Rh5CG056500

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000112
Physical Location & Seq
Forward (+)
4109219 .. 4113721
4503 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG056500.1

Sequence Viewer

Length: 2481 bp
ATGAAAATCAGAAAAATGCCACTGTTTCATACCAACAAAGTTGCAAATGCCCAGAAAATGTTATTCAAAGCTCTGCTTCTCTTCCTCCTCTTCCGATTTTGCGAATCCAGGGACACCATAACATGGAACCAAGAACAACTGAAGGATGATGGTGGAGTTTTGGTGTCCAAACAGAGCAAGTTTGAATTGGGTTTCTTCAGCCCCGGAAATTCTAGCTACCGGTATGTTGGAATTTGGTATTCTCAGACTAGAGTATCTAATAAAATAGTGGTGTGGGTTGCAAACAGGAACAATCCCATCAATGATACCTCTGGTGTGCTCACAATAAACAGGTATGGAGAACTAGTCCTCTATGCTTATAACATGGAGAGCAGTCCTATTTGGTCTACTAATGTGTCGCGGTCGGTTCAAAATGTTAACACAAGCACGTTATCTGCACAGCTTTTAGATACAGGAAATCTAGTTGTGTTCCAGGATGATAATAATGAAAACTTTTTATGGCAAAGTTTTGATCATCCCACAGATACTTTAATTCCAGGTATGAAAGTTGGGATGAATTGGAAAACTGGGCAAGAATGGGTTTTAACATCTTGGAAGTCACAAGATGACCCTGGAACTGGGGACTATACCTTAAGGCTCTATCCAAATCAGATTGCATCCGCCCAATTCATTATGTACAAGGGTTCGAGTAAGTATTGGCGAAGTGATCCAGGGATAACGGGTTTGGTCACTAATCAAGAGGAAACTTATTATCCCATGAATAACACCAATGCAATTACAAGAGCAACAGTGATTGATTCTGCGGTAGAAGGCTTTACATGGGATGATGGTGGCCTTCAATGGAAGGTAGGCTTCTCTGCACCGAAGTCCCGGTGTGACTGGTATGGAGAGTGCGGTGCCAACAGCAAATGCCTCCCTGACAATGTTATTATGTTTTGGTGTGATTGTTTACCAGGGTATGTGCCTAATTCTATAAGTGATTGGAATCATAAAAATGGGTCAGGTGGATGTGTGAGTAATCGACTTGGTTTGTTGAAGTGTGGAGATGGAGACGGGTTTGTGAAGGTGGCAAGAGTTAAATATCCTGACACATCGATAGGAGCATTGTCAAAATCAGGTATGAGTGCCAAAGAGTGTGAGCAGGAGTGCCTAAGAAATTGTTCTTGCACCGCATATTTGAGCACTGAAAATGAAGGGCCTGTTGATTGCTTGACATGGTATGATGACTTGATGGACATTAAAATGTACACGGAGATTGGACAAGATCTCTACGTTCGTGTGAATGCGACCGTGTTAGCTGCAAATGCCAGAAGATCAGAAGGTTTCTTGGAAAGGAGGGGTATGCTGGCTATTCCAATACTGTCTGCTGTGCTAGCATTGGTACTAATCATTATGCTTGCATGTTGGTGGCGCAAGAAGAACAGGAACACAAAAGACTTTGTGGAGGCAGAGGAGCTTGTGGAAAAACAGAGACACCCTGAATTGCAATTTTTCGATCTTGACACAATAGTCGCAGCAACAGACCACTTCTCTCGTGTCAATGAACTTGGCCATGGTGGATTTGGCTCTGTTTATAAGGGTCAGCTACCAAACGAACAGAAAGTTGCTGTGAAAAGATTGTCCCAAACTTCAGGACAAGGGATCGAAGAATTCAAAAATGAAGTTGCACTTATAGCAAGACTTCAACACAGGAATCTTGTGAAACTTTTAGGCTGTTGCATAAAGGGAGAAGAAAGGATGTTAGTGCTAGAATACATGCCTAACAAAAGCTTGAACTCCTTTCTTTTTGATAACACAAGACGGTCCTTCTTGGATTGGGCAAAGCGCTTTGAAATTATCAACGGGATTGCTCGTGGGATTCTGTATCTTCACCAAGACTCAAGATTGAGGATCATCCATAGAGATTTGAAAACTAGCAATATTCTTCTAGATGCTGAGATGAACCCAAAAATTTCTGATTTTGGCATGGCTAGAATATTCCACAGAGATCAACTACAAGATAAGACGAGCCGAATTGTTGGAACATATGGCTACATGTCACCAGAGTACGCAGTATTTGGAAGATTTTCCATGAAATCTGATGTGTTTAGTTTTGGGATCATAATGTTGGAGATTGTAAGTGGCCGGAAAAACAATGATTCTGATTTGGAGGATCCTTCCATGAACTTGATAGGACATGTTTGGGAGCTCTGGGGAAAAGGCAGAGCCTTAGATATTGTTGATTCCACACTAAAGTCATATCAGCCTAATGAAGTCATGAGATGCATACAAGTTGCGCTCTTGTGTGTACAAGAAGATTCAAATGACCGACCTGCCATGTCAACCATTGTTTTCATGTTGAGTGGTGAATCATCTCCTCCATTGCCTAAGCATCCAGCATTTGTTTACCGAAGAGATTCCTGCATTGATGTTGATCCATTACTTCCAAACAAATCTTATTCTATAAATGACTTGACAATAACTACCATGGAAGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

826

Amino Acids

93.58

Weight (kDa)

6.5

Isoelectric Point (pI)

40.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 90 - 199 2.6e-36 D-mannose binding lectin
S_locus_glycop PF00954 244 - 323 9.8e-12 S-locus glycoprotein domain
PAN_2 PF08276 347 - 413 9.9e-18 PAN-like domain
Pkinase PF00069 511 - 776 5.2e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 513 - 778 3.2e-51 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 360, 1575
AasI GACNNNNNNGTC 1 cut(s) 1508
Acc36I ACCTGC 1 cut(s) 2320
AccB1I GGYRCC 1 cut(s) 896
AccB7I CCANNNNNTGG 1 cut(s) 123
AccI GTMKAC 1 cut(s) 386
AccII CGCG 1 cut(s) 400
AciI CCGC 5 cut(s) 400, 660, 803, 894, 1170
AclWI GGATC 7 cut(s) 701, 1649, 1898, 2105, 2147, 2160, 2408
AcoI YGGCCR 2 cut(s) 1549, 2122
AcsI RAATTY 4 cut(s) 208, 231, 1649, 1950
AcuI CTGAAG 3 cut(s) 161, 181, 1614
AfaI GTAC 5 cut(s) 677, 1247, 1383, 2048, 2289
AfeI AGCGCT 1 cut(s) 1826
AfiI CCNNNNNNNGG 2 cut(s) 123, 617
AflII CTTAAG 1 cut(s) 631
AflIII ACRYGT 2 cut(s) 2034, 2176
AgeI ACCGGT 1 cut(s) 219
AhlI ACTAGT 1 cut(s) 343
AjnI CCWGG 6 cut(s) 107, 471, 535, 610, 709, 952
AluBI AGCT 9 cut(s) 71, 216, 442, 1298, 1456, 1585, 1770, 2188, 2474
AluI AGCT 9 cut(s) 71, 216, 442, 1298, 1456, 1585, 1770, 2188, 2474
Alw21I GWGCWC 3 cut(s) 321, 1184, 2190
Alw26I GTCTC 2 cut(s) 1044, 1465
AlwI GGATC 7 cut(s) 701, 1649, 1898, 2105, 2147, 2160, 2408
Aor51HI AGCGCT 1 cut(s) 1826
AoxI GGCC 4 cut(s) 832, 1196, 1549, 2122
ApeKI GCWGC 2 cut(s) 1298, 1514
ApoI RAATTY 4 cut(s) 208, 231, 1649, 1950
AsiGI ACCGGT 1 cut(s) 219
Asp700I GAANNNNTTC 3 cut(s) 1159, 2065, 2395
AspLEI GCGC 3 cut(s) 1413, 1827, 2278
AspS9I GGNCC 2 cut(s) 1196, 1803
AsuC2I CCSGG 2 cut(s) 204, 871
AsuHPI GGTGA 3 cut(s) 1862, 2031, 2357
AsuNHI GCTAGC 1 cut(s) 1372
AvaII GGWCC 1 cut(s) 1803
BaeI ACNNNNGTAYC 2 cut(s) 237, 270
BalI TGGCCA 1 cut(s) 1551
BamHI GGATCC 1 cut(s) 2152
BanI GGYRCC 1 cut(s) 896
BanII GRGCYC 1 cut(s) 2190
BauI CACGAG 2 cut(s) 1533, 1851
Bbv12I GWGCWC 3 cut(s) 321, 1184, 2190
BbvI GCAGC 2 cut(s) 1285, 1526
BccI CCATC 5 cut(s) 143, 305, 821, 1040, 1225
BciT130I CCWGG 6 cut(s) 109, 473, 537, 612, 711, 954
BclI TGATCA 1 cut(s) 511
BcnI CCSGG 2 cut(s) 204, 871
BcoDI GTCTC 2 cut(s) 1044, 1465
BcuI ACTAGT 1 cut(s) 343
BfaI CTAG 9 cut(s) 213, 249, 344, 461, 1373, 1748, 1914, 1928, 1971
BfoI RGCGCY 1 cut(s) 1828
BfrI CTTAAG 1 cut(s) 631
BfuAI ACCTGC 1 cut(s) 2320
BglII AGATCT 1 cut(s) 1264
BisI GCNGC 2 cut(s) 1299, 1515
BlsI GCNGC 2 cut(s) 1300, 1516
Bme1390I CCNGG 8 cut(s) 109, 204, 473, 537, 612, 711, 871, 954
Bme18I GGWCC 1 cut(s) 1803
BmgT120I GGNCC 2 cut(s) 1196, 1803
BmiI GGNNCC 3 cut(s) 128, 898, 2154
BmrFI CCNGG 8 cut(s) 109, 204, 473, 537, 612, 711, 871, 954
BmrI ACTGGG 2 cut(s) 576, 627
BmsI GCATC 4 cut(s) 665, 1921, 2252, 2380
BmtI GCTAGC 1 cut(s) 1376
BmuI ACTGGG 2 cut(s) 576, 627
Bpu10I CCTNAGC 1 cut(s) 2367
BpuEI CTTGAG 1 cut(s) 1864
BpuMI CCSGG 2 cut(s) 204, 871
Bsa29I ATCGAT 1 cut(s) 1094
BsaBI GATNNNNATC 2 cut(s) 984, 2412
BsaJI CCNNGG 7 cut(s) 108, 202, 610, 710, 953, 1552, 2466
BsaWI WCCGGW 1 cut(s) 219
BsaXI ACNNNNNCTCC 4 cut(s) 147, 177, 330, 360
Bsc4I CCNNNNNNNGG 2 cut(s) 123, 617
Bse118I RCCGGY 1 cut(s) 219
Bse1I ACTGG 3 cut(s) 571, 622, 884
Bse3DI GCAATG 1 cut(s) 2360
Bse8I GATNNNNATC 2 cut(s) 984, 2412
BseBI CCWGG 6 cut(s) 109, 473, 537, 612, 711, 954
BseCI ATCGAT 1 cut(s) 1094
BseDI CCNNGG 7 cut(s) 108, 202, 610, 710, 953, 1552, 2466
BseJI GATNNNNATC 2 cut(s) 984, 2412
BseLI CCNNNNNNNGG 2 cut(s) 123, 617
BseMI GCAATG 1 cut(s) 2360
BseMII CTCAG 2 cut(s) 257, 1926
BseNI ACTGG 3 cut(s) 571, 622, 884
BseRI GAGGAG 3 cut(s) 77, 1466, 2346
BseXI GCAGC 2 cut(s) 1285, 1526
BsgI GTGCAG 2 cut(s) 420, 843
Bsh1236I CGCG 1 cut(s) 400
Bsh1285I CGRYCG 2 cut(s) 404, 1290
BshFI GGCC 4 cut(s) 834, 1198, 1551, 2124
BshNI GGYRCC 1 cut(s) 896
BshTI ACCGGT 1 cut(s) 219
BshVI ATCGAT 1 cut(s) 1094
BsiEI CGRYCG 2 cut(s) 404, 1290
BsiHKAI GWGCWC 3 cut(s) 321, 1184, 2190
BsiSI CCGG 4 cut(s) 204, 220, 871, 2125
BslFI GGGAC 4 cut(s) 125, 635, 853, 1606
BslI CCNNNNNNNGG 2 cut(s) 123, 617
BsmAI GTCTC 2 cut(s) 1044, 1465
BsmBI CGTCTC 1 cut(s) 1044
BsmFI GGGAC 4 cut(s) 125, 635, 853, 1606
BsmI GAATGC 1 cut(s) 1288
BsnI GGCC 4 cut(s) 834, 1198, 1551, 2124
Bsp1286I GDGCHC 3 cut(s) 321, 1184, 2190
Bsp1407I TGTACA 3 cut(s) 675, 1245, 2287
Bsp19I CCATGG 2 cut(s) 1552, 2466
BspACI CCGC 5 cut(s) 400, 660, 803, 894, 1170
BspANI GGCC 4 cut(s) 834, 1198, 1551, 2124
BspCNI CTCAG 2 cut(s) 256, 1927
BspDI ATCGAT 1 cut(s) 1094
BspFNI CGCG 1 cut(s) 400
BspHI TCATGA 1 cut(s) 2256
BspLI GGNNCC 3 cut(s) 128, 898, 2154
BspMI ACCTGC 1 cut(s) 2320
BspOI GCTAGC 1 cut(s) 1376
BspPI GGATC 7 cut(s) 701, 1649, 1898, 2105, 2147, 2160, 2408
BspT107I GGYRCC 1 cut(s) 896
BspTI CTTAAG 1 cut(s) 631
BsrDI GCAATG 1 cut(s) 2360
BsrFI RCCGGY 1 cut(s) 219
BsrGI TGTACA 3 cut(s) 675, 1245, 2287
BsrI ACTGG 3 cut(s) 571, 622, 884
BssAI RCCGGY 1 cut(s) 219
BssECI CCNNGG 7 cut(s) 108, 202, 610, 710, 953, 1552, 2466
BssSI CACGAG 2 cut(s) 1533, 1851
BssT1I CCWWGG 2 cut(s) 1552, 2466
Bst2BI CACGAG 2 cut(s) 1533, 1851
Bst2UI CCWGG 6 cut(s) 109, 473, 537, 612, 711, 954
Bst4CI ACNGT 5 cut(s) 24, 790, 1291, 1362, 1803
Bst6I CTCTTC 3 cut(s) 86, 95, 2386
BstAFI CTTAAG 1 cut(s) 631
BstAUI TGTACA 3 cut(s) 675, 1245, 2287
BstC8I GCNNGC 3 cut(s) 1347, 1374, 1398
BstDEI CTNAG 5 cut(s) 243, 1151, 1935, 2209, 2367
BstDSI CCRYGG 2 cut(s) 1552, 2466
BstFNI CGCG 1 cut(s) 400
BstH2I RGCGCY 1 cut(s) 1828
BstHHI GCGC 3 cut(s) 1413, 1827, 2278
BstMAI GTCTC 2 cut(s) 1044, 1465
BstMCI CGRYCG 2 cut(s) 404, 1290
BstMWI GCNNNNNNNGC 3 cut(s) 1304, 1373, 1673
BstNI CCWGG 6 cut(s) 109, 473, 537, 612, 711, 954
BstNSI RCATGY 4 cut(s) 1404, 1759, 2038, 2180
BstSCI CCNGG 8 cut(s) 107, 202, 471, 535, 610, 709, 869, 952
BstUI CGCG 1 cut(s) 400
BstV1I GCAGC 2 cut(s) 1285, 1526
BstX2I RGATCY 2 cut(s) 1264, 2152
BstYI RGATCY 2 cut(s) 1264, 2152
Bsu15I ATCGAT 1 cut(s) 1094
BsuRI GGCC 4 cut(s) 834, 1198, 1551, 2124
BsuTUI ATCGAT 1 cut(s) 1094
BtgI CCRYGG 2 cut(s) 1552, 2466
BtsIMutI CAGTG 3 cut(s) 20, 795, 1182
BveI ACCTGC 1 cut(s) 2320
Cac8I GCNNGC 3 cut(s) 1347, 1374, 1398
CciI TCATGA 1 cut(s) 2256
CfoI GCGC 3 cut(s) 1413, 1827, 2278
Cfr10I RCCGGY 1 cut(s) 219
Cfr13I GGNCC 2 cut(s) 1196, 1803
ClaI ATCGAT 1 cut(s) 1094
Csp6I GTAC 5 cut(s) 676, 1246, 1382, 2047, 2288
CspAI ACCGGT 1 cut(s) 219
CviQI GTAC 5 cut(s) 676, 1246, 1382, 2047, 2288
DdeI CTNAG 5 cut(s) 243, 1151, 1935, 2209, 2367
DrdI GACNNNNNNGTC 1 cut(s) 1508
DseDI GACNNNNNNGTC 1 cut(s) 1508
EaeI YGGCCR 2 cut(s) 1549, 2122
Eam1104I CTCTTC 3 cut(s) 86, 95, 2386
EarI CTCTTC 3 cut(s) 86, 95, 2386
EciI GGCGGA 1 cut(s) 649
Ecl136II GAGCTC 1 cut(s) 2188
Eco130I CCWWGG 2 cut(s) 1552, 2466
Eco24I GRGCYC 1 cut(s) 2190
Eco47I GGWCC 1 cut(s) 1803
Eco47III AGCGCT 1 cut(s) 1826
Eco53kI GAGCTC 1 cut(s) 2188
Eco57I CTGAAG 3 cut(s) 161, 181, 1614
EcoICRI GAGCTC 1 cut(s) 2188
EcoO109I RGGNCCY 1 cut(s) 1196
EcoRI GAATTC 1 cut(s) 1649
EcoRII CCWGG 6 cut(s) 107, 471, 535, 610, 709, 952
EcoT14I CCWWGG 2 cut(s) 1552, 2466
EcoT22I ATGCAT 1 cut(s) 2267
EcoT38I GRGCYC 1 cut(s) 2190
ErhI CCWWGG 2 cut(s) 1552, 2466
Esp3I CGTCTC 1 cut(s) 1044
FalI AAGNNNNNCTT 6 cut(s) 60, 92, 836, 868, 1653, 1685
FaqI GGGAC 4 cut(s) 125, 635, 853, 1606
FauNDI CATATG 1 cut(s) 2026
FbaI TGATCA 1 cut(s) 511
FblI GTMKAC 1 cut(s) 386
Fnu4HI GCNGC 2 cut(s) 1299, 1515
FriOI GRGCYC 1 cut(s) 2190
Fsp4HI GCNGC 2 cut(s) 1299, 1515
FspBI CTAG 9 cut(s) 213, 249, 344, 461, 1373, 1748, 1914, 1928, 1971
GlaI GCGC 3 cut(s) 1412, 1826, 2277
GluI GCNGC 2 cut(s) 1299, 1515
HaeII RGCGCY 1 cut(s) 1828
HaeIII GGCC 4 cut(s) 834, 1198, 1551, 2124
HapII CCGG 4 cut(s) 204, 220, 871, 2125
HhaI GCGC 3 cut(s) 1413, 1827, 2278
Hin6I GCGC 3 cut(s) 1411, 1825, 2276
HinP1I GCGC 3 cut(s) 1411, 1825, 2276
HincII GTYRAC 2 cut(s) 418, 2322
HindII GTYRAC 2 cut(s) 418, 2322
HindIII AAGCTT 1 cut(s) 1768
HpaI GTTAAC 1 cut(s) 418
HpaII CCGG 4 cut(s) 204, 220, 871, 2125
HphI GGTGA 3 cut(s) 1862, 2031, 2357
Hpy166II GTNNAC 7 cut(s) 387, 418, 950, 1248, 2288, 2322, 2386
Hpy188I TCNGA 8 cut(s) 11, 95, 246, 651, 1318, 1957, 2080, 2143
Hpy188III TCNNGA 7 cut(s) 737, 1085, 1499, 1632, 1881, 1928, 2257
Hpy8I GTNNAC 7 cut(s) 387, 418, 950, 1248, 2288, 2322, 2386
HpyAV CCTTC 9 cut(s) 136, 803, 838, 845, 1057, 1187, 1313, 1816, 2166
HpyCH4III ACNGT 5 cut(s) 24, 790, 1291, 1362, 1803
HpyCH4IV ACGT 2 cut(s) 428, 1272
HpyF10VI GCNNNNNNNGC 3 cut(s) 1304, 1373, 1673
HpyF3I CTNAG 5 cut(s) 243, 1151, 1935, 2209, 2367
HpySE526I ACGT 2 cut(s) 428, 1272
HspAI GCGC 3 cut(s) 1411, 1825, 2276
Ksp22I TGATCA 1 cut(s) 511
KspAI GTTAAC 1 cut(s) 418
LmnI GCTCC 3 cut(s) 1100, 1453, 2185
Lsp1109I GCAGC 2 cut(s) 1285, 1526
LweI GCATC 4 cut(s) 665, 1921, 2252, 2380
MaeI CTAG 9 cut(s) 213, 249, 344, 461, 1373, 1748, 1914, 1928, 1971
MaeII ACGT 2 cut(s) 428, 1272
MaeIII GTNAC 4 cut(s) 597, 727, 875, 2037
MflI RGATCY 2 cut(s) 1264, 2152
MhlI GDGCHC 3 cut(s) 321, 1184, 2190
MlsI TGGCCA 1 cut(s) 1551
MluNI TGGCCA 1 cut(s) 1551
MlyI GAGTC 1 cut(s) 1871
MmeI TCCRAC 3 cut(s) 208, 1999, 2088
Mox20I TGGCCA 1 cut(s) 1551
Mph1103I ATGCAT 1 cut(s) 2267
MroXI GAANNNNTTC 3 cut(s) 1159, 2065, 2395
MscI TGGCCA 1 cut(s) 1551
MseI TTAA 6 cut(s) 417, 530, 584, 632, 1077, 1239
MslI CAYNNNNRTG 3 cut(s) 993, 1241, 1405
Msp20I TGGCCA 1 cut(s) 1551
MspCI CTTAAG 1 cut(s) 631
MspI CCGG 4 cut(s) 204, 220, 871, 2125
MspR9I CCNGG 8 cut(s) 109, 204, 473, 537, 612, 711, 871, 954
Mva1269I GAATGC 1 cut(s) 1288
MvaI CCWGG 6 cut(s) 109, 473, 537, 612, 711, 954
MvnI CGCG 1 cut(s) 400
MwoI GCNNNNNNNGC 3 cut(s) 1304, 1373, 1673
NciI CCSGG 2 cut(s) 204, 871
NcoI CCATGG 2 cut(s) 1552, 2466
NdeI CATATG 1 cut(s) 2026
NheI GCTAGC 1 cut(s) 1372
NlaIV GGNNCC 3 cut(s) 128, 898, 2154
NmuCI GTSAC 4 cut(s) 597, 727, 875, 2037
NsiI ATGCAT 1 cut(s) 2267
NspI RCATGY 4 cut(s) 1404, 1759, 2038, 2180
PagI TCATGA 1 cut(s) 2256
PciI ACATGT 2 cut(s) 2034, 2176
PctI GAATGC 1 cut(s) 1288
PdmI GAANNNNTTC 3 cut(s) 1159, 2065, 2395
PflMI CCANNNNNTGG 1 cut(s) 123
PfoI TCCNGGA 1 cut(s) 471
PinAI ACCGGT 1 cut(s) 219
PkrI GCNGC 2 cut(s) 1300, 1516
PleI GAGTC 1 cut(s) 1871
PpsI GAGTC 1 cut(s) 1871
PscI ACATGT 2 cut(s) 2034, 2176
PsiI TTATAA 2 cut(s) 360, 1575
Psp124BI GAGCTC 1 cut(s) 2190
Psp6I CCWGG 6 cut(s) 107, 471, 535, 610, 709, 952
PspGI CCWGG 6 cut(s) 107, 471, 535, 610, 709, 952
PspN4I GGNNCC 3 cut(s) 128, 898, 2154
PspPI GGNCC 2 cut(s) 1196, 1803
PsuI RGATCY 2 cut(s) 1264, 2152
RsaI GTAC 5 cut(s) 677, 1247, 1383, 2048, 2289
RsaNI GTAC 5 cut(s) 676, 1246, 1382, 2047, 2288
RseI CAYNNNNRTG 3 cut(s) 993, 1241, 1405
SacI GAGCTC 1 cut(s) 2190
SaqAI TTAA 6 cut(s) 417, 530, 584, 632, 1077, 1239
SatI GCNGC 2 cut(s) 1299, 1515
Sau96I GGNCC 2 cut(s) 1196, 1803
SchI GAGTC 1 cut(s) 1871
ScrFI CCNGG 8 cut(s) 109, 204, 473, 537, 612, 711, 871, 954
SduI GDGCHC 3 cut(s) 321, 1184, 2190
SfaNI GCATC 4 cut(s) 665, 1921, 2252, 2380
SinI GGWCC 1 cut(s) 1803
SmiMI CAYNNNNRTG 3 cut(s) 993, 1241, 1405
SmlI CTYRAG 2 cut(s) 631, 1879
SmoI CTYRAG 2 cut(s) 631, 1879
SpeI ACTAGT 1 cut(s) 343
SsiI CCGC 5 cut(s) 400, 660, 803, 894, 1170
SspI AATATT 2 cut(s) 1921, 1977
SspMI CTAG 9 cut(s) 213, 249, 344, 461, 1373, 1748, 1914, 1928, 1971
SstI GAGCTC 1 cut(s) 2190
StyD4I CCNGG 8 cut(s) 107, 202, 471, 535, 610, 709, 869, 952
StyI CCWWGG 2 cut(s) 1552, 2466
TaaI ACNGT 5 cut(s) 24, 790, 1291, 1362, 1803
TaiI ACGT 2 cut(s) 431, 1275
TaqI TCGA 6 cut(s) 686, 1021, 1094, 1494, 1644, 2476
TaqII GACCGA 1 cut(s) 2322
TatI WGTACW 3 cut(s) 675, 1245, 2287
Tru1I TTAA 6 cut(s) 417, 530, 584, 632, 1077, 1239
Tru9I TTAA 6 cut(s) 417, 530, 584, 632, 1077, 1239
TscAI CASTG 3 cut(s) 27, 795, 1189
TseFI GTSAC 4 cut(s) 597, 727, 875, 2037
TseI GCWGC 2 cut(s) 1298, 1514
Tsp45I GTSAC 4 cut(s) 597, 727, 875, 2037
TspGWI ACGGA 1 cut(s) 1265
TspRI CASTG 3 cut(s) 27, 795, 1189
Van91I CCANNNNNTGG 1 cut(s) 123
Vha464I CTTAAG 1 cut(s) 631
VpaK11BI GGWCC 1 cut(s) 1803
XapI RAATTY 4 cut(s) 208, 231, 1649, 1950
XbaI TCTAGA 1 cut(s) 1927
XceI RCATGY 4 cut(s) 1404, 1759, 2038, 2180
XcmI CCANNNNNNNNNTGG 1 cut(s) 1559
XmiI GTMKAC 1 cut(s) 386
XmnI GAANNNNTTC 3 cut(s) 1159, 2065, 2395
XspI CTAG 9 cut(s) 213, 249, 344, 461, 1373, 1748, 1914, 1928, 1971
Zsp2I ATGCAT 1 cut(s) 2267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.