Rw5G002190

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000112
Physical Location & Seq
Forward (+)
1850827 .. 1854042
3216 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G002190.1

Sequence Viewer

Length: 2436 bp
ATGTTGGTCAAAGCTCTGCTTCTCTTGCTCCTCTTCCGATTTTGCGATTCCAGGGACACCTTAACATGGAACCAAGAACAACTGAAGGATGATGGTGGAGTTTTAGTGTCCAAACAGAGCAAGTTCGAGTTGGGTTTCTTCAGCCCCGGAAATTCTAGCTACCGGTATATTGGAATTTGGTATTCTCAGACTAAAGTATGTGAGAAAACAGTGGTGTGGGTTGCAAACAGGAACAATCCCATCAATGATACCTCTGGTGTGCTCAAAATAAACAGGTATGGAGAACTGGCCCTTTATTCTTATAACATGAACAGCACTCCTGTTTGGTCCACTAATGTGTCACGGCCGGTCCAAAATAATAACGCAAGCACTTTTTCTGCACAGCTTTTAGATGCAGGAAATTTAGTTGTGTTCCAGGATGATAATAATGAAATCTATCCATGGCAAAGTTTTGATTATCCTACAGATACTCTAATTCCAGGTATGAAAGTCGGGGTGAATTGGAAAACTGGGCTACAATGGGTTTTAACATCTTGGAAGTCACGAGATGACCCTGGACTTGGGGACTATACCTTTAGGCTTTATCCAGATCAGACTGCCGCCCCCCAGTATTTTGTGTATAAAGGTTTGAGCAAGTACTGGCGAAGTGAGTCAGGGCCAGCGCCTCCTTTTGTCACTAATCAAGAGGAAACTTATTATTTCTTCAATTACACCAATGCAATTTCAAGAACGACGTTGACCGATTCTGGTAGGTTAAATCGCCTTACATGGGATAATGGTGGCCTTCAATGGAAGGAAGTCTATTCGGCACCGAAGTCCCGGTGTGACTGGTATGGACAGTGTGGTGCCAACAGCAAATGTAAGCCTGACAATATTAATGAGTTTGAGTGTGAATGTTTACCAGGGTATGAGCCTAGTTCTATAAATGATTGGAATCAGAAAAATGGGTCGGGTGGATGTGTGAGTAAGCGAGTTGGTGTATCAAAGTGCGGGAATGGAGATGGGTTTGTGAATGTGGCAAGAGTTAAATTTCCAGACACATCAATAGCATCACGGTTGAGAACAGGTATGAGTGCCAAAGAGTGTGAGCAGGACTGCTTAAGAAATTGTTCTTGCACCGCATATTTGAGCATCGAATATGAAGGGCTTGTGGATTGCTTGACATGGTATGATGACTTGATGGATATTTTAGTGTACACAGAGCGTGGACGAGATTTATACGTACGTGTAAATAAAACAGAGTTAGCTGCATATGCAGGAAGATCACAAGTTTTCTTGGAAAGGAGGGGTATGCTCGCTATTCCAATATTGTCTGCTCTACTGGCATTGGTCCTAATCATTATGCTTGCTTGTTGGTGGAGTAAGAAGAACAGGAACACAAAAGGCATAGTCTTTGTGGAGGCAGAGGAGCTTGAGGAAACTCAGAGACACCCCGAATTGCAATTTTTCGATCTTGACACAATAATAGCAGCCACAGACGACTTCTCTCGTGTCAATGAACTTGGCCATGGTGGTTTTGGCTCTGTTTATAAGGGTCAGCTACCAAATGAACAGAAAGTTGCTGTGAAAAGATTGTCCAAAACTTCAGGACAAGGGATTGAAGAATTCAAAAATGAAGTTGCACTTATAGCAAGACTTCAACACAGGAACCTTGTGAAACTTTTAGGCTGTTGTATAAAGGGAGAAGAAAGGATATTAGTCCTAGAATACATGCCTAACAAAAGCTTGGACTCCTTTCTTTTTGATAACACAAGAAGGTCCTTCTTGGATTGGGAAAAGCGCTTTGAAATTATCAACGGAATTGCTCGTGGTATTCTATATCTTCACCAAGACTCAAGATTGAGGATTATCCATAGAGATCTAAAAACTAGCAATGTTCTACTAGATGCTATGATGAACCCAAAAATTTCTGATTTTGGCATGGCTAGAATATTCCACGGGGACCAACTGCAAGATAAGACGAACCGAATTGTCGGAACATATGGCTACATGTCACCGGAGTACGCGGTATTTGGAAGATTTTCCACAAAATCTGATGTCTTTAGTTTTGGGATCATAATGTTGGAGATTTTAAGTGGCCAGAAAAACAATGGTTTTGATCTAGAAGATCCTTCCATGAACTTAATAGGACGTGTTTGGGAGCTCTGGGAAGAAGGCATAGCCTTAGATATTTTGGATTCGACACTGAAGTCATATCAGCCTAATGAAGTCATGAGATGCATACAAGTTGCGCTCTTGTGTGTACAAGAAGATTCGAAGGACCGACCTGCCATGTCAGCCGTTGTTTTCATGTTGAGTGGTGAATCATCTCGTCCATTGCCTAAGCAGCCGGCATTTGTTTACAGAAGAAATTCTGACACTGATGTTTATCCATTACTTTCAAACAGATCTTATTCTATAAACGATGTGACAGCATCTACAATGGAAGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

811

Amino Acids

92.23

Weight (kDa)

5.95

Isoelectric Point (pI)

41.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 70 - 180 5e-35 D-mannose binding lectin
S_locus_glycop PF00954 223 - 306 7.3e-17 S-locus glycoprotein domain
PAN_2 PF08276 330 - 396 1.6e-17 PAN-like domain
Pkinase PF00069 496 - 759 1.3e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 498 - 762 2e-50 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 772 - 811 4.7e-06 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 303, 1530
AasI GACNNNNNNGTC 1 cut(s) 2188
Acc36I ACCTGC 1 cut(s) 2275
AccB1I GGYRCC 2 cut(s) 808, 845
AccII CGCG 1 cut(s) 2006
AciI CCGC 4 cut(s) 600, 990, 1119, 2006
AclWI GGATC 2 cut(s) 2060, 2102
AcoI YGGCCR 3 cut(s) 344, 1504, 2077
AcsI RAATTY 7 cut(s) 151, 174, 400, 1028, 1604, 1905, 2350
AcuI CTGAAG 4 cut(s) 104, 124, 1569, 2207
AfaI GTAC 5 cut(s) 638, 1196, 1224, 2003, 2244
AfeI AGCGCT 1 cut(s) 1781
AfiI CCNNNNNNNGG 3 cut(s) 66, 560, 769
AflII CTTAAG 1 cut(s) 1099
AflIII ACRYGT 3 cut(s) 1225, 1989, 2131
AgeI ACCGGT 1 cut(s) 162
AgsI TTSAA 8 cut(s) 706, 726, 788, 1601, 1609, 1640, 1787, 2382
AjiI CACGTC 1 cut(s) 2132
AjnI CCWGG 5 cut(s) 50, 414, 478, 553, 901
AleI CACNNNNGTG 1 cut(s) 335
AluBI AGCT 9 cut(s) 14, 159, 385, 1247, 1411, 1540, 1725, 2143, 2429
AluI AGCT 9 cut(s) 14, 159, 385, 1247, 1411, 1540, 1725, 2143, 2429
Alw21I GWGCWC 2 cut(s) 264, 2145
Alw26I GTCTC 1 cut(s) 1420
AlwI GGATC 2 cut(s) 2060, 2102
Aor51HI AGCGCT 1 cut(s) 1781
AoxI GGCC 6 cut(s) 288, 344, 656, 781, 1504, 2077
ApeKI GCWGC 3 cut(s) 1247, 1469, 2326
ApoI RAATTY 7 cut(s) 151, 174, 400, 1028, 1604, 1905, 2350
AseI ATTAAT 1 cut(s) 876
AsiGI ACCGGT 1 cut(s) 162
Asp700I GAANNNNTTC 3 cut(s) 1108, 2020, 2350
AspLEI GCGC 3 cut(s) 664, 1782, 2233
AspS9I GGNCC 8 cut(s) 289, 327, 349, 656, 1330, 1758, 1942, 2260
AsuC2I CCSGG 2 cut(s) 147, 820
AsuHPI GGTGA 4 cut(s) 508, 1817, 1986, 2312
AsuII TTCGAA 1 cut(s) 2255
AvaII GGWCC 6 cut(s) 327, 349, 1330, 1758, 1942, 2260
BalI TGGCCA 2 cut(s) 1506, 2079
BanI GGYRCC 2 cut(s) 808, 845
BanII GRGCYC 1 cut(s) 2145
BauI CACGAG 3 cut(s) 543, 1488, 1806
Bbv12I GWGCWC 2 cut(s) 264, 2145
BbvI GCAGC 3 cut(s) 1234, 1481, 2338
BccI CCATC 4 cut(s) 86, 248, 995, 1174
BceAI ACGGC 2 cut(s) 359, 2264
BciT130I CCWGG 5 cut(s) 52, 416, 480, 555, 903
BcnI CCSGG 2 cut(s) 147, 820
BcoDI GTCTC 1 cut(s) 1420
BfaI CTAG 7 cut(s) 156, 915, 1703, 1869, 1883, 1926, 2102
BfmI CTRYAG 1 cut(s) 462
BfoI RGCGCY 2 cut(s) 665, 1783
BfrI CTTAAG 1 cut(s) 1099
BfuAI ACCTGC 1 cut(s) 2275
BglII AGATCT 2 cut(s) 1858, 2387
BisI GCNGC 4 cut(s) 600, 1248, 1470, 2327
BlsI GCNGC 4 cut(s) 601, 1249, 1471, 2328
BmcAI AGTACT 1 cut(s) 638
Bme1390I CCNGG 7 cut(s) 52, 147, 416, 480, 555, 820, 903
Bme18I GGWCC 6 cut(s) 327, 349, 1330, 1758, 1942, 2260
BmgBI CACGTC 1 cut(s) 2132
BmgT120I GGNCC 8 cut(s) 289, 327, 349, 656, 1330, 1758, 1942, 2260
BmiI GGNNCC 5 cut(s) 71, 810, 847, 1649, 1943
BmrFI CCNGG 7 cut(s) 52, 147, 416, 480, 555, 820, 903
BmrI ACTGGG 2 cut(s) 519, 601
BmsI GCATC 6 cut(s) 382, 1058, 1140, 1876, 2207, 2423
BmuI ACTGGG 2 cut(s) 519, 601
Bpu10I CCTNAGC 1 cut(s) 2322
Bpu14I TTCGAA 1 cut(s) 2255
BpuEI CTTGAG 2 cut(s) 1433, 1819
BpuMI CCSGG 2 cut(s) 147, 820
BsaAI YACGTR 2 cut(s) 1222, 1226
BsaBI GATNNNNATC 2 cut(s) 933, 2367
BsaJI CCNNGG 7 cut(s) 51, 145, 440, 553, 902, 1507, 1936
BsaWI WCCGGW 2 cut(s) 162, 1996
BsaXI ACNNNNNCTCC 2 cut(s) 90, 120
Bsc4I CCNNNNNNNGG 3 cut(s) 66, 560, 769
Bse118I RCCGGY 3 cut(s) 162, 346, 2329
Bse1I ACTGG 6 cut(s) 291, 514, 607, 644, 833, 1326
Bse3DI GCAATG 2 cut(s) 1879, 2315
Bse8I GATNNNNATC 2 cut(s) 933, 2367
BseBI CCWGG 5 cut(s) 52, 416, 480, 555, 903
BseDI CCNNGG 7 cut(s) 51, 145, 440, 553, 902, 1507, 1936
BseGI GGATG 3 cut(s) 94, 424, 962
BseJI GATNNNNATC 2 cut(s) 933, 2367
BseLI CCNNNNNNNGG 3 cut(s) 66, 560, 769
BseMI GCAATG 2 cut(s) 1879, 2315
BseMII CTCAG 2 cut(s) 200, 1436
BseNI ACTGG 6 cut(s) 291, 514, 607, 644, 833, 1326
BseRI GAGGAG 2 cut(s) 20, 1421
BseX3I CGGCCG 1 cut(s) 344
BseXI GCAGC 3 cut(s) 1234, 1481, 2338
BsgI GTGCAG 1 cut(s) 363
Bsh1236I CGCG 1 cut(s) 2006
Bsh1285I CGRYCG 1 cut(s) 347
BshFI GGCC 6 cut(s) 290, 346, 658, 783, 1506, 2079
BshNI GGYRCC 2 cut(s) 808, 845
BshTI ACCGGT 1 cut(s) 162
BsiEI CGRYCG 1 cut(s) 347
BsiHKAI GWGCWC 2 cut(s) 264, 2145
BsiSI CCGG 6 cut(s) 147, 163, 347, 820, 1997, 2330
BsiWI CGTACG 1 cut(s) 1222
BslFI GGGAC 4 cut(s) 68, 578, 802, 1955
BslI CCNNNNNNNGG 3 cut(s) 66, 560, 769
BsmAI GTCTC 1 cut(s) 1420
BsmFI GGGAC 4 cut(s) 68, 578, 802, 1955
BsnI GGCC 6 cut(s) 290, 346, 658, 783, 1506, 2079
Bsp119I TTCGAA 1 cut(s) 2255
Bsp1286I GDGCHC 2 cut(s) 264, 2145
Bsp1407I TGTACA 2 cut(s) 1194, 2242
Bsp143I GATC 8 cut(s) 589, 1262, 1450, 1858, 2052, 2098, 2107, 2387
Bsp19I CCATGG 2 cut(s) 440, 1507
BspACI CCGC 4 cut(s) 600, 990, 1119, 2006
BspANI GGCC 6 cut(s) 290, 346, 658, 783, 1506, 2079
BspCNI CTCAG 2 cut(s) 199, 1435
BspFNI CGCG 1 cut(s) 2006
BspHI TCATGA 1 cut(s) 2211
BspLI GGNNCC 5 cut(s) 71, 810, 847, 1649, 1943
BspMI ACCTGC 1 cut(s) 2275
BspPI GGATC 2 cut(s) 2060, 2102
BspT104I TTCGAA 1 cut(s) 2255
BspT107I GGYRCC 2 cut(s) 808, 845
BspTI CTTAAG 1 cut(s) 1099
BsrDI GCAATG 2 cut(s) 1879, 2315
BsrFI RCCGGY 3 cut(s) 162, 346, 2329
BsrGI TGTACA 2 cut(s) 1194, 2242
BsrI ACTGG 6 cut(s) 291, 514, 607, 644, 833, 1326
BssAI RCCGGY 3 cut(s) 162, 346, 2329
BssECI CCNNGG 7 cut(s) 51, 145, 440, 553, 902, 1507, 1936
BssMI GATC 8 cut(s) 589, 1262, 1450, 1858, 2052, 2098, 2107, 2387
BssSI CACGAG 3 cut(s) 543, 1488, 1806
BssT1I CCWWGG 2 cut(s) 440, 1507
Bst2BI CACGAG 3 cut(s) 543, 1488, 1806
Bst2UI CCWGG 5 cut(s) 52, 416, 480, 555, 903
Bst4CI ACNGT 3 cut(s) 211, 840, 1056
Bst6I CTCTTC 1 cut(s) 38
BstAFI CTTAAG 1 cut(s) 1099
BstAUI TGTACA 2 cut(s) 1194, 2242
BstBAI YACGTR 2 cut(s) 1222, 1226
BstBI TTCGAA 1 cut(s) 2255
BstC8I GCNNGC 5 cut(s) 367, 660, 1296, 1347, 2331
BstDEI CTNAG 4 cut(s) 186, 1422, 2164, 2322
BstDSI CCRYGG 3 cut(s) 440, 1507, 1936
BstF5I GGATG 3 cut(s) 94, 424, 962
BstFNI CGCG 1 cut(s) 2006
BstH2I RGCGCY 2 cut(s) 665, 1783
BstHHI GCGC 3 cut(s) 664, 1782, 2233
BstKTI GATC 8 cut(s) 592, 1265, 1453, 1861, 2055, 2101, 2110, 2390
BstMAI GTCTC 1 cut(s) 1420
BstMBI GATC 8 cut(s) 589, 1262, 1450, 1858, 2052, 2098, 2107, 2387
BstMCI CGRYCG 1 cut(s) 347
BstMWI GCNNNNNNNGC 6 cut(s) 25, 1253, 1322, 1628, 2276, 2326
BstNI CCWGG 5 cut(s) 52, 416, 480, 555, 903
BstNSI RCATGY 2 cut(s) 1714, 1993
BstSCI CCNGG 7 cut(s) 50, 145, 414, 478, 553, 818, 901
BstSFI CTRYAG 1 cut(s) 462
BstSNI TACGTA 1 cut(s) 1222
BstUI CGCG 1 cut(s) 2006
BstV1I GCAGC 3 cut(s) 1234, 1481, 2338
BstX2I RGATCY 3 cut(s) 1858, 2107, 2387
BstYI RGATCY 3 cut(s) 1858, 2107, 2387
BstZI CGGCCG 1 cut(s) 344
BsuRI GGCC 6 cut(s) 290, 346, 658, 783, 1506, 2079
BtgI CCRYGG 3 cut(s) 440, 1507, 1936
BtrI CACGTC 1 cut(s) 2132
BtsCI GGATG 3 cut(s) 94, 424, 962
BtsIMutI CAGTG 4 cut(s) 216, 845, 2183, 2358
BveI ACCTGC 1 cut(s) 2275
Cac8I GCNNGC 5 cut(s) 367, 660, 1296, 1347, 2331
CciI TCATGA 1 cut(s) 2211
CfoI GCGC 3 cut(s) 664, 1782, 2233
Cfr10I RCCGGY 3 cut(s) 162, 346, 2329
Cfr13I GGNCC 8 cut(s) 289, 327, 349, 656, 1330, 1758, 1942, 2260
Csp6I GTAC 5 cut(s) 637, 1195, 1223, 2002, 2243
CspAI ACCGGT 1 cut(s) 162
CviQI GTAC 5 cut(s) 637, 1195, 1223, 2002, 2243
DdeI CTNAG 4 cut(s) 186, 1422, 2164, 2322
DpnI GATC 8 cut(s) 591, 1264, 1452, 1860, 2054, 2100, 2109, 2389
DpnII GATC 8 cut(s) 589, 1262, 1450, 1858, 2052, 2098, 2107, 2387
DrdI GACNNNNNNGTC 1 cut(s) 2188
DseDI GACNNNNNNGTC 1 cut(s) 2188
EaeI YGGCCR 3 cut(s) 344, 1504, 2077
EagI CGGCCG 1 cut(s) 344
Eam1104I CTCTTC 1 cut(s) 38
EarI CTCTTC 1 cut(s) 38
Ecl136II GAGCTC 1 cut(s) 2143
EclXI CGGCCG 1 cut(s) 344
Eco105I TACGTA 1 cut(s) 1222
Eco130I CCWWGG 2 cut(s) 440, 1507
Eco24I GRGCYC 1 cut(s) 2145
Eco47I GGWCC 6 cut(s) 327, 349, 1330, 1758, 1942, 2260
Eco47III AGCGCT 1 cut(s) 1781
Eco52I CGGCCG 1 cut(s) 344
Eco53kI GAGCTC 1 cut(s) 2143
Eco57I CTGAAG 4 cut(s) 104, 124, 1569, 2207
EcoICRI GAGCTC 1 cut(s) 2143
EcoO109I RGGNCCY 1 cut(s) 1758
EcoRI GAATTC 1 cut(s) 1604
EcoRII CCWGG 5 cut(s) 50, 414, 478, 553, 901
EcoT14I CCWWGG 2 cut(s) 440, 1507
EcoT22I ATGCAT 1 cut(s) 2222
EcoT38I GRGCYC 1 cut(s) 2145
ErhI CCWWGG 2 cut(s) 440, 1507
FalI AAGNNNNNCTT 3 cut(s) 35, 1608, 1640
FaqI GGGAC 4 cut(s) 68, 578, 802, 1955
FauI CCCGC 1 cut(s) 983
FauNDI CATATG 2 cut(s) 1252, 1981
Fnu4HI GCNGC 4 cut(s) 600, 1248, 1470, 2327
FokI GGATG 3 cut(s) 101, 431, 969
FriOI GRGCYC 1 cut(s) 2145
Fsp4HI GCNGC 4 cut(s) 600, 1248, 1470, 2327
FspBI CTAG 7 cut(s) 156, 915, 1703, 1869, 1883, 1926, 2102
GlaI GCGC 3 cut(s) 663, 1781, 2232
GluI GCNGC 4 cut(s) 600, 1248, 1470, 2327
HaeII RGCGCY 2 cut(s) 665, 1783
HaeIII GGCC 6 cut(s) 290, 346, 658, 783, 1506, 2079
HapII CCGG 6 cut(s) 147, 163, 347, 820, 1997, 2330
HhaI GCGC 3 cut(s) 664, 1782, 2233
Hin6I GCGC 3 cut(s) 662, 1780, 2231
HinP1I GCGC 3 cut(s) 662, 1780, 2231
HincII GTYRAC 1 cut(s) 738
HindII GTYRAC 1 cut(s) 738
HindIII AAGCTT 1 cut(s) 1723
HinfI GANTC 9 cut(s) 47, 650, 743, 934, 1730, 1832, 2177, 2252, 2303
HpaII CCGG 6 cut(s) 147, 163, 347, 820, 1997, 2330
HphI GGTGA 4 cut(s) 508, 1817, 1986, 2312
Hpy166II GTNNAC 8 cut(s) 330, 738, 899, 1195, 1197, 1208, 2243, 2341
Hpy188I TCNGA 9 cut(s) 38, 189, 594, 939, 1425, 1912, 1976, 2035, 2356
Hpy8I GTNNAC 8 cut(s) 330, 738, 899, 1195, 1197, 1208, 2243, 2341
Hpy99I CGWCG 1 cut(s) 736
HpyAV CCTTC 9 cut(s) 79, 787, 794, 1136, 1749, 1771, 2121, 2147, 2251
HpyCH4III ACNGT 3 cut(s) 211, 840, 1056
HpyCH4IV ACGT 4 cut(s) 734, 1221, 1225, 2131
HpyF10VI GCNNNNNNNGC 6 cut(s) 25, 1253, 1322, 1628, 2276, 2326
HpyF3I CTNAG 4 cut(s) 186, 1422, 2164, 2322
HpySE526I ACGT 4 cut(s) 734, 1221, 1225, 2131
HspAI GCGC 3 cut(s) 662, 1780, 2231
KroI GCCGGC 1 cut(s) 2329
KroNI GCCGGC 1 cut(s) 2331
Kzo9I GATC 8 cut(s) 589, 1262, 1450, 1858, 2052, 2098, 2107, 2387
LmnI GCTCC 3 cut(s) 33, 1408, 2140
Lsp1109I GCAGC 3 cut(s) 1234, 1481, 2338
LweI GCATC 6 cut(s) 382, 1058, 1140, 1876, 2207, 2423
MaeI CTAG 7 cut(s) 156, 915, 1703, 1869, 1883, 1926, 2102
MaeII ACGT 4 cut(s) 734, 1221, 1225, 2131
MaeIII GTNAC 6 cut(s) 339, 540, 673, 824, 1992, 2407
MalI GATC 8 cut(s) 591, 1264, 1452, 1860, 2054, 2100, 2109, 2389
MboI GATC 8 cut(s) 589, 1262, 1450, 1858, 2052, 2098, 2107, 2387
MflI RGATCY 3 cut(s) 1858, 2107, 2387
MhlI GDGCHC 2 cut(s) 264, 2145
MlsI TGGCCA 2 cut(s) 1506, 2079
MluNI TGGCCA 2 cut(s) 1506, 2079
MlyI GAGTC 3 cut(s) 659, 1724, 1826
MmeI TCCRAC 2 cut(s) 1954, 2043
MnlI CCTC 9 cut(s) 41, 262, 675, 679, 1278, 1393, 1399, 1408, 1836
Mox20I TGGCCA 2 cut(s) 1506, 2079
Mph1103I ATGCAT 1 cut(s) 2222
MroNI GCCGGC 1 cut(s) 2329
MroXI GAANNNNTTC 3 cut(s) 1108, 2020, 2350
MscI TGGCCA 2 cut(s) 1506, 2079
MseI TTAA 8 cut(s) 62, 527, 755, 876, 1026, 1100, 2072, 2123
MslI CAYNNNNRTG 1 cut(s) 335
Msp20I TGGCCA 2 cut(s) 1506, 2079
MspCI CTTAAG 1 cut(s) 1099
MspI CCGG 6 cut(s) 147, 163, 347, 820, 1997, 2330
MspR9I CCNGG 7 cut(s) 52, 147, 416, 480, 555, 820, 903
MvaI CCWGG 5 cut(s) 52, 416, 480, 555, 903
MvnI CGCG 1 cut(s) 2006
MwoI GCNNNNNNNGC 6 cut(s) 25, 1253, 1322, 1628, 2276, 2326
NaeI GCCGGC 1 cut(s) 2331
NciI CCSGG 2 cut(s) 147, 820
NcoI CCATGG 2 cut(s) 440, 1507
NdeI CATATG 2 cut(s) 1252, 1981
NdeII GATC 8 cut(s) 589, 1262, 1450, 1858, 2052, 2098, 2107, 2387
NgoMIV GCCGGC 1 cut(s) 2329
NlaIV GGNNCC 5 cut(s) 71, 810, 847, 1649, 1943
NmuCI GTSAC 6 cut(s) 339, 540, 673, 824, 1992, 2407
NsiI ATGCAT 1 cut(s) 2222
NspI RCATGY 2 cut(s) 1714, 1993
NspV TTCGAA 1 cut(s) 2255
OliI CACNNNNGTG 1 cut(s) 335
PagI TCATGA 1 cut(s) 2211
PciI ACATGT 1 cut(s) 1989
PdiI GCCGGC 1 cut(s) 2331
PdmI GAANNNNTTC 3 cut(s) 1108, 2020, 2350
PfeI GAWTC 6 cut(s) 47, 743, 934, 2177, 2252, 2303
Pfl23II CGTACG 1 cut(s) 1222
PfoI TCCNGGA 1 cut(s) 414
PinAI ACCGGT 1 cut(s) 162
PkrI GCNGC 4 cut(s) 601, 1249, 1471, 2328
PleI GAGTC 3 cut(s) 658, 1724, 1826
PpsI GAGTC 3 cut(s) 658, 1724, 1826
Ppu21I YACGTR 2 cut(s) 1222, 1226
PpuMI RGGWCCY 1 cut(s) 1758
PscI ACATGT 1 cut(s) 1989
PshBI ATTAAT 1 cut(s) 876
PsiI TTATAA 2 cut(s) 303, 1530
Psp124BI GAGCTC 1 cut(s) 2145
Psp5II RGGWCCY 1 cut(s) 1758
Psp6I CCWGG 5 cut(s) 50, 414, 478, 553, 901
PspGI CCWGG 5 cut(s) 50, 414, 478, 553, 901
PspLI CGTACG 1 cut(s) 1222
PspN4I GGNNCC 5 cut(s) 71, 810, 847, 1649, 1943
PspPI GGNCC 8 cut(s) 289, 327, 349, 656, 1330, 1758, 1942, 2260
PspPPI RGGWCCY 1 cut(s) 1758
PsuI RGATCY 3 cut(s) 1858, 2107, 2387
RsaI GTAC 5 cut(s) 638, 1196, 1224, 2003, 2244
RsaNI GTAC 5 cut(s) 637, 1195, 1223, 2002, 2243
RseI CAYNNNNRTG 1 cut(s) 335
SacI GAGCTC 1 cut(s) 2145
SaqAI TTAA 8 cut(s) 62, 527, 755, 876, 1026, 1100, 2072, 2123
SatI GCNGC 4 cut(s) 600, 1248, 1470, 2327
Sau3AI GATC 8 cut(s) 589, 1262, 1450, 1858, 2052, 2098, 2107, 2387
Sau96I GGNCC 8 cut(s) 289, 327, 349, 656, 1330, 1758, 1942, 2260
ScaI AGTACT 1 cut(s) 638
SchI GAGTC 3 cut(s) 659, 1724, 1826
ScrFI CCNGG 7 cut(s) 52, 147, 416, 480, 555, 820, 903
SduI GDGCHC 2 cut(s) 264, 2145
SfaNI GCATC 6 cut(s) 382, 1058, 1140, 1876, 2207, 2423
SfcI CTRYAG 1 cut(s) 462
SfuI TTCGAA 1 cut(s) 2255
SinI GGWCC 6 cut(s) 327, 349, 1330, 1758, 1942, 2260
SmiMI CAYNNNNRTG 1 cut(s) 335
SmlI CTYRAG 3 cut(s) 1099, 1412, 1834
SmoI CTYRAG 3 cut(s) 1099, 1412, 1834
SnaBI TACGTA 1 cut(s) 1222
SsiI CCGC 4 cut(s) 600, 990, 1119, 2006
SspI AATATT 3 cut(s) 874, 1308, 1932
SspMI CTAG 7 cut(s) 156, 915, 1703, 1869, 1883, 1926, 2102
SstI GAGCTC 1 cut(s) 2145
StyD4I CCNGG 7 cut(s) 50, 145, 414, 478, 553, 818, 901
StyI CCWWGG 2 cut(s) 440, 1507
TaaI ACNGT 3 cut(s) 211, 840, 1056
TaiI ACGT 4 cut(s) 737, 1224, 1228, 2134
TaqI TCGA 6 cut(s) 126, 1134, 1449, 2180, 2255, 2431
TaqII GACCGA 2 cut(s) 755, 2277
TatI WGTACW 3 cut(s) 636, 1194, 2242
TauI GCSGC 1 cut(s) 602
TfiI GAWTC 6 cut(s) 47, 743, 934, 2177, 2252, 2303
Tru1I TTAA 8 cut(s) 62, 527, 755, 876, 1026, 1100, 2072, 2123
Tru9I TTAA 8 cut(s) 62, 527, 755, 876, 1026, 1100, 2072, 2123
TscAI CASTG 4 cut(s) 216, 845, 2190, 2365
TseFI GTSAC 6 cut(s) 339, 540, 673, 824, 1992, 2407
TseI GCWGC 3 cut(s) 1247, 1469, 2326
Tsp45I GTSAC 6 cut(s) 339, 540, 673, 824, 1992, 2407
TspGWI ACGGA 1 cut(s) 1812
TspRI CASTG 4 cut(s) 216, 845, 2190, 2365
Vha464I CTTAAG 1 cut(s) 1099
VpaK11BI GGWCC 6 cut(s) 327, 349, 1330, 1758, 1942, 2260
VspI ATTAAT 1 cut(s) 876
XapI RAATTY 7 cut(s) 151, 174, 400, 1028, 1604, 1905, 2350
XbaI TCTAGA 1 cut(s) 2101
XceI RCATGY 2 cut(s) 1714, 1993
XcmI CCANNNNNNNNNTGG 2 cut(s) 1514, 2087
XmnI GAANNNNTTC 3 cut(s) 1108, 2020, 2350
XspI CTAG 7 cut(s) 156, 915, 1703, 1869, 1883, 1926, 2102
ZrmI AGTACT 1 cut(s) 638
Zsp2I ATGCAT 1 cut(s) 2222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.