Rmu_sc0013603.1_g000006

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0013603.1
OG0000112
Physical Location & Seq
Reverse (-)
38061 .. 41315
3255 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0013603.1_g000006.1.cds

Sequence Viewer

Length: 2478 bp
atgccactgtttgctacctacaaagttgctaatgcccagaaaatgttggtcaaggctctgcttctcttcctcctcttccgattttgcgattccagggacaccttaacatggaaccaagaacaactgaaggatgatggtggagttttagtgtccaaacagagcaagttcgagttgggtttcttcagccccggaaattctagctaccggtatattggaatttggtattctcagactaaagtatctgagaaaacagtggtgtgggttgcaaacaggaacaatcccatcaatgatacctctggtgtgctcaaaataaacaggtatggagaactggccctttattcttataacatgaacagcactcctgtttggtccactaatgtgtcacggccggtccaaaataataacgcaagcactttttctgcacagcttttagatgcaggaaatttagttgtgttccaggatgataataatgaaatctatgcatggcaaagttttgattatcctacagatactctaattccaggtatgaaagtcggggtgaattggaaaactgggctagaatgggttttaacatcttggaagtcacaagatgaccctggacttggggactattcctttaggctctatccagatcagactgccaccccccaatattttatgtataaaggtttgagcaagtattggcgaagtgagtcagggccagggcctccttttgtcactaatcaagaggaaacttatcatttcttcaattacaccaatgcaattacaagaacgacgttgaacgattctggtaggttatatcgccttacatgggataatggtggcgttcaatggaaggaagatttttctgcaccgaagtcccggtgtgactggtatggacagtgtggtgccaacagcaaatgtaagcctgacaatattaatgagtttgagtgtgaatgtttaccagggtatgagcctagttctataaatgattggaatcagaaaaatgggtcgggtggatgtgtgagtaagcgagttggtgtatcaaagtgcgggaatggagatgggtttgtgaatgtggcaagagttaaatttccagacacatcaatagcatcacggttgagaacaggtatgagtgccaaagagtgtgagcaggactgcttaagaaattgttcttgcaccgcatatttgagcatcgaatatgaagggcttgtggattgcttgacatggtatgatgacttgatggatattttagtgtacacagagcgtggacgagatttatacgtacgtctaaataaaacagagttagctgcatatgcaggaagatcacaagttttcttggaaaggaggggtatgctcgctattccaatattgtctgctctactggcattggtcctaatcattatgcttgcttgttggtggagtaagaagaacaggaacacaaaaggcatagtctttgtggaggcagaggagcttgaggaaactcagagacaccccgaattgcaatttttcgatcttgacacaataatagcagccacagacgacttctctcgtgtcaatgaacttggccatggtggttttggctctgtttataagggtcagctaccaaatgaacagaaagttgctgtgaaaagattgtccaaaacttcaggacaagggattgaagaattcaaaaatgaagttgcacttatagcaagacttcaacacaggaaccttgtgaaacttttaggctgttgtataaagggagaagaaaggatattagtcctagaatacatgcctaacaaaagcttggactcctttctttttgataacacaagaaggtccttcttggattgggaaaagcgctttgaaattatcaatgggattgctcgtgggattctatatcttcaccaagactcaagattgaggattatccatagagatctaaaaactagcaatgttctactagatgctgagatgaacccaaaaatttctgattttggcatggctagaatattccacggggaccaactgcaagataagacgaaccgaattgtcggaacatatggctacatgtcaccggagtacgcggtatttggaagattttccacaaaatctgatgtctttagttttgggatcataatgttggagattttaagtggccagaaaaacaatggttttgatctagaagatccttccatgaacttaataggacgtgtttgggagctctgggaagaaggcatagccttagatattttggattcgacattgaagtcatatcagcctaatgaagtcatgagatgcatacaagttgcgctcttgtgtgtacaagaagattcgaaggaccgacctgccatgtcagccgttgttttcatgttgagtggtgaagcatctcctccattgcctaagcagccagcgtttgtttacagaagaaattccggcactgatgtttatccattactttcaaagagatcttattccataaacgatgtgacagtatctacaatggaagctcgataa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

825

Amino Acids

93.75

Weight (kDa)

5.91

Isoelectric Point (pI)

41.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 345, 1572
AasI GACNNNNNNGTC 1 cut(s) 2230
Acc36I ACCTGC 1 cut(s) 2317
AccB1I GGYRCC 1 cut(s) 887
AccII CGCG 1 cut(s) 2048
AciI CCGC 3 cut(s) 1032, 1161, 2048
AclWI GGATC 2 cut(s) 2102, 2144
AcoI YGGCCR 3 cut(s) 386, 1546, 2119
AcsI RAATTY 7 cut(s) 193, 216, 442, 1070, 1646, 1947, 2392
AcuI CTGAAG 3 cut(s) 146, 166, 1611
AfaI GTAC 4 cut(s) 1238, 1266, 2045, 2286
AfeI AGCGCT 1 cut(s) 1823
AfiI CCNNNNNNNGG 3 cut(s) 108, 602, 811
AflII CTTAAG 1 cut(s) 1141
AflIII ACRYGT 2 cut(s) 2031, 2173
AgeI ACCGGT 1 cut(s) 204
AgsI TTSAA 9 cut(s) 748, 781, 830, 1643, 1651, 1682, 1829, 2230, 2424
AjiI CACGTC 1 cut(s) 2174
AjnI CCWGG 6 cut(s) 92, 456, 520, 595, 700, 943
AleI CACNNNNGTG 1 cut(s) 377
AluBI AGCT 8 cut(s) 201, 427, 1289, 1453, 1582, 1767, 2185, 2471
AluI AGCT 8 cut(s) 201, 427, 1289, 1453, 1582, 1767, 2185, 2471
Alw21I GWGCWC 2 cut(s) 306, 2187
Alw26I GTCTC 1 cut(s) 1462
AlwI GGATC 2 cut(s) 2102, 2144
Aor51HI AGCGCT 1 cut(s) 1823
AoxI GGCC 6 cut(s) 330, 386, 698, 704, 1546, 2119
ApeKI GCWGC 3 cut(s) 1289, 1511, 2368
ApoI RAATTY 7 cut(s) 193, 216, 442, 1070, 1646, 1947, 2392
AseI ATTAAT 1 cut(s) 918
AsiGI ACCGGT 1 cut(s) 204
Asp700I GAANNNNTTC 3 cut(s) 1150, 2062, 2392
AspLEI GCGC 2 cut(s) 1824, 2275
AspS9I GGNCC 9 cut(s) 331, 369, 391, 698, 704, 1372, 1800, 1984, 2302
AsuC2I CCSGG 2 cut(s) 189, 862
AsuHPI GGTGA 4 cut(s) 550, 1859, 2028, 2354
AsuII TTCGAA 1 cut(s) 2297
AvaII GGWCC 6 cut(s) 369, 391, 1372, 1800, 1984, 2302
BaeI ACNNNNGTAYC 2 cut(s) 222, 255
BalI TGGCCA 2 cut(s) 1548, 2121
BanI GGYRCC 1 cut(s) 887
BanII GRGCYC 1 cut(s) 2187
BauI CACGAG 2 cut(s) 1530, 1848
Bbv12I GWGCWC 2 cut(s) 306, 2187
BbvI GCAGC 3 cut(s) 1276, 1523, 2380
BccI CCATC 4 cut(s) 128, 290, 1037, 1216
BceAI ACGGC 2 cut(s) 401, 2306
BciT130I CCWGG 6 cut(s) 94, 458, 522, 597, 702, 945
BcnI CCSGG 2 cut(s) 189, 862
BcoDI GTCTC 1 cut(s) 1462
BfaI CTAG 8 cut(s) 198, 557, 957, 1745, 1911, 1925, 1968, 2144
BfmI CTRYAG 1 cut(s) 504
BfoI RGCGCY 1 cut(s) 1825
BfrI CTTAAG 1 cut(s) 1141
BfuAI ACCTGC 1 cut(s) 2317
BglII AGATCT 2 cut(s) 1900, 2429
BisI GCNGC 3 cut(s) 1290, 1512, 2369
BlsI GCNGC 3 cut(s) 1291, 1513, 2370
Bme1390I CCNGG 8 cut(s) 94, 189, 458, 522, 597, 702, 862, 945
Bme18I GGWCC 6 cut(s) 369, 391, 1372, 1800, 1984, 2302
BmgBI CACGTC 1 cut(s) 2174
BmgT120I GGNCC 9 cut(s) 331, 369, 391, 698, 704, 1372, 1800, 1984, 2302
BmiI GGNNCC 4 cut(s) 113, 889, 1691, 1985
BmrFI CCNGG 8 cut(s) 94, 189, 458, 522, 597, 702, 862, 945
BmrI ACTGGG 1 cut(s) 561
BmsI GCATC 6 cut(s) 424, 1100, 1182, 1918, 2249, 2357
BmuI ACTGGG 1 cut(s) 561
Bpu10I CCTNAGC 1 cut(s) 2364
Bpu14I TTCGAA 1 cut(s) 2297
BpuEI CTTGAG 2 cut(s) 1475, 1861
BpuMI CCSGG 2 cut(s) 189, 862
BsaAI YACGTR 1 cut(s) 1264
BsaBI GATNNNNATC 2 cut(s) 975, 2409
BsaJI CCNNGG 7 cut(s) 93, 187, 595, 701, 944, 1549, 1978
BsaWI WCCGGW 2 cut(s) 204, 2038
BsaXI ACNNNNNCTCC 2 cut(s) 132, 162
Bsc4I CCNNNNNNNGG 3 cut(s) 108, 602, 811
Bse118I RCCGGY 2 cut(s) 204, 388
Bse1I ACTGG 4 cut(s) 333, 556, 875, 1368
Bse3DI GCAATG 2 cut(s) 1921, 2357
Bse8I GATNNNNATC 2 cut(s) 975, 2409
BseBI CCWGG 6 cut(s) 94, 458, 522, 597, 702, 945
BseDI CCNNGG 7 cut(s) 93, 187, 595, 701, 944, 1549, 1978
BseGI GGATG 3 cut(s) 136, 466, 1004
BseJI GATNNNNATC 2 cut(s) 975, 2409
BseLI CCNNNNNNNGG 3 cut(s) 108, 602, 811
BseMI GCAATG 2 cut(s) 1921, 2357
BseMII CTCAG 4 cut(s) 234, 242, 1478, 1923
BseNI ACTGG 4 cut(s) 333, 556, 875, 1368
BseRI GAGGAG 3 cut(s) 62, 1463, 2343
BseX3I CGGCCG 1 cut(s) 386
BseXI GCAGC 3 cut(s) 1276, 1523, 2380
BsgI GTGCAG 2 cut(s) 405, 834
Bsh1236I CGCG 1 cut(s) 2048
Bsh1285I CGRYCG 1 cut(s) 389
BshFI GGCC 6 cut(s) 332, 388, 700, 706, 1548, 2121
BshNI GGYRCC 1 cut(s) 887
BshTI ACCGGT 1 cut(s) 204
BsiEI CGRYCG 1 cut(s) 389
BsiHKAI GWGCWC 2 cut(s) 306, 2187
BsiSI CCGG 6 cut(s) 189, 205, 389, 862, 2039, 2397
BsiWI CGTACG 1 cut(s) 1264
BslFI GGGAC 4 cut(s) 110, 620, 844, 1997
BslI CCNNNNNNNGG 3 cut(s) 108, 602, 811
BsmAI GTCTC 1 cut(s) 1462
BsmFI GGGAC 4 cut(s) 110, 620, 844, 1997
BsnI GGCC 6 cut(s) 332, 388, 700, 706, 1548, 2121
Bsp119I TTCGAA 1 cut(s) 2297
Bsp1286I GDGCHC 2 cut(s) 306, 2187
Bsp1407I TGTACA 2 cut(s) 1236, 2284
Bsp143I GATC 8 cut(s) 631, 1304, 1492, 1900, 2094, 2140, 2149, 2429
Bsp19I CCATGG 1 cut(s) 1549
BspACI CCGC 3 cut(s) 1032, 1161, 2048
BspANI GGCC 6 cut(s) 332, 388, 700, 706, 1548, 2121
BspCNI CTCAG 4 cut(s) 235, 241, 1477, 1924
BspFNI CGCG 1 cut(s) 2048
BspHI TCATGA 1 cut(s) 2253
BspLI GGNNCC 4 cut(s) 113, 889, 1691, 1985
BspMI ACCTGC 1 cut(s) 2317
BspPI GGATC 2 cut(s) 2102, 2144
BspT104I TTCGAA 1 cut(s) 2297
BspT107I GGYRCC 1 cut(s) 887
BspTI CTTAAG 1 cut(s) 1141
BsrDI GCAATG 2 cut(s) 1921, 2357
BsrFI RCCGGY 2 cut(s) 204, 388
BsrGI TGTACA 2 cut(s) 1236, 2284
BsrI ACTGG 4 cut(s) 333, 556, 875, 1368
BssAI RCCGGY 2 cut(s) 204, 388
BssECI CCNNGG 7 cut(s) 93, 187, 595, 701, 944, 1549, 1978
BssMI GATC 8 cut(s) 631, 1304, 1492, 1900, 2094, 2140, 2149, 2429
BssSI CACGAG 2 cut(s) 1530, 1848
BssT1I CCWWGG 1 cut(s) 1549
Bst2BI CACGAG 2 cut(s) 1530, 1848
Bst2UI CCWGG 6 cut(s) 94, 458, 522, 597, 702, 945
Bst4CI ACNGT 5 cut(s) 9, 253, 882, 1098, 2455
Bst6I CTCTTC 2 cut(s) 71, 80
BstAFI CTTAAG 1 cut(s) 1141
BstAUI TGTACA 2 cut(s) 1236, 2284
BstBAI YACGTR 1 cut(s) 1264
BstBI TTCGAA 1 cut(s) 2297
BstC8I GCNNGC 4 cut(s) 409, 1338, 1389, 2373
BstDEI CTNAG 6 cut(s) 228, 243, 1464, 1932, 2206, 2364
BstDSI CCRYGG 2 cut(s) 1549, 1978
BstF5I GGATG 3 cut(s) 136, 466, 1004
BstFNI CGCG 1 cut(s) 2048
BstH2I RGCGCY 1 cut(s) 1825
BstHHI GCGC 2 cut(s) 1824, 2275
BstKTI GATC 8 cut(s) 634, 1307, 1495, 1903, 2097, 2143, 2152, 2432
BstMAI GTCTC 1 cut(s) 1462
BstMBI GATC 8 cut(s) 631, 1304, 1492, 1900, 2094, 2140, 2149, 2429
BstMCI CGRYCG 1 cut(s) 389
BstMWI GCNNNNNNNGC 5 cut(s) 1295, 1364, 1670, 2318, 2368
BstNI CCWGG 6 cut(s) 94, 458, 522, 597, 702, 945
BstNSI RCATGY 2 cut(s) 1756, 2035
BstSCI CCNGG 8 cut(s) 92, 187, 456, 520, 595, 700, 860, 943
BstSFI CTRYAG 1 cut(s) 504
BstSNI TACGTA 1 cut(s) 1264
BstUI CGCG 1 cut(s) 2048
BstV1I GCAGC 3 cut(s) 1276, 1523, 2380
BstX2I RGATCY 3 cut(s) 1900, 2149, 2429
BstYI RGATCY 3 cut(s) 1900, 2149, 2429
BstZI CGGCCG 1 cut(s) 386
BsuRI GGCC 6 cut(s) 332, 388, 700, 706, 1548, 2121
BtgI CCRYGG 2 cut(s) 1549, 1978
BtrI CACGTC 1 cut(s) 2174
BtsCI GGATG 3 cut(s) 136, 466, 1004
BtsIMutI CAGTG 4 cut(s) 5, 258, 887, 2400
BveI ACCTGC 1 cut(s) 2317
Cac8I GCNNGC 4 cut(s) 409, 1338, 1389, 2373
CciI TCATGA 1 cut(s) 2253
CfoI GCGC 2 cut(s) 1824, 2275
Cfr10I RCCGGY 2 cut(s) 204, 388
Cfr13I GGNCC 9 cut(s) 331, 369, 391, 698, 704, 1372, 1800, 1984, 2302
Csp6I GTAC 4 cut(s) 1237, 1265, 2044, 2285
CspAI ACCGGT 1 cut(s) 204
CviQI GTAC 4 cut(s) 1237, 1265, 2044, 2285
DdeI CTNAG 6 cut(s) 228, 243, 1464, 1932, 2206, 2364
DpnI GATC 8 cut(s) 633, 1306, 1494, 1902, 2096, 2142, 2151, 2431
DpnII GATC 8 cut(s) 631, 1304, 1492, 1900, 2094, 2140, 2149, 2429
DrdI GACNNNNNNGTC 1 cut(s) 2230
DseDI GACNNNNNNGTC 1 cut(s) 2230
EaeI YGGCCR 3 cut(s) 386, 1546, 2119
EagI CGGCCG 1 cut(s) 386
Eam1104I CTCTTC 2 cut(s) 71, 80
EarI CTCTTC 2 cut(s) 71, 80
Ecl136II GAGCTC 1 cut(s) 2185
EclXI CGGCCG 1 cut(s) 386
Eco105I TACGTA 1 cut(s) 1264
Eco130I CCWWGG 1 cut(s) 1549
Eco24I GRGCYC 1 cut(s) 2187
Eco47I GGWCC 6 cut(s) 369, 391, 1372, 1800, 1984, 2302
Eco47III AGCGCT 1 cut(s) 1823
Eco52I CGGCCG 1 cut(s) 386
Eco53kI GAGCTC 1 cut(s) 2185
Eco57I CTGAAG 3 cut(s) 146, 166, 1611
EcoICRI GAGCTC 1 cut(s) 2185
EcoO109I RGGNCCY 2 cut(s) 704, 1800
EcoRI GAATTC 1 cut(s) 1646
EcoRII CCWGG 6 cut(s) 92, 456, 520, 595, 700, 943
EcoT14I CCWWGG 1 cut(s) 1549
EcoT22I ATGCAT 2 cut(s) 484, 2264
EcoT38I GRGCYC 1 cut(s) 2187
ErhI CCWWGG 1 cut(s) 1549
FalI AAGNNNNNCTT 2 cut(s) 1650, 1682
FaqI GGGAC 4 cut(s) 110, 620, 844, 1997
FauI CCCGC 1 cut(s) 1025
FauNDI CATATG 2 cut(s) 1294, 2023
Fnu4HI GCNGC 3 cut(s) 1290, 1512, 2369
FokI GGATG 3 cut(s) 143, 473, 1011
FriOI GRGCYC 1 cut(s) 2187
Fsp4HI GCNGC 3 cut(s) 1290, 1512, 2369
FspBI CTAG 8 cut(s) 198, 557, 957, 1745, 1911, 1925, 1968, 2144
GlaI GCGC 2 cut(s) 1823, 2274
GluI GCNGC 3 cut(s) 1290, 1512, 2369
HaeII RGCGCY 1 cut(s) 1825
HaeIII GGCC 6 cut(s) 332, 388, 700, 706, 1548, 2121
HapII CCGG 6 cut(s) 189, 205, 389, 862, 2039, 2397
HhaI GCGC 2 cut(s) 1824, 2275
Hin6I GCGC 2 cut(s) 1822, 2273
HinP1I GCGC 2 cut(s) 1822, 2273
HindIII AAGCTT 1 cut(s) 1765
HinfI GANTC 9 cut(s) 89, 692, 785, 976, 1772, 1855, 1874, 2219, 2294
HpaII CCGG 6 cut(s) 189, 205, 389, 862, 2039, 2397
HphI GGTGA 4 cut(s) 550, 1859, 2028, 2354
Hpy166II GTNNAC 7 cut(s) 372, 941, 1237, 1239, 1250, 2285, 2383
Hpy188I TCNGA 9 cut(s) 80, 231, 244, 636, 981, 1467, 1954, 2018, 2077
Hpy188III TCNNGA 8 cut(s) 629, 725, 1076, 1496, 1629, 1878, 2144, 2254
Hpy8I GTNNAC 7 cut(s) 372, 941, 1237, 1239, 1250, 2285, 2383
Hpy99I CGWCG 1 cut(s) 778
HpyAV CCTTC 8 cut(s) 121, 829, 1178, 1791, 1813, 2163, 2189, 2293
HpyCH4III ACNGT 5 cut(s) 9, 253, 882, 1098, 2455
HpyCH4IV ACGT 4 cut(s) 776, 1263, 1267, 2173
HpyF10VI GCNNNNNNNGC 5 cut(s) 1295, 1364, 1670, 2318, 2368
HpyF3I CTNAG 6 cut(s) 228, 243, 1464, 1932, 2206, 2364
HpySE526I ACGT 4 cut(s) 776, 1263, 1267, 2173
HspAI GCGC 2 cut(s) 1822, 2273
Kzo9I GATC 8 cut(s) 631, 1304, 1492, 1900, 2094, 2140, 2149, 2429
LmnI GCTCC 2 cut(s) 1450, 2182
Lsp1109I GCAGC 3 cut(s) 1276, 1523, 2380
LweI GCATC 6 cut(s) 424, 1100, 1182, 1918, 2249, 2357
MaeI CTAG 8 cut(s) 198, 557, 957, 1745, 1911, 1925, 1968, 2144
MaeII ACGT 4 cut(s) 776, 1263, 1267, 2173
MaeIII GTNAC 6 cut(s) 381, 582, 715, 866, 2034, 2449
MalI GATC 8 cut(s) 633, 1306, 1494, 1902, 2096, 2142, 2151, 2431
MboI GATC 8 cut(s) 631, 1304, 1492, 1900, 2094, 2140, 2149, 2429
MflI RGATCY 3 cut(s) 1900, 2149, 2429
MhlI GDGCHC 2 cut(s) 306, 2187
MlsI TGGCCA 2 cut(s) 1548, 2121
MluNI TGGCCA 2 cut(s) 1548, 2121
MlyI GAGTC 3 cut(s) 701, 1766, 1868
MmeI TCCRAC 2 cut(s) 1996, 2085
Mox20I TGGCCA 2 cut(s) 1548, 2121
Mph1103I ATGCAT 2 cut(s) 484, 2264
MroXI GAANNNNTTC 3 cut(s) 1150, 2062, 2392
MscI TGGCCA 2 cut(s) 1548, 2121
MseI TTAA 7 cut(s) 104, 569, 918, 1068, 1142, 2114, 2165
MslI CAYNNNNRTG 1 cut(s) 377
Msp20I TGGCCA 2 cut(s) 1548, 2121
MspCI CTTAAG 1 cut(s) 1141
MspI CCGG 6 cut(s) 189, 205, 389, 862, 2039, 2397
MspR9I CCNGG 8 cut(s) 94, 189, 458, 522, 597, 702, 862, 945
MvaI CCWGG 6 cut(s) 94, 458, 522, 597, 702, 945
MvnI CGCG 1 cut(s) 2048
MwoI GCNNNNNNNGC 5 cut(s) 1295, 1364, 1670, 2318, 2368
NciI CCSGG 2 cut(s) 189, 862
NcoI CCATGG 1 cut(s) 1549
NdeI CATATG 2 cut(s) 1294, 2023
NdeII GATC 8 cut(s) 631, 1304, 1492, 1900, 2094, 2140, 2149, 2429
NlaIV GGNNCC 4 cut(s) 113, 889, 1691, 1985
NmuCI GTSAC 6 cut(s) 381, 582, 715, 866, 2034, 2449
NsiI ATGCAT 2 cut(s) 484, 2264
NspI RCATGY 2 cut(s) 1756, 2035
NspV TTCGAA 1 cut(s) 2297
OliI CACNNNNGTG 1 cut(s) 377
PagI TCATGA 1 cut(s) 2253
PciI ACATGT 1 cut(s) 2031
PdmI GAANNNNTTC 3 cut(s) 1150, 2062, 2392
PfeI GAWTC 6 cut(s) 89, 785, 976, 1855, 2219, 2294
Pfl23II CGTACG 1 cut(s) 1264
PfoI TCCNGGA 1 cut(s) 456
PinAI ACCGGT 1 cut(s) 204
PkrI GCNGC 3 cut(s) 1291, 1513, 2370
PleI GAGTC 3 cut(s) 700, 1766, 1868
PpsI GAGTC 3 cut(s) 700, 1766, 1868
Ppu21I YACGTR 1 cut(s) 1264
PpuMI RGGWCCY 1 cut(s) 1800
PscI ACATGT 1 cut(s) 2031
PshBI ATTAAT 1 cut(s) 918
PsiI TTATAA 2 cut(s) 345, 1572
Psp124BI GAGCTC 1 cut(s) 2187
Psp5II RGGWCCY 1 cut(s) 1800
Psp6I CCWGG 6 cut(s) 92, 456, 520, 595, 700, 943
PspGI CCWGG 6 cut(s) 92, 456, 520, 595, 700, 943
PspLI CGTACG 1 cut(s) 1264
PspN4I GGNNCC 4 cut(s) 113, 889, 1691, 1985
PspPI GGNCC 9 cut(s) 331, 369, 391, 698, 704, 1372, 1800, 1984, 2302
PspPPI RGGWCCY 1 cut(s) 1800
PsuI RGATCY 3 cut(s) 1900, 2149, 2429
RsaI GTAC 4 cut(s) 1238, 1266, 2045, 2286
RsaNI GTAC 4 cut(s) 1237, 1265, 2044, 2285
RseI CAYNNNNRTG 1 cut(s) 377
SacI GAGCTC 1 cut(s) 2187
SaqAI TTAA 7 cut(s) 104, 569, 918, 1068, 1142, 2114, 2165
SatI GCNGC 3 cut(s) 1290, 1512, 2369
Sau3AI GATC 8 cut(s) 631, 1304, 1492, 1900, 2094, 2140, 2149, 2429
Sau96I GGNCC 9 cut(s) 331, 369, 391, 698, 704, 1372, 1800, 1984, 2302
SchI GAGTC 3 cut(s) 701, 1766, 1868
ScrFI CCNGG 8 cut(s) 94, 189, 458, 522, 597, 702, 862, 945
SduI GDGCHC 2 cut(s) 306, 2187
SfaNI GCATC 6 cut(s) 424, 1100, 1182, 1918, 2249, 2357
SfcI CTRYAG 1 cut(s) 504
SfuI TTCGAA 1 cut(s) 2297
SinI GGWCC 6 cut(s) 369, 391, 1372, 1800, 1984, 2302
SmiMI CAYNNNNRTG 1 cut(s) 377
SmlI CTYRAG 3 cut(s) 1141, 1454, 1876
SmoI CTYRAG 3 cut(s) 1141, 1454, 1876
SnaBI TACGTA 1 cut(s) 1264
SsiI CCGC 3 cut(s) 1032, 1161, 2048
SspI AATATT 4 cut(s) 653, 916, 1350, 1974
SspMI CTAG 8 cut(s) 198, 557, 957, 1745, 1911, 1925, 1968, 2144
SstI GAGCTC 1 cut(s) 2187
StyD4I CCNGG 8 cut(s) 92, 187, 456, 520, 595, 700, 860, 943
StyI CCWWGG 1 cut(s) 1549
TaaI ACNGT 5 cut(s) 9, 253, 882, 1098, 2455
TaiI ACGT 4 cut(s) 779, 1266, 1270, 2176
TaqI TCGA 6 cut(s) 168, 1176, 1491, 2222, 2297, 2473
TaqII GACCGA 1 cut(s) 2319
TatI WGTACW 2 cut(s) 1236, 2284
TfiI GAWTC 6 cut(s) 89, 785, 976, 1855, 2219, 2294
Tru1I TTAA 7 cut(s) 104, 569, 918, 1068, 1142, 2114, 2165
Tru9I TTAA 7 cut(s) 104, 569, 918, 1068, 1142, 2114, 2165
TscAI CASTG 4 cut(s) 12, 258, 887, 2407
TseFI GTSAC 6 cut(s) 381, 582, 715, 866, 2034, 2449
TseI GCWGC 3 cut(s) 1289, 1511, 2368
Tsp45I GTSAC 6 cut(s) 381, 582, 715, 866, 2034, 2449
TspRI CASTG 4 cut(s) 12, 258, 887, 2407
Vha464I CTTAAG 1 cut(s) 1141
VpaK11BI GGWCC 6 cut(s) 369, 391, 1372, 1800, 1984, 2302
VspI ATTAAT 1 cut(s) 918
XapI RAATTY 7 cut(s) 193, 216, 442, 1070, 1646, 1947, 2392
XbaI TCTAGA 1 cut(s) 2143
XceI RCATGY 2 cut(s) 1756, 2035
XcmI CCANNNNNNNNNTGG 2 cut(s) 1556, 2129
XmnI GAANNNNTTC 3 cut(s) 1150, 2062, 2392
XspI CTAG 8 cut(s) 198, 557, 957, 1745, 1911, 1925, 1968, 2144
Zsp2I ATGCAT 2 cut(s) 484, 2264
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.