Rh5AG043100

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000112
Physical Location & Seq
Forward (+)
3406829 .. 3409845
3017 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG043100.1

Sequence Viewer

Length: 1551 bp
ATGTTTTTGAATACTTTTCTTCTGTTCCTTCTCTTCCCTTTTTGCATTTCCATAGACACCATAGGACCAAACCAGTATGTCAACGATGGTGAGACCATAGTGTCTAAAGAAATGAAGTTTGAGCTAGGCTTCTTTAGCCCTGGCAACTCTAGTTATCGGTATGTTGGAATTCGGTATGCTAAAATACCTGAAAAACCAGTGATATGGGTGGCAAACAGGGACAATCCGATTAATGATTCATCTGGTGTCCTCACAAACAGGTATGGAAACTTGGTTCTTTATGCTTATAAGGAAAACTTAACTATTTGGTCCACAAAGGTGTCAGACCAAACCACTACTAGTACTTCAGCTCAAATTTTAGATACAGGAAACTTGATTTTGGTCCAGAGTAACAGTGAAGACTTTTTATGGCAAAGCTTTGATTATCCAACGGATACTATGATGCCAGGAATGAAACTTGGGTTGAATCGTGAGACAGGTCTGGATAGCTTCATAACATCCTGGAGGTCAGAAGATGACCCTGGAACTGGAGATTATTCATATAGGCTCAACCTGACTGGCTCCCCACAATTCATTCTGTATAATGGTTCAACTAGGTATTTTCGAACTGCTATGTGGCCATGGAGCCCATCACCGAGTACAACACCTTATGCATATGACTACACTTTTGTCAATAATCAAGATGTGACATATTTGATTGATTCTTCTAATAAATCCTCTGAAATTTCAAGACTTGTGGTTAACTATTATGGCACACTTCAGCACCTAACATGGAGTGATGTTGAGGGTCAATGGAAGGAAAAGTGGGCTGCACCAAAGTTCAGGTGTGACCCATACGGACATTGTGGCGCCTTCAGTGGATGTAGTCTTGACAATGTTCAATCTGAGTGTTCTTGTTTGCCAGGGTATCAACCAAGTTCTCCAGAGAATTGGTCTGAGAAAATTTGGTCAGATGGGTGTGTGAGTAAGCAGGTAGAAGTACCTAATAAGTGTGGAAAAAGTTTCGTGAGGGTGGAACATGTGAAGCTTCCAGATGCATCAATATCAGCTTTGCTGGACACAAAAATGGGTGAAAGTGAGTGCAAGAGGGCATGCCAGAATAATTGTTCATGCGTCGCATATTCAAGTCTAGAAATTGATGGAGGTGGTGGTTGCATGGCATGGTATGGGGAATTGGTGGACACTTTAATACTGGCAGAGCAGGGACGAGATTTCTATGTTCGAGCAGATGCAACTGAGTCAGCTGAGAATGGCAGAAAATCAAGAGGTTTCCTCAGAAGGAGGGGAATGCTTGCTGTTCTAATTATACCTGTTCTGCTGGCACTTTCTCTGATCATGTTGGCCTATTGCTGGTTTATCAAGAGGAAGGGCTATGTTGCAAAGGTGAAACTGGGTTCACATCCAAAACCAATTGGCAATGGATGGAGTAGCCCAAACCCTTATAAACCCATAGGCGGTCCCATTTTTCCTATGTGGCATGTATATATTCTCAACACGCCCCCGCACGTGTGGCGAATTTTCAAGCCATACACGTGGACAACTTTTGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

516

Amino Acids

58.2

Weight (kDa)

6.61

Isoelectric Point (pI)

40.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 67 - 169 2.8e-35 D-mannose binding lectin
S_locus_glycop PF00954 217 - 306 4e-18 S-locus glycoprotein domain
PAN_2 PF08276 332 - 395 2.2e-17 PAN-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 288, 1443
AasI GACNNNNNNGTC 1 cut(s) 100
Acc36I ACCTGC 1 cut(s) 961
AccB1I GGYRCC 1 cut(s) 848
AciI CCGC 2 cut(s) 1455, 1502
AcoI YGGCCR 1 cut(s) 617
AcsI RAATTY 5 cut(s) 168, 354, 723, 942, 1515
AcuI CTGAAG 3 cut(s) 330, 743, 838
AcvI CACGTG 2 cut(s) 1507, 1533
AcyI GRCGYC 1 cut(s) 849
AfaI GTAC 3 cut(s) 343, 640, 981
AfiI CCNNNNNNNGG 3 cut(s) 527, 1350, 1454
AflIII ACRYGT 3 cut(s) 1018, 1506, 1530
AgsI TTSAA 7 cut(s) 10, 466, 591, 729, 881, 1125, 1522
AhlI ACTAGT 1 cut(s) 338
AjnI CCWGG 5 cut(s) 139, 445, 500, 520, 901
AleI CACNNNNGTG 1 cut(s) 317
AloI GAACNNNNNNTCC 2 cut(s) 258, 290
AluBI AGCT 7 cut(s) 124, 350, 417, 489, 1027, 1049, 1244
AluI AGCT 7 cut(s) 124, 350, 417, 489, 1027, 1049, 1244
Alw26I GTCTC 2 cut(s) 86, 467
AoxI GGCC 2 cut(s) 617, 1341
ApeKI GCWGC 1 cut(s) 809
ApoI RAATTY 5 cut(s) 168, 354, 723, 942, 1515
ArsI GACNNNNNNTTYG 2 cut(s) 650, 682
AseI ATTAAT 1 cut(s) 231
AspLEI GCGC 1 cut(s) 851
AspS9I GGNCC 4 cut(s) 65, 309, 382, 1457
AsuHPI GGTGA 4 cut(s) 101, 624, 1082, 1396
AsuII TTCGAA 1 cut(s) 604
AvaII GGWCC 4 cut(s) 65, 309, 382, 1457
BalI TGGCCA 1 cut(s) 619
BanI GGYRCC 1 cut(s) 848
BanII GRGCYC 1 cut(s) 629
BbrPI CACGTG 2 cut(s) 1507, 1533
BbsI GAAGAC 1 cut(s) 405
BbvI GCAGC 1 cut(s) 796
BccI CCATC 5 cut(s) 80, 637, 947, 1133, 1416
BciT130I CCWGG 5 cut(s) 141, 447, 502, 522, 903
BciVI GTATCC 1 cut(s) 427
BclI TGATCA 1 cut(s) 1332
BcoDI GTCTC 2 cut(s) 86, 467
BcuI ACTAGT 1 cut(s) 338
BfaI CTAG 5 cut(s) 125, 150, 339, 594, 1130
BfoI RGCGCY 1 cut(s) 852
BfuAI ACCTGC 1 cut(s) 961
BfuI GTATCC 1 cut(s) 427
BisI GCNGC 1 cut(s) 810
BlsI GCNGC 1 cut(s) 811
BmcAI AGTACT 1 cut(s) 343
Bme1390I CCNGG 5 cut(s) 141, 447, 502, 522, 903
Bme18I GGWCC 4 cut(s) 65, 309, 382, 1457
BmgT120I GGNCC 4 cut(s) 65, 309, 382, 1457
BmiI GGNNCC 4 cut(s) 562, 626, 850, 1459
BmrFI CCNGG 5 cut(s) 141, 447, 502, 522, 903
BmrI ACTGGG 1 cut(s) 1400
BmsI GCATC 4 cut(s) 432, 1024, 1046, 1219
BmuI ACTGGG 1 cut(s) 1400
BpiI GAAGAC 1 cut(s) 405
BplI GAGNNNNNCTC 2 cut(s) 1257, 1289
BpmI CTGGAG 3 cut(s) 523, 549, 906
Bpu14I TTCGAA 1 cut(s) 604
BsaAI YACGTR 2 cut(s) 1507, 1533
BsaHI GRCGYC 1 cut(s) 849
BsaI GGTCTC 1 cut(s) 86
BsaJI CCNNGG 4 cut(s) 139, 520, 620, 902
Bsc4I CCNNNNNNNGG 3 cut(s) 527, 1350, 1454
Bse1I ACTGG 6 cut(s) 73, 197, 532, 562, 1197, 1395
Bse3DI GCAATG 1 cut(s) 1423
BseBI CCWGG 5 cut(s) 141, 447, 502, 522, 903
BseDI CCNNGG 4 cut(s) 139, 520, 620, 902
BseGI GGATG 4 cut(s) 497, 866, 1399, 1427
BseLI CCNNNNNNNGG 3 cut(s) 527, 1350, 1454
BseMI GCAATG 1 cut(s) 1423
BseMII CTCAG 5 cut(s) 876, 927, 1227, 1236, 1288
BseNI ACTGG 6 cut(s) 73, 197, 532, 562, 1197, 1395
BseXI GCAGC 1 cut(s) 796
BsgI GTGCAG 1 cut(s) 795
BshFI GGCC 2 cut(s) 619, 1343
BshNI GGYRCC 1 cut(s) 848
BslFI GGGAC 3 cut(s) 233, 1218, 1443
BslI CCNNNNNNNGG 3 cut(s) 527, 1350, 1454
BsmAI GTCTC 2 cut(s) 86, 467
BsmFI GGGAC 3 cut(s) 233, 1218, 1443
BsmI GAATGC 1 cut(s) 1293
BsnI GGCC 2 cut(s) 619, 1343
Bso31I GGTCTC 1 cut(s) 86
Bsp119I TTCGAA 1 cut(s) 604
Bsp1286I GDGCHC 1 cut(s) 629
Bsp143I GATC 1 cut(s) 1332
Bsp19I CCATGG 1 cut(s) 620
BspACI CCGC 2 cut(s) 1455, 1502
BspANI GGCC 2 cut(s) 619, 1343
BspCNI CTCAG 5 cut(s) 877, 928, 1228, 1237, 1287
BspLI GGNNCC 4 cut(s) 562, 626, 850, 1459
BspMI ACCTGC 1 cut(s) 961
BspT104I TTCGAA 1 cut(s) 604
BspT107I GGYRCC 1 cut(s) 848
BspTNI GGTCTC 1 cut(s) 86
BsrDI GCAATG 1 cut(s) 1423
BsrI ACTGG 6 cut(s) 73, 197, 532, 562, 1197, 1395
BssECI CCNNGG 4 cut(s) 139, 520, 620, 902
BssMI GATC 1 cut(s) 1332
BssNI GRCGYC 1 cut(s) 849
BssT1I CCWWGG 1 cut(s) 620
Bst2UI CCWGG 5 cut(s) 141, 447, 502, 522, 903
Bst4CI ACNGT 1 cut(s) 395
Bst6I CTCTTC 1 cut(s) 38
BstACI GRCGYC 1 cut(s) 849
BstBAI YACGTR 2 cut(s) 1507, 1533
BstBI TTCGAA 1 cut(s) 604
BstC8I GCNNGC 3 cut(s) 1093, 1293, 1320
BstDEI CTNAG 5 cut(s) 885, 936, 1236, 1245, 1274
BstDSI CCRYGG 1 cut(s) 620
BstF5I GGATG 4 cut(s) 497, 866, 1399, 1427
BstH2I RGCGCY 1 cut(s) 852
BstHHI GCGC 1 cut(s) 851
BstKTI GATC 1 cut(s) 1335
BstMAI GTCTC 2 cut(s) 86, 467
BstMBI GATC 1 cut(s) 1332
BstMWI GCNNNNNNNGC 2 cut(s) 135, 1510
BstNI CCWGG 5 cut(s) 141, 447, 502, 522, 903
BstNSI RCATGY 3 cut(s) 1022, 1095, 1481
BstSCI CCNGG 5 cut(s) 139, 445, 500, 520, 901
BstV1I GCAGC 1 cut(s) 796
BstV2I GAAGAC 1 cut(s) 405
BstXI CCANNNNNNTGG 3 cut(s) 204, 930, 1533
BsuI GTATCC 1 cut(s) 427
BsuRI GGCC 2 cut(s) 619, 1343
BtgI CCRYGG 1 cut(s) 620
BtsCI GGATG 4 cut(s) 497, 866, 1399, 1427
BtsIMutI CAGTG 3 cut(s) 204, 400, 862
BveI ACCTGC 1 cut(s) 961
Cac8I GCNNGC 3 cut(s) 1093, 1293, 1320
CfoI GCGC 1 cut(s) 851
Cfr13I GGNCC 4 cut(s) 65, 309, 382, 1457
CseI GACGC 1 cut(s) 1102
Csp6I GTAC 3 cut(s) 342, 639, 980
CspCI CAANNNNNGTGG 2 cut(s) 717, 752
CviAII CATG 9 cut(s) 621, 771, 1019, 1092, 1110, 1156, 1161, 1336, 1478
CviQI GTAC 3 cut(s) 342, 639, 980
DdeI CTNAG 5 cut(s) 885, 936, 1236, 1245, 1274
DinI GGCGCC 1 cut(s) 850
DpnI GATC 1 cut(s) 1334
DpnII GATC 1 cut(s) 1332
DrdI GACNNNNNNGTC 1 cut(s) 100
DseDI GACNNNNNNGTC 1 cut(s) 100
EaeI YGGCCR 1 cut(s) 617
Eam1104I CTCTTC 1 cut(s) 38
EarI CTCTTC 1 cut(s) 38
Eco130I CCWWGG 1 cut(s) 620
Eco24I GRGCYC 1 cut(s) 629
Eco31I GGTCTC 1 cut(s) 86
Eco47I GGWCC 4 cut(s) 65, 309, 382, 1457
Eco57I CTGAAG 3 cut(s) 330, 743, 838
Eco72I CACGTG 2 cut(s) 1507, 1533
EcoRI GAATTC 1 cut(s) 168
EcoRII CCWGG 5 cut(s) 139, 445, 500, 520, 901
EcoT14I CCWWGG 1 cut(s) 620
EcoT22I ATGCAT 2 cut(s) 655, 1039
EcoT38I GRGCYC 1 cut(s) 629
EgeI GGCGCC 1 cut(s) 850
EheI GGCGCC 1 cut(s) 850
ErhI CCWWGG 1 cut(s) 620
FaeI CATG 9 cut(s) 624, 774, 1022, 1095, 1113, 1159, 1164, 1339, 1481
FalI AAGNNNNNCTT 2 cut(s) 281, 313
FaqI GGGAC 3 cut(s) 233, 1218, 1443
FatI CATG 9 cut(s) 620, 770, 1018, 1091, 1109, 1155, 1160, 1335, 1477
FauI CCCGC 1 cut(s) 1509
FauNDI CATATG 1 cut(s) 655
FbaI TGATCA 1 cut(s) 1332
Fnu4HI GCNGC 1 cut(s) 810
FokI GGATG 4 cut(s) 484, 873, 1386, 1434
FriOI GRGCYC 1 cut(s) 629
Fsp4HI GCNGC 1 cut(s) 810
FspBI CTAG 5 cut(s) 125, 150, 339, 594, 1130
GlaI GCGC 1 cut(s) 850
GluI GCNGC 1 cut(s) 810
GsuI CTGGAG 3 cut(s) 523, 549, 906
HaeII RGCGCY 1 cut(s) 852
HaeIII GGCC 2 cut(s) 619, 1343
HgaI GACGC 1 cut(s) 1102
HhaI GCGC 1 cut(s) 851
Hin1I GRCGYC 1 cut(s) 849
Hin1II CATG 9 cut(s) 624, 774, 1022, 1095, 1113, 1159, 1164, 1339, 1481
Hin6I GCGC 1 cut(s) 849
HinP1I GCGC 1 cut(s) 849
HincII GTYRAC 2 cut(s) 82, 742
HindII GTYRAC 2 cut(s) 82, 742
HindIII AAGCTT 2 cut(s) 415, 1025
HinfI GANTC 4 cut(s) 236, 466, 701, 1238
HpaI GTTAAC 1 cut(s) 742
HphI GGTGA 4 cut(s) 101, 624, 1082, 1396
Hpy166II GTNNAC 6 cut(s) 82, 312, 742, 1180, 1397, 1536
Hpy188I TCNGA 9 cut(s) 228, 325, 511, 721, 886, 937, 952, 1277, 1332
Hpy8I GTNNAC 6 cut(s) 82, 312, 742, 1180, 1397, 1536
Hpy99I CGWCG 1 cut(s) 1118
HpyAV CCTTC 5 cut(s) 38, 790, 862, 1272, 1360
HpyCH4III ACNGT 1 cut(s) 395
HpyCH4IV ACGT 2 cut(s) 1506, 1532
HpyCH4V TGCA 8 cut(s) 45, 653, 812, 1037, 1083, 1155, 1232, 1379
HpyF10VI GCNNNNNNNGC 2 cut(s) 135, 1510
HpyF3I CTNAG 5 cut(s) 885, 936, 1236, 1245, 1274
HpySE526I ACGT 2 cut(s) 1506, 1532
Hsp92I GRCGYC 1 cut(s) 849
Hsp92II CATG 9 cut(s) 624, 774, 1022, 1095, 1113, 1159, 1164, 1339, 1481
HspAI GCGC 1 cut(s) 849
KasI GGCGCC 1 cut(s) 848
Ksp22I TGATCA 1 cut(s) 1332
KspAI GTTAAC 1 cut(s) 742
Kzo9I GATC 1 cut(s) 1332
LmnI GCTCC 2 cut(s) 566, 624
Lsp1109I GCAGC 1 cut(s) 796
LweI GCATC 4 cut(s) 432, 1024, 1046, 1219
MaeI CTAG 5 cut(s) 125, 150, 339, 594, 1130
MaeII ACGT 2 cut(s) 1506, 1532
MaeIII GTNAC 3 cut(s) 389, 685, 827
MalI GATC 1 cut(s) 1334
MboI GATC 1 cut(s) 1332
MboII GAAGA 5 cut(s) 11, 25, 410, 524, 696
MfeI CAATTG 1 cut(s) 1410
MhlI GDGCHC 1 cut(s) 629
MlsI TGGCCA 1 cut(s) 619
MluNI TGGCCA 1 cut(s) 619
Mly113I GGCGCC 1 cut(s) 849
MlyI GAGTC 1 cut(s) 1247
MmeI TCCRAC 2 cut(s) 145, 452
Mox20I TGGCCA 1 cut(s) 619
Mph1103I ATGCAT 2 cut(s) 655, 1039
MscI TGGCCA 1 cut(s) 619
MseI TTAA 4 cut(s) 231, 299, 741, 1187
MslI CAYNNNNRTG 3 cut(s) 317, 1064, 1531
Msp20I TGGCCA 1 cut(s) 619
MspA1I CMGCKG 1 cut(s) 1244
MspR9I CCNGG 5 cut(s) 141, 447, 502, 522, 903
MunI CAATTG 1 cut(s) 1410
Mva1269I GAATGC 1 cut(s) 1293
MvaI CCWGG 5 cut(s) 141, 447, 502, 522, 903
MwoI GCNNNNNNNGC 2 cut(s) 135, 1510
NarI GGCGCC 1 cut(s) 849
NcoI CCATGG 1 cut(s) 620
NdeI CATATG 1 cut(s) 655
NdeII GATC 1 cut(s) 1332
NlaIII CATG 9 cut(s) 624, 774, 1022, 1095, 1113, 1159, 1164, 1339, 1481
NlaIV GGNNCC 4 cut(s) 562, 626, 850, 1459
NmuCI GTSAC 2 cut(s) 685, 827
NsiI ATGCAT 2 cut(s) 655, 1039
NspI RCATGY 3 cut(s) 1022, 1095, 1481
NspV TTCGAA 1 cut(s) 604
OliI CACNNNNGTG 1 cut(s) 317
PaeI GCATGC 1 cut(s) 1095
PciI ACATGT 1 cut(s) 1018
PctI GAATGC 1 cut(s) 1293
PfeI GAWTC 3 cut(s) 236, 466, 701
PfoI TCCNGGA 1 cut(s) 500
PkrI GCNGC 1 cut(s) 811
PleI GAGTC 1 cut(s) 1246
PluTI GGCGCC 1 cut(s) 852
PmaCI CACGTG 2 cut(s) 1507, 1533
PmlI CACGTG 2 cut(s) 1507, 1533
PpsI GAGTC 1 cut(s) 1246
Ppu21I YACGTR 2 cut(s) 1507, 1533
PscI ACATGT 1 cut(s) 1018
PshBI ATTAAT 1 cut(s) 231
PsiI TTATAA 2 cut(s) 288, 1443
Psp6I CCWGG 5 cut(s) 139, 445, 500, 520, 901
PspCI CACGTG 2 cut(s) 1507, 1533
PspGI CCWGG 5 cut(s) 139, 445, 500, 520, 901
PspN4I GGNNCC 4 cut(s) 562, 626, 850, 1459
PspPI GGNCC 4 cut(s) 65, 309, 382, 1457
PvuII CAGCTG 1 cut(s) 1244
RsaI GTAC 3 cut(s) 343, 640, 981
RsaNI GTAC 3 cut(s) 342, 639, 980
RseI CAYNNNNRTG 3 cut(s) 317, 1064, 1531
SaqAI TTAA 4 cut(s) 231, 299, 741, 1187
SatI GCNGC 1 cut(s) 810
Sau3AI GATC 1 cut(s) 1332
Sau96I GGNCC 4 cut(s) 65, 309, 382, 1457
ScaI AGTACT 1 cut(s) 343
SchI GAGTC 1 cut(s) 1247
ScrFI CCNGG 5 cut(s) 141, 447, 502, 522, 903
SduI GDGCHC 1 cut(s) 629
SfaNI GCATC 4 cut(s) 432, 1024, 1046, 1219
SfoI GGCGCC 1 cut(s) 850
SfuI TTCGAA 1 cut(s) 604
SinI GGWCC 4 cut(s) 65, 309, 382, 1457
SmiMI CAYNNNNRTG 3 cut(s) 317, 1064, 1531
SpeI ACTAGT 1 cut(s) 338
SphI GCATGC 1 cut(s) 1095
SsiI CCGC 2 cut(s) 1455, 1502
SspDI GGCGCC 1 cut(s) 848
SspMI CTAG 5 cut(s) 125, 150, 339, 594, 1130
StyD4I CCNGG 5 cut(s) 139, 445, 500, 520, 901
StyI CCWWGG 1 cut(s) 620
TaaI ACNGT 1 cut(s) 395
TaiI ACGT 2 cut(s) 1509, 1535
TaqI TCGA 2 cut(s) 604, 1222
TatI WGTACW 2 cut(s) 341, 638
TfiI GAWTC 3 cut(s) 236, 466, 701
Tru1I TTAA 4 cut(s) 231, 299, 741, 1187
Tru9I TTAA 4 cut(s) 231, 299, 741, 1187
TscAI CASTG 3 cut(s) 204, 400, 862
TseFI GTSAC 2 cut(s) 685, 827
TseI GCWGC 1 cut(s) 809
Tsp45I GTSAC 2 cut(s) 685, 827
TspDTI ATGAA 7 cut(s) 128, 228, 467, 481, 528, 562, 1098
TspGWI ACGGA 2 cut(s) 446, 852
TspRI CASTG 3 cut(s) 204, 400, 862
VpaK11BI GGWCC 4 cut(s) 65, 309, 382, 1457
VspI ATTAAT 1 cut(s) 231
XapI RAATTY 5 cut(s) 168, 354, 723, 942, 1515
XbaI TCTAGA 1 cut(s) 1129
XceI RCATGY 3 cut(s) 1022, 1095, 1481
XspI CTAG 5 cut(s) 125, 150, 339, 594, 1130
ZrmI AGTACT 1 cut(s) 343
Zsp2I ATGCAT 2 cut(s) 655, 1039
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.