Rw3G018840

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000112
Physical Location & Seq
Forward (+)
21116612 .. 21119976
3365 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G018840.1

Sequence Viewer

Length: 2361 bp
ATGGTCAACCTGAAGCTCAAACTACAAGACTTCAGAGTTCAGAATGCGCGTAAACAGCTAGTAGAATTGTCAACAACAGAGACACTGGAAATGGACACCATCAGATGGAATGAACAACAATTGAAGGATGTTGATGGTGAAGTTTTGGTGTCCAAAGAAAGCAACTTTGAGTTGGGTTTCTTCAGCCCCGGCGATTCTAGCCACCGGTATGGGGAATTAGCTCTTTATGCTTATAACATGGAGAGCATTCCTGTATGGTCTACAAATGTATCACAGTCGGTCCAAAACACAGACACCTTATATAGATATGTCCAGCTTTTGGATACTGGAAACTTAATTCTGTTCCGGGATGGTAGAAATGGAAGCTTGATTTTATGGCAAAGTTTTGATTATCCTGCAGATACTCTAATCCCAGGTATGAAAGTTGGGTTGAATTGGAAAAAAGGTCTAGATTGGGTTTTAACATCTTGGAAGTCACAAGATGACCCTGGAAAGGGGGACTATGCCTTTAGGGTCGCTCCGAATCACAATGCTACTCCCCAGTATTTTGTCTACAAGGGTTTCACTAAGTATTGGCGAATGCATGCAGGGCCATGGCCTAATTTTGTTAGTAATGCCAACGAAATGTATAATACCCTGCCTTATAAGAGCGGTGCTATTTCAATAGTAAGGATGAAGGATTCCGGGTTACTTCAGCACCTAACATGGAATGATACTGACCATCAGTGGGTGGAAATGTGGTCTTCGCCGAAATACCCTTGTGACTGGTATGGACATTGTGGTGCCAACAGCAGATGTAGGCCTGACAATATTAATTTATTTGAGTGTGAGTGTATTCCAGGGTATGAGCCAAAATCTGTAGATGGTTGGAATCGGAGAGATGGAACAGACGGATGTGTGAGTAAACGAGTAAGAGTGTCCAAGTGTAGGAATGGAGAAGGGTTTGTGAAGGTGGCAAGGGTAAAGGATCCAGACACATCGAATGTAGCAAGGTTGAGAACAAGTATGAGTGCCAAAGAGTGTGAGCAGGAGTGCTTGAAAAATTGTTCTTGCACAGCGTATATGAGCATTACACTGGAAAGGGTTGCCAGTTGCTTGACATGGTATGATGACTTGATGGACATGTTAGTTTACACAGGGGTCGGACGAGATCTATATGTTCGTGTGGATAAAATCTCATTAGCTGAGGCCAGAAACTCACAAGGTTTCTTCAGGAGTGGCAAGGTCCTTATTCCAGTACTAACTGCCCTGGTGGCACTACTACTAATCATTATACTTTCCTTGTGGTTGCTTAAGAAAAAGAGGAAGACAAGAGACTATCTGGATGCGGAGGAACTTGAGGAAACTAAGAGACACCCGGAATTGCAATTTTTCGATCTTAACACACTGATAGCAGCCACAGACAACTTCTCTGATGCCAATAAACTTGGCCAAGGTGGTTTTGGCAGTGTTTATAAGGGCGAATTACCAAACGAACAAAAAGTTGCTATAAAAAGATTGTCTAGAACTTCAGGACAGGGAATCGAAGAATTCAAAAACGAAGTTGCACTAATAGCCAGACTTCAGCACAGGAATCTTGTGAAACTTTTAGGCTGCTGTATAAAGGGAGAAGAAAGGATATTGGTTCTGGAATACATGCCGAACAAAAGCTTGGACTCCTTTCTTTTTGATAACACAAGGAGGTCACTCTTAAATTGGGAAAAGCGTTTTGAAATTATCAATGGGGTTGCTCGTGGGGTTCTATATCTTCATCGGGACTCAAGATTGAGGGTTATCCATAGGGATCTAAAACCCGGTAATGTCCTTCTAGATGCCGAGTTAGACCCAAAAATTTCTGATTTCGGCATGGCAAGAATATTTCAAGGGGACCAACTCCTGGAAAAGACAAACAGAGTTGTCGGAACATATGGGTACATGTCACCGGAATATGCAGTATTTGGGAGATTTTCTACGAAATCGGATGTGTTTAGTTTTGGGATCATACTATTGGAGATTATTAGTGGCAAGAGAAACAATGGTTGTTTTGAGATGCATTATTCCATAAACTTGATAGGACATGTATGGCAACTTTGGGTAGAAGACAGAGTCTTGGAAATTGTGGATTCATCACTAGAGATTTATCAGCTTGATGAAGTCTTGAGATGCATTCAAGTGGGGCTTTTGTGCGTGCAAGAAGATTTGAAGGACCGACCAGCCATGTCAGCCGTCGTGTTCATGTTGAGTGGTGAAACATCTCTTCCATCTCCTAAGCAGCCTGCATTTGTCTTCAGAACAAGTTCCAGCATTGTTGATGATCCCTTCATTCCAAATGAATCATATTCTAGTAATAGTTTGACAATAACTAACATGGAAGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

786

Amino Acids

89.79

Weight (kDa)

6.51

Isoelectric Point (pI)

41.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 71 - 158 3.4e-20 D-mannose binding lectin
S_locus_glycop PF00954 201 - 286 7.4e-15 S-locus glycoprotein domain
PAN_2 PF08276 309 - 375 3.6e-16 PAN-like domain
Pkinase PF00069 471 - 670 6.8e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 474 - 737 2e-48 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 743 - 786 3.5e-06 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 234, 645, 1455
AccB1I GGYRCC 1 cut(s) 782
AccB7I CCANNNNNTGG 3 cut(s) 105, 319, 1876
AccBSI CCGCTC 1 cut(s) 651
AccI GTMKAC 2 cut(s) 260, 552
AccII CGCG 1 cut(s) 49
AciI CCGC 2 cut(s) 651, 1328
AclWI GGATC 5 cut(s) 962, 975, 1791, 1985, 2288
AcoI YGGCCR 1 cut(s) 1429
AcsI RAATTY 2 cut(s) 1529, 1830
AcuI CTGAAG 8 cut(s) 16, 32, 166, 677, 1195, 1494, 1547, 2251
AfaI GTAC 2 cut(s) 1239, 1913
AfiI CCNNNNNNNGG 8 cut(s) 105, 211, 319, 493, 494, 727, 927, 1876
AflII CTTAAG 1 cut(s) 1292
AflIII ACRYGT 3 cut(s) 1122, 1914, 2056
AgeI ACCGGT 1 cut(s) 204
AgsI TTSAA 9 cut(s) 124, 433, 663, 1039, 1534, 1712, 1862, 2150, 2182
AjnI CCWGG 5 cut(s) 412, 487, 838, 1248, 1875
AjuI GAANNNNNNNTTGG 2 cut(s) 1634, 1666
AluBI AGCT 9 cut(s) 16, 58, 221, 316, 366, 1184, 1650, 2125, 2354
AluI AGCT 9 cut(s) 16, 58, 221, 316, 366, 1184, 1650, 2125, 2354
Alw26I GTCTC 3 cut(s) 74, 1308, 1345
AlwI GGATC 5 cut(s) 962, 975, 1791, 1985, 2288
AoxI GGCC 5 cut(s) 590, 596, 800, 1188, 1429
ApeKI GCWGC 3 cut(s) 1394, 1593, 2251
ApoI RAATTY 2 cut(s) 1529, 1830
AseI ATTAAT 1 cut(s) 813
AsiGI ACCGGT 1 cut(s) 204
Asp700I GAANNNNTTC 1 cut(s) 2275
AspLEI GCGC 1 cut(s) 49
AspS9I GGNCC 5 cut(s) 280, 590, 1225, 1867, 2185
AsuC2I CCSGG 5 cut(s) 189, 347, 685, 1358, 1794
AsuHPI GGTGA 3 cut(s) 149, 1911, 2237
AvaII GGWCC 4 cut(s) 280, 1225, 1867, 2185
BaeI ACNNNNGTAYC 2 cut(s) 252, 285
BalI TGGCCA 1 cut(s) 1431
BamHI GGATCC 1 cut(s) 967
BanI GGYRCC 1 cut(s) 782
BauI CACGAG 1 cut(s) 1731
BbsI GAAGAC 4 cut(s) 735, 1313, 2085, 2257
BbvCI CCTCAGC 1 cut(s) 1185
BbvI GCAGC 3 cut(s) 1406, 1580, 2263
BccI CCATC 9 cut(s) 99, 107, 128, 344, 729, 857, 875, 1111, 2248
BceAI ACGGC 1 cut(s) 2189
BciT130I CCWGG 5 cut(s) 414, 489, 840, 1250, 1877
BciVI GTATCC 1 cut(s) 316
BcnI CCSGG 5 cut(s) 189, 347, 685, 1358, 1794
BcoDI GTCTC 3 cut(s) 74, 1308, 1345
BfaI CTAG 7 cut(s) 59, 198, 449, 1503, 1808, 2111, 2322
BfmI CTRYAG 2 cut(s) 396, 858
BfrI CTTAAG 1 cut(s) 1292
BfuI GTATCC 1 cut(s) 316
BglI GCCNNNNNGGC 1 cut(s) 1253
BglII AGATCT 1 cut(s) 1150
BisI GCNGC 3 cut(s) 1395, 1594, 2252
BlsI GCNGC 3 cut(s) 1396, 1595, 2253
BmcAI AGTACT 1 cut(s) 1239
Bme18I GGWCC 4 cut(s) 280, 1225, 1867, 2185
BmgT120I GGNCC 5 cut(s) 280, 590, 1225, 1867, 2185
BmiI GGNNCC 3 cut(s) 784, 969, 1868
BmrI ACTGGG 1 cut(s) 535
BmsI GCATC 5 cut(s) 1315, 1405, 1801, 2019, 2132
BmuI ACTGGG 1 cut(s) 535
BpiI GAAGAC 4 cut(s) 735, 1313, 2085, 2257
Bpu10I CCTNAGC 2 cut(s) 1185, 2247
BpuEI CTTGAG 3 cut(s) 1358, 1744, 2158
BpuMI CCSGG 5 cut(s) 189, 347, 685, 1358, 1794
BsaJI CCNNGG 7 cut(s) 187, 412, 487, 593, 839, 1248, 1432
BsaWI WCCGGW 2 cut(s) 204, 1921
Bsc4I CCNNNNNNNGG 8 cut(s) 105, 211, 319, 493, 494, 727, 927, 1876
Bse118I RCCGGY 1 cut(s) 204
Bse1I ACTGG 7 cut(s) 90, 331, 541, 770, 1080, 1089, 1235
BseBI CCWGG 5 cut(s) 414, 489, 840, 1250, 1877
BseDI CCNNGG 7 cut(s) 187, 412, 487, 593, 839, 1248, 1432
BseGI GGATG 6 cut(s) 133, 355, 678, 899, 1330, 1966
BseLI CCNNNNNNNGG 8 cut(s) 105, 211, 319, 493, 494, 727, 927, 1876
BseMII CTCAG 1 cut(s) 1176
BseNI ACTGG 7 cut(s) 90, 331, 541, 770, 1080, 1089, 1235
BseXI GCAGC 3 cut(s) 1406, 1580, 2263
Bsh1236I CGCG 1 cut(s) 49
BshFI GGCC 5 cut(s) 592, 598, 802, 1190, 1431
BshNI GGYRCC 1 cut(s) 782
BshTI ACCGGT 1 cut(s) 204
BsiSI CCGG 7 cut(s) 189, 205, 346, 684, 1358, 1794, 1922
BslFI GGGAC 3 cut(s) 512, 1769, 1880
BslI CCNNNNNNNGG 8 cut(s) 105, 211, 319, 493, 494, 727, 927, 1876
BsmAI GTCTC 3 cut(s) 74, 1308, 1345
BsmFI GGGAC 3 cut(s) 512, 1769, 1880
BsmI GAATGC 4 cut(s) 49, 246, 585, 2145
BsnI GGCC 5 cut(s) 592, 598, 802, 1190, 1431
Bsp143I GATC 6 cut(s) 967, 1150, 1375, 1783, 1977, 2293
Bsp19I CCATGG 1 cut(s) 593
BspACI CCGC 2 cut(s) 651, 1328
BspANI GGCC 5 cut(s) 592, 598, 802, 1190, 1431
BspCNI CTCAG 1 cut(s) 1177
BspFNI CGCG 1 cut(s) 49
BspLI GGNNCC 3 cut(s) 784, 969, 1868
BspMAI CTGCAG 1 cut(s) 400
BspPI GGATC 5 cut(s) 962, 975, 1791, 1985, 2288
BspT107I GGYRCC 1 cut(s) 782
BspTI CTTAAG 1 cut(s) 1292
BsrBI CCGCTC 1 cut(s) 651
BsrFI RCCGGY 1 cut(s) 204
BsrI ACTGG 7 cut(s) 90, 331, 541, 770, 1080, 1089, 1235
BssAI RCCGGY 1 cut(s) 204
BssECI CCNNGG 7 cut(s) 187, 412, 487, 593, 839, 1248, 1432
BssMI GATC 6 cut(s) 967, 1150, 1375, 1783, 1977, 2293
BssSI CACGAG 1 cut(s) 1731
BssT1I CCWWGG 2 cut(s) 593, 1432
Bst2BI CACGAG 1 cut(s) 1731
Bst2UI CCWGG 5 cut(s) 414, 489, 840, 1250, 1877
Bst4CI ACNGT 1 cut(s) 276
Bst6I CTCTTC 1 cut(s) 2241
BstAFI CTTAAG 1 cut(s) 1292
BstC8I GCNNGC 3 cut(s) 585, 2168, 2256
BstDEI CTNAG 4 cut(s) 567, 1185, 1347, 2247
BstDSI CCRYGG 1 cut(s) 593
BstF5I GGATG 6 cut(s) 133, 355, 678, 899, 1330, 1966
BstFNI CGCG 1 cut(s) 49
BstHHI GCGC 1 cut(s) 49
BstKTI GATC 6 cut(s) 970, 1153, 1378, 1786, 1980, 2296
BstMAI GTCTC 3 cut(s) 74, 1308, 1345
BstMBI GATC 6 cut(s) 967, 1150, 1375, 1783, 1977, 2293
BstMWI GCNNNNNNNGC 7 cut(s) 55, 198, 227, 589, 1253, 1553, 2201
BstNI CCWGG 5 cut(s) 414, 489, 840, 1250, 1877
BstNSI RCATGY 5 cut(s) 587, 1126, 1639, 1918, 2060
BstSFI CTRYAG 2 cut(s) 396, 858
BstUI CGCG 1 cut(s) 49
BstV1I GCAGC 3 cut(s) 1406, 1580, 2263
BstV2I GAAGAC 4 cut(s) 735, 1313, 2085, 2257
BstX2I RGATCY 3 cut(s) 967, 1150, 1783
BstXI CCANNNNNNTGG 1 cut(s) 209
BstYI RGATCY 3 cut(s) 967, 1150, 1783
BsuI GTATCC 1 cut(s) 316
BsuRI GGCC 5 cut(s) 592, 598, 802, 1190, 1431
BtgI CCRYGG 1 cut(s) 593
BtsCI GGATG 6 cut(s) 133, 355, 678, 899, 1330, 1966
BtsI GCAGTG 1 cut(s) 1453
BtsIMutI CAGTG 5 cut(s) 83, 731, 1073, 1385, 1453
Cac8I GCNNGC 3 cut(s) 585, 2168, 2256
CfoI GCGC 1 cut(s) 49
Cfr10I RCCGGY 1 cut(s) 204
Cfr13I GGNCC 5 cut(s) 280, 590, 1225, 1867, 2185
Csp6I GTAC 2 cut(s) 1238, 1912
CspAI ACCGGT 1 cut(s) 204
CviQI GTAC 2 cut(s) 1238, 1912
DdeI CTNAG 4 cut(s) 567, 1185, 1347, 2247
DpnI GATC 6 cut(s) 969, 1152, 1377, 1785, 1979, 2295
DpnII GATC 6 cut(s) 967, 1150, 1375, 1783, 1977, 2293
EaeI YGGCCR 1 cut(s) 1429
Eam1104I CTCTTC 1 cut(s) 2241
EarI CTCTTC 1 cut(s) 2241
Eco130I CCWWGG 2 cut(s) 593, 1432
Eco147I AGGCCT 1 cut(s) 802
Eco47I GGWCC 4 cut(s) 280, 1225, 1867, 2185
Eco57I CTGAAG 8 cut(s) 16, 32, 166, 677, 1195, 1494, 1547, 2251
EcoO109I RGGNCCY 1 cut(s) 1225
EcoRI GAATTC 1 cut(s) 1529
EcoRII CCWGG 5 cut(s) 412, 487, 838, 1248, 1875
EcoT14I CCWWGG 2 cut(s) 593, 1432
EcoT22I ATGCAT 3 cut(s) 585, 2034, 2147
ErhI CCWWGG 2 cut(s) 593, 1432
FalI AAGNNNNNCTT 2 cut(s) 2142, 2174
FaqI GGGAC 3 cut(s) 512, 1769, 1880
FauNDI CATATG 1 cut(s) 1906
FblI GTMKAC 2 cut(s) 260, 552
Fnu4HI GCNGC 3 cut(s) 1395, 1594, 2252
FokI GGATG 6 cut(s) 140, 362, 685, 906, 1337, 1973
Fsp4HI GCNGC 3 cut(s) 1395, 1594, 2252
FspBI CTAG 7 cut(s) 59, 198, 449, 1503, 1808, 2111, 2322
GlaI GCGC 1 cut(s) 48
GluI GCNGC 3 cut(s) 1395, 1594, 2252
HaeIII GGCC 5 cut(s) 592, 598, 802, 1190, 1431
HapII CCGG 7 cut(s) 189, 205, 346, 684, 1358, 1794, 1922
HhaI GCGC 1 cut(s) 49
Hin6I GCGC 1 cut(s) 47
HinP1I GCGC 1 cut(s) 47
HincII GTYRAC 2 cut(s) 7, 72
HindII GTYRAC 2 cut(s) 7, 72
HindIII AAGCTT 2 cut(s) 364, 1648
HpaII CCGG 7 cut(s) 189, 205, 346, 684, 1358, 1794, 1922
HphI GGTGA 3 cut(s) 149, 1911, 2237
Hpy166II GTNNAC 7 cut(s) 7, 53, 72, 261, 553, 905, 1132
Hpy8I GTNNAC 7 cut(s) 7, 53, 72, 261, 553, 905, 1132
Hpy99I CGWCG 1 cut(s) 2210
HpyAV CCTTC 7 cut(s) 118, 670, 932, 943, 1814, 2176, 2308
HpyCH4III ACNGT 1 cut(s) 276
HpyF10VI GCNNNNNNNGC 7 cut(s) 55, 198, 227, 589, 1253, 1553, 2201
HpyF3I CTNAG 4 cut(s) 567, 1185, 1347, 2247
HspAI GCGC 1 cut(s) 47
Kzo9I GATC 6 cut(s) 967, 1150, 1375, 1783, 1977, 2293
LmnI GCTCC 1 cut(s) 523
Lsp1109I GCAGC 3 cut(s) 1406, 1580, 2263
LweI GCATC 5 cut(s) 1315, 1405, 1801, 2019, 2132
MaeI CTAG 7 cut(s) 59, 198, 449, 1503, 1808, 2111, 2322
MaeIII GTNAC 5 cut(s) 474, 687, 761, 1683, 1917
MalI GATC 6 cut(s) 969, 1152, 1377, 1785, 1979, 2295
MbiI CCGCTC 1 cut(s) 651
MboI GATC 6 cut(s) 967, 1150, 1375, 1783, 1977, 2293
MfeI CAATTG 1 cut(s) 119
MflI RGATCY 3 cut(s) 967, 1150, 1783
MlsI TGGCCA 1 cut(s) 1431
MluNI TGGCCA 1 cut(s) 1431
MlyI GAGTC 3 cut(s) 1649, 1751, 2094
MmeI TCCRAC 3 cut(s) 848, 1123, 1879
MnlI CCTC 6 cut(s) 1180, 1296, 1324, 1333, 1674, 1761
Mox20I TGGCCA 1 cut(s) 1431
Mph1103I ATGCAT 3 cut(s) 585, 2034, 2147
MroXI GAANNNNTTC 1 cut(s) 2275
MscI TGGCCA 1 cut(s) 1431
MseI TTAA 6 cut(s) 335, 461, 813, 1293, 1380, 1691
MslI CAYNNNNRTG 3 cut(s) 207, 780, 2150
Msp20I TGGCCA 1 cut(s) 1431
MspCI CTTAAG 1 cut(s) 1292
MspI CCGG 7 cut(s) 189, 205, 346, 684, 1358, 1794, 1922
MunI CAATTG 1 cut(s) 119
Mva1269I GAATGC 4 cut(s) 49, 246, 585, 2145
MvaI CCWGG 5 cut(s) 414, 489, 840, 1250, 1877
MvnI CGCG 1 cut(s) 49
MwoI GCNNNNNNNGC 7 cut(s) 55, 198, 227, 589, 1253, 1553, 2201
NciI CCSGG 5 cut(s) 189, 347, 685, 1358, 1794
NcoI CCATGG 1 cut(s) 593
NdeI CATATG 1 cut(s) 1906
NdeII GATC 6 cut(s) 967, 1150, 1375, 1783, 1977, 2293
NlaIV GGNNCC 3 cut(s) 784, 969, 1868
NmeAIII GCCGAG 1 cut(s) 1840
NmuCI GTSAC 4 cut(s) 474, 761, 1683, 1917
NsiI ATGCAT 3 cut(s) 585, 2034, 2147
NspI RCATGY 5 cut(s) 587, 1126, 1639, 1918, 2060
PaeI GCATGC 1 cut(s) 587
PceI AGGCCT 1 cut(s) 802
PciI ACATGT 3 cut(s) 1122, 1914, 2056
PctI GAATGC 4 cut(s) 49, 246, 585, 2145
PdmI GAANNNNTTC 1 cut(s) 2275
PfeI GAWTC 8 cut(s) 194, 523, 680, 871, 1521, 1573, 2102, 2312
PflFI GACNNNGTC 1 cut(s) 2084
PflMI CCANNNNNTGG 3 cut(s) 105, 319, 1876
PfoI TCCNGGA 2 cut(s) 345, 1875
PinAI ACCGGT 1 cut(s) 204
PkrI GCNGC 3 cut(s) 1396, 1595, 2253
PleI GAGTC 3 cut(s) 1649, 1751, 2093
PpsI GAGTC 3 cut(s) 1649, 1751, 2093
PpuMI RGGWCCY 1 cut(s) 1225
PscI ACATGT 3 cut(s) 1122, 1914, 2056
PshBI ATTAAT 1 cut(s) 813
PsiI TTATAA 3 cut(s) 234, 645, 1455
Psp5II RGGWCCY 1 cut(s) 1225
Psp6I CCWGG 5 cut(s) 412, 487, 838, 1248, 1875
PspGI CCWGG 5 cut(s) 412, 487, 838, 1248, 1875
PspN4I GGNNCC 3 cut(s) 784, 969, 1868
PspPI GGNCC 5 cut(s) 280, 590, 1225, 1867, 2185
PspPPI RGGWCCY 1 cut(s) 1225
PstI CTGCAG 1 cut(s) 400
PsuI RGATCY 3 cut(s) 967, 1150, 1783
PsyI GACNNNGTC 1 cut(s) 2084
RsaI GTAC 2 cut(s) 1239, 1913
RsaNI GTAC 2 cut(s) 1238, 1912
RseI CAYNNNNRTG 3 cut(s) 207, 780, 2150
SaqAI TTAA 6 cut(s) 335, 461, 813, 1293, 1380, 1691
SatI GCNGC 3 cut(s) 1395, 1594, 2252
Sau3AI GATC 6 cut(s) 967, 1150, 1375, 1783, 1977, 2293
Sau96I GGNCC 5 cut(s) 280, 590, 1225, 1867, 2185
ScaI AGTACT 1 cut(s) 1239
SchI GAGTC 3 cut(s) 1649, 1751, 2094
SfaNI GCATC 5 cut(s) 1315, 1405, 1801, 2019, 2132
SfcI CTRYAG 2 cut(s) 396, 858
SinI GGWCC 4 cut(s) 280, 1225, 1867, 2185
SmiMI CAYNNNNRTG 3 cut(s) 207, 780, 2150
SmlI CTYRAG 4 cut(s) 1292, 1337, 1759, 2137
SmoI CTYRAG 4 cut(s) 1292, 1337, 1759, 2137
SphI GCATGC 1 cut(s) 587
SseBI AGGCCT 1 cut(s) 802
SsiI CCGC 2 cut(s) 651, 1328
SspI AATATT 2 cut(s) 811, 1857
SspMI CTAG 7 cut(s) 59, 198, 449, 1503, 1808, 2111, 2322
StuI AGGCCT 1 cut(s) 802
StyI CCWWGG 2 cut(s) 593, 1432
TaaI ACNGT 1 cut(s) 276
TaqI TCGA 4 cut(s) 980, 1374, 1524, 2356
TaqII GACCGA 2 cut(s) 268, 2202
TatI WGTACW 1 cut(s) 1237
TfiI GAWTC 8 cut(s) 194, 523, 680, 871, 1521, 1573, 2102, 2312
Tru1I TTAA 6 cut(s) 335, 461, 813, 1293, 1380, 1691
Tru9I TTAA 6 cut(s) 335, 461, 813, 1293, 1380, 1691
TscAI CASTG 5 cut(s) 90, 731, 1080, 1392, 1453
TseFI GTSAC 4 cut(s) 474, 761, 1683, 1917
TseI GCWGC 3 cut(s) 1394, 1593, 2251
Tsp45I GTSAC 4 cut(s) 474, 761, 1683, 1917
TspDTI ATGAA 9 cut(s) 126, 434, 689, 1739, 2094, 2145, 2203, 2290, 2325
TspGWI ACGGA 1 cut(s) 906
TspRI CASTG 5 cut(s) 90, 731, 1080, 1392, 1453
Tth111I GACNNNGTC 1 cut(s) 2084
Van91I CCANNNNNTGG 3 cut(s) 105, 319, 1876
Vha464I CTTAAG 1 cut(s) 1292
VpaK11BI GGWCC 4 cut(s) 280, 1225, 1867, 2185
VspI ATTAAT 1 cut(s) 813
XapI RAATTY 2 cut(s) 1529, 1830
XbaI TCTAGA 3 cut(s) 448, 1502, 1807
XceI RCATGY 5 cut(s) 587, 1126, 1639, 1918, 2060
XcmI CCANNNNNNNNNTGG 1 cut(s) 1439
XmiI GTMKAC 2 cut(s) 260, 552
XmnI GAANNNNTTC 1 cut(s) 2275
XspI CTAG 7 cut(s) 59, 198, 449, 1503, 1808, 2111, 2322
ZrmI AGTACT 1 cut(s) 1239
Zsp2I ATGCAT 3 cut(s) 585, 2034, 2147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.