divergent subfamily of APPLE domains
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 227 |
| AclWI | GGATC | 1 cut(s) | 42 |
| AcsI | RAATTY | 1 cut(s) | 168 |
| AcuI | CTGAAG | 1 cut(s) | 118 |
| AfaI | GTAC | 3 cut(s) | 13, 186, 249 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 233 |
| AgsI | TTSAA | 1 cut(s) | 106 |
| AjnI | CCWGG | 4 cut(s) | 139, 273, 350, 405 |
| AluBI | AGCT | 3 cut(s) | 321, 386, 394 |
| AluI | AGCT | 3 cut(s) | 321, 386, 394 |
| AlwI | GGATC | 1 cut(s) | 42 |
| ApoI | RAATTY | 1 cut(s) | 168 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 393 |
| AspS9I | GGNCC | 3 cut(s) | 65, 291, 380 |
| AsuHPI | GGTGA | 1 cut(s) | 310 |
| AvaII | GGWCC | 3 cut(s) | 65, 291, 380 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 84, 117 |
| BccI | CCATC | 1 cut(s) | 80 |
| BcgI | CGANNNNNNTGC | 1 cut(s) | 32 |
| BciT130I | CCWGG | 4 cut(s) | 141, 275, 352, 407 |
| BciVI | GTATCC | 1 cut(s) | 85 |
| BfaI | CTAG | 1 cut(s) | 150 |
| BfuI | GTATCC | 1 cut(s) | 85 |
| BisI | GCNGC | 1 cut(s) | 227 |
| BlsI | GCNGC | 1 cut(s) | 228 |
| BmcAI | AGTACT | 1 cut(s) | 13 |
| Bme1390I | CCNGG | 4 cut(s) | 141, 275, 352, 407 |
| Bme18I | GGWCC | 3 cut(s) | 65, 291, 380 |
| BmgT120I | GGNCC | 3 cut(s) | 65, 291, 380 |
| BmrFI | CCNGG | 4 cut(s) | 141, 275, 352, 407 |
| BmsI | GCATC | 1 cut(s) | 303 |
| BsaJI | CCNNGG | 2 cut(s) | 139, 294 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 233 |
| Bse1I | ACTGG | 3 cut(s) | 73, 197, 238 |
| BseBI | CCWGG | 4 cut(s) | 141, 275, 352, 407 |
| BseDI | CCNNGG | 2 cut(s) | 139, 294 |
| BseGI | GGATG | 2 cut(s) | 91, 402 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 233 |
| BseNI | ACTGG | 3 cut(s) | 73, 197, 238 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 233 |
| Bsp143I | GATC | 1 cut(s) | 47 |
| BspACI | CCGC | 1 cut(s) | 227 |
| BspPI | GGATC | 1 cut(s) | 42 |
| BsrI | ACTGG | 3 cut(s) | 73, 197, 238 |
| BssECI | CCNNGG | 2 cut(s) | 139, 294 |
| BssMI | GATC | 1 cut(s) | 47 |
| BssT1I | CCWWGG | 1 cut(s) | 294 |
| Bst2UI | CCWGG | 4 cut(s) | 141, 275, 352, 407 |
| Bst6I | CTCTTC | 1 cut(s) | 38 |
| BstF5I | GGATG | 2 cut(s) | 91, 402 |
| BstKTI | GATC | 1 cut(s) | 50 |
| BstMBI | GATC | 1 cut(s) | 47 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 135 |
| BstNI | CCWGG | 4 cut(s) | 141, 275, 352, 407 |
| BstSCI | CCNGG | 4 cut(s) | 139, 273, 350, 405 |
| BstX2I | RGATCY | 1 cut(s) | 47 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 204 |
| BstYI | RGATCY | 1 cut(s) | 47 |
| BsuI | GTATCC | 1 cut(s) | 85 |
| BtsCI | GGATG | 2 cut(s) | 91, 402 |
| BtsIMutI | CAGTG | 1 cut(s) | 204 |
| Cfr13I | GGNCC | 3 cut(s) | 65, 291, 380 |
| Csp6I | GTAC | 3 cut(s) | 12, 185, 248 |
| CviAII | CATG | 1 cut(s) | 420 |
| CviJI | RGCY | 7 cut(s) | 129, 138, 226, 273, 321, 386, 394 |
| CviKI_1 | RGCY | 7 cut(s) | 129, 138, 226, 273, 321, 386, 394 |
| CviQI | GTAC | 3 cut(s) | 12, 185, 248 |
| DpnI | GATC | 1 cut(s) | 49 |
| DpnII | GATC | 1 cut(s) | 47 |
| Eam1104I | CTCTTC | 1 cut(s) | 38 |
| EarI | CTCTTC | 1 cut(s) | 38 |
| Eco130I | CCWWGG | 1 cut(s) | 294 |
| Eco47I | GGWCC | 3 cut(s) | 65, 291, 380 |
| Eco57I | CTGAAG | 1 cut(s) | 118 |
| EcoRII | CCWGG | 4 cut(s) | 139, 273, 350, 405 |
| EcoT14I | CCWWGG | 1 cut(s) | 294 |
| ErhI | CCWWGG | 1 cut(s) | 294 |
| FaeI | CATG | 1 cut(s) | 423 |
| FaiI | YATR | 7 cut(s) | 78, 98, 162, 177, 205, 345, 421 |
| FatI | CATG | 1 cut(s) | 419 |
| Fnu4HI | GCNGC | 1 cut(s) | 227 |
| FokI | GGATG | 2 cut(s) | 98, 389 |
| Fsp4HI | GCNGC | 1 cut(s) | 227 |
| FspBI | CTAG | 1 cut(s) | 150 |
| GluI | GCNGC | 1 cut(s) | 227 |
| Hin1II | CATG | 1 cut(s) | 423 |
| HindIII | AAGCTT | 1 cut(s) | 319 |
| HphI | GGTGA | 1 cut(s) | 310 |
| Hpy166II | GTNNAC | 1 cut(s) | 248 |
| Hpy188I | TCNGA | 1 cut(s) | 9 |
| Hpy188III | TCNNGA | 1 cut(s) | 375 |
| Hpy8I | GTNNAC | 1 cut(s) | 248 |
| HpyAV | CCTTC | 1 cut(s) | 38 |
| HpyCH4V | TGCA | 2 cut(s) | 45, 316 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 135 |
| Hsp92II | CATG | 1 cut(s) | 423 |
| Kzo9I | GATC | 1 cut(s) | 47 |
| LweI | GCATC | 1 cut(s) | 303 |
| MaeI | CTAG | 1 cut(s) | 150 |
| MaeIII | GTNAC | 1 cut(s) | 412 |
| MalI | GATC | 1 cut(s) | 49 |
| MboI | GATC | 1 cut(s) | 47 |
| MboII | GAAGA | 4 cut(s) | 25, 124, 231, 322 |
| MflI | RGATCY | 1 cut(s) | 47 |
| MluCI | AATT | 1 cut(s) | 168 |
| MmeI | TCCRAC | 1 cut(s) | 145 |
| MnlI | CCTC | 2 cut(s) | 198, 371 |
| MroXI | GAANNNNTTC | 1 cut(s) | 393 |
| MseI | TTAA | 2 cut(s) | 369, 399 |
| MspA1I | CMGCKG | 1 cut(s) | 386 |
| MspR9I | CCNGG | 4 cut(s) | 141, 275, 352, 407 |
| MvaI | CCWGG | 4 cut(s) | 141, 275, 352, 407 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 135 |
| NdeII | GATC | 1 cut(s) | 47 |
| NlaIII | CATG | 1 cut(s) | 423 |
| NmuCI | GTSAC | 1 cut(s) | 412 |
| PdmI | GAANNNNTTC | 1 cut(s) | 393 |
| PfoI | TCCNGGA | 2 cut(s) | 350, 405 |
| PkrI | GCNGC | 1 cut(s) | 228 |
| Psp6I | CCWGG | 4 cut(s) | 139, 273, 350, 405 |
| PspGI | CCWGG | 4 cut(s) | 139, 273, 350, 405 |
| PspPI | GGNCC | 3 cut(s) | 65, 291, 380 |
| PsuI | RGATCY | 1 cut(s) | 47 |
| PvuII | CAGCTG | 1 cut(s) | 386 |
| RsaI | GTAC | 3 cut(s) | 13, 186, 249 |
| RsaNI | GTAC | 3 cut(s) | 12, 185, 248 |
| SaqAI | TTAA | 2 cut(s) | 369, 399 |
| SatI | GCNGC | 1 cut(s) | 227 |
| Sau3AI | GATC | 1 cut(s) | 47 |
| Sau96I | GGNCC | 3 cut(s) | 65, 291, 380 |
| ScaI | AGTACT | 1 cut(s) | 13 |
| ScrFI | CCNGG | 4 cut(s) | 141, 275, 352, 407 |
| SetI | ASST | 6 cut(s) | 190, 300, 323, 382, 388, 396 |
| SfaNI | GCATC | 1 cut(s) | 303 |
| SinI | GGWCC | 3 cut(s) | 65, 291, 380 |
| Sse9I | AATT | 1 cut(s) | 168 |
| SsiI | CCGC | 1 cut(s) | 227 |
| SspMI | CTAG | 1 cut(s) | 150 |
| StyD4I | CCNGG | 4 cut(s) | 139, 273, 350, 405 |
| StyI | CCWWGG | 1 cut(s) | 294 |
| TasI | AATT | 1 cut(s) | 168 |
| TatI | WGTACW | 2 cut(s) | 11, 247 |
| TauI | GCSGC | 1 cut(s) | 229 |
| Tru1I | TTAA | 2 cut(s) | 369, 399 |
| Tru9I | TTAA | 2 cut(s) | 369, 399 |
| TscAI | CASTG | 1 cut(s) | 204 |
| TseFI | GTSAC | 1 cut(s) | 412 |
| Tsp45I | GTSAC | 1 cut(s) | 412 |
| TspDTI | ATGAA | 1 cut(s) | 372 |
| TspRI | CASTG | 1 cut(s) | 204 |
| VpaK11BI | GGWCC | 3 cut(s) | 65, 291, 380 |
| XapI | RAATTY | 1 cut(s) | 168 |
| XmnI | GAANNNNTTC | 1 cut(s) | 393 |
| XspI | CTAG | 1 cut(s) | 150 |
| ZrmI | AGTACT | 1 cut(s) | 13 |