MD10G1307800.v1.1

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000112
Physical Location & Seq
Reverse (-)
39297620 .. 39300729
3110 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1307800.v1.1.491

Sequence Viewer

Length: 2439 bp
ATGCCCAGAAAAATGGTTTTCAAACTACTTTTGCATGTTGTCTTCCTTCTCCTCATCGTCCTCTTCCCATTCTGCACATCCATAGACACCATAGCATTCAACCAACAGGTCAAAGATGGTGACTTCATAGTGTCGAAAGAAGGCAACGTTGAACTGGGATTCTTCAGCCCCGGCAACTCCAGCTCCCGCTACGTCGGAATTTGGTTTGCTCAAAAATATGAAAAGGGGCAGAAGGTAGTGGTTTGGGTTGCAAACAGAAACAACCCCATCAATGATACCTCTGGTGTGCTCACAATTGACAGGCATGGAAATTTAGTTCTTTATGCTCTTGAGAATATTTCTATTTGGTCTGCTTACGTGTCGGTTCAAACCAGAAGCACTTCTGTGGCTCAGCTTTTGGATTCGGGAAACTTAGTGCTTTTCCAGGACAATAGAAGTGATAGCTTTATATGGCAGAGTTTTGATTATCCAACTGACACTTTACTTCCAGGAATGAAAGTTGGGTTGAATTGGAAAAAAGGGTTGGAATGGATCTTAACATCTTGGAAATCAGAAGATGACCCTTCAACCGGGGACTATAGTTATCGGCTTTTTTCGAATCAGACTGCCATTCCACATTATTTTGTGTACAAGGGTTTAGCTAAGTATTGGAGATCTAATCCCGGGCCATGGCCTAATTATGTGAGCAATAGCGAAGAGAAGTATTATTTCCTTGTAAATGATAACACATCTGTAATTAGAACTGTGTTGAGGCACTATGGTTCATATCAACAACTGAGATGGAATGATGGTGATGATGAATGGGAGGAACTCTGGGAGGTGCCAAAATATCGGTGTGACAAGTATGGACAGTGTGGTCCTAACAGCAGATGTAGCCCTGAAAATATTTTGTTTGAGTGTGAGTGTCTTCCAGGGTATGAACCAAAATCTGTGAATAATTGGAACCGGAGAGATGGGTCGGATGGGTGCGTGAGTAAGCGAATTGGGGTGTCAAAGTGTGGGAATGGAGATGAGTTTGTAAAGGTGGTGCGTGTTAAAGATCCAGACACGTCGAGAGCAGCACAGTTGTTAACAAATATCAGTGCAAATGAGTGTGAGCAAGTGTGCTTGAGCAATTGTTCCTGCACAGCATATCTGAGAGCAGAATGGGAAGGGCGGAGTGATTGCTTGTTTTGGTATGGTGATTTGATGGACATCTTAGTGCGGACAGAGTTGGGACGAGATATATATGTTCGTGTGGACAAAGTCGAATTAGATCAGAATACTAGAAAATCGAAAGGTTTTCTGAGACGGAGGGGTTTGCTTGCTATTCCAATTGTTTTTGTTCTTATGGCATTTGTACTAATCATTGTGTTTGCCTGCTGGAAGCTTAAGAAAAAGAGGAAGACAGAAGACTTCGTGGGGGCAGATGAACTTGAAGCAACTAGGAGACACCCCGAGTTGCAACTTTTCGATCTGAGCACTATAATAGCAGCCACAGACAACTTTTCGCCCACCAGTGAACTTGGCCGAGGTGGTTTTGGGACTGTTTATAAGGGTCAGTTACCAAATGATCAGATTATTGCTATAAAAAGATTGTCAAGAACGTCAGGACAAGGGGTTGAAGAATTCAAAAATGAAGTTGCGCTGATAGCGAGACTTCAGCACAGGAATCTCGTGAAACTTTTAGGCTGTTGTATAAAGGGAGAAGAAAGGATGTTAGTCCTCGAACACTTGCCAAACAAAAGCTTGGACTCCTTTCTTTTTGATCACAGAAAAAGGTCCTTGTTGGATTGGAAAAAACGCTTTGAAATCATCAACGGGGTTGCTCGTGGAATTTTGTATCTTCATCAGGACTCAAGACTTAGAATTATCCATAGGGATCTAAAAACCAGCAACATTCTTCTAGATTCTGAGATGAACCCAAAAATTTCAGATTTCGGTATGGCAAGAATCTTCCATGGAGACCAACTGCAAGACAAAACCAACAGAGTTGTTGGAACATATGGCTATATGTCACCCGAGTACGTAGTGTTTGGGAGATTTTCAACCAAATCCGATGTTTTTAGTTTTGGGATCATATTATTGGAGATTATAAGCAGCAAGAAAAACAACGGTGTTTATCAGGATGATCATTCCATAAACTTGATAGGAAATGTTTGGCAGCTGTGGAGAGAAGGCAGACCCTTAGAAATTGTGGATTCGTCACTAGAGTCATTTCCGTCGGATGAAGTAATGCGATGCATTCAAGTTGGGCTCTTGTGCGTGGAAGAAGATTCGAAGGACCGACCATCAATGTCAACCGTTGTTTTCATGTTGAGTGGTGAAACATCTCCACCATCTCCTAAACAGCCTGCATTTGTCTTCAGAAAAAGTTCTTGTGATGATGCTGATCCATTAATTCCGAAAGGATCAGATTCTATGAACGGCTTGACAATAACTGCATTTGAAGCTCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

813

Amino Acids

92.65

Weight (kDa)

7.13

Isoelectric Point (pI)

43.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 80 - 183 6.8e-38 D-mannose binding lectin
S_locus_glycop PF00954 229 - 310 1.8e-17 S-locus glycoprotein domain
PAN_2 PF08276 333 - 399 1.8e-16 PAN-like domain
Pkinase PF00069 499 - 762 1.4e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 500 - 763 1e-47 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1533, 2075
AasI GACNNNNNNGTC 1 cut(s) 855
AccB1I GGYRCC 1 cut(s) 820
AciI CCGC 3 cut(s) 187, 1156, 1204
AclI AACGTT 1 cut(s) 147
AclWI GGATC 6 cut(s) 539, 1034, 1869, 2063, 2366, 2398
AcoI YGGCCR 1 cut(s) 1507
AcsI RAATTY 5 cut(s) 198, 310, 1607, 1815, 1908
AcuI CTGAAG 3 cut(s) 148, 1625, 2329
AfaI GTAC 3 cut(s) 629, 1341, 2006
AfiI CCNNNNNNNGG 2 cut(s) 569, 669
AflII CTTAAG 1 cut(s) 1370
AflIII ACRYGT 2 cut(s) 357, 1047
AjiI CACGTC 1 cut(s) 1050
AjnI CCWGG 3 cut(s) 423, 487, 910
AjuI GAANNNNNNNTTGG 2 cut(s) 506, 538
AleI CACNNNNGTG 1 cut(s) 383
AloI GAACNNNNNNTCC 2 cut(s) 300, 332
AluBI AGCT 8 cut(s) 183, 394, 444, 641, 1369, 1728, 2146, 2432
AluI AGCT 8 cut(s) 183, 394, 444, 641, 1369, 1728, 2146, 2432
Alw21I GWGCWC 2 cut(s) 291, 1463
Alw26I GTCTC 4 cut(s) 1282, 1423, 1630, 1938
AlwI GGATC 6 cut(s) 539, 1034, 1869, 2063, 2366, 2398
Ama87I CYCGRG 3 cut(s) 662, 1436, 2000
AoxI GGCC 3 cut(s) 665, 671, 1507
ApeKI GCWGC 4 cut(s) 1058, 1472, 2079, 2143
ApoI RAATTY 5 cut(s) 198, 310, 1607, 1815, 1908
AseI ATTAAT 1 cut(s) 2378
Asp700I GAANNNNTTC 2 cut(s) 379, 2353
AspLEI GCGC 1 cut(s) 1627
AspS9I GGNCC 4 cut(s) 665, 857, 1761, 2263
AsuC2I CCSGG 4 cut(s) 171, 571, 663, 664
AsuHPI GGTGA 5 cut(s) 131, 803, 1193, 1989, 2315
AsuII TTCGAA 2 cut(s) 596, 2258
AvaI CYCGRG 3 cut(s) 662, 1436, 2000
AvaII GGWCC 3 cut(s) 857, 1761, 2263
BanI GGYRCC 1 cut(s) 820
BanII GRGCYC 1 cut(s) 2238
BauI CACGAG 2 cut(s) 1655, 1809
BbsI GAAGAC 5 cut(s) 34, 899, 1391, 1398, 2335
Bbv12I GWGCWC 2 cut(s) 291, 1463
BbvI GCAGC 4 cut(s) 1070, 1484, 2091, 2155
BccI CCATC 9 cut(s) 110, 275, 774, 782, 947, 956, 1183, 2278, 2326
BceAI ACGGC 1 cut(s) 2422
BciT130I CCWGG 3 cut(s) 425, 489, 912
BclI TGATCA 3 cut(s) 1552, 1747, 2110
BcnI CCSGG 4 cut(s) 171, 571, 663, 664
BcoDI GTCTC 4 cut(s) 1282, 1423, 1630, 1938
BfaI CTAG 4 cut(s) 1266, 1425, 1886, 2189
BfmI CTRYAG 1 cut(s) 577
BfrI CTTAAG 1 cut(s) 1370
BglII AGATCT 1 cut(s) 653
BisI GCNGC 4 cut(s) 1059, 1473, 2080, 2144
BlpI GCTNAGC 1 cut(s) 390
BlsI GCNGC 4 cut(s) 1060, 1474, 2081, 2145
Bme1390I CCNGG 7 cut(s) 171, 425, 489, 571, 663, 664, 912
Bme18I GGWCC 3 cut(s) 857, 1761, 2263
BmeT110I CYCGRG 3 cut(s) 662, 1436, 2000
BmgBI CACGTC 1 cut(s) 1050
BmgT120I GGNCC 4 cut(s) 665, 857, 1761, 2263
BmiI GGNNCC 2 cut(s) 822, 944
BmrFI CCNGG 7 cut(s) 171, 425, 489, 571, 663, 664, 912
BmrI ACTGGG 1 cut(s) 164
BmsI GCATC 2 cut(s) 2210, 2356
BmuI ACTGGG 1 cut(s) 164
BpiI GAAGAC 5 cut(s) 34, 899, 1391, 1398, 2335
BpmI CTGGAG 1 cut(s) 163
Bpu1102I GCTNAGC 1 cut(s) 390
Bpu14I TTCGAA 2 cut(s) 596, 2258
BpuEI CTTGAG 3 cut(s) 350, 1129, 1822
BpuMI CCSGG 4 cut(s) 171, 571, 663, 664
BsaAI YACGTR 2 cut(s) 358, 2008
BsaBI GATNNNNATC 2 cut(s) 1193, 2370
BsaI GGTCTC 1 cut(s) 1938
BsaJI CCNNGG 7 cut(s) 169, 570, 662, 668, 911, 1510, 1939
BsaWI WCCGGW 1 cut(s) 945
BsaXI ACNNNNNCTCC 2 cut(s) 167, 197
Bsc4I CCNNNNNNNGG 2 cut(s) 569, 669
Bse1I ACTGG 2 cut(s) 159, 1497
Bse8I GATNNNNATC 2 cut(s) 1193, 2370
BseBI CCWGG 3 cut(s) 425, 489, 912
BseDI CCNNGG 7 cut(s) 169, 570, 662, 668, 911, 1510, 1939
BseGI GGATG 5 cut(s) 77, 967, 1701, 2113, 2212
BseJI GATNNNNATC 2 cut(s) 1193, 2370
BseLI CCNNNNNNNGG 2 cut(s) 569, 669
BseMII CTCAG 6 cut(s) 404, 767, 1127, 1277, 1448, 1884
BseNI ACTGG 2 cut(s) 159, 1497
BseRI GAGGAG 1 cut(s) 41
BseXI GCAGC 4 cut(s) 1070, 1484, 2091, 2155
BsgI GTGCAG 2 cut(s) 58, 1108
BshFI GGCC 3 cut(s) 667, 673, 1509
BshNI GGYRCC 1 cut(s) 820
BsiHKAI GWGCWC 2 cut(s) 291, 1463
BsiHKCI CYCGRG 3 cut(s) 662, 1436, 2000
BsiSI CCGG 4 cut(s) 171, 570, 663, 946
BslFI GGGAC 3 cut(s) 587, 1230, 1537
BslI CCNNNNNNNGG 2 cut(s) 569, 669
BsmAI GTCTC 4 cut(s) 1282, 1423, 1630, 1938
BsmBI CGTCTC 1 cut(s) 1282
BsmFI GGGAC 3 cut(s) 587, 1230, 1537
BsmI GAATGC 2 cut(s) 95, 2223
BsnI GGCC 3 cut(s) 667, 673, 1509
Bso31I GGTCTC 1 cut(s) 1938
BsoBI CYCGRG 3 cut(s) 662, 1436, 2000
Bsp119I TTCGAA 2 cut(s) 596, 2258
Bsp1286I GDGCHC 3 cut(s) 291, 1463, 2238
Bsp1407I TGTACA 1 cut(s) 627
Bsp1720I GCTNAGC 1 cut(s) 390
Bsp19I CCATGG 2 cut(s) 668, 1939
BspACI CCGC 3 cut(s) 187, 1156, 1204
BspANI GGCC 3 cut(s) 667, 673, 1509
BspCNI CTCAG 6 cut(s) 403, 768, 1128, 1278, 1449, 1885
BspLI GGNNCC 2 cut(s) 822, 944
BspPI GGATC 6 cut(s) 539, 1034, 1869, 2063, 2366, 2398
BspT104I TTCGAA 2 cut(s) 596, 2258
BspT107I GGYRCC 1 cut(s) 820
BspTI CTTAAG 1 cut(s) 1370
BspTNI GGTCTC 1 cut(s) 1938
BsrGI TGTACA 1 cut(s) 627
BsrI ACTGG 2 cut(s) 159, 1497
BssECI CCNNGG 7 cut(s) 169, 570, 662, 668, 911, 1510, 1939
BssSI CACGAG 2 cut(s) 1655, 1809
BssT1I CCWWGG 2 cut(s) 668, 1939
Bst2BI CACGAG 2 cut(s) 1655, 1809
Bst2UI CCWGG 3 cut(s) 425, 489, 912
Bst4CI ACNGT 6 cut(s) 745, 852, 1065, 1528, 2096, 2284
Bst6I CTCTTC 2 cut(s) 68, 690
BstAFI CTTAAG 1 cut(s) 1370
BstAUI TGTACA 1 cut(s) 627
BstBAI YACGTR 2 cut(s) 358, 2008
BstBI TTCGAA 2 cut(s) 596, 2258
BstC8I GCNNGC 3 cut(s) 1305, 1360, 2334
BstDSI CCRYGG 2 cut(s) 668, 1939
BstF5I GGATG 5 cut(s) 77, 967, 1701, 2113, 2212
BstHHI GCGC 1 cut(s) 1627
BstMAI GTCTC 4 cut(s) 1282, 1423, 1630, 1938
BstMWI GCNNNNNNNGC 4 cut(s) 180, 873, 1631, 2429
BstNI CCWGG 3 cut(s) 425, 489, 912
BstNSI RCATGY 1 cut(s) 38
BstSCI CCNGG 7 cut(s) 169, 423, 487, 569, 661, 662, 910
BstSFI CTRYAG 1 cut(s) 577
BstSNI TACGTA 1 cut(s) 2008
BstV1I GCAGC 4 cut(s) 1070, 1484, 2091, 2155
BstV2I GAAGAC 5 cut(s) 34, 899, 1391, 1398, 2335
BstX2I RGATCY 4 cut(s) 531, 653, 1039, 1861
BstXI CCANNNNNNTGG 1 cut(s) 13
BstYI RGATCY 4 cut(s) 531, 653, 1039, 1861
BsuRI GGCC 3 cut(s) 667, 673, 1509
BtgI CCRYGG 2 cut(s) 668, 1939
BtgZI GCGATG 1 cut(s) 2233
BtrI CACGTC 1 cut(s) 1050
BtsCI GGATG 5 cut(s) 77, 967, 1701, 2113, 2212
BtsIMutI CAGTG 3 cut(s) 857, 1087, 1504
Cac8I GCNNGC 3 cut(s) 1305, 1360, 2334
CfoI GCGC 1 cut(s) 1627
Cfr13I GGNCC 4 cut(s) 665, 857, 1761, 2263
Cfr9I CCCGGG 1 cut(s) 662
Csp6I GTAC 3 cut(s) 628, 1340, 2005
CviAII CATG 5 cut(s) 35, 305, 669, 1940, 2293
CviQI GTAC 3 cut(s) 628, 1340, 2005
DrdI GACNNNNNNGTC 1 cut(s) 855
DseDI GACNNNNNNGTC 1 cut(s) 855
EaeI YGGCCR 1 cut(s) 1507
Eam1104I CTCTTC 2 cut(s) 68, 690
EarI CTCTTC 2 cut(s) 68, 690
EciI GGCGGA 1 cut(s) 1171
Eco105I TACGTA 1 cut(s) 2008
Eco130I CCWWGG 2 cut(s) 668, 1939
Eco24I GRGCYC 1 cut(s) 2238
Eco31I GGTCTC 1 cut(s) 1938
Eco47I GGWCC 3 cut(s) 857, 1761, 2263
Eco57I CTGAAG 3 cut(s) 148, 1625, 2329
Eco88I CYCGRG 3 cut(s) 662, 1436, 2000
EcoO109I RGGNCCY 1 cut(s) 1761
EcoRI GAATTC 1 cut(s) 1607
EcoRII CCWGG 3 cut(s) 423, 487, 910
EcoT14I CCWWGG 2 cut(s) 668, 1939
EcoT22I ATGCAT 1 cut(s) 2225
EcoT38I GRGCYC 1 cut(s) 2238
ErhI CCWWGG 2 cut(s) 668, 1939
Esp3I CGTCTC 1 cut(s) 1282
FaeI CATG 5 cut(s) 38, 308, 672, 1943, 2296
FaqI GGGAC 3 cut(s) 587, 1230, 1537
FatI CATG 5 cut(s) 34, 304, 668, 1939, 2292
FauI CCCGC 1 cut(s) 194
FauNDI CATATG 1 cut(s) 1984
FbaI TGATCA 3 cut(s) 1552, 1747, 2110
Fnu4HI GCNGC 4 cut(s) 1059, 1473, 2080, 2144
FokI GGATG 5 cut(s) 64, 974, 1708, 2120, 2219
FriOI GRGCYC 1 cut(s) 2238
Fsp4HI GCNGC 4 cut(s) 1059, 1473, 2080, 2144
FspBI CTAG 4 cut(s) 1266, 1425, 1886, 2189
GlaI GCGC 1 cut(s) 1626
GluI GCNGC 4 cut(s) 1059, 1473, 2080, 2144
GsuI CTGGAG 1 cut(s) 163
HaeIII GGCC 3 cut(s) 667, 673, 1509
HapII CCGG 4 cut(s) 171, 570, 663, 946
HhaI GCGC 1 cut(s) 1627
Hin1II CATG 5 cut(s) 38, 308, 672, 1943, 2296
Hin6I GCGC 1 cut(s) 1625
HinP1I GCGC 1 cut(s) 1625
HincII GTYRAC 2 cut(s) 1071, 2280
HindII GTYRAC 2 cut(s) 1071, 2280
HindIII AAGCTT 2 cut(s) 1367, 1726
HpaI GTTAAC 1 cut(s) 1071
HpaII CCGG 4 cut(s) 171, 570, 663, 946
HphI GGTGA 5 cut(s) 131, 803, 1193, 1989, 2315
Hpy166II GTNNAC 5 cut(s) 628, 1071, 1240, 1502, 2280
Hpy8I GTNNAC 5 cut(s) 628, 1071, 1240, 1502, 2280
Hpy99I CGWCG 3 cut(s) 197, 1054, 2206
HpyAV CCTTC 7 cut(s) 56, 134, 226, 573, 1145, 2150, 2254
HpyCH4III ACNGT 6 cut(s) 745, 852, 1065, 1528, 2096, 2284
HpyCH4IV ACGT 6 cut(s) 147, 192, 357, 1049, 1586, 2007
HpyF10VI GCNNNNNNNGC 4 cut(s) 180, 873, 1631, 2429
HpySE526I ACGT 6 cut(s) 147, 192, 357, 1049, 1586, 2007
Hsp92II CATG 5 cut(s) 38, 308, 672, 1943, 2296
HspAI GCGC 1 cut(s) 1625
Ksp22I TGATCA 3 cut(s) 1552, 1747, 2110
KspAI GTTAAC 1 cut(s) 1071
LmnI GCTCC 1 cut(s) 188
Lsp1109I GCAGC 4 cut(s) 1070, 1484, 2091, 2155
LweI GCATC 2 cut(s) 2210, 2356
MaeI CTAG 4 cut(s) 1266, 1425, 1886, 2189
MaeII ACGT 6 cut(s) 147, 192, 357, 1049, 1586, 2007
MaeIII GTNAC 5 cut(s) 119, 836, 1542, 1995, 2184
MfeI CAATTG 3 cut(s) 294, 1114, 1314
MflI RGATCY 4 cut(s) 531, 653, 1039, 1861
MhlI GDGCHC 3 cut(s) 291, 1463, 2238
MlyI GAGTC 3 cut(s) 1727, 1829, 2201
MmeI TCCRAC 7 cut(s) 175, 494, 504, 939, 1749, 1957, 2184
Mph1103I ATGCAT 1 cut(s) 2225
MroXI GAANNNNTTC 2 cut(s) 379, 2353
MseI TTAA 5 cut(s) 536, 1035, 1070, 1371, 2378
MslI CAYNNNNRTG 2 cut(s) 383, 1199
MspA1I CMGCKG 1 cut(s) 2146
MspCI CTTAAG 1 cut(s) 1370
MspI CCGG 4 cut(s) 171, 570, 663, 946
MspR9I CCNGG 7 cut(s) 171, 425, 489, 571, 663, 664, 912
MunI CAATTG 3 cut(s) 294, 1114, 1314
Mva1269I GAATGC 2 cut(s) 95, 2223
MvaI CCWGG 3 cut(s) 425, 489, 912
MwoI GCNNNNNNNGC 4 cut(s) 180, 873, 1631, 2429
NciI CCSGG 4 cut(s) 171, 571, 663, 664
NcoI CCATGG 2 cut(s) 668, 1939
NdeI CATATG 1 cut(s) 1984
NlaIII CATG 5 cut(s) 38, 308, 672, 1943, 2296
NlaIV GGNNCC 2 cut(s) 822, 944
NmeAIII GCCGAG 1 cut(s) 1535
NmuCI GTSAC 4 cut(s) 119, 836, 1995, 2184
NsiI ATGCAT 1 cut(s) 2225
NspI RCATGY 1 cut(s) 38
NspV TTCGAA 2 cut(s) 596, 2258
OliI CACNNNNGTG 1 cut(s) 383
PctI GAATGC 2 cut(s) 95, 2223
PdmI GAANNNNTTC 2 cut(s) 379, 2353
PfeI GAWTC 9 cut(s) 159, 401, 598, 1651, 1889, 1932, 2180, 2255, 2396
PflFI GACNNNGTC 1 cut(s) 1244
PfoI TCCNGGA 2 cut(s) 423, 487
PkrI GCNGC 4 cut(s) 1060, 1474, 2081, 2145
PleI GAGTC 3 cut(s) 1727, 1829, 2200
PpsI GAGTC 3 cut(s) 1727, 1829, 2200
Ppu21I YACGTR 2 cut(s) 358, 2008
PpuMI RGGWCCY 1 cut(s) 1761
PshBI ATTAAT 1 cut(s) 2378
PsiI TTATAA 2 cut(s) 1533, 2075
Psp1406I AACGTT 1 cut(s) 147
Psp5II RGGWCCY 1 cut(s) 1761
Psp6I CCWGG 3 cut(s) 423, 487, 910
PspGI CCWGG 3 cut(s) 423, 487, 910
PspN4I GGNNCC 2 cut(s) 822, 944
PspPI GGNCC 4 cut(s) 665, 857, 1761, 2263
PspPPI RGGWCCY 1 cut(s) 1761
PsuI RGATCY 4 cut(s) 531, 653, 1039, 1861
PsyI GACNNNGTC 1 cut(s) 1244
PvuII CAGCTG 1 cut(s) 2146
RsaI GTAC 3 cut(s) 629, 1341, 2006
RsaNI GTAC 3 cut(s) 628, 1340, 2005
RseI CAYNNNNRTG 2 cut(s) 383, 1199
SaqAI TTAA 5 cut(s) 536, 1035, 1070, 1371, 2378
SatI GCNGC 4 cut(s) 1059, 1473, 2080, 2144
Sau96I GGNCC 4 cut(s) 665, 857, 1761, 2263
SchI GAGTC 3 cut(s) 1727, 1829, 2201
ScrFI CCNGG 7 cut(s) 171, 425, 489, 571, 663, 664, 912
SduI GDGCHC 3 cut(s) 291, 1463, 2238
SfaNI GCATC 2 cut(s) 2210, 2356
SfcI CTRYAG 1 cut(s) 577
SfuI TTCGAA 2 cut(s) 596, 2258
SinI GGWCC 3 cut(s) 857, 1761, 2263
SmaI CCCGGG 1 cut(s) 664
SmiMI CAYNNNNRTG 2 cut(s) 383, 1199
SmlI CTYRAG 4 cut(s) 329, 1108, 1370, 1837
SmoI CTYRAG 4 cut(s) 329, 1108, 1370, 1837
SnaBI TACGTA 1 cut(s) 2008
SsiI CCGC 3 cut(s) 187, 1156, 1204
SspI AATATT 2 cut(s) 337, 886
SspMI CTAG 4 cut(s) 1266, 1425, 1886, 2189
StyD4I CCNGG 7 cut(s) 169, 423, 487, 569, 661, 662, 910
StyI CCWWGG 2 cut(s) 668, 1939
TaaI ACNGT 6 cut(s) 745, 852, 1065, 1528, 2096, 2284
TaiI ACGT 6 cut(s) 150, 195, 360, 1052, 1589, 2010
TaqI TCGA 8 cut(s) 134, 596, 1052, 1248, 1274, 1452, 1707, 2258
TaqII GACCGA 1 cut(s) 2280
TatI WGTACW 2 cut(s) 627, 1339
TfiI GAWTC 9 cut(s) 159, 401, 598, 1651, 1889, 1932, 2180, 2255, 2396
Tru1I TTAA 5 cut(s) 536, 1035, 1070, 1371, 2378
Tru9I TTAA 5 cut(s) 536, 1035, 1070, 1371, 2378
TscAI CASTG 3 cut(s) 857, 1087, 1504
TseFI GTSAC 4 cut(s) 119, 836, 1995, 2184
TseI GCWGC 4 cut(s) 1058, 1472, 2079, 2143
Tsp45I GTSAC 4 cut(s) 119, 836, 1995, 2184
TspGWI ACGGA 2 cut(s) 1306, 2190
TspMI CCCGGG 1 cut(s) 662
TspRI CASTG 3 cut(s) 857, 1087, 1504
Tth111I GACNNNGTC 1 cut(s) 1244
Vha464I CTTAAG 1 cut(s) 1370
VpaK11BI GGWCC 3 cut(s) 857, 1761, 2263
VspI ATTAAT 1 cut(s) 2378
XapI RAATTY 5 cut(s) 198, 310, 1607, 1815, 1908
XbaI TCTAGA 1 cut(s) 1885
XceI RCATGY 1 cut(s) 38
XmaI CCCGGG 1 cut(s) 662
XmnI GAANNNNTTC 2 cut(s) 379, 2353
XspI CTAG 4 cut(s) 1266, 1425, 1886, 2189
Zsp2I ATGCAT 1 cut(s) 2225
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.