divergent subfamily of APPLE domains
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 177 |
| Acc36I | ACCTGC | 1 cut(s) | 850 |
| AccB1I | GGYRCC | 1 cut(s) | 737 |
| AcoI | YGGCCR | 1 cut(s) | 506 |
| AcsI | RAATTY | 4 cut(s) | 57, 243, 612, 831 |
| AcuI | CTGAAG | 3 cut(s) | 219, 632, 727 |
| AcyI | GRCGYC | 1 cut(s) | 738 |
| AfaI | GTAC | 4 cut(s) | 232, 529, 870, 1135 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 416 |
| AflIII | ACRYGT | 1 cut(s) | 907 |
| AgsI | TTSAA | 5 cut(s) | 355, 480, 618, 770, 1014 |
| AhlI | ACTAGT | 1 cut(s) | 227 |
| AjnI | CCWGG | 5 cut(s) | 28, 334, 389, 409, 790 |
| AleI | CACNNNNGTG | 1 cut(s) | 206 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 147, 179 |
| AluBI | AGCT | 6 cut(s) | 13, 239, 306, 378, 916, 938 |
| AluI | AGCT | 6 cut(s) | 13, 239, 306, 378, 916, 938 |
| Alw26I | GTCTC | 1 cut(s) | 356 |
| AoxI | GGCC | 1 cut(s) | 506 |
| ApeKI | GCWGC | 1 cut(s) | 698 |
| ApoI | RAATTY | 4 cut(s) | 57, 243, 612, 831 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 539, 571 |
| AseI | ATTAAT | 1 cut(s) | 120 |
| AspLEI | GCGC | 1 cut(s) | 740 |
| AspS9I | GGNCC | 2 cut(s) | 198, 271 |
| AsuHPI | GGTGA | 2 cut(s) | 513, 971 |
| AsuII | TTCGAA | 1 cut(s) | 493 |
| AvaII | GGWCC | 2 cut(s) | 198, 271 |
| BalI | TGGCCA | 1 cut(s) | 508 |
| BanI | GGYRCC | 1 cut(s) | 737 |
| BanII | GRGCYC | 1 cut(s) | 518 |
| BbsI | GAAGAC | 1 cut(s) | 294 |
| BbvI | GCAGC | 1 cut(s) | 685 |
| BccI | CCATC | 3 cut(s) | 526, 836, 1022 |
| BciT130I | CCWGG | 5 cut(s) | 30, 336, 391, 411, 792 |
| BciVI | GTATCC | 1 cut(s) | 316 |
| BcoDI | GTCTC | 1 cut(s) | 356 |
| BcuI | ACTAGT | 1 cut(s) | 227 |
| BfaI | CTAG | 5 cut(s) | 14, 39, 228, 483, 1019 |
| BfoI | RGCGCY | 1 cut(s) | 741 |
| BfuAI | ACCTGC | 1 cut(s) | 850 |
| BfuI | GTATCC | 1 cut(s) | 316 |
| BisI | GCNGC | 1 cut(s) | 699 |
| BlsI | GCNGC | 1 cut(s) | 700 |
| BmcAI | AGTACT | 1 cut(s) | 232 |
| Bme1390I | CCNGG | 5 cut(s) | 30, 336, 391, 411, 792 |
| Bme18I | GGWCC | 2 cut(s) | 198, 271 |
| BmgT120I | GGNCC | 2 cut(s) | 198, 271 |
| BmiI | GGNNCC | 3 cut(s) | 451, 515, 739 |
| BmrFI | CCNGG | 5 cut(s) | 30, 336, 391, 411, 792 |
| BmsI | GCATC | 4 cut(s) | 321, 913, 935, 1108 |
| BpiI | GAAGAC | 1 cut(s) | 294 |
| BpmI | CTGGAG | 3 cut(s) | 412, 438, 795 |
| Bpu14I | TTCGAA | 1 cut(s) | 493 |
| BsaHI | GRCGYC | 1 cut(s) | 738 |
| BsaJI | CCNNGG | 4 cut(s) | 28, 409, 509, 791 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 416 |
| Bse1I | ACTGG | 4 cut(s) | 86, 421, 451, 1086 |
| BseBI | CCWGG | 5 cut(s) | 30, 336, 391, 411, 792 |
| BseDI | CCNNGG | 4 cut(s) | 28, 409, 509, 791 |
| BseGI | GGATG | 2 cut(s) | 386, 755 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 416 |
| BseMII | CTCAG | 3 cut(s) | 765, 816, 1116 |
| BseNI | ACTGG | 4 cut(s) | 86, 421, 451, 1086 |
| BseXI | GCAGC | 1 cut(s) | 685 |
| BsgI | GTGCAG | 1 cut(s) | 684 |
| BshFI | GGCC | 1 cut(s) | 508 |
| BshNI | GGYRCC | 1 cut(s) | 737 |
| BslFI | GGGAC | 2 cut(s) | 122, 1107 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 416 |
| BsmAI | GTCTC | 1 cut(s) | 356 |
| BsmFI | GGGAC | 2 cut(s) | 122, 1107 |
| BsnI | GGCC | 1 cut(s) | 508 |
| Bsp119I | TTCGAA | 1 cut(s) | 493 |
| Bsp1286I | GDGCHC | 1 cut(s) | 518 |
| Bsp19I | CCATGG | 1 cut(s) | 509 |
| BspANI | GGCC | 1 cut(s) | 508 |
| BspCNI | CTCAG | 3 cut(s) | 766, 817, 1117 |
| BspLI | GGNNCC | 3 cut(s) | 451, 515, 739 |
| BspMI | ACCTGC | 1 cut(s) | 850 |
| BspT104I | TTCGAA | 1 cut(s) | 493 |
| BspT107I | GGYRCC | 1 cut(s) | 737 |
| BsrI | ACTGG | 4 cut(s) | 86, 421, 451, 1086 |
| BssECI | CCNNGG | 4 cut(s) | 28, 409, 509, 791 |
| BssNI | GRCGYC | 1 cut(s) | 738 |
| BssT1I | CCWWGG | 1 cut(s) | 509 |
| Bst2UI | CCWGG | 5 cut(s) | 30, 336, 391, 411, 792 |
| Bst4CI | ACNGT | 1 cut(s) | 284 |
| BstACI | GRCGYC | 1 cut(s) | 738 |
| BstBI | TTCGAA | 1 cut(s) | 493 |
| BstC8I | GCNNGC | 1 cut(s) | 982 |
| BstDEI | CTNAG | 3 cut(s) | 774, 825, 1125 |
| BstDSI | CCRYGG | 1 cut(s) | 509 |
| BstF5I | GGATG | 2 cut(s) | 386, 755 |
| BstH2I | RGCGCY | 1 cut(s) | 741 |
| BstHHI | GCGC | 1 cut(s) | 740 |
| BstMAI | GTCTC | 1 cut(s) | 356 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 24 |
| BstNI | CCWGG | 5 cut(s) | 30, 336, 391, 411, 792 |
| BstNSI | RCATGY | 2 cut(s) | 911, 984 |
| BstSCI | CCNGG | 5 cut(s) | 28, 334, 389, 409, 790 |
| BstV1I | GCAGC | 1 cut(s) | 685 |
| BstV2I | GAAGAC | 1 cut(s) | 294 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 93, 819 |
| BsuI | GTATCC | 1 cut(s) | 316 |
| BsuRI | GGCC | 1 cut(s) | 508 |
| BtgI | CCRYGG | 1 cut(s) | 509 |
| BtsCI | GGATG | 2 cut(s) | 386, 755 |
| BtsIMutI | CAGTG | 3 cut(s) | 93, 289, 751 |
| BveI | ACCTGC | 1 cut(s) | 850 |
| Cac8I | GCNNGC | 1 cut(s) | 982 |
| CfoI | GCGC | 1 cut(s) | 740 |
| Cfr13I | GGNCC | 2 cut(s) | 198, 271 |
| CseI | GACGC | 1 cut(s) | 991 |
| Csp6I | GTAC | 4 cut(s) | 231, 528, 869, 1134 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 606, 641 |
| CviAII | CATG | 7 cut(s) | 510, 660, 908, 981, 999, 1045, 1050 |
| CviQI | GTAC | 4 cut(s) | 231, 528, 869, 1134 |
| DdeI | CTNAG | 3 cut(s) | 774, 825, 1125 |
| DinI | GGCGCC | 1 cut(s) | 739 |
| EaeI | YGGCCR | 1 cut(s) | 506 |
| Eco130I | CCWWGG | 1 cut(s) | 509 |
| Eco24I | GRGCYC | 1 cut(s) | 518 |
| Eco47I | GGWCC | 2 cut(s) | 198, 271 |
| Eco57I | CTGAAG | 3 cut(s) | 219, 632, 727 |
| EcoRI | GAATTC | 1 cut(s) | 57 |
| EcoRII | CCWGG | 5 cut(s) | 28, 334, 389, 409, 790 |
| EcoT14I | CCWWGG | 1 cut(s) | 509 |
| EcoT22I | ATGCAT | 2 cut(s) | 544, 928 |
| EcoT38I | GRGCYC | 1 cut(s) | 518 |
| EgeI | GGCGCC | 1 cut(s) | 739 |
| EheI | GGCGCC | 1 cut(s) | 739 |
| ErhI | CCWWGG | 1 cut(s) | 509 |
| FaeI | CATG | 7 cut(s) | 513, 663, 911, 984, 1002, 1048, 1053 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 170, 202 |
| FaqI | GGGAC | 2 cut(s) | 122, 1107 |
| FatI | CATG | 7 cut(s) | 509, 659, 907, 980, 998, 1044, 1049 |
| FauNDI | CATATG | 1 cut(s) | 544 |
| Fnu4HI | GCNGC | 1 cut(s) | 699 |
| FokI | GGATG | 2 cut(s) | 373, 762 |
| FriOI | GRGCYC | 1 cut(s) | 518 |
| Fsp4HI | GCNGC | 1 cut(s) | 699 |
| FspBI | CTAG | 5 cut(s) | 14, 39, 228, 483, 1019 |
| GlaI | GCGC | 1 cut(s) | 739 |
| GluI | GCNGC | 1 cut(s) | 699 |
| GsuI | CTGGAG | 3 cut(s) | 412, 438, 795 |
| HaeII | RGCGCY | 1 cut(s) | 741 |
| HaeIII | GGCC | 1 cut(s) | 508 |
| HgaI | GACGC | 1 cut(s) | 991 |
| HhaI | GCGC | 1 cut(s) | 740 |
| Hin1I | GRCGYC | 1 cut(s) | 738 |
| Hin1II | CATG | 7 cut(s) | 513, 663, 911, 984, 1002, 1048, 1053 |
| Hin6I | GCGC | 1 cut(s) | 738 |
| HinP1I | GCGC | 1 cut(s) | 738 |
| HincII | GTYRAC | 1 cut(s) | 631 |
| HindII | GTYRAC | 1 cut(s) | 631 |
| HindIII | AAGCTT | 2 cut(s) | 304, 914 |
| HinfI | GANTC | 4 cut(s) | 125, 355, 590, 1127 |
| HpaI | GTTAAC | 1 cut(s) | 631 |
| HphI | GGTGA | 2 cut(s) | 513, 971 |
| Hpy166II | GTNNAC | 3 cut(s) | 201, 631, 1069 |
| Hpy188I | TCNGA | 7 cut(s) | 117, 214, 400, 610, 775, 826, 841 |
| Hpy8I | GTNNAC | 3 cut(s) | 201, 631, 1069 |
| Hpy99I | CGWCG | 1 cut(s) | 1007 |
| HpyAV | CCTTC | 2 cut(s) | 679, 751 |
| HpyCH4III | ACNGT | 1 cut(s) | 284 |
| HpyCH4V | TGCA | 6 cut(s) | 542, 701, 926, 972, 1044, 1121 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 24 |
| HpyF3I | CTNAG | 3 cut(s) | 774, 825, 1125 |
| Hsp92I | GRCGYC | 1 cut(s) | 738 |
| Hsp92II | CATG | 7 cut(s) | 513, 663, 911, 984, 1002, 1048, 1053 |
| HspAI | GCGC | 1 cut(s) | 738 |
| KasI | GGCGCC | 1 cut(s) | 737 |
| KspAI | GTTAAC | 1 cut(s) | 631 |
| LmnI | GCTCC | 2 cut(s) | 455, 513 |
| Lsp1109I | GCAGC | 1 cut(s) | 685 |
| LweI | GCATC | 4 cut(s) | 321, 913, 935, 1108 |
| MaeI | CTAG | 5 cut(s) | 14, 39, 228, 483, 1019 |
| MaeIII | GTNAC | 3 cut(s) | 278, 574, 716 |
| MboII | GAAGA | 3 cut(s) | 299, 413, 585 |
| MhlI | GDGCHC | 1 cut(s) | 518 |
| MlsI | TGGCCA | 1 cut(s) | 508 |
| MluNI | TGGCCA | 1 cut(s) | 508 |
| Mly113I | GGCGCC | 1 cut(s) | 738 |
| MlyI | GAGTC | 1 cut(s) | 1136 |
| MmeI | TCCRAC | 2 cut(s) | 34, 341 |
| MnlI | CCTC | 7 cut(s) | 149, 387, 616, 667, 891, 969, 1025 |
| Mox20I | TGGCCA | 1 cut(s) | 508 |
| Mph1103I | ATGCAT | 2 cut(s) | 544, 928 |
| MscI | TGGCCA | 1 cut(s) | 508 |
| MseI | TTAA | 4 cut(s) | 120, 188, 630, 1076 |
| MslI | CAYNNNNRTG | 2 cut(s) | 206, 953 |
| Msp20I | TGGCCA | 1 cut(s) | 508 |
| MspR9I | CCNGG | 5 cut(s) | 30, 336, 391, 411, 792 |
| MvaI | CCWGG | 5 cut(s) | 30, 336, 391, 411, 792 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 24 |
| NarI | GGCGCC | 1 cut(s) | 738 |
| NcoI | CCATGG | 1 cut(s) | 509 |
| NdeI | CATATG | 1 cut(s) | 544 |
| NlaIII | CATG | 7 cut(s) | 513, 663, 911, 984, 1002, 1048, 1053 |
| NlaIV | GGNNCC | 3 cut(s) | 451, 515, 739 |
| NmuCI | GTSAC | 2 cut(s) | 574, 716 |
| NsiI | ATGCAT | 2 cut(s) | 544, 928 |
| NspI | RCATGY | 2 cut(s) | 911, 984 |
| NspV | TTCGAA | 1 cut(s) | 493 |
| OliI | CACNNNNGTG | 1 cut(s) | 206 |
| PaeI | GCATGC | 1 cut(s) | 984 |
| PciI | ACATGT | 1 cut(s) | 907 |
| PfeI | GAWTC | 3 cut(s) | 125, 355, 590 |
| PfoI | TCCNGGA | 1 cut(s) | 389 |
| PkrI | GCNGC | 1 cut(s) | 700 |
| PleI | GAGTC | 1 cut(s) | 1135 |
| PluTI | GGCGCC | 1 cut(s) | 741 |
| PpsI | GAGTC | 1 cut(s) | 1135 |
| PscI | ACATGT | 1 cut(s) | 907 |
| PshBI | ATTAAT | 1 cut(s) | 120 |
| PsiI | TTATAA | 1 cut(s) | 177 |
| Psp6I | CCWGG | 5 cut(s) | 28, 334, 389, 409, 790 |
| PspGI | CCWGG | 5 cut(s) | 28, 334, 389, 409, 790 |
| PspN4I | GGNNCC | 3 cut(s) | 451, 515, 739 |
| PspPI | GGNCC | 2 cut(s) | 198, 271 |
| RsaI | GTAC | 4 cut(s) | 232, 529, 870, 1135 |
| RsaNI | GTAC | 4 cut(s) | 231, 528, 869, 1134 |
| RseI | CAYNNNNRTG | 2 cut(s) | 206, 953 |
| SaqAI | TTAA | 4 cut(s) | 120, 188, 630, 1076 |
| SatI | GCNGC | 1 cut(s) | 699 |
| Sau96I | GGNCC | 2 cut(s) | 198, 271 |
| ScaI | AGTACT | 1 cut(s) | 232 |
| SchI | GAGTC | 1 cut(s) | 1136 |
| ScrFI | CCNGG | 5 cut(s) | 30, 336, 391, 411, 792 |
| SduI | GDGCHC | 1 cut(s) | 518 |
| SfaNI | GCATC | 4 cut(s) | 321, 913, 935, 1108 |
| SfoI | GGCGCC | 1 cut(s) | 739 |
| SfuI | TTCGAA | 1 cut(s) | 493 |
| SinI | GGWCC | 2 cut(s) | 198, 271 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 206, 953 |
| SpeI | ACTAGT | 1 cut(s) | 227 |
| SphI | GCATGC | 1 cut(s) | 984 |
| SspDI | GGCGCC | 1 cut(s) | 737 |
| SspMI | CTAG | 5 cut(s) | 14, 39, 228, 483, 1019 |
| StyD4I | CCNGG | 5 cut(s) | 28, 334, 389, 409, 790 |
| StyI | CCWWGG | 1 cut(s) | 509 |
| TaaI | ACNGT | 1 cut(s) | 284 |
| TaqI | TCGA | 3 cut(s) | 493, 1111, 1141 |
| TatI | WGTACW | 2 cut(s) | 230, 527 |
| TfiI | GAWTC | 3 cut(s) | 125, 355, 590 |
| Tru1I | TTAA | 4 cut(s) | 120, 188, 630, 1076 |
| Tru9I | TTAA | 4 cut(s) | 120, 188, 630, 1076 |
| TscAI | CASTG | 3 cut(s) | 93, 289, 751 |
| TseFI | GTSAC | 2 cut(s) | 574, 716 |
| TseI | GCWGC | 1 cut(s) | 698 |
| Tsp45I | GTSAC | 2 cut(s) | 574, 716 |
| TspDTI | ATGAA | 7 cut(s) | 17, 117, 356, 370, 417, 451, 987 |
| TspGWI | ACGGA | 2 cut(s) | 335, 741 |
| TspRI | CASTG | 3 cut(s) | 93, 289, 751 |
| VpaK11BI | GGWCC | 2 cut(s) | 198, 271 |
| VspI | ATTAAT | 1 cut(s) | 120 |
| XapI | RAATTY | 4 cut(s) | 57, 243, 612, 831 |
| XbaI | TCTAGA | 1 cut(s) | 1018 |
| XceI | RCATGY | 2 cut(s) | 911, 984 |
| XspI | CTAG | 5 cut(s) | 14, 39, 228, 483, 1019 |
| ZrmI | AGTACT | 1 cut(s) | 232 |
| Zsp2I | ATGCAT | 2 cut(s) | 544, 928 |