RchiOBHm_Chr3g0455681

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000112
Physical Location & Seq
Forward (+)
5339744 .. 5342928
3185 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ42260

Sequence Viewer

Length: 2472 bp
ATGCCACTGTTTCACACCTACAAAGTTGCTAATGCGCAGAAAATGTTATTCAAAGCTCTGCTTCTCTTCCTCCTCTTCCGATTTTGCGAATCCAGGGACACCATAACATGGAACCAAGAACAACTGAAGGATGGTGGTGGAGTTTTAGTGTCCAAACAGAGCAAGTTCGAATTGGGTTTCTTCAGCCCCGGAAATTCTAGCTACCGGTATGTCGGAATTTGGTATTCTCAGACTGAAGTATCTAATAAAACAGTGGTGTGGGTTGCAAACAGGAACAATCCCATCAATGATACCTCTGGTGTGCTCACAATAAACAGGTATGGAGAACTAGTCCTTTATGCTTATAACATGGAGAGCACTCCTATTTGGTCTACTTATGTGTCGCGGTCGGTTCAAAATGTTAACACAACCACTTTATCTGCACAGCTTTTAGATACAGGAAATCTAGTTGTGTTCCAGGATAATAATAATGAAATCTCTATATGGCAAAGTTTTGATCATCCTACAGATACTTTAATTCGAGGTATGAAAGTTGGGGTGAACTGGAAAAGTGGGCAAGAATGGGTTTTAACGTCTTGGAAGTCACAAGATGACCCTGGAACTGGGGACTATACCCTTAGGCTCTATCCAAATCAGATTGCATCCCCCCAATTTTTTATGTACAAGGGGTCGAGTAAGTATTGGCGAAGTGATACAGGGCCAATTGATTTTGTCACTAATCAAGAGGAGACTTATTATCTCATGAATGGCACCAATACAATTACAAGAGCAACAGTGACTGATTCTGCAGTAGAACGCCTTACATGGGATGATGGTGGCCTTCAATGGGAGGAAGACTTCTCTGCACCGAAGTCCCGGTGTGACTGGTATGGACAGTGCGGTGCCAACAGCAAATGTAGCCCTGACAATGTTAATGTGTTTTGGTGTGATTGTTTACCAGGGTATGTACCTAAATCTATTAGTGATTGGAATCATAAAAATGGGACAGGTGGATGTGTGAGTAATCGACTTGGTTTGTTGAAGTGTGGAGATGGAGACGGGTTTGTGAAGGTGGCAAGAGTTAAATATCCTGACACATCGATAGGAGCATTGTTAAAATCAGGTATGAGTGCCAAAGAGTGTGAGCAGGAGTGCCTAAGAAATTGTTCTTGCACCGCATATTTGAGCACTGAATATGAAGGGCTTGTTGATTGCTTGACATGGTATGATGACTTGATGGACATTTTAGTGTACACAGAGGTTGGACAAGATCTCTACGTTCGTGTGAATGCAACCGTGTTAGCTGCAAATGCCAGAAGATCAGAAGGTTTTTTGGAAAGGAGGGGTATGCTGGCTATTCCAATACTGTCTGCTGTGCTAGCATTGGTGCTAATCATTATGCTTGCATGTTGGTGGGGTAAGAAGAACAGGAACACAAAAGGCATAGACTTTGTGGAGGCAGACGAGCTTGTGGAAAACCAGACAAACCCTGAATTGCAATTTTTCGATCTTGACACAATAATAGCAGCCACAGACCACTTCTCTCGTGTCAATGAACTTGGCCATGGTGGTTTTGGCTCTGTTTATAAGGGTCAGCTACCAAATGAAGAGAAAGTTGCTGTGAAAAGATTGTCCCAAACTTCAGGACAAGGGATCAAAGAATTCAAAAATGAAGTTGCAGTTATAGCAAGACTTCAACACAGGAATCTTGTGAAACTTTTAGGCTGTTGTATAAAGGGAGAAGAAAGGATATTAGTGCTAGAATACATGCCTAACAAAAGCTTGAACTCCTTTCTTTTCGATAGCACAAGACGGTCCTTTTTGGATTGGGAAAAGCGCTTTGAAATTATCAACGGGATTGCTCGTGGGATTTTGTATCTTCACCAAGACTCAAGATTGAGGATCATCCATAGAGATCTGAAAACTAGCAATATTCTTCTAGATACGAAGATGAACCCAAAAATTTCTGATTTTGGCATGGCTAGAATATTCCACGGAGATCAACTGCAAGATAAGACGAGCCGAATTGTTGGAACATATGGTTACATGTCACCAGAGTACGCAATATTTGGAAGATTTTCTACGAAATCTGATATATTTAGTTTTGGGATCATAATGTTGGAGATTGTTAGTGGCCGGAAAAACAATGGTTATGATCTGGAGGATCCTTCCATGAACTTGATAGGACGTGTTTGGGAGCTCTGGGGAGAAGGCAGGGCCTTAGATATTGTTGATTCGACACTGAAGTCATATAAGCCTAATGAAGTCATGAGATGTATACAAGTTGCGCTTTTGTGTGTACAAGAAGATTCAAAGGACCGACCTGCCATTTCAGCCATTGTTTTCATGTTGAGTGGTGAATTATCTCCTCCATTGCCTAAGCATCCCGCATTTGTTTACAGAAGAGATTTCAGCGCTGATGTTGATCCATTACTTCCAAACGGATCTGATTCTGTAAATGACTTGACAATAACTACAATGGAAGTTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

823

Amino Acids

93.02

Weight (kDa)

5.7

Isoelectric Point (pI)

35.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 84 - 194 1.7e-34 D-mannose binding lectin
S_locus_glycop PF00954 229 - 319 9.8e-13 S-locus glycoprotein domain
PAN_2 PF08276 342 - 408 1.1e-17 PAN-like domain
Pkinase PF00069 508 - 771 3.1e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 510 - 775 1.3e-49 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 345, 1566
AasI GACNNNNNNGTC 1 cut(s) 2224
Acc16I TGCGCA 1 cut(s) 36
Acc36I ACCTGC 1 cut(s) 2311
AccB1I GGYRCC 2 cut(s) 749, 881
AccB7I CCANNNNNTGG 1 cut(s) 108
AccI GTMKAC 2 cut(s) 371, 2257
AccII CGCG 1 cut(s) 385
AciI CCGC 4 cut(s) 385, 879, 1155, 2367
AclWI GGATC 7 cut(s) 1640, 1889, 2096, 2138, 2151, 2399, 2431
AcoI YGGCCR 2 cut(s) 1540, 2113
AcsI RAATTY 4 cut(s) 193, 216, 1640, 1941
AcuI CTGAAG 5 cut(s) 146, 166, 255, 1605, 2243
AfaI GTAC 5 cut(s) 662, 948, 1232, 2039, 2280
AfeI AGCGCT 2 cut(s) 1817, 2395
AfiI CCNNNNNNNGG 4 cut(s) 108, 602, 805, 826
AflIII ACRYGT 2 cut(s) 2025, 2167
AgeI ACCGGT 1 cut(s) 204
AgsI TTSAA 9 cut(s) 52, 395, 824, 1021, 1645, 1676, 1765, 1823, 2292
AhlI ACTAGT 1 cut(s) 328
AjiI CACGTC 1 cut(s) 2168
AjnI CCWGG 4 cut(s) 92, 456, 595, 937
AluBI AGCT 8 cut(s) 56, 201, 427, 1283, 1447, 1576, 1761, 2179
AluI AGCT 8 cut(s) 56, 201, 427, 1283, 1447, 1576, 1761, 2179
Alw21I GWGCWC 4 cut(s) 306, 359, 1169, 2181
Alw26I GTCTC 2 cut(s) 722, 1029
AlwI GGATC 7 cut(s) 1640, 1889, 2096, 2138, 2151, 2399, 2431
AlwNI CAGNNNCTG 1 cut(s) 779
Aor51HI AGCGCT 2 cut(s) 1817, 2395
AoxI GGCC 5 cut(s) 698, 817, 1540, 2113, 2196
ApeKI GCWGC 2 cut(s) 1283, 1505
ApoI RAATTY 4 cut(s) 193, 216, 1640, 1941
AsiGI ACCGGT 1 cut(s) 204
Asp700I GAANNNNTTC 2 cut(s) 1144, 2056
AspLEI GCGC 4 cut(s) 37, 1818, 2269, 2396
AspS9I GGNCC 4 cut(s) 698, 1794, 2196, 2296
AsuC2I CCSGG 2 cut(s) 189, 856
AsuHPI GGTGA 4 cut(s) 550, 1853, 2022, 2348
AsuII TTCGAA 1 cut(s) 168
AsuNHI GCTAGC 1 cut(s) 1357
AvaII GGWCC 2 cut(s) 1794, 2296
AxyI CCTNAGG 1 cut(s) 617
BaeI ACNNNNGTAYC 2 cut(s) 222, 255
BalI TGGCCA 1 cut(s) 1542
BamHI GGATCC 1 cut(s) 2143
BanI GGYRCC 2 cut(s) 749, 881
BanII GRGCYC 1 cut(s) 2181
BarI GAAGNNNNNNTAC 2 cut(s) 2044, 2076
BauI CACGAG 2 cut(s) 1524, 1842
BbsI GAAGAC 1 cut(s) 840
Bbv12I GWGCWC 4 cut(s) 306, 359, 1169, 2181
BbvI GCAGC 2 cut(s) 1270, 1517
BccI CCATC 5 cut(s) 125, 290, 806, 1025, 1210
BciT130I CCWGG 4 cut(s) 94, 458, 597, 939
BclI TGATCA 1 cut(s) 496
BcnI CCSGG 2 cut(s) 189, 856
BcoDI GTCTC 2 cut(s) 722, 1029
BcuI ACTAGT 1 cut(s) 328
BfaI CTAG 8 cut(s) 198, 329, 446, 1358, 1739, 1905, 1919, 1962
BfmI CTRYAG 2 cut(s) 504, 786
BfoI RGCGCY 2 cut(s) 1819, 2397
BfuAI ACCTGC 1 cut(s) 2311
BglII AGATCT 2 cut(s) 1249, 1894
BisI GCNGC 2 cut(s) 1284, 1506
BlsI GCNGC 2 cut(s) 1285, 1507
Bme1390I CCNGG 6 cut(s) 94, 189, 458, 597, 856, 939
Bme18I GGWCC 2 cut(s) 1794, 2296
BmgBI CACGTC 1 cut(s) 2168
BmgT120I GGNCC 4 cut(s) 698, 1794, 2196, 2296
BmiI GGNNCC 4 cut(s) 113, 751, 883, 2145
BmrFI CCNGG 6 cut(s) 94, 189, 458, 597, 856, 939
BmrI ACTGGG 1 cut(s) 612
BmsI GCATC 2 cut(s) 650, 2371
BmtI GCTAGC 1 cut(s) 1361
BmuI ACTGGG 1 cut(s) 612
BpiI GAAGAC 1 cut(s) 840
BpmI CTGGAG 1 cut(s) 2159
Bpu10I CCTNAGC 1 cut(s) 2358
Bpu14I TTCGAA 1 cut(s) 168
BpuEI CTTGAG 1 cut(s) 1855
BpuMI CCSGG 2 cut(s) 189, 856
Bsa29I ATCGAT 1 cut(s) 1079
BsaBI GATNNNNATC 2 cut(s) 969, 2403
BsaJI CCNNGG 6 cut(s) 93, 187, 595, 938, 1543, 1972
BsaWI WCCGGW 1 cut(s) 204
BsaXI ACNNNNNCTCC 4 cut(s) 132, 162, 315, 345
Bsc4I CCNNNNNNNGG 4 cut(s) 108, 602, 805, 826
Bse118I RCCGGY 1 cut(s) 204
Bse1I ACTGG 3 cut(s) 548, 607, 869
Bse21I CCTNAGG 1 cut(s) 617
Bse3DI GCAATG 1 cut(s) 2351
Bse8I GATNNNNATC 2 cut(s) 969, 2403
BseBI CCWGG 4 cut(s) 94, 458, 597, 939
BseCI ATCGAT 1 cut(s) 1079
BseDI CCNNGG 6 cut(s) 93, 187, 595, 938, 1543, 1972
BseGI GGATG 7 cut(s) 136, 499, 641, 814, 998, 1884, 2362
BseJI GATNNNNATC 2 cut(s) 969, 2403
BseLI CCNNNNNNNGG 4 cut(s) 108, 602, 805, 826
BseMI GCAATG 1 cut(s) 2351
BseMII CTCAG 1 cut(s) 242
BseNI ACTGG 3 cut(s) 548, 607, 869
BseRI GAGGAG 3 cut(s) 62, 740, 2337
BseXI GCAGC 2 cut(s) 1270, 1517
BsgI GTGCAG 2 cut(s) 405, 828
Bsh1236I CGCG 1 cut(s) 385
Bsh1285I CGRYCG 1 cut(s) 389
BshFI GGCC 5 cut(s) 700, 819, 1542, 2115, 2198
BshNI GGYRCC 2 cut(s) 749, 881
BshTI ACCGGT 1 cut(s) 204
BshVI ATCGAT 1 cut(s) 1079
BsiEI CGRYCG 1 cut(s) 389
BsiHKAI GWGCWC 4 cut(s) 306, 359, 1169, 2181
BsiSI CCGG 4 cut(s) 189, 205, 856, 2116
BslFI GGGAC 5 cut(s) 110, 620, 838, 997, 1597
BslI CCNNNNNNNGG 4 cut(s) 108, 602, 805, 826
BsmAI GTCTC 2 cut(s) 722, 1029
BsmBI CGTCTC 1 cut(s) 1029
BsmFI GGGAC 5 cut(s) 110, 620, 838, 997, 1597
BsmI GAATGC 1 cut(s) 1273
BsnI GGCC 5 cut(s) 700, 819, 1542, 2115, 2198
Bsp119I TTCGAA 1 cut(s) 168
Bsp1286I GDGCHC 4 cut(s) 306, 359, 1169, 2181
Bsp1407I TGTACA 3 cut(s) 660, 1230, 2278
Bsp19I CCATGG 1 cut(s) 1543
BspACI CCGC 4 cut(s) 385, 879, 1155, 2367
BspANI GGCC 5 cut(s) 700, 819, 1542, 2115, 2198
BspCNI CTCAG 1 cut(s) 241
BspDI ATCGAT 1 cut(s) 1079
BspFNI CGCG 1 cut(s) 385
BspHI TCATGA 2 cut(s) 741, 2247
BspLI GGNNCC 4 cut(s) 113, 751, 883, 2145
BspMAI CTGCAG 1 cut(s) 790
BspMI ACCTGC 1 cut(s) 2311
BspOI GCTAGC 1 cut(s) 1361
BspPI GGATC 7 cut(s) 1640, 1889, 2096, 2138, 2151, 2399, 2431
BspT104I TTCGAA 1 cut(s) 168
BspT107I GGYRCC 2 cut(s) 749, 881
BsrDI GCAATG 1 cut(s) 2351
BsrFI RCCGGY 1 cut(s) 204
BsrGI TGTACA 3 cut(s) 660, 1230, 2278
BsrI ACTGG 3 cut(s) 548, 607, 869
BssAI RCCGGY 1 cut(s) 204
BssECI CCNNGG 6 cut(s) 93, 187, 595, 938, 1543, 1972
BssNAI GTATAC 1 cut(s) 2258
BssSI CACGAG 2 cut(s) 1524, 1842
BssT1I CCWWGG 1 cut(s) 1543
Bst1107I GTATAC 1 cut(s) 2258
Bst2BI CACGAG 2 cut(s) 1524, 1842
Bst2UI CCWGG 4 cut(s) 94, 458, 597, 939
Bst4CI ACNGT 7 cut(s) 9, 253, 775, 876, 1276, 1347, 1794
Bst6I CTCTTC 4 cut(s) 71, 80, 1581, 2377
BstAUI TGTACA 3 cut(s) 660, 1230, 2278
BstBI TTCGAA 1 cut(s) 168
BstC8I GCNNGC 3 cut(s) 1332, 1359, 1383
BstDEI CTNAG 5 cut(s) 228, 617, 1136, 2200, 2358
BstDSI CCRYGG 2 cut(s) 1543, 1972
BstF5I GGATG 7 cut(s) 136, 499, 641, 814, 998, 1884, 2362
BstFNI CGCG 1 cut(s) 385
BstH2I RGCGCY 2 cut(s) 1819, 2397
BstHHI GCGC 4 cut(s) 37, 1818, 2269, 2396
BstMAI GTCTC 2 cut(s) 722, 1029
BstMCI CGRYCG 1 cut(s) 389
BstMWI GCNNNNNNNGC 5 cut(s) 897, 1289, 1358, 1664, 2312
BstNI CCWGG 4 cut(s) 94, 458, 597, 939
BstNSI RCATGY 3 cut(s) 1389, 1750, 2029
BstSCI CCNGG 6 cut(s) 92, 187, 456, 595, 854, 937
BstSFI CTRYAG 2 cut(s) 504, 786
BstUI CGCG 1 cut(s) 385
BstV1I GCAGC 2 cut(s) 1270, 1517
BstV2I GAAGAC 1 cut(s) 840
BstX2I RGATCY 4 cut(s) 1249, 1894, 2143, 2423
BstYI RGATCY 4 cut(s) 1249, 1894, 2143, 2423
BstZ17I GTATAC 1 cut(s) 2258
Bsu15I ATCGAT 1 cut(s) 1079
Bsu36I CCTNAGG 1 cut(s) 617
BsuRI GGCC 5 cut(s) 700, 819, 1542, 2115, 2198
BsuTUI ATCGAT 1 cut(s) 1079
BtgI CCRYGG 2 cut(s) 1543, 1972
BtrI CACGTC 1 cut(s) 2168
BtsCI GGATG 7 cut(s) 136, 499, 641, 814, 998, 1884, 2362
BtsIMutI CAGTG 6 cut(s) 5, 258, 780, 881, 1167, 2219
BveI ACCTGC 1 cut(s) 2311
Cac8I GCNNGC 3 cut(s) 1332, 1359, 1383
CaiI CAGNNNCTG 1 cut(s) 779
CciI TCATGA 2 cut(s) 741, 2247
CfoI GCGC 4 cut(s) 37, 1818, 2269, 2396
Cfr10I RCCGGY 1 cut(s) 204
Cfr13I GGNCC 4 cut(s) 698, 1794, 2196, 2296
ClaI ATCGAT 1 cut(s) 1079
Csp6I GTAC 5 cut(s) 661, 947, 1231, 2038, 2279
CspAI ACCGGT 1 cut(s) 204
CviQI GTAC 5 cut(s) 661, 947, 1231, 2038, 2279
DdeI CTNAG 5 cut(s) 228, 617, 1136, 2200, 2358
DrdI GACNNNNNNGTC 1 cut(s) 2224
DseDI GACNNNNNNGTC 1 cut(s) 2224
EaeI YGGCCR 2 cut(s) 1540, 2113
Eam1104I CTCTTC 4 cut(s) 71, 80, 1581, 2377
EarI CTCTTC 4 cut(s) 71, 80, 1581, 2377
Ecl136II GAGCTC 1 cut(s) 2179
Eco130I CCWWGG 1 cut(s) 1543
Eco24I GRGCYC 1 cut(s) 2181
Eco47I GGWCC 2 cut(s) 1794, 2296
Eco47III AGCGCT 2 cut(s) 1817, 2395
Eco53kI GAGCTC 1 cut(s) 2179
Eco57I CTGAAG 5 cut(s) 146, 166, 255, 1605, 2243
Eco81I CCTNAGG 1 cut(s) 617
EcoICRI GAGCTC 1 cut(s) 2179
EcoO109I RGGNCCY 1 cut(s) 2196
EcoRI GAATTC 1 cut(s) 1640
EcoRII CCWGG 4 cut(s) 92, 456, 595, 937
EcoT14I CCWWGG 1 cut(s) 1543
EcoT38I GRGCYC 1 cut(s) 2181
ErhI CCWWGG 1 cut(s) 1543
Esp3I CGTCTC 1 cut(s) 1029
FalI AAGNNNNNCTT 4 cut(s) 45, 77, 2253, 2285
FaqI GGGAC 5 cut(s) 110, 620, 838, 997, 1597
FauI CCCGC 1 cut(s) 2374
FauNDI CATATG 1 cut(s) 2017
FbaI TGATCA 1 cut(s) 496
FblI GTMKAC 2 cut(s) 371, 2257
Fnu4HI GCNGC 2 cut(s) 1284, 1506
FokI GGATG 7 cut(s) 143, 486, 628, 821, 1005, 1871, 2349
FriOI GRGCYC 1 cut(s) 2181
Fsp4HI GCNGC 2 cut(s) 1284, 1506
FspBI CTAG 8 cut(s) 198, 329, 446, 1358, 1739, 1905, 1919, 1962
FspI TGCGCA 1 cut(s) 36
GlaI GCGC 4 cut(s) 36, 1817, 2268, 2395
GluI GCNGC 2 cut(s) 1284, 1506
GsuI CTGGAG 1 cut(s) 2159
HaeII RGCGCY 2 cut(s) 1819, 2397
HaeIII GGCC 5 cut(s) 700, 819, 1542, 2115, 2198
HapII CCGG 4 cut(s) 189, 205, 856, 2116
HhaI GCGC 4 cut(s) 37, 1818, 2269, 2396
Hin6I GCGC 4 cut(s) 35, 1816, 2267, 2394
HinP1I GCGC 4 cut(s) 35, 1816, 2267, 2394
HincII GTYRAC 1 cut(s) 403
HindII GTYRAC 1 cut(s) 403
HindIII AAGCTT 1 cut(s) 1759
HinfI GANTC 8 cut(s) 89, 782, 970, 1684, 1868, 2213, 2288, 2429
HpaI GTTAAC 1 cut(s) 403
HpaII CCGG 4 cut(s) 189, 205, 856, 2116
HphI GGTGA 4 cut(s) 550, 1853, 2022, 2348
Hpy166II GTNNAC 9 cut(s) 372, 403, 541, 935, 1231, 1233, 2258, 2279, 2377
Hpy188I TCNGA 9 cut(s) 80, 215, 231, 636, 1303, 1899, 1948, 2071, 2428
Hpy188III TCNNGA 9 cut(s) 722, 742, 1070, 1490, 1623, 1872, 1919, 2138, 2248
Hpy8I GTNNAC 9 cut(s) 372, 403, 541, 935, 1231, 1233, 2258, 2279, 2377
HpyAV CCTTC 7 cut(s) 121, 830, 1042, 1172, 1298, 2157, 2183
HpyCH4III ACNGT 7 cut(s) 9, 253, 775, 876, 1276, 1347, 1794
HpyCH4IV ACGT 3 cut(s) 572, 1257, 2167
HpyF10VI GCNNNNNNNGC 5 cut(s) 897, 1289, 1358, 1664, 2312
HpyF3I CTNAG 5 cut(s) 228, 617, 1136, 2200, 2358
HpySE526I ACGT 3 cut(s) 572, 1257, 2167
HspAI GCGC 4 cut(s) 35, 1816, 2267, 2394
Ksp22I TGATCA 1 cut(s) 496
KspAI GTTAAC 1 cut(s) 403
LmnI GCTCC 2 cut(s) 1085, 2176
Lsp1109I GCAGC 2 cut(s) 1270, 1517
LweI GCATC 2 cut(s) 650, 2371
MaeI CTAG 8 cut(s) 198, 329, 446, 1358, 1739, 1905, 1919, 1962
MaeII ACGT 3 cut(s) 572, 1257, 2167
MaeIII GTNAC 6 cut(s) 582, 712, 775, 860, 2021, 2028
MfeI CAATTG 1 cut(s) 702
MflI RGATCY 4 cut(s) 1249, 1894, 2143, 2423
MhlI GDGCHC 4 cut(s) 306, 359, 1169, 2181
MlsI TGGCCA 1 cut(s) 1542
MluNI TGGCCA 1 cut(s) 1542
MlyI GAGTC 1 cut(s) 1862
MmeI TCCRAC 4 cut(s) 193, 1222, 1990, 2079
Mox20I TGGCCA 1 cut(s) 1542
MroXI GAANNNNTTC 2 cut(s) 1144, 2056
MscI TGGCCA 1 cut(s) 1542
MseI TTAA 6 cut(s) 402, 515, 569, 912, 1062, 1094
MslI CAYNNNNRTG 3 cut(s) 978, 1226, 1390
Msp20I TGGCCA 1 cut(s) 1542
MspI CCGG 4 cut(s) 189, 205, 856, 2116
MspR9I CCNGG 6 cut(s) 94, 189, 458, 597, 856, 939
MunI CAATTG 1 cut(s) 702
Mva1269I GAATGC 1 cut(s) 1273
MvaI CCWGG 4 cut(s) 94, 458, 597, 939
MvnI CGCG 1 cut(s) 385
MwoI GCNNNNNNNGC 5 cut(s) 897, 1289, 1358, 1664, 2312
NciI CCSGG 2 cut(s) 189, 856
NcoI CCATGG 1 cut(s) 1543
NdeI CATATG 1 cut(s) 2017
NheI GCTAGC 1 cut(s) 1357
NlaIV GGNNCC 4 cut(s) 113, 751, 883, 2145
NmuCI GTSAC 5 cut(s) 582, 712, 775, 860, 2028
NsbI TGCGCA 1 cut(s) 36
NspI RCATGY 3 cut(s) 1389, 1750, 2029
NspV TTCGAA 1 cut(s) 168
PagI TCATGA 2 cut(s) 741, 2247
PciI ACATGT 1 cut(s) 2025
PctI GAATGC 1 cut(s) 1273
PdmI GAANNNNTTC 2 cut(s) 1144, 2056
PfeI GAWTC 7 cut(s) 89, 782, 970, 1684, 2213, 2288, 2429
PflMI CCANNNNNTGG 1 cut(s) 108
PfoI TCCNGGA 1 cut(s) 456
PinAI ACCGGT 1 cut(s) 204
PkrI GCNGC 2 cut(s) 1285, 1507
PleI GAGTC 1 cut(s) 1862
PpsI GAGTC 1 cut(s) 1862
PscI ACATGT 1 cut(s) 2025
PsiI TTATAA 2 cut(s) 345, 1566
Psp124BI GAGCTC 1 cut(s) 2181
Psp6I CCWGG 4 cut(s) 92, 456, 595, 937
PspGI CCWGG 4 cut(s) 92, 456, 595, 937
PspN4I GGNNCC 4 cut(s) 113, 751, 883, 2145
PspPI GGNCC 4 cut(s) 698, 1794, 2196, 2296
PstI CTGCAG 1 cut(s) 790
PstNI CAGNNNCTG 1 cut(s) 779
PsuI RGATCY 4 cut(s) 1249, 1894, 2143, 2423
RsaI GTAC 5 cut(s) 662, 948, 1232, 2039, 2280
RsaNI GTAC 5 cut(s) 661, 947, 1231, 2038, 2279
RseI CAYNNNNRTG 3 cut(s) 978, 1226, 1390
SacI GAGCTC 1 cut(s) 2181
SaqAI TTAA 6 cut(s) 402, 515, 569, 912, 1062, 1094
SatI GCNGC 2 cut(s) 1284, 1506
Sau96I GGNCC 4 cut(s) 698, 1794, 2196, 2296
SchI GAGTC 1 cut(s) 1862
ScrFI CCNGG 6 cut(s) 94, 189, 458, 597, 856, 939
SduI GDGCHC 4 cut(s) 306, 359, 1169, 2181
SfaNI GCATC 2 cut(s) 650, 2371
SfcI CTRYAG 2 cut(s) 504, 786
SfuI TTCGAA 1 cut(s) 168
SinI GGWCC 2 cut(s) 1794, 2296
SmiMI CAYNNNNRTG 3 cut(s) 978, 1226, 1390
SmlI CTYRAG 1 cut(s) 1870
SmoI CTYRAG 1 cut(s) 1870
SpeI ACTAGT 1 cut(s) 328
SsiI CCGC 4 cut(s) 385, 879, 1155, 2367
SspI AATATT 3 cut(s) 1912, 1968, 2046
SspMI CTAG 8 cut(s) 198, 329, 446, 1358, 1739, 1905, 1919, 1962
SstI GAGCTC 1 cut(s) 2181
StyD4I CCNGG 6 cut(s) 92, 187, 456, 595, 854, 937
StyI CCWWGG 1 cut(s) 1543
TaaI ACNGT 7 cut(s) 9, 253, 775, 876, 1276, 1347, 1794
TaiI ACGT 3 cut(s) 575, 1260, 2170
TaqI TCGA 9 cut(s) 168, 520, 671, 1006, 1079, 1485, 1779, 2216, 2467
TaqII GACCGA 1 cut(s) 2313
TatI WGTACW 3 cut(s) 660, 1230, 2278
TfiI GAWTC 7 cut(s) 89, 782, 970, 1684, 2213, 2288, 2429
Tru1I TTAA 6 cut(s) 402, 515, 569, 912, 1062, 1094
Tru9I TTAA 6 cut(s) 402, 515, 569, 912, 1062, 1094
TscAI CASTG 6 cut(s) 12, 258, 780, 881, 1174, 2226
TseFI GTSAC 5 cut(s) 582, 712, 775, 860, 2028
TseI GCWGC 2 cut(s) 1283, 1505
Tsp45I GTSAC 5 cut(s) 582, 712, 775, 860, 2028
TspGWI ACGGA 2 cut(s) 1989, 2436
TspRI CASTG 6 cut(s) 12, 258, 780, 881, 1174, 2226
Van91I CCANNNNNTGG 1 cut(s) 108
VpaK11BI GGWCC 2 cut(s) 1794, 2296
XapI RAATTY 4 cut(s) 193, 216, 1640, 1941
XbaI TCTAGA 1 cut(s) 1918
XceI RCATGY 3 cut(s) 1389, 1750, 2029
XcmI CCANNNNNNNNNTGG 1 cut(s) 1550
XmiI GTMKAC 2 cut(s) 371, 2257
XmnI GAANNNNTTC 2 cut(s) 1144, 2056
XspI CTAG 8 cut(s) 198, 329, 446, 1358, 1739, 1905, 1919, 1962
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.