RLG00000031292

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000112
Physical Location & Seq
Reverse (-)
3799938 .. 3803464
3527 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031292

Sequence Viewer

Length: 2217 bp
ATGTTACTGAAATCTCTGCTTCTCATCCTCCTTTTCCAACTGTCCACTTCCAAGGACACCATAACATGGAACGGAGAGCAAGTAAAGGATGGTGACCTTCTAGTGTCCAAAGGAAACAGCTTTGAATTGGGTTTCTTCAGCCCCAGAAACTCGAGCTACCGCTATGTTGGAATTTGGTATTCCATGTCTCAGATATCTGACCAAACAGTGGTGTGGGTGGCAAACAGAAACAACCCCATCAATGATACATCAGGTGTTCTCACAATAAACAGGTATGGAAAACTAGTCATTTACGCTCACAATATGGAGAACGTTCCTATATGGTCTGCCAATATGTCTCGGTCAGTCATAAACACAAACCTCTTATCTGCACAACTTTTAGATACAGGAAACTTAGTTCTGACCCAGGATGGTAAAACTGAAAGCTTTATTATATGGCAGAGTTTTGATTATCCTACAGATACTCTAATCCCTGGTATGAAACTTGGGGTGAGTTGGAAAACTGGTTTAGAGTGGTTTTTAACATCTTGGAAGTCACAAGATGACCCTGGAACTGGGGACTATAGCCATAGGCTTCATTGGAACCAGACGGCTATGCTACAATATTTTCTGTTCAAGGGTTTAACTACTAGGGTTTGGAGAAGTGATCCTGGGCCTTGGCGCAGTTTTGTTAGTAATAATGATGAAACGTATTACCTACAATCTAGTGATAACTCCACTACAAAATTTAGAATGGTTGTGAATGATTCTGGGTTATTGCAGCATTTGACATGGAATGATGGTGACCATCAATGGAAGGAACTATGGGCTGCACCGAAATACCGTTGTGACTTGTATGGACAGTGTGGTGCCTATAGTACATGTAACCCTGACAATATTAATATGTTTGAGTGTGAGTGTTTACCAGGGTATGAACCTAAATCTATAAATGACTGGAATCAGAATGATGGGTCAGAGGGGTGTCTGAGTAAGCGAGTTGGAGTGTCAAAGTGTGGAGTTGGAGATGGGTTTTTAGAGGTGGCAAGGGTGAAAGCTCCAGACACATCGACAGTAGTAGCACGACTGGATACTGGTATGAGTGCCAAAGAGTGCAAGCAGGAATGTTTGAGAAATTGTTCTTGCACGGCATGTATGAGCATCAATAATAAAGGGCGCATTGATTGCTTGACATGGTATGGTGAGCTGCTGGATATTGTGAAGCACACGGATGGAGGACAAGTTCTACATGTTCGTGTGGATGAAATTGAGTTAGCAAACATTATTGTGCATGAGGAACTTCATCTTTACAACTTCTGTGAGTGTTTAGAAGAGTTAAAAGGTCAGTTACCAAATGGACAGGAGATTGCTGTGAAAAGATTGTCCAAAACTTCAGGGCAAGGGATTGAAGAATTCAAAAATGAAGTTGTGCTTATAGAAAGACTTCAACACAGGAACCTTGTTAAACTTTTGGGTTGTTGCATAGCGGGAGAAGAAAAGATGTTGGTCCTTGAATACTTGTCGAACAAAAGCTTGGACTTCTTTCTTTTTGATGACACAAGAAGGTCCTCTTTGGGTTGGAAAAAGCGCTTTGAAATCATCAATGGGATTGCTCGTGGGATTCTTTATCTTCACCAGGACTCGAGAGTTAGAATTATACATAGGGATCTAAAAACCAGCAATATTCTTCTAGATGCTGAGATGAACCCAAAAATTTCAGATTTTGGCATGGCAAGAATATTTTATGGGGACCAATTGCAGGATAAGACAGCCAGAGTTGTTGGAACATATGGGTATATGTCACCAGAGTATGCAGTTTTTGGTAGATATTCAACAAAATCAGATGTCTTTAGCTTCGGGATCATAATATTGGAGATCATAAGCGGCAAGAAAAACTCTGGTTCTTATTGGGAGGATCATTCCATGAACTTGATAGGAAATGTTTGGCAGATGTGGGGTGAAGACAGAGCTTTAGAAATTATGGATTCATCACTAGAGTTATACCAACCAGATGAAGTCTTGAGATGCATTCAAGTTGGGCTCTTATGTGTGCAAGAAGATTCAAAGAACCGGCCTACCATGTCAGATGCTGTTTTCATGTTGAGTGGTGAAGCATCTCTTTCAACTCCTAACCAGCCTGCATTTGTTTTTAGAAAAAGTGCATGCAGTAATGTTCCATCCCTTGCAAGTGGATTATGTTCAATAAATGACTTGACAATAACCAATGTGGATGTTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

739

Amino Acids

83.52

Weight (kDa)

5.48

Isoelectric Point (pI)

40.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 69 - 178 5.6e-35 D-mannose binding lectin
S_locus_glycop PF00954 222 - 308 6.5e-19 S-locus glycoprotein domain
PAN_2 PF08276 331 - 398 9.7e-16 PAN-like domain
PK_Tyr_Ser-Thr PF07714 444 - 689 6.2e-43 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 444 - 689 7e-38 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 848
AccB7I CCANNNNNTGG 2 cut(s) 66, 208
AciI CCGC 3 cut(s) 160, 1463, 1860
AclI AACGTT 1 cut(s) 312
AclWI GGATC 4 cut(s) 641, 1650, 1844, 1899
AcsI RAATTY 4 cut(s) 171, 725, 1388, 1689
AcuI CTGAAG 2 cut(s) 121, 1353
AfaI GTAC 1 cut(s) 859
AfeI AGCGCT 1 cut(s) 1565
AfiI CCNNNNNNNGG 4 cut(s) 66, 208, 554, 1735
AflIII ACRYGT 2 cut(s) 860, 1225
AhlI ACTAGT 1 cut(s) 283
AjnI CCWGG 6 cut(s) 405, 472, 547, 649, 904, 1611
AjuI GAANNNNNNNTTGG 2 cut(s) 1493, 1525
AloI GAACNNNNNNTCC 4 cut(s) 381, 413, 1203, 1235
AluBI AGCT 8 cut(s) 120, 156, 426, 1034, 1183, 1509, 1830, 1946
AluI AGCT 8 cut(s) 120, 156, 426, 1034, 1183, 1509, 1830, 1946
Alw26I GTCTC 2 cut(s) 192, 342
AlwI GGATC 4 cut(s) 641, 1650, 1844, 1899
AlwNI CAGNNNCTG 1 cut(s) 2066
Ama87I CYCGRG 2 cut(s) 151, 1618
Aor51HI AGCGCT 1 cut(s) 1565
AoxI GGCC 2 cut(s) 653, 2048
ApeKI GCWGC 3 cut(s) 760, 809, 1183
ApoI RAATTY 4 cut(s) 171, 725, 1388, 1689
AseI ATTAAT 1 cut(s) 879
Asp700I GAANNNNTTC 2 cut(s) 1114, 1419
AspLEI GCGC 3 cut(s) 663, 1155, 1566
AspS9I GGNCC 4 cut(s) 653, 1483, 1542, 1726
AsuHPI GGTGA 9 cut(s) 104, 502, 794, 1039, 1190, 1601, 1770, 1946, 2096
AvaI CYCGRG 2 cut(s) 151, 1618
AvaII GGWCC 3 cut(s) 1483, 1542, 1726
BanI GGYRCC 1 cut(s) 848
BanII GRGCYC 1 cut(s) 2019
BauI CACGAG 1 cut(s) 1590
BbsI GAAGAC 1 cut(s) 1944
BbvI GCAGC 3 cut(s) 772, 796, 1170
BccI CCATC 9 cut(s) 83, 245, 404, 773, 795, 941, 998, 1202, 2161
BceAI ACGGC 2 cut(s) 606, 1140
BciT130I CCWGG 6 cut(s) 407, 474, 549, 651, 906, 1613
BciVI GTATCC 1 cut(s) 1060
BcoDI GTCTC 2 cut(s) 192, 342
BcuI ACTAGT 1 cut(s) 283
BfaI CTAG 6 cut(s) 101, 284, 630, 705, 1667, 1970
BfmI CTRYAG 3 cut(s) 456, 562, 853
BfoI RGCGCY 1 cut(s) 1567
BfuI GTATCC 1 cut(s) 1060
BisI GCNGC 4 cut(s) 761, 810, 1184, 1861
BlsI GCNGC 4 cut(s) 762, 811, 1185, 1862
Bme1390I CCNGG 6 cut(s) 407, 474, 549, 651, 906, 1613
Bme18I GGWCC 3 cut(s) 1483, 1542, 1726
BmeT110I CYCGRG 2 cut(s) 151, 1618
BmgT120I GGNCC 4 cut(s) 653, 1483, 1542, 1726
BmiI GGNNCC 4 cut(s) 584, 850, 1433, 1727
BmrFI CCNGG 6 cut(s) 407, 474, 549, 651, 906, 1613
BmrI ACTGGG 1 cut(s) 564
BmsI GCATC 5 cut(s) 1146, 1660, 1991, 2053, 2099
BmuI ACTGGG 1 cut(s) 564
BpiI GAAGAC 1 cut(s) 1944
BpmI CTGGAG 1 cut(s) 1020
BpuEI CTTGAG 1 cut(s) 2017
BsaJI CCNNGG 7 cut(s) 51, 405, 472, 547, 650, 656, 905
BsaXI ACNNNNNCTCC 4 cut(s) 66, 96, 1203, 1233
Bsc4I CCNNNNNNNGG 4 cut(s) 66, 208, 554, 1735
Bse118I RCCGGY 1 cut(s) 2046
Bse1I ACTGG 5 cut(s) 508, 559, 938, 1068, 1075
BseBI CCWGG 6 cut(s) 407, 474, 549, 651, 906, 1613
BseDI CCNNGG 7 cut(s) 51, 405, 472, 547, 650, 656, 905
BseGI GGATG 7 cut(s) 24, 94, 415, 1213, 1243, 2153, 2212
BseLI CCNNNNNNNGG 4 cut(s) 66, 208, 554, 1735
BseMII CTCAG 3 cut(s) 203, 956, 1665
BseNI ACTGG 5 cut(s) 508, 559, 938, 1068, 1075
BseXI GCAGC 3 cut(s) 772, 796, 1170
BsgI GTGCAG 2 cut(s) 354, 795
BshFI GGCC 2 cut(s) 655, 2050
BshNI GGYRCC 1 cut(s) 848
BsiHKCI CYCGRG 2 cut(s) 151, 1618
BsiSI CCGG 1 cut(s) 2047
BslFI GGGAC 2 cut(s) 572, 1739
BslI CCNNNNNNNGG 4 cut(s) 66, 208, 554, 1735
BsmAI GTCTC 2 cut(s) 192, 342
BsmFI GGGAC 2 cut(s) 572, 1739
BsmI GAATGC 1 cut(s) 2004
BsnI GGCC 2 cut(s) 655, 2050
BsoBI CYCGRG 2 cut(s) 151, 1618
Bsp1286I GDGCHC 1 cut(s) 2019
Bsp143I GATC 5 cut(s) 646, 1642, 1836, 1851, 1891
BspACI CCGC 3 cut(s) 160, 1463, 1860
BspANI GGCC 2 cut(s) 655, 2050
BspCNI CTCAG 3 cut(s) 202, 957, 1666
BspLI GGNNCC 4 cut(s) 584, 850, 1433, 1727
BspPI GGATC 4 cut(s) 641, 1650, 1844, 1899
BspT107I GGYRCC 1 cut(s) 848
BsrFI RCCGGY 1 cut(s) 2046
BsrI ACTGG 5 cut(s) 508, 559, 938, 1068, 1075
BssAI RCCGGY 1 cut(s) 2046
BssECI CCNNGG 7 cut(s) 51, 405, 472, 547, 650, 656, 905
BssMI GATC 5 cut(s) 646, 1642, 1836, 1851, 1891
BssSI CACGAG 1 cut(s) 1590
BssT1I CCWWGG 2 cut(s) 51, 656
Bst2BI CACGAG 1 cut(s) 1590
Bst2UI CCWGG 6 cut(s) 407, 474, 549, 651, 906, 1613
Bst4CI ACNGT 5 cut(s) 42, 208, 824, 843, 1051
Bst6I CTCTTC 1 cut(s) 1302
BstAPI GCANNNNNTGC 1 cut(s) 1161
BstC8I GCNNGC 3 cut(s) 1094, 2115, 2140
BstDEI CTNAG 4 cut(s) 189, 394, 965, 1674
BstEII GGTNACC 2 cut(s) 92, 782
BstF5I GGATG 7 cut(s) 24, 94, 415, 1213, 1243, 2153, 2212
BstH2I RGCGCY 1 cut(s) 1567
BstHHI GCGC 3 cut(s) 663, 1155, 1566
BstKTI GATC 5 cut(s) 649, 1645, 1839, 1854, 1894
BstMAI GTCTC 2 cut(s) 192, 342
BstMBI GATC 5 cut(s) 646, 1642, 1836, 1851, 1891
BstMWI GCNNNNNNNGC 1 cut(s) 1161
BstNI CCWGG 6 cut(s) 407, 474, 549, 651, 906, 1613
BstNSI RCATGY 4 cut(s) 864, 1131, 1229, 2142
BstPI GGTNACC 2 cut(s) 92, 782
BstSCI CCNGG 6 cut(s) 405, 472, 547, 649, 904, 1611
BstSFI CTRYAG 3 cut(s) 456, 562, 853
BstV1I GCAGC 3 cut(s) 772, 796, 1170
BstV2I GAAGAC 1 cut(s) 1944
BstX2I RGATCY 1 cut(s) 1642
BstYI RGATCY 1 cut(s) 1642
BsuI GTATCC 1 cut(s) 1060
BsuRI GGCC 2 cut(s) 655, 2050
BtsCI GGATG 7 cut(s) 24, 94, 415, 1213, 1243, 2153, 2212
BtsIMutI CAGTG 2 cut(s) 213, 848
Cac8I GCNNGC 3 cut(s) 1094, 2115, 2140
CaiI CAGNNNCTG 1 cut(s) 2066
CfoI GCGC 3 cut(s) 663, 1155, 1566
Cfr10I RCCGGY 1 cut(s) 2046
Cfr13I GGNCC 4 cut(s) 653, 1483, 1542, 1726
Csp6I GTAC 1 cut(s) 858
CviQI GTAC 1 cut(s) 858
DdeI CTNAG 4 cut(s) 189, 394, 965, 1674
DpnI GATC 5 cut(s) 648, 1644, 1838, 1853, 1893
DpnII GATC 5 cut(s) 646, 1642, 1836, 1851, 1891
Eam1104I CTCTTC 1 cut(s) 1302
EarI CTCTTC 1 cut(s) 1302
Eco130I CCWWGG 2 cut(s) 51, 656
Eco24I GRGCYC 1 cut(s) 2019
Eco32I GATATC 1 cut(s) 195
Eco47I GGWCC 3 cut(s) 1483, 1542, 1726
Eco47III AGCGCT 1 cut(s) 1565
Eco57I CTGAAG 2 cut(s) 121, 1353
Eco88I CYCGRG 2 cut(s) 151, 1618
Eco91I GGTNACC 2 cut(s) 92, 782
EcoO109I RGGNCCY 1 cut(s) 1542
EcoO65I GGTNACC 2 cut(s) 92, 782
EcoRI GAATTC 1 cut(s) 1388
EcoRII CCWGG 6 cut(s) 405, 472, 547, 649, 904, 1611
EcoRV GATATC 1 cut(s) 195
EcoT14I CCWWGG 2 cut(s) 51, 656
EcoT22I ATGCAT 1 cut(s) 2006
EcoT38I GRGCYC 1 cut(s) 2019
ErhI CCWWGG 2 cut(s) 51, 656
FalI AAGNNNNNCTT 6 cut(s) 1392, 1424, 1531, 1563, 2079, 2111
FaqI GGGAC 2 cut(s) 572, 1739
FauI CCCGC 1 cut(s) 1456
FauNDI CATATG 1 cut(s) 1765
Fnu4HI GCNGC 4 cut(s) 761, 810, 1184, 1861
FokI GGATG 6 cut(s) 11, 101, 422, 1220, 1250, 2140
FriOI GRGCYC 1 cut(s) 2019
Fsp4HI GCNGC 4 cut(s) 761, 810, 1184, 1861
FspBI CTAG 6 cut(s) 101, 284, 630, 705, 1667, 1970
GlaI GCGC 3 cut(s) 662, 1154, 1565
GluI GCNGC 4 cut(s) 761, 810, 1184, 1861
GsuI CTGGAG 1 cut(s) 1020
HaeII RGCGCY 1 cut(s) 1567
HaeIII GGCC 2 cut(s) 655, 2050
HapII CCGG 1 cut(s) 2047
HhaI GCGC 3 cut(s) 663, 1155, 1566
Hin6I GCGC 3 cut(s) 661, 1153, 1564
HinP1I GCGC 3 cut(s) 661, 1153, 1564
HindIII AAGCTT 2 cut(s) 424, 1507
HinfI GANTC 6 cut(s) 746, 937, 1597, 1616, 1961, 2036
HpaII CCGG 1 cut(s) 2047
HphI GGTGA 9 cut(s) 104, 502, 794, 1039, 1190, 1601, 1770, 1946, 2096
Hpy166II GTNNAC 2 cut(s) 45, 902
Hpy188I TCNGA 9 cut(s) 192, 199, 402, 942, 955, 966, 1696, 1819, 2062
Hpy188III TCNNGA 5 cut(s) 1037, 1620, 1667, 1834, 1996
Hpy8I GTNNAC 2 cut(s) 45, 902
HpyAV CCTTC 3 cut(s) 107, 790, 1533
HpyCH4III ACNGT 5 cut(s) 42, 208, 824, 843, 1051
HpyCH4IV ACGT 2 cut(s) 312, 689
HpyF10VI GCNNNNNNNGC 1 cut(s) 1161
HpyF3I CTNAG 4 cut(s) 189, 394, 965, 1674
HpySE526I ACGT 2 cut(s) 312, 689
HspAI GCGC 3 cut(s) 661, 1153, 1564
Kzo9I GATC 5 cut(s) 646, 1642, 1836, 1851, 1891
LmnI GCTCC 1 cut(s) 1039
Lsp1109I GCAGC 3 cut(s) 772, 796, 1170
LweI GCATC 5 cut(s) 1146, 1660, 1991, 2053, 2099
MaeI CTAG 6 cut(s) 101, 284, 630, 705, 1667, 1970
MaeII ACGT 2 cut(s) 312, 689
MaeIII GTNAC 8 cut(s) 3, 92, 534, 782, 827, 863, 1323, 1776
MalI GATC 5 cut(s) 648, 1644, 1838, 1853, 1893
MboI GATC 5 cut(s) 646, 1642, 1836, 1851, 1891
MboII GAAGA 8 cut(s) 127, 1319, 1397, 1481, 1598, 1655, 1949, 2045
MfeI CAATTG 1 cut(s) 1730
MflI RGATCY 1 cut(s) 1642
MhlI GDGCHC 1 cut(s) 2019
MlyI GAGTC 1 cut(s) 1610
MmeI TCCRAC 7 cut(s) 61, 148, 476, 958, 979, 1535, 1738
MnlI CCTC 8 cut(s) 38, 371, 949, 1009, 1205, 1264, 1555, 1882
Mph1103I ATGCAT 1 cut(s) 2006
MroXI GAANNNNTTC 2 cut(s) 1114, 1419
MseI TTAA 5 cut(s) 521, 623, 879, 1313, 1440
MslI CAYNNNNRTG 3 cut(s) 1206, 1230, 1262
MspI CCGG 1 cut(s) 2047
MspR9I CCNGG 6 cut(s) 407, 474, 549, 651, 906, 1613
MunI CAATTG 1 cut(s) 1730
Mva1269I GAATGC 1 cut(s) 2004
MvaI CCWGG 6 cut(s) 407, 474, 549, 651, 906, 1613
MwoI GCNNNNNNNGC 1 cut(s) 1161
NdeI CATATG 1 cut(s) 1765
NdeII GATC 5 cut(s) 646, 1642, 1836, 1851, 1891
NlaIV GGNNCC 4 cut(s) 584, 850, 1433, 1727
NmuCI GTSAC 5 cut(s) 92, 534, 782, 827, 1776
NsiI ATGCAT 1 cut(s) 2006
NspI RCATGY 4 cut(s) 864, 1131, 1229, 2142
PaeI GCATGC 1 cut(s) 2142
PaeR7I CTCGAG 2 cut(s) 151, 1618
PciI ACATGT 2 cut(s) 860, 1225
PctI GAATGC 1 cut(s) 2004
PdmI GAANNNNTTC 2 cut(s) 1114, 1419
PfeI GAWTC 5 cut(s) 746, 937, 1597, 1961, 2036
PflMI CCANNNNNTGG 2 cut(s) 66, 208
PkrI GCNGC 4 cut(s) 762, 811, 1185, 1862
PleI GAGTC 1 cut(s) 1610
PpsI GAGTC 1 cut(s) 1610
PpuMI RGGWCCY 1 cut(s) 1542
PscI ACATGT 2 cut(s) 860, 1225
PshBI ATTAAT 1 cut(s) 879
Psp1406I AACGTT 1 cut(s) 312
Psp5II RGGWCCY 1 cut(s) 1542
Psp6I CCWGG 6 cut(s) 405, 472, 547, 649, 904, 1611
PspEI GGTNACC 2 cut(s) 92, 782
PspGI CCWGG 6 cut(s) 405, 472, 547, 649, 904, 1611
PspN4I GGNNCC 4 cut(s) 584, 850, 1433, 1727
PspPI GGNCC 4 cut(s) 653, 1483, 1542, 1726
PspPPI RGGWCCY 1 cut(s) 1542
PspXI VCTCGAGB 1 cut(s) 151
PstNI CAGNNNCTG 1 cut(s) 2066
PsuI RGATCY 1 cut(s) 1642
RsaI GTAC 1 cut(s) 859
RsaNI GTAC 1 cut(s) 858
RseI CAYNNNNRTG 3 cut(s) 1206, 1230, 1262
SaqAI TTAA 5 cut(s) 521, 623, 879, 1313, 1440
SatI GCNGC 4 cut(s) 761, 810, 1184, 1861
Sau3AI GATC 5 cut(s) 646, 1642, 1836, 1851, 1891
Sau96I GGNCC 4 cut(s) 653, 1483, 1542, 1726
SchI GAGTC 1 cut(s) 1610
ScrFI CCNGG 6 cut(s) 407, 474, 549, 651, 906, 1613
SduI GDGCHC 1 cut(s) 2019
SfaNI GCATC 5 cut(s) 1146, 1660, 1991, 2053, 2099
SfcI CTRYAG 3 cut(s) 456, 562, 853
Sfr274I CTCGAG 2 cut(s) 151, 1618
SinI GGWCC 3 cut(s) 1483, 1542, 1726
SlaI CTCGAG 2 cut(s) 151, 1618
SmiMI CAYNNNNRTG 3 cut(s) 1206, 1230, 1262
SmlI CTYRAG 3 cut(s) 151, 1618, 1996
SmoI CTYRAG 3 cut(s) 151, 1618, 1996
SpeI ACTAGT 1 cut(s) 283
SphI GCATGC 1 cut(s) 2142
SsiI CCGC 3 cut(s) 160, 1463, 1860
SspI AATATT 5 cut(s) 605, 877, 1660, 1716, 1845
SspMI CTAG 6 cut(s) 101, 284, 630, 705, 1667, 1970
StyD4I CCNGG 6 cut(s) 405, 472, 547, 649, 904, 1611
StyI CCWWGG 2 cut(s) 51, 656
TaaI ACNGT 5 cut(s) 42, 208, 824, 843, 1051
TaiI ACGT 2 cut(s) 315, 692
TaqI TCGA 5 cut(s) 152, 1046, 1499, 1619, 2212
TaqII GACCGA 1 cut(s) 330
TatI WGTACW 1 cut(s) 857
TauI GCSGC 1 cut(s) 1863
TfiI GAWTC 5 cut(s) 746, 937, 1597, 1961, 2036
Tru1I TTAA 5 cut(s) 521, 623, 879, 1313, 1440
Tru9I TTAA 5 cut(s) 521, 623, 879, 1313, 1440
TscAI CASTG 2 cut(s) 213, 848
TseFI GTSAC 5 cut(s) 92, 534, 782, 827, 1776
TseI GCWGC 3 cut(s) 760, 809, 1183
Tsp45I GTSAC 5 cut(s) 92, 534, 782, 827, 1776
TspGWI ACGGA 2 cut(s) 87, 1220
TspRI CASTG 2 cut(s) 213, 848
Van91I CCANNNNNTGG 2 cut(s) 66, 208
VpaK11BI GGWCC 3 cut(s) 1483, 1542, 1726
VspI ATTAAT 1 cut(s) 879
XapI RAATTY 4 cut(s) 171, 725, 1388, 1689
XbaI TCTAGA 1 cut(s) 1666
XceI RCATGY 4 cut(s) 864, 1131, 1229, 2142
XhoI CTCGAG 2 cut(s) 151, 1618
XmnI GAANNNNTTC 2 cut(s) 1114, 1419
XspI CTAG 6 cut(s) 101, 284, 630, 705, 1667, 1970
Zsp2I ATGCAT 1 cut(s) 2006
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.