GGTCTC 2 cut(s) 1887, 2273
BsrDI GCAATG 2 cut(s) 1662, 1915
BsrFI RCCGGY 1 cut(s) 156
BsrGI TGTACA 1 cut(s) 591
BsrI ACTGG 4 cut(s) 150, 544, 574, 986
BssAI RCCGGY 1 cut(s) 156
BssECI CCNNGG 8 cut(s) 139, 394, 532, 626, 632, 914, 926, 1392
BssSI CACGAG 1 cut(s) 1842
BssT1I CCWWGG 2 cut(s) 632, 926
Bst2BI CACGAG 1 cut(s) 1842
Bst2UI CCWGG 7 cut(s) 141, 395, 514, 534, 915, 1393, 1615
Bst4CI ACNGT 6 cut(s) 482, 800, 1130, 1215, 1498, 1698
Bst6I CTCTTC 3 cut(s) 5, 38, 2136
BstAUI TGTACA 1 cut(s) 591
BstC8I GCNNGC 2 cut(s) 2279, 2367
BstDEI CTNAG 4 cut(s) 1490, 1877, 2200, 2249
BstDSI CCRYGG 2 cut(s) 626, 632
BstF5I GGATG 7 cut(s) 91, 509, 970, 974, 1045, 2256, 2351
BstMAI GTCTC 5 cut(s) 213, 692, 1456, 1887, 2273
BstMWI GCNNNNNNNGC 1 cut(s) 135
BstNI CCWGG 7 cut(s) 141, 395, 514, 534, 915, 1393, 1615
BstNSI RCATGY 4 cut(s) 876, 909, 1119, 2171
BstSFI CTRYAG 4 cut(s) 51, 105, 441, 1105
BstV1I GCAGC 1 cut(s) 1492
BstV2I GAAGAC 3 cut(s) 306, 1521, 2368
BstX2I RGATCY 2 cut(s) 1793, 2212
BstYI RGATCY 2 cut(s) 1793, 2212
BsuI GTATCC 3 cut(s) 364, 1246, 1842
BsuRI GGCC 5 cut(s) 631, 1580, 1961, 2302, 2365
BtgI CCRYGG 2 cut(s) 626, 632
BtrI CACGTC 1 cut(s) 1232
BtsCI GGATG 7 cut(s) 91, 509, 970, 974, 1045, 2256, 2351
BtsI GCAGTG 2 cut(s) 1334, 2049
BtsIMutI CAGTG 2 cut(s) 1334, 2049
Cac8I GCNNGC 2 cut(s) 2279, 2367
CciI TCATGA 1 cut(s) 1284
Cfr10I RCCGGY 1 cut(s) 156
Cfr13I GGNCC 3 cut(s) 824, 1978, 2176
Csp6I GTAC 6 cut(s) 592, 832, 861, 870, 1113, 1744
CspAI ACCGGT 1 cut(s) 156
CspCI CAANNNNNGTGG 2 cut(s) 729, 764
CviQI GTAC 6 cut(s) 592, 832, 861, 870, 1113, 1744
DdeI CTNAG 4 cut(s) 1490, 1877, 2200, 2249
DraI TTTAAA 2 cut(s) 477, 1371
EaeI YGGCCR 5 cut(s) 629, 1578, 1959, 2300, 2363
Eam1104I CTCTTC 3 cut(s) 5, 38, 2136
EarI CTCTTC 3 cut(s) 5, 38, 2136
Ecl136II GAGCTC 1 cut(s) 2345
Eco130I CCWWGG 2 cut(s) 632, 926
Eco24I GRGCYC 1 cut(s) 2347
Eco31I GGTCTC 2 cut(s) 1887, 2273
Eco32I GATATC 1 cut(s) 670
Eco47I GGWCC 3 cut(s) 824, 1978, 2176
Eco53kI GAGCTC 1 cut(s) 2345
Eco57I CTGAAG 4 cut(s) 29, 118, 339, 2334
Eco88I CYCGRG 1 cut(s) 1870
EcoICRI GAGCTC 1 cut(s) 2345
EcoO109I RGGNCCY 2 cut(s) 1978, 2176
EcoRI GAATTC 1 cut(s) 1640
EcoRII CCWGG 7 cut(s) 139, 393, 512, 532, 913, 1391, 1613
EcoRV GATATC 1 cut(s) 670
EcoT14I CCWWGG 2 cut(s) 632, 926
EcoT22I ATGCAT 2 cut(s) 1063, 2258
EcoT38I GRGCYC 1 cut(s) 2347
ErhI CCWWGG 2 cut(s) 632, 926
FalI AAGNNNNNCTT 4 cut(s) 1783, 1815, 2183, 2215
FaqI GGGAC 6 cut(s) 104, 778, 1242, 1991, 2026, 2189
FauNDI CATATG 1 cut(s) 1222
FbaI TGATCA 1 cut(s) 1780
FblI GTMKAC 1 cut(s) 317
Fnu4HI GCNGC 1 cut(s) 1506
FokI GGATG 7 cut(s) 98, 496, 977, 981, 1052, 2243, 2338
FriOI GRGCYC 1 cut(s) 2347
Fsp4HI GCNGC 1 cut(s) 1506
FspBI CTAG 6 cut(s) 360, 1448, 1458, 1788, 1905, 1919
GluI GCNGC 1 cut(s) 1506
GsuI CTGGAG 2 cut(s) 133, 755
HaeIII GGCC 5 cut(s) 631, 1580, 1961, 2302, 2365
HapII CCGG 4 cut(s) 157, 197, 458, 762
HindIII AAGCTT 1 cut(s) 1049
HinfI GANTC 9 cut(s) 236, 946, 1262, 1427, 1684, 1868, 2196, 2225, 2396
HpaII CCGG 4 cut(s) 157, 197, 458, 762
HphI GGTGA 4 cut(s) 1205, 2022, 2348, 2405
Hpy166II GTNNAC 3 cut(s) 318, 754, 911
Hpy188III TCNNGA 9 cut(s) 881, 1055, 1266, 1275, 1285, 1865, 1919, 2138, 2216
Hpy8I GTNNAC 3 cut(s) 318, 754, 911
Hpy99I CGWCG 1 cut(s) 1387
HpyAV CCTTC 3 cut(s) 1027, 1284, 1538
HpyCH4III ACNGT 6 cut(s) 482, 800, 1130, 1215, 1498, 1698
HpyCH4IV ACGT 1 cut(s) 1231
HpyF10VI GCNNNNNNNGC 1 cut(s) 135
HpyF3I CTNAG 4 cut(s) 1490, 1877, 2200, 2249
HpySE526I ACGT 1 cut(s) 1231
Ksp22I TGATCA 1 cut(s) 1780
LguI GCTCTTC 1 cut(s) 2136
LmnI GCTCC 3 cut(s) 578, 937, 1562
Lsp1109I GCAGC 1 cut(s) 1492
LweI GCATC 7 cut(s) 631, 1048, 1070, 1912, 2243, 2265, 2360
MaeI CTAG 6 cut(s) 360, 1448, 1458, 1788, 1905, 1919
MaeII ACGT 1 cut(s) 1231
MaeIII GTNAC 8 cut(s) 152, 377, 398, 519, 712, 839, 1232, 2028
MflI RGATCY 2 cut(s) 1793, 2212
MhlI GDGCHC 3 cut(s) 1406, 1496, 2347
MlsI TGGCCA 3 cut(s) 631, 1580, 1961
MluNI TGGCCA 3 cut(s) 631, 1580, 1961
MlyI GAGTC 4 cut(s) 1436, 1862, 2205, 2234
MmeI TCCRAC 3 cut(s) 145, 802, 2079
Mox20I TGGCCA 3 cut(s) 631, 1580, 1961
Mph1103I ATGCAT 2 cut(s) 1063, 2258
MroXI GAANNNNTTC 3 cut(s) 1795, 2056, 2319
MscI TGGCCA 3 cut(s) 631, 1580, 1961
MseI TTAA 7 cut(s) 476, 506, 650, 684, 779, 1370, 1971
Msp20I TGGCCA 3 cut(s) 631, 1580, 1961
MspI CCGG 4 cut(s) 157, 197, 458, 762
MssI GTTTAAAC 1 cut(s) 477
Mva1269I GAATGC 2 cut(s) 1305, 2146
MvaI CCWGG 7 cut(s) 141, 395, 514, 534, 915, 1393, 1615
MwoI GCNNNNNNNGC 1 cut(s) 135
NciI CCSGG 3 cut(s) 197, 459, 763
NcoI CCATGG 1 cut(s) 632
NdeI CATATG 1 cut(s) 1222
NlaIV GGNNCC 5 cut(s) 574, 1558, 1979, 2177, 2214
NmuCI GTSAC 5 cut(s) 519, 712, 839, 1232, 2028
NsiI ATGCAT 2 cut(s) 1063, 2258
NspI RCATGY 4 cut(s) 876, 909, 1119, 2171
PaeR7I CTCGAG 1 cut(s) 1870
PagI TCATGA 1 cut(s) 1284
PciI ACATGT 2 cut(s) 872, 2167
PciSI GCTCTTC 1 cut(s) 2136
PctI GAATGC 2 cut(s) 1305, 2146
PdmI GAANNNNTTC 3 cut(s) 1795, 2056, 2319
PfeI GAWTC 5 cut(s) 236, 946, 1262, 1684, 2396
PfoI TCCNGGA 3 cut(s) 457, 512, 761
PinAI ACCGGT 1 cut(s) 156
PkrI GCNGC 1 cut(s) 1507
PleI GAGTC 4 cut(s) 1435, 1862, 2204, 2233
PmeI GTTTAAAC 1 cut(s) 477
PpsI GAGTC 4 cut(s) 1435, 1862, 2204, 2233
PpuMI RGGWCCY 2 cut(s) 1978, 2176
PscI ACATGT 2 cut(s) 872, 2167
PsiI TTATAA 1 cut(s) 1566
Psp124BI GAGCTC 1 cut(s) 2347
Psp5II RGGWCCY 2 cut(s) 1978, 2176
Psp6I CCWGG 7 cut(s) 139, 393, 512, 532, 913, 1391, 1613
PspGI CCWGG 7 cut(s) 139, 393, 512, 532, 913, 1391, 1613
PspN4I GGNNCC 5 cut(s) 574, 1558, 1979, 2177, 2214
PspPI GGNCC 3 cut(s) 824, 1978, 2176
PspPPI RGGWCCY 2 cut(s) 1978, 2176
PspXI VCTCGAGB 1 cut(s) 1870
PstI CTGCAG 1 cut(s) 1109
PsuI RGATCY 2 cut(s) 1793, 2212
RsaI GTAC 6 cut(s) 593, 833, 862, 871, 1114, 1745
RsaNI GTAC 6 cut(s) 592, 832, 861, 870, 1113, 1744
SacI GAGCTC 1 cut(s) 2347
SapI GCTCTTC 1 cut(s) 2136
SaqAI TTAA 7 cut(s) 476, 506, 650, 684, 779, 1370, 1971
SatI GCNGC 1 cut(s) 1506
Sau96I GGNCC 3 cut(s) 824, 1978, 2176
ScaI AGTACT 1 cut(s) 1745
SchI GAGTC 4 cut(s) 1436, 1862, 2205, 2234
SduI GDGCHC 3 cut(s) 1406, 1496, 2347
SfaNI GCATC 7 cut(s) 631, 1048, 1070, 1912, 2243, 2265, 2360
SfcI CTRYAG 4 cut(s) 51, 105, 441, 1105
Sfr274I CTCGAG 1 cut(s) 1870
SinI GGWCC 3 cut(s) 824, 1978, 2176
SlaI CTCGAG 1 cut(s) 1870
SmlI CTYRAG 1 cut(s) 1870
SmoI CTYRAG 1 cut(s) 1870
SspI AATATT 3 cut(s) 889, 1482, 1968
SspMI CTAG 6 cut(s) 360, 1448, 1458, 1788, 1905, 1919
SstI GAGCTC 1 cut(s) 2347
StyI CCWWGG 2 cut(s) 632, 926
TaaI ACNGT 6 cut(s) 482, 800, 1130, 1215, 1498, 1698
TaiI ACGT 1 cut(s) 1234
TaqI TCGA 5 cut(s) 528, 802, 1246, 1871, 2458
TaqII GACCGA 3 cut(s) 841, 858, 2255
TatI WGTACW 5 cut(s) 591, 831, 869, 1112, 1743
TfiI GAWTC 5 cut(s) 236, 946, 1262, 1684, 2396
Tru1I TTAA 7 cut(s) 476, 506, 650, 684, 779, 1370, 1971
Tru9I TTAA 7 cut(s) 476, 506, 650, 684, 779, 1370, 1971
TscAI CASTG 2 cut(s) 1341, 2049
TseFI GTSAC 5 cut(s) 519, 712, 839, 1232, 2028
TseI GCWGC 1 cut(s) 1505
Tsp45I GTSAC 5 cut(s) 519, 712, 839, 1232, 2028
TspGWI ACGGA 2 cut(s) 615, 1549
TspRI CASTG 2 cut(s) 1341, 2049
VpaK11BI GGWCC 3 cut(s) 824, 1978, 2176
XapI RAATTY 3 cut(s) 1087, 1640, 1941
XbaI TCTAGA 1 cut(s) 1918
XceI RCATGY 4 cut(s) 876, 909, 1119, 2171
XhoI CTCGAG 1 cut(s) 1870
XmiI GTMKAC 1 cut(s) 317
XmnI GAANNNNTTC 3 cut(s) 1795, 2056, 2319
XspI CTAG 6 cut(s) 360, 1448, 1458, 1788, 1905, 1919
ZrmI AGTACT 1 cut(s) 1745
Zsp2I ATGCAT 2 cut(s) 1063, 2258
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.