MD05G1327800.v1.1

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000112
Physical Location & Seq
Forward (+)
45337750 .. 45340016
2267 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1327800.v1.1.491

Sequence Viewer

Length: 1284 bp
ATGAAAGTGGCACAAGTTAAAGATCCAGACACAACGAAAGCAGCACGGCTGGAAAAAGGTATTAGTGTCAAAGAGTGTAAGCAGGTGTGCTTAAGCAATTGTTCTTGCACTGCATATATGGTCATAGAATCTGAAGGGCCCAGTGATTGCTTGACATGGTATGGTGAGTTGCTGGACATTTTAGTGCACACACAGGGAGGGCGAGATCTACATGTACGTGTGGACAAAATCGAATTAGCTGAAAATTCCAGAAACTCGAAAGGTTTTCTTAGAAGGAGGGGTATGCTGGCTGTTCTGATGTTGTCTGTTCTTCTAGCATTGGTACTGATGGTTGCGTTGACCTGTTGGAGGATTAGGAAACAGAGGAAGACAAAAGTTGAGATCGAAGAAACTAGGAGACATCCCGAATGGCAATTTTTCCATCTGAGAACGATAATTGCAGCTACAAACAACTTCTCTCCAGTCCAAAAACTTGGCCAAGGTGGTTTTGGCACTGTTTATAAGGGTGTGTTACGAAATAATCAGAATATTGCTGTAAAAAGATTATCCAGAACTTCAGGACAAGGAGTTGAAGAATTCAAAAATGAAGTTGCGCTGATAGCGAGATTTCAACACAGGAATCTTGTGAAACTTTTAGGCTGTTGCATAAAGGGAGAAGAAAGGATGTTAGTCCTGGAATACTTGCCGAACAAAAGCTTGGACTACTTTCTTTTCGATCACACGAAAAAGTCCTTGCTGGATTGGCAAAAGCGATTCGAAATCATCAATGGGGTTGCTCGTGGGGTTTTGTATCTTCACCAGGATTCAAGACTTAGGATTATTCACAGGGATCTAAAAACCAGCAATGTCCTTCTAGACGCTGAGATGAACCCAAAAATATCAGATTTCGGCATGGCAAGAATCTTCCATGGGGACCAACTCCAGGATACGACCAACAAAGTTGTCGGAACATATGGCTATATGTCACTGGAGTATGCAGTTTGGCAGTTGTGGAGTGAAGACAGAGCCTTAGAAATTGTGGATTCGTCATTGGAGTCATACGCGCCCGACGAAGCCATGAGATGCATTCAGGTCGGGCTCTTGTGTGTCGAAGAAGAGTCGAAGAACAGACCATCCATGTCAGCTGTTGTTTTTATGTTGAGCGGCGAAGCATCTGCTCCATCTCCTCAGCAGCCTGCATTTGCCTTGAGAAAAAATTCATGCGGCGATGCTGTTGTTCCGTCAGTTTCCGAAGGATCGTGTTCTATCAACGATGTGACAATAACTAAATTCGAAGGTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

428

Amino Acids

48.23

Weight (kDa)

8.72

Isoelectric Point (pI)

47.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAN_2 PF08276 2 - 60 9.2e-15 PAN-like domain
Pkinase PF00069 154 - 324 3.3e-36 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 156 - 325 3.1e-36 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 501
AarI CACCTGC 1 cut(s) 73
Acc36I ACCTGC 1 cut(s) 73
AccBSI CCGCTC 1 cut(s) 1143
AccII CGCG 1 cut(s) 1043
AciI CCGC 2 cut(s) 1143, 1203
AclWI GGATC 3 cut(s) 17, 837, 1243
AcoI YGGCCR 1 cut(s) 475
AcsI RAATTY 4 cut(s) 244, 575, 1195, 1268
AcuI CTGAAG 2 cut(s) 153, 540
AfaI GTAC 2 cut(s) 216, 324
AfiI CCNNNNNNNGG 2 cut(s) 348, 922
AflII CTTAAG 1 cut(s) 91
AflIII ACRYGT 2 cut(s) 211, 217
AgsI TTSAA 4 cut(s) 572, 580, 611, 807
AjnI CCWGG 3 cut(s) 672, 798, 921
AjuI GAANNNNNNNTTGG 2 cut(s) 680, 712
AloI GAACNNNNNNTCC 2 cut(s) 1097, 1129
AluBI AGCT 4 cut(s) 239, 443, 696, 1124
AluI AGCT 4 cut(s) 239, 443, 696, 1124
Alw21I GWGCWC 1 cut(s) 189
Alw26I GTCTC 1 cut(s) 391
Alw44I GTGCAC 1 cut(s) 185
AlwI GGATC 3 cut(s) 17, 837, 1243
AoxI GGCC 2 cut(s) 137, 475
ApaI GGGCCC 1 cut(s) 141
ApaLI GTGCAC 1 cut(s) 185
ApeKI GCWGC 3 cut(s) 41, 440, 1171
ApoI RAATTY 4 cut(s) 244, 575, 1195, 1268
Asp700I GAANNNNTTC 1 cut(s) 1195
AspLEI GCGC 2 cut(s) 595, 1045
AspS9I GGNCC 3 cut(s) 137, 138, 913
AsuHPI GGTGA 2 cut(s) 176, 788
AsuII TTCGAA 2 cut(s) 756, 1272
AvaII GGWCC 1 cut(s) 913
BaeGI GKGCMC 2 cut(s) 141, 189
BalI TGGCCA 1 cut(s) 477
BanII GRGCYC 2 cut(s) 141, 1080
BauI CACGAG 1 cut(s) 777
BbsI GAAGAC 2 cut(s) 374, 1005
Bbv12I GWGCWC 1 cut(s) 189
BbvCI CCTCAGC 1 cut(s) 1167
BbvI GCAGC 3 cut(s) 53, 452, 1183
BccI CCATC 4 cut(s) 322, 429, 1120, 1168
BceAI ACGGC 1 cut(s) 62
BcgI CGANNNNNNTGC 4 cut(s) 1054, 1088, 1136, 1170
BciT130I CCWGG 3 cut(s) 674, 800, 923
BciVI GTATCC 1 cut(s) 919
BcoDI GTCTC 1 cut(s) 391
BfaI CTAG 3 cut(s) 314, 393, 854
BfrI CTTAAG 1 cut(s) 91
BfuAI ACCTGC 1 cut(s) 73
BfuI GTATCC 1 cut(s) 919
BglII AGATCT 1 cut(s) 205
BisI GCNGC 5 cut(s) 42, 441, 1144, 1172, 1204
BlsI GCNGC 5 cut(s) 43, 442, 1145, 1173, 1205
Bme1390I CCNGG 3 cut(s) 674, 800, 923
Bme18I GGWCC 1 cut(s) 913
BmgT120I GGNCC 3 cut(s) 137, 138, 913
BmiI GGNNCC 2 cut(s) 139, 914
BmrFI CCNGG 3 cut(s) 674, 800, 923
BmrI ACTGGG 1 cut(s) 135
BmsI GCATC 3 cut(s) 1052, 1160, 1198
BmuI ACTGGG 1 cut(s) 135
BpiI GAAGAC 2 cut(s) 374, 1005
BpmI CTGGAG 3 cut(s) 444, 905, 989
Bpu10I CCTNAGC 1 cut(s) 1167
Bpu14I TTCGAA 2 cut(s) 756, 1272
BpuEI CTTGAG 1 cut(s) 1207
BsaAI YACGTR 1 cut(s) 218
BsaJI CCNNGG 2 cut(s) 478, 907
Bsc4I CCNNNNNNNGG 2 cut(s) 348, 922
Bse1I ACTGG 3 cut(s) 141, 461, 972
Bse3DI GCAATG 1 cut(s) 850
BseBI CCWGG 3 cut(s) 674, 800, 923
BseDI CCNNGG 2 cut(s) 478, 907
BseGI GGATG 3 cut(s) 400, 669, 1112
BseLI CCNNNNNNNGG 2 cut(s) 348, 922
BseMI GCAATG 1 cut(s) 850
BseMII CTCAG 3 cut(s) 416, 852, 1181
BseNI ACTGG 3 cut(s) 141, 461, 972
BseRI GAGGAG 1 cut(s) 1155
BseSI GKGCMC 2 cut(s) 141, 189
BseXI GCAGC 3 cut(s) 53, 452, 1183
Bsh1236I CGCG 1 cut(s) 1043
BshFI GGCC 2 cut(s) 139, 477
BsiHKAI GWGCWC 1 cut(s) 189
BslFI GGGAC 1 cut(s) 926
BslI CCNNNNNNNGG 2 cut(s) 348, 922
BsmAI GTCTC 1 cut(s) 391
BsmFI GGGAC 1 cut(s) 926
BsmI GAATGC 1 cut(s) 1065
BsnI GGCC 2 cut(s) 139, 477
Bsp119I TTCGAA 2 cut(s) 756, 1272
Bsp120I GGGCCC 1 cut(s) 137
Bsp1286I GDGCHC 3 cut(s) 141, 189, 1080
Bsp143I GATC 6 cut(s) 22, 205, 381, 715, 829, 1235
Bsp19I CCATGG 1 cut(s) 907
BspACI CCGC 2 cut(s) 1143, 1203
BspANI GGCC 2 cut(s) 139, 477
BspCNI CTCAG 3 cut(s) 417, 853, 1180
BspFNI CGCG 1 cut(s) 1043
BspLI GGNNCC 2 cut(s) 139, 914
BspMI ACCTGC 1 cut(s) 73
BspPI GGATC 3 cut(s) 17, 837, 1243
BspT104I TTCGAA 2 cut(s) 756, 1272
BspTI CTTAAG 1 cut(s) 91
BsrBI CCGCTC 1 cut(s) 1143
BsrDI GCAATG 1 cut(s) 850
BsrI ACTGG 3 cut(s) 141, 461, 972
BssECI CCNNGG 2 cut(s) 478, 907
BssMI GATC 6 cut(s) 22, 205, 381, 715, 829, 1235
BssSI CACGAG 1 cut(s) 777
BssT1I CCWWGG 2 cut(s) 478, 907
Bst2BI CACGAG 1 cut(s) 777
Bst2UI CCWGG 3 cut(s) 674, 800, 923
Bst4CI ACNGT 1 cut(s) 496
Bst6I CTCTTC 1 cut(s) 1089
BstAFI CTTAAG 1 cut(s) 91
BstBAI YACGTR 1 cut(s) 218
BstBI TTCGAA 2 cut(s) 756, 1272
BstC8I GCNNGC 2 cut(s) 288, 1176
BstDEI CTNAG 6 cut(s) 269, 425, 812, 861, 1009, 1167
BstDSI CCRYGG 1 cut(s) 907
BstENI CCTNNNNNAGG 1 cut(s) 346
BstF5I GGATG 3 cut(s) 400, 669, 1112
BstFNI CGCG 1 cut(s) 1043
BstHHI GCGC 2 cut(s) 595, 1045
BstKTI GATC 6 cut(s) 25, 208, 384, 718, 832, 1238
BstMAI GTCTC 1 cut(s) 391
BstMBI GATC 6 cut(s) 22, 205, 381, 715, 829, 1235
BstMWI GCNNNNNNNGC 2 cut(s) 599, 742
BstNI CCWGG 3 cut(s) 674, 800, 923
BstNSI RCATGY 1 cut(s) 215
BstSCI CCNGG 3 cut(s) 672, 798, 921
BstSLI GKGCMC 2 cut(s) 141, 189
BstUI CGCG 1 cut(s) 1043
BstV1I GCAGC 3 cut(s) 53, 452, 1183
BstV2I GAAGAC 2 cut(s) 374, 1005
BstX2I RGATCY 3 cut(s) 22, 205, 829
BstXI CCANNNNNNTGG 1 cut(s) 473
BstYI RGATCY 3 cut(s) 22, 205, 829
BsuI GTATCC 1 cut(s) 919
BsuRI GGCC 2 cut(s) 139, 477
BtgI CCRYGG 1 cut(s) 907
BtgZI GCGATG 1 cut(s) 1221
BtsCI GGATG 3 cut(s) 400, 669, 1112
BtsI GCAGTG 1 cut(s) 108
BtsIMutI CAGTG 4 cut(s) 108, 148, 492, 965
BveI ACCTGC 1 cut(s) 73
Cac8I GCNNGC 2 cut(s) 288, 1176
CfoI GCGC 2 cut(s) 595, 1045
Cfr13I GGNCC 3 cut(s) 137, 138, 913
CseI GACGC 1 cut(s) 866
Csp6I GTAC 2 cut(s) 215, 323
CviAII CATG 7 cut(s) 156, 212, 892, 908, 1057, 1117, 1200
CviQI GTAC 2 cut(s) 215, 323
DdeI CTNAG 6 cut(s) 269, 425, 812, 861, 1009, 1167
DpnI GATC 6 cut(s) 24, 207, 383, 717, 831, 1237
DpnII GATC 6 cut(s) 22, 205, 381, 715, 829, 1235
EaeI YGGCCR 1 cut(s) 475
Eam1104I CTCTTC 1 cut(s) 1089
EarI CTCTTC 1 cut(s) 1089
Eco130I CCWWGG 2 cut(s) 478, 907
Eco24I GRGCYC 2 cut(s) 141, 1080
Eco47I GGWCC 1 cut(s) 913
Eco57I CTGAAG 2 cut(s) 153, 540
EcoNI CCTNNNNNAGG 1 cut(s) 346
EcoO109I RGGNCCY 1 cut(s) 137
EcoRI GAATTC 1 cut(s) 575
EcoRII CCWGG 3 cut(s) 672, 798, 921
EcoT14I CCWWGG 2 cut(s) 478, 907
EcoT22I ATGCAT 1 cut(s) 1067
EcoT38I GRGCYC 2 cut(s) 141, 1080
ErhI CCWWGG 2 cut(s) 478, 907
FaeI CATG 7 cut(s) 159, 215, 895, 911, 1060, 1120, 1203
FalI AAGNNNNNCTT 2 cut(s) 252, 284
FaqI GGGAC 1 cut(s) 926
FatI CATG 7 cut(s) 155, 211, 891, 907, 1056, 1116, 1199
FauNDI CATATG 1 cut(s) 952
Fnu4HI GCNGC 5 cut(s) 42, 441, 1144, 1172, 1204
FokI GGATG 3 cut(s) 387, 676, 1099
FriOI GRGCYC 2 cut(s) 141, 1080
Fsp4HI GCNGC 5 cut(s) 42, 441, 1144, 1172, 1204
FspBI CTAG 3 cut(s) 314, 393, 854
GlaI GCGC 2 cut(s) 594, 1044
GluI GCNGC 5 cut(s) 42, 441, 1144, 1172, 1204
GsuI CTGGAG 3 cut(s) 444, 905, 989
HaeIII GGCC 2 cut(s) 139, 477
HgaI GACGC 1 cut(s) 866
HhaI GCGC 2 cut(s) 595, 1045
Hin1II CATG 7 cut(s) 159, 215, 895, 911, 1060, 1120, 1203
Hin6I GCGC 2 cut(s) 593, 1043
HinP1I GCGC 2 cut(s) 593, 1043
HincII GTYRAC 1 cut(s) 339
HindII GTYRAC 1 cut(s) 339
HindIII AAGCTT 1 cut(s) 694
HinfI GANTC 8 cut(s) 128, 619, 753, 803, 900, 1022, 1034, 1097
HphI GGTGA 2 cut(s) 176, 788
Hpy166II GTNNAC 3 cut(s) 187, 223, 339
Hpy188I TCNGA 7 cut(s) 133, 297, 426, 525, 883, 947, 1231
Hpy188III TCNNGA 7 cut(s) 26, 249, 404, 549, 558, 807, 854
Hpy8I GTNNAC 3 cut(s) 187, 223, 339
Hpy99I CGWCG 1 cut(s) 1052
HpyAV CCTTC 5 cut(s) 128, 267, 860, 1226, 1268
HpyCH4III ACNGT 1 cut(s) 496
HpyCH4IV ACGT 1 cut(s) 217
HpyCH4V TGCA 8 cut(s) 108, 113, 187, 440, 645, 977, 1065, 1178
HpyF10VI GCNNNNNNNGC 2 cut(s) 599, 742
HpyF3I CTNAG 6 cut(s) 269, 425, 812, 861, 1009, 1167
HpySE526I ACGT 1 cut(s) 217
Hsp92II CATG 7 cut(s) 159, 215, 895, 911, 1060, 1120, 1203
HspAI GCGC 2 cut(s) 593, 1043
Kzo9I GATC 6 cut(s) 22, 205, 381, 715, 829, 1235
LmnI GCTCC 1 cut(s) 1162
Lsp1109I GCAGC 3 cut(s) 53, 452, 1183
LweI GCATC 3 cut(s) 1052, 1160, 1198
MaeI CTAG 3 cut(s) 314, 393, 854
MaeII ACGT 1 cut(s) 217
MaeIII GTNAC 3 cut(s) 510, 963, 1255
MalI GATC 6 cut(s) 24, 207, 383, 717, 831, 1237
MbiI CCGCTC 1 cut(s) 1143
MboI GATC 6 cut(s) 22, 205, 381, 715, 829, 1235
MfeI CAATTG 1 cut(s) 97
MflI RGATCY 3 cut(s) 22, 205, 829
MhlI GDGCHC 3 cut(s) 141, 189, 1080
MlsI TGGCCA 1 cut(s) 477
MluCI AATT 9 cut(s) 97, 233, 244, 413, 435, 575, 1014, 1195, 1268
MluNI TGGCCA 1 cut(s) 477
MlyI GAGTC 2 cut(s) 1043, 1106
MmeI TCCRAC 2 cut(s) 326, 925
MnlI CCTC 5 cut(s) 191, 270, 342, 357, 1176
Mox20I TGGCCA 1 cut(s) 477
Mph1103I ATGCAT 1 cut(s) 1067
MroXI GAANNNNTTC 1 cut(s) 1195
MscI TGGCCA 1 cut(s) 477
MseI TTAA 2 cut(s) 18, 92
MslI CAYNNNNRTG 2 cut(s) 182, 216
Msp20I TGGCCA 1 cut(s) 477
MspA1I CMGCKG 1 cut(s) 1124
MspCI CTTAAG 1 cut(s) 91
MspR9I CCNGG 3 cut(s) 674, 800, 923
MunI CAATTG 1 cut(s) 97
Mva1269I GAATGC 1 cut(s) 1065
MvaI CCWGG 3 cut(s) 674, 800, 923
MvnI CGCG 1 cut(s) 1043
MwoI GCNNNNNNNGC 2 cut(s) 599, 742
NcoI CCATGG 1 cut(s) 907
NdeI CATATG 1 cut(s) 952
NdeII GATC 6 cut(s) 22, 205, 381, 715, 829, 1235
NlaIII CATG 7 cut(s) 159, 215, 895, 911, 1060, 1120, 1203
NlaIV GGNNCC 2 cut(s) 139, 914
NmuCI GTSAC 2 cut(s) 963, 1255
NsiI ATGCAT 1 cut(s) 1067
NspI RCATGY 1 cut(s) 215
NspV TTCGAA 2 cut(s) 756, 1272
PaqCI CACCTGC 1 cut(s) 73
PciI ACATGT 1 cut(s) 211
PcsI WCGNNNNNNNCGW 1 cut(s) 1047
PctI GAATGC 1 cut(s) 1065
PdmI GAANNNNTTC 1 cut(s) 1195
PfeI GAWTC 6 cut(s) 128, 619, 753, 803, 900, 1022
PfoI TCCNGGA 2 cut(s) 672, 921
PkrI GCNGC 5 cut(s) 43, 442, 1145, 1173, 1205
PleI GAGTC 2 cut(s) 1042, 1105
PpsI GAGTC 2 cut(s) 1042, 1105
Ppu21I YACGTR 1 cut(s) 218
PscI ACATGT 1 cut(s) 211
PsiI TTATAA 1 cut(s) 501
Psp6I CCWGG 3 cut(s) 672, 798, 921
PspGI CCWGG 3 cut(s) 672, 798, 921
PspN4I GGNNCC 2 cut(s) 139, 914
PspOMI GGGCCC 1 cut(s) 137
PspPI GGNCC 3 cut(s) 137, 138, 913
PsuI RGATCY 3 cut(s) 22, 205, 829
PvuII CAGCTG 1 cut(s) 1124
RsaI GTAC 2 cut(s) 216, 324
RsaNI GTAC 2 cut(s) 215, 323
RseI CAYNNNNRTG 2 cut(s) 182, 216
SaqAI TTAA 2 cut(s) 18, 92
SatI GCNGC 5 cut(s) 42, 441, 1144, 1172, 1204
Sau3AI GATC 6 cut(s) 22, 205, 381, 715, 829, 1235
Sau96I GGNCC 3 cut(s) 137, 138, 913
SchI GAGTC 2 cut(s) 1043, 1106
ScrFI CCNGG 3 cut(s) 674, 800, 923
SduI GDGCHC 3 cut(s) 141, 189, 1080
SfaNI GCATC 3 cut(s) 1052, 1160, 1198
SfuI TTCGAA 2 cut(s) 756, 1272
SinI GGWCC 1 cut(s) 913
SmiMI CAYNNNNRTG 2 cut(s) 182, 216
SmlI CTYRAG 2 cut(s) 91, 1186
SmoI CTYRAG 2 cut(s) 91, 1186
Sse9I AATT 9 cut(s) 97, 233, 244, 413, 435, 575, 1014, 1195, 1268
SsiI CCGC 2 cut(s) 1143, 1203
SspI AATATT 1 cut(s) 529
SspMI CTAG 3 cut(s) 314, 393, 854
StyD4I CCNGG 3 cut(s) 672, 798, 921
StyI CCWWGG 2 cut(s) 478, 907
TaaI ACNGT 1 cut(s) 496
TaiI ACGT 1 cut(s) 220
TaqI TCGA 9 cut(s) 231, 257, 384, 714, 756, 1089, 1100, 1272, 1279
TasI AATT 9 cut(s) 97, 233, 244, 413, 435, 575, 1014, 1195, 1268
TauI GCSGC 2 cut(s) 1146, 1206
TfiI GAWTC 6 cut(s) 128, 619, 753, 803, 900, 1022
Tru1I TTAA 2 cut(s) 18, 92
Tru9I TTAA 2 cut(s) 18, 92
TscAI CASTG 4 cut(s) 115, 148, 499, 972
TseFI GTSAC 2 cut(s) 963, 1255
TseI GCWGC 3 cut(s) 41, 440, 1171
Tsp45I GTSAC 2 cut(s) 963, 1255
TspDTI ATGAA 4 cut(s) 17, 600, 881, 1188
TspGWI ACGGA 1 cut(s) 1209
TspRI CASTG 4 cut(s) 115, 148, 499, 972
Vha464I CTTAAG 1 cut(s) 91
VneI GTGCAC 1 cut(s) 185
VpaK11BI GGWCC 1 cut(s) 913
XagI CCTNNNNNAGG 1 cut(s) 346
XapI RAATTY 4 cut(s) 244, 575, 1195, 1268
XbaI TCTAGA 1 cut(s) 853
XceI RCATGY 1 cut(s) 215
XcmI CCANNNNNNNNNTGG 1 cut(s) 485
XmnI GAANNNNTTC 1 cut(s) 1195
XspI CTAG 3 cut(s) 314, 393, 854
Zsp2I ATGCAT 1 cut(s) 1067
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.