Rw3G020740

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000112
Physical Location & Seq
Forward (+)
24671645 .. 24674874
3230 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G020740.1

Sequence Viewer

Length: 2259 bp
ATGCTGTTGAAATCTCTGCTTCTCTTCCTCCTCTTCCAAGTTTGCACTTCCAGGGACACCTTGAGATTGAACGAAGAACTCAAGGATGATGATGGTGGACTTCTGGTGTCCAAAGAAAGCAGCTTTGAGTTGGGTTTCTTCAGCCCCGGAAATTCTAGCAACTGCTATGTTGGGATTTGGTATTCTCAATCTAAAGTATCTGAAAAAACAGTGGTGTGGATCGCAAACAGAGACAACCCCATCAATGATACCTCTGGAGTTTTCACCATAAACAGGTATGGAGACCTAGTTCTTTATGCTTATAACATGGAGACCAGTCCTATTTGGTCTGCAGATGTGCCTTATTCAGTCAAAAACACTGGCACCTTATCTGCATATGTACAGCTGTTGGATACAGGAAGCTTAGTTATGTTCCAGAATGATAGAGATGGAATCTTTGTATGGCAAAGTTTTGATTATCCTACAGATACTTTAATCCCTGGTATGAAAGTTGGGATGAATTGGAAAACTGGGCTAGAATGGGTTTTAACATCTTGGAAGTCACTAGATGACCCTGGAACTGGGGACTATACCTTTAGGCTTCATAGTTGGAGCCACGTTGTCACACCCCAGTATTTTATTTATAAAGGTTTGTCTAAGTATTGGCGAGGTGATACAGAGCCTTGGCCTAATTCGATCCGAAATGCAGAGGAAATGTCTTTTTTCCTTACCTATAACACCAATAAACTTACAATAATGAAGTTGATGTTTTCTGGGCTACATCAGCACTTGGTATGGAGTGATGCTGACCATCAATGGAAGGAGTTGTTGTCTTCACCGAAATACAGGTGTGACTGGTATGGACATTGTGGTGCCAACAGCAAATGTAGCCTTGACAGTGGTAATCTGTTCGAGTGTGAAGATGGGTCAGATGGATGTGTGAGTAAACGAGTCAAAGTGTCACAGTGTAGAAATGGAGAAGGGTTTGTGAAGGTGGAAAGGGTGAAAGATCCAGACGCATCGAGAGCAGCATTGTCGAAAACAGGTATGACTACCAAAGAGTGTGAGCAGAAGTGCCTAAGAAATTGTTCTTGCACGGCATATATGACCAAAAAGATTGAAGGGGTTATTAATTGCTTGACGTGGTATGATGACTTGATGGACATTTTGGTTTACACAGAGGCCGGACGAGATCTGTATGTTCGTGTGGATAAAATTGAGTTAGCTGAGAATGCCGCAAACTCACAAGGATTCTTCAGGAGGGGCATGCTTGTTGTTCCAATACTGTTTGCTCTGCTGGCCCTAGTACTAATAAATGTGGATGAACTTGAGGAAAGCAAGAGACACCCGGAATTGCAATTTTTCGATCTGAACACACTAGTAGCAGCCACAGATAATTGCTCTGATGTTAATAAACTTGGCGAAGGTGGTTTTGGTGGTGTTTATAAGGGTGAATTACCAAATGGACAGAAGGTTGCTGTGAAAAGGCTGTCTAGCAGCTCAGGACAAGGGATTGGTGAATTCAAAAATGAAGCTGAACTGATAGCAGGACTTCAACACAGGAATCTTGTGAAGCTTTTAGGTTGTTGTATAAAGGGAGGAGAAAGGATGCTAGTCCTCGAATATATGCCGAACAAAAGCTTGGACTCATTTCTTTTTGATGACACAAGAAGGTCCTTCTTAGATTGGGGAAGGCGTTTTGAGATTATCAATGGGGTTGCGCGTGGGCTTTTGTATCTTCACCAAGACTCGAGATTAAGGATTATTCATAGGGATCTAAAAACCAGCAATATTCTTCTAGATGATGAGATGACCCCAAAAATCAATCTCAGATTTTTTGGCTACATGTCACCAGAGTATGCAGTGTTTGAGAGATTCTCTACGAAATCAGATGTGTTTAGTTTTGGGATTATACTATTGGAAATTGTTAGTGGCAAGAGAAACAATGGTTTTTATGAGGAGAATTACTCCATGAACCTGATAGGACATGTTTGGCATGTGTGGAAAGAAGGAATAGCCTTAGATATTGTGGATTCATCGCTACAATCGTATAAGCATGATGAAGTCTTGAGATGCATTCAAATTGGACTGTTGTGCGTGCAAGAAGATTCCAAGGACCGGCCAGCCATGTCATCCGTTGTGTTCATGTTGAGTGGTGAGGCATCTCTTCCATCTCCTAAGCAGCCTGCATTTGTCTTCAGAAGAAATTCCAGCAGTGCTGCTGATTCAAAAGAATTGCATTCTATTAACGACTTAACAATAACTACTGTGGGAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

752

Amino Acids

85.06

Weight (kDa)

5.67

Isoelectric Point (pI)

39.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 70 - 180 4.5e-33 D-mannose binding lectin
S_locus_glycop PF00954 230 - 302 1.9e-10 S-locus glycoprotein domain
PAN_2 PF08276 316 - 382 3e-18 PAN-like domain
Pkinase PF00069 463 - 643 8e-28 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 464 - 601 2.2e-27 Protein tyrosine and serine/threonine kinase
PK_Tyr_Ser-Thr PF07714 608 - 707 4.4e-07 Protein tyrosine and serine/threonine kinase
DUF3403 PF11883 713 - 750 6e-08 Domain of unknown function (DUF3403)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 303, 624, 1425
AccB1I GGYRCC 2 cut(s) 362, 851
AccII CGCG 1 cut(s) 1702
AciI CCGC 1 cut(s) 1215
AclWI GGATC 4 cut(s) 227, 670, 983, 1761
AcoI YGGCCR 1 cut(s) 2099
AcsI RAATTY 3 cut(s) 151, 1499, 2185
AcuI CTGAAG 3 cut(s) 124, 1219, 2161
AfaI GTAC 2 cut(s) 381, 1287
AfiI CCNNNNNNNGG 3 cut(s) 273, 560, 2097
AflIII ACRYGT 2 cut(s) 1824, 1966
AgsI TTSAA 7 cut(s) 10, 70, 1100, 1504, 1535, 2060, 2208
AhlI ACTAGT 1 cut(s) 1357
AjiI CACGTC 1 cut(s) 1122
AjnI CCWGG 3 cut(s) 50, 478, 553
AjuI GAANNNNNNNTTGG 4 cut(s) 1395, 1427, 1604, 1636
AloI GAACNNNNNNTCC 2 cut(s) 273, 305
AluBI AGCT 9 cut(s) 123, 385, 402, 1205, 1479, 1514, 1555, 1620, 2255
AluI AGCT 9 cut(s) 123, 385, 402, 1205, 1479, 1514, 1555, 1620, 2255
Alw26I GTCTC 4 cut(s) 225, 276, 305, 1315
AlwI GGATC 4 cut(s) 227, 670, 983, 1761
Ama87I CYCGRG 1 cut(s) 1729
AoxI GGCC 4 cut(s) 665, 1161, 1278, 2099
ApeKI GCWGC 6 cut(s) 120, 1007, 1364, 1476, 2161, 2198
ApoI RAATTY 3 cut(s) 151, 1499, 2185
AseI ATTAAT 1 cut(s) 1110
Asp700I GAANNNNTTC 2 cut(s) 1066, 2185
AspLEI GCGC 1 cut(s) 1702
AspS9I GGNCC 3 cut(s) 1279, 1653, 2095
AsuC2I CCSGG 2 cut(s) 147, 1328
AsuHPI GGTGA 9 cut(s) 256, 662, 807, 994, 1442, 1508, 1712, 1821, 2147
AvaI CYCGRG 1 cut(s) 1729
AvaII GGWCC 2 cut(s) 1653, 2095
BanI GGYRCC 2 cut(s) 362, 851
BbsI GAAGAC 2 cut(s) 804, 2167
BbvI GCAGC 6 cut(s) 132, 1019, 1376, 1488, 2173, 2185
BccI CCATC 8 cut(s) 86, 248, 422, 798, 896, 905, 1132, 2158
BceAI ACGGC 1 cut(s) 1092
BcgI CGANNNNNNTGC 2 cut(s) 996, 1030
BciT130I CCWGG 3 cut(s) 52, 480, 555
BciVI GTATCC 1 cut(s) 385
BcnI CCSGG 2 cut(s) 147, 1328
BcoDI GTCTC 4 cut(s) 225, 276, 305, 1315
BcuI ACTAGT 1 cut(s) 1357
BfaI CTAG 9 cut(s) 156, 287, 515, 545, 1283, 1358, 1473, 1592, 1778
BfmI CTRYAG 2 cut(s) 330, 462
BfuI GTATCC 1 cut(s) 385
BglII AGATCT 1 cut(s) 1171
BisI GCNGC 7 cut(s) 121, 1008, 1215, 1365, 1477, 2162, 2199
BlsI GCNGC 7 cut(s) 122, 1009, 1216, 1366, 1478, 2163, 2200
BmcAI AGTACT 1 cut(s) 1287
Bme1390I CCNGG 5 cut(s) 52, 147, 480, 555, 1328
Bme18I GGWCC 2 cut(s) 1653, 2095
BmeT110I CYCGRG 1 cut(s) 1729
BmgBI CACGTC 1 cut(s) 1122
BmgT120I GGNCC 3 cut(s) 1279, 1653, 2095
BmiI GGNNCC 3 cut(s) 364, 593, 853
BmrFI CCNGG 5 cut(s) 52, 147, 480, 555, 1328
BmrI ACTGGG 3 cut(s) 519, 570, 604
BmsI GCATC 5 cut(s) 772, 1007, 1578, 2042, 2150
BmuI ACTGGG 3 cut(s) 519, 570, 604
BpiI GAAGAC 2 cut(s) 804, 2167
BplI GAGNNNNNCTC 4 cut(s) 1841, 1873, 1931, 1963
BpmI CTGGAG 1 cut(s) 276
Bpu10I CCTNAGC 2 cut(s) 1480, 2157
BpuEI CTTGAG 4 cut(s) 65, 82, 1328, 2068
BpuMI CCSGG 2 cut(s) 147, 1328
BsaI GGTCTC 2 cut(s) 276, 305
BsaJI CCNNGG 6 cut(s) 51, 145, 478, 553, 662, 2091
BsaXI ACNNNNNCTCC 4 cut(s) 273, 303, 794, 824
Bsc4I CCNNNNNNNGG 3 cut(s) 273, 560, 2097
Bse118I RCCGGY 1 cut(s) 2097
Bse1I ACTGG 6 cut(s) 315, 364, 514, 565, 610, 839
BseBI CCWGG 3 cut(s) 52, 480, 555
BseDI CCNNGG 6 cut(s) 51, 145, 478, 553, 662, 2091
BseGI GGATG 6 cut(s) 91, 501, 920, 1306, 1593, 2111
BseLI CCNNNNNNNGG 3 cut(s) 273, 560, 2097
BseMII CTCAG 3 cut(s) 1197, 1494, 1822
BseNI ACTGG 6 cut(s) 315, 364, 514, 565, 610, 839
BseRI GAGGAG 3 cut(s) 20, 1593, 1952
BseXI GCAGC 6 cut(s) 132, 1019, 1376, 1488, 2173, 2185
Bsh1236I CGCG 1 cut(s) 1702
BshFI GGCC 4 cut(s) 667, 1163, 1280, 2101
BshNI GGYRCC 2 cut(s) 362, 851
BsiHKCI CYCGRG 1 cut(s) 1729
BsiSI CCGG 4 cut(s) 147, 1164, 1328, 2098
BslFI GGGAC 2 cut(s) 68, 578
BslI CCNNNNNNNGG 3 cut(s) 273, 560, 2097
BsmAI GTCTC 4 cut(s) 225, 276, 305, 1315
BsmFI GGGAC 2 cut(s) 68, 578
BsmI GAATGC 3 cut(s) 1216, 2055, 2218
BsnI GGCC 4 cut(s) 667, 1163, 1280, 2101
Bso31I GGTCTC 2 cut(s) 276, 305
BsoBI CYCGRG 1 cut(s) 1729
Bsp1407I TGTACA 1 cut(s) 379
Bsp143I GATC 6 cut(s) 219, 675, 988, 1171, 1345, 1753
BspACI CCGC 1 cut(s) 1215
BspANI GGCC 4 cut(s) 667, 1163, 1280, 2101
BspCNI CTCAG 3 cut(s) 1198, 1493, 1821
BspFNI CGCG 1 cut(s) 1702
BspLI GGNNCC 3 cut(s) 364, 593, 853
BspMAI CTGCAG 1 cut(s) 334
BspPI GGATC 4 cut(s) 227, 670, 983, 1761
BspT107I GGYRCC 2 cut(s) 362, 851
BspTNI GGTCTC 2 cut(s) 276, 305
BsrFI RCCGGY 1 cut(s) 2097
BsrGI TGTACA 1 cut(s) 379
BsrI ACTGG 6 cut(s) 315, 364, 514, 565, 610, 839
BssAI RCCGGY 1 cut(s) 2097
BssECI CCNNGG 6 cut(s) 51, 145, 478, 553, 662, 2091
BssMI GATC 6 cut(s) 219, 675, 988, 1171, 1345, 1753
BssT1I CCWWGG 2 cut(s) 662, 2091
Bst2UI CCWGG 3 cut(s) 52, 480, 555
Bst4CI ACNGT 6 cut(s) 211, 878, 945, 1266, 2070, 2248
Bst6I CTCTTC 3 cut(s) 29, 38, 2151
BstAUI TGTACA 1 cut(s) 379
BstC8I GCNNGC 5 cut(s) 1247, 1278, 2078, 2103, 2166
BstDEI CTNAG 9 cut(s) 403, 636, 1058, 1206, 1480, 1660, 1808, 1999, 2157
BstF5I GGATG 6 cut(s) 91, 501, 920, 1306, 1593, 2111
BstFNI CGCG 1 cut(s) 1702
BstHHI GCGC 1 cut(s) 1702
BstKTI GATC 6 cut(s) 222, 678, 991, 1174, 1348, 1756
BstMAI GTCTC 4 cut(s) 225, 276, 305, 1315
BstMBI GATC 6 cut(s) 219, 675, 988, 1171, 1345, 1753
BstMWI GCNNNNNNNGC 5 cut(s) 763, 867, 1004, 1211, 1277
BstNI CCWGG 3 cut(s) 52, 480, 555
BstNSI RCATGY 4 cut(s) 1249, 1828, 1970, 1979
BstSCI CCNGG 5 cut(s) 50, 145, 478, 553, 1326
BstSFI CTRYAG 2 cut(s) 330, 462
BstUI CGCG 1 cut(s) 1702
BstV1I GCAGC 6 cut(s) 132, 1019, 1376, 1488, 2173, 2185
BstV2I GAAGAC 2 cut(s) 804, 2167
BstX2I RGATCY 3 cut(s) 988, 1171, 1753
BstYI RGATCY 3 cut(s) 988, 1171, 1753
BsuI GTATCC 1 cut(s) 385
BsuRI GGCC 4 cut(s) 667, 1163, 1280, 2101
BtgZI GCGATG 1 cut(s) 2001
BtrI CACGTC 1 cut(s) 1122
BtsCI GGATG 6 cut(s) 91, 501, 920, 1306, 1593, 2111
BtsI GCAGTG 2 cut(s) 1848, 2200
BtsIMutI CAGTG 6 cut(s) 216, 357, 883, 950, 1848, 2200
Cac8I GCNNGC 5 cut(s) 1247, 1278, 2078, 2103, 2166
CfoI GCGC 1 cut(s) 1702
Cfr10I RCCGGY 1 cut(s) 2097
Cfr13I GGNCC 3 cut(s) 1279, 1653, 2095
CseI GACGC 1 cut(s) 1004
Csp6I GTAC 2 cut(s) 380, 1286
CviAII CATG 9 cut(s) 307, 1246, 1825, 1951, 1967, 1976, 2036, 2107, 2125
CviQI GTAC 2 cut(s) 380, 1286
DdeI CTNAG 9 cut(s) 403, 636, 1058, 1206, 1480, 1660, 1808, 1999, 2157
DpnI GATC 6 cut(s) 221, 677, 990, 1173, 1347, 1755
DpnII GATC 6 cut(s) 219, 675, 988, 1171, 1345, 1753
EaeI YGGCCR 1 cut(s) 2099
Eam1104I CTCTTC 3 cut(s) 29, 38, 2151
EarI CTCTTC 3 cut(s) 29, 38, 2151
Eco130I CCWWGG 2 cut(s) 662, 2091
Eco31I GGTCTC 2 cut(s) 276, 305
Eco47I GGWCC 2 cut(s) 1653, 2095
Eco57I CTGAAG 3 cut(s) 124, 1219, 2161
Eco88I CYCGRG 1 cut(s) 1729
EcoO109I RGGNCCY 1 cut(s) 1653
EcoRI GAATTC 1 cut(s) 1499
EcoRII CCWGG 3 cut(s) 50, 478, 553
EcoT14I CCWWGG 2 cut(s) 662, 2091
EcoT22I ATGCAT 1 cut(s) 2057
ErhI CCWWGG 2 cut(s) 662, 2091
FaeI CATG 9 cut(s) 310, 1249, 1828, 1954, 1970, 1979, 2039, 2110, 2128
FaqI GGGAC 2 cut(s) 68, 578
FatI CATG 9 cut(s) 306, 1245, 1824, 1950, 1966, 1975, 2035, 2106, 2124
FauNDI CATATG 1 cut(s) 376
Fnu4HI GCNGC 7 cut(s) 121, 1008, 1215, 1365, 1477, 2162, 2199
FokI GGATG 6 cut(s) 98, 508, 927, 1313, 1600, 2098
Fsp4HI GCNGC 7 cut(s) 121, 1008, 1215, 1365, 1477, 2162, 2199
FspBI CTAG 9 cut(s) 156, 287, 515, 545, 1283, 1358, 1473, 1592, 1778
GlaI GCGC 1 cut(s) 1701
GluI GCNGC 7 cut(s) 121, 1008, 1215, 1365, 1477, 2162, 2199
GsuI CTGGAG 1 cut(s) 276
HaeIII GGCC 4 cut(s) 667, 1163, 1280, 2101
HapII CCGG 4 cut(s) 147, 1164, 1328, 2098
HgaI GACGC 1 cut(s) 1004
HhaI GCGC 1 cut(s) 1702
Hin1II CATG 9 cut(s) 310, 1249, 1828, 1954, 1970, 1979, 2039, 2110, 2128
Hin6I GCGC 1 cut(s) 1700
HinP1I GCGC 1 cut(s) 1700
HindIII AAGCTT 3 cut(s) 400, 1553, 1618
HpaII CCGG 4 cut(s) 147, 1164, 1328, 2098
HphI GGTGA 9 cut(s) 256, 662, 807, 994, 1442, 1508, 1712, 1821, 2147
Hpy166II GTNNAC 3 cut(s) 98, 926, 1153
Hpy188I TCNGA 8 cut(s) 202, 680, 910, 1350, 1384, 1811, 1870, 2180
Hpy188III TCNNGA 9 cut(s) 255, 415, 992, 1002, 1237, 1482, 1731, 1778, 2047
Hpy8I GTNNAC 3 cut(s) 98, 926, 1153
HpyCH4III ACNGT 6 cut(s) 211, 878, 945, 1266, 2070, 2248
HpyCH4IV ACGT 2 cut(s) 597, 1121
HpyF10VI GCNNNNNNNGC 5 cut(s) 763, 867, 1004, 1211, 1277
HpyF3I CTNAG 9 cut(s) 403, 636, 1058, 1206, 1480, 1660, 1808, 1999, 2157
HpySE526I ACGT 2 cut(s) 597, 1121
Hsp92II CATG 9 cut(s) 310, 1249, 1828, 1954, 1970, 1979, 2039, 2110, 2128
HspAI GCGC 1 cut(s) 1700
Kzo9I GATC 6 cut(s) 219, 675, 988, 1171, 1345, 1753
LmnI GCTCC 2 cut(s) 591, 2252
Lsp1109I GCAGC 6 cut(s) 132, 1019, 1376, 1488, 2173, 2185
LweI GCATC 5 cut(s) 772, 1007, 1578, 2042, 2150
MaeI CTAG 9 cut(s) 156, 287, 515, 545, 1283, 1358, 1473, 1592, 1778
MaeII ACGT 2 cut(s) 597, 1121
MaeIII GTNAC 5 cut(s) 540, 601, 830, 939, 1827
MalI GATC 6 cut(s) 221, 677, 990, 1173, 1347, 1755
MboI GATC 6 cut(s) 219, 675, 988, 1171, 1345, 1753
MflI RGATCY 3 cut(s) 988, 1171, 1753
MlyI GAGTC 3 cut(s) 939, 1619, 1721
MmeI TCCRAC 2 cut(s) 369, 569
Mph1103I ATGCAT 1 cut(s) 2057
MroXI GAANNNNTTC 2 cut(s) 1066, 2185
MseI TTAA 7 cut(s) 473, 527, 1110, 1389, 1736, 2226, 2234
MslI CAYNNNNRTG 1 cut(s) 849
MspA1I CMGCKG 1 cut(s) 385
MspI CCGG 4 cut(s) 147, 1164, 1328, 2098
MspR9I CCNGG 5 cut(s) 52, 147, 480, 555, 1328
Mva1269I GAATGC 3 cut(s) 1216, 2055, 2218
MvaI CCWGG 3 cut(s) 52, 480, 555
MvnI CGCG 1 cut(s) 1702
MwoI GCNNNNNNNGC 5 cut(s) 763, 867, 1004, 1211, 1277
NciI CCSGG 2 cut(s) 147, 1328
NdeI CATATG 1 cut(s) 376
NdeII GATC 6 cut(s) 219, 675, 988, 1171, 1345, 1753
NlaIII CATG 9 cut(s) 310, 1249, 1828, 1954, 1970, 1979, 2039, 2110, 2128
NlaIV GGNNCC 3 cut(s) 364, 593, 853
NmuCI GTSAC 5 cut(s) 540, 601, 830, 939, 1827
NsiI ATGCAT 1 cut(s) 2057
NspI RCATGY 4 cut(s) 1249, 1828, 1970, 1979
PaeI GCATGC 1 cut(s) 1249
PaeR7I CTCGAG 1 cut(s) 1729
PciI ACATGT 2 cut(s) 1824, 1966
PcsI WCGNNNNNNNCGW 1 cut(s) 2024
PctI GAATGC 3 cut(s) 1216, 2055, 2218
PdmI GAANNNNTTC 2 cut(s) 1066, 2185
PfeI GAWTC 7 cut(s) 432, 1230, 1543, 1854, 2012, 2087, 2204
PkrI GCNGC 7 cut(s) 122, 1009, 1216, 1366, 1478, 2163, 2200
PleI GAGTC 3 cut(s) 938, 1619, 1721
PpsI GAGTC 3 cut(s) 938, 1619, 1721
PpuMI RGGWCCY 1 cut(s) 1653
PscI ACATGT 2 cut(s) 1824, 1966
PshBI ATTAAT 1 cut(s) 1110
PsiI TTATAA 3 cut(s) 303, 624, 1425
Psp5II RGGWCCY 1 cut(s) 1653
Psp6I CCWGG 3 cut(s) 50, 478, 553
PspGI CCWGG 3 cut(s) 50, 478, 553
PspN4I GGNNCC 3 cut(s) 364, 593, 853
PspPI GGNCC 3 cut(s) 1279, 1653, 2095
PspPPI RGGWCCY 1 cut(s) 1653
PstI CTGCAG 1 cut(s) 334
PsuI RGATCY 3 cut(s) 988, 1171, 1753
PvuII CAGCTG 1 cut(s) 385
RsaI GTAC 2 cut(s) 381, 1287
RsaNI GTAC 2 cut(s) 380, 1286
RseI CAYNNNNRTG 1 cut(s) 849
SaqAI TTAA 7 cut(s) 473, 527, 1110, 1389, 1736, 2226, 2234
SatI GCNGC 7 cut(s) 121, 1008, 1215, 1365, 1477, 2162, 2199
Sau3AI GATC 6 cut(s) 219, 675, 988, 1171, 1345, 1753
Sau96I GGNCC 3 cut(s) 1279, 1653, 2095
ScaI AGTACT 1 cut(s) 1287
SchI GAGTC 3 cut(s) 939, 1619, 1721
ScrFI CCNGG 5 cut(s) 52, 147, 480, 555, 1328
SfaNI GCATC 5 cut(s) 772, 1007, 1578, 2042, 2150
SfcI CTRYAG 2 cut(s) 330, 462
Sfr274I CTCGAG 1 cut(s) 1729
SinI GGWCC 2 cut(s) 1653, 2095
SlaI CTCGAG 1 cut(s) 1729
SmiMI CAYNNNNRTG 1 cut(s) 849
SmlI CTYRAG 5 cut(s) 61, 80, 1307, 1729, 2047
SmoI CTYRAG 5 cut(s) 61, 80, 1307, 1729, 2047
SpeI ACTAGT 1 cut(s) 1357
SphI GCATGC 1 cut(s) 1249
SsiI CCGC 1 cut(s) 1215
SspI AATATT 1 cut(s) 1771
SspMI CTAG 9 cut(s) 156, 287, 515, 545, 1283, 1358, 1473, 1592, 1778
StyD4I CCNGG 5 cut(s) 50, 145, 478, 553, 1326
StyI CCWWGG 2 cut(s) 662, 2091
TaaI ACNGT 6 cut(s) 211, 878, 945, 1266, 2070, 2248
TaiI ACGT 2 cut(s) 600, 1124
TaqI TCGA 7 cut(s) 674, 891, 1001, 1016, 1344, 1599, 1730
TatI WGTACW 2 cut(s) 379, 1285
TauI GCSGC 1 cut(s) 1217
TfiI GAWTC 7 cut(s) 432, 1230, 1543, 1854, 2012, 2087, 2204
Tru1I TTAA 7 cut(s) 473, 527, 1110, 1389, 1736, 2226, 2234
Tru9I TTAA 7 cut(s) 473, 527, 1110, 1389, 1736, 2226, 2234
TscAI CASTG 6 cut(s) 216, 364, 883, 950, 1848, 2200
TseFI GTSAC 5 cut(s) 540, 601, 830, 939, 1827
TseI GCWGC 6 cut(s) 120, 1007, 1364, 1476, 2161, 2198
Tsp45I GTSAC 5 cut(s) 540, 601, 830, 939, 1827
TspGWI ACGGA 1 cut(s) 2104
TspRI CASTG 6 cut(s) 216, 364, 883, 950, 1848, 2200
VpaK11BI GGWCC 2 cut(s) 1653, 2095
VspI ATTAAT 1 cut(s) 1110
XapI RAATTY 3 cut(s) 151, 1499, 2185
XbaI TCTAGA 1 cut(s) 1777
XceI RCATGY 4 cut(s) 1249, 1828, 1970, 1979
XhoI CTCGAG 1 cut(s) 1729
XmnI GAANNNNTTC 2 cut(s) 1066, 2185
XspI CTAG 9 cut(s) 156, 287, 515, 545, 1283, 1358, 1473, 1592, 1778
ZrmI AGTACT 1 cut(s) 1287
Zsp2I ATGCAT 1 cut(s) 2057
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.