Rw5G028610

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000112
Physical Location & Seq
Reverse (-)
43346067 .. 43349427
3361 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G028610.1

Sequence Viewer

Length: 2586 bp
ATGAGTTATTGTCATCCCAGACTAGACAAAATGACCCTATTTGGAAAAGTTCAAAGTAGAAGAATTGCCCAGAAGATCAACATATTAATGCGACTCATCTCATTCATCACCAGCTCAAGTATGAAAATGAGAAAAAAGATGCAATTGGTTCATTCCTACAGAATTGAAAATGCTGAGAAAATGGTTTTGAAAGCTCTGCTTCTCTTTCTCTTCTTTCAGTTCTGCACTTCCAGGGACACCATGAGAGGGAACGAAGGACAATTGAAGGATGCTGATGGTGACTATTTAGTGTCCAAAGAAAGCAAGTTTGAATTGGGTTTCTTCAGCCCCGGAAATTCTAGCTACCGGTATGTTGGAATCTGGTATTCTCAGACTAGAGTACCTAATAAAACAGTGGTGTGGATTGCAAACAGGAACAATCCCATCAATGATACCTCTGGTGTGCTCACAATGAACAGGTATGGAGAACTAGTCCTTTATGCTTATAACATGGAGAGCAGCCCTATATGGTCCACAAATGCGTCACGGTCGGTTCAAAATGTTAACACAAGCACTTTATCTGCACAGCTTTTGGATACAGGAAATTTAGTTGTGTTCCAGGATGATAAAAATGAAATCTTTATATGGCAAAGTTTTGATTATCCTATAGATACTTTAATTCCAGGTATGAAAATTGGGGTGAATTGGAAAACTGGGCAAGAATGGGTTTTAACATCTTGGAAGTCACAAGATGACCCTGGAACTGGGGACTATACCTATAGGCTAAGTTCAGATCATAATGCATCTCCCCAATTTTTTTTGTATAAAGGTTTGAGTAAGTATTGGCGAAGTGATCCAGGGCCAGCGCCTACTTTGGTCGCTAATCAAGATGAAACTTATTATTTCATGAATGAAACCAATGCAATTGCAAGAATAACAGTGACTGATTCTGCCTTAGAGCGCCTTACATGGGATGGTGGTGGCCTTCAATGGAAGGAAGATTACTCTGCACCGAAGTCCCGTTGTGACAGGTACGGACAGTGTGGTGCCAACAGCAGATGTAGCCCTGATAATGTTAATCTGTTTGAGTGTGACTGTTTGCCAGGGTATGTGCCTAATTCTATAAGTGATTGGAATCAGAAAAATGGTTCAGGTGGATGTGTGAGTAATCGACTTGGTTTGTTGAAGTGTGGAGATGGAGACGGGTTTATAAAGGTGGCAAGAGTTAAATATCCAGACACATCGATAGCAGCATCATTAAAATCAGTGCCTAGAAATTGTTCTTGCACTGCATATTTGAGCGATGAAAATGAAGGGATTGTTGATTGCTTGACATGGTATGATGACTTGATGGACATTTTAGTGTACACAGAGATTGGAAGAGATCTCTATGTTCGTGTGAATGCAACTGTCTTAGCGGCAAATGCAGGAAGATCACAAGGTTTGTGGGAAAGGAGGGGTATGCTGGCTATTCCAATACTGTGCAGTGCGCTAGCATTGGTACTAATCATTATGCTTGCATGGAAGTGGCGTAAGAAGAACAGGAACACAAGAGGCATAGACTTTGTGGAGGCAGAGGAGCTTGAGGAAACTCAGAGACACCCCGAATTGCAATTTTTCGATCTTGACACAATAACAGCAGCCACAGACCACTTCTCTCATGTCAATGAACTTGGCCGCGGTGGCTTTGGCTCTGTTTATATGGGTCAGCTATCAAATGAACAGAAAGTTGCTGTGAAAAGATTGTCCAAAACTTCAGGACAAGGGATTGAAGAATTTAAGAATGAAGTTGCACTTATAGCAAGACTTCAACATAGGAACCTTGTGAAACTTTTAGGCTGTTGTATAAAGGGAGAAGAAAGGATGTTAGTCCTAGAATACATGCCTAACAAAGGCTTGGACTCCTTTCTTTTTGATAAGACAAGACGGTCCTTCTTGGATTGGGAAAAGCGTTTTGAAATTATCAACGGGATTGCTCGTGGGATTCTATATCTTCACCAAGACTCAAGATTGAGGATTATCCATAGAGATCTAAAAACTAGTAATGTTCTACTAGATGATGAGATGAACCCAAAAATTTCTGATTTTGGTATGGCTAGAATATTCCATGGAGACCAACTGCAAGATAAGACAAGCCGAATTGTCGGAACATATGGCTACATGTCACCGGAGTACGCAGTATTTGGGAGATTTTCCACAAAGTCTGATGTCTTTAGTTTTGGGATCATAATGTTGGAGATTGTAAGTGGCCAGAAAAACAATGGTTCTGATCCGGAGGATCCTTCCGTGAACTTAATAGGACGTGTATGGGAGCTCTGGAGAGAAGGCAGAGCCTTGGATATTGTGGATTCAACACTGAAGTCATATCATCCAAATGAAGCCATGAGATGCATACAAGTTGGTCTCTTGTGTGTACAAGAAGATCCGATGGACCGACCTGTCATGTCAGCCATTGTTTTCATGTTGAGTGATGAAGCATCTCCTCCATTGCCAAAGCAGCCGCCAATTGTTTACAGAAGAAATTTCGGCACTGATGTTGATCCATTACTTTCAAATAGATTTTTTTCTAGAAATGACTTGACAGTAACTACAATGGAAGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

861

Amino Acids

97.83

Weight (kDa)

6.85

Isoelectric Point (pI)

36.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 131 - 241 3.9e-35 D-mannose binding lectin
S_locus_glycop PF00954 290 - 366 1.1e-12 S-locus glycoprotein domain
PAN_2 PF08276 390 - 446 7.7e-07 PAN-like domain
Pkinase PF00069 546 - 808 2.3e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 548 - 813 1.9e-50 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 486, 1190
AasI GACNNNNNNGTC 2 cut(s) 1906, 2417
AccB1I GGYRCC 1 cut(s) 1025
AccII CGCG 1 cut(s) 1659
AccIII TCCGGA 1 cut(s) 2251
AciI CCGC 4 cut(s) 1397, 1657, 1659, 2480
AclWI GGATC 7 cut(s) 827, 2210, 2243, 2252, 2265, 2396, 2513
AcoI YGGCCR 2 cut(s) 1654, 2227
AcsI RAATTY 5 cut(s) 334, 583, 1754, 2055, 2500
AcuI CTGAAG 3 cut(s) 307, 1719, 2357
AfaI GTAC 6 cut(s) 381, 1013, 1346, 1482, 2153, 2394
AfiI CCNNNNNNNGG 4 cut(s) 246, 743, 949, 1871
AflIII ACRYGT 2 cut(s) 2139, 2281
AgeI ACCGGT 1 cut(s) 345
AhlI ACTAGT 2 cut(s) 469, 2018
AjiI CACGTC 1 cut(s) 2282
AjnI CCWGG 6 cut(s) 230, 597, 661, 736, 835, 1081
AluBI AGCT 8 cut(s) 114, 194, 342, 568, 1561, 1690, 2293, 2579
AluI AGCT 8 cut(s) 114, 194, 342, 568, 1561, 1690, 2293, 2579
Alw21I GWGCWC 2 cut(s) 447, 2295
Alw26I GTCTC 4 cut(s) 1173, 1570, 2085, 2387
AlwI GGATC 7 cut(s) 827, 2210, 2243, 2252, 2265, 2396, 2513
AlwNI CAGNNNCTG 1 cut(s) 923
Aor13HI TCCGGA 1 cut(s) 2251
AoxI GGCC 4 cut(s) 839, 961, 1654, 2227
ApeKI GCWGC 4 cut(s) 498, 1229, 1619, 2476
ApoI RAATTY 5 cut(s) 334, 583, 1754, 2055, 2500
AseI ATTAAT 1 cut(s) 86
AsiGI ACCGGT 1 cut(s) 345
Asp700I GAANNNNTTC 1 cut(s) 1258
AspLEI GCGC 3 cut(s) 847, 942, 1471
AspS9I GGNCC 4 cut(s) 510, 839, 1908, 2410
AsuC2I CCSGG 1 cut(s) 330
AsuHPI GGTGA 5 cut(s) 100, 290, 691, 1967, 2136
AsuNHI GCTAGC 1 cut(s) 1471
AvaII GGWCC 3 cut(s) 510, 1908, 2410
BaeI ACNNNNGTAYC 2 cut(s) 363, 396
BalI TGGCCA 1 cut(s) 2229
BamHI GGATCC 1 cut(s) 2257
BanI GGYRCC 1 cut(s) 1025
BanII GRGCYC 1 cut(s) 2295
BarI GAAGNNNNNNTAC 2 cut(s) 965, 997
BauI CACGAG 1 cut(s) 1956
Bbv12I GWGCWC 2 cut(s) 447, 2295
BbvI GCAGC 4 cut(s) 510, 1241, 1631, 2488
BccI CCATC 6 cut(s) 269, 431, 947, 1169, 1324, 2401
BciT130I CCWGG 6 cut(s) 232, 599, 663, 738, 837, 1083
BciVI GTATCC 1 cut(s) 568
BcnI CCSGG 1 cut(s) 330
BcoDI GTCTC 4 cut(s) 1173, 1570, 2085, 2387
BcuI ACTAGT 2 cut(s) 469, 2018
BfmI CTRYAG 3 cut(s) 157, 645, 757
BfoI RGCGCY 2 cut(s) 848, 943
BfuI GTATCC 1 cut(s) 568
BglI GCCNNNNNGGC 1 cut(s) 1662
BglII AGATCT 2 cut(s) 1363, 2008
BisI GCNGC 7 cut(s) 499, 1230, 1398, 1620, 1657, 2477, 2480
BlsI GCNGC 7 cut(s) 500, 1231, 1399, 1621, 1658, 2478, 2481
Bme1390I CCNGG 7 cut(s) 232, 330, 599, 663, 738, 837, 1083
Bme18I GGWCC 3 cut(s) 510, 1908, 2410
BmgBI CACGTC 1 cut(s) 2282
BmgT120I GGNCC 4 cut(s) 510, 839, 1908, 2410
BmiI GGNNCC 3 cut(s) 1027, 1799, 2259
BmrFI CCNGG 7 cut(s) 232, 330, 599, 663, 738, 837, 1083
BmrI ACTGGG 2 cut(s) 702, 753
BmsI GCATC 6 cut(s) 129, 259, 791, 1241, 2357, 2465
BmtI GCTAGC 1 cut(s) 1475
BmuI ACTGGG 2 cut(s) 702, 753
BpmI CTGGAG 1 cut(s) 2317
BpuEI CTTGAG 3 cut(s) 100, 1583, 1969
BpuMI CCSGG 1 cut(s) 330
Bsa29I ATCGAT 1 cut(s) 1223
BsaBI GATNNNNATC 2 cut(s) 1113, 2517
BsaI GGTCTC 2 cut(s) 2085, 2387
BsaJI CCNNGG 8 cut(s) 231, 328, 736, 836, 1082, 1657, 2086, 2313
BsaWI WCCGGW 3 cut(s) 345, 2146, 2251
BsaXI ACNNNNNCTCC 2 cut(s) 456, 486
Bsc4I CCNNNNNNNGG 4 cut(s) 246, 743, 949, 1871
Bse118I RCCGGY 1 cut(s) 345
Bse1I ACTGG 2 cut(s) 697, 748
Bse3DI GCAATG 1 cut(s) 2465
Bse8I GATNNNNATC 2 cut(s) 1113, 2517
BseAI TCCGGA 1 cut(s) 2251
BseBI CCWGG 6 cut(s) 232, 599, 663, 738, 837, 1083
BseCI ATCGAT 1 cut(s) 1223
BseDI CCNNGG 8 cut(s) 231, 328, 736, 836, 1082, 1657, 2086, 2313
BseGI GGATG 7 cut(s) 13, 274, 607, 958, 1142, 1848, 2347
BseJI GATNNNNATC 2 cut(s) 1113, 2517
BseLI CCNNNNNNNGG 4 cut(s) 246, 743, 949, 1871
BseMI GCAATG 1 cut(s) 2465
BseMII CTCAG 3 cut(s) 165, 383, 1586
BseNI ACTGG 2 cut(s) 697, 748
BseRI GAGGAG 2 cut(s) 1571, 2451
BseXI GCAGC 4 cut(s) 510, 1241, 1631, 2488
BsgI GTGCAG 4 cut(s) 208, 546, 972, 1483
Bsh1236I CGCG 1 cut(s) 1659
Bsh1285I CGRYCG 1 cut(s) 530
BshFI GGCC 4 cut(s) 841, 963, 1656, 2229
BshNI GGYRCC 1 cut(s) 1025
BshTI ACCGGT 1 cut(s) 345
BshVI ATCGAT 1 cut(s) 1223
BsiEI CGRYCG 1 cut(s) 530
BsiHKAI GWGCWC 2 cut(s) 447, 2295
BsiSI CCGG 4 cut(s) 330, 346, 2147, 2252
BslFI GGGAC 3 cut(s) 248, 761, 982
BslI CCNNNNNNNGG 4 cut(s) 246, 743, 949, 1871
BsmAI GTCTC 4 cut(s) 1173, 1570, 2085, 2387
BsmBI CGTCTC 1 cut(s) 1173
BsmFI GGGAC 3 cut(s) 248, 761, 982
BsmI GAATGC 1 cut(s) 1387
BsnI GGCC 4 cut(s) 841, 963, 1656, 2229
Bso31I GGTCTC 2 cut(s) 2085, 2387
Bsp1286I GDGCHC 2 cut(s) 447, 2295
Bsp13I TCCGGA 1 cut(s) 2251
Bsp1407I TGTACA 2 cut(s) 1344, 2392
Bsp19I CCATGG 1 cut(s) 2086
BspACI CCGC 4 cut(s) 1397, 1657, 1659, 2480
BspANI GGCC 4 cut(s) 841, 963, 1656, 2229
BspCNI CTCAG 3 cut(s) 166, 382, 1585
BspDI ATCGAT 1 cut(s) 1223
BspEI TCCGGA 1 cut(s) 2251
BspFNI CGCG 1 cut(s) 1659
BspHI TCATGA 1 cut(s) 885
BspLI GGNNCC 3 cut(s) 1027, 1799, 2259
BspOI GCTAGC 1 cut(s) 1475
BspPI GGATC 7 cut(s) 827, 2210, 2243, 2252, 2265, 2396, 2513
BspT107I GGYRCC 1 cut(s) 1025
BspTNI GGTCTC 2 cut(s) 2085, 2387
BsrDI GCAATG 1 cut(s) 2465
BsrFI RCCGGY 1 cut(s) 345
BsrGI TGTACA 2 cut(s) 1344, 2392
BsrI ACTGG 2 cut(s) 697, 748
BssAI RCCGGY 1 cut(s) 345
BssECI CCNNGG 8 cut(s) 231, 328, 736, 836, 1082, 1657, 2086, 2313
BssSI CACGAG 1 cut(s) 1956
BssT1I CCWWGG 2 cut(s) 2086, 2313
Bst2BI CACGAG 1 cut(s) 1956
Bst2UI CCWGG 6 cut(s) 232, 599, 663, 738, 837, 1083
Bst4CI ACNGT 9 cut(s) 394, 528, 919, 1020, 1076, 1390, 1461, 1908, 2563
Bst6I CTCTTC 2 cut(s) 215, 1354
BstAUI TGTACA 2 cut(s) 1344, 2392
BstC8I GCNNGC 4 cut(s) 843, 1446, 1473, 1497
BstDEI CTNAG 6 cut(s) 174, 369, 764, 934, 1393, 1572
BstDSI CCRYGG 2 cut(s) 1657, 2086
BstENI CCTNNNNNAGG 1 cut(s) 1869
BstF5I GGATG 7 cut(s) 13, 274, 607, 958, 1142, 1848, 2347
BstFNI CGCG 1 cut(s) 1659
BstH2I RGCGCY 2 cut(s) 848, 943
BstHHI GCGC 3 cut(s) 847, 942, 1471
BstMAI GTCTC 4 cut(s) 1173, 1570, 2085, 2387
BstMCI CGRYCG 1 cut(s) 530
BstMWI GCNNNNNNNGC 5 cut(s) 1041, 1403, 1662, 1778, 2476
BstNI CCWGG 6 cut(s) 232, 599, 663, 738, 837, 1083
BstNSI RCATGY 2 cut(s) 1864, 2143
BstSCI CCNGG 7 cut(s) 230, 328, 597, 661, 736, 835, 1081
BstSFI CTRYAG 3 cut(s) 157, 645, 757
BstUI CGCG 1 cut(s) 1659
BstV1I GCAGC 4 cut(s) 510, 1241, 1631, 2488
BstX2I RGATCY 4 cut(s) 1363, 2008, 2257, 2401
BstYI RGATCY 4 cut(s) 1363, 2008, 2257, 2401
Bsu15I ATCGAT 1 cut(s) 1223
BsuI GTATCC 1 cut(s) 568
BsuRI GGCC 4 cut(s) 841, 963, 1656, 2229
BsuTUI ATCGAT 1 cut(s) 1223
BtgI CCRYGG 2 cut(s) 1657, 2086
BtgZI GCGATG 1 cut(s) 1296
BtrI CACGTC 1 cut(s) 2282
BtsCI GGATG 7 cut(s) 13, 274, 607, 958, 1142, 1848, 2347
BtsI GCAGTG 2 cut(s) 1266, 1471
BtsIMutI CAGTG 8 cut(s) 399, 924, 1025, 1251, 1266, 1471, 2333, 2508
Cac8I GCNNGC 4 cut(s) 843, 1446, 1473, 1497
CaiI CAGNNNCTG 1 cut(s) 923
CciI TCATGA 1 cut(s) 885
CfoI GCGC 3 cut(s) 847, 942, 1471
Cfr10I RCCGGY 1 cut(s) 345
Cfr13I GGNCC 4 cut(s) 510, 839, 1908, 2410
Cfr42I CCGCGG 1 cut(s) 1660
ClaI ATCGAT 1 cut(s) 1223
CseI GACGC 1 cut(s) 510
Csp6I GTAC 6 cut(s) 380, 1012, 1345, 1481, 2152, 2393
CspAI ACCGGT 1 cut(s) 345
CspCI CAANNNNNGTGG 2 cut(s) 1406, 1441
CviQI GTAC 6 cut(s) 380, 1012, 1345, 1481, 2152, 2393
DdeI CTNAG 6 cut(s) 174, 369, 764, 934, 1393, 1572
DrdI GACNNNNNNGTC 2 cut(s) 1906, 2417
DseDI GACNNNNNNGTC 2 cut(s) 1906, 2417
EaeI YGGCCR 2 cut(s) 1654, 2227
Eam1104I CTCTTC 2 cut(s) 215, 1354
EarI CTCTTC 2 cut(s) 215, 1354
Ecl136II GAGCTC 1 cut(s) 2293
Eco130I CCWWGG 2 cut(s) 2086, 2313
Eco24I GRGCYC 1 cut(s) 2295
Eco31I GGTCTC 2 cut(s) 2085, 2387
Eco47I GGWCC 3 cut(s) 510, 1908, 2410
Eco53kI GAGCTC 1 cut(s) 2293
Eco57I CTGAAG 3 cut(s) 307, 1719, 2357
EcoICRI GAGCTC 1 cut(s) 2293
EcoNI CCTNNNNNAGG 1 cut(s) 1869
EcoRII CCWGG 6 cut(s) 230, 597, 661, 736, 835, 1081
EcoT14I CCWWGG 2 cut(s) 2086, 2313
EcoT22I ATGCAT 2 cut(s) 784, 2372
EcoT38I GRGCYC 1 cut(s) 2295
ErhI CCWWGG 2 cut(s) 2086, 2313
Esp3I CGTCTC 1 cut(s) 1173
FalI AAGNNNNNCTT 4 cut(s) 183, 215, 1758, 1790
FaqI GGGAC 3 cut(s) 248, 761, 982
FauNDI CATATG 1 cut(s) 2131
Fnu4HI GCNGC 7 cut(s) 499, 1230, 1398, 1620, 1657, 2477, 2480
FokI GGATG 6 cut(s) 281, 614, 965, 1149, 1855, 2334
FriOI GRGCYC 1 cut(s) 2295
Fsp4HI GCNGC 7 cut(s) 499, 1230, 1398, 1620, 1657, 2477, 2480
GlaI GCGC 3 cut(s) 846, 941, 1470
GluI GCNGC 7 cut(s) 499, 1230, 1398, 1620, 1657, 2477, 2480
GsuI CTGGAG 1 cut(s) 2317
HaeII RGCGCY 2 cut(s) 848, 943
HaeIII GGCC 4 cut(s) 841, 963, 1656, 2229
HapII CCGG 4 cut(s) 330, 346, 2147, 2252
HgaI GACGC 1 cut(s) 510
HhaI GCGC 3 cut(s) 847, 942, 1471
Hin6I GCGC 3 cut(s) 845, 940, 1469
HinP1I GCGC 3 cut(s) 845, 940, 1469
HincII GTYRAC 1 cut(s) 544
HindII GTYRAC 1 cut(s) 544
HinfI GANTC 8 cut(s) 93, 357, 926, 1114, 1880, 1963, 1982, 2327
HpaI GTTAAC 1 cut(s) 544
HpaII CCGG 4 cut(s) 330, 346, 2147, 2252
HphI GGTGA 5 cut(s) 100, 290, 691, 1967, 2136
Hpy166II GTNNAC 7 cut(s) 513, 544, 1345, 1347, 2269, 2393, 2491
Hpy188I TCNGA 9 cut(s) 372, 772, 1119, 1575, 2062, 2126, 2185, 2248, 2406
Hpy188III TCNNGA 9 cut(s) 866, 886, 1214, 1604, 1737, 1986, 2252, 2296, 2547
Hpy8I GTNNAC 7 cut(s) 513, 544, 1345, 1347, 2269, 2393, 2491
HpyAV CCTTC 8 cut(s) 248, 259, 967, 974, 1286, 1921, 2271, 2297
HpyCH4III ACNGT 9 cut(s) 394, 528, 919, 1020, 1076, 1390, 1461, 1908, 2563
HpyCH4IV ACGT 1 cut(s) 2281
HpyF10VI GCNNNNNNNGC 5 cut(s) 1041, 1403, 1662, 1778, 2476
HpyF3I CTNAG 6 cut(s) 174, 369, 764, 934, 1393, 1572
HpySE526I ACGT 1 cut(s) 2281
HspAI GCGC 3 cut(s) 845, 940, 1469
Kpn2I TCCGGA 1 cut(s) 2251
KspAI GTTAAC 1 cut(s) 544
KspI CCGCGG 1 cut(s) 1660
LmnI GCTCC 2 cut(s) 1558, 2290
Lsp1109I GCAGC 4 cut(s) 510, 1241, 1631, 2488
LweI GCATC 6 cut(s) 129, 259, 791, 1241, 2357, 2465
MaeII ACGT 1 cut(s) 2281
MaeIII GTNAC 8 cut(s) 278, 522, 723, 919, 1004, 1070, 2142, 2563
MfeI CAATTG 4 cut(s) 143, 260, 903, 2484
MflI RGATCY 4 cut(s) 1363, 2008, 2257, 2401
MhlI GDGCHC 2 cut(s) 447, 2295
MlsI TGGCCA 1 cut(s) 2229
MluNI TGGCCA 1 cut(s) 2229
MlyI GAGTC 3 cut(s) 87, 1874, 1976
MmeI TCCRAC 3 cut(s) 334, 2104, 2193
Mox20I TGGCCA 1 cut(s) 2229
Mph1103I ATGCAT 2 cut(s) 784, 2372
MroI TCCGGA 1 cut(s) 2251
MroXI GAANNNNTTC 1 cut(s) 1258
MscI TGGCCA 1 cut(s) 2229
MseI TTAA 9 cut(s) 86, 543, 656, 710, 1056, 1206, 1238, 1758, 2273
MslI CAYNNNNRTG 5 cut(s) 86, 1340, 1504, 1644, 2352
Msp20I TGGCCA 1 cut(s) 2229
MspA1I CMGCKG 1 cut(s) 1659
MspI CCGG 4 cut(s) 330, 346, 2147, 2252
MspR9I CCNGG 7 cut(s) 232, 330, 599, 663, 738, 837, 1083
MunI CAATTG 4 cut(s) 143, 260, 903, 2484
Mva1269I GAATGC 1 cut(s) 1387
MvaI CCWGG 6 cut(s) 232, 599, 663, 738, 837, 1083
MvnI CGCG 1 cut(s) 1659
MwoI GCNNNNNNNGC 5 cut(s) 1041, 1403, 1662, 1778, 2476
NciI CCSGG 1 cut(s) 330
NcoI CCATGG 1 cut(s) 2086
NdeI CATATG 1 cut(s) 2131
NheI GCTAGC 1 cut(s) 1471
NlaIV GGNNCC 3 cut(s) 1027, 1799, 2259
NmuCI GTSAC 7 cut(s) 278, 522, 723, 919, 1004, 1070, 2142
NsiI ATGCAT 2 cut(s) 784, 2372
NspI RCATGY 2 cut(s) 1864, 2143
PagI TCATGA 1 cut(s) 885
PciI ACATGT 1 cut(s) 2139
PctI GAATGC 1 cut(s) 1387
PdmI GAANNNNTTC 1 cut(s) 1258
PfeI GAWTC 5 cut(s) 357, 926, 1114, 1963, 2327
PfoI TCCNGGA 1 cut(s) 597
PinAI ACCGGT 1 cut(s) 345
PkrI GCNGC 7 cut(s) 500, 1231, 1399, 1621, 1658, 2478, 2481
PleI GAGTC 3 cut(s) 87, 1874, 1976
PpsI GAGTC 3 cut(s) 87, 1874, 1976
PscI ACATGT 1 cut(s) 2139
PshBI ATTAAT 1 cut(s) 86
PsiI TTATAA 2 cut(s) 486, 1190
Psp124BI GAGCTC 1 cut(s) 2295
Psp6I CCWGG 6 cut(s) 230, 597, 661, 736, 835, 1081
PspGI CCWGG 6 cut(s) 230, 597, 661, 736, 835, 1081
PspN4I GGNNCC 3 cut(s) 1027, 1799, 2259
PspPI GGNCC 4 cut(s) 510, 839, 1908, 2410
PstNI CAGNNNCTG 1 cut(s) 923
PsuI RGATCY 4 cut(s) 1363, 2008, 2257, 2401
RsaI GTAC 6 cut(s) 381, 1013, 1346, 1482, 2153, 2394
RsaNI GTAC 6 cut(s) 380, 1012, 1345, 1481, 2152, 2393
RseI CAYNNNNRTG 5 cut(s) 86, 1340, 1504, 1644, 2352
SacI GAGCTC 1 cut(s) 2295
SacII CCGCGG 1 cut(s) 1660
SaqAI TTAA 9 cut(s) 86, 543, 656, 710, 1056, 1206, 1238, 1758, 2273
SatI GCNGC 7 cut(s) 499, 1230, 1398, 1620, 1657, 2477, 2480
Sau96I GGNCC 4 cut(s) 510, 839, 1908, 2410
SchI GAGTC 3 cut(s) 87, 1874, 1976
ScrFI CCNGG 7 cut(s) 232, 330, 599, 663, 738, 837, 1083
SduI GDGCHC 2 cut(s) 447, 2295
SfaNI GCATC 6 cut(s) 129, 259, 791, 1241, 2357, 2465
SfcI CTRYAG 3 cut(s) 157, 645, 757
Sfr303I CCGCGG 1 cut(s) 1660
SgrBI CCGCGG 1 cut(s) 1660
SinI GGWCC 3 cut(s) 510, 1908, 2410
SmiMI CAYNNNNRTG 5 cut(s) 86, 1340, 1504, 1644, 2352
SmlI CTYRAG 3 cut(s) 115, 1562, 1984
SmoI CTYRAG 3 cut(s) 115, 1562, 1984
SpeI ACTAGT 2 cut(s) 469, 2018
SsiI CCGC 4 cut(s) 1397, 1657, 1659, 2480
SspI AATATT 1 cut(s) 2082
SstI GAGCTC 1 cut(s) 2295
StyD4I CCNGG 7 cut(s) 230, 328, 597, 661, 736, 835, 1081
StyI CCWWGG 2 cut(s) 2086, 2313
TaaI ACNGT 9 cut(s) 394, 528, 919, 1020, 1076, 1390, 1461, 1908, 2563
TaiI ACGT 1 cut(s) 2284
TaqI TCGA 4 cut(s) 1150, 1223, 1599, 2581
TaqII GACCGA 1 cut(s) 2427
TatI WGTACW 2 cut(s) 1344, 2392
TauI GCSGC 3 cut(s) 1400, 1659, 2482
TfiI GAWTC 5 cut(s) 357, 926, 1114, 1963, 2327
Tru1I TTAA 9 cut(s) 86, 543, 656, 710, 1056, 1206, 1238, 1758, 2273
Tru9I TTAA 9 cut(s) 86, 543, 656, 710, 1056, 1206, 1238, 1758, 2273
TscAI CASTG 8 cut(s) 399, 924, 1025, 1251, 1273, 1471, 2340, 2515
TseFI GTSAC 7 cut(s) 278, 522, 723, 919, 1004, 1070, 2142
TseI GCWGC 4 cut(s) 498, 1229, 1619, 2476
Tsp45I GTSAC 7 cut(s) 278, 522, 723, 919, 1004, 1070, 2142
TspGWI ACGGA 2 cut(s) 1029, 2254
TspRI CASTG 8 cut(s) 399, 924, 1025, 1251, 1273, 1471, 2340, 2515
VpaK11BI GGWCC 3 cut(s) 510, 1908, 2410
VspI ATTAAT 1 cut(s) 86
XagI CCTNNNNNAGG 1 cut(s) 1869
XapI RAATTY 5 cut(s) 334, 583, 1754, 2055, 2500
XbaI TCTAGA 1 cut(s) 2546
XceI RCATGY 2 cut(s) 1864, 2143
XcmI CCANNNNNNNNNTGG 1 cut(s) 2237
XmnI GAANNNNTTC 1 cut(s) 1258
Zsp2I ATGCAT 2 cut(s) 784, 2372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.