Rroxscaffold_1G00070550

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000112
Physical Location & Seq
Reverse (-)
91456414 .. 91473746
17333 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00070550.1

Sequence Viewer

Length: 2427 bp
ATGGTGGAGGCGGATGAACTTGAGGAAACTAAGAGACACCCTGAATTGCAATTTTTCAATTTGAACACACTAATGGCAGCCACAGATAACTTCTCTCATGTCAATGAACTCGGTCAAGGTGGTTTTGGCTCTGTTTATAAGGGTCAGTTACCAAATGAGCAGAAGGTTGCTGTAAAAAGGTTGTCTAAAACATCAGGACAAGGGACTGAAGAATTCAAAAATGAAGTTGCACTTATAGCAAGACTTCAACACCGCGCAATCTCTGGCTATATGTCACCAGAGTATGCAGTATTTGGCAGATTTTCTATTAAATCCGATGTCTTTAGTTTTGGGATCATTTTGTTAGAGGTTGTAACTGGCAAAAGAAACAATGGTTTTTCTCAAGAGGATCCTTCCATGAACTTGATAAGACATGTTTGGGAACTCTGGAGAGAAGACAGAGCCTTAGAACTTGTGGATTCGAATCTGGAGTCATATAATTCTGATGAGGTCATGAGATGCATTCAAGTTGCGCTCTTGTGCGTGCAAGAAGAATCAAGGGACAGGCCAGCCATGTCTACCATTGTTTTCATGTTGAGTGGTGAAACATCTCCTCCACTGCCTAACCAACCTGCATATGTTTGCAGAAGAACCTCCAGCACTGATGCTGATCCATCAGTACCAAAACGATTATATTCTATAAACGACTTGACAATAACTGCAGAGGAAGGTCGGGACACCATAACATGGAACAAGGAACAACTGAAGGATGATGGAGGAGTTTTAGTGTCCAAACAAAGCAAGTTCGAATTGGGTTTCTTCAGCCCCGGAAATTCTAGCTACCGGTATGTTGGAATTTGGTACTCTTTGGCTAAAGTATCTGGAAAAACAGTGGTGTGGGTTGCAAACAGGAACAATCCCATCAATGATACCTCTGGTGTGTTCACAATAAACAGGTATGGAGAACTAGTCCTTCATGCTTATAACATGGAGAGCATTCCTATTTGGTCTACTAATGTGTTGCGGTCGGTTCAAAATGTTAACACAAGCACTTCATCTGCACAGCTTTTAGATTCAGGAAATTTAGTTGTGTTCCAGGACGATAAAAATGAAATCTTTATATGGCAAAGTTTTGATCATCCTACAGATACTTTAATTCCAGGAATGAAAGTTGGGGTGAATTGGAAAACTGGGCTAGAATGGGTTTTAACATCTTGGAAGTCACAAGATGACCCTGGAACTGGGGACTATACCTTAAGGCTCTATCCAAATCAGATTGCATCCCCCCAATTTTTTATGTACAAGGGTCTGAGTATGTATTGGCGAAGTGATCCAGGGCCAACGCCTAGTGCGGTCACTAATCAAGAGGAAACTTATTATCTCATGGATGACACCAATGCAATTACAAGAGTAACACTGTCTGATTCTGTGGACCAGCGCCTTACATGGGATGATGGTGACCTTCAATGGAAGGAAGGCTTCTCTGCACCGAAGTCCCAGTGTGATTGGTATGGACAGTGTGGTGCCAACAGCAAATGTGGCCCTGACAATGTTAATATGTTTTGGTGTGATTGTTTATTACCAGGGAATGTGCCTAATTCTATAAGTGATTGGAATCAGAAAAATGGTTCTGGTGGATGTGTGAGTAATCGACCTGGTTTGTTGAAGTGTGGAGATGGAGATGGATTTATAAAGGTGGCAAGAGTTAAATATCCAGACACATCAATAGCAGCATACACTGAAAATGAAGGGATTGTTGATTGCTTGACATGGTATGATGACTTGATGGACATTTTAGTGTACACAAAGCTTGGACAAGATCTCTACGTTCGTGTGAATGCAACCGTGTTAGGACAAGGGATTGAAGAATTCAAAAATGAAGTAGCACTTATAGCAAGACTTCAACACAGGAATCTTGTGAAACTTTTAGATACTGAGATGAACCCAAAAATTTCTGATTTTGGCATGGCTAGAATATTCCACGGAGACCAACTGCAAGATAAGACAAGCCGAATTGTCAGAACATATGGCTACATGTCGCCAGAGTATGCAGTGTTTGGGAGATTTTCCATGAAATCTGATATCTTTAGTTTTGGGATTATAATTTTGGAGATTATAAGTAGCCAGAAAAACAATGGTTCATATGTTTGGGAGCTCTGGGGAGAAGGCAGAGCCTTAGATATTTTGGATTCGACACTGAAGTCATATCAGCCTAATGAAGTCATGAGATACATACAAGTTGCGCTCTTGTGTGTACAAGAAGATTCAAAGGACCGACCTGCCATGTCAGCCATTGTTTTCATGTTGAGTGGTGAAGCATCTCCTCCATTACCAAAGCAGCCAGCATTTGTTTACAGAAGAGATTCCCGCGCTGATGTTGATCCATTACTTCCGAAAGGATCTTATTCTATAAACGACTTGACAATAACTACAATGGAAGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

808

Amino Acids

90.95

Weight (kDa)

4.97

Isoelectric Point (pI)

35.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 32 - 87 9.7e-06 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 34 - 87 1.1e-06 Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr PF07714 90 - 190 1e-07 Protein tyrosine and serine/threonine kinase
B_lectin PF01453 290 - 400 8.8e-36 D-mannose binding lectin
S_locus_glycop PF00954 449 - 523 1.8e-10 S-locus glycoprotein domain
PK_Tyr_Ser-Thr PF07714 635 - 760 1.5e-15 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 635 - 703 1.4e-09 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 5 cut(s) 138, 963, 1670, 2081, 2096
AasI GACNNNNNNGTC 1 cut(s) 2179
Acc36I ACCTGC 2 cut(s) 619, 2266
AccB1I GGYRCC 1 cut(s) 1502
AccB7I CCANNNNNTGG 1 cut(s) 726
AccI GTMKAC 2 cut(s) 557, 989
AccII CGCG 2 cut(s) 255, 2349
AciI CCGC 5 cut(s) 11, 253, 1003, 1331, 2347
AclWI GGATC 7 cut(s) 341, 383, 396, 644, 1304, 2354, 2386
AcsI RAATTY 6 cut(s) 212, 811, 834, 1060, 1847, 1929
AcuI CTGAAG 4 cut(s) 228, 764, 784, 2198
AfaI GTAC 5 cut(s) 660, 842, 1280, 1781, 2235
AfiI CCNNNNNNNGG 3 cut(s) 726, 1220, 1426
AflII CTTAAG 1 cut(s) 1234
AflIII ACRYGT 2 cut(s) 412, 2013
AgeI ACCGGT 1 cut(s) 822
AhlI ACTAGT 1 cut(s) 946
AjnI CCWGG 6 cut(s) 1074, 1138, 1213, 1312, 1561, 1633
AluBI AGCT 5 cut(s) 819, 1045, 1789, 2134, 2420
AluI AGCT 5 cut(s) 819, 1045, 1789, 2134, 2420
Alw21I GWGCWC 1 cut(s) 2136
Alw26I GTCTC 2 cut(s) 28, 1959
AlwI GGATC 7 cut(s) 341, 383, 396, 644, 1304, 2354, 2386
AoxI GGCC 3 cut(s) 545, 1316, 1519
ApeKI GCWGC 3 cut(s) 77, 1709, 2317
ApoI RAATTY 6 cut(s) 212, 811, 834, 1060, 1847, 1929
ArsI GACNNNNNNTTYG 2 cut(s) 455, 487
AsiGI ACCGGT 1 cut(s) 822
Asp700I GAANNNNTTC 1 cut(s) 2341
AspLEI GCGC 5 cut(s) 257, 514, 1419, 2224, 2351
AspS9I GGNCC 4 cut(s) 1316, 1411, 1520, 2251
AsuC2I CCSGG 1 cut(s) 807
AsuHPI GGTGA 5 cut(s) 267, 593, 1168, 1448, 2303
AsuII TTCGAA 2 cut(s) 461, 786
AvaII GGWCC 2 cut(s) 1411, 2251
BamHI GGATCC 1 cut(s) 388
BanI GGYRCC 1 cut(s) 1502
BanII GRGCYC 1 cut(s) 2136
BbsI GAAGAC 1 cut(s) 441
Bbv12I GWGCWC 1 cut(s) 2136
BbvI GCAGC 3 cut(s) 89, 1721, 2329
BccI CCATC 7 cut(s) 661, 746, 908, 1427, 1649, 1655, 1759
BciT130I CCWGG 6 cut(s) 1076, 1140, 1215, 1314, 1563, 1635
BclI TGATCA 1 cut(s) 1114
BcnI CCSGG 1 cut(s) 807
BcoDI GTCTC 2 cut(s) 28, 1959
BcuI ACTAGT 1 cut(s) 946
BfaI CTAG 5 cut(s) 816, 947, 1175, 1326, 1950
BfmI CTRYAG 2 cut(s) 699, 1122
BfoI RGCGCY 1 cut(s) 1420
BfrI CTTAAG 1 cut(s) 1234
BfuAI ACCTGC 2 cut(s) 619, 2266
BglII AGATCT 1 cut(s) 1798
BisI GCNGC 3 cut(s) 78, 1710, 2318
BlsI GCNGC 3 cut(s) 79, 1711, 2319
Bme1390I CCNGG 7 cut(s) 807, 1076, 1140, 1215, 1314, 1563, 1635
Bme18I GGWCC 2 cut(s) 1411, 2251
BmgT120I GGNCC 4 cut(s) 1316, 1411, 1520, 2251
BmiI GGNNCC 2 cut(s) 390, 1504
BmrFI CCNGG 7 cut(s) 807, 1076, 1140, 1215, 1314, 1563, 1635
BmrI ACTGGG 3 cut(s) 1179, 1230, 1471
BmsI GCATC 4 cut(s) 488, 634, 1268, 2306
BmuI ACTGGG 3 cut(s) 1179, 1230, 1471
BpiI GAAGAC 1 cut(s) 441
BpmI CTGGAG 3 cut(s) 448, 488, 619
Bpu14I TTCGAA 2 cut(s) 461, 786
BpuEI CTTGAG 2 cut(s) 41, 366
BpuMI CCSGG 1 cut(s) 807
BsaBI GATNNNNATC 4 cut(s) 462, 648, 1593, 2358
BsaI GGTCTC 1 cut(s) 1959
BsaJI CCNNGG 5 cut(s) 805, 1213, 1313, 1562, 1960
BsaWI WCCGGW 1 cut(s) 822
BsaXI ACNNNNNCTCC 6 cut(s) 577, 607, 750, 780, 933, 963
Bsc4I CCNNNNNNNGG 3 cut(s) 726, 1220, 1426
Bse118I RCCGGY 1 cut(s) 822
Bse1I ACTGG 4 cut(s) 361, 1174, 1225, 1477
Bse8I GATNNNNATC 4 cut(s) 462, 648, 1593, 2358
BseBI CCWGG 6 cut(s) 1076, 1140, 1215, 1314, 1563, 1635
BseDI CCNNGG 5 cut(s) 805, 1213, 1313, 1562, 1960
BseGI GGATG 7 cut(s) 19, 754, 1117, 1259, 1372, 1435, 1622
BseJI GATNNNNATC 4 cut(s) 462, 648, 1593, 2358
BseLI CCNNNNNNNGG 3 cut(s) 726, 1220, 1426
BseMII CTCAG 2 cut(s) 1280, 1905
BseNI ACTGG 4 cut(s) 361, 1174, 1225, 1477
BseRI GAGGAG 3 cut(s) 582, 771, 2292
BseXI GCAGC 3 cut(s) 89, 1721, 2329
BsgI GTGCAG 2 cut(s) 1023, 1449
Bsh1236I CGCG 2 cut(s) 255, 2349
Bsh1285I CGRYCG 1 cut(s) 1007
BshFI GGCC 3 cut(s) 547, 1318, 1521
BshNI GGYRCC 1 cut(s) 1502
BshTI ACCGGT 1 cut(s) 822
BsiEI CGRYCG 1 cut(s) 1007
BsiHKAI GWGCWC 1 cut(s) 2136
BsiSI CCGG 2 cut(s) 807, 823
BslFI GGGAC 5 cut(s) 217, 554, 728, 1238, 1459
BslI CCNNNNNNNGG 3 cut(s) 726, 1220, 1426
BsmAI GTCTC 2 cut(s) 28, 1959
BsmFI GGGAC 5 cut(s) 217, 554, 728, 1238, 1459
BsmI GAATGC 3 cut(s) 501, 975, 1822
BsnI GGCC 3 cut(s) 547, 1318, 1521
Bso31I GGTCTC 1 cut(s) 1959
Bsp119I TTCGAA 2 cut(s) 461, 786
Bsp1286I GDGCHC 1 cut(s) 2136
Bsp1407I TGTACA 3 cut(s) 1278, 1779, 2233
Bsp143I GATC 8 cut(s) 333, 388, 649, 1114, 1309, 1798, 2359, 2378
BspACI CCGC 5 cut(s) 11, 253, 1003, 1331, 2347
BspANI GGCC 3 cut(s) 547, 1318, 1521
BspCNI CTCAG 2 cut(s) 1281, 1906
BspFNI CGCG 2 cut(s) 255, 2349
BspHI TCATGA 2 cut(s) 492, 2202
BspLI GGNNCC 2 cut(s) 390, 1504
BspMAI CTGCAG 1 cut(s) 703
BspMI ACCTGC 2 cut(s) 619, 2266
BspPI GGATC 7 cut(s) 341, 383, 396, 644, 1304, 2354, 2386
BspT104I TTCGAA 2 cut(s) 461, 786
BspT107I GGYRCC 1 cut(s) 1502
BspTI CTTAAG 1 cut(s) 1234
BspTNI GGTCTC 1 cut(s) 1959
BsrFI RCCGGY 1 cut(s) 822
BsrGI TGTACA 3 cut(s) 1278, 1779, 2233
BsrI ACTGG 4 cut(s) 361, 1174, 1225, 1477
BssAI RCCGGY 1 cut(s) 822
BssECI CCNNGG 5 cut(s) 805, 1213, 1313, 1562, 1960
BssMI GATC 8 cut(s) 333, 388, 649, 1114, 1309, 1798, 2359, 2378
Bst2UI CCWGG 6 cut(s) 1076, 1140, 1215, 1314, 1563, 1635
Bst4CI ACNGT 4 cut(s) 871, 1398, 1497, 1825
Bst6I CTCTTC 1 cut(s) 2332
BstAFI CTTAAG 1 cut(s) 1234
BstAUI TGTACA 3 cut(s) 1278, 1779, 2233
BstBI TTCGAA 2 cut(s) 461, 786
BstC8I GCNNGC 3 cut(s) 524, 549, 2322
BstDEI CTNAG 5 cut(s) 30, 445, 1289, 1914, 2155
BstDSI CCRYGG 1 cut(s) 1960
BstEII GGTNACC 1 cut(s) 1436
BstF5I GGATG 7 cut(s) 19, 754, 1117, 1259, 1372, 1435, 1622
BstFNI CGCG 2 cut(s) 255, 2349
BstH2I RGCGCY 1 cut(s) 1420
BstHHI GCGC 5 cut(s) 257, 514, 1419, 2224, 2351
BstKTI GATC 8 cut(s) 336, 391, 652, 1117, 1312, 1801, 2362, 2381
BstMAI GTCTC 2 cut(s) 28, 1959
BstMBI GATC 8 cut(s) 333, 388, 649, 1114, 1309, 1798, 2359, 2378
BstMCI CGRYCG 1 cut(s) 1007
BstMWI GCNNNNNNNGC 4 cut(s) 236, 1518, 1871, 2267
BstNI CCWGG 6 cut(s) 1076, 1140, 1215, 1314, 1563, 1635
BstNSI RCATGY 2 cut(s) 416, 2017
BstPI GGTNACC 1 cut(s) 1436
BstSCI CCNGG 7 cut(s) 805, 1074, 1138, 1213, 1312, 1561, 1633
BstSFI CTRYAG 2 cut(s) 699, 1122
BstUI CGCG 2 cut(s) 255, 2349
BstV1I GCAGC 3 cut(s) 89, 1721, 2329
BstV2I GAAGAC 1 cut(s) 441
BstX2I RGATCY 3 cut(s) 388, 1798, 2378
BstYI RGATCY 3 cut(s) 388, 1798, 2378
BsuRI GGCC 3 cut(s) 547, 1318, 1521
BtgI CCRYGG 1 cut(s) 1960
BtsCI GGATG 7 cut(s) 19, 754, 1117, 1259, 1372, 1435, 1622
BtsI GCAGTG 2 cut(s) 596, 2037
BtsIMutI CAGTG 9 cut(s) 596, 639, 876, 1394, 1484, 1502, 1716, 2037, 2174
BveI ACCTGC 2 cut(s) 619, 2266
Cac8I GCNNGC 3 cut(s) 524, 549, 2322
CciI TCATGA 2 cut(s) 492, 2202
CfoI GCGC 5 cut(s) 257, 514, 1419, 2224, 2351
Cfr10I RCCGGY 1 cut(s) 822
Cfr13I GGNCC 4 cut(s) 1316, 1411, 1520, 2251
CsiI ACCWGGT 1 cut(s) 1633
Csp6I GTAC 5 cut(s) 659, 841, 1279, 1780, 2234
CspAI ACCGGT 1 cut(s) 822
CviQI GTAC 5 cut(s) 659, 841, 1279, 1780, 2234
DdeI CTNAG 5 cut(s) 30, 445, 1289, 1914, 2155
DpnI GATC 8 cut(s) 335, 390, 651, 1116, 1311, 1800, 2361, 2380
DpnII GATC 8 cut(s) 333, 388, 649, 1114, 1309, 1798, 2359, 2378
DrdI GACNNNNNNGTC 1 cut(s) 2179
DseDI GACNNNNNNGTC 1 cut(s) 2179
Eam1104I CTCTTC 1 cut(s) 2332
EarI CTCTTC 1 cut(s) 2332
EciI GGCGGA 1 cut(s) 26
Ecl136II GAGCTC 1 cut(s) 2134
Eco24I GRGCYC 1 cut(s) 2136
Eco31I GGTCTC 1 cut(s) 1959
Eco32I GATATC 1 cut(s) 2062
Eco47I GGWCC 2 cut(s) 1411, 2251
Eco53kI GAGCTC 1 cut(s) 2134
Eco57I CTGAAG 4 cut(s) 228, 764, 784, 2198
Eco91I GGTNACC 1 cut(s) 1436
EcoICRI GAGCTC 1 cut(s) 2134
EcoO65I GGTNACC 1 cut(s) 1436
EcoRI GAATTC 2 cut(s) 212, 1847
EcoRII CCWGG 6 cut(s) 1074, 1138, 1213, 1312, 1561, 1633
EcoRV GATATC 1 cut(s) 2062
EcoT22I ATGCAT 1 cut(s) 503
EcoT38I GRGCYC 1 cut(s) 2136
FalI AAGNNNNNCTT 6 cut(s) 216, 248, 1442, 1474, 1851, 1883
FaqI GGGAC 5 cut(s) 217, 554, 728, 1238, 1459
FauI CCCGC 1 cut(s) 2354
FauNDI CATATG 3 cut(s) 616, 2005, 2122
FbaI TGATCA 1 cut(s) 1114
FblI GTMKAC 2 cut(s) 557, 989
Fnu4HI GCNGC 3 cut(s) 78, 1710, 2318
FokI GGATG 7 cut(s) 26, 761, 1104, 1246, 1379, 1442, 1629
FriOI GRGCYC 1 cut(s) 2136
Fsp4HI GCNGC 3 cut(s) 78, 1710, 2318
FspBI CTAG 5 cut(s) 816, 947, 1175, 1326, 1950
GlaI GCGC 5 cut(s) 256, 513, 1418, 2223, 2350
GluI GCNGC 3 cut(s) 78, 1710, 2318
GsuI CTGGAG 3 cut(s) 448, 488, 619
HaeII RGCGCY 1 cut(s) 1420
HaeIII GGCC 3 cut(s) 547, 1318, 1521
HapII CCGG 2 cut(s) 807, 823
HhaI GCGC 5 cut(s) 257, 514, 1419, 2224, 2351
Hin6I GCGC 5 cut(s) 255, 512, 1417, 2222, 2349
HinP1I GCGC 5 cut(s) 255, 512, 1417, 2222, 2349
HincII GTYRAC 1 cut(s) 1021
HindII GTYRAC 1 cut(s) 1021
HindIII AAGCTT 1 cut(s) 1787
HpaI GTTAAC 1 cut(s) 1021
HpaII CCGG 2 cut(s) 807, 823
HphI GGTGA 5 cut(s) 267, 593, 1168, 1448, 2303
Hpy166II GTNNAC 9 cut(s) 558, 924, 990, 1021, 1411, 1780, 1782, 2234, 2332
Hpy8I GTNNAC 9 cut(s) 558, 924, 990, 1021, 1411, 1780, 1782, 2234, 2332
HpyCH4III ACNGT 4 cut(s) 871, 1398, 1497, 1825
HpyCH4IV ACGT 1 cut(s) 1806
HpyF10VI GCNNNNNNNGC 4 cut(s) 236, 1518, 1871, 2267
HpyF3I CTNAG 5 cut(s) 30, 445, 1289, 1914, 2155
HpySE526I ACGT 1 cut(s) 1806
HspAI GCGC 5 cut(s) 255, 512, 1417, 2222, 2349
Ksp22I TGATCA 1 cut(s) 1114
KspAI GTTAAC 1 cut(s) 1021
Kzo9I GATC 8 cut(s) 333, 388, 649, 1114, 1309, 1798, 2359, 2378
LmnI GCTCC 1 cut(s) 2131
Lsp1109I GCAGC 3 cut(s) 89, 1721, 2329
LweI GCATC 4 cut(s) 488, 634, 1268, 2306
MabI ACCWGGT 1 cut(s) 1633
MaeI CTAG 5 cut(s) 816, 947, 1175, 1326, 1950
MaeII ACGT 1 cut(s) 1806
MaeIII GTNAC 7 cut(s) 147, 273, 352, 1200, 1333, 1390, 1436
MalI GATC 8 cut(s) 335, 390, 651, 1116, 1311, 1800, 2361, 2380
MboI GATC 8 cut(s) 333, 388, 649, 1114, 1309, 1798, 2359, 2378
MboII GAAGA 8 cut(s) 221, 446, 542, 639, 790, 1856, 2252, 2349
MflI RGATCY 3 cut(s) 388, 1798, 2378
MhlI GDGCHC 1 cut(s) 2136
MlyI GAGTC 1 cut(s) 479
MmeI TCCRAC 1 cut(s) 811
Mph1103I ATGCAT 1 cut(s) 503
MroXI GAANNNNTTC 1 cut(s) 2341
MseI TTAA 7 cut(s) 309, 1020, 1133, 1187, 1235, 1533, 1686
MslI CAYNNNNRTG 3 cut(s) 71, 102, 1775
MspCI CTTAAG 1 cut(s) 1234
MspI CCGG 2 cut(s) 807, 823
MspR9I CCNGG 7 cut(s) 807, 1076, 1140, 1215, 1314, 1563, 1635
Mva1269I GAATGC 3 cut(s) 501, 975, 1822
MvaI CCWGG 6 cut(s) 1076, 1140, 1215, 1314, 1563, 1635
MvnI CGCG 2 cut(s) 255, 2349
MwoI GCNNNNNNNGC 4 cut(s) 236, 1518, 1871, 2267
NciI CCSGG 1 cut(s) 807
NdeI CATATG 3 cut(s) 616, 2005, 2122
NdeII GATC 8 cut(s) 333, 388, 649, 1114, 1309, 1798, 2359, 2378
NlaIV GGNNCC 2 cut(s) 390, 1504
NmuCI GTSAC 4 cut(s) 273, 1200, 1333, 1436
NsiI ATGCAT 1 cut(s) 503
NspI RCATGY 2 cut(s) 416, 2017
NspV TTCGAA 2 cut(s) 461, 786
PagI TCATGA 2 cut(s) 492, 2202
PciI ACATGT 2 cut(s) 412, 2013
PctI GAATGC 3 cut(s) 501, 975, 1822
PdmI GAANNNNTTC 1 cut(s) 2341
PflMI CCANNNNNTGG 1 cut(s) 726
PfoI TCCNGGA 2 cut(s) 1074, 1138
PinAI ACCGGT 1 cut(s) 822
PkrI GCNGC 3 cut(s) 79, 1711, 2319
PleI GAGTC 1 cut(s) 478
PpsI GAGTC 1 cut(s) 478
PscI ACATGT 2 cut(s) 412, 2013
PsiI TTATAA 5 cut(s) 138, 963, 1670, 2081, 2096
Psp124BI GAGCTC 1 cut(s) 2136
Psp6I CCWGG 6 cut(s) 1074, 1138, 1213, 1312, 1561, 1633
PspEI GGTNACC 1 cut(s) 1436
PspGI CCWGG 6 cut(s) 1074, 1138, 1213, 1312, 1561, 1633
PspN4I GGNNCC 2 cut(s) 390, 1504
PspPI GGNCC 4 cut(s) 1316, 1411, 1520, 2251
PstI CTGCAG 1 cut(s) 703
PsuI RGATCY 3 cut(s) 388, 1798, 2378
RsaI GTAC 5 cut(s) 660, 842, 1280, 1781, 2235
RsaNI GTAC 5 cut(s) 659, 841, 1279, 1780, 2234
RseI CAYNNNNRTG 3 cut(s) 71, 102, 1775
SacI GAGCTC 1 cut(s) 2136
SaqAI TTAA 7 cut(s) 309, 1020, 1133, 1187, 1235, 1533, 1686
SatI GCNGC 3 cut(s) 78, 1710, 2318
Sau3AI GATC 8 cut(s) 333, 388, 649, 1114, 1309, 1798, 2359, 2378
Sau96I GGNCC 4 cut(s) 1316, 1411, 1520, 2251
SchI GAGTC 1 cut(s) 479
ScrFI CCNGG 7 cut(s) 807, 1076, 1140, 1215, 1314, 1563, 1635
SduI GDGCHC 1 cut(s) 2136
SexAI ACCWGGT 1 cut(s) 1633
SfaNI GCATC 4 cut(s) 488, 634, 1268, 2306
SfcI CTRYAG 2 cut(s) 699, 1122
SfuI TTCGAA 2 cut(s) 461, 786
SinI GGWCC 2 cut(s) 1411, 2251
SmiMI CAYNNNNRTG 3 cut(s) 71, 102, 1775
SmlI CTYRAG 3 cut(s) 20, 381, 1234
SmoI CTYRAG 3 cut(s) 20, 381, 1234
SpeI ACTAGT 1 cut(s) 946
SsiI CCGC 5 cut(s) 11, 253, 1003, 1331, 2347
SspI AATATT 1 cut(s) 1956
SspMI CTAG 5 cut(s) 816, 947, 1175, 1326, 1950
SstI GAGCTC 1 cut(s) 2136
StyD4I CCNGG 7 cut(s) 805, 1074, 1138, 1213, 1312, 1561, 1633
TaaI ACNGT 4 cut(s) 871, 1398, 1497, 1825
TaiI ACGT 1 cut(s) 1809
TaqI TCGA 5 cut(s) 461, 786, 1630, 2171, 2422
TaqII GACCGA 2 cut(s) 101, 2268
TatI WGTACW 3 cut(s) 1278, 1779, 2233
Tru1I TTAA 7 cut(s) 309, 1020, 1133, 1187, 1235, 1533, 1686
Tru9I TTAA 7 cut(s) 309, 1020, 1133, 1187, 1235, 1533, 1686
TscAI CASTG 9 cut(s) 603, 646, 876, 1401, 1484, 1502, 1723, 2037, 2181
TseFI GTSAC 4 cut(s) 273, 1200, 1333, 1436
TseI GCWGC 3 cut(s) 77, 1709, 2317
Tsp45I GTSAC 4 cut(s) 273, 1200, 1333, 1436
TspGWI ACGGA 1 cut(s) 1977
TspRI CASTG 9 cut(s) 603, 646, 876, 1401, 1484, 1502, 1723, 2037, 2181
Van91I CCANNNNNTGG 1 cut(s) 726
Vha464I CTTAAG 1 cut(s) 1234
VpaK11BI GGWCC 2 cut(s) 1411, 2251
XapI RAATTY 6 cut(s) 212, 811, 834, 1060, 1847, 1929
XceI RCATGY 2 cut(s) 416, 2017
XcmI CCANNNNNNNNNTGG 1 cut(s) 2111
XmiI GTMKAC 2 cut(s) 557, 989
XmnI GAANNNNTTC 1 cut(s) 2341
XspI CTAG 5 cut(s) 816, 947, 1175, 1326, 1950
Zsp2I ATGCAT 1 cut(s) 503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.