RLG00000031363

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000112
Physical Location & Seq
Reverse (-)
4414243 .. 4417423
3181 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031363

Sequence Viewer

Length: 2193 bp
ATGTTATTCAAAGCTCTGCTTCTCTTCCTCCTCTTCCGATTTTGCGAATCCAGGGACACCATAACATGGAACCAAGAACAACTGAAGGATGATGGTGGAGTTTTGGTGTCCAAAGAAAGCAAGTTTGAATTGGGTTTCTTCAGCCCCAGAAATTCTAGCTACCGGTATGTTGGAATCTGGTATTCTCAGACTAGAGTACCTAATAAAACAGTGGTGTGGGTTGCAAACAGGAACAATCCCATCAATGATACCTCTGGTGTGCTCACAATGAACAGGTATGGAGACCTAGTCCTTTATGCTTATAACATGGAGAGCAGTCCTATATGGTCCACAAATGTGTCGCGGTCGGTTCAAAATGTTAACACAAGCACTTTATCTGCACAGCTTTTAGATACAGGAAATCTCGTTGTGTTCCAGGATGATAGTAATGAAAACTTTTTATGGCAAAGTTTTGATCATCCGACAGATACTTTAATTCCAGGTATGAAAGTTGGGGTGAATTGGAAAACTGGGCAAGAATGGGTTTTAACATCTTGGAAGTCACAAGATGACCCTGGAACTGGGGACTATACCTTGAGGCTCTATCCAAATCAGAGTGCATCCCCCCAATTTTTTATGTACAAGGGTTCGAGTAAGTATTGGCGAAGTGATCCAGGGACAACGGGTTTGGTCACTAATCAAGAGGAAACTTATTATCCCATGAATGACACCAATGCAATCAAAAGAGCAACAGTGACTGATTCTGCGGTAGAAGGCCTTGCATGGGATGATGGTGGCCTTCAATGGAAGGTAGACTTCTCTGCACCGAAGTCCCGGTGTGACTGGTATGGAGAGTGCGGTGCCAACAGCAAATGTAGCCCTGACAATGTTATTATGTTTTGGTGTGATTGTTTACCAGGGTATGTGCCTAATTCTATAAGTGATTGGAATCATATAAATGGGTCAGGTGGATGTGTGAGTAATCGACTTGGTTTGTTGAAGTGTGGAGATGGAGACGGGTTTGTGAAGGTGGCAAGAGTTAAGTATCCTGACGCATCGATAGGAGCATTGTCAAAATCAGGTATGAGTGCCAAAGAGTGTGAGCAGGAGTGCCTAAGAACTTGTTCTTGCACCGCATATTTGAGCACTGAATATGAAGGGCTTGTTGATTGCTTGACATGGTATGATGACTTGATGGACATTAAAGTGTACACAGAGATTGGACAAGATCTCTACGTTCGTGTGAATGCGACCGTGTTAGGCTCTAAAAATCTCCTTTTTGTTCTGGCTATGTTAGGTTCTGGCATGGCTGGTCAGCTACCAAATGAACAGAAAGTTGCTGTGAAAAGATTGTCCCAAACTTCAGGACAAGGGATCGAAGAATTCAAAAATGAAGTTGCACTTATAGCAAGACTTCAACACAGGAATCTTGTGAAACTTTTAGGCTGTTGTATAAAGGGAGAAGAAAGGATGTTAGTGCTAGAATATATGCCTAACAAAAGCTTGAACTCCTTTCTTTTTGATAACACAAGACGGTCCTTCTTGGATTGGGAAAAGCGATTTGAAATTATCAACGGGATTGCTCGTGGGATTCTGTATCTTCACCAGGACTCAAGATTGAGGATCATCCATAGAGATCTGAAAACTAGCAATATTCTTCTAGATGCTGAGATGAACCCAAAAATTTCTGATTTTGGCATGGCTAGAATATTCCACGGAGATCAACTGCAAGATAAGACGAGCCGAATTGTTGGAACATATGGCTACATGTCACCAGAGTACGCAGTATTTGGAAGATTTTCCATGAAATCTGATGTATTTAGTTTTGGGATCATAATGTTGGAGATTGTAAGTGGCCGGAAAAACAATGATTCTGATCTGGAGGATCCTTCCATGAACTTGATAGGACGTGTTTGGGAGCTCTGGGGAGAAGGCAGAGCCTTAGATATTGTTGATTCGACACTGAAGTCATATCAGCCTAATGAAGTCATGAGATGCATACAAGTTGCGCTCTTGTGTGTACAAGAAGATTCAAAGGACCGACCTGCCATGTCAGCCATCATTTTCATGTTGAGTGGTGAAGCATCTCCTCCATTGCCTAAGCATCCTGCATTTGTTTACAGAAGAGATTCCGGCGTTGATGTTGATCCATTACTTCCAAATGGATCTTATTCTATAAATGACTTGACAATAACTACAATGGAAGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

731

Amino Acids

82.2

Weight (kDa)

5.3

Isoelectric Point (pI)

38.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 70 - 180 1.7e-36 D-mannose binding lectin
S_locus_glycop PF00954 225 - 304 1.3e-11 S-locus glycoprotein domain
PAN_2 PF08276 328 - 394 8.1e-17 PAN-like domain
Pkinase PF00069 434 - 679 2.1e-39 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 435 - 681 2.4e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 303
AasI GACNNNNNNGTC 1 cut(s) 1945
Acc36I ACCTGC 1 cut(s) 2032
AccB1I GGYRCC 1 cut(s) 839
AccB7I CCANNNNNTGG 1 cut(s) 66
AccI GTMKAC 1 cut(s) 792
AccII CGCG 1 cut(s) 343
AciI CCGC 4 cut(s) 343, 746, 837, 1113
AclWI GGATC 8 cut(s) 644, 1361, 1610, 1817, 1859, 1872, 2120, 2152
AcoI YGGCCR 1 cut(s) 1834
AcsI RAATTY 3 cut(s) 151, 1361, 1662
AcuI CTGAAG 4 cut(s) 104, 124, 1326, 1964
AfaI GTAC 5 cut(s) 198, 620, 1190, 1760, 2001
AfiI CCNNNNNNNGG 3 cut(s) 66, 560, 763
AflIII ACRYGT 2 cut(s) 1746, 1888
AgeI ACCGGT 1 cut(s) 162
AjiI CACGTC 1 cut(s) 1889
AjnI CCWGG 7 cut(s) 50, 414, 478, 553, 652, 895, 1584
AleI CACNNNNGTG 1 cut(s) 335
AluBI AGCT 7 cut(s) 14, 159, 385, 1297, 1482, 1900, 2186
AluI AGCT 7 cut(s) 14, 159, 385, 1297, 1482, 1900, 2186
Alw21I GWGCWC 3 cut(s) 264, 1127, 1902
Alw26I GTCTC 2 cut(s) 276, 987
AlwI GGATC 8 cut(s) 644, 1361, 1610, 1817, 1859, 1872, 2120, 2152
AlwNI CAGNNNCTG 1 cut(s) 737
AoxI GGCC 3 cut(s) 754, 775, 1834
ApoI RAATTY 3 cut(s) 151, 1361, 1662
ArsI GACNNNNNNTTYG 2 cut(s) 649, 681
AsiGI ACCGGT 1 cut(s) 162
Asp700I GAANNNNTTC 3 cut(s) 1102, 1777, 2107
AspLEI GCGC 1 cut(s) 1990
AspS9I GGNCC 3 cut(s) 327, 1515, 2017
AsuC2I CCSGG 1 cut(s) 814
AsuHPI GGTGA 4 cut(s) 508, 1574, 1743, 2069
AvaII GGWCC 3 cut(s) 327, 1515, 2017
BaeI ACNNNNGTAYC 2 cut(s) 180, 213
BamHI GGATCC 1 cut(s) 1864
BanI GGYRCC 1 cut(s) 839
BanII GRGCYC 1 cut(s) 1902
BauI CACGAG 1 cut(s) 1563
Bbv12I GWGCWC 3 cut(s) 264, 1127, 1902
BccI CCATC 6 cut(s) 86, 248, 764, 983, 1168, 2045
BciT130I CCWGG 7 cut(s) 52, 416, 480, 555, 654, 897, 1586
BciVI GTATCC 1 cut(s) 1035
BclI TGATCA 1 cut(s) 454
BcnI CCSGG 1 cut(s) 814
BcoDI GTCTC 2 cut(s) 276, 987
BfaI CTAG 7 cut(s) 156, 192, 287, 1460, 1626, 1640, 1683
BfuAI ACCTGC 1 cut(s) 2032
BfuI GTATCC 1 cut(s) 1035
BglII AGATCT 2 cut(s) 1207, 1615
Bme1390I CCNGG 8 cut(s) 52, 416, 480, 555, 654, 814, 897, 1586
Bme18I GGWCC 3 cut(s) 327, 1515, 2017
BmgBI CACGTC 1 cut(s) 1889
BmgT120I GGNCC 3 cut(s) 327, 1515, 2017
BmiI GGNNCC 3 cut(s) 71, 841, 1866
BmrFI CCNGG 8 cut(s) 52, 416, 480, 555, 654, 814, 897, 1586
BmrI ACTGGG 2 cut(s) 519, 570
BmsI GCATC 6 cut(s) 608, 1043, 1633, 1964, 2072, 2092
BmuI ACTGGG 2 cut(s) 519, 570
BpmI CTGGAG 1 cut(s) 1880
Bpu10I CCTNAGC 1 cut(s) 2079
BpuEI CTTGAG 2 cut(s) 595, 1576
BpuMI CCSGG 1 cut(s) 814
Bsa29I ATCGAT 1 cut(s) 1037
BsaBI GATNNNNATC 3 cut(s) 927, 1854, 2124
BsaI GGTCTC 1 cut(s) 276
BsaJI CCNNGG 5 cut(s) 51, 553, 653, 896, 1693
BsaWI WCCGGW 1 cut(s) 162
BsaXI ACNNNNNCTCC 4 cut(s) 90, 120, 273, 303
Bsc4I CCNNNNNNNGG 3 cut(s) 66, 560, 763
Bse118I RCCGGY 1 cut(s) 162
Bse1I ACTGG 3 cut(s) 514, 565, 827
Bse3DI GCAATG 1 cut(s) 2072
Bse8I GATNNNNATC 3 cut(s) 927, 1854, 2124
BseBI CCWGG 7 cut(s) 52, 416, 480, 555, 654, 897, 1586
BseCI ATCGAT 1 cut(s) 1037
BseDI CCNNGG 5 cut(s) 51, 553, 653, 896, 1693
BseGI GGATG 9 cut(s) 94, 424, 457, 599, 772, 956, 1455, 1605, 2083
BseJI GATNNNNATC 3 cut(s) 927, 1854, 2124
BseLI CCNNNNNNNGG 3 cut(s) 66, 560, 763
BseMI GCAATG 1 cut(s) 2072
BseMII CTCAG 2 cut(s) 200, 1638
BseNI ACTGG 3 cut(s) 514, 565, 827
BseRI GAGGAG 2 cut(s) 20, 2058
BsgI GTGCAG 2 cut(s) 363, 786
Bsh1236I CGCG 1 cut(s) 343
Bsh1285I CGRYCG 2 cut(s) 347, 1233
BshFI GGCC 3 cut(s) 756, 777, 1836
BshNI GGYRCC 1 cut(s) 839
BshTI ACCGGT 1 cut(s) 162
BshVI ATCGAT 1 cut(s) 1037
BsiEI CGRYCG 2 cut(s) 347, 1233
BsiHKAI GWGCWC 3 cut(s) 264, 1127, 1902
BsiSI CCGG 4 cut(s) 163, 814, 1837, 2112
BslFI GGGAC 5 cut(s) 68, 578, 670, 796, 1318
BslI CCNNNNNNNGG 3 cut(s) 66, 560, 763
BsmAI GTCTC 2 cut(s) 276, 987
BsmBI CGTCTC 1 cut(s) 987
BsmFI GGGAC 5 cut(s) 68, 578, 670, 796, 1318
BsmI GAATGC 1 cut(s) 1231
BsnI GGCC 3 cut(s) 756, 777, 1836
Bso31I GGTCTC 1 cut(s) 276
Bsp1286I GDGCHC 3 cut(s) 264, 1127, 1902
Bsp1407I TGTACA 3 cut(s) 618, 1188, 1999
BspACI CCGC 4 cut(s) 343, 746, 837, 1113
BspANI GGCC 3 cut(s) 756, 777, 1836
BspCNI CTCAG 2 cut(s) 199, 1639
BspDI ATCGAT 1 cut(s) 1037
BspFNI CGCG 1 cut(s) 343
BspHI TCATGA 1 cut(s) 1968
BspLI GGNNCC 3 cut(s) 71, 841, 1866
BspMI ACCTGC 1 cut(s) 2032
BspPI GGATC 8 cut(s) 644, 1361, 1610, 1817, 1859, 1872, 2120, 2152
BspT107I GGYRCC 1 cut(s) 839
BspTNI GGTCTC 1 cut(s) 276
BsrDI GCAATG 1 cut(s) 2072
BsrFI RCCGGY 1 cut(s) 162
BsrGI TGTACA 3 cut(s) 618, 1188, 1999
BsrI ACTGG 3 cut(s) 514, 565, 827
BssAI RCCGGY 1 cut(s) 162
BssECI CCNNGG 5 cut(s) 51, 553, 653, 896, 1693
BssSI CACGAG 1 cut(s) 1563
Bst2BI CACGAG 1 cut(s) 1563
Bst2UI CCWGG 7 cut(s) 52, 416, 480, 555, 654, 897, 1586
Bst4CI ACNGT 4 cut(s) 211, 733, 1234, 1515
Bst6I CTCTTC 3 cut(s) 29, 38, 2098
BstAUI TGTACA 3 cut(s) 618, 1188, 1999
BstDEI CTNAG 5 cut(s) 186, 1094, 1647, 1921, 2079
BstDSI CCRYGG 1 cut(s) 1693
BstF5I GGATG 9 cut(s) 94, 424, 457, 599, 772, 956, 1455, 1605, 2083
BstFNI CGCG 1 cut(s) 343
BstHHI GCGC 1 cut(s) 1990
BstMAI GTCTC 2 cut(s) 276, 987
BstMCI CGRYCG 2 cut(s) 347, 1233
BstMWI GCNNNNNNNGC 3 cut(s) 855, 1385, 2033
BstNI CCWGG 7 cut(s) 52, 416, 480, 555, 654, 897, 1586
BstNSI RCATGY 1 cut(s) 1750
BstSCI CCNGG 8 cut(s) 50, 414, 478, 553, 652, 812, 895, 1584
BstUI CGCG 1 cut(s) 343
BstX2I RGATCY 4 cut(s) 1207, 1615, 1864, 2144
BstYI RGATCY 4 cut(s) 1207, 1615, 1864, 2144
Bsu15I ATCGAT 1 cut(s) 1037
BsuI GTATCC 1 cut(s) 1035
BsuRI GGCC 3 cut(s) 756, 777, 1836
BsuTUI ATCGAT 1 cut(s) 1037
BtgI CCRYGG 1 cut(s) 1693
BtrI CACGTC 1 cut(s) 1889
BtsCI GGATG 9 cut(s) 94, 424, 457, 599, 772, 956, 1455, 1605, 2083
BtsIMutI CAGTG 4 cut(s) 216, 738, 1125, 1940
BveI ACCTGC 1 cut(s) 2032
CaiI CAGNNNCTG 1 cut(s) 737
CciI TCATGA 1 cut(s) 1968
CfoI GCGC 1 cut(s) 1990
Cfr10I RCCGGY 1 cut(s) 162
Cfr13I GGNCC 3 cut(s) 327, 1515, 2017
ClaI ATCGAT 1 cut(s) 1037
CseI GACGC 1 cut(s) 1040
Csp6I GTAC 5 cut(s) 197, 619, 1189, 1759, 2000
CspAI ACCGGT 1 cut(s) 162
CviQI GTAC 5 cut(s) 197, 619, 1189, 1759, 2000
DdeI CTNAG 5 cut(s) 186, 1094, 1647, 1921, 2079
DrdI GACNNNNNNGTC 1 cut(s) 1945
DseDI GACNNNNNNGTC 1 cut(s) 1945
EaeI YGGCCR 1 cut(s) 1834
Eam1104I CTCTTC 3 cut(s) 29, 38, 2098
EarI CTCTTC 3 cut(s) 29, 38, 2098
Ecl136II GAGCTC 1 cut(s) 1900
Eco147I AGGCCT 1 cut(s) 756
Eco24I GRGCYC 1 cut(s) 1902
Eco31I GGTCTC 1 cut(s) 276
Eco47I GGWCC 3 cut(s) 327, 1515, 2017
Eco53kI GAGCTC 1 cut(s) 1900
Eco57I CTGAAG 4 cut(s) 104, 124, 1326, 1964
EcoICRI GAGCTC 1 cut(s) 1900
EcoRI GAATTC 1 cut(s) 1361
EcoRII CCWGG 7 cut(s) 50, 414, 478, 553, 652, 895, 1584
EcoT22I ATGCAT 1 cut(s) 1979
EcoT38I GRGCYC 1 cut(s) 1902
Esp3I CGTCTC 1 cut(s) 987
FalI AAGNNNNNCTT 5 cut(s) 35, 779, 811, 1365, 1397
FaqI GGGAC 5 cut(s) 68, 578, 670, 796, 1318
FauNDI CATATG 1 cut(s) 1738
FbaI TGATCA 1 cut(s) 454
FblI GTMKAC 1 cut(s) 792
FokI GGATG 9 cut(s) 101, 431, 444, 586, 779, 963, 1462, 1592, 2070
FriOI GRGCYC 1 cut(s) 1902
FspBI CTAG 7 cut(s) 156, 192, 287, 1460, 1626, 1640, 1683
GlaI GCGC 1 cut(s) 1989
GsuI CTGGAG 1 cut(s) 1880
HaeIII GGCC 3 cut(s) 756, 777, 1836
HapII CCGG 4 cut(s) 163, 814, 1837, 2112
HgaI GACGC 1 cut(s) 1040
HhaI GCGC 1 cut(s) 1990
Hin6I GCGC 1 cut(s) 1988
HinP1I GCGC 1 cut(s) 1988
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HindIII AAGCTT 1 cut(s) 1480
HpaI GTTAAC 1 cut(s) 361
HpaII CCGG 4 cut(s) 163, 814, 1837, 2112
HphI GGTGA 4 cut(s) 508, 1574, 1743, 2069
Hpy166II GTNNAC 8 cut(s) 330, 361, 793, 893, 1189, 1191, 2000, 2098
Hpy188I TCNGA 8 cut(s) 38, 189, 462, 594, 1620, 1669, 1792, 1855
Hpy188III TCNNGA 7 cut(s) 680, 1028, 1344, 1593, 1640, 1859, 1969
Hpy8I GTNNAC 8 cut(s) 330, 361, 793, 893, 1189, 1191, 2000, 2098
HpyAV CCTTC 9 cut(s) 79, 746, 781, 788, 1000, 1130, 1528, 1878, 1904
HpyCH4III ACNGT 4 cut(s) 211, 733, 1234, 1515
HpyCH4IV ACGT 2 cut(s) 1215, 1888
HpyF10VI GCNNNNNNNGC 3 cut(s) 855, 1385, 2033
HpyF3I CTNAG 5 cut(s) 186, 1094, 1647, 1921, 2079
HpySE526I ACGT 2 cut(s) 1215, 1888
HspAI GCGC 1 cut(s) 1988
Ksp22I TGATCA 1 cut(s) 454
KspAI GTTAAC 1 cut(s) 361
LmnI GCTCC 2 cut(s) 1043, 1897
LweI GCATC 6 cut(s) 608, 1043, 1633, 1964, 2072, 2092
MaeI CTAG 7 cut(s) 156, 192, 287, 1460, 1626, 1640, 1683
MaeII ACGT 2 cut(s) 1215, 1888
MaeIII GTNAC 5 cut(s) 540, 670, 733, 818, 1749
MflI RGATCY 4 cut(s) 1207, 1615, 1864, 2144
MhlI GDGCHC 3 cut(s) 264, 1127, 1902
MlyI GAGTC 1 cut(s) 1583
MmeI TCCRAC 4 cut(s) 151, 485, 1711, 1800
MnlI CCTC 8 cut(s) 38, 41, 262, 570, 676, 1593, 1855, 2079
Mph1103I ATGCAT 1 cut(s) 1979
MroXI GAANNNNTTC 3 cut(s) 1102, 1777, 2107
MseI TTAA 5 cut(s) 360, 473, 527, 1020, 1182
MslI CAYNNNNRTG 4 cut(s) 335, 936, 1184, 2045
MspI CCGG 4 cut(s) 163, 814, 1837, 2112
MspR9I CCNGG 8 cut(s) 52, 416, 480, 555, 654, 814, 897, 1586
Mva1269I GAATGC 1 cut(s) 1231
MvaI CCWGG 7 cut(s) 52, 416, 480, 555, 654, 897, 1586
MvnI CGCG 1 cut(s) 343
MwoI GCNNNNNNNGC 3 cut(s) 855, 1385, 2033
NciI CCSGG 1 cut(s) 814
NdeI CATATG 1 cut(s) 1738
NlaIV GGNNCC 3 cut(s) 71, 841, 1866
NmuCI GTSAC 5 cut(s) 540, 670, 733, 818, 1749
NsiI ATGCAT 1 cut(s) 1979
NspI RCATGY 1 cut(s) 1750
OliI CACNNNNGTG 1 cut(s) 335
PagI TCATGA 1 cut(s) 1968
PceI AGGCCT 1 cut(s) 756
PciI ACATGT 1 cut(s) 1746
PctI GAATGC 1 cut(s) 1231
PdmI GAANNNNTTC 3 cut(s) 1102, 1777, 2107
PflFI GACNNNGTC 1 cut(s) 287
PflMI CCANNNNNTGG 1 cut(s) 66
PfoI TCCNGGA 1 cut(s) 414
PinAI ACCGGT 1 cut(s) 162
PleI GAGTC 1 cut(s) 1583
PpsI GAGTC 1 cut(s) 1583
PscI ACATGT 1 cut(s) 1746
PsiI TTATAA 1 cut(s) 303
Psp124BI GAGCTC 1 cut(s) 1902
Psp6I CCWGG 7 cut(s) 50, 414, 478, 553, 652, 895, 1584
PspGI CCWGG 7 cut(s) 50, 414, 478, 553, 652, 895, 1584
PspN4I GGNNCC 3 cut(s) 71, 841, 1866
PspPI GGNCC 3 cut(s) 327, 1515, 2017
PstNI CAGNNNCTG 1 cut(s) 737
PsuI RGATCY 4 cut(s) 1207, 1615, 1864, 2144
PsyI GACNNNGTC 1 cut(s) 287
RsaI GTAC 5 cut(s) 198, 620, 1190, 1760, 2001
RsaNI GTAC 5 cut(s) 197, 619, 1189, 1759, 2000
RseI CAYNNNNRTG 4 cut(s) 335, 936, 1184, 2045
SacI GAGCTC 1 cut(s) 1902
SaqAI TTAA 5 cut(s) 360, 473, 527, 1020, 1182
Sau96I GGNCC 3 cut(s) 327, 1515, 2017
SchI GAGTC 1 cut(s) 1583
ScrFI CCNGG 8 cut(s) 52, 416, 480, 555, 654, 814, 897, 1586
SduI GDGCHC 3 cut(s) 264, 1127, 1902
SfaNI GCATC 6 cut(s) 608, 1043, 1633, 1964, 2072, 2092
SinI GGWCC 3 cut(s) 327, 1515, 2017
SmiMI CAYNNNNRTG 4 cut(s) 335, 936, 1184, 2045
SmlI CTYRAG 2 cut(s) 574, 1591
SmoI CTYRAG 2 cut(s) 574, 1591
SseBI AGGCCT 1 cut(s) 756
SsiI CCGC 4 cut(s) 343, 746, 837, 1113
SspI AATATT 2 cut(s) 1633, 1689
SspMI CTAG 7 cut(s) 156, 192, 287, 1460, 1626, 1640, 1683
SstI GAGCTC 1 cut(s) 1902
StuI AGGCCT 1 cut(s) 756
StyD4I CCNGG 8 cut(s) 50, 414, 478, 553, 652, 812, 895, 1584
TaaI ACNGT 4 cut(s) 211, 733, 1234, 1515
TaiI ACGT 2 cut(s) 1218, 1891
TaqI TCGA 6 cut(s) 629, 964, 1037, 1356, 1937, 2188
TaqII GACCGA 1 cut(s) 2034
TatI WGTACW 3 cut(s) 618, 1188, 1999
Tru1I TTAA 5 cut(s) 360, 473, 527, 1020, 1182
Tru9I TTAA 5 cut(s) 360, 473, 527, 1020, 1182
TscAI CASTG 4 cut(s) 216, 738, 1132, 1947
TseFI GTSAC 5 cut(s) 540, 670, 733, 818, 1749
Tsp45I GTSAC 5 cut(s) 540, 670, 733, 818, 1749
TspGWI ACGGA 1 cut(s) 1710
TspRI CASTG 4 cut(s) 216, 738, 1132, 1947
Tth111I GACNNNGTC 1 cut(s) 287
Van91I CCANNNNNTGG 1 cut(s) 66
VpaK11BI GGWCC 3 cut(s) 327, 1515, 2017
XapI RAATTY 3 cut(s) 151, 1361, 1662
XbaI TCTAGA 1 cut(s) 1639
XceI RCATGY 1 cut(s) 1750
XmiI GTMKAC 1 cut(s) 792
XmnI GAANNNNTTC 3 cut(s) 1102, 1777, 2107
XspI CTAG 7 cut(s) 156, 192, 287, 1460, 1626, 1640, 1683
Zsp2I ATGCAT 1 cut(s) 1979
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.