Rmu_sc0011169.1_g000007

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0011169.1
OG0000112
Physical Location & Seq
Reverse (-)
13195 .. 16410
3216 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0011169.1_g000007.1.cds

Sequence Viewer

Length: 2487 bp
atgagaaacaagatgccattggttcattcctacagaattgaaaatgcagagaaaatggttttgaaagctctgcttctcttcctcctctttcagttctgcacttccagggacaccatgagagggaacggagaacaattgaaggatgttgatggtgactatttagtgtccaaagaaagcaagtttgaattgggtttcttcagccctggaaattctagctaccggtatgttggaatttggtattctcagactagagtatctgaaaaaacagtggtgtgggttgcaaacagaaacaatcccatcaatgatacctctggtgtgctcacatttaacaggtatggacaactagtcctttatgctcataacatggagagtattcctatatggtctacacacgtgtcgcagtcggtccaaaatgttaacacaagcactttatctgcacagcttttggatacagcaaatttagttgtgttccaggatgataataatgatatctttaaatggcaaagttttgattatcctacagatactctgcttccaggaatgagacttggattgaatcggaaaactgggctagaatgggctttaacatcctggaagtcacaagatgaccctggaactggggactatacacataggctttatttgaatcagactgcaaccccccaattttttacatataaaggttcaagtaagtattggcgaagtgatccagggccatattctacgttggtcactaatcaagacgaaacttattttttcctgaataacaccaatggaattgcaagaataattttgaatggttcagagttacagtgccttacatggagtgatggagaccatcaatggaaggaaacctatgctgtaccgacattccggtgtgactggtatggacattgtggtgccaacagcaaatgtaaccctgacaatattagtctgtttgagtgtgactgtctacccggttttgaacctaaatctgtcaatgagtggaatcagaataatgggtcagatggatgtgtgagtaagcgaaatgaagtttctaagtgtgagaatgaagaagggtttgtgaaggtcacaagagttaaaattccagacgtatcaatagcagctcgattggaagcacgtatgagtaacaaagtgtgtaggcagaagtgcttggcaaattgttcttgcacagcatatatgggcattgaaaatgaaaaaggggttgctgattgtttgacatggtatgatgatttgatggatattttagtgtacgcagaggttggacgagatctatatgttcgtgtggatcgaatcgaattagctgagaattctagaaaatcaaaaggtttctcggaaagaaggggtatggtggctattccaatactcttttctctgctagcactggtactaatcattatgttcgcttgctggtggcgtaagaaaaaaaggaatacaacagaaattttggaggcggatgaacttgaggaaactaagagacaccccgaattgcaattttttgatatgaacacactaatggcagccacagataacttctctcatgtcaatgaactcggccaaggtggttttggcgctgtttataagggtcagttaccaaatgagcagaaggtagctgtaaaaaggttgtctaaaacatcaggacaagggactgaagaattcaaaaatgaagttgcacttgtagcaagacttcagcaccgcaatctcgtaaaactcttaggctgttgtataaagggggaagaacgaatgttagtcctggaatacatgccgaacaaaagcttggactcatttctctttgatcacacaagacggtcctttttggattgggaaaagcgctttgaaattatcaacgggattgctcgtgggattctgtatcttcaccaagactcaagattgaggattatccatagagatctaaaaaccagtaatgttcttctagatgctgaaatgaacccaaaaatttctgattttggcatggcaagaatatttgacggggaccaaattcaggataaaacaaaccgagttgttggaacatatggctatatgtcaccagagtatgcagtatttggcagattttctactaaatctgatgtctttagttttgggatcattttgttagaggttgtaactggcaaaagaaacaacggtttttctcaagaggatccttccatgaacttgataagacacgtttgggaactctggagagaagatagagccttagaacttgtggattcgaatctagagtcatataattctgatgaggtcatgagatgcattcaacttgcgctcttgtgcgtgcaagaagaatcaaaggacaggccagccatgtctgccattgttttcatgttgagtggtgaaacatctcctccatcacctaaccaacctgcatatgttttcagaagaacctccagcactaatgctgatccatcagtaccaaaacgattatattctattaacgacttgacaataactgcagaggaaggtcggtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

828

Amino Acids

94.64

Weight (kDa)

5.94

Isoelectric Point (pI)

39.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1581
Acc36I ACCTGC 1 cut(s) 2389
AccB1I GGYRCC 1 cut(s) 899
AccI GTMKAC 2 cut(s) 386, 952
AciI CCGC 2 cut(s) 1454, 1696
AclWI GGATC 6 cut(s) 701, 1296, 2111, 2153, 2166, 2414
AcoI YGGCCR 1 cut(s) 1555
AcsI RAATTY 9 cut(s) 208, 231, 457, 1083, 1309, 1443, 1655, 1956, 1998
AcuI CTGAAG 3 cut(s) 181, 1671, 1673
AcvI CACGTG 1 cut(s) 394
AfaI GTAC 4 cut(s) 864, 1253, 1389, 2430
AfeI AGCGCT 1 cut(s) 1832
AfiI CCNNNNNNNGG 3 cut(s) 120, 617, 2002
AflIII ACRYGT 3 cut(s) 391, 393, 2182
AgeI ACCGGT 1 cut(s) 219
AhdI GACNNNNNGTC 1 cut(s) 344
AhlI ACTAGT 1 cut(s) 343
AjnI CCWGG 8 cut(s) 104, 202, 471, 535, 590, 610, 709, 1752
AjuI GAANNNNNNNTTGG 4 cut(s) 1354, 1386, 1760, 1792
AluBI AGCT 7 cut(s) 68, 216, 442, 1106, 1304, 1613, 1776
AluI AGCT 7 cut(s) 68, 216, 442, 1106, 1304, 1613, 1776
Alw21I GWGCWC 1 cut(s) 321
Alw26I GTCTC 3 cut(s) 538, 828, 1471
AlwI GGATC 6 cut(s) 701, 1296, 2111, 2153, 2166, 2414
Aor51HI AGCGCT 1 cut(s) 1832
AoxI GGCC 3 cut(s) 713, 1555, 2315
ApeKI GCWGC 2 cut(s) 1103, 1520
ApoI RAATTY 9 cut(s) 208, 231, 457, 1083, 1309, 1443, 1655, 1956, 1998
ArsI GACNNNNNNTTYG 2 cut(s) 2225, 2257
AsiGI ACCGGT 1 cut(s) 219
AspLEI GCGC 3 cut(s) 1574, 1833, 2284
AspS9I GGNCC 4 cut(s) 406, 713, 1809, 1993
AsuC2I CCSGG 1 cut(s) 957
AsuHPI GGTGA 5 cut(s) 164, 1868, 2037, 2361, 2363
AsuII TTCGAA 1 cut(s) 2231
AsuNHI GCTAGC 1 cut(s) 1378
AvaII GGWCC 3 cut(s) 406, 1809, 1993
BaeI ACNNNNGTAYC 2 cut(s) 237, 270
BamHI GGATCC 1 cut(s) 2158
BanI GGYRCC 1 cut(s) 899
BarI GAAGNNNNNNTAC 2 cut(s) 1671, 1703
BauI CACGAG 1 cut(s) 1857
BbrPI CACGTG 1 cut(s) 394
Bbv12I GWGCWC 1 cut(s) 321
BbvI GCAGC 2 cut(s) 1115, 1532
BccI CCATC 8 cut(s) 143, 305, 824, 846, 1001, 1231, 2374, 2431
BciT130I CCWGG 8 cut(s) 106, 204, 473, 537, 592, 612, 711, 1754
BciVI GTATCC 1 cut(s) 442
BclI TGATCA 1 cut(s) 1795
BcnI CCSGG 1 cut(s) 957
BcoDI GTCTC 3 cut(s) 538, 828, 1471
BcuI ACTAGT 1 cut(s) 343
BfaI CTAG 8 cut(s) 213, 249, 344, 572, 1314, 1379, 1934, 2237
BfmI CTRYAG 3 cut(s) 31, 519, 2469
BfoI RGCGCY 2 cut(s) 1575, 1834
BfuAI ACCTGC 1 cut(s) 2389
BfuI GTATCC 1 cut(s) 442
BglII AGATCT 2 cut(s) 1270, 1909
BisI GCNGC 2 cut(s) 1104, 1521
BlsI GCNGC 2 cut(s) 1105, 1522
Bme1390I CCNGG 9 cut(s) 106, 204, 473, 537, 592, 612, 711, 957, 1754
Bme18I GGWCC 3 cut(s) 406, 1809, 1993
BmeRI GACNNNNNGTC 1 cut(s) 344
BmgT120I GGNCC 4 cut(s) 406, 713, 1809, 1993
BmiI GGNNCC 3 cut(s) 901, 1994, 2160
BmrFI CCNGG 9 cut(s) 106, 204, 473, 537, 592, 612, 711, 957, 1754
BmrI ACTGGG 2 cut(s) 576, 627
BmsI GCATC 3 cut(s) 3, 1927, 2258
BmtI GCTAGC 1 cut(s) 1382
BmuI ACTGGG 2 cut(s) 576, 627
BpmI CTGGAG 2 cut(s) 2218, 2389
Bpu14I TTCGAA 1 cut(s) 2231
BpuEI CTTGAG 3 cut(s) 1484, 1870, 2136
BpuMI CCSGG 1 cut(s) 957
BsaAI YACGTR 2 cut(s) 394, 1121
BsaBI GATNNNNATC 1 cut(s) 2232
BsaI GGTCTC 1 cut(s) 828
BsaJI CCNNGG 5 cut(s) 105, 202, 610, 710, 1558
BsaWI WCCGGW 2 cut(s) 219, 873
BsaXI ACNNNNNCTCC 2 cut(s) 2347, 2377
Bsc4I CCNNNNNNNGG 3 cut(s) 120, 617, 2002
Bse118I RCCGGY 1 cut(s) 219
Bse1I ACTGG 6 cut(s) 571, 622, 887, 1389, 1920, 2131
Bse8I GATNNNNATC 1 cut(s) 2232
BseBI CCWGG 8 cut(s) 106, 204, 473, 537, 592, 612, 711, 1754
BseDI CCNNGG 5 cut(s) 105, 202, 610, 710, 1558
BseGI GGATG 5 cut(s) 148, 481, 587, 1016, 1462
BseJI GATNNNNATC 1 cut(s) 2232
BseLI CCNNNNNNNGG 3 cut(s) 120, 617, 2002
BseMII CTCAG 2 cut(s) 257, 1296
BseNI ACTGG 6 cut(s) 571, 622, 887, 1389, 1920, 2131
BseRI GAGGAG 2 cut(s) 74, 2352
BseXI GCAGC 2 cut(s) 1115, 1532
BsgI GTGCAG 2 cut(s) 82, 420
BshFI GGCC 3 cut(s) 715, 1557, 2317
BshNI GGYRCC 1 cut(s) 899
BshTI ACCGGT 1 cut(s) 219
BsiHKAI GWGCWC 1 cut(s) 321
BsiSI CCGG 3 cut(s) 220, 874, 957
BslFI GGGAC 4 cut(s) 122, 635, 1660, 2006
BslI CCNNNNNNNGG 3 cut(s) 120, 617, 2002
BsmAI GTCTC 3 cut(s) 538, 828, 1471
BsmFI GGGAC 4 cut(s) 122, 635, 1660, 2006
BsmI GAATGC 1 cut(s) 2271
BsnI GGCC 3 cut(s) 715, 1557, 2317
Bso31I GGTCTC 1 cut(s) 828
Bsp119I TTCGAA 1 cut(s) 2231
Bsp1286I GDGCHC 1 cut(s) 321
Bsp143I GATC 8 cut(s) 706, 1270, 1288, 1795, 1909, 2103, 2158, 2419
BspACI CCGC 2 cut(s) 1454, 1696
BspANI GGCC 3 cut(s) 715, 1557, 2317
BspCNI CTCAG 2 cut(s) 256, 1297
BspHI TCATGA 1 cut(s) 2262
BspLI GGNNCC 3 cut(s) 901, 1994, 2160
BspMAI CTGCAG 1 cut(s) 2473
BspMI ACCTGC 1 cut(s) 2389
BspOI GCTAGC 1 cut(s) 1382
BspPI GGATC 6 cut(s) 701, 1296, 2111, 2153, 2166, 2414
BspT104I TTCGAA 1 cut(s) 2231
BspT107I GGYRCC 1 cut(s) 899
BspTNI GGTCTC 1 cut(s) 828
BsrFI RCCGGY 1 cut(s) 219
BsrI ACTGG 6 cut(s) 571, 622, 887, 1389, 1920, 2131
BssAI RCCGGY 1 cut(s) 219
BssECI CCNNGG 5 cut(s) 105, 202, 610, 710, 1558
BssMI GATC 8 cut(s) 706, 1270, 1288, 1795, 1909, 2103, 2158, 2419
BssSI CACGAG 1 cut(s) 1857
BssT1I CCWWGG 1 cut(s) 1558
Bst2BI CACGAG 1 cut(s) 1857
Bst2UI CCWGG 8 cut(s) 106, 204, 473, 537, 592, 612, 711, 1754
Bst4CI ACNGT 5 cut(s) 268, 813, 950, 1809, 2144
Bst6I CTCTTC 1 cut(s) 83
BstBAI YACGTR 2 cut(s) 394, 1121
BstBI TTCGAA 1 cut(s) 2231
BstC8I GCNNGC 4 cut(s) 1380, 1408, 2294, 2319
BstDEI CTNAG 6 cut(s) 243, 1038, 1305, 1473, 1714, 2215
BstF5I GGATG 5 cut(s) 148, 481, 587, 1016, 1462
BstH2I RGCGCY 2 cut(s) 1575, 1834
BstHHI GCGC 3 cut(s) 1574, 1833, 2284
BstKTI GATC 8 cut(s) 709, 1273, 1291, 1798, 1912, 2106, 2161, 2422
BstMAI GTCTC 3 cut(s) 538, 828, 1471
BstMBI GATC 8 cut(s) 706, 1270, 1288, 1795, 1909, 2103, 2158, 2419
BstMWI GCNNNNNNNGC 2 cut(s) 1679, 2327
BstNI CCWGG 8 cut(s) 106, 204, 473, 537, 592, 612, 711, 1754
BstNSI RCATGY 1 cut(s) 1765
BstSCI CCNGG 9 cut(s) 104, 202, 471, 535, 590, 610, 709, 955, 1752
BstSFI CTRYAG 3 cut(s) 31, 519, 2469
BstV1I GCAGC 2 cut(s) 1115, 1532
BstX2I RGATCY 3 cut(s) 1270, 1909, 2158
BstYI RGATCY 3 cut(s) 1270, 1909, 2158
BsuI GTATCC 1 cut(s) 442
BsuRI GGCC 3 cut(s) 715, 1557, 2317
BtsCI GGATG 5 cut(s) 148, 481, 587, 1016, 1462
BtsIMutI CAGTG 3 cut(s) 273, 818, 1382
BveI ACCTGC 1 cut(s) 2389
Cac8I GCNNGC 4 cut(s) 1380, 1408, 2294, 2319
CciI TCATGA 1 cut(s) 2262
CfoI GCGC 3 cut(s) 1574, 1833, 2284
Cfr10I RCCGGY 1 cut(s) 219
Cfr13I GGNCC 4 cut(s) 406, 713, 1809, 1993
Csp6I GTAC 4 cut(s) 863, 1252, 1388, 2429
CspAI ACCGGT 1 cut(s) 219
CviQI GTAC 4 cut(s) 863, 1252, 1388, 2429
DdeI CTNAG 6 cut(s) 243, 1038, 1305, 1473, 1714, 2215
DpnI GATC 8 cut(s) 708, 1272, 1290, 1797, 1911, 2105, 2160, 2421
DpnII GATC 8 cut(s) 706, 1270, 1288, 1795, 1909, 2103, 2158, 2419
DraI TTTAAA 1 cut(s) 496
DriI GACNNNNNGTC 1 cut(s) 344
EaeI YGGCCR 1 cut(s) 1555
Eam1104I CTCTTC 1 cut(s) 83
Eam1105I GACNNNNNGTC 1 cut(s) 344
EarI CTCTTC 1 cut(s) 83
EciI GGCGGA 1 cut(s) 1469
Eco130I CCWWGG 1 cut(s) 1558
Eco31I GGTCTC 1 cut(s) 828
Eco32I GATATC 1 cut(s) 490
Eco47I GGWCC 3 cut(s) 406, 1809, 1993
Eco47III AGCGCT 1 cut(s) 1832
Eco57I CTGAAG 3 cut(s) 181, 1671, 1673
Eco72I CACGTG 1 cut(s) 394
EcoRI GAATTC 2 cut(s) 1309, 1655
EcoRII CCWGG 8 cut(s) 104, 202, 471, 535, 590, 610, 709, 1752
EcoRV GATATC 1 cut(s) 490
EcoT14I CCWWGG 1 cut(s) 1558
EcoT22I ATGCAT 1 cut(s) 2273
ErhI CCWWGG 1 cut(s) 1558
FalI AAGNNNNNCTT 4 cut(s) 57, 89, 1659, 1691
FaqI GGGAC 4 cut(s) 122, 635, 1660, 2006
FauNDI CATATG 2 cut(s) 2032, 2386
FbaI TGATCA 1 cut(s) 1795
FblI GTMKAC 2 cut(s) 386, 952
Fnu4HI GCNGC 2 cut(s) 1104, 1521
FokI GGATG 5 cut(s) 155, 488, 574, 1023, 1469
Fsp4HI GCNGC 2 cut(s) 1104, 1521
FspBI CTAG 8 cut(s) 213, 249, 344, 572, 1314, 1379, 1934, 2237
GlaI GCGC 3 cut(s) 1573, 1832, 2283
GluI GCNGC 2 cut(s) 1104, 1521
GsuI CTGGAG 2 cut(s) 2218, 2389
HaeII RGCGCY 2 cut(s) 1575, 1834
HaeIII GGCC 3 cut(s) 715, 1557, 2317
HapII CCGG 3 cut(s) 220, 874, 957
HhaI GCGC 3 cut(s) 1574, 1833, 2284
Hin6I GCGC 3 cut(s) 1572, 1831, 2282
HinP1I GCGC 3 cut(s) 1572, 1831, 2282
HincII GTYRAC 1 cut(s) 418
HindII GTYRAC 1 cut(s) 418
HindIII AAGCTT 1 cut(s) 1774
HpaI GTTAAC 1 cut(s) 418
HpaII CCGG 3 cut(s) 220, 874, 957
HphI GGTGA 5 cut(s) 164, 1868, 2037, 2361, 2363
Hpy166II GTNNAC 4 cut(s) 387, 418, 953, 1252
Hpy8I GTNNAC 4 cut(s) 387, 418, 953, 1252
HpyAV CCTTC 8 cut(s) 133, 841, 1049, 1060, 1335, 1600, 2172, 2471
HpyCH4III ACNGT 5 cut(s) 268, 813, 950, 1809, 2144
HpyCH4IV ACGT 5 cut(s) 393, 725, 1092, 1120, 2184
HpyF10VI GCNNNNNNNGC 2 cut(s) 1679, 2327
HpyF3I CTNAG 6 cut(s) 243, 1038, 1305, 1473, 1714, 2215
HpySE526I ACGT 5 cut(s) 393, 725, 1092, 1120, 2184
HspAI GCGC 3 cut(s) 1572, 1831, 2282
Ksp22I TGATCA 1 cut(s) 1795
KspAI GTTAAC 1 cut(s) 418
Kzo9I GATC 8 cut(s) 706, 1270, 1288, 1795, 1909, 2103, 2158, 2419
Lsp1109I GCAGC 2 cut(s) 1115, 1532
LweI GCATC 3 cut(s) 3, 1927, 2258
MaeI CTAG 8 cut(s) 213, 249, 344, 572, 1314, 1379, 1934, 2237
MaeII ACGT 5 cut(s) 393, 725, 1092, 1120, 2184
MalI GATC 8 cut(s) 708, 1272, 1290, 1797, 1911, 2105, 2160, 2421
MboI GATC 8 cut(s) 706, 1270, 1288, 1795, 1909, 2103, 2158, 2419
MfeI CAATTG 1 cut(s) 134
MflI RGATCY 3 cut(s) 1270, 1909, 2158
MhlI GDGCHC 1 cut(s) 321
MlyI GAGTC 3 cut(s) 1775, 1877, 2249
MmeI TCCRAC 3 cut(s) 208, 1243, 2005
Mph1103I ATGCAT 1 cut(s) 2273
MseI TTAA 6 cut(s) 327, 417, 495, 584, 1080, 2451
MslI CAYNNNNRTG 4 cut(s) 874, 897, 1514, 1545
MspI CCGG 3 cut(s) 220, 874, 957
MspR9I CCNGG 9 cut(s) 106, 204, 473, 537, 592, 612, 711, 957, 1754
MunI CAATTG 1 cut(s) 134
Mva1269I GAATGC 1 cut(s) 2271
MvaI CCWGG 8 cut(s) 106, 204, 473, 537, 592, 612, 711, 1754
MwoI GCNNNNNNNGC 2 cut(s) 1679, 2327
NciI CCSGG 1 cut(s) 957
NdeI CATATG 2 cut(s) 2032, 2386
NdeII GATC 8 cut(s) 706, 1270, 1288, 1795, 1909, 2103, 2158, 2419
NheI GCTAGC 1 cut(s) 1378
NlaIV GGNNCC 3 cut(s) 901, 1994, 2160
NmeAIII GCCGAG 1 cut(s) 1533
NmuCI GTSAC 7 cut(s) 152, 597, 730, 878, 944, 1069, 2043
NsiI ATGCAT 1 cut(s) 2273
NspI RCATGY 1 cut(s) 1765
NspV TTCGAA 1 cut(s) 2231
PagI TCATGA 1 cut(s) 2262
PcsI WCGNNNNNNNCGW 1 cut(s) 1288
PctI GAATGC 1 cut(s) 2271
PfeI GAWTC 8 cut(s) 556, 646, 988, 1293, 1864, 2228, 2233, 2303
PfoI TCCNGGA 4 cut(s) 471, 535, 590, 1752
PinAI ACCGGT 1 cut(s) 219
PkrI GCNGC 2 cut(s) 1105, 1522
PleI GAGTC 3 cut(s) 1775, 1877, 2248
PmaCI CACGTG 1 cut(s) 394
PmlI CACGTG 1 cut(s) 394
PpsI GAGTC 3 cut(s) 1775, 1877, 2248
Ppu21I YACGTR 2 cut(s) 394, 1121
PsiI TTATAA 1 cut(s) 1581
Psp6I CCWGG 8 cut(s) 104, 202, 471, 535, 590, 610, 709, 1752
PspCI CACGTG 1 cut(s) 394
PspGI CCWGG 8 cut(s) 104, 202, 471, 535, 590, 610, 709, 1752
PspN4I GGNNCC 3 cut(s) 901, 1994, 2160
PspPI GGNCC 4 cut(s) 406, 713, 1809, 1993
PstI CTGCAG 1 cut(s) 2473
PsuI RGATCY 3 cut(s) 1270, 1909, 2158
RsaI GTAC 4 cut(s) 864, 1253, 1389, 2430
RsaNI GTAC 4 cut(s) 863, 1252, 1388, 2429
RseI CAYNNNNRTG 4 cut(s) 874, 897, 1514, 1545
SaqAI TTAA 6 cut(s) 327, 417, 495, 584, 1080, 2451
SatI GCNGC 2 cut(s) 1104, 1521
Sau3AI GATC 8 cut(s) 706, 1270, 1288, 1795, 1909, 2103, 2158, 2419
Sau96I GGNCC 4 cut(s) 406, 713, 1809, 1993
SchI GAGTC 3 cut(s) 1775, 1877, 2249
ScrFI CCNGG 9 cut(s) 106, 204, 473, 537, 592, 612, 711, 957, 1754
SduI GDGCHC 1 cut(s) 321
SfaNI GCATC 3 cut(s) 3, 1927, 2258
SfcI CTRYAG 3 cut(s) 31, 519, 2469
SfuI TTCGAA 1 cut(s) 2231
SinI GGWCC 3 cut(s) 406, 1809, 1993
SmiMI CAYNNNNRTG 4 cut(s) 874, 897, 1514, 1545
SmlI CTYRAG 3 cut(s) 1463, 1885, 2151
SmoI CTYRAG 3 cut(s) 1463, 1885, 2151
SpeI ACTAGT 1 cut(s) 343
SsiI CCGC 2 cut(s) 1454, 1696
SspI AATATT 2 cut(s) 928, 1983
SspMI CTAG 8 cut(s) 213, 249, 344, 572, 1314, 1379, 1934, 2237
StyD4I CCNGG 9 cut(s) 104, 202, 471, 535, 590, 610, 709, 955, 1752
StyI CCWWGG 1 cut(s) 1558
TaaI ACNGT 5 cut(s) 268, 813, 950, 1809, 2144
TaiI ACGT 5 cut(s) 396, 728, 1095, 1123, 2187
TaqI TCGA 4 cut(s) 1108, 1291, 1296, 2231
TaqII GACCGA 1 cut(s) 394
TfiI GAWTC 8 cut(s) 556, 646, 988, 1293, 1864, 2228, 2233, 2303
Tru1I TTAA 6 cut(s) 327, 417, 495, 584, 1080, 2451
Tru9I TTAA 6 cut(s) 327, 417, 495, 584, 1080, 2451
TscAI CASTG 3 cut(s) 273, 818, 1389
TseFI GTSAC 7 cut(s) 152, 597, 730, 878, 944, 1069, 2043
TseI GCWGC 2 cut(s) 1103, 1520
Tsp45I GTSAC 7 cut(s) 152, 597, 730, 878, 944, 1069, 2043
TspGWI ACGGA 1 cut(s) 141
TspRI CASTG 3 cut(s) 273, 818, 1389
VpaK11BI GGWCC 3 cut(s) 406, 1809, 1993
XapI RAATTY 9 cut(s) 208, 231, 457, 1083, 1309, 1443, 1655, 1956, 1998
XbaI TCTAGA 3 cut(s) 1313, 1933, 2236
XceI RCATGY 1 cut(s) 1765
XcmI CCANNNNNNNNNTGG 1 cut(s) 1565
XmiI GTMKAC 2 cut(s) 386, 952
XspI CTAG 8 cut(s) 213, 249, 344, 572, 1314, 1379, 1934, 2237
Zsp2I ATGCAT 1 cut(s) 2273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.