MD05G1332100.v1.1

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000112
Physical Location & Seq
Reverse (-)
45576594 .. 45579502
2909 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1332100.v1.1.491

Sequence Viewer

Length: 2247 bp
ATGCAAATCAACCTGAAAAAAACAGTTGTTTGGGTGGCAAACAGGGACAAGCCCATCAACGATAGTTCTGGAGTTCTAGCAATTAACAGGTACGGAATTCTGGTTCTTTCTGCTGCTGGGAACATTTCCATTTGGTCCACGGATGCGTCTGTCCATGTCCAAACCAGAAGCACTTTTGTAGCTCAGCTTTTAGATTCCGGAAATTTAGTGGTTTTCCAAGAAAATAAAAGTGAGAGCTTTATTTGGCAGAGTTTTGATTACCCAACTGACACTTTACTTCCAGGAATGAAAGTTGGGTTCAATTGGAAAACTGGGTTGGAATGGAAATTGACATCTTGGAATTCCGAAAAGGACCCTGGAACCGGGGACTACAGTTTTAGGGTAGTTCCCAATTCAACTGCCACCCCTCTAATTGTTGTGTTTAAGGGTTCGAGCAAGTATTGGAGATCTGATCCCGGGCCGTGGCCGACTTTTGTGAGCAATGATGAGGAAATGTCCTATTATTTTGTTCCGAATGATAGCGACTCTGTAGCACGATCGGTGTTGAGGGATTATGGATTGTATCAACAACTGAAATGGAATGATGCTGATGATCATTGGGAGGAATATTGGGCTGCACCAAAATATCGGTGTGACAAGTACGGACAGTGTGGTGCTAACAGCAGATGTAGCCCTGACAATTTTAATGCGTTTCAGTGTGAGTGCTTACCTGGGTTTGAACCGAAATCTGTGAATGATTGGAATCGGAGAAATGGGTCAGATGGGTGCGTGAGTAAGCGAATTGGGGGGTTGAAATGTGAAAATGGGGACGGGTTCGTGACGGTTCAAAGAGTGAAAGATCCAGATGCATCGATAGAAGCACGGTTGGTCAGAAGCATGAGTGCTAAAGAGTGTGAGCAGGCGTGCTTAAACAATTGTTCGTGCACTGCGTATATGAGCATAGAGTGGGAAGGGCGGCTGGATTGCATGACATGGCAAGATGATTTGATGGACATTGTAGGGCGCACAGAGTTGGGACGAGATTTATATGTTCGCGTGGACAAAATTGAATTAGCTAAGCATACCAGTAAATCAAAAGGTTTTTTGAGACGGAGGGGTGCTCTGGCTATTCCAATAGTTTCGGTACTGCTGGCATTGGTACTAATCATTGTGTTTGCCTGCTGGCAGCAAAAGAGAAAGAGTAAAACAAGAGCACGCACAGACTATGCGGAGGTAGATGAACTTGAGGAAGCTAAGAGACACCCTGAGTCGCAATATTTCAGTCTGAGCACCATAATAGCAGCCACAGATAACTTCTCACCCATTAACAAACTCGGCCAAGGGGGTTTTGGCATTGTTTATAAGGGCCACTTGCCAAATGGTCAGATTATTGCTGTAAAAAGATTGTCAAGAACTTCAGGACAAGGGGTCGAAGAATTCAAAAATGAAGTTGTGTTGATAGCAAGACTTCAACACAGGAATCTGGTGAAACTTTTGGGCTGTTGTATAAATGGAGAAGAAAGGATATTAGTCCTGGAACACTTGCCAAACAAAAGTTTGGATTACTTTCTTTTTGATCACAGAAGAAGGTCCTTGTTGGATTGGGATAAGCGTTTTGAAATAATCAATGGGGTTGTTCGTGGGATTCTGTATCTTCATCAGGACTCAAGAGTTAGGATTATCCATAGGGATCTAAAAACCAGCAATATTCTTCTAGATGCTGAGATGAACCCGAAAATTTCAGATTTCGGCATGGCAAGAATATTCCAGGGGGACCAACTGCGGGATAAGACCAACAGAATTGTTGGAACATATGGCTATATGTCACCAGAGTATGCAGTGTTTGGGAGATTTTCAACAAAGTCGGATGTCTTTAGTTTCGGGATCATATTGTTGGAGATTGTAAGCGGCAAGAAAAACAACGGTTTATATCAGGAGGATCATTCCTTGAATTTGATAGGACATGTTTGGCAGCTGTGGAGAGAAGACAGAGCTTTAGAAGTTGTGGATTCGTCATTAGAGTCATATCAGTCCGAGGAAGTCATGAGATGCATTCAGGTTGGGCTTTTGTGTGTGCAAGAAGATTCAGCGGACCGCCCAACCATGGCAGCCATTGTTTTCATGTTGAGTGGCGAAACATCTCCTCCAGCTCCTAAGCAGCCTGCATTTGTCTTCAGAAAAAAATCTCGTAGTAATGCCGATCCATCAGTTTCGAACGAATCATGTTCTATAAACAACTTGACAATAACTAAATTTCAAGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

749

Amino Acids

84.77

Weight (kDa)

7.76

Isoelectric Point (pI)

45.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 8 - 113 6.7e-38 D-mannose binding lectin
S_locus_glycop PF00954 158 - 243 2.1e-17 S-locus glycoprotein domain
PAN_2 PF08276 266 - 332 2e-16 PAN-like domain
Pkinase PF00069 435 - 698 1.6e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 436 - 700 1.7e-45 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1341
AccII CGCG 1 cut(s) 1035
AccIII TCCGGA 1 cut(s) 197
AciI CCGC 6 cut(s) 955, 1208, 1762, 1887, 2069, 2074
AclWI GGATC 6 cut(s) 446, 833, 1677, 1871, 1926, 2174
AcoI YGGCCR 2 cut(s) 464, 1315
AcsI RAATTY 7 cut(s) 96, 202, 340, 1415, 1716, 1930, 2231
AcuI CTGAAG 2 cut(s) 1380, 2137
AfaI GTAC 4 cut(s) 92, 641, 1125, 1140
AfiI CCNNNNNNNGG 4 cut(s) 362, 462, 1762, 2083
AflIII ACRYGT 1 cut(s) 1942
AhdI GACNNNNNGTC 1 cut(s) 1406
AjnI CCWGG 5 cut(s) 280, 355, 709, 1512, 1746
AjuI GAANNNNNNNTTGG 2 cut(s) 299, 331
AloI GAACNNNNNNTCC 2 cut(s) 87, 119
AluBI AGCT 9 cut(s) 182, 187, 237, 1055, 1232, 1954, 1973, 2129, 2240
AluI AGCT 9 cut(s) 182, 187, 237, 1055, 1232, 1954, 1973, 2129, 2240
Alw21I GWGCWC 4 cut(s) 926, 1102, 1195, 1271
Alw26I GTCTC 2 cut(s) 1081, 1231
Alw44I GTGCAC 1 cut(s) 922
AlwI GGATC 6 cut(s) 446, 833, 1677, 1871, 1926, 2174
Ama87I CYCGRG 1 cut(s) 455
Aor13HI TCCGGA 1 cut(s) 197
AoxI GGCC 4 cut(s) 458, 464, 1315, 1345
ApaLI GTGCAC 1 cut(s) 922
ApeKI GCWGC 7 cut(s) 113, 614, 1165, 1280, 1951, 2087, 2137
ApoI RAATTY 7 cut(s) 96, 202, 340, 1415, 1716, 1930, 2231
AspLEI GCGC 1 cut(s) 1005
AspS9I GGNCC 7 cut(s) 135, 352, 458, 1345, 1569, 1753, 2071
AsuC2I CCSGG 3 cut(s) 364, 456, 457
AsuHPI GGTGA 3 cut(s) 1290, 1477, 1797
AsuII TTCGAA 1 cut(s) 2192
AvaI CYCGRG 1 cut(s) 455
AvaII GGWCC 5 cut(s) 135, 352, 1569, 1753, 2071
BaeGI GKGCMC 1 cut(s) 926
BbsI GAAGAC 2 cut(s) 1971, 2143
Bbv12I GWGCWC 4 cut(s) 926, 1102, 1195, 1271
BbvI GCAGC 7 cut(s) 100, 601, 1177, 1292, 1963, 2099, 2149
BccI CCATC 4 cut(s) 62, 755, 982, 2191
BceAI ACGGC 1 cut(s) 445
BciT130I CCWGG 5 cut(s) 282, 357, 711, 1514, 1748
BclI TGATCA 2 cut(s) 592, 1555
BcnI CCSGG 3 cut(s) 364, 456, 457
BcoDI GTCTC 2 cut(s) 1081, 1231
BfaI CTAG 2 cut(s) 77, 1694
BfmI CTRYAG 2 cut(s) 370, 528
BglII AGATCT 1 cut(s) 446
BisI GCNGC 9 cut(s) 114, 615, 956, 1166, 1281, 1888, 1952, 2088, 2138
BlpI GCTNAGC 2 cut(s) 183, 1056
BlsI GCNGC 9 cut(s) 115, 616, 957, 1167, 1282, 1889, 1953, 2089, 2139
Bme1390I CCNGG 8 cut(s) 282, 357, 364, 456, 457, 711, 1514, 1748
Bme18I GGWCC 5 cut(s) 135, 352, 1569, 1753, 2071
BmeRI GACNNNNNGTC 1 cut(s) 1406
BmeT110I CYCGRG 1 cut(s) 455
BmgT120I GGNCC 7 cut(s) 135, 352, 458, 1345, 1569, 1753, 2071
BmiI GGNNCC 3 cut(s) 354, 361, 1754
BmrFI CCNGG 8 cut(s) 282, 357, 364, 456, 457, 711, 1514, 1748
BmrI ACTGGG 1 cut(s) 321
BmsI GCATC 6 cut(s) 133, 574, 835, 857, 1687, 2018
BmuI ACTGGG 1 cut(s) 321
BpiI GAAGAC 2 cut(s) 1971, 2143
BplI GAGNNNNNCTC 2 cut(s) 1084, 1116
BpmI CTGGAG 2 cut(s) 90, 2109
Bpu10I CCTNAGC 1 cut(s) 2133
Bpu1102I GCTNAGC 2 cut(s) 183, 1056
Bpu14I TTCGAA 1 cut(s) 2192
BpuEI CTTGAG 2 cut(s) 1244, 1630
BpuMI CCSGG 3 cut(s) 364, 456, 457
Bsa29I ATCGAT 1 cut(s) 851
BsaBI GATNNNNATC 1 cut(s) 741
BsaWI WCCGGW 1 cut(s) 197
BsaXI ACNNNNNCTCC 2 cut(s) 2107, 2137
Bsc4I CCNNNNNNNGG 4 cut(s) 362, 462, 1762, 2083
Bse1I ACTGG 2 cut(s) 316, 1065
Bse3DI GCAATG 1 cut(s) 487
Bse8I GATNNNNATC 1 cut(s) 741
BseAI TCCGGA 1 cut(s) 197
BseBI CCWGG 5 cut(s) 282, 357, 711, 1514, 1748
BseCI ATCGAT 1 cut(s) 851
BseGI GGATG 2 cut(s) 148, 1852
BseJI GATNNNNATC 1 cut(s) 741
BseLI CCNNNNNNNGG 4 cut(s) 362, 462, 1762, 2083
BseMI GCAATG 1 cut(s) 487
BseMII CTCAG 4 cut(s) 197, 1236, 1256, 1692
BseNI ACTGG 2 cut(s) 316, 1065
BseRI GAGGAG 1 cut(s) 2112
BseSI GKGCMC 1 cut(s) 926
BseXI GCAGC 7 cut(s) 100, 601, 1177, 1292, 1963, 2099, 2149
BseYI CCCAGC 1 cut(s) 116
BsgI GTGCAG 1 cut(s) 600
Bsh1236I CGCG 1 cut(s) 1035
Bsh1285I CGRYCG 1 cut(s) 539
BshFI GGCC 4 cut(s) 460, 466, 1317, 1347
BshVI ATCGAT 1 cut(s) 851
BsiEI CGRYCG 1 cut(s) 539
BsiHKAI GWGCWC 4 cut(s) 926, 1102, 1195, 1271
BsiHKCI CYCGRG 1 cut(s) 455
BsiSI CCGG 3 cut(s) 198, 363, 456
BslFI GGGAC 5 cut(s) 59, 380, 821, 1029, 1766
BslI CCNNNNNNNGG 4 cut(s) 362, 462, 1762, 2083
BsmAI GTCTC 2 cut(s) 1081, 1231
BsmBI CGTCTC 1 cut(s) 1081
BsmFI GGGAC 5 cut(s) 59, 380, 821, 1029, 1766
BsmI GAATGC 1 cut(s) 2031
BsnI GGCC 4 cut(s) 460, 466, 1317, 1347
BsoBI CYCGRG 1 cut(s) 455
Bsp119I TTCGAA 1 cut(s) 2192
Bsp1286I GDGCHC 4 cut(s) 926, 1102, 1195, 1271
Bsp13I TCCGGA 1 cut(s) 197
Bsp1720I GCTNAGC 2 cut(s) 183, 1056
Bsp19I CCATGG 1 cut(s) 2082
BspACI CCGC 6 cut(s) 955, 1208, 1762, 1887, 2069, 2074
BspANI GGCC 4 cut(s) 460, 466, 1317, 1347
BspCNI CTCAG 4 cut(s) 196, 1237, 1257, 1693
BspDI ATCGAT 1 cut(s) 851
BspEI TCCGGA 1 cut(s) 197
BspFNI CGCG 1 cut(s) 1035
BspHI TCATGA 1 cut(s) 2022
BspLI GGNNCC 3 cut(s) 354, 361, 1754
BspPI GGATC 6 cut(s) 446, 833, 1677, 1871, 1926, 2174
BspT104I TTCGAA 1 cut(s) 2192
BsrDI GCAATG 1 cut(s) 487
BsrI ACTGG 2 cut(s) 316, 1065
BssT1I CCWWGG 2 cut(s) 1318, 2082
Bst2UI CCWGG 5 cut(s) 282, 357, 711, 1514, 1748
Bst4CI ACNGT 6 cut(s) 25, 374, 648, 823, 864, 1904
BstBI TTCGAA 1 cut(s) 2192
BstC8I GCNNGC 7 cut(s) 900, 904, 1131, 1159, 1163, 1195, 2142
BstDEI CTNAG 7 cut(s) 183, 1056, 1233, 1245, 1265, 1701, 2133
BstDSI CCRYGG 3 cut(s) 138, 461, 2082
BstF5I GGATG 2 cut(s) 148, 1852
BstFNI CGCG 1 cut(s) 1035
BstHHI GCGC 1 cut(s) 1005
BstMAI GTCTC 2 cut(s) 1081, 1231
BstMCI CGRYCG 1 cut(s) 539
BstMWI GCNNNNNNNGC 1 cut(s) 669
BstNI CCWGG 5 cut(s) 282, 357, 711, 1514, 1748
BstNSI RCATGY 1 cut(s) 1946
BstSCI CCNGG 8 cut(s) 280, 355, 362, 454, 455, 709, 1512, 1746
BstSFI CTRYAG 2 cut(s) 370, 528
BstSLI GKGCMC 1 cut(s) 926
BstUI CGCG 1 cut(s) 1035
BstV1I GCAGC 7 cut(s) 100, 601, 1177, 1292, 1963, 2099, 2149
BstV2I GAAGAC 2 cut(s) 1971, 2143
BstX2I RGATCY 3 cut(s) 446, 838, 1669
BstYI RGATCY 3 cut(s) 446, 838, 1669
Bsu15I ATCGAT 1 cut(s) 851
BsuRI GGCC 4 cut(s) 460, 466, 1317, 1347
BsuTUI ATCGAT 1 cut(s) 851
BtgI CCRYGG 3 cut(s) 138, 461, 2082
BtsCI GGATG 2 cut(s) 148, 1852
BtsI GCAGTG 2 cut(s) 924, 1824
BtsIMutI CAGTG 4 cut(s) 653, 701, 924, 1824
Cac8I GCNNGC 7 cut(s) 900, 904, 1131, 1159, 1163, 1195, 2142
CciI TCATGA 1 cut(s) 2022
CfoI GCGC 1 cut(s) 1005
Cfr13I GGNCC 7 cut(s) 135, 352, 458, 1345, 1569, 1753, 2071
Cfr9I CCCGGG 1 cut(s) 455
ClaI ATCGAT 1 cut(s) 851
CpoI CGGWCCG 1 cut(s) 2071
CseI GACGC 1 cut(s) 135
Csp6I GTAC 4 cut(s) 91, 640, 1124, 1139
CspI CGGWCCG 1 cut(s) 2071
CviQI GTAC 4 cut(s) 91, 640, 1124, 1139
DdeI CTNAG 7 cut(s) 183, 1056, 1233, 1245, 1265, 1701, 2133
DriI GACNNNNNGTC 1 cut(s) 1406
EaeI YGGCCR 2 cut(s) 464, 1315
Eam1105I GACNNNNNGTC 1 cut(s) 1406
Eco130I CCWWGG 2 cut(s) 1318, 2082
Eco47I GGWCC 5 cut(s) 135, 352, 1569, 1753, 2071
Eco57I CTGAAG 2 cut(s) 1380, 2137
Eco88I CYCGRG 1 cut(s) 455
EcoO109I RGGNCCY 2 cut(s) 352, 1569
EcoRI GAATTC 3 cut(s) 96, 340, 1415
EcoRII CCWGG 5 cut(s) 280, 355, 709, 1512, 1746
EcoT14I CCWWGG 2 cut(s) 1318, 2082
EcoT22I ATGCAT 2 cut(s) 850, 2033
ErhI CCWWGG 2 cut(s) 1318, 2082
Esp3I CGTCTC 1 cut(s) 1081
FalI AAGNNNNNCTT 2 cut(s) 1334, 1366
FaqI GGGAC 5 cut(s) 59, 380, 821, 1029, 1766
FauI CCCGC 1 cut(s) 1755
FauNDI CATATG 1 cut(s) 1792
FbaI TGATCA 2 cut(s) 592, 1555
Fnu4HI GCNGC 9 cut(s) 114, 615, 956, 1166, 1281, 1888, 1952, 2088, 2138
FokI GGATG 2 cut(s) 155, 1859
Fsp4HI GCNGC 9 cut(s) 114, 615, 956, 1166, 1281, 1888, 1952, 2088, 2138
FspBI CTAG 2 cut(s) 77, 1694
GlaI GCGC 1 cut(s) 1004
GluI GCNGC 9 cut(s) 114, 615, 956, 1166, 1281, 1888, 1952, 2088, 2138
GsaI CCCAGC 1 cut(s) 120
GsuI CTGGAG 2 cut(s) 90, 2109
HaeIII GGCC 4 cut(s) 460, 466, 1317, 1347
HapII CCGG 3 cut(s) 198, 363, 456
HgaI GACGC 1 cut(s) 135
HhaI GCGC 1 cut(s) 1005
Hin6I GCGC 1 cut(s) 1003
HinP1I GCGC 1 cut(s) 1003
HpaII CCGG 3 cut(s) 198, 363, 456
HphI GGTGA 3 cut(s) 1290, 1477, 1797
Hpy166II GTNNAC 3 cut(s) 138, 924, 1039
Hpy8I GTNNAC 3 cut(s) 138, 924, 1039
HpyAV CCTTC 2 cut(s) 944, 1560
HpyCH4III ACNGT 6 cut(s) 25, 374, 648, 823, 864, 1904
HpyCH4V TGCA 9 cut(s) 4, 617, 848, 924, 966, 1817, 2031, 2056, 2144
HpyF10VI GCNNNNNNNGC 1 cut(s) 669
HpyF3I CTNAG 7 cut(s) 183, 1056, 1233, 1245, 1265, 1701, 2133
HspAI GCGC 1 cut(s) 1003
Kpn2I TCCGGA 1 cut(s) 197
Ksp22I TGATCA 2 cut(s) 592, 1555
LmnI GCTCC 1 cut(s) 2134
Lsp1109I GCAGC 7 cut(s) 100, 601, 1177, 1292, 1963, 2099, 2149
LweI GCATC 6 cut(s) 133, 574, 835, 857, 1687, 2018
MaeI CTAG 2 cut(s) 77, 1694
MaeIII GTNAC 3 cut(s) 632, 817, 1803
MboII GAAGA 8 cut(s) 1424, 1508, 1575, 1625, 1682, 1976, 2072, 2143
MfeI CAATTG 2 cut(s) 301, 913
MflI RGATCY 3 cut(s) 446, 838, 1669
MhlI GDGCHC 4 cut(s) 926, 1102, 1195, 1271
MlyI GAGTC 4 cut(s) 518, 1256, 1637, 2009
MmeI TCCRAC 5 cut(s) 297, 1557, 1765, 1824, 1854
Mph1103I ATGCAT 2 cut(s) 850, 2033
MroI TCCGGA 1 cut(s) 197
MseI TTAA 5 cut(s) 84, 423, 684, 908, 1305
MspA1I CMGCKG 2 cut(s) 1954, 2069
MspI CCGG 3 cut(s) 198, 363, 456
MspR9I CCNGG 8 cut(s) 282, 357, 364, 456, 457, 711, 1514, 1748
MunI CAATTG 2 cut(s) 301, 913
Mva1269I GAATGC 1 cut(s) 2031
MvaI CCWGG 5 cut(s) 282, 357, 711, 1514, 1748
MvnI CGCG 1 cut(s) 1035
MwoI GCNNNNNNNGC 1 cut(s) 669
NciI CCSGG 3 cut(s) 364, 456, 457
NcoI CCATGG 1 cut(s) 2082
NdeI CATATG 1 cut(s) 1792
NlaIV GGNNCC 3 cut(s) 354, 361, 1754
NmeAIII GCCGAG 1 cut(s) 1293
NmuCI GTSAC 3 cut(s) 632, 817, 1803
NsiI ATGCAT 2 cut(s) 850, 2033
NspI RCATGY 1 cut(s) 1946
NspV TTCGAA 1 cut(s) 2192
PagI TCATGA 1 cut(s) 2022
PciI ACATGT 1 cut(s) 1942
PcsI WCGNNNNNNNCGW 1 cut(s) 926
PctI GAATGC 1 cut(s) 2031
PfeI GAWTC 7 cut(s) 194, 742, 1459, 1624, 1988, 2063, 2198
PfoI TCCNGGA 2 cut(s) 280, 1512
PkrI GCNGC 9 cut(s) 115, 616, 957, 1167, 1282, 1889, 1953, 2089, 2139
Ple19I CGATCG 1 cut(s) 539
PleI GAGTC 4 cut(s) 518, 1255, 1637, 2008
PpsI GAGTC 4 cut(s) 518, 1255, 1637, 2008
PpuMI RGGWCCY 2 cut(s) 352, 1569
PscI ACATGT 1 cut(s) 1942
PsiI TTATAA 1 cut(s) 1341
Psp5II RGGWCCY 2 cut(s) 352, 1569
Psp6I CCWGG 5 cut(s) 280, 355, 709, 1512, 1746
PspFI CCCAGC 1 cut(s) 116
PspGI CCWGG 5 cut(s) 280, 355, 709, 1512, 1746
PspN4I GGNNCC 3 cut(s) 354, 361, 1754
PspPI GGNCC 7 cut(s) 135, 352, 458, 1345, 1569, 1753, 2071
PspPPI RGGWCCY 2 cut(s) 352, 1569
PsuI RGATCY 3 cut(s) 446, 838, 1669
PvuI CGATCG 1 cut(s) 539
PvuII CAGCTG 1 cut(s) 1954
RsaI GTAC 4 cut(s) 92, 641, 1125, 1140
RsaNI GTAC 4 cut(s) 91, 640, 1124, 1139
Rsr2I CGGWCCG 1 cut(s) 2071
RsrII CGGWCCG 1 cut(s) 2071
SaqAI TTAA 5 cut(s) 84, 423, 684, 908, 1305
SatI GCNGC 9 cut(s) 114, 615, 956, 1166, 1281, 1888, 1952, 2088, 2138
Sau96I GGNCC 7 cut(s) 135, 352, 458, 1345, 1569, 1753, 2071
SchI GAGTC 4 cut(s) 518, 1256, 1637, 2009
ScrFI CCNGG 8 cut(s) 282, 357, 364, 456, 457, 711, 1514, 1748
SduI GDGCHC 4 cut(s) 926, 1102, 1195, 1271
SfaNI GCATC 6 cut(s) 133, 574, 835, 857, 1687, 2018
SfcI CTRYAG 2 cut(s) 370, 528
SfuI TTCGAA 1 cut(s) 2192
SinI GGWCC 5 cut(s) 135, 352, 1569, 1753, 2071
SmaI CCCGGG 1 cut(s) 457
SmlI CTYRAG 2 cut(s) 1223, 1645
SmoI CTYRAG 2 cut(s) 1223, 1645
SsiI CCGC 6 cut(s) 955, 1208, 1762, 1887, 2069, 2074
SspI AATATT 4 cut(s) 608, 1256, 1687, 1743
SspMI CTAG 2 cut(s) 77, 1694
StyD4I CCNGG 8 cut(s) 280, 355, 362, 454, 455, 709, 1512, 1746
StyI CCWWGG 2 cut(s) 1318, 2082
TaaI ACNGT 6 cut(s) 25, 374, 648, 823, 864, 1904
TaqI TCGA 5 cut(s) 431, 851, 1410, 2192, 2242
TauI GCSGC 2 cut(s) 958, 1890
TfiI GAWTC 7 cut(s) 194, 742, 1459, 1624, 1988, 2063, 2198
Tru1I TTAA 5 cut(s) 84, 423, 684, 908, 1305
Tru9I TTAA 5 cut(s) 84, 423, 684, 908, 1305
TscAI CASTG 4 cut(s) 653, 701, 931, 1824
TseFI GTSAC 3 cut(s) 632, 817, 1803
TseI GCWGC 7 cut(s) 113, 614, 1165, 1280, 1951, 2087, 2137
Tsp45I GTSAC 3 cut(s) 632, 817, 1803
TspDTI ATGAA 6 cut(s) 302, 1233, 1440, 1625, 1721, 2089
TspGWI ACGGA 4 cut(s) 108, 155, 657, 1105
TspMI CCCGGG 1 cut(s) 455
TspRI CASTG 4 cut(s) 653, 701, 931, 1824
VneI GTGCAC 1 cut(s) 922
VpaK11BI GGWCC 5 cut(s) 135, 352, 1569, 1753, 2071
XapI RAATTY 7 cut(s) 96, 202, 340, 1415, 1716, 1930, 2231
XbaI TCTAGA 1 cut(s) 1693
XceI RCATGY 1 cut(s) 1946
XcmI CCANNNNNNNNNTGG 2 cut(s) 1325, 1355
XmaI CCCGGG 1 cut(s) 455
XspI CTAG 2 cut(s) 77, 1694
Zsp2I ATGCAT 2 cut(s) 850, 2033
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.