RLG00000031289

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000112
Physical Location & Seq
Forward (+)
3745719 .. 3748628
2910 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031289

Sequence Viewer

Length: 1629 bp
ATGGAGAGCACTCCTATTTGGTCTACTTATGTGTCGCGGTCGGTTCAAAATGTTAACACAAGCACTTCATCTGCACAGCTTTTAGATACAGGAAATTTAGTTGTGTTCCAGGATGATAAAAATGAAATCTTTATATGGCAAAGTTTTGATCATCCTATAGATACTTTAATTCCAGGTATGAAAGTTGGGGTGAATTGGAAAACTGGGCTACAATGGGTTTTAACATCTTGGAAGTCACAAGATGACCCTGGAACTGGGGACTATACCCATAGGCTAAGTTCAGATCATAATGCATTTCCCCAATTTTTTTTGTATAAAGGTTTGAATAAGTATTGGCGAAGTGATCCAGGCCCAGCGCCTACTTTGGTCACTAATCAAGATGAAACTTATTATTTCATGAATGAAACCAATGCAATTACAAGAATAACAGTGACTGATTCTGCCTTAGAGCGGCTTACATGGGATAATGGTGGCCTTCAATGGAAGGAAGAATTCTTTGCACCGAAGTCCCGGTGTGACAGGTACGGACAGTGTGGTGCCAACAGCAGATGTAGCCCTGACAATGTTAATCTGTTTGAGTGTGACTGTTTGCCAGGGTATGTGCCTAATTCTATAAGTGATTGGAATCAGAAAAATGGTTCGGGTGGATGTGTGAGTAATCGACTTGGTTTGTTGAAGTGTGGAGATGGAGACGGGTTTATAAAGGTGGCAAGAGTTAAATATCCAGACACATCGATAGCAGCATCGTTAAAATCAGGTATGAGCGACAAAGAGTGTGCGCAGGAGTGCCTAAGAAATTGTTCTTGCACTGCATATTTGAGCACTGAAAATGAAGGGATTGTTGATTGCTTGACATGGTATGATGACTTGATGGACATTTTAGTGTACACAGAGCTTGGACAAGATCTCTACGTTCGTGTGAATGCAACTGTGTTAGATAACACAAGACGGTCCTTCTTGGATTGGGAAAAGCGTTTTGAAATTATCAACGGGATTGCGCGTGGGATACTATATCTTCACCAAGACTCAAGATTAAGGATTATCCATAGAGATCTGAAAACTAGTAATGTTCTACTAGATGATGAGATGAACCCAAAAATTTCTGATTTTGGCATGGCTAGAATATTCCATGGGGACCAACTGCAAGATAAGACGAGCCGAATTGTCGGAACATATGGCTACATGTCACCGGAGTACGCAGTCTTTGGGAGATTTTCCACAAAATCTGATGTCTTTAGTTTTGGGATCATAATGTTGGAGATTGTAAGCGGCCAGAAAAACAGTGGCTCTGATCTGGAGGATCCTTCCATGAACTTAATAGGACATGTTTGGAAGCTCTGGAGCGAAGGCAAAGCCTTAGATATTGTGGATTCAACACTGAAGTCATGTCAGCCTAATGAAGTCCTAAGATGCATACAAGTTGCGCTCTTGTGTGTACAAGAAGATTCAAAGGACCGACCTGCCATGTCAGCCATTGTTTTCATGTTGAGTGGTGAAGCATCTCCTTCATTGCCTAAGCAGCCGGCATTTGTTTACAGAAGAAATTTCGGCACTGATGTTGATCCATTACTTTCAAACAGATCTTCTTCTATAAATGACTTGACAGTAACTACAATGGAAGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

543

Amino Acids

60.95

Weight (kDa)

5.01

Isoelectric Point (pI)

39.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 3 - 78 6.3e-22 D-mannose binding lectin
S_locus_glycop PF00954 125 - 203 1.5e-13 S-locus glycoprotein domain
PAN_2 PF08276 227 - 293 2.3e-17 PAN-like domain
Pkinase PF00069 259 - 492 3.7e-27 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 293 - 494 6.8e-28 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 701
Acc16I TGCGCA 1 cut(s) 780
Acc36I ACCTGC 1 cut(s) 1468
AccB1I GGYRCC 1 cut(s) 536
AccBSI CCGCTC 1 cut(s) 451
AccI GTMKAC 1 cut(s) 23
AccII CGCG 2 cut(s) 37, 1000
AciI CCGC 3 cut(s) 37, 451, 1269
AclWI GGATC 5 cut(s) 338, 1253, 1295, 1308, 1556
AcoI YGGCCR 1 cut(s) 1270
AcsI RAATTY 4 cut(s) 94, 491, 1098, 1543
AcuI CTGAAG 1 cut(s) 1400
AfaI GTAC 4 cut(s) 524, 887, 1196, 1437
AfiI CCNNNNNNNGG 2 cut(s) 254, 450
AflIII ACRYGT 2 cut(s) 1182, 1324
AgsI TTSAA 8 cut(s) 47, 325, 479, 676, 980, 1374, 1449, 1575
AhlI ACTAGT 1 cut(s) 1061
AjnI CCWGG 5 cut(s) 108, 172, 247, 346, 592
AluBI AGCT 4 cut(s) 79, 895, 1336, 1622
AluI AGCT 4 cut(s) 79, 895, 1336, 1622
Alw21I GWGCWC 2 cut(s) 11, 824
Alw26I GTCTC 1 cut(s) 684
AlwI GGATC 5 cut(s) 338, 1253, 1295, 1308, 1556
AlwNI CAGNNNCTG 1 cut(s) 434
AoxI GGCC 3 cut(s) 349, 472, 1270
ApeKI GCWGC 2 cut(s) 740, 1519
ApoI RAATTY 4 cut(s) 94, 491, 1098, 1543
Asp700I GAANNNNTTC 1 cut(s) 799
AspLEI GCGC 4 cut(s) 358, 781, 1000, 1426
AspS9I GGNCC 4 cut(s) 350, 951, 1135, 1453
AsuC2I CCSGG 1 cut(s) 511
AsuHPI GGTGA 4 cut(s) 202, 1010, 1179, 1505
AvaII GGWCC 3 cut(s) 951, 1135, 1453
BamHI GGATCC 1 cut(s) 1300
BanI GGYRCC 1 cut(s) 536
Bbv12I GWGCWC 2 cut(s) 11, 824
BbvI GCAGC 2 cut(s) 752, 1531
BccI CCATC 2 cut(s) 680, 865
BciT130I CCWGG 5 cut(s) 110, 174, 249, 348, 594
BciVI GTATCC 1 cut(s) 999
BclI TGATCA 1 cut(s) 148
BcnI CCSGG 1 cut(s) 511
BcoDI GTCTC 1 cut(s) 684
BcuI ACTAGT 1 cut(s) 1061
BfaI CTAG 3 cut(s) 1062, 1076, 1119
BfmI CTRYAG 1 cut(s) 156
BfoI RGCGCY 1 cut(s) 359
BfuAI ACCTGC 1 cut(s) 1468
BfuI GTATCC 1 cut(s) 999
BglII AGATCT 3 cut(s) 904, 1051, 1580
BisI GCNGC 4 cut(s) 452, 741, 1270, 1520
BlsI GCNGC 4 cut(s) 453, 742, 1271, 1521
Bme1390I CCNGG 6 cut(s) 110, 174, 249, 348, 511, 594
Bme18I GGWCC 3 cut(s) 951, 1135, 1453
BmgT120I GGNCC 4 cut(s) 350, 951, 1135, 1453
BmiI GGNNCC 3 cut(s) 538, 1136, 1302
BmrFI CCNGG 6 cut(s) 110, 174, 249, 348, 511, 594
BmrI ACTGGG 2 cut(s) 213, 264
BmsI GCATC 3 cut(s) 752, 1400, 1508
BmuI ACTGGG 2 cut(s) 213, 264
BpmI CTGGAG 2 cut(s) 1316, 1360
Bpu10I CCTNAGC 1 cut(s) 1515
BpuEI CTTGAG 1 cut(s) 1012
BpuMI CCSGG 1 cut(s) 511
Bsa29I ATCGAT 1 cut(s) 734
BsaBI GATNNNNATC 2 cut(s) 624, 1560
BsaJI CCNNGG 3 cut(s) 247, 593, 1129
BsaWI WCCGGW 1 cut(s) 1189
Bsc4I CCNNNNNNNGG 2 cut(s) 254, 450
Bse118I RCCGGY 1 cut(s) 1522
Bse1I ACTGG 2 cut(s) 208, 259
Bse3DI GCAATG 1 cut(s) 1508
Bse8I GATNNNNATC 2 cut(s) 624, 1560
BseBI CCWGG 5 cut(s) 110, 174, 249, 348, 594
BseCI ATCGAT 1 cut(s) 734
BseDI CCNNGG 3 cut(s) 247, 593, 1129
BseGI GGATG 3 cut(s) 118, 151, 653
BseJI GATNNNNATC 2 cut(s) 624, 1560
BseLI CCNNNNNNNGG 2 cut(s) 254, 450
BseMI GCAATG 1 cut(s) 1508
BseNI ACTGG 2 cut(s) 208, 259
BseXI GCAGC 2 cut(s) 752, 1531
BseYI CCCAGC 1 cut(s) 352
BsgI GTGCAG 1 cut(s) 57
Bsh1236I CGCG 2 cut(s) 37, 1000
Bsh1285I CGRYCG 1 cut(s) 41
BshFI GGCC 3 cut(s) 351, 474, 1272
BshNI GGYRCC 1 cut(s) 536
BshVI ATCGAT 1 cut(s) 734
BsiEI CGRYCG 1 cut(s) 41
BsiHKAI GWGCWC 2 cut(s) 11, 824
BsiSI CCGG 3 cut(s) 511, 1190, 1523
BslFI GGGAC 3 cut(s) 272, 493, 1148
BslI CCNNNNNNNGG 2 cut(s) 254, 450
BsmAI GTCTC 1 cut(s) 684
BsmBI CGTCTC 1 cut(s) 684
BsmFI GGGAC 3 cut(s) 272, 493, 1148
BsmI GAATGC 1 cut(s) 928
BsnI GGCC 3 cut(s) 351, 474, 1272
Bsp1286I GDGCHC 2 cut(s) 11, 824
Bsp1407I TGTACA 2 cut(s) 885, 1435
Bsp19I CCATGG 1 cut(s) 1129
BspACI CCGC 3 cut(s) 37, 451, 1269
BspANI GGCC 3 cut(s) 351, 474, 1272
BspDI ATCGAT 1 cut(s) 734
BspFNI CGCG 2 cut(s) 37, 1000
BspHI TCATGA 1 cut(s) 396
BspLI GGNNCC 3 cut(s) 538, 1136, 1302
BspMI ACCTGC 1 cut(s) 1468
BspPI GGATC 5 cut(s) 338, 1253, 1295, 1308, 1556
BspT107I GGYRCC 1 cut(s) 536
BsrBI CCGCTC 1 cut(s) 451
BsrDI GCAATG 1 cut(s) 1508
BsrFI RCCGGY 1 cut(s) 1522
BsrGI TGTACA 2 cut(s) 885, 1435
BsrI ACTGG 2 cut(s) 208, 259
BssAI RCCGGY 1 cut(s) 1522
BssECI CCNNGG 3 cut(s) 247, 593, 1129
BssT1I CCWWGG 1 cut(s) 1129
Bst2UI CCWGG 5 cut(s) 110, 174, 249, 348, 594
Bst4CI ACNGT 7 cut(s) 430, 531, 587, 931, 951, 1283, 1606
BstAUI TGTACA 2 cut(s) 885, 1435
BstC8I GCNNGC 1 cut(s) 1524
BstDEI CTNAG 6 cut(s) 275, 445, 791, 1357, 1406, 1515
BstDSI CCRYGG 1 cut(s) 1129
BstF5I GGATG 3 cut(s) 118, 151, 653
BstFNI CGCG 2 cut(s) 37, 1000
BstH2I RGCGCY 1 cut(s) 359
BstHHI GCGC 4 cut(s) 358, 781, 1000, 1426
BstMAI GTCTC 1 cut(s) 684
BstMCI CGRYCG 1 cut(s) 41
BstMWI GCNNNNNNNGC 3 cut(s) 552, 1469, 1519
BstNI CCWGG 5 cut(s) 110, 174, 249, 348, 594
BstNSI RCATGY 2 cut(s) 1186, 1328
BstSCI CCNGG 6 cut(s) 108, 172, 247, 346, 509, 592
BstSFI CTRYAG 1 cut(s) 156
BstUI CGCG 2 cut(s) 37, 1000
BstV1I GCAGC 2 cut(s) 752, 1531
BstX2I RGATCY 4 cut(s) 904, 1051, 1300, 1580
BstYI RGATCY 4 cut(s) 904, 1051, 1300, 1580
Bsu15I ATCGAT 1 cut(s) 734
BsuI GTATCC 1 cut(s) 999
BsuRI GGCC 3 cut(s) 351, 474, 1272
BsuTUI ATCGAT 1 cut(s) 734
BtgI CCRYGG 1 cut(s) 1129
BtsCI GGATG 3 cut(s) 118, 151, 653
BtsI GCAGTG 1 cut(s) 807
BtsIMutI CAGTG 7 cut(s) 435, 536, 807, 822, 1288, 1376, 1551
BveI ACCTGC 1 cut(s) 1468
Cac8I GCNNGC 1 cut(s) 1524
CaiI CAGNNNCTG 1 cut(s) 434
CciI TCATGA 1 cut(s) 396
CfoI GCGC 4 cut(s) 358, 781, 1000, 1426
Cfr10I RCCGGY 1 cut(s) 1522
Cfr13I GGNCC 4 cut(s) 350, 951, 1135, 1453
ClaI ATCGAT 1 cut(s) 734
Csp6I GTAC 4 cut(s) 523, 886, 1195, 1436
CviQI GTAC 4 cut(s) 523, 886, 1195, 1436
DdeI CTNAG 6 cut(s) 275, 445, 791, 1357, 1406, 1515
EaeI YGGCCR 1 cut(s) 1270
Eco130I CCWWGG 1 cut(s) 1129
Eco47I GGWCC 3 cut(s) 951, 1135, 1453
Eco57I CTGAAG 1 cut(s) 1400
EcoRI GAATTC 1 cut(s) 491
EcoRII CCWGG 5 cut(s) 108, 172, 247, 346, 592
EcoT14I CCWWGG 1 cut(s) 1129
EcoT22I ATGCAT 2 cut(s) 295, 1415
ErhI CCWWGG 1 cut(s) 1129
Esp3I CGTCTC 1 cut(s) 684
FaqI GGGAC 3 cut(s) 272, 493, 1148
FauNDI CATATG 1 cut(s) 1174
FbaI TGATCA 1 cut(s) 148
FblI GTMKAC 1 cut(s) 23
Fnu4HI GCNGC 4 cut(s) 452, 741, 1270, 1520
FokI GGATG 3 cut(s) 125, 138, 660
Fsp4HI GCNGC 4 cut(s) 452, 741, 1270, 1520
FspBI CTAG 3 cut(s) 1062, 1076, 1119
FspI TGCGCA 1 cut(s) 780
GlaI GCGC 4 cut(s) 357, 780, 999, 1425
GluI GCNGC 4 cut(s) 452, 741, 1270, 1520
GsaI CCCAGC 1 cut(s) 356
GsuI CTGGAG 2 cut(s) 1316, 1360
HaeII RGCGCY 1 cut(s) 359
HaeIII GGCC 3 cut(s) 351, 474, 1272
HapII CCGG 3 cut(s) 511, 1190, 1523
HhaI GCGC 4 cut(s) 358, 781, 1000, 1426
Hin6I GCGC 4 cut(s) 356, 779, 998, 1424
HinP1I GCGC 4 cut(s) 356, 779, 998, 1424
HincII GTYRAC 1 cut(s) 55
HindII GTYRAC 1 cut(s) 55
HinfI GANTC 5 cut(s) 437, 625, 1025, 1370, 1445
HpaI GTTAAC 1 cut(s) 55
HpaII CCGG 3 cut(s) 511, 1190, 1523
HphI GGTGA 4 cut(s) 202, 1010, 1179, 1505
Hpy166II GTNNAC 6 cut(s) 24, 55, 886, 888, 1436, 1534
Hpy188I TCNGA 7 cut(s) 283, 630, 1056, 1105, 1169, 1228, 1291
Hpy188III TCNNGA 6 cut(s) 377, 397, 725, 1029, 1295, 1339
Hpy8I GTNNAC 6 cut(s) 24, 55, 886, 888, 1436, 1534
HpyAV CCTTC 7 cut(s) 478, 485, 827, 964, 1314, 1340, 1515
HpyCH4III ACNGT 7 cut(s) 430, 531, 587, 931, 951, 1283, 1606
HpyCH4IV ACGT 1 cut(s) 912
HpyCH4V TGCA 9 cut(s) 74, 293, 413, 500, 807, 812, 926, 1144, 1413
HpyF10VI GCNNNNNNNGC 3 cut(s) 552, 1469, 1519
HpyF3I CTNAG 6 cut(s) 275, 445, 791, 1357, 1406, 1515
HpySE526I ACGT 1 cut(s) 912
HspAI GCGC 4 cut(s) 356, 779, 998, 1424
KroI GCCGGC 1 cut(s) 1522
KroNI GCCGGC 1 cut(s) 1524
Ksp22I TGATCA 1 cut(s) 148
KspAI GTTAAC 1 cut(s) 55
LmnI GCTCC 1 cut(s) 1341
Lsp1109I GCAGC 2 cut(s) 752, 1531
LweI GCATC 3 cut(s) 752, 1400, 1508
MaeI CTAG 3 cut(s) 1062, 1076, 1119
MaeII ACGT 1 cut(s) 912
MaeIII GTNAC 7 cut(s) 234, 367, 430, 515, 581, 1185, 1606
MbiI CCGCTC 1 cut(s) 451
MboII GAAGA 6 cut(s) 500, 1007, 1454, 1551, 1575, 1578
MflI RGATCY 4 cut(s) 904, 1051, 1300, 1580
MhlI GDGCHC 2 cut(s) 11, 824
MlyI GAGTC 1 cut(s) 1019
MmeI TCCRAC 2 cut(s) 1147, 1236
MnlI CCTC 1 cut(s) 1291
Mph1103I ATGCAT 2 cut(s) 295, 1415
MroNI GCCGGC 1 cut(s) 1522
MroXI GAANNNNTTC 1 cut(s) 799
MseI TTAA 8 cut(s) 54, 167, 221, 567, 717, 749, 1034, 1316
MslI CAYNNNNRTG 1 cut(s) 881
MspI CCGG 3 cut(s) 511, 1190, 1523
MspR9I CCNGG 6 cut(s) 110, 174, 249, 348, 511, 594
Mva1269I GAATGC 1 cut(s) 928
MvaI CCWGG 5 cut(s) 110, 174, 249, 348, 594
MvnI CGCG 2 cut(s) 37, 1000
MwoI GCNNNNNNNGC 3 cut(s) 552, 1469, 1519
NaeI GCCGGC 1 cut(s) 1524
NciI CCSGG 1 cut(s) 511
NcoI CCATGG 1 cut(s) 1129
NdeI CATATG 1 cut(s) 1174
NgoMIV GCCGGC 1 cut(s) 1522
NlaIV GGNNCC 3 cut(s) 538, 1136, 1302
NmuCI GTSAC 6 cut(s) 234, 367, 430, 515, 581, 1185
NsbI TGCGCA 1 cut(s) 780
NsiI ATGCAT 2 cut(s) 295, 1415
NspI RCATGY 2 cut(s) 1186, 1328
PagI TCATGA 1 cut(s) 396
PciI ACATGT 2 cut(s) 1182, 1324
PctI GAATGC 1 cut(s) 928
PdiI GCCGGC 1 cut(s) 1524
PdmI GAANNNNTTC 1 cut(s) 799
PfeI GAWTC 4 cut(s) 437, 625, 1370, 1445
PfoI TCCNGGA 1 cut(s) 108
PkrI GCNGC 4 cut(s) 453, 742, 1271, 1521
PleI GAGTC 1 cut(s) 1019
PpsI GAGTC 1 cut(s) 1019
PscI ACATGT 2 cut(s) 1182, 1324
PsiI TTATAA 1 cut(s) 701
Psp6I CCWGG 5 cut(s) 108, 172, 247, 346, 592
PspFI CCCAGC 1 cut(s) 352
PspGI CCWGG 5 cut(s) 108, 172, 247, 346, 592
PspN4I GGNNCC 3 cut(s) 538, 1136, 1302
PspPI GGNCC 4 cut(s) 350, 951, 1135, 1453
PstNI CAGNNNCTG 1 cut(s) 434
PsuI RGATCY 4 cut(s) 904, 1051, 1300, 1580
RsaI GTAC 4 cut(s) 524, 887, 1196, 1437
RsaNI GTAC 4 cut(s) 523, 886, 1195, 1436
RseI CAYNNNNRTG 1 cut(s) 881
SaqAI TTAA 8 cut(s) 54, 167, 221, 567, 717, 749, 1034, 1316
SatI GCNGC 4 cut(s) 452, 741, 1270, 1520
Sau96I GGNCC 4 cut(s) 350, 951, 1135, 1453
SchI GAGTC 1 cut(s) 1019
ScrFI CCNGG 6 cut(s) 110, 174, 249, 348, 511, 594
SduI GDGCHC 2 cut(s) 11, 824
SfaNI GCATC 3 cut(s) 752, 1400, 1508
SfcI CTRYAG 1 cut(s) 156
SinI GGWCC 3 cut(s) 951, 1135, 1453
SmiMI CAYNNNNRTG 1 cut(s) 881
SmlI CTYRAG 1 cut(s) 1027
SmoI CTYRAG 1 cut(s) 1027
SpeI ACTAGT 1 cut(s) 1061
SsiI CCGC 3 cut(s) 37, 451, 1269
SspI AATATT 1 cut(s) 1125
SspMI CTAG 3 cut(s) 1062, 1076, 1119
StyD4I CCNGG 6 cut(s) 108, 172, 247, 346, 509, 592
StyI CCWWGG 1 cut(s) 1129
TaaI ACNGT 7 cut(s) 430, 531, 587, 931, 951, 1283, 1606
TaiI ACGT 1 cut(s) 915
TaqI TCGA 3 cut(s) 661, 734, 1624
TaqII GACCGA 1 cut(s) 1470
TatI WGTACW 2 cut(s) 885, 1435
TauI GCSGC 2 cut(s) 454, 1272
TfiI GAWTC 4 cut(s) 437, 625, 1370, 1445
Tru1I TTAA 8 cut(s) 54, 167, 221, 567, 717, 749, 1034, 1316
Tru9I TTAA 8 cut(s) 54, 167, 221, 567, 717, 749, 1034, 1316
TscAI CASTG 7 cut(s) 435, 536, 814, 829, 1288, 1383, 1558
TseFI GTSAC 6 cut(s) 234, 367, 430, 515, 581, 1185
TseI GCWGC 2 cut(s) 740, 1519
Tsp45I GTSAC 6 cut(s) 234, 367, 430, 515, 581, 1185
TspGWI ACGGA 1 cut(s) 540
TspRI CASTG 7 cut(s) 435, 536, 814, 829, 1288, 1383, 1558
VpaK11BI GGWCC 3 cut(s) 951, 1135, 1453
XapI RAATTY 4 cut(s) 94, 491, 1098, 1543
XceI RCATGY 2 cut(s) 1186, 1328
XcmI CCANNNNNNNNNTGG 1 cut(s) 1280
XmiI GTMKAC 1 cut(s) 23
XmnI GAANNNNTTC 1 cut(s) 799
XspI CTAG 3 cut(s) 1062, 1076, 1119
Zsp2I ATGCAT 2 cut(s) 295, 1415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.