RchiOBHm_Chr7g0224121

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000112
Physical Location & Seq
Reverse (-)
45983676 .. 45987427
3752 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20067

Sequence Viewer

Length: 2433 bp
ATGTTACTGAAAGCTCTGCTTCTCATCCTCCTCTTCCAACTGTCCACTTCCAAGGACACCATAACATGGAACGGAGAGCAAGTAAAGGATGGTGACCTTCTAGTGTCCAAAGGAAACGACTTTGAATTGGGTTTCTTCAGCCCCAGAAACTCGAGCTACCGCTATGTTGGAATTTGGTATTCCATGTCTCAGATATCTGACCAAACAGTGGTGTGGGTGGCAAACAGAAACAACCCCATCAATGATACATCAGGTGTTCTCACAATAAACAGGTATGGAAAACTAGTCATTTATGCTCACAATATGGAGAACGTTCCTATATGGTCTGCCAATATGTCTCGGTCAGTCATAAAAACAAACCTCTTATCTGCACAACTTTTAGATACAGGAAACTTAGTTCTGACCCAGGATGGTAAAACTGAAAGCTTTATTATATGGCAGAGTTTTGATTATCCTACAGATACTCTAATCCCTGGTATGAAACTTGGGGTGAGTTGGAAAACTGGTTTAGAGTGGTTTTTAACATCTTGGAAGTCACCAGATGACCCTGGAACTGGGGACTATAGCCATAGGCTTCATTGGAACCAGACGGCCATGCTACAATATTTTCTGTTCAAGGGTTTAACTACTAGGGTTTGGAGAAGTGATCCTGGGCCTTGGCGCAATTTTGTTAGTAATAATGATGAAACGTATTACCTACAATCTAGTGATAACTCCACTATAAAATTTAGAATGGTTGTGAATGATTCTGGGTTATTGCAGCATTTGACATGGAATGATGGTGACCATCAATGGAAGGAACTATGGGCTGCACCGAAATACCGTTGTGACTTGTATGGACAGTGTGGTGCCTATAGTAAATGTAACCCTGACAATATTAATATGTTTGAGTGTGAGTGTTTACCAGGGTATGAACCTAAATCTATAAATGACTGGAATCAGAATGATGGGTCAGAGGGGTGTCTGAGTAAGCGAGTTGGAGTGTCAAAGTGTGGAGTTGGAGATGGGTTTTTAGAGGTGGCAAGGGTGAAAGCTCCAGACACATCGACAGTAGTAGCACGACTGGATACTGGTATGAGTGCCAAAGAGTGCAAGCAGGAATGTTTGAGAAATTGTTCTTGCACAGCATGTATGAGCATCAATAATAAAGGGCGCATTGATTGCTTGACATGGTATGGTGAGCTGCTGGATATTGTGAAGCACACGGATGAAGGACAAGTTCTACATGTTCGTGTGGATGAAATTGAGTTAGCTATGTATTCCAGAAAATCAGAAGGTTTTTTGGAAAGGAGGGGCATGCTGGCTATTCCAATATTGTCTGGTCTACTGGTGCTGGTACTAATCCCTGTGTTTGGCTGTTGGTGGCTTAAGAAAAGAAGGAAAACCAAAGACTTAGCAGTGGTGGATGAACTTGGAAAAGCTAGGAAACACCCTGATCTGCAATTTTTCTCTCTGAGCACAATAATTGTAGCCACAGGCAACTTCCATTCTGGAAATAAACTCGGGGAAGGTGGTTTTGGATCTGTTTATAAGGGTCAGTTACCAAATGGACAGGAGATTGCTGTGAAAAGATTGTCCAAAACTTCAGGGCAAGGGATTGAAGAATTCAAAAATGAAGTTGTGCTTATAGAAAGACTTCAACACAGGAACCTTGTGAAACTTTTGGGTTGTTGCATAGCGGGAGAAGAAAAGATGTTGGTCCTTGAATACTTGTCCAACAAAAGCTTGGACTTCTTTCTTTTTGATGACACAAGAAGGTCCTCTTTGGGTTGGAAAAAGCGCTTTGAAATCATCAATGGGATTGCTCGTGGGATTCTTTATCTTCACCAGGACTCGAGAGTTAGAATTATACATAGGGATCTAAAAACCAGCAATATTCTTCTAGATGCTGAGATGCACCCAAAAATTTCAGATTTTGGCATGGCAAGAATATTTTATGGGGACCAATTGCAGGATAAGACAGCCAGAGTTGTTGGAACATATGGGTATATGTCACCAGAGTATGCAGTTTTTGGTAGATATTCAACAAAATCAGATGTCTTTAGCTTCGGGATCATAATATTGGAGATCATAAGCGGCAAGAAAAACTCTGGTTCATATTGGGAGGATCATTCCATGAACTTGATAGGAAATGTTTGGCAGATGTGGGGTGAAGACAGAGCTTTAGAAATTATGGATTCATCACTAGAGTTATATCAACCAGATGAAGTCTTGAGATGCATTCAAGTTGGGCTCTTATGTGTGCAAGAAGATTCAAAGGACCGGCCTACCATGTCAGATGCTGTTTTCATGTTGAGTGGTGAAGCATCTCTTTCAACTCCTAAGCAGCCTGCATTTGTTTTTAGAAAAAGTGCATGCAGTAATGTTCCATCCCTTGCAAGTGGATTATGTTCAATAAATGACTTAACAATAACCAATGTGGATGTTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

810

Amino Acids

91.5

Weight (kDa)

6.56

Isoelectric Point (pI)

38.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 69 - 178 5.7e-35 D-mannose binding lectin
S_locus_glycop PF00954 222 - 308 1.6e-18 S-locus glycoprotein domain
PAN_2 PF08276 331 - 398 1.1e-15 PAN-like domain
Pkinase PF00069 495 - 761 2.3e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 497 - 761 5e-49 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1530
AccB1I GGYRCC 1 cut(s) 848
AccB7I CCANNNNNTGG 2 cut(s) 66, 208
AccI GTMKAC 1 cut(s) 1324
AciI CCGC 3 cut(s) 160, 1679, 2076
AclI AACGTT 1 cut(s) 312
AclWI GGATC 5 cut(s) 641, 1528, 1866, 2060, 2115
AcoI YGGCCR 1 cut(s) 591
AcsI RAATTY 4 cut(s) 171, 725, 1604, 1905
AcuI CTGAAG 2 cut(s) 121, 1569
AfaI GTAC 1 cut(s) 1338
AfeI AGCGCT 1 cut(s) 1781
AfiI CCNNNNNNNGG 5 cut(s) 66, 208, 554, 1352, 1951
AflII CTTAAG 1 cut(s) 1367
AflIII ACRYGT 1 cut(s) 1225
AhlI ACTAGT 1 cut(s) 283
AjnI CCWGG 6 cut(s) 405, 472, 547, 649, 904, 1827
AjuI GAANNNNNNNTTGG 2 cut(s) 1500, 1532
AloI GAACNNNNNNTCC 2 cut(s) 381, 413
Alw21I GWGCWC 1 cut(s) 1460
Alw26I GTCTC 2 cut(s) 192, 342
AlwI GGATC 5 cut(s) 641, 1528, 1866, 2060, 2115
AlwNI CAGNNNCTG 1 cut(s) 2282
Ama87I CYCGRG 3 cut(s) 151, 1502, 1834
Aor51HI AGCGCT 1 cut(s) 1781
AoxI GGCC 3 cut(s) 591, 653, 2264
ApeKI GCWGC 4 cut(s) 760, 809, 1183, 2326
ApoI RAATTY 4 cut(s) 171, 725, 1604, 1905
AseI ATTAAT 1 cut(s) 879
Asp700I GAANNNNTTC 2 cut(s) 1114, 1635
AspLEI GCGC 3 cut(s) 663, 1155, 1782
AspS9I GGNCC 5 cut(s) 653, 1699, 1758, 1942, 2260
AvaI CYCGRG 3 cut(s) 151, 1502, 1834
AvaII GGWCC 4 cut(s) 1699, 1758, 1942, 2260
BanI GGYRCC 1 cut(s) 848
BanII GRGCYC 1 cut(s) 2235
BauI CACGAG 1 cut(s) 1806
BbsI GAAGAC 1 cut(s) 2160
Bbv12I GWGCWC 1 cut(s) 1460
BbvI GCAGC 4 cut(s) 772, 796, 1170, 2338
BccI CCATC 8 cut(s) 83, 245, 404, 773, 795, 941, 998, 2377
BceAI ACGGC 1 cut(s) 606
BciT130I CCWGG 6 cut(s) 407, 474, 549, 651, 906, 1829
BciVI GTATCC 1 cut(s) 1060
BcoDI GTCTC 2 cut(s) 192, 342
BcuI ACTAGT 1 cut(s) 283
BfaI CTAG 7 cut(s) 101, 284, 630, 705, 1422, 1883, 2186
BfmI CTRYAG 3 cut(s) 456, 562, 853
BfoI RGCGCY 1 cut(s) 1783
BfrI CTTAAG 1 cut(s) 1367
BfuI GTATCC 1 cut(s) 1060
BisI GCNGC 5 cut(s) 761, 810, 1184, 2077, 2327
BlsI GCNGC 5 cut(s) 762, 811, 1185, 2078, 2328
Bme1390I CCNGG 6 cut(s) 407, 474, 549, 651, 906, 1829
Bme18I GGWCC 4 cut(s) 1699, 1758, 1942, 2260
BmeT110I CYCGRG 3 cut(s) 151, 1502, 1834
BmgT120I GGNCC 5 cut(s) 653, 1699, 1758, 1942, 2260
BmiI GGNNCC 4 cut(s) 584, 850, 1649, 1943
BmrFI CCNGG 6 cut(s) 407, 474, 549, 651, 906, 1829
BmrI ACTGGG 1 cut(s) 564
BmsI GCATC 6 cut(s) 1146, 1876, 1884, 2207, 2269, 2315
BmuI ACTGGG 1 cut(s) 564
BpiI GAAGAC 1 cut(s) 2160
BpmI CTGGAG 1 cut(s) 1020
Bpu10I CCTNAGC 1 cut(s) 2322
BpuEI CTTGAG 1 cut(s) 2233
BsaJI CCNNGG 7 cut(s) 51, 405, 472, 547, 650, 656, 905
BsaXI ACNNNNNCTCC 2 cut(s) 66, 96
Bsc4I CCNNNNNNNGG 5 cut(s) 66, 208, 554, 1352, 1951
Bse118I RCCGGY 1 cut(s) 2262
Bse1I ACTGG 6 cut(s) 508, 559, 938, 1068, 1075, 1332
BseBI CCWGG 6 cut(s) 407, 474, 549, 651, 906, 1829
BseDI CCNNGG 7 cut(s) 51, 405, 472, 547, 650, 656, 905
BseGI GGATG 8 cut(s) 24, 94, 415, 1213, 1243, 1411, 2369, 2428
BseLI CCNNNNNNNGG 5 cut(s) 66, 208, 554, 1352, 1951
BseMII CTCAG 4 cut(s) 203, 956, 1445, 1881
BseNI ACTGG 6 cut(s) 508, 559, 938, 1068, 1075, 1332
BseRI GAGGAG 1 cut(s) 20
BseXI GCAGC 4 cut(s) 772, 796, 1170, 2338
BsgI GTGCAG 2 cut(s) 354, 795
BshFI GGCC 3 cut(s) 593, 655, 2266
BshNI GGYRCC 1 cut(s) 848
BsiHKAI GWGCWC 1 cut(s) 1460
BsiHKCI CYCGRG 3 cut(s) 151, 1502, 1834
BsiSI CCGG 1 cut(s) 2263
BslFI GGGAC 2 cut(s) 572, 1955
BslI CCNNNNNNNGG 5 cut(s) 66, 208, 554, 1352, 1951
BsmAI GTCTC 2 cut(s) 192, 342
BsmFI GGGAC 2 cut(s) 572, 1955
BsmI GAATGC 1 cut(s) 2220
BsnI GGCC 3 cut(s) 593, 655, 2266
BsoBI CYCGRG 3 cut(s) 151, 1502, 1834
Bsp1286I GDGCHC 2 cut(s) 1460, 2235
Bsp143I GATC 7 cut(s) 646, 1435, 1520, 1858, 2052, 2067, 2107
BspACI CCGC 3 cut(s) 160, 1679, 2076
BspANI GGCC 3 cut(s) 593, 655, 2266
BspCNI CTCAG 4 cut(s) 202, 957, 1446, 1882
BspLI GGNNCC 4 cut(s) 584, 850, 1649, 1943
BspPI GGATC 5 cut(s) 641, 1528, 1866, 2060, 2115
BspT107I GGYRCC 1 cut(s) 848
BspTI CTTAAG 1 cut(s) 1367
BsrFI RCCGGY 1 cut(s) 2262
BsrI ACTGG 6 cut(s) 508, 559, 938, 1068, 1075, 1332
BssAI RCCGGY 1 cut(s) 2262
BssECI CCNNGG 7 cut(s) 51, 405, 472, 547, 650, 656, 905
BssMI GATC 7 cut(s) 646, 1435, 1520, 1858, 2052, 2067, 2107
BssSI CACGAG 1 cut(s) 1806
BssT1I CCWWGG 2 cut(s) 51, 656
Bst2BI CACGAG 1 cut(s) 1806
Bst2UI CCWGG 6 cut(s) 407, 474, 549, 651, 906, 1829
Bst4CI ACNGT 5 cut(s) 42, 208, 824, 843, 1051
Bst6I CTCTTC 1 cut(s) 38
BstAFI CTTAAG 1 cut(s) 1367
BstAPI GCANNNNNTGC 1 cut(s) 1161
BstC8I GCNNGC 5 cut(s) 1094, 1298, 1302, 2331, 2356
BstDEI CTNAG 7 cut(s) 189, 394, 965, 1393, 1454, 1890, 2322
BstEII GGTNACC 2 cut(s) 92, 782
BstF5I GGATG 8 cut(s) 24, 94, 415, 1213, 1243, 1411, 2369, 2428
BstH2I RGCGCY 1 cut(s) 1783
BstHHI GCGC 3 cut(s) 663, 1155, 1782
BstKTI GATC 7 cut(s) 649, 1438, 1523, 1861, 2055, 2070, 2110
BstMAI GTCTC 2 cut(s) 192, 342
BstMBI GATC 7 cut(s) 646, 1435, 1520, 1858, 2052, 2067, 2107
BstMWI GCNNNNNNNGC 1 cut(s) 1161
BstNI CCWGG 6 cut(s) 407, 474, 549, 651, 906, 1829
BstNSI RCATGY 4 cut(s) 1131, 1229, 1300, 2358
BstPI GGTNACC 2 cut(s) 92, 782
BstSCI CCNGG 6 cut(s) 405, 472, 547, 649, 904, 1827
BstSFI CTRYAG 3 cut(s) 456, 562, 853
BstV1I GCAGC 4 cut(s) 772, 796, 1170, 2338
BstV2I GAAGAC 1 cut(s) 2160
BstX2I RGATCY 2 cut(s) 1520, 1858
BstYI RGATCY 2 cut(s) 1520, 1858
BsuI GTATCC 1 cut(s) 1060
BsuRI GGCC 3 cut(s) 593, 655, 2266
BtsCI GGATG 8 cut(s) 24, 94, 415, 1213, 1243, 1411, 2369, 2428
BtsI GCAGTG 1 cut(s) 1404
BtsIMutI CAGTG 3 cut(s) 213, 848, 1404
Cac8I GCNNGC 5 cut(s) 1094, 1298, 1302, 2331, 2356
CaiI CAGNNNCTG 1 cut(s) 2282
CfoI GCGC 3 cut(s) 663, 1155, 1782
Cfr10I RCCGGY 1 cut(s) 2262
Cfr13I GGNCC 5 cut(s) 653, 1699, 1758, 1942, 2260
Csp6I GTAC 1 cut(s) 1337
CviQI GTAC 1 cut(s) 1337
DdeI CTNAG 7 cut(s) 189, 394, 965, 1393, 1454, 1890, 2322
DpnI GATC 7 cut(s) 648, 1437, 1522, 1860, 2054, 2069, 2109
DpnII GATC 7 cut(s) 646, 1435, 1520, 1858, 2052, 2067, 2107
EaeI YGGCCR 1 cut(s) 591
Eam1104I CTCTTC 1 cut(s) 38
EarI CTCTTC 1 cut(s) 38
Eco130I CCWWGG 2 cut(s) 51, 656
Eco24I GRGCYC 1 cut(s) 2235
Eco32I GATATC 1 cut(s) 195
Eco47I GGWCC 4 cut(s) 1699, 1758, 1942, 2260
Eco47III AGCGCT 1 cut(s) 1781
Eco57I CTGAAG 2 cut(s) 121, 1569
Eco88I CYCGRG 3 cut(s) 151, 1502, 1834
Eco91I GGTNACC 2 cut(s) 92, 782
EcoO109I RGGNCCY 1 cut(s) 1758
EcoO65I GGTNACC 2 cut(s) 92, 782
EcoRI GAATTC 1 cut(s) 1604
EcoRII CCWGG 6 cut(s) 405, 472, 547, 649, 904, 1827
EcoRV GATATC 1 cut(s) 195
EcoT14I CCWWGG 2 cut(s) 51, 656
EcoT22I ATGCAT 1 cut(s) 2222
EcoT38I GRGCYC 1 cut(s) 2235
ErhI CCWWGG 2 cut(s) 51, 656
FalI AAGNNNNNCTT 7 cut(s) 35, 1608, 1640, 1747, 1779, 2295, 2327
FaqI GGGAC 2 cut(s) 572, 1955
FauI CCCGC 1 cut(s) 1672
FauNDI CATATG 1 cut(s) 1981
FblI GTMKAC 1 cut(s) 1324
Fnu4HI GCNGC 5 cut(s) 761, 810, 1184, 2077, 2327
FokI GGATG 7 cut(s) 11, 101, 422, 1220, 1250, 1418, 2356
FriOI GRGCYC 1 cut(s) 2235
Fsp4HI GCNGC 5 cut(s) 761, 810, 1184, 2077, 2327
FspBI CTAG 7 cut(s) 101, 284, 630, 705, 1422, 1883, 2186
GlaI GCGC 3 cut(s) 662, 1154, 1781
GluI GCNGC 5 cut(s) 761, 810, 1184, 2077, 2327
GsuI CTGGAG 1 cut(s) 1020
HaeII RGCGCY 1 cut(s) 1783
HaeIII GGCC 3 cut(s) 593, 655, 2266
HapII CCGG 1 cut(s) 2263
HhaI GCGC 3 cut(s) 663, 1155, 1782
Hin6I GCGC 3 cut(s) 661, 1153, 1780
HinP1I GCGC 3 cut(s) 661, 1153, 1780
HindIII AAGCTT 2 cut(s) 424, 1723
HinfI GANTC 6 cut(s) 746, 937, 1813, 1832, 2177, 2252
HpaII CCGG 1 cut(s) 2263
Hpy166II GTNNAC 3 cut(s) 45, 902, 1325
Hpy188III TCNNGA 7 cut(s) 1037, 1263, 1491, 1836, 1883, 2050, 2212
Hpy8I GTNNAC 3 cut(s) 45, 902, 1325
HpyAV CCTTC 7 cut(s) 107, 790, 1205, 1268, 1371, 1502, 1749
HpyCH4III ACNGT 5 cut(s) 42, 208, 824, 843, 1051
HpyCH4IV ACGT 2 cut(s) 312, 689
HpyF10VI GCNNNNNNNGC 1 cut(s) 1161
HpyF3I CTNAG 7 cut(s) 189, 394, 965, 1393, 1454, 1890, 2322
HpySE526I ACGT 2 cut(s) 312, 689
HspAI GCGC 3 cut(s) 661, 1153, 1780
Kzo9I GATC 7 cut(s) 646, 1435, 1520, 1858, 2052, 2067, 2107
LmnI GCTCC 1 cut(s) 1039
Lsp1109I GCAGC 4 cut(s) 772, 796, 1170, 2338
LweI GCATC 6 cut(s) 1146, 1876, 1884, 2207, 2269, 2315
MaeI CTAG 7 cut(s) 101, 284, 630, 705, 1422, 1883, 2186
MaeII ACGT 2 cut(s) 312, 689
MaeIII GTNAC 8 cut(s) 3, 92, 534, 782, 827, 863, 1539, 1992
MalI GATC 7 cut(s) 648, 1437, 1522, 1860, 2054, 2069, 2109
MboI GATC 7 cut(s) 646, 1435, 1520, 1858, 2052, 2067, 2107
MboII GAAGA 8 cut(s) 25, 127, 1613, 1697, 1814, 1871, 2165, 2261
MfeI CAATTG 1 cut(s) 1946
MflI RGATCY 2 cut(s) 1520, 1858
MhlI GDGCHC 2 cut(s) 1460, 2235
MlyI GAGTC 1 cut(s) 1826
MmeI TCCRAC 8 cut(s) 61, 148, 476, 958, 979, 1740, 1751, 1954
MnlI CCTC 8 cut(s) 38, 41, 371, 949, 1009, 1284, 1771, 2098
Mph1103I ATGCAT 1 cut(s) 2222
MroXI GAANNNNTTC 2 cut(s) 1114, 1635
MseI TTAA 5 cut(s) 521, 623, 879, 1368, 2405
MslI CAYNNNNRTG 2 cut(s) 1206, 1230
MspCI CTTAAG 1 cut(s) 1367
MspI CCGG 1 cut(s) 2263
MspR9I CCNGG 6 cut(s) 407, 474, 549, 651, 906, 1829
MunI CAATTG 1 cut(s) 1946
Mva1269I GAATGC 1 cut(s) 2220
MvaI CCWGG 6 cut(s) 407, 474, 549, 651, 906, 1829
MwoI GCNNNNNNNGC 1 cut(s) 1161
NdeI CATATG 1 cut(s) 1981
NdeII GATC 7 cut(s) 646, 1435, 1520, 1858, 2052, 2067, 2107
NlaIV GGNNCC 4 cut(s) 584, 850, 1649, 1943
NmuCI GTSAC 5 cut(s) 92, 534, 782, 827, 1992
NsiI ATGCAT 1 cut(s) 2222
NspI RCATGY 4 cut(s) 1131, 1229, 1300, 2358
PaeI GCATGC 2 cut(s) 1300, 2358
PaeR7I CTCGAG 2 cut(s) 151, 1834
PciI ACATGT 1 cut(s) 1225
PctI GAATGC 1 cut(s) 2220
PdmI GAANNNNTTC 2 cut(s) 1114, 1635
PfeI GAWTC 5 cut(s) 746, 937, 1813, 2177, 2252
PflMI CCANNNNNTGG 2 cut(s) 66, 208
PkrI GCNGC 5 cut(s) 762, 811, 1185, 2078, 2328
PleI GAGTC 1 cut(s) 1826
PpsI GAGTC 1 cut(s) 1826
PpuMI RGGWCCY 1 cut(s) 1758
PscI ACATGT 1 cut(s) 1225
PshBI ATTAAT 1 cut(s) 879
PsiI TTATAA 1 cut(s) 1530
Psp1406I AACGTT 1 cut(s) 312
Psp5II RGGWCCY 1 cut(s) 1758
Psp6I CCWGG 6 cut(s) 405, 472, 547, 649, 904, 1827
PspEI GGTNACC 2 cut(s) 92, 782
PspGI CCWGG 6 cut(s) 405, 472, 547, 649, 904, 1827
PspN4I GGNNCC 4 cut(s) 584, 850, 1649, 1943
PspPI GGNCC 5 cut(s) 653, 1699, 1758, 1942, 2260
PspPPI RGGWCCY 1 cut(s) 1758
PspXI VCTCGAGB 1 cut(s) 151
PstNI CAGNNNCTG 1 cut(s) 2282
PsuI RGATCY 2 cut(s) 1520, 1858
RsaI GTAC 1 cut(s) 1338
RsaNI GTAC 1 cut(s) 1337
RseI CAYNNNNRTG 2 cut(s) 1206, 1230
SaqAI TTAA 5 cut(s) 521, 623, 879, 1368, 2405
SatI GCNGC 5 cut(s) 761, 810, 1184, 2077, 2327
Sau3AI GATC 7 cut(s) 646, 1435, 1520, 1858, 2052, 2067, 2107
Sau96I GGNCC 5 cut(s) 653, 1699, 1758, 1942, 2260
SchI GAGTC 1 cut(s) 1826
ScrFI CCNGG 6 cut(s) 407, 474, 549, 651, 906, 1829
SduI GDGCHC 2 cut(s) 1460, 2235
SfaNI GCATC 6 cut(s) 1146, 1876, 1884, 2207, 2269, 2315
SfcI CTRYAG 3 cut(s) 456, 562, 853
Sfr274I CTCGAG 2 cut(s) 151, 1834
SinI GGWCC 4 cut(s) 1699, 1758, 1942, 2260
SlaI CTCGAG 2 cut(s) 151, 1834
SmiMI CAYNNNNRTG 2 cut(s) 1206, 1230
SmlI CTYRAG 4 cut(s) 151, 1367, 1834, 2212
SmoI CTYRAG 4 cut(s) 151, 1367, 1834, 2212
SpeI ACTAGT 1 cut(s) 283
SphI GCATGC 2 cut(s) 1300, 2358
SsiI CCGC 3 cut(s) 160, 1679, 2076
SspI AATATT 6 cut(s) 605, 877, 1314, 1876, 1932, 2061
SspMI CTAG 7 cut(s) 101, 284, 630, 705, 1422, 1883, 2186
StyD4I CCNGG 6 cut(s) 405, 472, 547, 649, 904, 1827
StyI CCWWGG 2 cut(s) 51, 656
TaaI ACNGT 5 cut(s) 42, 208, 824, 843, 1051
TaiI ACGT 2 cut(s) 315, 692
TaqI TCGA 4 cut(s) 152, 1046, 1835, 2428
TaqII GACCGA 1 cut(s) 330
TauI GCSGC 1 cut(s) 2079
TfiI GAWTC 5 cut(s) 746, 937, 1813, 2177, 2252
Tru1I TTAA 5 cut(s) 521, 623, 879, 1368, 2405
Tru9I TTAA 5 cut(s) 521, 623, 879, 1368, 2405
TscAI CASTG 3 cut(s) 213, 848, 1404
TseFI GTSAC 5 cut(s) 92, 534, 782, 827, 1992
TseI GCWGC 4 cut(s) 760, 809, 1183, 2326
Tsp45I GTSAC 5 cut(s) 92, 534, 782, 827, 1992
TspGWI ACGGA 2 cut(s) 87, 1220
TspRI CASTG 3 cut(s) 213, 848, 1404
Van91I CCANNNNNTGG 2 cut(s) 66, 208
Vha464I CTTAAG 1 cut(s) 1367
VpaK11BI GGWCC 4 cut(s) 1699, 1758, 1942, 2260
VspI ATTAAT 1 cut(s) 879
XapI RAATTY 4 cut(s) 171, 725, 1604, 1905
XbaI TCTAGA 1 cut(s) 1882
XceI RCATGY 4 cut(s) 1131, 1229, 1300, 2358
XcmI CCANNNNNNNNNTGG 1 cut(s) 1723
XhoI CTCGAG 2 cut(s) 151, 1834
XmiI GTMKAC 1 cut(s) 1324
XmnI GAANNNNTTC 2 cut(s) 1114, 1635
XspI CTAG 7 cut(s) 101, 284, 630, 705, 1422, 1883, 2186
Zsp2I ATGCAT 1 cut(s) 2222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.