Rw3G018850

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000112
Physical Location & Seq
Forward (+)
21125414 .. 21128699
3286 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G018850.1

Sequence Viewer

Length: 2496 bp
ATGAAAAAGGACCTCTGGTCATTTTTTATATTCACACATAGCCAGATTGTAAATGCCCTGAAAATGCTGTTGAAATCTCTGCTTCTCTTCCTCCTCTTCCAAGTTTGCACTTCCAGGGACACCTTGAGATCGAACGAAGAACTCAAGGATGATGATGGTGGACTTCTGGTGTCCAAAGAAAGCAGCTTTGAGTTAGGTTTCTTCAGTCCTGGAAATTCTAGCAACCGCTATGTTGGGATTTGGTATTCTCAATCTAAAGTATCTGAGAAAACAGTGGTGTGGATCGCAAACAGAGACAACCCCATCAATGATACCTCTGGAGTTTTCACCATAAACAGGTATGGAGACCTAGTTCTTTATGCTTATAACATGGAGACCAGTCCTATATGGTCTGCAGATGCGTCTCTTTCAGTCAAAAACACCAGCACCTTATCTGCATATGTACAGCTTTTGGATACAGGAAACTTAGTTATGTTCCAGAATGATAGAGACGGAATCTTTGTATGGCAAAGTTTTGATTATCCTACAGATACTTTAATCCCTGGTATGAAAGTTGGGATGAATCGGAAAACTAGGCTAGAATGGGTTTTAACATCTTGGAAGTCACTAGATGACCCTGGAACTGGGGACTATACCTTTAGGCTTCATAATAGGGGCCACATTTTCACACCCCAGTCTTTTATTTATAAAGGTTTGTCTAAGTATTGGCGAGGTGATACAGAGCCTTGGCCTAATTCGATCCGAAATGCAGAGGAAATTTCTTTTTTCCTTTCTTATAACACCAATAAACTTACAATAATGAAGTTGATGTTTTCTGGGCTACATCAGCACTTGGTATGGAGTGATGCTGACCATCAATGGAAGGAGTTGTGGTCTTCACCGAAATACAGGTGTGACTGGTATGGACATTGTGGTGCCAACAGCAAATGTAGCCTTGACAGTGGTAATCTGTTTGAGTGTGAGTGTCTACCGGGGTATGAACCAAAATCTATAAATGGTTGGAATGGGAGAGATGGGTCAGATGGATGTGTGAGTAAACGAGTCAAAGTGTCAAAGTGTAGAAATGGAGAAGGGTTTGTGAAGGTGGAAAGGGTGAAAGATCCAGACGCATCGAGAGCAGCATTGTTGGAAAGAGGTATGAGTACCAAAGAGTGTGAGCAGGAGTGCCTAAGAAATTGTTCTTGCACGGCATATGTGACGAAAAAGATTGAAGGGGTTGTTAATTGCTTGACATGGTATGATGACTTGATGGACATTTTGGTTTACACAGAGGCCGGACGAGATCTGTATGTTCGTGTGGATAAAATCGAGTTAGCTGAGAATGCCGCAAACTCACAAGGATTCTTCAGGAGGGGCATGCTTGTTATTCCAGTACTGTTTGCTCTGCTGGCCCTAGTACTAATAAATGTGCTTGCCTGTTGGTTGCTTAAGAAAAAAGGGAAGACAAAAGTCTTTGTGGATGTGGATGAACTTGAGGAAAGCAAGAGACACCCGGAATTGCAATTTTTCGATCTGAACACACTAGTAGCAGCCACAGATAATTTCTCTGATGTTAATAAACTTGGCGAAGGTGGTTTTGGTGGTGTTTATAAGGGTGAGTTACCAAATGGACAGAAGGTTGCTGTGAAAAGACTGTCTAGCAGCTCAGGACAAGGGATTGGTGAATTCAAAAATGAAGTTGAACTGATAGCAGGACTTCAACACAGGAATCTTGTGAAGCTTTTAGGTTGTTGTATAAAGGGAGGAGAAAGGATGCTAGTCCTCGAATATATGCCGAACAAAAGCTTGGACTCATTTCTTTTTGATGACACTAGAAGGTCCTTCTTAGATTGGGGAAGGCGTTTTGAGATTATCAATGGGGTTGCTCGTGGGCTTTTGTATCTTCACCAAGACTCGAGATTAAGGATTATTCATAGGGATCTAAAAACCAGCAATATTCTTCTAGATGATGAGATGACCCCAAAAATCTCAGATTTCGGCATGGCAAGAATATTCCATGGGGACCAACTTCAGGATAACACAAAGAAAGTTGTTGGAACATATGGCTATATGTCACCAGAGTATGCAGTGTTTGGGAGATTCTCTATGAAATCGGATGTGTTTAGTTTTGGGATTATACTATTGGAGATTGTTAGTGGCAAGAGAAACAATGGTTCTTATGAGGAGAATTACTCCATGAACCTGATAGGACATGTTTGGCATGTGTGGAAAGAAGGAATAGCCTTAGATATTGTGGATTCATCGCTACAATCATATAAGCATGATGAAGTTTTGAGATGCATTCAAGTTGGACTGTTGTGCGTGCAAGAAGATTCAAAGGACCGGCCAGCCATGTCATCCATTGTGTTCATGTTGAGTGGTGAGGCATCTCTTCCATCTCCTAAGCAGCCTGCATTTGTCTTCCGAAGAAATTCCAGCAATGCTGCTGATTGCTCAAGTCCAAAAGAATTGCATTCTATTAACGACTTGACAATAACTATTGTGGGAGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

831

Amino Acids

94.1

Weight (kDa)

6.62

Isoelectric Point (pI)

39.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 91 - 201 1.7e-34 D-mannose binding lectin
S_locus_glycop PF00954 250 - 330 7.3e-15 S-locus glycoprotein domain
PAN_2 PF08276 353 - 419 1.5e-17 PAN-like domain
Pkinase PF00069 516 - 780 2e-43 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 519 - 782 1.9e-47 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 788 - 831 6.3e-09 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 366, 687, 777, 1590
AccB1I GGYRCC 1 cut(s) 914
AccI GTMKAC 1 cut(s) 967
AciI CCGC 2 cut(s) 226, 1326
AclWI GGATC 4 cut(s) 290, 733, 1094, 1926
AcoI YGGCCR 1 cut(s) 2324
AcsI RAATTY 4 cut(s) 214, 756, 1664, 2410
AcuI CTGAAG 3 cut(s) 187, 1330, 1994
AfaI GTAC 4 cut(s) 444, 1144, 1374, 1398
AfiI CCNNNNNNNGG 3 cut(s) 336, 623, 2011
AflII CTTAAG 1 cut(s) 1427
AflIII ACRYGT 1 cut(s) 2191
AgsI TTSAA 7 cut(s) 73, 1211, 1669, 1682, 1700, 2285, 2316
AhdI GACNNNNNGTC 1 cut(s) 16
AhlI ACTAGT 1 cut(s) 1522
AjnI CCWGG 4 cut(s) 113, 208, 541, 616
AjuI GAANNNNNNNTTGG 4 cut(s) 1560, 1592, 1769, 1801
AloI GAACNNNNNNTCC 2 cut(s) 336, 368
AluBI AGCT 7 cut(s) 186, 448, 1316, 1644, 1720, 1785, 2489
AluI AGCT 7 cut(s) 186, 448, 1316, 1644, 1720, 1785, 2489
Alw21I GWGCWC 1 cut(s) 2491
Alw26I GTCTC 6 cut(s) 288, 339, 368, 408, 483, 1480
AlwI GGATC 4 cut(s) 290, 733, 1094, 1926
Ama87I CYCGRG 1 cut(s) 1894
AoxI GGCC 5 cut(s) 655, 728, 1272, 1389, 2324
ApeKI GCWGC 6 cut(s) 183, 1118, 1529, 1641, 2386, 2423
ApoI RAATTY 4 cut(s) 214, 756, 1664, 2410
ArsI GACNNNNNNTTYG 2 cut(s) 482, 514
Asp700I GAANNNNTTC 2 cut(s) 1177, 2410
AspS9I GGNCC 6 cut(s) 10, 655, 1390, 1818, 2002, 2320
AsuC2I CCSGG 2 cut(s) 972, 1493
AsuHPI GGTGA 9 cut(s) 319, 725, 870, 1105, 1607, 1673, 1877, 2046, 2372
AvaI CYCGRG 1 cut(s) 1894
AvaII GGWCC 4 cut(s) 10, 1818, 2002, 2320
BanI GGYRCC 1 cut(s) 914
BanII GRGCYC 1 cut(s) 2491
BauI CACGAG 1 cut(s) 1866
BbsI GAAGAC 3 cut(s) 867, 1448, 2392
Bbv12I GWGCWC 1 cut(s) 2491
BbvI GCAGC 6 cut(s) 195, 1130, 1541, 1653, 2398, 2410
BccI CCATC 7 cut(s) 149, 311, 861, 1007, 1016, 1243, 2383
BceAI ACGGC 1 cut(s) 1203
BciT130I CCWGG 4 cut(s) 115, 210, 543, 618
BciVI GTATCC 1 cut(s) 448
BcnI CCSGG 2 cut(s) 972, 1493
BcoDI GTCTC 6 cut(s) 288, 339, 368, 408, 483, 1480
BcuI ACTAGT 1 cut(s) 1522
BfmI CTRYAG 2 cut(s) 393, 525
BfrI CTTAAG 1 cut(s) 1427
BfuI GTATCC 1 cut(s) 448
BglII AGATCT 1 cut(s) 1282
BisI GCNGC 7 cut(s) 184, 1119, 1326, 1530, 1642, 2387, 2424
BlsI GCNGC 7 cut(s) 185, 1120, 1327, 1531, 1643, 2388, 2425
BmcAI AGTACT 2 cut(s) 1374, 1398
Bme1390I CCNGG 6 cut(s) 115, 210, 543, 618, 972, 1493
Bme18I GGWCC 4 cut(s) 10, 1818, 2002, 2320
BmeRI GACNNNNNGTC 1 cut(s) 16
BmeT110I CYCGRG 1 cut(s) 1894
BmgT120I GGNCC 6 cut(s) 10, 655, 1390, 1818, 2002, 2320
BmiI GGNNCC 3 cut(s) 656, 916, 2003
BmrFI CCNGG 6 cut(s) 115, 210, 543, 618, 972, 1493
BmrI ACTGGG 2 cut(s) 633, 667
BmsI GCATC 6 cut(s) 388, 835, 1118, 1743, 2267, 2375
BmuI ACTGGG 2 cut(s) 633, 667
BoxI GACNNNNGTC 1 cut(s) 1448
BpiI GAAGAC 3 cut(s) 867, 1448, 2392
BplI GAGNNNNNCTC 2 cut(s) 2156, 2188
BpmI CTGGAG 1 cut(s) 339
Bpu10I CCTNAGC 2 cut(s) 1645, 2382
BpuEI CTTGAG 4 cut(s) 128, 145, 1493, 2419
BpuMI CCSGG 2 cut(s) 972, 1493
BsaI GGTCTC 2 cut(s) 339, 368
BsaJI CCNNGG 6 cut(s) 114, 541, 616, 725, 971, 1996
BsaXI ACNNNNNCTCC 4 cut(s) 336, 366, 857, 887
Bsc4I CCNNNNNNNGG 3 cut(s) 336, 623, 2011
Bse118I RCCGGY 1 cut(s) 2322
Bse1I ACTGG 5 cut(s) 378, 628, 673, 902, 1370
Bse3DI GCAATG 1 cut(s) 2425
BseBI CCWGG 4 cut(s) 115, 210, 543, 618
BseDI CCNNGG 6 cut(s) 114, 541, 616, 725, 971, 1996
BseGI GGATG 8 cut(s) 154, 564, 1031, 1465, 1471, 1758, 2101, 2336
BseLI CCNNNNNNNGG 3 cut(s) 336, 623, 2011
BseMI GCAATG 1 cut(s) 2425
BseMII CTCAG 4 cut(s) 255, 1308, 1659, 1983
BseNI ACTGG 5 cut(s) 378, 628, 673, 902, 1370
BseRI GAGGAG 3 cut(s) 83, 1758, 2177
BseXI GCAGC 6 cut(s) 195, 1130, 1541, 1653, 2398, 2410
BshFI GGCC 5 cut(s) 657, 730, 1274, 1391, 2326
BshNI GGYRCC 1 cut(s) 914
BsiHKAI GWGCWC 1 cut(s) 2491
BsiHKCI CYCGRG 1 cut(s) 1894
BsiSI CCGG 4 cut(s) 971, 1275, 1493, 2323
BslFI GGGAC 3 cut(s) 131, 641, 2015
BslI CCNNNNNNNGG 3 cut(s) 336, 623, 2011
BsmAI GTCTC 6 cut(s) 288, 339, 368, 408, 483, 1480
BsmBI CGTCTC 2 cut(s) 408, 483
BsmFI GGGAC 3 cut(s) 131, 641, 2015
BsmI GAATGC 3 cut(s) 1327, 2280, 2452
BsnI GGCC 5 cut(s) 657, 730, 1274, 1391, 2326
Bso31I GGTCTC 2 cut(s) 339, 368
BsoBI CYCGRG 1 cut(s) 1894
Bsp1286I GDGCHC 1 cut(s) 2491
Bsp1407I TGTACA 1 cut(s) 442
Bsp143I GATC 7 cut(s) 128, 282, 738, 1099, 1282, 1510, 1918
Bsp19I CCATGG 1 cut(s) 1996
BspACI CCGC 2 cut(s) 226, 1326
BspANI GGCC 5 cut(s) 657, 730, 1274, 1391, 2326
BspCNI CTCAG 4 cut(s) 256, 1309, 1658, 1982
BspLI GGNNCC 3 cut(s) 656, 916, 2003
BspMAI CTGCAG 1 cut(s) 397
BspPI GGATC 4 cut(s) 290, 733, 1094, 1926
BspT107I GGYRCC 1 cut(s) 914
BspTI CTTAAG 1 cut(s) 1427
BspTNI GGTCTC 2 cut(s) 339, 368
BsrDI GCAATG 1 cut(s) 2425
BsrFI RCCGGY 1 cut(s) 2322
BsrGI TGTACA 1 cut(s) 442
BsrI ACTGG 5 cut(s) 378, 628, 673, 902, 1370
BssAI RCCGGY 1 cut(s) 2322
BssECI CCNNGG 6 cut(s) 114, 541, 616, 725, 971, 1996
BssMI GATC 7 cut(s) 128, 282, 738, 1099, 1282, 1510, 1918
BssSI CACGAG 1 cut(s) 1866
BssT1I CCWWGG 2 cut(s) 725, 1996
Bst2BI CACGAG 1 cut(s) 1866
Bst2UI CCWGG 4 cut(s) 115, 210, 543, 618
Bst4CI ACNGT 5 cut(s) 274, 941, 1377, 1635, 2295
Bst6I CTCTTC 3 cut(s) 92, 101, 2376
BstAFI CTTAAG 1 cut(s) 1427
BstAUI TGTACA 1 cut(s) 442
BstC8I GCNNGC 6 cut(s) 1358, 1389, 1413, 2303, 2328, 2391
BstDSI CCRYGG 1 cut(s) 1996
BstF5I GGATG 8 cut(s) 154, 564, 1031, 1465, 1471, 1758, 2101, 2336
BstKTI GATC 7 cut(s) 131, 285, 741, 1102, 1285, 1513, 1921
BstMAI GTCTC 6 cut(s) 288, 339, 368, 408, 483, 1480
BstMBI GATC 7 cut(s) 128, 282, 738, 1099, 1282, 1510, 1918
BstMWI GCNNNNNNNGC 5 cut(s) 826, 930, 1115, 1322, 1388
BstNI CCWGG 4 cut(s) 115, 210, 543, 618
BstNSI RCATGY 3 cut(s) 1360, 2195, 2204
BstPAI GACNNNNGTC 1 cut(s) 1448
BstSCI CCNGG 6 cut(s) 113, 208, 541, 616, 970, 1491
BstSFI CTRYAG 2 cut(s) 393, 525
BstV1I GCAGC 6 cut(s) 195, 1130, 1541, 1653, 2398, 2410
BstV2I GAAGAC 3 cut(s) 867, 1448, 2392
BstX2I RGATCY 3 cut(s) 1099, 1282, 1918
BstYI RGATCY 3 cut(s) 1099, 1282, 1918
BsuI GTATCC 1 cut(s) 448
BsuRI GGCC 5 cut(s) 657, 730, 1274, 1391, 2326
BtgI CCRYGG 1 cut(s) 1996
BtgZI GCGATG 1 cut(s) 2226
BtsCI GGATG 8 cut(s) 154, 564, 1031, 1465, 1471, 1758, 2101, 2336
BtsI GCAGTG 1 cut(s) 2073
BtsIMutI CAGTG 3 cut(s) 279, 946, 2073
Cac8I GCNNGC 6 cut(s) 1358, 1389, 1413, 2303, 2328, 2391
Cfr10I RCCGGY 1 cut(s) 2322
Cfr13I GGNCC 6 cut(s) 10, 655, 1390, 1818, 2002, 2320
CseI GACGC 2 cut(s) 390, 1115
Csp6I GTAC 4 cut(s) 443, 1143, 1373, 1397
CviQI GTAC 4 cut(s) 443, 1143, 1373, 1397
DpnI GATC 7 cut(s) 130, 284, 740, 1101, 1284, 1512, 1920
DpnII GATC 7 cut(s) 128, 282, 738, 1099, 1282, 1510, 1918
DriI GACNNNNNGTC 1 cut(s) 16
EaeI YGGCCR 1 cut(s) 2324
Eam1104I CTCTTC 3 cut(s) 92, 101, 2376
Eam1105I GACNNNNNGTC 1 cut(s) 16
EarI CTCTTC 3 cut(s) 92, 101, 2376
Ecl136II GAGCTC 1 cut(s) 2489
Eco130I CCWWGG 2 cut(s) 725, 1996
Eco24I GRGCYC 1 cut(s) 2491
Eco31I GGTCTC 2 cut(s) 339, 368
Eco47I GGWCC 4 cut(s) 10, 1818, 2002, 2320
Eco53kI GAGCTC 1 cut(s) 2489
Eco57I CTGAAG 3 cut(s) 187, 1330, 1994
Eco88I CYCGRG 1 cut(s) 1894
EcoICRI GAGCTC 1 cut(s) 2489
EcoO109I RGGNCCY 2 cut(s) 10, 1818
EcoRI GAATTC 1 cut(s) 1664
EcoRII CCWGG 4 cut(s) 113, 208, 541, 616
EcoT14I CCWWGG 2 cut(s) 725, 1996
EcoT22I ATGCAT 1 cut(s) 2282
EcoT38I GRGCYC 1 cut(s) 2491
ErhI CCWWGG 2 cut(s) 725, 1996
Esp3I CGTCTC 2 cut(s) 408, 483
FaqI GGGAC 3 cut(s) 131, 641, 2015
FauNDI CATATG 3 cut(s) 439, 1192, 2041
FblI GTMKAC 1 cut(s) 967
Fnu4HI GCNGC 7 cut(s) 184, 1119, 1326, 1530, 1642, 2387, 2424
FokI GGATG 8 cut(s) 161, 571, 1038, 1472, 1478, 1765, 2108, 2323
FriOI GRGCYC 1 cut(s) 2491
Fsp4HI GCNGC 7 cut(s) 184, 1119, 1326, 1530, 1642, 2387, 2424
GluI GCNGC 7 cut(s) 184, 1119, 1326, 1530, 1642, 2387, 2424
GsuI CTGGAG 1 cut(s) 339
HaeIII GGCC 5 cut(s) 657, 730, 1274, 1391, 2326
HapII CCGG 4 cut(s) 971, 1275, 1493, 2323
HgaI GACGC 2 cut(s) 390, 1115
HindIII AAGCTT 2 cut(s) 1718, 1783
HpaII CCGG 4 cut(s) 971, 1275, 1493, 2323
HphI GGTGA 9 cut(s) 319, 725, 870, 1105, 1607, 1673, 1877, 2046, 2372
Hpy166II GTNNAC 4 cut(s) 161, 968, 1037, 1264
Hpy188I TCNGA 9 cut(s) 265, 567, 743, 1021, 1515, 1549, 1972, 2095, 2405
Hpy188III TCNNGA 9 cut(s) 318, 478, 1103, 1113, 1348, 1647, 1896, 1943, 2012
Hpy8I GTNNAC 4 cut(s) 161, 968, 1037, 1264
HpyCH4III ACNGT 5 cut(s) 274, 941, 1377, 1635, 2295
HpyF10VI GCNNNNNNNGC 5 cut(s) 826, 930, 1115, 1322, 1388
Kzo9I GATC 7 cut(s) 128, 282, 738, 1099, 1282, 1510, 1918
LmnI GCTCC 1 cut(s) 2486
Lsp1109I GCAGC 6 cut(s) 195, 1130, 1541, 1653, 2398, 2410
LweI GCATC 6 cut(s) 388, 835, 1118, 1743, 2267, 2375
MaeIII GTNAC 5 cut(s) 603, 893, 1195, 1599, 2052
MalI GATC 7 cut(s) 130, 284, 740, 1101, 1284, 1512, 1920
MboI GATC 7 cut(s) 128, 282, 738, 1099, 1282, 1510, 1918
MflI RGATCY 3 cut(s) 1099, 1282, 1918
MhlI GDGCHC 1 cut(s) 2491
MlyI GAGTC 3 cut(s) 1050, 1784, 1886
MmeI TCCRAC 4 cut(s) 980, 1107, 2014, 2269
Mph1103I ATGCAT 1 cut(s) 2282
MroXI GAANNNNTTC 2 cut(s) 1177, 2410
MseI TTAA 7 cut(s) 536, 590, 1221, 1428, 1554, 1901, 2460
MslI CAYNNNNRTG 1 cut(s) 912
MspCI CTTAAG 1 cut(s) 1427
MspI CCGG 4 cut(s) 971, 1275, 1493, 2323
MspR9I CCNGG 6 cut(s) 115, 210, 543, 618, 972, 1493
Mva1269I GAATGC 3 cut(s) 1327, 2280, 2452
MvaI CCWGG 4 cut(s) 115, 210, 543, 618
MwoI GCNNNNNNNGC 5 cut(s) 826, 930, 1115, 1322, 1388
NciI CCSGG 2 cut(s) 972, 1493
NcoI CCATGG 1 cut(s) 1996
NdeI CATATG 3 cut(s) 439, 1192, 2041
NdeII GATC 7 cut(s) 128, 282, 738, 1099, 1282, 1510, 1918
NlaIV GGNNCC 3 cut(s) 656, 916, 2003
NmuCI GTSAC 4 cut(s) 603, 893, 1195, 2052
NsiI ATGCAT 1 cut(s) 2282
NspI RCATGY 3 cut(s) 1360, 2195, 2204
PaeI GCATGC 1 cut(s) 1360
PaeR7I CTCGAG 1 cut(s) 1894
PciI ACATGT 1 cut(s) 2191
PctI GAATGC 3 cut(s) 1327, 2280, 2452
PdmI GAANNNNTTC 2 cut(s) 1177, 2410
PfeI GAWTC 7 cut(s) 495, 562, 1341, 1708, 2079, 2237, 2312
PfoI TCCNGGA 1 cut(s) 208
PkrI GCNGC 7 cut(s) 185, 1120, 1327, 1531, 1643, 2388, 2425
PleI GAGTC 3 cut(s) 1049, 1784, 1886
PpsI GAGTC 3 cut(s) 1049, 1784, 1886
PpuMI RGGWCCY 2 cut(s) 10, 1818
PscI ACATGT 1 cut(s) 2191
PshAI GACNNNNGTC 1 cut(s) 1448
PsiI TTATAA 4 cut(s) 366, 687, 777, 1590
Psp124BI GAGCTC 1 cut(s) 2491
Psp5II RGGWCCY 2 cut(s) 10, 1818
Psp6I CCWGG 4 cut(s) 113, 208, 541, 616
PspGI CCWGG 4 cut(s) 113, 208, 541, 616
PspN4I GGNNCC 3 cut(s) 656, 916, 2003
PspPI GGNCC 6 cut(s) 10, 655, 1390, 1818, 2002, 2320
PspPPI RGGWCCY 2 cut(s) 10, 1818
PstI CTGCAG 1 cut(s) 397
PsuI RGATCY 3 cut(s) 1099, 1282, 1918
RsaI GTAC 4 cut(s) 444, 1144, 1374, 1398
RsaNI GTAC 4 cut(s) 443, 1143, 1373, 1397
RseI CAYNNNNRTG 1 cut(s) 912
SacI GAGCTC 1 cut(s) 2491
SaqAI TTAA 7 cut(s) 536, 590, 1221, 1428, 1554, 1901, 2460
SatI GCNGC 7 cut(s) 184, 1119, 1326, 1530, 1642, 2387, 2424
Sau3AI GATC 7 cut(s) 128, 282, 738, 1099, 1282, 1510, 1918
Sau96I GGNCC 6 cut(s) 10, 655, 1390, 1818, 2002, 2320
ScaI AGTACT 2 cut(s) 1374, 1398
SchI GAGTC 3 cut(s) 1050, 1784, 1886
ScrFI CCNGG 6 cut(s) 115, 210, 543, 618, 972, 1493
SduI GDGCHC 1 cut(s) 2491
SfaNI GCATC 6 cut(s) 388, 835, 1118, 1743, 2267, 2375
SfcI CTRYAG 2 cut(s) 393, 525
Sfr274I CTCGAG 1 cut(s) 1894
SinI GGWCC 4 cut(s) 10, 1818, 2002, 2320
SlaI CTCGAG 1 cut(s) 1894
SmiMI CAYNNNNRTG 1 cut(s) 912
SmlI CTYRAG 6 cut(s) 124, 143, 1427, 1472, 1894, 2434
SmoI CTYRAG 6 cut(s) 124, 143, 1427, 1472, 1894, 2434
SpeI ACTAGT 1 cut(s) 1522
SphI GCATGC 1 cut(s) 1360
SsiI CCGC 2 cut(s) 226, 1326
SspI AATATT 2 cut(s) 1936, 1992
SstI GAGCTC 1 cut(s) 2491
StyD4I CCNGG 6 cut(s) 113, 208, 541, 616, 970, 1491
StyI CCWWGG 2 cut(s) 725, 1996
TaaI ACNGT 5 cut(s) 274, 941, 1377, 1635, 2295
TaqI TCGA 8 cut(s) 131, 737, 1112, 1308, 1509, 1764, 1895, 2491
TatI WGTACW 3 cut(s) 442, 1372, 1396
TauI GCSGC 1 cut(s) 1328
TfiI GAWTC 7 cut(s) 495, 562, 1341, 1708, 2079, 2237, 2312
Tru1I TTAA 7 cut(s) 536, 590, 1221, 1428, 1554, 1901, 2460
Tru9I TTAA 7 cut(s) 536, 590, 1221, 1428, 1554, 1901, 2460
TscAI CASTG 3 cut(s) 279, 946, 2073
TseFI GTSAC 4 cut(s) 603, 893, 1195, 2052
TseI GCWGC 6 cut(s) 183, 1118, 1529, 1641, 2386, 2423
Tsp45I GTSAC 4 cut(s) 603, 893, 1195, 2052
TspGWI ACGGA 1 cut(s) 507
TspRI CASTG 3 cut(s) 279, 946, 2073
Vha464I CTTAAG 1 cut(s) 1427
VpaK11BI GGWCC 4 cut(s) 10, 1818, 2002, 2320
XapI RAATTY 4 cut(s) 214, 756, 1664, 2410
XbaI TCTAGA 1 cut(s) 1942
XceI RCATGY 3 cut(s) 1360, 2195, 2204
XhoI CTCGAG 1 cut(s) 1894
XmiI GTMKAC 1 cut(s) 967
XmnI GAANNNNTTC 2 cut(s) 1177, 2410
ZrmI AGTACT 2 cut(s) 1374, 1398
Zsp2I ATGCAT 1 cut(s) 2282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.