serine threonine-protein kinase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 363 |
| AccB1I | GGYRCC | 1 cut(s) | 328 |
| AccI | GTMKAC | 1 cut(s) | 747 |
| AccII | CGCG | 1 cut(s) | 744 |
| AciI | CCGC | 1 cut(s) | 664 |
| AclWI | GGATC | 2 cut(s) | 47, 542 |
| AfaI | GTAC | 1 cut(s) | 137 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 77, 177 |
| AgsI | TTSAA | 2 cut(s) | 16, 75 |
| AjnI | CCWGG | 1 cut(s) | 418 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 14, 46 |
| AluBI | AGCT | 1 cut(s) | 149 |
| AluI | AGCT | 1 cut(s) | 149 |
| AlwI | GGATC | 2 cut(s) | 47, 542 |
| Ama87I | CYCGRG | 1 cut(s) | 170 |
| AoxI | GGCC | 2 cut(s) | 173, 179 |
| ApeKI | GCWGC | 1 cut(s) | 566 |
| AspS9I | GGNCC | 2 cut(s) | 173, 365 |
| AsuC2I | CCSGG | 3 cut(s) | 79, 171, 172 |
| AsuHPI | GGTGA | 1 cut(s) | 701 |
| AsuII | TTCGAA | 1 cut(s) | 104 |
| AvaI | CYCGRG | 1 cut(s) | 170 |
| AvaII | GGWCC | 1 cut(s) | 365 |
| BanI | GGYRCC | 1 cut(s) | 328 |
| BbsI | GAAGAC | 1 cut(s) | 407 |
| BbvI | GCAGC | 1 cut(s) | 578 |
| BccI | CCATC | 4 cut(s) | 282, 455, 464, 691 |
| BciT130I | CCWGG | 1 cut(s) | 420 |
| BcnI | CCSGG | 3 cut(s) | 79, 171, 172 |
| BfmI | CTRYAG | 1 cut(s) | 85 |
| BglII | AGATCT | 1 cut(s) | 161 |
| BisI | GCNGC | 1 cut(s) | 567 |
| BlsI | GCNGC | 1 cut(s) | 568 |
| Bme1390I | CCNGG | 4 cut(s) | 79, 171, 172, 420 |
| Bme18I | GGWCC | 1 cut(s) | 365 |
| BmeT110I | CYCGRG | 1 cut(s) | 170 |
| BmgT120I | GGNCC | 2 cut(s) | 173, 365 |
| BmiI | GGNNCC | 2 cut(s) | 330, 452 |
| BmrFI | CCNGG | 4 cut(s) | 79, 171, 172, 420 |
| BpiI | GAAGAC | 1 cut(s) | 407 |
| Bpu14I | TTCGAA | 1 cut(s) | 104 |
| BpuEI | CTTGAG | 1 cut(s) | 637 |
| BpuMI | CCSGG | 3 cut(s) | 79, 171, 172 |
| BsaBI | GATNNNNATC | 1 cut(s) | 701 |
| BsaJI | CCNNGG | 4 cut(s) | 78, 170, 176, 419 |
| BsaWI | WCCGGW | 1 cut(s) | 453 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 77, 177 |
| Bse8I | GATNNNNATC | 1 cut(s) | 701 |
| BseBI | CCWGG | 1 cut(s) | 420 |
| BseDI | CCNNGG | 4 cut(s) | 78, 170, 176, 419 |
| BseGI | GGATG | 1 cut(s) | 475 |
| BseJI | GATNNNNATC | 1 cut(s) | 701 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 77, 177 |
| BseMII | CTCAG | 2 cut(s) | 275, 635 |
| BseXI | GCAGC | 1 cut(s) | 578 |
| BsgI | GTGCAG | 1 cut(s) | 616 |
| Bsh1236I | CGCG | 1 cut(s) | 744 |
| BshFI | GGCC | 2 cut(s) | 175, 181 |
| BshNI | GGYRCC | 1 cut(s) | 328 |
| BsiHKCI | CYCGRG | 1 cut(s) | 170 |
| BsiSI | CCGG | 3 cut(s) | 78, 171, 454 |
| BslFI | GGGAC | 3 cut(s) | 95, 275, 738 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 77, 177 |
| BsmFI | GGGAC | 3 cut(s) | 95, 275, 738 |
| BsnI | GGCC | 2 cut(s) | 175, 181 |
| BsoBI | CYCGRG | 1 cut(s) | 170 |
| Bsp119I | TTCGAA | 1 cut(s) | 104 |
| Bsp1407I | TGTACA | 1 cut(s) | 135 |
| Bsp143I | GATC | 4 cut(s) | 39, 161, 547, 763 |
| Bsp19I | CCATGG | 1 cut(s) | 176 |
| BspACI | CCGC | 1 cut(s) | 664 |
| BspANI | GGCC | 2 cut(s) | 175, 181 |
| BspCNI | CTCAG | 2 cut(s) | 276, 636 |
| BspFNI | CGCG | 1 cut(s) | 744 |
| BspLI | GGNNCC | 2 cut(s) | 330, 452 |
| BspPI | GGATC | 2 cut(s) | 47, 542 |
| BspT104I | TTCGAA | 1 cut(s) | 104 |
| BspT107I | GGYRCC | 1 cut(s) | 328 |
| BsrGI | TGTACA | 1 cut(s) | 135 |
| BssECI | CCNNGG | 4 cut(s) | 78, 170, 176, 419 |
| BssMI | GATC | 4 cut(s) | 39, 161, 547, 763 |
| BssT1I | CCWWGG | 1 cut(s) | 176 |
| Bst2UI | CCWGG | 1 cut(s) | 420 |
| Bst4CI | ACNGT | 3 cut(s) | 253, 360, 573 |
| Bst6I | CTCTTC | 1 cut(s) | 198 |
| BstAUI | TGTACA | 1 cut(s) | 135 |
| BstBI | TTCGAA | 1 cut(s) | 104 |
| BstDEI | CTNAG | 3 cut(s) | 150, 284, 644 |
| BstDSI | CCRYGG | 1 cut(s) | 176 |
| BstF5I | GGATG | 1 cut(s) | 475 |
| BstFNI | CGCG | 1 cut(s) | 744 |
| BstKTI | GATC | 4 cut(s) | 42, 164, 550, 766 |
| BstMBI | GATC | 4 cut(s) | 39, 161, 547, 763 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 381 |
| BstNI | CCWGG | 1 cut(s) | 420 |
| BstSCI | CCNGG | 4 cut(s) | 77, 169, 170, 418 |
| BstSFI | CTRYAG | 1 cut(s) | 85 |
| BstUI | CGCG | 1 cut(s) | 744 |
| BstV1I | GCAGC | 1 cut(s) | 578 |
| BstV2I | GAAGAC | 1 cut(s) | 407 |
| BstX2I | RGATCY | 3 cut(s) | 39, 161, 547 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 339 |
| BstYI | RGATCY | 3 cut(s) | 39, 161, 547 |
| BsuRI | GGCC | 2 cut(s) | 175, 181 |
| BtgI | CCRYGG | 1 cut(s) | 176 |
| BtsCI | GGATG | 1 cut(s) | 475 |
| BtsIMutI | CAGTG | 1 cut(s) | 365 |
| Cfr13I | GGNCC | 2 cut(s) | 173, 365 |
| Cfr9I | CCCGGG | 1 cut(s) | 170 |
| Csp6I | GTAC | 1 cut(s) | 136 |
| CviAII | CATG | 1 cut(s) | 177 |
| CviJI | RGCY | 5 cut(s) | 97, 149, 175, 181, 384 |
| CviKI_1 | RGCY | 5 cut(s) | 97, 149, 175, 181, 384 |
| CviQI | GTAC | 1 cut(s) | 136 |
| DdeI | CTNAG | 3 cut(s) | 150, 284, 644 |
| DpnI | GATC | 4 cut(s) | 41, 163, 549, 765 |
| DpnII | GATC | 4 cut(s) | 39, 161, 547, 763 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 363 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 363 |
| Eam1104I | CTCTTC | 1 cut(s) | 198 |
| EarI | CTCTTC | 1 cut(s) | 198 |
| EciI | GGCGGA | 1 cut(s) | 679 |
| Eco130I | CCWWGG | 1 cut(s) | 176 |
| Eco47I | GGWCC | 1 cut(s) | 365 |
| Eco88I | CYCGRG | 1 cut(s) | 170 |
| EcoRII | CCWGG | 1 cut(s) | 418 |
| EcoT14I | CCWWGG | 1 cut(s) | 176 |
| ErhI | CCWWGG | 1 cut(s) | 176 |
| FaeI | CATG | 1 cut(s) | 180 |
| FaqI | GGGAC | 3 cut(s) | 95, 275, 738 |
| FatI | CATG | 1 cut(s) | 176 |
| FblI | GTMKAC | 1 cut(s) | 747 |
| Fnu4HI | GCNGC | 1 cut(s) | 567 |
| FokI | GGATG | 1 cut(s) | 482 |
| Fsp4HI | GCNGC | 1 cut(s) | 567 |
| GluI | GCNGC | 1 cut(s) | 567 |
| HaeIII | GGCC | 2 cut(s) | 175, 181 |
| HapII | CCGG | 3 cut(s) | 78, 171, 454 |
| Hin1II | CATG | 1 cut(s) | 180 |
| HincII | GTYRAC | 1 cut(s) | 579 |
| HindII | GTYRAC | 1 cut(s) | 579 |
| HpaI | GTTAAC | 1 cut(s) | 579 |
| HpaII | CCGG | 3 cut(s) | 78, 171, 454 |
| HphI | GGTGA | 1 cut(s) | 701 |
| Hpy166II | GTNNAC | 3 cut(s) | 136, 579, 748 |
| Hpy188I | TCNGA | 4 cut(s) | 61, 469, 645, 768 |
| Hpy188III | TCNNGA | 2 cut(s) | 551, 561 |
| Hpy8I | GTNNAC | 3 cut(s) | 136, 579, 748 |
| HpyAV | CCTTC | 3 cut(s) | 81, 523, 653 |
| HpyCH4III | ACNGT | 3 cut(s) | 253, 360, 573 |
| HpyCH4V | TGCA | 3 cut(s) | 593, 633, 712 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 381 |
| HpyF3I | CTNAG | 3 cut(s) | 150, 284, 644 |
| Hsp92II | CATG | 1 cut(s) | 180 |
| KspAI | GTTAAC | 1 cut(s) | 579 |
| Kzo9I | GATC | 4 cut(s) | 39, 161, 547, 763 |
| LpnPI | CCDG | 9 cut(s) | 91, 184, 307, 399, 405, 432, 467, 564, 643 |
| Lsp1109I | GCAGC | 1 cut(s) | 578 |
| MaeIII | GTNAC | 1 cut(s) | 344 |
| MalI | GATC | 4 cut(s) | 41, 163, 549, 765 |
| MboI | GATC | 4 cut(s) | 39, 161, 547, 763 |
| MboII | GAAGA | 3 cut(s) | 74, 215, 407 |
| MfeI | CAATTG | 1 cut(s) | 622 |
| MflI | RGATCY | 3 cut(s) | 39, 161, 547 |
| MluCI | AATT | 7 cut(s) | 16, 184, 243, 349, 489, 622, 758 |
| MmeI | TCCRAC | 2 cut(s) | 12, 447 |
| MnlI | CCTC | 3 cut(s) | 252, 307, 319 |
| MseI | TTAA | 4 cut(s) | 44, 543, 578, 707 |
| MslI | CAYNNNNRTG | 1 cut(s) | 707 |
| MspI | CCGG | 3 cut(s) | 78, 171, 454 |
| MspR9I | CCNGG | 4 cut(s) | 79, 171, 172, 420 |
| MunI | CAATTG | 1 cut(s) | 622 |
| MvaI | CCWGG | 1 cut(s) | 420 |
| MvnI | CGCG | 1 cut(s) | 744 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 381 |
| NciI | CCSGG | 3 cut(s) | 79, 171, 172 |
| NcoI | CCATGG | 1 cut(s) | 176 |
| NdeII | GATC | 4 cut(s) | 39, 161, 547, 763 |
| NlaIII | CATG | 1 cut(s) | 180 |
| NlaIV | GGNNCC | 2 cut(s) | 330, 452 |
| NmuCI | GTSAC | 1 cut(s) | 344 |
| NspV | TTCGAA | 1 cut(s) | 104 |
| PflFI | GACNNNGTC | 1 cut(s) | 752 |
| PkrI | GCNGC | 1 cut(s) | 568 |
| Psp6I | CCWGG | 1 cut(s) | 418 |
| PspGI | CCWGG | 1 cut(s) | 418 |
| PspN4I | GGNNCC | 2 cut(s) | 330, 452 |
| PspPI | GGNCC | 2 cut(s) | 173, 365 |
| PsuI | RGATCY | 3 cut(s) | 39, 161, 547 |
| PsyI | GACNNNGTC | 1 cut(s) | 752 |
| RsaI | GTAC | 1 cut(s) | 137 |
| RsaNI | GTAC | 1 cut(s) | 136 |
| RseI | CAYNNNNRTG | 1 cut(s) | 707 |
| SaqAI | TTAA | 4 cut(s) | 44, 543, 578, 707 |
| SatI | GCNGC | 1 cut(s) | 567 |
| Sau3AI | GATC | 4 cut(s) | 39, 161, 547, 763 |
| Sau96I | GGNCC | 2 cut(s) | 173, 365 |
| ScrFI | CCNGG | 4 cut(s) | 79, 171, 172, 420 |
| SetI | ASST | 3 cut(s) | 151, 330, 534 |
| SfcI | CTRYAG | 1 cut(s) | 85 |
| SfuI | TTCGAA | 1 cut(s) | 104 |
| SinI | GGWCC | 1 cut(s) | 365 |
| SmaI | CCCGGG | 1 cut(s) | 172 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 707 |
| SmlI | CTYRAG | 1 cut(s) | 616 |
| SmoI | CTYRAG | 1 cut(s) | 616 |
| Sse9I | AATT | 7 cut(s) | 16, 184, 243, 349, 489, 622, 758 |
| SsiI | CCGC | 1 cut(s) | 664 |
| SspI | AATATT | 2 cut(s) | 338, 394 |
| StyD4I | CCNGG | 4 cut(s) | 77, 169, 170, 418 |
| StyI | CCWWGG | 1 cut(s) | 176 |
| TaaI | ACNGT | 3 cut(s) | 253, 360, 573 |
| TaqI | TCGA | 3 cut(s) | 104, 560, 756 |
| TasI | AATT | 7 cut(s) | 16, 184, 243, 349, 489, 622, 758 |
| TatI | WGTACW | 1 cut(s) | 135 |
| Tru1I | TTAA | 4 cut(s) | 44, 543, 578, 707 |
| Tru9I | TTAA | 4 cut(s) | 44, 543, 578, 707 |
| TscAI | CASTG | 1 cut(s) | 365 |
| TseFI | GTSAC | 1 cut(s) | 344 |
| TseI | GCWGC | 1 cut(s) | 566 |
| Tsp45I | GTSAC | 1 cut(s) | 344 |
| TspDTI | ATGAA | 4 cut(s) | 17, 261, 321, 441 |
| TspMI | CCCGGG | 1 cut(s) | 170 |
| TspRI | CASTG | 1 cut(s) | 365 |
| Tth111I | GACNNNGTC | 1 cut(s) | 752 |
| VpaK11BI | GGWCC | 1 cut(s) | 365 |
| XmaI | CCCGGG | 1 cut(s) | 170 |
| XmiI | GTMKAC | 1 cut(s) | 747 |