Rroxscaffold_6G00405020

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000112
Physical Location & Seq
Reverse (-)
27501982 .. 27506369
4388 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00405020.1

Sequence Viewer

Length: 2580 bp
ATGGCCCGTCGGGGACAATATTCTATTCCATTTATTGCTCCTCCTCCAGTGGTGGAACTTCTTGATGATCCTTTTGAATCAAATATCGCTGATCAGGGAAGAAGATGCCTACTAGGACAAGTTTTTGGAGAGCCGATCTCAGTTAAGAGTGTTAGAGCAAGTCCACCCGTTAAGATTTCGTGCTTAGCCGGTTGGAAAGGCCTTCTTGGAAGCGTTCGGATTAATAATAGCTTTGGACCTAACTGGTTCAGGGAAATGTTTGATTATGAGGAGGATTTCATTCATGTTCTTAACAACAAGCCATGTTGCAGGGACACCATCAGATGGAATGAACAACAATTGAAGGATGTTGATGGTGAAGTTTTGGTGTCCAAAGGAAGCAACTTTGAGTTGGGTTTCTTCAGCCCCGGCGATTCTAGCCACCGGTATGGAGAATTAGCTCTTTATGCTTATAGCATGGAGAACATTCCTATATGGTCCACAAATGCGTCACAGTCGGTCCAAAACACAGACACCTTAAATAGATATGTACAGCTTTTAGATACCGGAAACTTAGTCGTGTTCCGGGATGGTAGAAATGGAAGCTTGATTTTATGGCAAAGTTTTGATTATCCTACAGATACTCTAATCCCAGGTATGAAATTGGGGTTGAATTGGAAAAAAGGGCTAGATTGGCATTTAACATCTTGGAAGTCACAAGATGACCCTGGAAAGGGGGACTATGCCTTTAGGGTCGATCCGAATCACAATGCTACTCCCCAATATTTTGTCTACAAGGGTTCCACTAAGTATTGGCGAGTGGATGCAGGGCCATGGCCTAATTTTGTTAGTAATGCCAACGAAATGTATATTACCCTGCCTTATAAGAGCAATGCTATTTCGATACTAAAGTTGAAGGATTCCGGGTTATTTCAGCACCTACTATGGAATGATGCTGACCATCAATGGGTGGAAATGTGGTCTTCGCCGAAATACCCTTGTGACTGGTATGGACATTGTGGTGCCAACAGCAAATGTAGCCCTGACAATATTAATTTATTTGAGTGTGAGTGTATTCCAGGGTATGAGCCAAAATCTGTAGATGGTTGGAATCGGAGAGATGGAACAGATGGATGTGTGAGTAAACGAGTAAGAGTGTCAAAGTGTAGGAATGGAGAAGGGTTTGTGAAGGTGGCAAGGGTAAAAGATCCAGACACATCGAATGTAGCACGGCTGAGAACAAGTATGAGTGCCAAAGAGTGTGAGCAGGAGTGCTTGAAAAATTGTTCCTGCACGGCGTATATGAGCATTACAATGGAAAGGGTTGTCAATTGCTTGACATGGTATGATGACTTGATGGACATGTTAGTTTACACAGAGGTCGGACGAGATCTATATGTTCGTGTGGATAAAATCTCATTAGCTGAGGCCAGAAACTCACAAGGTTTCTTCAGGAGTGGCAAGGTCCTTATTCCAATACTAACTGCTTTGGTGACACTACTACTAATCATTATACTTTCCTTGTGGTTGCTTAAGAAAAAGAGGAAGACAAGAGACTATTTGGATGCGGAGGAACTTGAGGAAAGTAAGAGACACCCGGAATTGCAATTTTTCGATCTTACCACACTAATTGCAGCCACAGACAACTTCTCTGATGCCAATAAACTTGGCCAAGGTGGTTTTGGCATTGTTTATAAGGGTGAGTTACCAAACGAACAAAAAGTTGCTATAAAAAGATTGTCCAGAACTTCAGGACAGGGAATCAAAGAATTCAAAAATGAAGTTGCACTAATAGCCAGACTTCAACACAGGAATCTTGTGAAACTTTTAGGCTGCTGTATAAAGGGGGAAGAAAGGATATTGGTTCTGGAATACATGCCGAACAAAAGCTTGGACTCCTTTCTTTTTGATAACACAAGGAGGTCACTCTTAAATTGGGAAAAGCGTTTTGAAATTATCAATGGGGTTGCTCGTGGGGTTCTATATCTTCATCGGGACTCAAGATTGAGGATTATTCACAGGGATCTGAAAACTAGTAATGTCCTTTTAGATGCCGAGTTAGACCCAAAAATTTCTGATTTCGGCATGGCAAGAATATTTCAAGGGGACCAACTCCTGGAAAAGACAAACAGAGTTGTTGGAACATACGGCTACATGTCACCAGAATATGCAGTATTTGGGAGATTTTCTACGAAATCGGATGTGTTTAGTTTTGGGATCATACTATTGGAGATTGTTAGTGGCAAGAGAAACAATGGTTCTTATGAGATGCATTATTCCGAAAACTTGATAGGACATGTATGGCAACTTTGGGTAGAAGACAAAGTCTTGGAAATTGTGGATTCATCACTAGAGATTTATCAGCTTGATGAAGTCAGGAGATGCATTCAAGTTGGGCTTTTGTGCGTGCAAGAAGATTTGAAGGACCGACCAGCCATGTCAGCCGTCGTGTTCATGTTGAGTGGTGAAACATCTCTTCCATCTCCTAAGCAGCCTGCATTTGTTTTCAGAACAAGTTCCAGCATTGTTGATGATCCCTTCATTCCAAATGAATCATATTCTAGTAATAGTTTGACAATAACTAACATGGAAGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

859

Amino Acids

97.87

Weight (kDa)

6.42

Isoelectric Point (pI)

42.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 144 - 231 1.5e-21 D-mannose binding lectin
S_locus_glycop PF00954 274 - 359 3.1e-15 S-locus glycoprotein domain
PAN_2 PF08276 382 - 448 5.3e-16 PAN-like domain
Pkinase PF00069 544 - 746 7.5e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 547 - 810 4e-49 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 816 - 859 3.9e-06 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 864, 1674
AccB1I GGYRCC 1 cut(s) 1001
AccB7I CCANNNNNTGG 2 cut(s) 324, 2095
AccI GTMKAC 1 cut(s) 771
AciI CCGC 1 cut(s) 1547
AclWI GGATC 6 cut(s) 62, 731, 1181, 2010, 2204, 2507
AcoI YGGCCR 1 cut(s) 1648
AcsI RAATTY 2 cut(s) 1748, 2049
AcuI CTGAAG 3 cut(s) 385, 1414, 1713
AfaI GTAC 1 cut(s) 531
AfiI CCNNNNNNNGG 5 cut(s) 324, 712, 713, 946, 2095
AflII CTTAAG 1 cut(s) 1511
AflIII ACRYGT 3 cut(s) 1341, 2133, 2275
AgeI ACCGGT 1 cut(s) 423
AhlI ACTAGT 1 cut(s) 2012
AjnI CCWGG 4 cut(s) 631, 706, 1057, 2094
AjuI GAANNNNNNNTTGG 2 cut(s) 1853, 1885
AluBI AGCT 8 cut(s) 231, 440, 535, 585, 1403, 1869, 2344, 2573
AluI AGCT 8 cut(s) 231, 440, 535, 585, 1403, 1869, 2344, 2573
Alw26I GTCTC 2 cut(s) 1527, 1564
AlwI GGATC 6 cut(s) 62, 731, 1181, 2010, 2204, 2507
AoxI GGCC 6 cut(s) 3, 199, 809, 815, 1407, 1648
ApeKI GCWGC 3 cut(s) 1613, 1812, 2470
ApoI RAATTY 2 cut(s) 1748, 2049
AseI ATTAAT 2 cut(s) 222, 1032
AsiGI ACCGGT 1 cut(s) 423
Asp700I GAANNNNTTC 1 cut(s) 2494
AspS9I GGNCC 8 cut(s) 4, 236, 477, 499, 809, 1444, 2086, 2404
AsuC2I CCSGG 4 cut(s) 408, 566, 904, 1577
AsuHPI GGTGA 5 cut(s) 368, 1483, 1691, 2130, 2456
AvaII GGWCC 6 cut(s) 236, 477, 499, 1444, 2086, 2404
BaeI ACNNNNGTAYC 2 cut(s) 875, 908
BalI TGGCCA 1 cut(s) 1650
BanI GGYRCC 1 cut(s) 1001
BauI CACGAG 1 cut(s) 1950
BbsI GAAGAC 3 cut(s) 954, 1532, 2304
BbvCI CCTCAGC 1 cut(s) 1404
BbvI GCAGC 3 cut(s) 1625, 1799, 2482
BceAI ACGGC 4 cut(s) 1226, 1290, 2143, 2408
BciT130I CCWGG 4 cut(s) 633, 708, 1059, 2096
BclI TGATCA 1 cut(s) 91
BcnI CCSGG 4 cut(s) 408, 566, 904, 1577
BcoDI GTCTC 2 cut(s) 1527, 1564
BcuI ACTAGT 1 cut(s) 2012
BfaI CTAG 6 cut(s) 113, 417, 668, 2013, 2330, 2541
BfmI CTRYAG 2 cut(s) 615, 1077
BfrI CTTAAG 1 cut(s) 1511
BglII AGATCT 1 cut(s) 1369
BisI GCNGC 3 cut(s) 1614, 1813, 2471
BlpI GCTNAGC 1 cut(s) 184
BlsI GCNGC 3 cut(s) 1615, 1814, 2472
Bme1390I CCNGG 8 cut(s) 408, 566, 633, 708, 904, 1059, 1577, 2096
Bme18I GGWCC 6 cut(s) 236, 477, 499, 1444, 2086, 2404
BmgT120I GGNCC 8 cut(s) 4, 236, 477, 499, 809, 1444, 2086, 2404
BmiI GGNNCC 3 cut(s) 781, 1003, 2087
BmrFI CCNGG 8 cut(s) 408, 566, 633, 708, 904, 1059, 1577, 2096
BmsI GCATC 8 cut(s) 95, 793, 922, 1534, 1624, 2020, 2238, 2351
BpiI GAAGAC 3 cut(s) 954, 1532, 2304
BplI GAGNNNNNCTC 2 cut(s) 122, 154
BpmI CTGGAG 1 cut(s) 30
Bpu10I CCTNAGC 2 cut(s) 1404, 2466
Bpu1102I GCTNAGC 1 cut(s) 184
BpuEI CTTGAG 2 cut(s) 1577, 1963
BpuMI CCSGG 4 cut(s) 408, 566, 904, 1577
BsaBI GATNNNNATC 1 cut(s) 741
BsaJI CCNNGG 6 cut(s) 406, 631, 706, 812, 1058, 1651
BsaWI WCCGGW 2 cut(s) 423, 545
Bsc4I CCNNNNNNNGG 5 cut(s) 324, 712, 713, 946, 2095
Bse118I RCCGGY 2 cut(s) 188, 423
Bse1I ACTGG 3 cut(s) 47, 248, 989
Bse3DI GCAATG 1 cut(s) 877
Bse8I GATNNNNATC 1 cut(s) 741
BseBI CCWGG 4 cut(s) 633, 708, 1059, 2096
BseDI CCNNGG 6 cut(s) 406, 631, 706, 812, 1058, 1651
BseGI GGATG 6 cut(s) 352, 574, 808, 1118, 1549, 2185
BseJI GATNNNNATC 1 cut(s) 741
BseLI CCNNNNNNNGG 5 cut(s) 324, 712, 713, 946, 2095
BseMI GCAATG 1 cut(s) 877
BseMII CTCAG 3 cut(s) 153, 1205, 1395
BseNI ACTGG 3 cut(s) 47, 248, 989
BseRI GAGGAG 3 cut(s) 30, 33, 284
BseXI GCAGC 3 cut(s) 1625, 1799, 2482
BsgI GTGCAG 1 cut(s) 1255
BshFI GGCC 6 cut(s) 5, 201, 811, 817, 1409, 1650
BshNI GGYRCC 1 cut(s) 1001
BshTI ACCGGT 1 cut(s) 423
BsiSI CCGG 7 cut(s) 189, 408, 424, 546, 565, 903, 1577
BslFI GGGAC 5 cut(s) 27, 326, 731, 1988, 2099
BslI CCNNNNNNNGG 5 cut(s) 324, 712, 713, 946, 2095
BsmAI GTCTC 2 cut(s) 1527, 1564
BsmFI GGGAC 5 cut(s) 27, 326, 731, 1988, 2099
BsmI GAATGC 1 cut(s) 2364
BsnI GGCC 6 cut(s) 5, 201, 811, 817, 1409, 1650
Bsp1407I TGTACA 1 cut(s) 529
Bsp1720I GCTNAGC 1 cut(s) 184
Bsp19I CCATGG 1 cut(s) 812
BspACI CCGC 1 cut(s) 1547
BspANI GGCC 6 cut(s) 5, 201, 811, 817, 1409, 1650
BspCNI CTCAG 3 cut(s) 152, 1206, 1396
BspLI GGNNCC 3 cut(s) 781, 1003, 2087
BspPI GGATC 6 cut(s) 62, 731, 1181, 2010, 2204, 2507
BspT107I GGYRCC 1 cut(s) 1001
BspTI CTTAAG 1 cut(s) 1511
BsrDI GCAATG 1 cut(s) 877
BsrFI RCCGGY 2 cut(s) 188, 423
BsrGI TGTACA 1 cut(s) 529
BsrI ACTGG 3 cut(s) 47, 248, 989
BssAI RCCGGY 2 cut(s) 188, 423
BssECI CCNNGG 6 cut(s) 406, 631, 706, 812, 1058, 1651
BssSI CACGAG 1 cut(s) 1950
BssT1I CCWWGG 2 cut(s) 812, 1651
Bst2BI CACGAG 1 cut(s) 1950
Bst2UI CCWGG 4 cut(s) 633, 708, 1059, 2096
Bst4CI ACNGT 1 cut(s) 495
Bst6I CTCTTC 1 cut(s) 2460
BstAFI CTTAAG 1 cut(s) 1511
BstAUI TGTACA 1 cut(s) 529
BstC8I GCNNGC 2 cut(s) 2387, 2475
BstDEI CTNAG 7 cut(s) 139, 184, 553, 786, 1214, 1404, 2466
BstDSI CCRYGG 1 cut(s) 812
BstF5I GGATG 6 cut(s) 352, 574, 808, 1118, 1549, 2185
BstMAI GTCTC 2 cut(s) 1527, 1564
BstMWI GCNNNNNNNGC 6 cut(s) 417, 446, 673, 1017, 1772, 2420
BstNI CCWGG 4 cut(s) 633, 708, 1059, 2096
BstNSI RCATGY 4 cut(s) 1345, 1858, 2137, 2279
BstSCI CCNGG 8 cut(s) 406, 564, 631, 706, 902, 1057, 1575, 2094
BstSFI CTRYAG 2 cut(s) 615, 1077
BstV1I GCAGC 3 cut(s) 1625, 1799, 2482
BstV2I GAAGAC 3 cut(s) 954, 1532, 2304
BstX2I RGATCY 3 cut(s) 1186, 1369, 2002
BstXI CCANNNNNNTGG 1 cut(s) 428
BstYI RGATCY 3 cut(s) 1186, 1369, 2002
BsuRI GGCC 6 cut(s) 5, 201, 811, 817, 1409, 1650
BtgI CCRYGG 1 cut(s) 812
BtsCI GGATG 6 cut(s) 352, 574, 808, 1118, 1549, 2185
BtsIMutI CAGTG 1 cut(s) 54
Cac8I GCNNGC 2 cut(s) 2387, 2475
Cfr10I RCCGGY 2 cut(s) 188, 423
Cfr13I GGNCC 8 cut(s) 4, 236, 477, 499, 809, 1444, 2086, 2404
CseI GACGC 1 cut(s) 477
Csp6I GTAC 1 cut(s) 530
CspAI ACCGGT 1 cut(s) 423
CspCI CAANNNNNGTGG 2 cut(s) 1591, 1626
CviQI GTAC 1 cut(s) 530
DdeI CTNAG 7 cut(s) 139, 184, 553, 786, 1214, 1404, 2466
EaeI YGGCCR 1 cut(s) 1648
Eam1104I CTCTTC 1 cut(s) 2460
EarI CTCTTC 1 cut(s) 2460
Eco130I CCWWGG 2 cut(s) 812, 1651
Eco147I AGGCCT 1 cut(s) 201
Eco47I GGWCC 6 cut(s) 236, 477, 499, 1444, 2086, 2404
Eco57I CTGAAG 3 cut(s) 385, 1414, 1713
EcoO109I RGGNCCY 1 cut(s) 1444
EcoRI GAATTC 1 cut(s) 1748
EcoRII CCWGG 4 cut(s) 631, 706, 1057, 2094
EcoT14I CCWWGG 2 cut(s) 812, 1651
EcoT22I ATGCAT 2 cut(s) 2253, 2366
ErhI CCWWGG 2 cut(s) 812, 1651
FalI AAGNNNNNCTT 4 cut(s) 189, 221, 2361, 2393
FaqI GGGAC 5 cut(s) 27, 326, 731, 1988, 2099
FbaI TGATCA 1 cut(s) 91
FblI GTMKAC 1 cut(s) 771
Fnu4HI GCNGC 3 cut(s) 1614, 1813, 2471
FokI GGATG 6 cut(s) 359, 581, 815, 1125, 1556, 2192
Fsp4HI GCNGC 3 cut(s) 1614, 1813, 2471
FspBI CTAG 6 cut(s) 113, 417, 668, 2013, 2330, 2541
GluI GCNGC 3 cut(s) 1614, 1813, 2471
GsuI CTGGAG 1 cut(s) 30
HaeIII GGCC 6 cut(s) 5, 201, 811, 817, 1409, 1650
HapII CCGG 7 cut(s) 189, 408, 424, 546, 565, 903, 1577
HgaI GACGC 1 cut(s) 477
HindIII AAGCTT 2 cut(s) 583, 1867
HpaII CCGG 7 cut(s) 189, 408, 424, 546, 565, 903, 1577
HphI GGTGA 5 cut(s) 368, 1483, 1691, 2130, 2456
Hpy166II GTNNAC 5 cut(s) 164, 480, 772, 1124, 1351
Hpy188III TCNNGA 9 cut(s) 62, 1190, 1432, 1722, 1731, 1847, 1973, 1980, 2356
Hpy8I GTNNAC 5 cut(s) 164, 480, 772, 1124, 1351
Hpy99I CGWCG 2 cut(s) 12, 2429
HpyAV CCTTC 7 cut(s) 212, 337, 889, 1151, 1162, 2395, 2527
HpyCH4III ACNGT 1 cut(s) 495
HpyF10VI GCNNNNNNNGC 6 cut(s) 417, 446, 673, 1017, 1772, 2420
HpyF3I CTNAG 7 cut(s) 139, 184, 553, 786, 1214, 1404, 2466
Ksp22I TGATCA 1 cut(s) 91
LmnI GCTCC 1 cut(s) 43
Lsp1109I GCAGC 3 cut(s) 1625, 1799, 2482
LweI GCATC 8 cut(s) 95, 793, 922, 1534, 1624, 2020, 2238, 2351
MaeI CTAG 6 cut(s) 113, 417, 668, 2013, 2330, 2541
MaeIII GTNAC 7 cut(s) 489, 693, 980, 1471, 1683, 1902, 2136
MfeI CAATTG 2 cut(s) 338, 1309
MflI RGATCY 3 cut(s) 1186, 1369, 2002
MlsI TGGCCA 1 cut(s) 1650
MluNI TGGCCA 1 cut(s) 1650
MlyI GAGTC 2 cut(s) 1868, 1970
MmeI TCCRAC 4 cut(s) 173, 1067, 1342, 2098
Mox20I TGGCCA 1 cut(s) 1650
Mph1103I ATGCAT 2 cut(s) 2253, 2366
MroXI GAANNNNTTC 1 cut(s) 2494
MscI TGGCCA 1 cut(s) 1650
MseI TTAA 9 cut(s) 144, 171, 222, 291, 518, 680, 1032, 1512, 1910
MslI CAYNNNNRTG 3 cut(s) 426, 999, 1292
Msp20I TGGCCA 1 cut(s) 1650
MspCI CTTAAG 1 cut(s) 1511
MspI CCGG 7 cut(s) 189, 408, 424, 546, 565, 903, 1577
MspR9I CCNGG 8 cut(s) 408, 566, 633, 708, 904, 1059, 1577, 2096
MunI CAATTG 2 cut(s) 338, 1309
Mva1269I GAATGC 1 cut(s) 2364
MvaI CCWGG 4 cut(s) 633, 708, 1059, 2096
MwoI GCNNNNNNNGC 6 cut(s) 417, 446, 673, 1017, 1772, 2420
NciI CCSGG 4 cut(s) 408, 566, 904, 1577
NcoI CCATGG 1 cut(s) 812
NlaIV GGNNCC 3 cut(s) 781, 1003, 2087
NmeAIII GCCGAG 1 cut(s) 2059
NmuCI GTSAC 6 cut(s) 489, 693, 980, 1471, 1902, 2136
NsiI ATGCAT 2 cut(s) 2253, 2366
NspI RCATGY 4 cut(s) 1345, 1858, 2137, 2279
PceI AGGCCT 1 cut(s) 201
PciI ACATGT 3 cut(s) 1341, 2133, 2275
PctI GAATGC 1 cut(s) 2364
PdmI GAANNNNTTC 1 cut(s) 2494
PfeI GAWTC 9 cut(s) 77, 413, 742, 899, 1090, 1740, 1792, 2321, 2531
PflFI GACNNNGTC 1 cut(s) 2303
PflMI CCANNNNNTGG 2 cut(s) 324, 2095
PfoI TCCNGGA 2 cut(s) 564, 2094
PinAI ACCGGT 1 cut(s) 423
PkrI GCNGC 3 cut(s) 1615, 1814, 2472
PleI GAGTC 2 cut(s) 1868, 1970
PpsI GAGTC 2 cut(s) 1868, 1970
PpuMI RGGWCCY 1 cut(s) 1444
PscI ACATGT 3 cut(s) 1341, 2133, 2275
PshBI ATTAAT 2 cut(s) 222, 1032
PsiI TTATAA 2 cut(s) 864, 1674
Psp5II RGGWCCY 1 cut(s) 1444
Psp6I CCWGG 4 cut(s) 631, 706, 1057, 2094
PspGI CCWGG 4 cut(s) 631, 706, 1057, 2094
PspN4I GGNNCC 3 cut(s) 781, 1003, 2087
PspPI GGNCC 8 cut(s) 4, 236, 477, 499, 809, 1444, 2086, 2404
PspPPI RGGWCCY 1 cut(s) 1444
PsuI RGATCY 3 cut(s) 1186, 1369, 2002
PsyI GACNNNGTC 1 cut(s) 2303
RsaI GTAC 1 cut(s) 531
RsaNI GTAC 1 cut(s) 530
RseI CAYNNNNRTG 3 cut(s) 426, 999, 1292
SaqAI TTAA 9 cut(s) 144, 171, 222, 291, 518, 680, 1032, 1512, 1910
SatI GCNGC 3 cut(s) 1614, 1813, 2471
Sau96I GGNCC 8 cut(s) 4, 236, 477, 499, 809, 1444, 2086, 2404
SchI GAGTC 2 cut(s) 1868, 1970
ScrFI CCNGG 8 cut(s) 408, 566, 633, 708, 904, 1059, 1577, 2096
SfaNI GCATC 8 cut(s) 95, 793, 922, 1534, 1624, 2020, 2238, 2351
SfcI CTRYAG 2 cut(s) 615, 1077
SinI GGWCC 6 cut(s) 236, 477, 499, 1444, 2086, 2404
SmiMI CAYNNNNRTG 3 cut(s) 426, 999, 1292
SmlI CTYRAG 3 cut(s) 1511, 1556, 1978
SmoI CTYRAG 3 cut(s) 1511, 1556, 1978
SpeI ACTAGT 1 cut(s) 2012
SseBI AGGCCT 1 cut(s) 201
SsiI CCGC 1 cut(s) 1547
SspI AATATT 4 cut(s) 20, 764, 1030, 2076
SspMI CTAG 6 cut(s) 113, 417, 668, 2013, 2330, 2541
StuI AGGCCT 1 cut(s) 201
StyD4I CCNGG 8 cut(s) 406, 564, 631, 706, 902, 1057, 1575, 2094
StyI CCWWGG 2 cut(s) 812, 1651
TaaI ACNGT 1 cut(s) 495
TaqI TCGA 5 cut(s) 735, 881, 1199, 1593, 2575
TaqII GACCGA 2 cut(s) 487, 2421
TatI WGTACW 1 cut(s) 529
TfiI GAWTC 9 cut(s) 77, 413, 742, 899, 1090, 1740, 1792, 2321, 2531
Tru1I TTAA 9 cut(s) 144, 171, 222, 291, 518, 680, 1032, 1512, 1910
Tru9I TTAA 9 cut(s) 144, 171, 222, 291, 518, 680, 1032, 1512, 1910
TscAI CASTG 1 cut(s) 54
TseFI GTSAC 6 cut(s) 489, 693, 980, 1471, 1902, 2136
TseI GCWGC 3 cut(s) 1613, 1812, 2470
Tsp45I GTSAC 6 cut(s) 489, 693, 980, 1471, 1902, 2136
TspRI CASTG 1 cut(s) 54
Tth111I GACNNNGTC 1 cut(s) 2303
Van91I CCANNNNNTGG 2 cut(s) 324, 2095
Vha464I CTTAAG 1 cut(s) 1511
VpaK11BI GGWCC 6 cut(s) 236, 477, 499, 1444, 2086, 2404
VspI ATTAAT 2 cut(s) 222, 1032
XapI RAATTY 2 cut(s) 1748, 2049
XceI RCATGY 4 cut(s) 1345, 1858, 2137, 2279
XcmI CCANNNNNNNNNTGG 1 cut(s) 1658
XmiI GTMKAC 1 cut(s) 771
XmnI GAANNNNTTC 1 cut(s) 2494
XspI CTAG 6 cut(s) 113, 417, 668, 2013, 2330, 2541
Zsp2I ATGCAT 2 cut(s) 2253, 2366
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.