RchiOBHm_Chr7g0224061

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000112
Physical Location & Seq
Forward (+)
45922584 .. 45925835
3252 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20062

Sequence Viewer

Length: 2481 bp
ATGCCACTGTTTCACATCTGGAAAGTTGCTAACCCCCAGAAAATGGTTTTGAAAGCTCTGCTTCTCTTCCTCCTCTTTCAGGTCTGCACTTCCAGGGATACCATAAAAGGGAATGAAGAACAATTGAAGGATGTTGATGGAGACTATTTAGTGTCCAAAGAAAGCAAGTTTGACTTGGGTTTCTTCAGCCCCGGAAATTCTAGCTACCGGTATGTTGGAATCTGGTATTCTCAGACTAGAGTATCTAATAAAACAGTGGTGTGGGTGGCAAACAGGAACAATCCTATCAATGATACCTCTGGTGTGCTCACAATAAACAGGTATGGAGAACTAGTCCTTTATGCTCATAACATGGAGAGCACTCCTATTTGGTCTATTTATGTGTCGCGGTCGGTTCAAAATGTTAACACAAGCACTTCATCTGCACAGCTTTTAGATACAGGAAATTTAGTTGTGTTCCAGGATGATAAAAATGAAATCTTTATATGGCAAAGTTTTGATCATCCTATAGATACTTTAATTCCAGGTATGAAAGTTGGGGTGAATTGGAAAACTGGGCTAGAATGGGTTTTAACATCTTGGAAGTCACAAGATGACCCTGGAACTGGGGACTATACCTATAGGCTAAGTTCAGATCATAATGCATTTCCCCAATTTTTTATGTATAAAGGTTTGAGTAAGTATTGGCGAAGTGATCCAGGCCCAGCGCCTACTTTGGTCACTAATCAAGATGAAACTTATTATTTCATTAATGAAACCAATGCAATTACAAGAATAACAGTGACTGATCCTGCCTTAGAGCGGCTTACGTGGGATAATGGTGGCCTTCAATGGAAGGAAGAATTCTCTGCACCGAAGTCCCGGTGTGACAGGTATGGACAGTGTGGTGCCAACAGCAGATGTAGCCCTGACAATGTTAATCTGTTTGAGTGTGACTGTTTGCCAGGGTATGTGCCTAATTCTATAAGTGATTGGAATCAGAAAAATGGTTCAGGTGGATGTGTGAGTAATCGACTTGGTTTGTTGAATTGTGGAGATGGAGACGGGTTTATAAAGGTGGCAAGAGTTAAATATCCAGACACATCAACAGCAGCATCGTTAAAATCAGGTATGAGCGACAAAGAGTGTGAGCAGGAGTGCCTAAGAAATTGTTCTTGCACTGCATATTTGAGCACTGAAAATGAAGGGATTGTTGATTGCTTGACATGGTATGATGACTTGATGGACATTTTAGTGTACACAAAGCTTGGACAAGATCTCTTCGTTCGTGTGAATGCTACCGTGTTAGCGGCAAATGCCGGAAGATCACAAGGTTTCTTGGAAAGGAGGGGTATGCTGGCTATTCCAATACTGTCTTCTGTGCTTGCATTGGTACTAATCATAATACTTGCATGGAAGTGGCGTAAGAAGAACAGGAACACAAGAGGCATAGACTTTGTGGAGGCAGAGGAGCTAGAGGAAACTCAGAGACACCCTGAATTGCAATTTTTCGATCTTGACACAATAATAGCAGCCACAGACCACTTCTCTCGTGTCAATGAACTTGGCCACGGTGGCTTTGGCTCTGTTTATAAGGGTCAGCTAGCAAATGAACAGAAGGTTGCTGTGAAAAGATTGTCCAAAACTTCAGGACAAGGGATCGAAGAATTTAAGAATGAAGTTGCACTTATAGCAAGACTTCAACACAGGAACCTTGTGAAACTTTTAGGCTGTTGTATAAAGGGAGAAGAAAGGATGTTAGTGCTAGAATACATGCCTAACAAAAGCTTGGACTCCTTTCTTTTTGATAAGACAAGACGGTCCTTCTTGGATTGGGAAAAGCGTTTTGAAATTATCAACGGGATTGCGCGTGGGATACTATATCTTCACCAAGACTCAAGATTGAGGATTATCCATAGAGATCTAAAAACTAGTAATGTTCTACTAGATGATGAGATGAACCCAAAAATTTCTGATTTTGGCATGGCTAGAATATTCCATGGGGACCAACTGCAAGATAAGACGAGCCGAATTGTCGGAACATATGGCTACATGTCACCGGAGTACGCAGTCTTTGGGAGATTTTCCACAAAATCTGATGTCTTTAGTTTTGGGATCATAATGTTGGAGATTGTAAGTGGCCAGAAAAACAGTGGCTCTGATCTGGAGGATCCTTCTGTGAACTTAATAGGACGTGTTTGGGAGCTCTGGAGAGAAGGCAGAGCCTTGGATATTGTGGATTCAACACTGAAGTCATGTCAGCCTAATGAAGTCCTTAGATGCATACAAGTTGCGCTCTTGTGTGTACAAGAAGATTCAAAGGACCGACCTGCCATGTCAGCCATTGTTTTCATGTTGAGTGGTGAAGCATCTCCTTCACTGCCTAAGCAGCCGGCATTTGTTTACAGAAGAAATTTCGGCACTGATGTTGATCCATTACTTTCAAATAGATCTTCTTCTATAAATGACTTGACAGTAACTACAATGGAAGCTCGACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

826

Amino Acids

93.32

Weight (kDa)

5.76

Isoelectric Point (pI)

38.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 85 - 195 4.2e-35 D-mannose binding lectin
S_locus_glycop PF00954 242 - 320 4.5e-13 S-locus glycoprotein domain
PAN_2 PF08276 344 - 410 1.3e-17 PAN-like domain
Pkinase PF00069 510 - 775 1.4e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 512 - 777 6.7e-52 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1052, 1572
AasI GACNNNNNNGTC 1 cut(s) 1798
Acc36I ACCTGC 1 cut(s) 2317
AccB1I GGYRCC 1 cut(s) 887
AccB7I CCANNNNNTGG 1 cut(s) 43
AccBSI CCGCTC 1 cut(s) 802
AccII CGCG 2 cut(s) 388, 1849
AciI CCGC 3 cut(s) 388, 802, 1289
AclWI GGATC 7 cut(s) 689, 782, 1646, 2102, 2144, 2157, 2405
AcoI YGGCCR 2 cut(s) 1546, 2119
AcsI RAATTY 6 cut(s) 196, 445, 842, 1646, 1947, 2392
AcuI CTGAAG 3 cut(s) 169, 1611, 2249
AfaI GTAC 4 cut(s) 1238, 1374, 2045, 2286
AfiI CCNNNNNNNGG 5 cut(s) 43, 79, 108, 605, 801
AflIII ACRYGT 2 cut(s) 2031, 2173
AgeI ACCGGT 1 cut(s) 207
AhlI ACTAGT 2 cut(s) 331, 1910
AjiI CACGTC 1 cut(s) 2174
AjnI CCWGG 6 cut(s) 92, 459, 523, 598, 697, 943
AjuI GAANNNNNNNTTGG 2 cut(s) 1339, 1371
AluBI AGCT 9 cut(s) 56, 204, 430, 1246, 1453, 1582, 1767, 2185, 2471
AluI AGCT 9 cut(s) 56, 204, 430, 1246, 1453, 1582, 1767, 2185, 2471
Alw21I GWGCWC 4 cut(s) 309, 362, 1175, 2187
Alw26I GTCTC 3 cut(s) 135, 1035, 1462
AlwI GGATC 7 cut(s) 689, 782, 1646, 2102, 2144, 2157, 2405
AlwNI CAGNNNCTG 1 cut(s) 785
AoxI GGCC 4 cut(s) 700, 823, 1546, 2119
ApeKI GCWGC 3 cut(s) 1091, 1511, 2368
ApoI RAATTY 6 cut(s) 196, 445, 842, 1646, 1947, 2392
AseI ATTAAT 1 cut(s) 750
AsiGI ACCGGT 1 cut(s) 207
Asp700I GAANNNNTTC 1 cut(s) 1150
AspLEI GCGC 3 cut(s) 709, 1849, 2275
AspS9I GGNCC 4 cut(s) 701, 1800, 1984, 2302
AsuC2I CCSGG 2 cut(s) 192, 862
AsuHPI GGTGA 4 cut(s) 553, 1859, 2028, 2354
AsuNHI GCTAGC 1 cut(s) 1582
AvaII GGWCC 3 cut(s) 1800, 1984, 2302
BaeI ACNNNNGTAYC 2 cut(s) 225, 258
BalI TGGCCA 2 cut(s) 1548, 2121
BamHI GGATCC 1 cut(s) 2149
BanI GGYRCC 1 cut(s) 887
BanII GRGCYC 1 cut(s) 2187
BauI CACGAG 1 cut(s) 1530
BbsI GAAGAC 1 cut(s) 1347
Bbv12I GWGCWC 4 cut(s) 309, 362, 1175, 2187
BbvI GCAGC 3 cut(s) 1103, 1523, 2380
BccI CCATC 3 cut(s) 131, 1031, 1216
BciT130I CCWGG 6 cut(s) 94, 461, 525, 600, 699, 945
BciVI GTATCC 2 cut(s) 91, 1848
BclI TGATCA 1 cut(s) 499
BcnI CCSGG 2 cut(s) 192, 862
BcoDI GTCTC 3 cut(s) 135, 1035, 1462
BcuI ACTAGT 2 cut(s) 331, 1910
BfmI CTRYAG 2 cut(s) 507, 619
BfoI RGCGCY 1 cut(s) 710
BfuAI ACCTGC 1 cut(s) 2317
BfuI GTATCC 2 cut(s) 91, 1848
BglI GCCNNNNNGGC 1 cut(s) 1554
BglII AGATCT 3 cut(s) 1255, 1900, 2429
BisI GCNGC 5 cut(s) 803, 1092, 1290, 1512, 2369
BlsI GCNGC 5 cut(s) 804, 1093, 1291, 1513, 2370
Bme1390I CCNGG 8 cut(s) 94, 192, 461, 525, 600, 699, 862, 945
Bme18I GGWCC 3 cut(s) 1800, 1984, 2302
BmgBI CACGTC 1 cut(s) 2174
BmgT120I GGNCC 4 cut(s) 701, 1800, 1984, 2302
BmiI GGNNCC 4 cut(s) 889, 1691, 1985, 2151
BmrFI CCNGG 8 cut(s) 94, 192, 461, 525, 600, 699, 862, 945
BmrI ACTGGG 2 cut(s) 564, 615
BmsI GCATC 3 cut(s) 1103, 2249, 2357
BmtI GCTAGC 1 cut(s) 1586
BmuI ACTGGG 2 cut(s) 564, 615
BpiI GAAGAC 1 cut(s) 1347
BpmI CTGGAG 2 cut(s) 2165, 2209
Bpu10I CCTNAGC 1 cut(s) 2364
BpuEI CTTGAG 1 cut(s) 1861
BpuMI CCSGG 2 cut(s) 192, 862
BsaAI YACGTR 1 cut(s) 810
BsaBI GATNNNNATC 2 cut(s) 975, 2409
BsaJI CCNNGG 7 cut(s) 93, 190, 598, 944, 1549, 1978, 2205
BsaWI WCCGGW 2 cut(s) 207, 2038
BsaXI ACNNNNNCTCC 2 cut(s) 318, 348
Bsc4I CCNNNNNNNGG 5 cut(s) 43, 79, 108, 605, 801
Bse118I RCCGGY 2 cut(s) 207, 2371
Bse1I ACTGG 2 cut(s) 559, 610
Bse8I GATNNNNATC 2 cut(s) 975, 2409
BseBI CCWGG 6 cut(s) 94, 461, 525, 600, 699, 945
BseDI CCNNGG 7 cut(s) 93, 190, 598, 944, 1549, 1978, 2205
BseGI GGATG 5 cut(s) 136, 469, 502, 1004, 1740
BseJI GATNNNNATC 2 cut(s) 975, 2409
BseLI CCNNNNNNNGG 5 cut(s) 43, 79, 108, 605, 801
BseMII CTCAG 2 cut(s) 245, 1478
BseNI ACTGG 2 cut(s) 559, 610
BseRI GAGGAG 2 cut(s) 62, 1463
BseXI GCAGC 3 cut(s) 1103, 1523, 2380
BseYI CCCAGC 1 cut(s) 703
BsgI GTGCAG 3 cut(s) 70, 408, 834
Bsh1236I CGCG 2 cut(s) 388, 1849
Bsh1285I CGRYCG 1 cut(s) 392
BshFI GGCC 4 cut(s) 702, 825, 1548, 2121
BshNI GGYRCC 1 cut(s) 887
BshTI ACCGGT 1 cut(s) 207
BsiEI CGRYCG 1 cut(s) 392
BsiHKAI GWGCWC 4 cut(s) 309, 362, 1175, 2187
BsiSI CCGG 6 cut(s) 192, 208, 862, 1299, 2039, 2372
BslFI GGGAC 3 cut(s) 623, 844, 1997
BslI CCNNNNNNNGG 5 cut(s) 43, 79, 108, 605, 801
BsmAI GTCTC 3 cut(s) 135, 1035, 1462
BsmBI CGTCTC 1 cut(s) 1035
BsmFI GGGAC 3 cut(s) 623, 844, 1997
BsmI GAATGC 1 cut(s) 1279
BsnI GGCC 4 cut(s) 702, 825, 1548, 2121
Bsp1286I GDGCHC 4 cut(s) 309, 362, 1175, 2187
Bsp1407I TGTACA 2 cut(s) 1236, 2284
Bsp19I CCATGG 1 cut(s) 1978
BspACI CCGC 3 cut(s) 388, 802, 1289
BspANI GGCC 4 cut(s) 702, 825, 1548, 2121
BspCNI CTCAG 2 cut(s) 244, 1477
BspFNI CGCG 2 cut(s) 388, 1849
BspLI GGNNCC 4 cut(s) 889, 1691, 1985, 2151
BspMI ACCTGC 1 cut(s) 2317
BspOI GCTAGC 1 cut(s) 1586
BspPI GGATC 7 cut(s) 689, 782, 1646, 2102, 2144, 2157, 2405
BspT107I GGYRCC 1 cut(s) 887
BsrBI CCGCTC 1 cut(s) 802
BsrFI RCCGGY 2 cut(s) 207, 2371
BsrGI TGTACA 2 cut(s) 1236, 2284
BsrI ACTGG 2 cut(s) 559, 610
BssAI RCCGGY 2 cut(s) 207, 2371
BssECI CCNNGG 7 cut(s) 93, 190, 598, 944, 1549, 1978, 2205
BssSI CACGAG 1 cut(s) 1530
BssT1I CCWWGG 2 cut(s) 1978, 2205
Bst2BI CACGAG 1 cut(s) 1530
Bst2UI CCWGG 6 cut(s) 94, 461, 525, 600, 699, 945
Bst6I CTCTTC 2 cut(s) 71, 1265
BstAUI TGTACA 2 cut(s) 1236, 2284
BstBAI YACGTR 1 cut(s) 810
BstC8I GCNNGC 4 cut(s) 1338, 1365, 1584, 2373
BstDEI CTNAG 7 cut(s) 231, 626, 796, 1142, 1464, 2255, 2364
BstDSI CCRYGG 2 cut(s) 1549, 1978
BstENI CCTNNNNNAGG 1 cut(s) 77
BstF5I GGATG 5 cut(s) 136, 469, 502, 1004, 1740
BstFNI CGCG 2 cut(s) 388, 1849
BstH2I RGCGCY 1 cut(s) 710
BstHHI GCGC 3 cut(s) 709, 1849, 2275
BstMAI GTCTC 3 cut(s) 135, 1035, 1462
BstMCI CGRYCG 1 cut(s) 392
BstMWI GCNNNNNNNGC 6 cut(s) 903, 1295, 1554, 1670, 2318, 2368
BstNI CCWGG 6 cut(s) 94, 461, 525, 600, 699, 945
BstNSI RCATGY 2 cut(s) 1756, 2035
BstSCI CCNGG 8 cut(s) 92, 190, 459, 523, 598, 697, 860, 943
BstSFI CTRYAG 2 cut(s) 507, 619
BstUI CGCG 2 cut(s) 388, 1849
BstV1I GCAGC 3 cut(s) 1103, 1523, 2380
BstV2I GAAGAC 1 cut(s) 1347
BstX2I RGATCY 4 cut(s) 1255, 1900, 2149, 2429
BstYI RGATCY 4 cut(s) 1255, 1900, 2149, 2429
BsuI GTATCC 2 cut(s) 91, 1848
BsuRI GGCC 4 cut(s) 702, 825, 1548, 2121
BtgI CCRYGG 2 cut(s) 1549, 1978
BtrI CACGTC 1 cut(s) 2174
BtsCI GGATG 5 cut(s) 136, 469, 502, 1004, 1740
BtsI GCAGTG 2 cut(s) 1158, 2357
BveI ACCTGC 1 cut(s) 2317
Cac8I GCNNGC 4 cut(s) 1338, 1365, 1584, 2373
CaiI CAGNNNCTG 1 cut(s) 785
CfoI GCGC 3 cut(s) 709, 1849, 2275
Cfr10I RCCGGY 2 cut(s) 207, 2371
Cfr13I GGNCC 4 cut(s) 701, 1800, 1984, 2302
Csp6I GTAC 4 cut(s) 1237, 1373, 2044, 2285
CspAI ACCGGT 1 cut(s) 207
CviQI GTAC 4 cut(s) 1237, 1373, 2044, 2285
DdeI CTNAG 7 cut(s) 231, 626, 796, 1142, 1464, 2255, 2364
DrdI GACNNNNNNGTC 1 cut(s) 1798
DseDI GACNNNNNNGTC 1 cut(s) 1798
EaeI YGGCCR 2 cut(s) 1546, 2119
Eam1104I CTCTTC 2 cut(s) 71, 1265
EarI CTCTTC 2 cut(s) 71, 1265
Ecl136II GAGCTC 1 cut(s) 2185
Eco130I CCWWGG 2 cut(s) 1978, 2205
Eco24I GRGCYC 1 cut(s) 2187
Eco47I GGWCC 3 cut(s) 1800, 1984, 2302
Eco53kI GAGCTC 1 cut(s) 2185
Eco57I CTGAAG 3 cut(s) 169, 1611, 2249
EcoICRI GAGCTC 1 cut(s) 2185
EcoNI CCTNNNNNAGG 1 cut(s) 77
EcoRI GAATTC 1 cut(s) 842
EcoRII CCWGG 6 cut(s) 92, 459, 523, 598, 697, 943
EcoT14I CCWWGG 2 cut(s) 1978, 2205
EcoT22I ATGCAT 2 cut(s) 646, 2264
EcoT38I GRGCYC 1 cut(s) 2187
ErhI CCWWGG 2 cut(s) 1978, 2205
Esp3I CGTCTC 1 cut(s) 1035
FalI AAGNNNNNCTT 6 cut(s) 45, 77, 158, 190, 1650, 1682
FaqI GGGAC 3 cut(s) 623, 844, 1997
FauNDI CATATG 1 cut(s) 2023
FbaI TGATCA 1 cut(s) 499
Fnu4HI GCNGC 5 cut(s) 803, 1092, 1290, 1512, 2369
FokI GGATG 5 cut(s) 143, 476, 489, 1011, 1747
FriOI GRGCYC 1 cut(s) 2187
Fsp4HI GCNGC 5 cut(s) 803, 1092, 1290, 1512, 2369
GlaI GCGC 3 cut(s) 708, 1848, 2274
GluI GCNGC 5 cut(s) 803, 1092, 1290, 1512, 2369
GsaI CCCAGC 1 cut(s) 707
GsuI CTGGAG 2 cut(s) 2165, 2209
HaeII RGCGCY 1 cut(s) 710
HaeIII GGCC 4 cut(s) 702, 825, 1548, 2121
HapII CCGG 6 cut(s) 192, 208, 862, 1299, 2039, 2372
HhaI GCGC 3 cut(s) 709, 1849, 2275
Hin6I GCGC 3 cut(s) 707, 1847, 2273
HinP1I GCGC 3 cut(s) 707, 1847, 2273
HincII GTYRAC 1 cut(s) 406
HindII GTYRAC 1 cut(s) 406
HindIII AAGCTT 2 cut(s) 1244, 1765
HinfI GANTC 6 cut(s) 219, 976, 1772, 1874, 2219, 2294
HpaI GTTAAC 1 cut(s) 406
HpaII CCGG 6 cut(s) 192, 208, 862, 1299, 2039, 2372
HphI GGTGA 4 cut(s) 553, 1859, 2028, 2354
Hpy166II GTNNAC 6 cut(s) 406, 1237, 1239, 2161, 2285, 2383
Hpy188I TCNGA 8 cut(s) 234, 634, 981, 1467, 1954, 2018, 2077, 2140
Hpy188III TCNNGA 8 cut(s) 19, 728, 1076, 1496, 1629, 1878, 2144, 2188
Hpy8I GTNNAC 6 cut(s) 406, 1237, 1239, 2161, 2285, 2383
HpyAV CCTTC 9 cut(s) 121, 829, 836, 1178, 1591, 1813, 2163, 2189, 2364
HpyCH4IV ACGT 2 cut(s) 809, 2173
HpyF10VI GCNNNNNNNGC 6 cut(s) 903, 1295, 1554, 1670, 2318, 2368
HpyF3I CTNAG 7 cut(s) 231, 626, 796, 1142, 1464, 2255, 2364
HpySE526I ACGT 2 cut(s) 809, 2173
HspAI GCGC 3 cut(s) 707, 1847, 2273
KroI GCCGGC 1 cut(s) 2371
KroNI GCCGGC 1 cut(s) 2373
Ksp22I TGATCA 1 cut(s) 499
KspAI GTTAAC 1 cut(s) 406
LmnI GCTCC 2 cut(s) 1450, 2182
Lsp1109I GCAGC 3 cut(s) 1103, 1523, 2380
LweI GCATC 3 cut(s) 1103, 2249, 2357
MaeII ACGT 2 cut(s) 809, 2173
MaeIII GTNAC 7 cut(s) 585, 718, 781, 866, 932, 2034, 2455
MbiI CCGCTC 1 cut(s) 802
MfeI CAATTG 1 cut(s) 122
MflI RGATCY 4 cut(s) 1255, 1900, 2149, 2429
MhlI GDGCHC 4 cut(s) 309, 362, 1175, 2187
MlsI TGGCCA 2 cut(s) 1548, 2121
MluNI TGGCCA 2 cut(s) 1548, 2121
MlyI GAGTC 2 cut(s) 1766, 1868
MmeI TCCRAC 3 cut(s) 196, 1996, 2085
Mox20I TGGCCA 2 cut(s) 1548, 2121
Mph1103I ATGCAT 2 cut(s) 646, 2264
MroNI GCCGGC 1 cut(s) 2371
MroXI GAANNNNTTC 1 cut(s) 1150
MscI TGGCCA 2 cut(s) 1548, 2121
MseI TTAA 9 cut(s) 405, 518, 572, 750, 918, 1068, 1100, 1650, 2165
MslI CAYNNNNRTG 2 cut(s) 1232, 1396
Msp20I TGGCCA 2 cut(s) 1548, 2121
MspI CCGG 6 cut(s) 192, 208, 862, 1299, 2039, 2372
MspR9I CCNGG 8 cut(s) 94, 192, 461, 525, 600, 699, 862, 945
MunI CAATTG 1 cut(s) 122
Mva1269I GAATGC 1 cut(s) 1279
MvaI CCWGG 6 cut(s) 94, 461, 525, 600, 699, 945
MvnI CGCG 2 cut(s) 388, 1849
MwoI GCNNNNNNNGC 6 cut(s) 903, 1295, 1554, 1670, 2318, 2368
NaeI GCCGGC 1 cut(s) 2373
NciI CCSGG 2 cut(s) 192, 862
NcoI CCATGG 1 cut(s) 1978
NdeI CATATG 1 cut(s) 2023
NgoMIV GCCGGC 1 cut(s) 2371
NheI GCTAGC 1 cut(s) 1582
NlaIV GGNNCC 4 cut(s) 889, 1691, 1985, 2151
NmuCI GTSAC 6 cut(s) 585, 718, 781, 866, 932, 2034
NsiI ATGCAT 2 cut(s) 646, 2264
NspI RCATGY 2 cut(s) 1756, 2035
PciI ACATGT 1 cut(s) 2031
PctI GAATGC 1 cut(s) 1279
PdiI GCCGGC 1 cut(s) 2373
PdmI GAANNNNTTC 1 cut(s) 1150
PfeI GAWTC 4 cut(s) 219, 976, 2219, 2294
PflMI CCANNNNNTGG 1 cut(s) 43
PfoI TCCNGGA 1 cut(s) 459
PinAI ACCGGT 1 cut(s) 207
PkrI GCNGC 5 cut(s) 804, 1093, 1291, 1513, 2370
PleI GAGTC 2 cut(s) 1766, 1868
PpsI GAGTC 2 cut(s) 1766, 1868
Ppu21I YACGTR 1 cut(s) 810
PscI ACATGT 1 cut(s) 2031
PshBI ATTAAT 1 cut(s) 750
PsiI TTATAA 2 cut(s) 1052, 1572
Psp124BI GAGCTC 1 cut(s) 2187
Psp6I CCWGG 6 cut(s) 92, 459, 523, 598, 697, 943
PspFI CCCAGC 1 cut(s) 703
PspGI CCWGG 6 cut(s) 92, 459, 523, 598, 697, 943
PspN4I GGNNCC 4 cut(s) 889, 1691, 1985, 2151
PspPI GGNCC 4 cut(s) 701, 1800, 1984, 2302
PstNI CAGNNNCTG 1 cut(s) 785
PsuI RGATCY 4 cut(s) 1255, 1900, 2149, 2429
RsaI GTAC 4 cut(s) 1238, 1374, 2045, 2286
RsaNI GTAC 4 cut(s) 1237, 1373, 2044, 2285
RseI CAYNNNNRTG 2 cut(s) 1232, 1396
SacI GAGCTC 1 cut(s) 2187
SaqAI TTAA 9 cut(s) 405, 518, 572, 750, 918, 1068, 1100, 1650, 2165
SatI GCNGC 5 cut(s) 803, 1092, 1290, 1512, 2369
Sau96I GGNCC 4 cut(s) 701, 1800, 1984, 2302
SchI GAGTC 2 cut(s) 1766, 1868
ScrFI CCNGG 8 cut(s) 94, 192, 461, 525, 600, 699, 862, 945
SduI GDGCHC 4 cut(s) 309, 362, 1175, 2187
SfaNI GCATC 3 cut(s) 1103, 2249, 2357
SfcI CTRYAG 2 cut(s) 507, 619
SinI GGWCC 3 cut(s) 1800, 1984, 2302
SmiMI CAYNNNNRTG 2 cut(s) 1232, 1396
SmlI CTYRAG 1 cut(s) 1876
SmoI CTYRAG 1 cut(s) 1876
SpeI ACTAGT 2 cut(s) 331, 1910
SsiI CCGC 3 cut(s) 388, 802, 1289
SspI AATATT 1 cut(s) 1974
SstI GAGCTC 1 cut(s) 2187
StyD4I CCNGG 8 cut(s) 92, 190, 459, 523, 598, 697, 860, 943
StyI CCWWGG 2 cut(s) 1978, 2205
TaiI ACGT 2 cut(s) 812, 2176
TaqI TCGA 4 cut(s) 1012, 1491, 1641, 2473
TaqII GACCGA 1 cut(s) 2319
TatI WGTACW 2 cut(s) 1236, 2284
TauI GCSGC 2 cut(s) 805, 1292
TfiI GAWTC 4 cut(s) 219, 976, 2219, 2294
Tru1I TTAA 9 cut(s) 405, 518, 572, 750, 918, 1068, 1100, 1650, 2165
Tru9I TTAA 9 cut(s) 405, 518, 572, 750, 918, 1068, 1100, 1650, 2165
TseFI GTSAC 6 cut(s) 585, 718, 781, 866, 932, 2034
TseI GCWGC 3 cut(s) 1091, 1511, 2368
Tsp45I GTSAC 6 cut(s) 585, 718, 781, 866, 932, 2034
Van91I CCANNNNNTGG 1 cut(s) 43
VpaK11BI GGWCC 3 cut(s) 1800, 1984, 2302
VspI ATTAAT 1 cut(s) 750
XagI CCTNNNNNAGG 1 cut(s) 77
XapI RAATTY 6 cut(s) 196, 445, 842, 1646, 1947, 2392
XceI RCATGY 2 cut(s) 1756, 2035
XcmI CCANNNNNNNNNTGG 2 cut(s) 1556, 2129
XmnI GAANNNNTTC 1 cut(s) 1150
Zsp2I ATGCAT 2 cut(s) 646, 2264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.