RLG00000031291

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000112
Physical Location & Seq
Forward (+)
3795171 .. 3798880
3710 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031291

Sequence Viewer

Length: 1938 bp
ATGTTATTCAAAGCTCTGCTTCTCTTCCTCCTCTTCCGACTTTGCGAATCCAGGGACACCATAACATGGAACCAAGAACAACTGAAGGATGATGGTGGAGTTTTAGTGTCCAAACAGAGCAAGTTCGAATTGGGTTTCTTCAGCCCCGGAAATTCTAGCAACCGGTATGTTGGAATTTGGTATTCTCAGACTAGAGTATCTAATAAAACAGTGGTGTGGGTTGCAAACAGGAACAATCCCATCAATAATACCTCTGGTGTGCTCAAAATAAACAGGTATGGAGAACTAGTCCTTTATGCTTATAACATGGAGAGCACTCCTGTTTGGTCTACTAATGTGTCGCGGTCGGTTCAAAATATTAACACAAGCACTTTATCTGCACAGCTTTTAGATACAGGAAATCTAGTTGTGTTCCAGGATGATAATAATGAAAACCTTTTATGGCAAAGTTTTGATCATCCCACAGATACTTTAATTCCAGGTATGAAAGTTGGGGTGAATTGGAAAACTGGGCAAGAATGGGTTTTAACATCTTGGAAGTCACAAGATGACCCTGCAATTGGGGACTATACCTTAAGGCTCTATCCAAATCAGATTGCATCCCCCGAATTTTTTCTGTACAAGGGTTTGAGTAAGTATTGGCGAAGTCCAGGGCCATCACCTAGTGTGGTCACTAATCAAGAGGAAACTTATTATCTCATGGATGACACCAATGCAATTACAAGAGCAACCGTGACTGATTCTGCGCACGAGCGCTTTACATGGGATGATGGTGGCCTTCAATGGAAGGAAGACTACTCTGAACCGAAGTCCCTGTGTGACTGGTATGGACAGTGTGGTGCCAACAGCAAATGTAGCCCTGACAACATTAATGCTATGCTGTTTTGGTGTGATTGTTTACCAGGGTATGTGCCTAATTCTGTAAGTGATTGGAATCAGAAAAATGGGTCAGGTGGATGTGTGAGTAATCGACTTGGTTTGTTGAAGTGTGGTGATGGAGACGGGTTTGTGAAGGTGGCAAGAGTTAAATATCCGGACACATCGATAGCAGCATTGTTAAAATCAGGAATGAGTGACAAAGAGTGTGAGCAGGAGTGCCTAAGAAATTGTTCTTGCACTGCATATTTGAGCACTGAAAATGAAGGGACTGTTGATTGCTTGACATGGTATGATGACTTGATGGACATTTTAGTGTACACAGAGCTTGGACAAGATCTCTACGTTCGTGTGAATGCAACTGTGCTAGATGACACAAGACGGTCCTTCTTGGATTGGGAAAAGCGCTTTGAAATTATCAACGGGATTGCTCGTGGGATTTTGTATCTTCACCAAGACTCAAGATTGAGGATCATCCATAGAGATCTGAAAACTAGTAATATTCTTCTAGATGCTGAGATGAACCCAAGAATTTCTGATTTTGGCATGGCTAGAATATTCCATGGAGACCAATTGCAAGATAAGACAAGGCGAATTGTTGGAACATATGGCTACATGTCACCCGAGTATGCAGTATTTGGGAGATTTTCAACGAAATCTGATGTATTTAGTTTTGGGATCATAATGTTGGAGATTGTAAGCGGCCGGAAAAACAATGGTTCTGATGTAGAGGATCCTTCCATGAACTTGATAGGACGTGTTTGGGAGCTATGGGGAGAAGGCAGGGCCTTAGATATTGTTGATTCGACACTGAAGTCATATCAGCCTAATGAAGTCATGAGATGCATACAAGTTGCGCTCTTGTGTGTACAAGAAGATTCAAAGGACCGACCTGCCATGTCAGCCATTGTTTTCATGTTGAGTGGTGAAGCATCTCCTCCATTGCCTAAGCATCCGGCATTTGTTTACAGAAGAGATTCCGACGCTGATGTTGATCCATTACATGCAAACGGAACTGATTCTATAAATGACTTGACAATAACTACAATGAAAGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

646

Amino Acids

72.91

Weight (kDa)

5.01

Isoelectric Point (pI)

38.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 70 - 180 1.3e-36 D-mannose binding lectin
S_locus_glycop PF00954 229 - 306 1.1e-11 S-locus glycoprotein domain
PAN_2 PF08276 330 - 396 2.5e-18 PAN-like domain
Pkinase PF00069 365 - 595 1.8e-25 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 413 - 597 2.8e-26 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 303
AasI GACNNNNNNGTC 1 cut(s) 1690
Acc16I TGCGCA 1 cut(s) 747
Acc36I ACCTGC 1 cut(s) 1777
AccB1I GGYRCC 1 cut(s) 839
AccB7I CCANNNNNTGG 1 cut(s) 66
AccI GTMKAC 1 cut(s) 329
AccII CGCG 1 cut(s) 343
AccIII TCCGGA 1 cut(s) 1033
AciI CCGC 2 cut(s) 343, 1578
AclWI GGATC 5 cut(s) 1355, 1562, 1604, 1617, 1865
AcoI YGGCCR 1 cut(s) 1579
AcsI RAATTY 4 cut(s) 151, 174, 608, 1407
AcuI CTGAAG 3 cut(s) 104, 124, 1709
AdeI CACNNNGTG 1 cut(s) 665
AfaI GTAC 3 cut(s) 620, 1196, 1746
AfeI AGCGCT 2 cut(s) 755, 1283
AfiI CCNNNNNNNGG 2 cut(s) 66, 560
AflII CTTAAG 1 cut(s) 574
AflIII ACRYGT 2 cut(s) 1491, 1633
AgeI ACCGGT 1 cut(s) 162
AgsI TTSAA 7 cut(s) 10, 353, 782, 985, 1289, 1527, 1758
AhlI ACTAGT 2 cut(s) 286, 1370
AjiI CACGTC 1 cut(s) 1634
AjnI CCWGG 5 cut(s) 50, 414, 478, 649, 901
AluBI AGCT 5 cut(s) 14, 385, 1204, 1645, 1931
AluI AGCT 5 cut(s) 14, 385, 1204, 1645, 1931
Alw21I GWGCWC 3 cut(s) 264, 317, 1133
Alw26I GTCTC 2 cut(s) 993, 1437
AlwI GGATC 5 cut(s) 1355, 1562, 1604, 1617, 1865
Ama87I CYCGRG 1 cut(s) 1499
Aor13HI TCCGGA 1 cut(s) 1033
Aor51HI AGCGCT 2 cut(s) 755, 1283
AoxI GGCC 4 cut(s) 653, 775, 1579, 1662
ApeKI GCWGC 1 cut(s) 1049
ApoI RAATTY 4 cut(s) 151, 174, 608, 1407
AseI ATTAAT 1 cut(s) 870
AsiGI ACCGGT 1 cut(s) 162
Asp700I GAANNNNTTC 4 cut(s) 612, 1108, 1852, 1894
AspLEI GCGC 4 cut(s) 748, 756, 1284, 1735
AspS9I GGNCC 4 cut(s) 653, 1260, 1662, 1762
AsuC2I CCSGG 1 cut(s) 147
AsuHPI GGTGA 6 cut(s) 508, 651, 1004, 1319, 1488, 1814
AsuII TTCGAA 1 cut(s) 126
AvaI CYCGRG 1 cut(s) 1499
AvaII GGWCC 2 cut(s) 1260, 1762
BaeI ACNNNNGTAYC 2 cut(s) 180, 213
BamHI GGATCC 1 cut(s) 1609
BanI GGYRCC 1 cut(s) 839
BarI GAAGNNNNNNTAC 2 cut(s) 779, 811
BauI CACGAG 2 cut(s) 749, 1308
BbsI GAAGAC 1 cut(s) 798
Bbv12I GWGCWC 3 cut(s) 264, 317, 1133
BbvI GCAGC 1 cut(s) 1061
BccI CCATC 6 cut(s) 86, 248, 664, 764, 989, 1174
BciT130I CCWGG 5 cut(s) 52, 416, 480, 651, 903
BclI TGATCA 1 cut(s) 454
BcnI CCSGG 1 cut(s) 147
BcoDI GTCTC 2 cut(s) 993, 1437
BcuI ACTAGT 2 cut(s) 286, 1370
BfaI CTAG 9 cut(s) 156, 192, 287, 404, 663, 1244, 1371, 1385, 1428
BfoI RGCGCY 2 cut(s) 757, 1285
BfrI CTTAAG 1 cut(s) 574
BfuAI ACCTGC 1 cut(s) 1777
BglII AGATCT 2 cut(s) 1213, 1360
BisI GCNGC 2 cut(s) 1050, 1579
BlsI GCNGC 2 cut(s) 1051, 1580
Bme1390I CCNGG 6 cut(s) 52, 147, 416, 480, 651, 903
Bme18I GGWCC 2 cut(s) 1260, 1762
BmeT110I CYCGRG 1 cut(s) 1499
BmgBI CACGTC 1 cut(s) 1634
BmgT120I GGNCC 4 cut(s) 653, 1260, 1662, 1762
BmiI GGNNCC 3 cut(s) 71, 841, 1611
BmrFI CCNGG 6 cut(s) 52, 147, 416, 480, 651, 903
BmrI ACTGGG 1 cut(s) 519
BmsI GCATC 5 cut(s) 608, 1378, 1709, 1817, 1837
BmuI ACTGGG 1 cut(s) 519
BpiI GAAGAC 1 cut(s) 798
Bpu10I CCTNAGC 1 cut(s) 1824
Bpu14I TTCGAA 1 cut(s) 126
BpuEI CTTGAG 1 cut(s) 1321
BpuMI CCSGG 1 cut(s) 147
Bsa29I ATCGAT 1 cut(s) 1043
BsaBI GATNNNNATC 2 cut(s) 933, 1869
BsaI GGTCTC 1 cut(s) 1437
BsaJI CCNNGG 5 cut(s) 51, 145, 650, 902, 1438
BsaWI WCCGGW 2 cut(s) 162, 1033
BsaXI ACNNNNNCTCC 4 cut(s) 90, 120, 273, 303
Bsc4I CCNNNNNNNGG 2 cut(s) 66, 560
Bse118I RCCGGY 1 cut(s) 162
Bse1I ACTGG 2 cut(s) 514, 827
Bse3DI GCAATG 1 cut(s) 1817
Bse8I GATNNNNATC 2 cut(s) 933, 1869
BseAI TCCGGA 1 cut(s) 1033
BseBI CCWGG 5 cut(s) 52, 416, 480, 651, 903
BseCI ATCGAT 1 cut(s) 1043
BseDI CCNNGG 5 cut(s) 51, 145, 650, 902, 1438
BseGI GGATG 9 cut(s) 94, 424, 457, 599, 709, 772, 962, 1350, 1828
BseJI GATNNNNATC 2 cut(s) 933, 1869
BseLI CCNNNNNNNGG 2 cut(s) 66, 560
BseMI GCAATG 1 cut(s) 1817
BseMII CTCAG 2 cut(s) 200, 1383
BseNI ACTGG 2 cut(s) 514, 827
BseRI GAGGAG 2 cut(s) 20, 1803
BseX3I CGGCCG 1 cut(s) 1579
BseXI GCAGC 1 cut(s) 1061
BsgI GTGCAG 1 cut(s) 363
Bsh1236I CGCG 1 cut(s) 343
Bsh1285I CGRYCG 2 cut(s) 347, 1582
BshFI GGCC 4 cut(s) 655, 777, 1581, 1664
BshNI GGYRCC 1 cut(s) 839
BshTI ACCGGT 1 cut(s) 162
BshVI ATCGAT 1 cut(s) 1043
BsiEI CGRYCG 2 cut(s) 347, 1582
BsiHKAI GWGCWC 3 cut(s) 264, 317, 1133
BsiHKCI CYCGRG 1 cut(s) 1499
BsiSI CCGG 5 cut(s) 147, 163, 1034, 1582, 1832
BslFI GGGAC 4 cut(s) 68, 578, 796, 1159
BslI CCNNNNNNNGG 2 cut(s) 66, 560
BsmAI GTCTC 2 cut(s) 993, 1437
BsmBI CGTCTC 1 cut(s) 993
BsmFI GGGAC 4 cut(s) 68, 578, 796, 1159
BsmI GAATGC 1 cut(s) 1237
BsnI GGCC 4 cut(s) 655, 777, 1581, 1664
Bso31I GGTCTC 1 cut(s) 1437
BsoBI CYCGRG 1 cut(s) 1499
Bsp119I TTCGAA 1 cut(s) 126
Bsp1286I GDGCHC 3 cut(s) 264, 317, 1133
Bsp13I TCCGGA 1 cut(s) 1033
Bsp1407I TGTACA 3 cut(s) 618, 1194, 1744
Bsp143I GATC 7 cut(s) 454, 1213, 1347, 1360, 1554, 1609, 1870
Bsp19I CCATGG 1 cut(s) 1438
BspACI CCGC 2 cut(s) 343, 1578
BspANI GGCC 4 cut(s) 655, 777, 1581, 1664
BspCNI CTCAG 2 cut(s) 199, 1384
BspDI ATCGAT 1 cut(s) 1043
BspEI TCCGGA 1 cut(s) 1033
BspFNI CGCG 1 cut(s) 343
BspHI TCATGA 1 cut(s) 1713
BspLI GGNNCC 3 cut(s) 71, 841, 1611
BspMI ACCTGC 1 cut(s) 1777
BspPI GGATC 5 cut(s) 1355, 1562, 1604, 1617, 1865
BspT104I TTCGAA 1 cut(s) 126
BspT107I GGYRCC 1 cut(s) 839
BspTI CTTAAG 1 cut(s) 574
BspTNI GGTCTC 1 cut(s) 1437
BsrDI GCAATG 1 cut(s) 1817
BsrFI RCCGGY 1 cut(s) 162
BsrGI TGTACA 3 cut(s) 618, 1194, 1744
BsrI ACTGG 2 cut(s) 514, 827
BssAI RCCGGY 1 cut(s) 162
BssECI CCNNGG 5 cut(s) 51, 145, 650, 902, 1438
BssMI GATC 7 cut(s) 454, 1213, 1347, 1360, 1554, 1609, 1870
BssSI CACGAG 2 cut(s) 749, 1308
BssT1I CCWWGG 1 cut(s) 1438
Bst2BI CACGAG 2 cut(s) 749, 1308
Bst2UI CCWGG 5 cut(s) 52, 416, 480, 651, 903
Bst4CI ACNGT 6 cut(s) 211, 733, 834, 1150, 1240, 1260
Bst6I CTCTTC 3 cut(s) 29, 38, 1843
BstAFI CTTAAG 1 cut(s) 574
BstAUI TGTACA 3 cut(s) 618, 1194, 1744
BstBI TTCGAA 1 cut(s) 126
BstDEI CTNAG 5 cut(s) 186, 1100, 1392, 1666, 1824
BstDSI CCRYGG 1 cut(s) 1438
BstF5I GGATG 9 cut(s) 94, 424, 457, 599, 709, 772, 962, 1350, 1828
BstFNI CGCG 1 cut(s) 343
BstH2I RGCGCY 2 cut(s) 757, 1285
BstHHI GCGC 4 cut(s) 748, 756, 1284, 1735
BstKTI GATC 7 cut(s) 457, 1216, 1350, 1363, 1557, 1612, 1873
BstMAI GTCTC 2 cut(s) 993, 1437
BstMBI GATC 7 cut(s) 454, 1213, 1347, 1360, 1554, 1609, 1870
BstMCI CGRYCG 2 cut(s) 347, 1582
BstMWI GCNNNNNNNGC 2 cut(s) 855, 1778
BstNI CCWGG 5 cut(s) 52, 416, 480, 651, 903
BstNSI RCATGY 2 cut(s) 1495, 1883
BstSCI CCNGG 6 cut(s) 50, 145, 414, 478, 649, 901
BstUI CGCG 1 cut(s) 343
BstV1I GCAGC 1 cut(s) 1061
BstV2I GAAGAC 1 cut(s) 798
BstX2I RGATCY 3 cut(s) 1213, 1360, 1609
BstYI RGATCY 3 cut(s) 1213, 1360, 1609
BstZI CGGCCG 1 cut(s) 1579
Bsu15I ATCGAT 1 cut(s) 1043
BsuRI GGCC 4 cut(s) 655, 777, 1581, 1664
BsuTUI ATCGAT 1 cut(s) 1043
BtgI CCRYGG 1 cut(s) 1438
BtrI CACGTC 1 cut(s) 1634
BtsCI GGATG 9 cut(s) 94, 424, 457, 599, 709, 772, 962, 1350, 1828
BtsI GCAGTG 1 cut(s) 1116
BtsIMutI CAGTG 5 cut(s) 216, 839, 1116, 1131, 1685
BveI ACCTGC 1 cut(s) 1777
CciI TCATGA 1 cut(s) 1713
CfoI GCGC 4 cut(s) 748, 756, 1284, 1735
Cfr10I RCCGGY 1 cut(s) 162
Cfr13I GGNCC 4 cut(s) 653, 1260, 1662, 1762
ClaI ATCGAT 1 cut(s) 1043
CseI GACGC 1 cut(s) 1868
Csp6I GTAC 3 cut(s) 619, 1195, 1745
CspAI ACCGGT 1 cut(s) 162
CviQI GTAC 3 cut(s) 619, 1195, 1745
DdeI CTNAG 5 cut(s) 186, 1100, 1392, 1666, 1824
DpnI GATC 7 cut(s) 456, 1215, 1349, 1362, 1556, 1611, 1872
DpnII GATC 7 cut(s) 454, 1213, 1347, 1360, 1554, 1609, 1870
DraIII CACNNNGTG 1 cut(s) 665
DrdI GACNNNNNNGTC 1 cut(s) 1690
DseDI GACNNNNNNGTC 1 cut(s) 1690
EaeI YGGCCR 1 cut(s) 1579
EagI CGGCCG 1 cut(s) 1579
Eam1104I CTCTTC 3 cut(s) 29, 38, 1843
EarI CTCTTC 3 cut(s) 29, 38, 1843
EclXI CGGCCG 1 cut(s) 1579
Eco130I CCWWGG 1 cut(s) 1438
Eco31I GGTCTC 1 cut(s) 1437
Eco47I GGWCC 2 cut(s) 1260, 1762
Eco47III AGCGCT 2 cut(s) 755, 1283
Eco52I CGGCCG 1 cut(s) 1579
Eco57I CTGAAG 3 cut(s) 104, 124, 1709
Eco88I CYCGRG 1 cut(s) 1499
EcoO109I RGGNCCY 1 cut(s) 1662
EcoRII CCWGG 5 cut(s) 50, 414, 478, 649, 901
EcoT14I CCWWGG 1 cut(s) 1438
EcoT22I ATGCAT 1 cut(s) 1724
ErhI CCWWGG 1 cut(s) 1438
Esp3I CGTCTC 1 cut(s) 993
FalI AAGNNNNNCTT 1 cut(s) 35
FaqI GGGAC 4 cut(s) 68, 578, 796, 1159
FauNDI CATATG 1 cut(s) 1483
FbaI TGATCA 1 cut(s) 454
FblI GTMKAC 1 cut(s) 329
Fnu4HI GCNGC 2 cut(s) 1050, 1579
FokI GGATG 9 cut(s) 101, 431, 444, 586, 716, 779, 969, 1337, 1815
Fsp4HI GCNGC 2 cut(s) 1050, 1579
FspBI CTAG 9 cut(s) 156, 192, 287, 404, 663, 1244, 1371, 1385, 1428
FspI TGCGCA 1 cut(s) 747
GlaI GCGC 4 cut(s) 747, 755, 1283, 1734
GluI GCNGC 2 cut(s) 1050, 1579
HaeII RGCGCY 2 cut(s) 757, 1285
HaeIII GGCC 4 cut(s) 655, 777, 1581, 1664
HapII CCGG 5 cut(s) 147, 163, 1034, 1582, 1832
HgaI GACGC 1 cut(s) 1868
HhaI GCGC 4 cut(s) 748, 756, 1284, 1735
Hin6I GCGC 4 cut(s) 746, 754, 1282, 1733
HinP1I GCGC 4 cut(s) 746, 754, 1282, 1733
HinfI GANTC 8 cut(s) 47, 740, 934, 1334, 1679, 1754, 1853, 1895
HpaII CCGG 5 cut(s) 147, 163, 1034, 1582, 1832
HphI GGTGA 6 cut(s) 508, 651, 1004, 1319, 1488, 1814
Hpy166II GTNNAC 6 cut(s) 330, 899, 1195, 1197, 1745, 1843
Hpy188III TCNNGA 6 cut(s) 680, 1034, 1065, 1338, 1385, 1714
Hpy8I GTNNAC 6 cut(s) 330, 899, 1195, 1197, 1745, 1843
Hpy99I CGWCG 1 cut(s) 1862
HpyAV CCTTC 8 cut(s) 79, 781, 788, 1006, 1136, 1273, 1623, 1649
HpyCH4III ACNGT 6 cut(s) 211, 733, 834, 1150, 1240, 1260
HpyCH4IV ACGT 2 cut(s) 1221, 1633
HpyF10VI GCNNNNNNNGC 2 cut(s) 855, 1778
HpyF3I CTNAG 5 cut(s) 186, 1100, 1392, 1666, 1824
HpySE526I ACGT 2 cut(s) 1221, 1633
HspAI GCGC 4 cut(s) 746, 754, 1282, 1733
Kpn2I TCCGGA 1 cut(s) 1033
Ksp22I TGATCA 1 cut(s) 454
Kzo9I GATC 7 cut(s) 454, 1213, 1347, 1360, 1554, 1609, 1870
LmnI GCTCC 1 cut(s) 1642
Lsp1109I GCAGC 1 cut(s) 1061
LweI GCATC 5 cut(s) 608, 1378, 1709, 1817, 1837
MaeI CTAG 9 cut(s) 156, 192, 287, 404, 663, 1244, 1371, 1385, 1428
MaeII ACGT 2 cut(s) 1221, 1633
MaeIII GTNAC 6 cut(s) 540, 670, 733, 818, 1073, 1494
MalI GATC 7 cut(s) 456, 1215, 1349, 1362, 1556, 1611, 1872
MboI GATC 7 cut(s) 454, 1213, 1347, 1360, 1554, 1609, 1870
MboII GAAGA 8 cut(s) 16, 25, 130, 803, 1316, 1373, 1763, 1860
MfeI CAATTG 2 cut(s) 558, 1448
MflI RGATCY 3 cut(s) 1213, 1360, 1609
MhlI GDGCHC 3 cut(s) 264, 317, 1133
MlyI GAGTC 1 cut(s) 1328
MmeI TCCRAC 5 cut(s) 61, 151, 1456, 1545, 1881
MnlI CCTC 7 cut(s) 38, 41, 262, 676, 1338, 1600, 1824
Mph1103I ATGCAT 1 cut(s) 1724
MroI TCCGGA 1 cut(s) 1033
MroXI GAANNNNTTC 4 cut(s) 612, 1108, 1852, 1894
MseI TTAA 7 cut(s) 360, 473, 527, 575, 870, 1026, 1058
MslI CAYNNNNRTG 1 cut(s) 1190
MspCI CTTAAG 1 cut(s) 574
MspI CCGG 5 cut(s) 147, 163, 1034, 1582, 1832
MspR9I CCNGG 6 cut(s) 52, 147, 416, 480, 651, 903
MunI CAATTG 2 cut(s) 558, 1448
Mva1269I GAATGC 1 cut(s) 1237
MvaI CCWGG 5 cut(s) 52, 416, 480, 651, 903
MvnI CGCG 1 cut(s) 343
MwoI GCNNNNNNNGC 2 cut(s) 855, 1778
NciI CCSGG 1 cut(s) 147
NcoI CCATGG 1 cut(s) 1438
NdeI CATATG 1 cut(s) 1483
NdeII GATC 7 cut(s) 454, 1213, 1347, 1360, 1554, 1609, 1870
NlaIV GGNNCC 3 cut(s) 71, 841, 1611
NmuCI GTSAC 6 cut(s) 540, 670, 733, 818, 1073, 1494
NsbI TGCGCA 1 cut(s) 747
NsiI ATGCAT 1 cut(s) 1724
NspI RCATGY 2 cut(s) 1495, 1883
NspV TTCGAA 1 cut(s) 126
PagI TCATGA 1 cut(s) 1713
PciI ACATGT 1 cut(s) 1491
PctI GAATGC 1 cut(s) 1237
PdmI GAANNNNTTC 4 cut(s) 612, 1108, 1852, 1894
PfeI GAWTC 7 cut(s) 47, 740, 934, 1679, 1754, 1853, 1895
PflMI CCANNNNNTGG 1 cut(s) 66
PfoI TCCNGGA 1 cut(s) 414
PinAI ACCGGT 1 cut(s) 162
PkrI GCNGC 2 cut(s) 1051, 1580
PleI GAGTC 1 cut(s) 1328
PpsI GAGTC 1 cut(s) 1328
PscI ACATGT 1 cut(s) 1491
PshBI ATTAAT 1 cut(s) 870
PsiI TTATAA 1 cut(s) 303
Psp6I CCWGG 5 cut(s) 50, 414, 478, 649, 901
PspGI CCWGG 5 cut(s) 50, 414, 478, 649, 901
PspN4I GGNNCC 3 cut(s) 71, 841, 1611
PspPI GGNCC 4 cut(s) 653, 1260, 1662, 1762
PsuI RGATCY 3 cut(s) 1213, 1360, 1609
RsaI GTAC 3 cut(s) 620, 1196, 1746
RsaNI GTAC 3 cut(s) 619, 1195, 1745
RseI CAYNNNNRTG 1 cut(s) 1190
SaqAI TTAA 7 cut(s) 360, 473, 527, 575, 870, 1026, 1058
SatI GCNGC 2 cut(s) 1050, 1579
Sau3AI GATC 7 cut(s) 454, 1213, 1347, 1360, 1554, 1609, 1870
Sau96I GGNCC 4 cut(s) 653, 1260, 1662, 1762
SchI GAGTC 1 cut(s) 1328
ScrFI CCNGG 6 cut(s) 52, 147, 416, 480, 651, 903
SduI GDGCHC 3 cut(s) 264, 317, 1133
SfaNI GCATC 5 cut(s) 608, 1378, 1709, 1817, 1837
SfuI TTCGAA 1 cut(s) 126
SinI GGWCC 2 cut(s) 1260, 1762
SmiMI CAYNNNNRTG 1 cut(s) 1190
SmlI CTYRAG 2 cut(s) 574, 1336
SmoI CTYRAG 2 cut(s) 574, 1336
SpeI ACTAGT 2 cut(s) 286, 1370
SsiI CCGC 2 cut(s) 343, 1578
SspI AATATT 3 cut(s) 358, 1378, 1434
SspMI CTAG 9 cut(s) 156, 192, 287, 404, 663, 1244, 1371, 1385, 1428
StyD4I CCNGG 6 cut(s) 50, 145, 414, 478, 649, 901
StyI CCWWGG 1 cut(s) 1438
TaaI ACNGT 6 cut(s) 211, 733, 834, 1150, 1240, 1260
TaiI ACGT 2 cut(s) 1224, 1636
TaqI TCGA 5 cut(s) 126, 970, 1043, 1682, 1933
TaqII GACCGA 1 cut(s) 1779
TatI WGTACW 3 cut(s) 618, 1194, 1744
TauI GCSGC 1 cut(s) 1581
TfiI GAWTC 7 cut(s) 47, 740, 934, 1679, 1754, 1853, 1895
Tru1I TTAA 7 cut(s) 360, 473, 527, 575, 870, 1026, 1058
Tru9I TTAA 7 cut(s) 360, 473, 527, 575, 870, 1026, 1058
TscAI CASTG 5 cut(s) 216, 839, 1123, 1138, 1692
TseFI GTSAC 6 cut(s) 540, 670, 733, 818, 1073, 1494
TseI GCWGC 1 cut(s) 1049
Tsp45I GTSAC 6 cut(s) 540, 670, 733, 818, 1073, 1494
TspDTI ATGAA 7 cut(s) 444, 500, 1155, 1412, 1634, 1722, 1780
TspGWI ACGGA 1 cut(s) 1902
TspRI CASTG 5 cut(s) 216, 839, 1123, 1138, 1692
Van91I CCANNNNNTGG 1 cut(s) 66
Vha464I CTTAAG 1 cut(s) 574
VpaK11BI GGWCC 2 cut(s) 1260, 1762
VspI ATTAAT 1 cut(s) 870
XapI RAATTY 4 cut(s) 151, 174, 608, 1407
XbaI TCTAGA 1 cut(s) 1384
XceI RCATGY 2 cut(s) 1495, 1883
XmiI GTMKAC 1 cut(s) 329
XmnI GAANNNNTTC 4 cut(s) 612, 1108, 1852, 1894
XspI CTAG 9 cut(s) 156, 192, 287, 404, 663, 1244, 1371, 1385, 1428
Zsp2I ATGCAT 1 cut(s) 1724
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.