RchiOBHm_Chr3g0476801

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000112
Physical Location & Seq
Forward (+)
22762667 .. 22766096
3430 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44219

Sequence Viewer

Length: 2475 bp
ATGAAAAAGGCCCTCTTGGTCATTTTTTTATATTCACACATAGCCCTGAAAATGCTGTTGAAATCTCTGCTTCTCTTCCTCCTCTTCCAAGTTTGCACTTCCAGGGACACCTTGAGATCGAACGAAGAACTGAAGGATGATGATGGTGGACTTCTGGTGTCCAAAGAAAGCAGCTTTGAGTTGGGTTTCTTCAGCCCTGGAAATTCTAGCAACCGCTATGTTGGGATTTGGTATTCTCAATCTAAAGTATCTGAAAAAACAGTGGTGTGGATCGCAAACAGAGACAACCCCATCCATGATACCTCTGGAGTTTTCACCATAAACAGGTATGGAAACTTAGTTCTTTATGCTTATAACATGGAGACCAGTCCTATATGGTCTGCAGATGTGTCTCTTTCAGTCAAAAACACCAGCACCTTATCTGCATATGTACAGCTTTTGGATACAGGAAACTTAGTTATGTTCCAGAATGACAGAGACAGAATCTTTGTATGGCAAAGTTTCGATTTTCCTACAGATACTTTAATCCCTGGTATGAAAGTTGGGATGAATTGGAAAACTGGGTTAGAATGGGTTTTAACATCTTGGAAGTCACAAGATGACCCTGGAACTGGGGACTATACCTTTAGGCTTCATAATTGGAGCCACACTGTCACACCACAGTATTTTATTTATAAAGGTTTGACCAAGTATTGGCGAGGTGATACAGAGCCTTGGCCTAATTCGGTCCGAAATGCAGAGGAAATGTCTTTTTTCCTTACTTATAACACCAACAAACTTACAATAATGAAGTTGAAGTTTTCTGGGCTACATCAGCACTTAGAATGGAATGATGGTGACGATCAATGGAAGGAGTTGTTGTCTACACCGAAATACCGGTGTGACTGGTATGGACATTGTGGTGCCAACAGCAAATGTAGCCTTGACAGTGGTAATCTGTTCGAGTGTGAGTGTCTACCGGGGTATGAACCAAAATCCATAAATGGTTGGAATGGGAGAAATGGGTCAGATGGATGTGTGAGTAAACGAGTCAAAGTGTCAAAGTGTAGAAATGGAGAAGGGTTTGTGAAGGTGGAAAGGGTGAAAGATCCAGACGCATCGAGAGCAGCATTGTTGAAAAGAGGTATGAGTACCAAAGAGTGCGAGCAGGAGTGCCTAAGAAATTGTTCTTGCACGGCATATATGACCAAAAAGATTGCAGGGGTTGTTGATTGCTTGACATGGTATGATGACTTGATGGACATTTTGGTTTACACAGAGGCCAGACGAGATCTGTATGTTCGTGTGGATAAAATTGAGTTAGCTGAGAATGCCGCAAACTCACAAGGATTCTTCAGGAGGGGCATGCTTGTTGTTCCAATACTGTTTGCTCTGCTGGCCCTGGTACTAATAAATGTGCTTGCCTGTTGGTTGCTTAAGAAAAAAGGGAAGACAAAAGTCTTTGTGGAGGTGGATGAACTTGAGGAAAGCAAGAGATACCCGGAATTGCAATTTTTCGATCTGAACACACTAGTAGCAGCCACAGATAATTTCTCTGATGTTAATAAACTTGGCGAAGGTGGTTTTGGTGGTGTTTATAAGGGTGAGTTACCAAATGGACAGAAGGTTGCTGTAAAAAGGCTGTCTAGCAGCTCAGGACAAGGGATTGGTGAATTCAAAAATGAAGTTGAACTGATAGCAGGACTTCAACACAGGAATCTTGTGAAGCTTTTAGGTTGTTGTATAAAGGGAGGAGAAAGGATGCTAGTCCTAGAATATATGCCGAACAAAAGCTTGGACTCATTTCTTTTTGATGACACAAGAAGGTCCTTCTTAGATTGGGGAAGGCGTTTTGAGATTCTCAATGGGGTTGCTCGTGGGCTTTTGTATCTTCACCAAGACTCGAGATTAAGGATTATTCATAGGGATCTAAAAACCAGCAATATTCTTCTAGATGATGAGATGACCCCAAAAATCTCAGATTTCGGCATGGCAAGAATTTTCCATGGGGACCAACTTCAGGATAACACAAAGAAAGTTGTTGGAACATATGGCTACATGTCACCAGAGTATGCAGTGTTTGGGAGATTCTCTACGAAATCGGATGTGTTCAGTTTTGGGATTGTACTATTGGAGATTGTTAGTGGCAAGCGAAACAATGGTTCTTATGAGGAGAATTACTCCATGAACCTGATAGGACATGTTTGGCATGTGTGGAAAGAAGGAATAGCCTTAGATATTGTGGATTCATCGCTACAATCATATAAGCATGATGAAGTCTTGAGATGCATTCAAATTGGACTGTTGTGCGTGCAAGAAGATTCCAAGGACCGACCAGCCATGTCATCCGTTGTGTTCATGTTGAGTGGTGAGGCATCTCTTCCATCTCCTAAGCAGCCTGCATTTGTCTTCAGAAGAAATTCCAGCAGTGCTGCTGATTCAAAAGAATTGCTTTCTATTAACGACTTAACAATAACGATTGTGGGAGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

824

Amino Acids

93.41

Weight (kDa)

7.17

Isoelectric Point (pI)

39.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 87 - 197 1.3e-33 D-mannose binding lectin
S_locus_glycop PF00954 246 - 326 7.4e-15 S-locus glycoprotein domain
PAN_2 PF08276 349 - 415 7.2e-17 PAN-like domain
Pkinase PF00069 512 - 776 1.6e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 515 - 778 1.1e-47 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 784 - 824 1.2e-08 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 354, 675, 765, 1578
AccB1I GGYRCC 1 cut(s) 902
AccB7I CCANNNNNTGG 1 cut(s) 693
AccI GTMKAC 2 cut(s) 863, 955
AciI CCGC 2 cut(s) 214, 1314
AclWI GGATC 3 cut(s) 278, 1082, 1914
AcsI RAATTY 4 cut(s) 202, 1652, 1977, 2398
AcuI CTGAAG 5 cut(s) 152, 175, 1318, 1982, 2374
AfaI GTAC 4 cut(s) 432, 1132, 1386, 2106
AfiI CCNNNNNNNGG 4 cut(s) 324, 611, 693, 1999
AflII CTTAAG 1 cut(s) 1415
AflIII ACRYGT 2 cut(s) 2037, 2179
AgeI ACCGGT 1 cut(s) 876
AgsI TTSAA 8 cut(s) 61, 796, 1117, 1657, 1670, 1688, 2273, 2421
AhlI ACTAGT 1 cut(s) 1510
AjnI CCWGG 5 cut(s) 101, 196, 529, 604, 1380
AjuI GAANNNNNNNTTGG 4 cut(s) 1548, 1580, 1757, 1789
AloI GAACNNNNNNTCC 2 cut(s) 324, 356
AluBI AGCT 7 cut(s) 174, 436, 1304, 1632, 1708, 1773, 2468
AluI AGCT 7 cut(s) 174, 436, 1304, 1632, 1708, 1773, 2468
Alw21I GWGCWC 1 cut(s) 2470
Alw26I GTCTC 4 cut(s) 276, 356, 396, 471
AlwI GGATC 3 cut(s) 278, 1082, 1914
Ama87I CYCGRG 1 cut(s) 1882
AoxI GGCC 4 cut(s) 9, 716, 1260, 1377
ApeKI GCWGC 6 cut(s) 171, 1106, 1517, 1629, 2374, 2411
ApoI RAATTY 4 cut(s) 202, 1652, 1977, 2398
ArsI GACNNNNNNTTYG 2 cut(s) 470, 502
AsiGI ACCGGT 1 cut(s) 876
Asp700I GAANNNNTTC 2 cut(s) 1165, 2398
AspS9I GGNCC 6 cut(s) 10, 727, 1378, 1806, 1990, 2308
AsuC2I CCSGG 2 cut(s) 960, 1481
AsuHPI GGTGA 9 cut(s) 307, 713, 848, 1093, 1595, 1661, 1865, 2034, 2360
AvaI CYCGRG 1 cut(s) 1882
AvaII GGWCC 4 cut(s) 727, 1806, 1990, 2308
BanI GGYRCC 1 cut(s) 902
BanII GRGCYC 1 cut(s) 2470
BauI CACGAG 1 cut(s) 1854
BbsI GAAGAC 2 cut(s) 1436, 2380
Bbv12I GWGCWC 1 cut(s) 2470
BbvI GCAGC 6 cut(s) 183, 1118, 1529, 1641, 2386, 2398
BccI CCATC 6 cut(s) 137, 299, 827, 1004, 1231, 2371
BceAI ACGGC 1 cut(s) 1191
BciT130I CCWGG 5 cut(s) 103, 198, 531, 606, 1382
BciVI GTATCC 1 cut(s) 436
BcnI CCSGG 2 cut(s) 960, 1481
BcoDI GTCTC 4 cut(s) 276, 356, 396, 471
BcuI ACTAGT 1 cut(s) 1510
BfaI CTAG 6 cut(s) 207, 1511, 1626, 1745, 1751, 1931
BfmI CTRYAG 2 cut(s) 381, 513
BfrI CTTAAG 1 cut(s) 1415
BfuI GTATCC 1 cut(s) 436
BglII AGATCT 1 cut(s) 1270
BisI GCNGC 7 cut(s) 172, 1107, 1314, 1518, 1630, 2375, 2412
BlsI GCNGC 7 cut(s) 173, 1108, 1315, 1519, 1631, 2376, 2413
Bme1390I CCNGG 7 cut(s) 103, 198, 531, 606, 960, 1382, 1481
Bme18I GGWCC 4 cut(s) 727, 1806, 1990, 2308
BmeT110I CYCGRG 1 cut(s) 1882
BmgT120I GGNCC 6 cut(s) 10, 727, 1378, 1806, 1990, 2308
BmiI GGNNCC 3 cut(s) 644, 904, 1991
BmrFI CCNGG 7 cut(s) 103, 198, 531, 606, 960, 1382, 1481
BmrI ACTGGG 2 cut(s) 570, 621
BmsI GCATC 4 cut(s) 1106, 1731, 2255, 2363
BmuI ACTGGG 2 cut(s) 570, 621
BoxI GACNNNNGTC 1 cut(s) 1436
BpiI GAAGAC 2 cut(s) 1436, 2380
BplI GAGNNNNNCTC 2 cut(s) 2144, 2176
BpmI CTGGAG 1 cut(s) 327
Bpu10I CCTNAGC 2 cut(s) 1633, 2370
BpuEI CTTGAG 3 cut(s) 133, 1481, 2281
BpuMI CCSGG 2 cut(s) 960, 1481
BsaI GGTCTC 1 cut(s) 356
BsaJI CCNNGG 9 cut(s) 102, 196, 529, 604, 713, 959, 1380, 1984, 2304
BsaWI WCCGGW 1 cut(s) 876
BsaXI ACNNNNNCTCC 2 cut(s) 845, 875
Bsc4I CCNNNNNNNGG 4 cut(s) 324, 611, 693, 1999
Bse118I RCCGGY 1 cut(s) 876
Bse1I ACTGG 4 cut(s) 366, 565, 616, 890
BseBI CCWGG 5 cut(s) 103, 198, 531, 606, 1382
BseDI CCNNGG 9 cut(s) 102, 196, 529, 604, 713, 959, 1380, 1984, 2304
BseGI GGATG 8 cut(s) 142, 291, 552, 1019, 1459, 1746, 2089, 2324
BseLI CCNNNNNNNGG 4 cut(s) 324, 611, 693, 1999
BseMII CTCAG 3 cut(s) 1296, 1647, 1971
BseNI ACTGG 4 cut(s) 366, 565, 616, 890
BseRI GAGGAG 3 cut(s) 71, 1746, 2165
BseXI GCAGC 6 cut(s) 183, 1118, 1529, 1641, 2386, 2398
BshFI GGCC 4 cut(s) 11, 718, 1262, 1379
BshNI GGYRCC 1 cut(s) 902
BshTI ACCGGT 1 cut(s) 876
BsiHKAI GWGCWC 1 cut(s) 2470
BsiHKCI CYCGRG 1 cut(s) 1882
BsiSI CCGG 3 cut(s) 877, 959, 1481
BslFI GGGAC 3 cut(s) 119, 629, 2003
BslI CCNNNNNNNGG 4 cut(s) 324, 611, 693, 1999
BsmAI GTCTC 4 cut(s) 276, 356, 396, 471
BsmFI GGGAC 3 cut(s) 119, 629, 2003
BsmI GAATGC 2 cut(s) 1315, 2268
BsnI GGCC 4 cut(s) 11, 718, 1262, 1379
Bso31I GGTCTC 1 cut(s) 356
BsoBI CYCGRG 1 cut(s) 1882
Bsp1286I GDGCHC 1 cut(s) 2470
Bsp1407I TGTACA 1 cut(s) 430
Bsp143I GATC 7 cut(s) 116, 270, 841, 1087, 1270, 1498, 1906
Bsp19I CCATGG 1 cut(s) 1984
BspACI CCGC 2 cut(s) 214, 1314
BspANI GGCC 4 cut(s) 11, 718, 1262, 1379
BspCNI CTCAG 3 cut(s) 1297, 1646, 1970
BspLI GGNNCC 3 cut(s) 644, 904, 1991
BspMAI CTGCAG 1 cut(s) 385
BspPI GGATC 3 cut(s) 278, 1082, 1914
BspT107I GGYRCC 1 cut(s) 902
BspTI CTTAAG 1 cut(s) 1415
BspTNI GGTCTC 1 cut(s) 356
BsrFI RCCGGY 1 cut(s) 876
BsrGI TGTACA 1 cut(s) 430
BsrI ACTGG 4 cut(s) 366, 565, 616, 890
BssAI RCCGGY 1 cut(s) 876
BssECI CCNNGG 9 cut(s) 102, 196, 529, 604, 713, 959, 1380, 1984, 2304
BssMI GATC 7 cut(s) 116, 270, 841, 1087, 1270, 1498, 1906
BssSI CACGAG 1 cut(s) 1854
BssT1I CCWWGG 3 cut(s) 713, 1984, 2304
Bst2BI CACGAG 1 cut(s) 1854
Bst2UI CCWGG 5 cut(s) 103, 198, 531, 606, 1382
Bst4CI ACNGT 6 cut(s) 262, 652, 663, 929, 1365, 2283
Bst6I CTCTTC 3 cut(s) 80, 89, 2364
BstAFI CTTAAG 1 cut(s) 1415
BstAUI TGTACA 1 cut(s) 430
BstC8I GCNNGC 7 cut(s) 1145, 1346, 1377, 1401, 2129, 2291, 2379
BstDSI CCRYGG 1 cut(s) 1984
BstF5I GGATG 8 cut(s) 142, 291, 552, 1019, 1459, 1746, 2089, 2324
BstKTI GATC 7 cut(s) 119, 273, 844, 1090, 1273, 1501, 1909
BstMAI GTCTC 4 cut(s) 276, 356, 396, 471
BstMBI GATC 7 cut(s) 116, 270, 841, 1087, 1270, 1498, 1906
BstMWI GCNNNNNNNGC 5 cut(s) 814, 918, 1103, 1310, 1376
BstNI CCWGG 5 cut(s) 103, 198, 531, 606, 1382
BstNSI RCATGY 4 cut(s) 1348, 2041, 2183, 2192
BstPAI GACNNNNGTC 1 cut(s) 1436
BstSCI CCNGG 7 cut(s) 101, 196, 529, 604, 958, 1380, 1479
BstSFI CTRYAG 2 cut(s) 381, 513
BstV1I GCAGC 6 cut(s) 183, 1118, 1529, 1641, 2386, 2398
BstV2I GAAGAC 2 cut(s) 1436, 2380
BstX2I RGATCY 3 cut(s) 1087, 1270, 1906
BstYI RGATCY 3 cut(s) 1087, 1270, 1906
BsuI GTATCC 1 cut(s) 436
BsuRI GGCC 4 cut(s) 11, 718, 1262, 1379
BtgI CCRYGG 1 cut(s) 1984
BtgZI GCGATG 1 cut(s) 2214
BtsCI GGATG 8 cut(s) 142, 291, 552, 1019, 1459, 1746, 2089, 2324
BtsI GCAGTG 2 cut(s) 2061, 2413
BtsIMutI CAGTG 5 cut(s) 267, 648, 934, 2061, 2413
Cac8I GCNNGC 7 cut(s) 1145, 1346, 1377, 1401, 2129, 2291, 2379
Cfr10I RCCGGY 1 cut(s) 876
Cfr13I GGNCC 6 cut(s) 10, 727, 1378, 1806, 1990, 2308
CpoI CGGWCCG 1 cut(s) 727
CseI GACGC 1 cut(s) 1103
Csp6I GTAC 4 cut(s) 431, 1131, 1385, 2105
CspAI ACCGGT 1 cut(s) 876
CspI CGGWCCG 1 cut(s) 727
CviQI GTAC 4 cut(s) 431, 1131, 1385, 2105
DpnI GATC 7 cut(s) 118, 272, 843, 1089, 1272, 1500, 1908
DpnII GATC 7 cut(s) 116, 270, 841, 1087, 1270, 1498, 1906
Eam1104I CTCTTC 3 cut(s) 80, 89, 2364
EarI CTCTTC 3 cut(s) 80, 89, 2364
Ecl136II GAGCTC 1 cut(s) 2468
Eco130I CCWWGG 3 cut(s) 713, 1984, 2304
Eco24I GRGCYC 1 cut(s) 2470
Eco31I GGTCTC 1 cut(s) 356
Eco47I GGWCC 4 cut(s) 727, 1806, 1990, 2308
Eco53kI GAGCTC 1 cut(s) 2468
Eco57I CTGAAG 5 cut(s) 152, 175, 1318, 1982, 2374
Eco88I CYCGRG 1 cut(s) 1882
EcoICRI GAGCTC 1 cut(s) 2468
EcoO109I RGGNCCY 2 cut(s) 10, 1806
EcoRI GAATTC 1 cut(s) 1652
EcoRII CCWGG 5 cut(s) 101, 196, 529, 604, 1380
EcoT14I CCWWGG 3 cut(s) 713, 1984, 2304
EcoT22I ATGCAT 1 cut(s) 2270
EcoT38I GRGCYC 1 cut(s) 2470
ErhI CCWWGG 3 cut(s) 713, 1984, 2304
FalI AAGNNNNNCTT 3 cut(s) 31, 2415, 2447
FaqI GGGAC 3 cut(s) 119, 629, 2003
FauNDI CATATG 2 cut(s) 427, 2029
FblI GTMKAC 2 cut(s) 863, 955
Fnu4HI GCNGC 7 cut(s) 172, 1107, 1314, 1518, 1630, 2375, 2412
FokI GGATG 8 cut(s) 149, 278, 559, 1026, 1466, 1753, 2096, 2311
FriOI GRGCYC 1 cut(s) 2470
Fsp4HI GCNGC 7 cut(s) 172, 1107, 1314, 1518, 1630, 2375, 2412
FspBI CTAG 6 cut(s) 207, 1511, 1626, 1745, 1751, 1931
GluI GCNGC 7 cut(s) 172, 1107, 1314, 1518, 1630, 2375, 2412
GsuI CTGGAG 1 cut(s) 327
HaeIII GGCC 4 cut(s) 11, 718, 1262, 1379
HapII CCGG 3 cut(s) 877, 959, 1481
HgaI GACGC 1 cut(s) 1103
HindIII AAGCTT 2 cut(s) 1706, 1771
HpaII CCGG 3 cut(s) 877, 959, 1481
HphI GGTGA 9 cut(s) 307, 713, 848, 1093, 1595, 1661, 1865, 2034, 2360
Hpy166II GTNNAC 5 cut(s) 149, 864, 956, 1025, 1252
Hpy188I TCNGA 8 cut(s) 253, 731, 1009, 1503, 1537, 1960, 2083, 2393
Hpy8I GTNNAC 5 cut(s) 149, 864, 956, 1025, 1252
HpyCH4III ACNGT 6 cut(s) 262, 652, 663, 929, 1365, 2283
HpyF10VI GCNNNNNNNGC 5 cut(s) 814, 918, 1103, 1310, 1376
Kzo9I GATC 7 cut(s) 116, 270, 841, 1087, 1270, 1498, 1906
LmnI GCTCC 2 cut(s) 642, 2465
Lsp1109I GCAGC 6 cut(s) 183, 1118, 1529, 1641, 2386, 2398
LweI GCATC 4 cut(s) 1106, 1731, 2255, 2363
MaeI CTAG 6 cut(s) 207, 1511, 1626, 1745, 1751, 1931
MaeIII GTNAC 6 cut(s) 591, 652, 836, 881, 1587, 2040
MalI GATC 7 cut(s) 118, 272, 843, 1089, 1272, 1500, 1908
MboI GATC 7 cut(s) 116, 270, 841, 1087, 1270, 1498, 1906
MflI RGATCY 3 cut(s) 1087, 1270, 1906
MhlI GDGCHC 1 cut(s) 2470
MlyI GAGTC 3 cut(s) 1038, 1772, 1874
MmeI TCCRAC 2 cut(s) 968, 2002
Mph1103I ATGCAT 1 cut(s) 2270
MroXI GAANNNNTTC 2 cut(s) 1165, 2398
MseI TTAA 7 cut(s) 524, 578, 1416, 1542, 1889, 2439, 2447
MslI CAYNNNNRTG 1 cut(s) 900
MspCI CTTAAG 1 cut(s) 1415
MspI CCGG 3 cut(s) 877, 959, 1481
MspR9I CCNGG 7 cut(s) 103, 198, 531, 606, 960, 1382, 1481
Mva1269I GAATGC 2 cut(s) 1315, 2268
MvaI CCWGG 5 cut(s) 103, 198, 531, 606, 1382
MwoI GCNNNNNNNGC 5 cut(s) 814, 918, 1103, 1310, 1376
NciI CCSGG 2 cut(s) 960, 1481
NcoI CCATGG 1 cut(s) 1984
NdeI CATATG 2 cut(s) 427, 2029
NdeII GATC 7 cut(s) 116, 270, 841, 1087, 1270, 1498, 1906
NlaIV GGNNCC 3 cut(s) 644, 904, 1991
NmuCI GTSAC 5 cut(s) 591, 652, 836, 881, 2040
NsiI ATGCAT 1 cut(s) 2270
NspI RCATGY 4 cut(s) 1348, 2041, 2183, 2192
PaeI GCATGC 1 cut(s) 1348
PaeR7I CTCGAG 1 cut(s) 1882
PciI ACATGT 2 cut(s) 2037, 2179
PctI GAATGC 2 cut(s) 1315, 2268
PdmI GAANNNNTTC 2 cut(s) 1165, 2398
PfeI GAWTC 8 cut(s) 483, 1329, 1696, 1837, 2067, 2225, 2300, 2417
PflMI CCANNNNNTGG 1 cut(s) 693
PinAI ACCGGT 1 cut(s) 876
PkrI GCNGC 7 cut(s) 173, 1108, 1315, 1519, 1631, 2376, 2413
PleI GAGTC 3 cut(s) 1037, 1772, 1874
PpsI GAGTC 3 cut(s) 1037, 1772, 1874
PpuMI RGGWCCY 1 cut(s) 1806
PscI ACATGT 2 cut(s) 2037, 2179
PshAI GACNNNNGTC 1 cut(s) 1436
PsiI TTATAA 4 cut(s) 354, 675, 765, 1578
Psp124BI GAGCTC 1 cut(s) 2470
Psp5II RGGWCCY 1 cut(s) 1806
Psp6I CCWGG 5 cut(s) 101, 196, 529, 604, 1380
PspGI CCWGG 5 cut(s) 101, 196, 529, 604, 1380
PspN4I GGNNCC 3 cut(s) 644, 904, 1991
PspPI GGNCC 6 cut(s) 10, 727, 1378, 1806, 1990, 2308
PspPPI RGGWCCY 1 cut(s) 1806
PstI CTGCAG 1 cut(s) 385
PsuI RGATCY 3 cut(s) 1087, 1270, 1906
RsaI GTAC 4 cut(s) 432, 1132, 1386, 2106
RsaNI GTAC 4 cut(s) 431, 1131, 1385, 2105
RseI CAYNNNNRTG 1 cut(s) 900
Rsr2I CGGWCCG 1 cut(s) 727
RsrII CGGWCCG 1 cut(s) 727
SacI GAGCTC 1 cut(s) 2470
SaqAI TTAA 7 cut(s) 524, 578, 1416, 1542, 1889, 2439, 2447
SatI GCNGC 7 cut(s) 172, 1107, 1314, 1518, 1630, 2375, 2412
Sau3AI GATC 7 cut(s) 116, 270, 841, 1087, 1270, 1498, 1906
Sau96I GGNCC 6 cut(s) 10, 727, 1378, 1806, 1990, 2308
SchI GAGTC 3 cut(s) 1038, 1772, 1874
ScrFI CCNGG 7 cut(s) 103, 198, 531, 606, 960, 1382, 1481
SduI GDGCHC 1 cut(s) 2470
SfaNI GCATC 4 cut(s) 1106, 1731, 2255, 2363
SfcI CTRYAG 2 cut(s) 381, 513
Sfr274I CTCGAG 1 cut(s) 1882
SinI GGWCC 4 cut(s) 727, 1806, 1990, 2308
SlaI CTCGAG 1 cut(s) 1882
SmiMI CAYNNNNRTG 1 cut(s) 900
SmlI CTYRAG 5 cut(s) 112, 1415, 1460, 1882, 2260
SmoI CTYRAG 5 cut(s) 112, 1415, 1460, 1882, 2260
SpeI ACTAGT 1 cut(s) 1510
SphI GCATGC 1 cut(s) 1348
SsiI CCGC 2 cut(s) 214, 1314
SspI AATATT 1 cut(s) 1924
SspMI CTAG 6 cut(s) 207, 1511, 1626, 1745, 1751, 1931
SstI GAGCTC 1 cut(s) 2470
StyD4I CCNGG 7 cut(s) 101, 196, 529, 604, 958, 1380, 1479
StyI CCWWGG 3 cut(s) 713, 1984, 2304
TaaI ACNGT 6 cut(s) 262, 652, 663, 929, 1365, 2283
TaqI TCGA 7 cut(s) 119, 504, 942, 1100, 1497, 1883, 2470
TaqII GACCGA 2 cut(s) 715, 2325
TatI WGTACW 2 cut(s) 430, 2104
TauI GCSGC 1 cut(s) 1316
TfiI GAWTC 8 cut(s) 483, 1329, 1696, 1837, 2067, 2225, 2300, 2417
Tru1I TTAA 7 cut(s) 524, 578, 1416, 1542, 1889, 2439, 2447
Tru9I TTAA 7 cut(s) 524, 578, 1416, 1542, 1889, 2439, 2447
TscAI CASTG 5 cut(s) 267, 655, 934, 2061, 2413
TseFI GTSAC 5 cut(s) 591, 652, 836, 881, 2040
TseI GCWGC 6 cut(s) 171, 1106, 1517, 1629, 2374, 2411
Tsp45I GTSAC 5 cut(s) 591, 652, 836, 881, 2040
TspGWI ACGGA 1 cut(s) 2317
TspRI CASTG 5 cut(s) 267, 655, 934, 2061, 2413
Van91I CCANNNNNTGG 1 cut(s) 693
Vha464I CTTAAG 1 cut(s) 1415
VpaK11BI GGWCC 4 cut(s) 727, 1806, 1990, 2308
XapI RAATTY 4 cut(s) 202, 1652, 1977, 2398
XbaI TCTAGA 1 cut(s) 1930
XceI RCATGY 4 cut(s) 1348, 2041, 2183, 2192
XcmI CCANNNNNNNNNTGG 1 cut(s) 302
XhoI CTCGAG 1 cut(s) 1882
XmiI GTMKAC 2 cut(s) 863, 955
XmnI GAANNNNTTC 2 cut(s) 1165, 2398
XspI CTAG 6 cut(s) 207, 1511, 1626, 1745, 1751, 1931
Zsp2I ATGCAT 1 cut(s) 2270
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.