RchiOBHm_Chr5g0005131

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000112
Physical Location & Seq
Reverse (-)
3340902 .. 3344660
3759 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28636

Sequence Viewer

Length: 2436 bp
ATGTTACTGAAAGCTCTGCTTCTCATCCTCCTTTTCCAACTGTCCACTTCCAAGGACACCATAACATGGAACGAAGAGCAAGTAAAAGATGGTGACCTTTTAGTGTCCAAAGGAAACAACTTTGAATTGGGTTTCTTCAGCCCCAGAAACTCTAGCTACCGCTATGTTGGAATTTGGTATTCCATGTCTCAGCTATCTGACCAAACAGTGGTGTGGGTGGCAAACAGAAACAACCCCATCAATGATACATCAGGTGTTCTCACAATAAACAGGTATGGAAAACTAGTCATTTATGCTCACAATATGGAGAACATTCCTATATGGTCTGCAAATATGTCTCAGTCAGTCATAAACACAAACCCCTTATCTGCACAACTTTTAGATACAGGAAACTTAGTTCTGACCCAGGATGGTGAAAGTGAAAGCTTTATTGTATGGCAGAGTTTTGATTATCCTACAGATACTCTAATCCCTGGTATGAAACTTGGGGTGAGTTGGAAAACTGGTTTAGAGTGGTTTTTAACGTCTTGGAAGTCACAAGATGACCCTGGAACTGGGGACTACAGCCATAGGCTTTATTGGAATCAGACGGTTGTGCTACAATTTTTTCTGTTTAAGGGTTTAACTACTAGGGTTTGGAGAAGTGATCCTGGGCCTAATTGGCGCAGTTTTGTTAGTAATAATGATGAAACATATTACCTACAATATAGTGATAACTCTAGAAAATTTAGAATGGTTGTGAATGATTCTGGGTTATTGCAGCATTTGACATGGAATGATGGTGACCATCAATGGAAGGAACTATGGGCTGTACCGAAATACCGTTGTGACATGTATGGACAGTGTGGTGCCTATAGTAAATGTAACCCTGACAATATTAATATGTTTGAGTGCGAGTGTTTACCAGGGTATGAACCTAAATCTATAAATGACTGGAATCAGAATGATGGGTCAGATGGATGTCGGAGTAAGCGAGTTGGAGTGTCAAAGTGTGGAGTTGGAGATGGGTTTTTAGAGGTGGCAAGGGTGAAAGCTCCAGACACATCGACAGTAGTAGCACGACTGGATACTGGTATGAGTGCCACAGAGTGCAAGCAGGAGTGTTTGAGAAATTGTTCTTGCACAGCATGTATGAGCTTCAATAATAAAGGGCGCATTGATTGCTTGACATGGTATGGTGAGCTGCTGGATATTGTAGAGCACACGGATGGAGGACAAGTTCTATATGTTCGTGTGGATGAAAACGAGTTAGCTATGAATTCCAGAAAATCAGAAGGTTTTTTGGAAAGGAGGGGGATGCTGGCTATTCCAATATTATTGTCTGGTTTGGTGGTGTTGGCACTAATCCCTGTGTTTGGCTGTTGGTGGCTTAAGAAAAGGAGGAAAACAAGAGACTCTGCAGTGGCGGATGAACTTGGAAAAGCCAGGAAACACCCTGATCTGCAATTTTTCTCCCTGAGCACAATAATTGTAGCCACAGACAACTTCCATTTTGGAAATAAGCTTGGGGAAGGTGGTTTTGGATCTGTTTATAAGGGTCAGTTACCAAATGGACAGAAGATTGCTGTGAAAAGATTGTCCAAAACTTCGGGGCAAGGGATTGAAGAATTCAAAAATGAAGTTGTGCTTATAGAAAGGCTTCAACACAGGAACCTTGTGAAACTTTTGGGTTGTTGCATAGAGGGAGAAGAAAAGATGTTGGTCCTTGAATACTTGTTGAACAAAAGCTTGGACTTCTTTCTTTTTGATCACTCAAGAAGGTCCTCTTTGGATTGGAAAAAGCGCTTTGAAATTATCAATGGGATTGCTCGTGGGATTCTTTATCTTCACCAGGACTCGAGAGTTAGAATTATACATAGGGATCTAAAAACCAGCAATATTCTTCTAGATGCTGAGATGAACCCAAAAATTTCAGATTTTGGCATGGCAAGAATATTTTCTGGGGACCCATTGCAGGATAAGACAGCCAGAGTTGTTGGAACATATGGGTATATGTCACCAGAATATGCAGTTTTTGGAAGATATTCAACAAAATCAGATGTTTTTAGCTTCGGGATCATAATATTGGAGATCGTAAGTGGCAAGAAAAACTCTGGTTCTTATAAGGAGGATCATTCCATCAACTTGATAGAAAATGTTTGGCAGATGTGGGGTGAAGACAGAGCCTTAGAAGTTCTTGATTCATCACTAGAGTTATATCAACCTGATGAAGTCTTGAGATGCATTCAAGTTGGGCTCTTATGTGTGCAAGAAGATTCAAAGGACCGACCTACCATGTCAGATGCTGTTTTCATGTTGAGTGGTGAAGCATCTCTTTCAACTCCTAAGCAGCCTGCATTTGTTTTTAGAAAAAGTGCATGCAGTAATGGTCCATCCCTTGCAAATGGATTATGTTCAATAAATGACTTAACAATAACCAATGTGGATGTTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

811

Amino Acids

91.75

Weight (kDa)

6.05

Isoelectric Point (pI)

38.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 69 - 178 2.1e-35 D-mannose binding lectin
S_locus_glycop PF00954 223 - 308 9.5e-19 S-locus glycoprotein domain
PAN_2 PF08276 331 - 398 4.6e-15 PAN-like domain
Pkinase PF00069 496 - 762 1.1e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 497 - 762 3.7e-49 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1533, 2103
AccB1I GGYRCC 1 cut(s) 848
AccB7I CCANNNNNTGG 2 cut(s) 66, 208
AciI CCGC 2 cut(s) 160, 1406
AclWI GGATC 5 cut(s) 641, 1531, 1869, 2063, 2118
AcsI RAATTY 5 cut(s) 171, 725, 1258, 1607, 1908
AcuI CTGAAG 1 cut(s) 121
AdeI CACNNNGTG 1 cut(s) 1089
AfaI GTAC 1 cut(s) 813
AfeI AGCGCT 1 cut(s) 1784
AfiI CCNNNNNNNGG 5 cut(s) 66, 208, 554, 1355, 1954
AflII CTTAAG 1 cut(s) 1370
AflIII ACRYGT 1 cut(s) 831
AhlI ACTAGT 1 cut(s) 283
AjnI CCWGG 7 cut(s) 405, 472, 547, 649, 904, 1424, 1830
AjuI GAANNNNNNNTTGG 4 cut(s) 1503, 1535, 1712, 1744
AloI GAACNNNNNNTCC 4 cut(s) 381, 413, 1203, 1235
Alw21I GWGCWC 2 cut(s) 1203, 1463
Alw26I GTCTC 3 cut(s) 192, 342, 1386
AlwI GGATC 5 cut(s) 641, 1531, 1869, 2063, 2118
AlwNI CAGNNNCTG 1 cut(s) 2285
Ama87I CYCGRG 1 cut(s) 1837
Aor51HI AGCGCT 1 cut(s) 1784
AoxI GGCC 1 cut(s) 653
ApeKI GCWGC 3 cut(s) 760, 1183, 2329
ApoI RAATTY 5 cut(s) 171, 725, 1258, 1607, 1908
AseI ATTAAT 1 cut(s) 879
Asp700I GAANNNNTTC 4 cut(s) 1114, 1638, 1936, 2023
AspLEI GCGC 3 cut(s) 666, 1155, 1785
AspS9I GGNCC 6 cut(s) 653, 1702, 1761, 1945, 2263, 2369
AvaI CYCGRG 1 cut(s) 1837
AvaII GGWCC 5 cut(s) 1702, 1761, 1945, 2263, 2369
BanI GGYRCC 1 cut(s) 848
BanII GRGCYC 1 cut(s) 2238
BauI CACGAG 1 cut(s) 1809
BbsI GAAGAC 1 cut(s) 2163
Bbv12I GWGCWC 2 cut(s) 1203, 1463
BbvI GCAGC 3 cut(s) 772, 1170, 2341
BciT130I CCWGG 7 cut(s) 407, 474, 549, 651, 906, 1426, 1832
BciVI GTATCC 1 cut(s) 1060
BclI TGATCA 1 cut(s) 1747
BcoDI GTCTC 3 cut(s) 192, 342, 1386
BcuI ACTAGT 1 cut(s) 283
BfaI CTAG 6 cut(s) 153, 284, 630, 720, 1886, 2189
BfmI CTRYAG 4 cut(s) 456, 562, 853, 1398
BfoI RGCGCY 1 cut(s) 1786
BfrI CTTAAG 1 cut(s) 1370
BfuI GTATCC 1 cut(s) 1060
BglI GCCNNNNNGGC 1 cut(s) 661
BisI GCNGC 3 cut(s) 761, 1184, 2330
BlsI GCNGC 3 cut(s) 762, 1185, 2331
Bme1390I CCNGG 7 cut(s) 407, 474, 549, 651, 906, 1426, 1832
Bme18I GGWCC 5 cut(s) 1702, 1761, 1945, 2263, 2369
BmeT110I CYCGRG 1 cut(s) 1837
BmgT120I GGNCC 6 cut(s) 653, 1702, 1761, 1945, 2263, 2369
BmiI GGNNCC 4 cut(s) 850, 1652, 1946, 1947
BmrFI CCNGG 7 cut(s) 407, 474, 549, 651, 906, 1426, 1832
BmrI ACTGGG 1 cut(s) 564
BmsI GCATC 5 cut(s) 1287, 1879, 2210, 2272, 2318
BmuI ACTGGG 1 cut(s) 564
BpiI GAAGAC 1 cut(s) 2163
BpmI CTGGAG 1 cut(s) 1020
Bpu10I CCTNAGC 2 cut(s) 1457, 2325
BpuEI CTTGAG 2 cut(s) 1738, 2236
BsaJI CCNNGG 6 cut(s) 51, 405, 472, 547, 650, 905
BsaXI ACNNNNNCTCC 2 cut(s) 1203, 1233
Bsc4I CCNNNNNNNGG 5 cut(s) 66, 208, 554, 1355, 1954
Bse1I ACTGG 5 cut(s) 508, 559, 938, 1068, 1075
Bse3DI GCAATG 1 cut(s) 1949
BseBI CCWGG 7 cut(s) 407, 474, 549, 651, 906, 1426, 1832
BseDI CCNNGG 6 cut(s) 51, 405, 472, 547, 650, 905
BseGI GGATG 9 cut(s) 24, 415, 965, 1213, 1243, 1302, 1414, 2372, 2431
BseLI CCNNNNNNNGG 5 cut(s) 66, 208, 554, 1355, 1954
BseMI GCAATG 1 cut(s) 1949
BseMII CTCAG 4 cut(s) 203, 353, 1448, 1884
BseNI ACTGG 5 cut(s) 508, 559, 938, 1068, 1075
BseXI GCAGC 3 cut(s) 772, 1170, 2341
BsgI GTGCAG 1 cut(s) 354
BshFI GGCC 1 cut(s) 655
BshNI GGYRCC 1 cut(s) 848
BsiHKAI GWGCWC 2 cut(s) 1203, 1463
BsiHKCI CYCGRG 1 cut(s) 1837
BslFI GGGAC 2 cut(s) 572, 1958
BslI CCNNNNNNNGG 5 cut(s) 66, 208, 554, 1355, 1954
BsmAI GTCTC 3 cut(s) 192, 342, 1386
BsmFI GGGAC 2 cut(s) 572, 1958
BsmI GAATGC 1 cut(s) 2223
BsnI GGCC 1 cut(s) 655
BsoBI CYCGRG 1 cut(s) 1837
Bsp1286I GDGCHC 3 cut(s) 1203, 1463, 2238
Bsp143I GATC 8 cut(s) 646, 1438, 1523, 1747, 1861, 2055, 2070, 2110
BspACI CCGC 2 cut(s) 160, 1406
BspANI GGCC 1 cut(s) 655
BspCNI CTCAG 4 cut(s) 202, 352, 1449, 1885
BspLI GGNNCC 4 cut(s) 850, 1652, 1946, 1947
BspMAI CTGCAG 1 cut(s) 1402
BspPI GGATC 5 cut(s) 641, 1531, 1869, 2063, 2118
BspQI GCTCTTC 1 cut(s) 69
BspT107I GGYRCC 1 cut(s) 848
BspTI CTTAAG 1 cut(s) 1370
BsrDI GCAATG 1 cut(s) 1949
BsrI ACTGG 5 cut(s) 508, 559, 938, 1068, 1075
BssECI CCNNGG 6 cut(s) 51, 405, 472, 547, 650, 905
BssMI GATC 8 cut(s) 646, 1438, 1523, 1747, 1861, 2055, 2070, 2110
BssSI CACGAG 1 cut(s) 1809
BssT1I CCWWGG 1 cut(s) 51
Bst2BI CACGAG 1 cut(s) 1809
Bst2UI CCWGG 7 cut(s) 407, 474, 549, 651, 906, 1426, 1832
Bst4CI ACNGT 6 cut(s) 42, 208, 592, 824, 843, 1051
Bst6I CTCTTC 1 cut(s) 69
BstAFI CTTAAG 1 cut(s) 1370
BstAPI GCANNNNNTGC 1 cut(s) 1161
BstC8I GCNNGC 4 cut(s) 1094, 1302, 2334, 2359
BstDEI CTNAG 7 cut(s) 189, 339, 394, 1457, 1893, 2167, 2325
BstEII GGTNACC 2 cut(s) 92, 782
BstF5I GGATG 9 cut(s) 24, 415, 965, 1213, 1243, 1302, 1414, 2372, 2431
BstH2I RGCGCY 1 cut(s) 1786
BstHHI GCGC 3 cut(s) 666, 1155, 1785
BstKTI GATC 8 cut(s) 649, 1441, 1526, 1750, 1864, 2058, 2073, 2113
BstMAI GTCTC 3 cut(s) 192, 342, 1386
BstMBI GATC 8 cut(s) 646, 1438, 1523, 1747, 1861, 2055, 2070, 2110
BstMWI GCNNNNNNNGC 2 cut(s) 661, 1161
BstNI CCWGG 7 cut(s) 407, 474, 549, 651, 906, 1426, 1832
BstNSI RCATGY 3 cut(s) 835, 1131, 2361
BstPI GGTNACC 2 cut(s) 92, 782
BstSCI CCNGG 7 cut(s) 405, 472, 547, 649, 904, 1424, 1830
BstSFI CTRYAG 4 cut(s) 456, 562, 853, 1398
BstV1I GCAGC 3 cut(s) 772, 1170, 2341
BstV2I GAAGAC 1 cut(s) 2163
BstX2I RGATCY 2 cut(s) 1523, 1861
BstYI RGATCY 2 cut(s) 1523, 1861
BsuI GTATCC 1 cut(s) 1060
BsuRI GGCC 1 cut(s) 655
BtsCI GGATG 9 cut(s) 24, 415, 965, 1213, 1243, 1302, 1414, 2372, 2431
BtsI GCAGTG 1 cut(s) 1407
BtsIMutI CAGTG 3 cut(s) 213, 848, 1407
Cac8I GCNNGC 4 cut(s) 1094, 1302, 2334, 2359
CaiI CAGNNNCTG 1 cut(s) 2285
CfoI GCGC 3 cut(s) 666, 1155, 1785
Cfr13I GGNCC 6 cut(s) 653, 1702, 1761, 1945, 2263, 2369
Csp6I GTAC 1 cut(s) 812
CviQI GTAC 1 cut(s) 812
DdeI CTNAG 7 cut(s) 189, 339, 394, 1457, 1893, 2167, 2325
DpnI GATC 8 cut(s) 648, 1440, 1525, 1749, 1863, 2057, 2072, 2112
DpnII GATC 8 cut(s) 646, 1438, 1523, 1747, 1861, 2055, 2070, 2110
DraIII CACNNNGTG 1 cut(s) 1089
Eam1104I CTCTTC 1 cut(s) 69
EarI CTCTTC 1 cut(s) 69
EciI GGCGGA 1 cut(s) 1421
Eco130I CCWWGG 1 cut(s) 51
Eco24I GRGCYC 1 cut(s) 2238
Eco47I GGWCC 5 cut(s) 1702, 1761, 1945, 2263, 2369
Eco47III AGCGCT 1 cut(s) 1784
Eco57I CTGAAG 1 cut(s) 121
Eco88I CYCGRG 1 cut(s) 1837
Eco91I GGTNACC 2 cut(s) 92, 782
EcoO109I RGGNCCY 2 cut(s) 1761, 1945
EcoO65I GGTNACC 2 cut(s) 92, 782
EcoRI GAATTC 2 cut(s) 1258, 1607
EcoRII CCWGG 7 cut(s) 405, 472, 547, 649, 904, 1424, 1830
EcoT14I CCWWGG 1 cut(s) 51
EcoT22I ATGCAT 1 cut(s) 2225
EcoT38I GRGCYC 1 cut(s) 2238
ErhI CCWWGG 1 cut(s) 51
FalI AAGNNNNNCTT 7 cut(s) 35, 1611, 1643, 1750, 1782, 2298, 2330
FaqI GGGAC 2 cut(s) 572, 1958
FauNDI CATATG 1 cut(s) 1984
FbaI TGATCA 1 cut(s) 1747
Fnu4HI GCNGC 3 cut(s) 761, 1184, 2330
FokI GGATG 8 cut(s) 11, 422, 972, 1220, 1250, 1309, 1421, 2359
FriOI GRGCYC 1 cut(s) 2238
Fsp4HI GCNGC 3 cut(s) 761, 1184, 2330
FspBI CTAG 6 cut(s) 153, 284, 630, 720, 1886, 2189
GlaI GCGC 3 cut(s) 665, 1154, 1784
GluI GCNGC 3 cut(s) 761, 1184, 2330
GsuI CTGGAG 1 cut(s) 1020
HaeII RGCGCY 1 cut(s) 1786
HaeIII GGCC 1 cut(s) 655
HhaI GCGC 3 cut(s) 666, 1155, 1785
Hin6I GCGC 3 cut(s) 664, 1153, 1783
HinP1I GCGC 3 cut(s) 664, 1153, 1783
HindIII AAGCTT 3 cut(s) 424, 1502, 1726
HinfI GANTC 8 cut(s) 583, 746, 937, 1394, 1816, 1835, 2180, 2255
Hpy166II GTNNAC 2 cut(s) 45, 902
Hpy188III TCNNGA 9 cut(s) 720, 1037, 1263, 1755, 1839, 1886, 2053, 2177, 2215
Hpy8I GTNNAC 2 cut(s) 45, 902
HpyAV CCTTC 4 cut(s) 790, 1268, 1505, 1752
HpyCH4III ACNGT 6 cut(s) 42, 208, 592, 824, 843, 1051
HpyCH4IV ACGT 1 cut(s) 524
HpyF10VI GCNNNNNNNGC 2 cut(s) 661, 1161
HpyF3I CTNAG 7 cut(s) 189, 339, 394, 1457, 1893, 2167, 2325
HpySE526I ACGT 1 cut(s) 524
HspAI GCGC 3 cut(s) 664, 1153, 1783
KflI GGGWCCC 1 cut(s) 1945
Ksp22I TGATCA 1 cut(s) 1747
Kzo9I GATC 8 cut(s) 646, 1438, 1523, 1747, 1861, 2055, 2070, 2110
LguI GCTCTTC 1 cut(s) 69
LmnI GCTCC 1 cut(s) 1039
Lsp1109I GCAGC 3 cut(s) 772, 1170, 2341
LweI GCATC 5 cut(s) 1287, 1879, 2210, 2272, 2318
MaeI CTAG 6 cut(s) 153, 284, 630, 720, 1886, 2189
MaeII ACGT 1 cut(s) 524
MaeIII GTNAC 8 cut(s) 3, 92, 534, 782, 827, 863, 1542, 1995
MalI GATC 8 cut(s) 648, 1440, 1525, 1749, 1863, 2057, 2072, 2112
MboI GATC 8 cut(s) 646, 1438, 1523, 1747, 1861, 2055, 2070, 2110
MflI RGATCY 2 cut(s) 1523, 1861
MhlI GDGCHC 3 cut(s) 1203, 1463, 2238
MlyI GAGTC 2 cut(s) 1388, 1829
MmeI TCCRAC 7 cut(s) 61, 148, 476, 944, 958, 979, 1957
MnlI CCTC 8 cut(s) 38, 1009, 1205, 1284, 1374, 1675, 1774, 2101
Mph1103I ATGCAT 1 cut(s) 2225
MroXI GAANNNNTTC 4 cut(s) 1114, 1638, 1936, 2023
MseI TTAA 6 cut(s) 521, 615, 623, 879, 1371, 2408
MslI CAYNNNNRTG 1 cut(s) 1206
MspCI CTTAAG 1 cut(s) 1370
MspR9I CCNGG 7 cut(s) 407, 474, 549, 651, 906, 1426, 1832
Mva1269I GAATGC 1 cut(s) 2223
MvaI CCWGG 7 cut(s) 407, 474, 549, 651, 906, 1426, 1832
MwoI GCNNNNNNNGC 2 cut(s) 661, 1161
NdeI CATATG 1 cut(s) 1984
NdeII GATC 8 cut(s) 646, 1438, 1523, 1747, 1861, 2055, 2070, 2110
NlaIV GGNNCC 4 cut(s) 850, 1652, 1946, 1947
NmuCI GTSAC 5 cut(s) 92, 534, 782, 827, 1995
NsiI ATGCAT 1 cut(s) 2225
NspI RCATGY 3 cut(s) 835, 1131, 2361
PaeI GCATGC 1 cut(s) 2361
PaeR7I CTCGAG 1 cut(s) 1837
PciI ACATGT 1 cut(s) 831
PciSI GCTCTTC 1 cut(s) 69
PcsI WCGNNNNNNNCGW 1 cut(s) 970
PctI GAATGC 1 cut(s) 2223
PdmI GAANNNNTTC 4 cut(s) 1114, 1638, 1936, 2023
PfeI GAWTC 6 cut(s) 583, 746, 937, 1816, 2180, 2255
PflMI CCANNNNNTGG 2 cut(s) 66, 208
PkrI GCNGC 3 cut(s) 762, 1185, 2331
PleI GAGTC 2 cut(s) 1388, 1829
PpsI GAGTC 2 cut(s) 1388, 1829
PpuMI RGGWCCY 2 cut(s) 1761, 1945
PscI ACATGT 1 cut(s) 831
PshBI ATTAAT 1 cut(s) 879
PsiI TTATAA 2 cut(s) 1533, 2103
Psp5II RGGWCCY 2 cut(s) 1761, 1945
Psp6I CCWGG 7 cut(s) 405, 472, 547, 649, 904, 1424, 1830
PspEI GGTNACC 2 cut(s) 92, 782
PspGI CCWGG 7 cut(s) 405, 472, 547, 649, 904, 1424, 1830
PspN4I GGNNCC 4 cut(s) 850, 1652, 1946, 1947
PspPI GGNCC 6 cut(s) 653, 1702, 1761, 1945, 2263, 2369
PspPPI RGGWCCY 2 cut(s) 1761, 1945
PstI CTGCAG 1 cut(s) 1402
PstNI CAGNNNCTG 1 cut(s) 2285
PsuI RGATCY 2 cut(s) 1523, 1861
RsaI GTAC 1 cut(s) 813
RsaNI GTAC 1 cut(s) 812
RseI CAYNNNNRTG 1 cut(s) 1206
SapI GCTCTTC 1 cut(s) 69
SaqAI TTAA 6 cut(s) 521, 615, 623, 879, 1371, 2408
SatI GCNGC 3 cut(s) 761, 1184, 2330
Sau3AI GATC 8 cut(s) 646, 1438, 1523, 1747, 1861, 2055, 2070, 2110
Sau96I GGNCC 6 cut(s) 653, 1702, 1761, 1945, 2263, 2369
SchI GAGTC 2 cut(s) 1388, 1829
ScrFI CCNGG 7 cut(s) 407, 474, 549, 651, 906, 1426, 1832
SduI GDGCHC 3 cut(s) 1203, 1463, 2238
SfaNI GCATC 5 cut(s) 1287, 1879, 2210, 2272, 2318
SfcI CTRYAG 4 cut(s) 456, 562, 853, 1398
Sfr274I CTCGAG 1 cut(s) 1837
SinI GGWCC 5 cut(s) 1702, 1761, 1945, 2263, 2369
SlaI CTCGAG 1 cut(s) 1837
SmiMI CAYNNNNRTG 1 cut(s) 1206
SmlI CTYRAG 4 cut(s) 1370, 1753, 1837, 2215
SmoI CTYRAG 4 cut(s) 1370, 1753, 1837, 2215
SpeI ACTAGT 1 cut(s) 283
SphI GCATGC 1 cut(s) 2361
SsiI CCGC 2 cut(s) 160, 1406
SspI AATATT 5 cut(s) 877, 1314, 1879, 1935, 2064
SspMI CTAG 6 cut(s) 153, 284, 630, 720, 1886, 2189
StyD4I CCNGG 7 cut(s) 405, 472, 547, 649, 904, 1424, 1830
StyI CCWWGG 1 cut(s) 51
TaaI ACNGT 6 cut(s) 42, 208, 592, 824, 843, 1051
TaiI ACGT 1 cut(s) 527
TaqI TCGA 3 cut(s) 1046, 1838, 2431
TaqII GACCGA 1 cut(s) 2280
TfiI GAWTC 6 cut(s) 583, 746, 937, 1816, 2180, 2255
Tru1I TTAA 6 cut(s) 521, 615, 623, 879, 1371, 2408
Tru9I TTAA 6 cut(s) 521, 615, 623, 879, 1371, 2408
TscAI CASTG 3 cut(s) 213, 848, 1407
TseFI GTSAC 5 cut(s) 92, 534, 782, 827, 1995
TseI GCWGC 3 cut(s) 760, 1183, 2329
Tsp45I GTSAC 5 cut(s) 92, 534, 782, 827, 1995
TspGWI ACGGA 1 cut(s) 1220
TspRI CASTG 3 cut(s) 213, 848, 1407
Van91I CCANNNNNTGG 2 cut(s) 66, 208
Vha464I CTTAAG 1 cut(s) 1370
VpaK11BI GGWCC 5 cut(s) 1702, 1761, 1945, 2263, 2369
VspI ATTAAT 1 cut(s) 879
XapI RAATTY 5 cut(s) 171, 725, 1258, 1607, 1908
XbaI TCTAGA 2 cut(s) 719, 1885
XceI RCATGY 3 cut(s) 835, 1131, 2361
XhoI CTCGAG 1 cut(s) 1837
XmnI GAANNNNTTC 4 cut(s) 1114, 1638, 1936, 2023
XspI CTAG 6 cut(s) 153, 284, 630, 720, 1886, 2189
Zsp2I ATGCAT 1 cut(s) 2225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.