FvH4_3g03390

divergent subfamily of APPLE domains

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000112
Physical Location & Seq
Forward (+)
1832450 .. 1838776
6327 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g03390.t7

Sequence Viewer

Length: 2229 bp
ATGTTGACTAGAAGGTATGGAAACTTGGTTCTTTATGCTTATAAGGAAAACTTAACTATTTGGTCCACAGAGGTGTCAGTGCAAACTACAAGTAGTACTTCAGCTCAAATTCTAGATACAGGAAACTTGGTTTTGGTCCAGGGTAACAATGAAGAATTTATATGGCAAAGCTTTGATTATCCAACGGATACTATGATTCCAGGAATGAAACTTGGGTTGAATCGTGAGACCGGTCTGGACAGCTTCATAACATCCTGGAAGTCAGAAGATGACCCTGGAACTGGGGACTATTCATACAGGCTCAACCTTACTGGCGCCCCACAATTCATTCTGTATAATCGTTCAAGTAAGTATTTTCGAACGGCTATGTGGCCATGGAGTCCATCACCAAGTACAACACCTTATGCATATGACTACAATTTTGTTAATAATCAAGATGAGACATCTTTGATTGATTCTTCTGATAAATCCTCCGAAATTTCAAGACTTGTGGTGAACCATAATGGCATACTTCAGCACCTAACATGGAATGATGTTGAGAGTCGATGGAAGGAAATGTGGGCGGCGCCAAAATTCAGGTGTGATCCGTATGGACATTGTGGCGCCTTCAGTGAATGTAGTCTTGACAATATACAATCCGAATGTTCATGTTTGCCAGGGTATGAACCAAGTTCTTCAGGAAATTGGTCAGAAGGGATTTGGTCAGATGGGTGTGTGAGTAAGCAGGTAGAAGTATCTAATAAGTGTGGAAAGAATTTTGTGAAGGTGGAACATGTGAAGCTTCCAGATGCATCAATATCAGCTTTGCTGGACACACAGATGGGTGAAAGTGAGTGCAAGAGAGCATGCCAGAACAATTGTTCATGCGTCGCATACGCAAGTCTACAAATTGATGGAGGTGGTGGTTGCATGGCATGGTATGGGGAATTGATGGACACTTTAATATTGGCAGAGCAGGGACGAGATTTCTATGTTCGAGCAGATGGAACTGAGGCAGCTGAGAATGACAGAAAACCAAGTTTCCTCAGAAGGAGGGGAATGCTGGCTGTTCTAATTTTACCTGCTGTGCTGGCACTTTCTGTCATCATGATGGCCTACTGGTGGCTTATCAAGAGAAAGACTGAAGCCATTATATTGAGAAGGACATGGGGGAGACAATACTCTCATTTTAGTACTTTCAATGAGCCTGTGGTGGAACACAAAGTAGGGGAAACTCGTAGACACCCAGATTTGCAACTTTTCTATCTCAGCACAATAAAAGCTGCAACAGATGACTTCTCTCTGGCTAATAAACTAGGAGAAGGTGGTTTTGGCTCTGTTTATAAGGGCCACTTATCCAACCAACAGAAGATTGCTGTGAAAAGATTGTCCAAGAGTTCAAGACAAGGAGTTGAAGAATTTAAAAACGAAGTTACACTCATTGCAAAACTTCAACACAGGAATCTTGTGAAGCTATTAGGATGTTGTATAGAGGAAGAAGAGAAGTTGTTAATCTATGAATACTTGCCAAACAAAAGTTTGGACTTCTTTCTTTTTGATCAAACTCGTAGGTCTGCCTTGGATTGGAGAAATCGATTTGCAATAATCACTGGAGTTGCTCGTGGGATTTTGTATCTCCATGAAGACTCAAGGTTGAGAATTGTCCACAGGGATCTGAAAGCTAGCAATATTTTGCTTGATGCTGACATGAAGCCGAAAATTTCAGATTTCGGCTTGGCTAGAATATTTGATGGCGACCAACTACAGGATGTGACACGAAAAATTGTAGGGACATATGGTTATATGTCACCAGAGTATGCAGGGTATGGAAAATTTTCCACAAAATCAGATGTGTTTAGCTTTGGGGTTATAGTGTTGGAGATTATAAGTGGCAAGAAAAACAATGCTTCTTATGAGGAGGACCATTCCATCAATTTGATAGGTCATGTATGGGACTTATGGAGAGAACACAGTATCCTAGACATTGTGGATGTCGTACTGGAGTCATATAACATTGATGAAGTGCTGAGATGCCTTCAAATTGGTCTCTTATGTCTGGAAGAAGATACAACAGATAGGCCAACTATGTCCGAAGTTGTCCTCATGTTGAGTGGTGAAAGAGCTCTTGCATCCCCTCAAAGACCAGCATTCATCTTCAAAAAAACTTGCCACAATAGTTCCGGTTTACTAATAACAGAAAGATTTTCTTCCACTAATGATATAACAGTTACTGATGTTGTAGGTCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

743

Amino Acids

83.98

Weight (kDa)

5.66

Isoelectric Point (pI)

49.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK_Tyr_Ser-Thr PF07714 82 - 347 8.7e-51 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 82 - 347 5.6e-48 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 42, 1323, 1865
Acc36I ACCTGC 2 cut(s) 715, 1069
AccB1I GGYRCC 3 cut(s) 314, 565, 602
AccI GTMKAC 2 cut(s) 883, 1219
AciI CCGC 1 cut(s) 563
AclWI GGATC 2 cut(s) 578, 1659
AcoI YGGCCR 1 cut(s) 371
AcsI RAATTY 8 cut(s) 108, 155, 477, 572, 754, 1397, 1698, 1811
AcuI CTGAAG 5 cut(s) 84, 497, 592, 660, 1143
AcyI GRCGYC 3 cut(s) 315, 566, 603
AfaI GTAC 4 cut(s) 97, 394, 1174, 1977
AfiI CCNNNNNNNGG 4 cut(s) 281, 1101, 1563, 1744
AflIII ACRYGT 1 cut(s) 772
AgeI ACCGGT 1 cut(s) 230
AgsI TTSAA 9 cut(s) 220, 345, 483, 1180, 1380, 1394, 1433, 2018, 2137
AjnI CCWGG 5 cut(s) 138, 199, 254, 274, 655
AjuI GAANNNNNNNTTGG 2 cut(s) 1293, 1325
AleI CACNNNNGTG 1 cut(s) 71
AloI GAACNNNNNNTCC 4 cut(s) 12, 44, 1938, 1970
Alw21I GWGCWC 1 cut(s) 2104
Alw26I GTCTC 4 cut(s) 221, 434, 1147, 2030
AlwI GGATC 2 cut(s) 578, 1659
AlwNI CAGNNNCTG 1 cut(s) 2210
AoxI GGCC 4 cut(s) 371, 1092, 1327, 2057
ApeKI GCWGC 2 cut(s) 995, 1262
ApoI RAATTY 8 cut(s) 108, 155, 477, 572, 754, 1397, 1698, 1811
ArsI GACNNNNNNTTYG 2 cut(s) 404, 436
AsiGI ACCGGT 1 cut(s) 230
Asp700I GAANNNNTTC 1 cut(s) 1813
AspLEI GCGC 3 cut(s) 317, 568, 605
AspS9I GGNCC 4 cut(s) 63, 136, 1327, 1900
AsuHPI GGTGA 5 cut(s) 378, 505, 836, 1779, 2105
AsuII TTCGAA 1 cut(s) 358
AsuNHI GCTAGC 1 cut(s) 1661
AvaII GGWCC 3 cut(s) 63, 136, 1900
BalI TGGCCA 1 cut(s) 373
BanI GGYRCC 3 cut(s) 314, 565, 602
BanII GRGCYC 1 cut(s) 2104
BauI CACGAG 1 cut(s) 1599
BbsI GAAGAC 1 cut(s) 1629
Bbv12I GWGCWC 1 cut(s) 2104
BbvI GCAGC 2 cut(s) 1007, 1249
BceAI ACGGC 1 cut(s) 378
BciT130I CCWGG 5 cut(s) 140, 201, 256, 276, 657
BciVI GTATCC 2 cut(s) 181, 1964
BclI TGATCA 1 cut(s) 1537
BcoDI GTCTC 4 cut(s) 221, 434, 1147, 2030
BfaI CTAG 6 cut(s) 9, 113, 1295, 1662, 1719, 1958
BfmI CTRYAG 1 cut(s) 1742
BfoI RGCGCY 3 cut(s) 318, 569, 606
BfuAI ACCTGC 2 cut(s) 715, 1069
BfuI GTATCC 2 cut(s) 181, 1964
BisI GCNGC 3 cut(s) 564, 996, 1263
BlsI GCNGC 3 cut(s) 565, 997, 1264
BmcAI AGTACT 2 cut(s) 97, 1174
Bme1390I CCNGG 5 cut(s) 140, 201, 256, 276, 657
Bme18I GGWCC 3 cut(s) 63, 136, 1900
BmgT120I GGNCC 4 cut(s) 63, 136, 1327, 1900
BmiI GGNNCC 3 cut(s) 316, 567, 604
BmrFI CCNGG 5 cut(s) 140, 201, 256, 276, 657
BmrI ACTGGG 1 cut(s) 291
BmsI GCATC 5 cut(s) 778, 800, 1669, 2000, 2117
BmtI GCTAGC 1 cut(s) 1665
BmuI ACTGGG 1 cut(s) 291
BpiI GAAGAC 1 cut(s) 1629
BpmI CTGGAG 2 cut(s) 1611, 2000
Bpu14I TTCGAA 1 cut(s) 358
BpuEI CTTGAG 1 cut(s) 1612
Bsa29I ATCGAT 1 cut(s) 1573
BsaHI GRCGYC 3 cut(s) 315, 566, 603
BsaI GGTCTC 2 cut(s) 221, 2030
BsaJI CCNNGG 5 cut(s) 139, 274, 374, 656, 1557
BsaWI WCCGGW 2 cut(s) 230, 2159
Bsc4I CCNNNNNNNGG 4 cut(s) 281, 1101, 1563, 1744
Bse118I RCCGGY 1 cut(s) 230
Bse1I ACTGG 5 cut(s) 286, 316, 1103, 1594, 1983
Bse3DI GCAATG 1 cut(s) 1419
BseBI CCWGG 5 cut(s) 140, 201, 256, 276, 657
BseCI ATCGAT 1 cut(s) 1573
BseDI CCNNGG 5 cut(s) 139, 274, 374, 656, 1557
BseGI GGATG 5 cut(s) 251, 1466, 1753, 1975, 2108
BseLI CCNNNNNNNGG 4 cut(s) 281, 1101, 1563, 1744
BseMI GCAATG 1 cut(s) 1419
BseMII CTCAG 5 cut(s) 981, 990, 1039, 1261, 1997
BseNI ACTGG 5 cut(s) 286, 316, 1103, 1594, 1983
BseRI GAGGAG 1 cut(s) 1910
BseXI GCAGC 2 cut(s) 1007, 1249
BshFI GGCC 4 cut(s) 373, 1094, 1329, 2059
BshNI GGYRCC 3 cut(s) 314, 565, 602
BshTI ACCGGT 1 cut(s) 230
BshVI ATCGAT 1 cut(s) 1573
BsiHKAI GWGCWC 1 cut(s) 2104
BsiSI CCGG 2 cut(s) 231, 2160
BslFI GGGAC 4 cut(s) 299, 972, 1783, 1946
BslI CCNNNNNNNGG 4 cut(s) 281, 1101, 1563, 1744
BsmAI GTCTC 4 cut(s) 221, 434, 1147, 2030
BsmFI GGGAC 4 cut(s) 299, 972, 1783, 1946
BsmI GAATGC 2 cut(s) 1044, 2126
BsnI GGCC 4 cut(s) 373, 1094, 1329, 2059
Bso31I GGTCTC 2 cut(s) 221, 2030
Bsp119I TTCGAA 1 cut(s) 358
Bsp1286I GDGCHC 1 cut(s) 2104
Bsp143I GATC 3 cut(s) 583, 1537, 1651
Bsp19I CCATGG 1 cut(s) 374
BspACI CCGC 1 cut(s) 563
BspANI GGCC 4 cut(s) 373, 1094, 1329, 2059
BspCNI CTCAG 5 cut(s) 982, 991, 1038, 1260, 1998
BspDI ATCGAT 1 cut(s) 1573
BspHI TCATGA 1 cut(s) 1086
BspLI GGNNCC 3 cut(s) 316, 567, 604
BspMI ACCTGC 2 cut(s) 715, 1069
BspOI GCTAGC 1 cut(s) 1665
BspPI GGATC 2 cut(s) 578, 1659
BspT104I TTCGAA 1 cut(s) 358
BspT107I GGYRCC 3 cut(s) 314, 565, 602
BspTNI GGTCTC 2 cut(s) 221, 2030
BsrDI GCAATG 1 cut(s) 1419
BsrFI RCCGGY 1 cut(s) 230
BsrI ACTGG 5 cut(s) 286, 316, 1103, 1594, 1983
BssAI RCCGGY 1 cut(s) 230
BssECI CCNNGG 5 cut(s) 139, 274, 374, 656, 1557
BssMI GATC 3 cut(s) 583, 1537, 1651
BssNI GRCGYC 3 cut(s) 315, 566, 603
BssSI CACGAG 1 cut(s) 1599
BssT1I CCWWGG 2 cut(s) 374, 1557
Bst2BI CACGAG 1 cut(s) 1599
Bst2UI CCWGG 5 cut(s) 140, 201, 256, 276, 657
Bst4CI ACNGT 2 cut(s) 1952, 2206
Bst6I CTCTTC 1 cut(s) 1473
BstACI GRCGYC 3 cut(s) 315, 566, 603
BstBI TTCGAA 1 cut(s) 358
BstC8I GCNNGC 4 cut(s) 847, 1044, 1071, 1663
BstDEI CTNAG 5 cut(s) 990, 999, 1025, 1247, 2006
BstDSI CCRYGG 1 cut(s) 374
BstF5I GGATG 5 cut(s) 251, 1466, 1753, 1975, 2108
BstH2I RGCGCY 3 cut(s) 318, 569, 606
BstHHI GCGC 3 cut(s) 317, 568, 605
BstKTI GATC 3 cut(s) 586, 1540, 1654
BstMAI GTCTC 4 cut(s) 221, 434, 1147, 2030
BstMBI GATC 3 cut(s) 583, 1537, 1651
BstMWI GCNNNNNNNGC 1 cut(s) 1070
BstNI CCWGG 5 cut(s) 140, 201, 256, 276, 657
BstNSI RCATGY 2 cut(s) 776, 849
BstSCI CCNGG 5 cut(s) 138, 199, 254, 274, 655
BstSFI CTRYAG 1 cut(s) 1742
BstV1I GCAGC 2 cut(s) 1007, 1249
BstV2I GAAGAC 1 cut(s) 1629
BstX2I RGATCY 1 cut(s) 1651
BstYI RGATCY 1 cut(s) 1651
Bsu15I ATCGAT 1 cut(s) 1573
BsuI GTATCC 2 cut(s) 181, 1964
BsuRI GGCC 4 cut(s) 373, 1094, 1329, 2059
BsuTUI ATCGAT 1 cut(s) 1573
BtgI CCRYGG 1 cut(s) 374
BtsCI GGATG 5 cut(s) 251, 1466, 1753, 1975, 2108
BtsIMutI CAGTG 3 cut(s) 84, 616, 1587
BveI ACCTGC 2 cut(s) 715, 1069
Cac8I GCNNGC 4 cut(s) 847, 1044, 1071, 1663
CaiI CAGNNNCTG 1 cut(s) 2210
CciI TCATGA 1 cut(s) 1086
CfoI GCGC 3 cut(s) 317, 568, 605
Cfr10I RCCGGY 1 cut(s) 230
Cfr13I GGNCC 4 cut(s) 63, 136, 1327, 1900
ClaI ATCGAT 1 cut(s) 1573
CseI GACGC 1 cut(s) 856
Csp6I GTAC 4 cut(s) 96, 393, 1173, 1976
CspAI ACCGGT 1 cut(s) 230
CspCI CAANNNNNGTGG 2 cut(s) 471, 506
CviQI GTAC 4 cut(s) 96, 393, 1173, 1976
DdeI CTNAG 5 cut(s) 990, 999, 1025, 1247, 2006
DinI GGCGCC 3 cut(s) 316, 567, 604
DpnI GATC 3 cut(s) 585, 1539, 1653
DpnII GATC 3 cut(s) 583, 1537, 1651
DraI TTTAAA 1 cut(s) 1402
EaeI YGGCCR 1 cut(s) 371
Eam1104I CTCTTC 1 cut(s) 1473
EarI CTCTTC 1 cut(s) 1473
Ecl136II GAGCTC 1 cut(s) 2102
Eco130I CCWWGG 2 cut(s) 374, 1557
Eco24I GRGCYC 1 cut(s) 2104
Eco31I GGTCTC 2 cut(s) 221, 2030
Eco47I GGWCC 3 cut(s) 63, 136, 1900
Eco53kI GAGCTC 1 cut(s) 2102
Eco57I CTGAAG 5 cut(s) 84, 497, 592, 660, 1143
EcoICRI GAGCTC 1 cut(s) 2102
EcoRII CCWGG 5 cut(s) 138, 199, 254, 274, 655
EcoT14I CCWWGG 2 cut(s) 374, 1557
EcoT22I ATGCAT 2 cut(s) 409, 793
EcoT38I GRGCYC 1 cut(s) 2104
EgeI GGCGCC 3 cut(s) 316, 567, 604
EheI GGCGCC 3 cut(s) 316, 567, 604
ErhI CCWWGG 2 cut(s) 374, 1557
FalI AAGNNNNNCTT 8 cut(s) 35, 67, 82, 114, 1316, 1348, 2170, 2202
FaqI GGGAC 4 cut(s) 299, 972, 1783, 1946
FauNDI CATATG 2 cut(s) 409, 1774
FbaI TGATCA 1 cut(s) 1537
FblI GTMKAC 2 cut(s) 883, 1219
Fnu4HI GCNGC 3 cut(s) 564, 996, 1263
FokI GGATG 5 cut(s) 238, 1473, 1760, 1982, 2095
FriOI GRGCYC 1 cut(s) 2104
Fsp4HI GCNGC 3 cut(s) 564, 996, 1263
FspBI CTAG 6 cut(s) 9, 113, 1295, 1662, 1719, 1958
GlaI GCGC 3 cut(s) 316, 567, 604
GluI GCNGC 3 cut(s) 564, 996, 1263
GsuI CTGGAG 2 cut(s) 1611, 2000
HaeII RGCGCY 3 cut(s) 318, 569, 606
HaeIII GGCC 4 cut(s) 373, 1094, 1329, 2059
HapII CCGG 2 cut(s) 231, 2160
HgaI GACGC 1 cut(s) 856
HhaI GCGC 3 cut(s) 317, 568, 605
Hin1I GRCGYC 3 cut(s) 315, 566, 603
Hin6I GCGC 3 cut(s) 315, 566, 603
HinP1I GCGC 3 cut(s) 315, 566, 603
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HindIII AAGCTT 2 cut(s) 169, 779
HinfI GANTC 8 cut(s) 196, 220, 379, 455, 541, 1441, 1625, 1982
HpaII CCGG 2 cut(s) 231, 2160
HphI GGTGA 5 cut(s) 378, 505, 836, 1779, 2105
Hpy166II GTNNAC 7 cut(s) 6, 66, 496, 884, 1220, 1645, 2165
Hpy8I GTNNAC 7 cut(s) 6, 66, 496, 884, 1220, 1645, 2165
Hpy99I CGWCG 1 cut(s) 872
HpyAV CCTTC 9 cut(s) 6, 544, 616, 686, 757, 1023, 1134, 1295, 2024
HpyCH4III ACNGT 2 cut(s) 1952, 2206
HpyF10VI GCNNNNNNNGC 1 cut(s) 1070
HpyF3I CTNAG 5 cut(s) 990, 999, 1025, 1247, 2006
Hsp92I GRCGYC 3 cut(s) 315, 566, 603
HspAI GCGC 3 cut(s) 315, 566, 603
KasI GGCGCC 3 cut(s) 314, 565, 602
Ksp22I TGATCA 1 cut(s) 1537
Kzo9I GATC 3 cut(s) 583, 1537, 1651
Lsp1109I GCAGC 2 cut(s) 1007, 1249
LweI GCATC 5 cut(s) 778, 800, 1669, 2000, 2117
MaeI CTAG 6 cut(s) 9, 113, 1295, 1662, 1719, 1958
MaeIII GTNAC 5 cut(s) 143, 1411, 1750, 1785, 2206
MalI GATC 3 cut(s) 585, 1539, 1653
MboI GATC 3 cut(s) 583, 1537, 1651
MfeI CAATTG 1 cut(s) 856
MflI RGATCY 1 cut(s) 1651
MhlI GDGCHC 1 cut(s) 2104
MlsI TGGCCA 1 cut(s) 373
MluNI TGGCCA 1 cut(s) 373
Mly113I GGCGCC 3 cut(s) 315, 566, 603
MlyI GAGTC 4 cut(s) 388, 550, 1619, 1991
MmeI TCCRAC 3 cut(s) 206, 1362, 1836
Mox20I TGGCCA 1 cut(s) 373
Mph1103I ATGCAT 2 cut(s) 409, 793
MroXI GAANNNNTTC 1 cut(s) 1813
MscI TGGCCA 1 cut(s) 373
MseI TTAA 5 cut(s) 53, 426, 941, 1401, 1490
MslI CAYNNNNRTG 3 cut(s) 71, 818, 1088
Msp20I TGGCCA 1 cut(s) 373
MspA1I CMGCKG 1 cut(s) 998
MspI CCGG 2 cut(s) 231, 2160
MspR9I CCNGG 5 cut(s) 140, 201, 256, 276, 657
MunI CAATTG 1 cut(s) 856
Mva1269I GAATGC 2 cut(s) 1044, 2126
MvaI CCWGG 5 cut(s) 140, 201, 256, 276, 657
MwoI GCNNNNNNNGC 1 cut(s) 1070
NarI GGCGCC 3 cut(s) 315, 566, 603
NcoI CCATGG 1 cut(s) 374
NdeI CATATG 2 cut(s) 409, 1774
NdeII GATC 3 cut(s) 583, 1537, 1651
NheI GCTAGC 1 cut(s) 1661
NlaIV GGNNCC 3 cut(s) 316, 567, 604
NmuCI GTSAC 2 cut(s) 1750, 1785
NsiI ATGCAT 2 cut(s) 409, 793
NspI RCATGY 2 cut(s) 776, 849
NspV TTCGAA 1 cut(s) 358
OliI CACNNNNGTG 1 cut(s) 71
PaeI GCATGC 1 cut(s) 849
PagI TCATGA 1 cut(s) 1086
PciI ACATGT 1 cut(s) 772
PctI GAATGC 2 cut(s) 1044, 2126
PdmI GAANNNNTTC 1 cut(s) 1813
PfeI GAWTC 4 cut(s) 196, 220, 455, 1441
PfoI TCCNGGA 2 cut(s) 199, 254
PinAI ACCGGT 1 cut(s) 230
PkrI GCNGC 3 cut(s) 565, 997, 1264
PleI GAGTC 4 cut(s) 387, 549, 1619, 1990
PluTI GGCGCC 3 cut(s) 318, 569, 606
PpsI GAGTC 4 cut(s) 387, 549, 1619, 1990
PscI ACATGT 1 cut(s) 772
PsiI TTATAA 3 cut(s) 42, 1323, 1865
Psp124BI GAGCTC 1 cut(s) 2104
Psp6I CCWGG 5 cut(s) 138, 199, 254, 274, 655
PspGI CCWGG 5 cut(s) 138, 199, 254, 274, 655
PspN4I GGNNCC 3 cut(s) 316, 567, 604
PspPI GGNCC 4 cut(s) 63, 136, 1327, 1900
PstNI CAGNNNCTG 1 cut(s) 2210
PsuI RGATCY 1 cut(s) 1651
PvuII CAGCTG 1 cut(s) 998
RsaI GTAC 4 cut(s) 97, 394, 1174, 1977
RsaNI GTAC 4 cut(s) 96, 393, 1173, 1976
RseI CAYNNNNRTG 3 cut(s) 71, 818, 1088
SacI GAGCTC 1 cut(s) 2104
SaqAI TTAA 5 cut(s) 53, 426, 941, 1401, 1490
SatI GCNGC 3 cut(s) 564, 996, 1263
Sau3AI GATC 3 cut(s) 583, 1537, 1651
Sau96I GGNCC 4 cut(s) 63, 136, 1327, 1900
ScaI AGTACT 2 cut(s) 97, 1174
SchI GAGTC 4 cut(s) 388, 550, 1619, 1991
ScrFI CCNGG 5 cut(s) 140, 201, 256, 276, 657
SduI GDGCHC 1 cut(s) 2104
SfaNI GCATC 5 cut(s) 778, 800, 1669, 2000, 2117
SfcI CTRYAG 1 cut(s) 1742
SfoI GGCGCC 3 cut(s) 316, 567, 604
SfuI TTCGAA 1 cut(s) 358
SinI GGWCC 3 cut(s) 63, 136, 1900
SmiMI CAYNNNNRTG 3 cut(s) 71, 818, 1088
SmlI CTYRAG 1 cut(s) 1627
SmoI CTYRAG 1 cut(s) 1627
SphI GCATGC 1 cut(s) 849
SsiI CCGC 1 cut(s) 563
SspDI GGCGCC 3 cut(s) 314, 565, 602
SspI AATATT 3 cut(s) 945, 1669, 1725
SspMI CTAG 6 cut(s) 9, 113, 1295, 1662, 1719, 1958
SstI GAGCTC 1 cut(s) 2104
StyD4I CCNGG 5 cut(s) 138, 199, 254, 274, 655
StyI CCWWGG 2 cut(s) 374, 1557
TaaI ACNGT 2 cut(s) 1952, 2206
TaqI TCGA 5 cut(s) 358, 544, 976, 1573, 2224
TatI WGTACW 3 cut(s) 95, 392, 1172
TauI GCSGC 1 cut(s) 566
TfiI GAWTC 4 cut(s) 196, 220, 455, 1441
Tru1I TTAA 5 cut(s) 53, 426, 941, 1401, 1490
Tru9I TTAA 5 cut(s) 53, 426, 941, 1401, 1490
TscAI CASTG 3 cut(s) 84, 616, 1594
TseFI GTSAC 2 cut(s) 1750, 1785
TseI GCWGC 2 cut(s) 995, 1262
Tsp45I GTSAC 2 cut(s) 1750, 1785
TspGWI ACGGA 2 cut(s) 200, 576
TspRI CASTG 3 cut(s) 84, 616, 1594
VpaK11BI GGWCC 3 cut(s) 63, 136, 1900
XapI RAATTY 8 cut(s) 108, 155, 477, 572, 754, 1397, 1698, 1811
XbaI TCTAGA 1 cut(s) 112
XceI RCATGY 2 cut(s) 776, 849
XmiI GTMKAC 2 cut(s) 883, 1219
XmnI GAANNNNTTC 1 cut(s) 1813
XspI CTAG 6 cut(s) 9, 113, 1295, 1662, 1719, 1958
ZrmI AGTACT 2 cut(s) 97, 1174
Zsp2I ATGCAT 2 cut(s) 409, 793
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.