Rw5G014000

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000112
Physical Location & Seq
Reverse (-)
16635790 .. 16638345
2556 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G014000.1

Sequence Viewer

Length: 1821 bp
ATGTACAAGGGTTTGAGTAAGTATTGGCGAAGTCCAGGGCCAACGCCTAGTGCGGTCACTAATCAAGAGGAAACTTATTATTCCCTGGATGACACCAATGCAATTACAAGAATAACGCTGTCTGATTCTGTGGAACGGCGCCTTACATGGGATGACGGTGGCCTTCAATGGAAGGAAGAATTTTCTGCACCGAAGTCCCGGTGTGACTGGTATGGACAGTGTGGTGCCAACAGCAAATGTTTCCCTGACAATGTTGGGCTGTTTGAGTGCGATTGTTTACCAGGGTATGAGCCTAGTTCTATAAATGATTGGAATCAGAAAAATGGGTCGGGTGGATGTGTGAGTAAGCGAGTTGGTGTATCAAAGTGCGGGAATAGAGATGGTTTTGTTAATGTGGCAAGAGTTAGTTGTCCAGACGCATCGATAGCATCACGGTTGAGAACAGGTATGAGTACCAAAGAGTGTGAGCAGGACTGCTTAAGAAATTGTTCTTGCACCGCATATATGAGTAATATGACCATTGACAATGAAGGACTTGCGGATTGCTTGACATGGTACGATGACTTGATGGACATTTTAGAGTTCACAGAGTTTGGACACGATTTCTACGTTCGTGTGGATCACATCGAATTAGCTGAGAATGCTAGAAAATCAAAAGGTTTCTTGGAAAGAAGGGGTATGCTGGCTATTCCAATACTCTGTGCTCTGCTAGCATTTGTACTAATCATTATGTGCGCTTGCTCGTGGCACAAGAAAAAGAGGAATACAACGGAATTTGTGGAGGCGGATGAACTGGAGGAAACCAAGAGACACCCCGAATTGCAATTTTTTGATTTGAACACACTAATGTCAGCCACAGATAACTTCTCTCATGTCAATGAACTTGGCCAAGGTGGTTTTGGCTCTGTTTATAAGGGTCAGTTACCAAATGAGCAAAAGGTAGCTGTAAAAAGGTTGTCTAAAACATCAGGACAAGGGACTGAAGAATTCAAAAATGAAGTTGCACTTATAGCAGGACTTCAACACCGCAATCTTGTAAAGCTCTTAGGCTGCTGTATAAAGGGGGAAGAACGGATGTTAGTCCTGGAATACATGCCGAACAAAAGCTTGGACTCATTTCTCTTTGATCACAGAAGGCGGTCCTTCTTGGATTGGGAAAAGCGTTTTGAAATTATCAACGGGATTGCTCGTGGAATTCTATATCTTCACCAAGACTCAAGATTGAGGATCATCCATAGAGATCTAAAAACCAGCAATGTTCTTCTAGATTCTGAAATGAACCCAAAAATTTCCGACTTTGGCATGGCAAGAATATTTGATGGTGACCAAGTTCAGAATAAAACGAACCGAGTTGTTGGAACATATGGCTACATGTCACCAGAGTATGCAGTATTTGGCAGATTTTCTACTAAATCAGATGTGTTTAGTTTTGGGATCATATTGTTAGAGGTTGTATCTGGCAAAAGAAACAATGGTTTTTCACAAGAGGATCCCTCCATGAACTTGATAAGACATGTTTGGGAACTCTGGAGAGAAGACAGAGCCTTAGAAATTGTGGATTCGACTCTGGAGTCATATAATTCTGATGAGGTCATGAGATGCATTCAAGTTGCACTCTTGTGCGTGCAAGAAGAATCAGAGGACCGGCCAGCCATGTCTGCCATTGTTTTCATGTTGAGTGGTGAAACACCTCCTCCAATATCTCCCAACCAACCTGCATATGTCTTCAGAAGAAACTCCAACACTGATGAGGAACCATCAGTACCAAAACGATTATATTCTATAAACGACTTGACAATGACTGCAATGGAAGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

606

Amino Acids

68.84

Weight (kDa)

5.36

Isoelectric Point (pI)

54.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S_locus_glycop PF00954 21 - 99 1.4e-12 S-locus glycoprotein domain
PAN_2 PF08276 123 - 192 4.6e-15 PAN-like domain
Pkinase PF00069 290 - 555 2.6e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 292 - 557 1.6e-51 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 912
AasI GACNNNNNNGTC 1 cut(s) 1570
Acc36I ACCTGC 1 cut(s) 1723
AccB1I GGYRCC 2 cut(s) 138, 224
AciI CCGC 7 cut(s) 53, 369, 498, 539, 785, 1027, 1138
AclWI GGATC 5 cut(s) 627, 1235, 1442, 1484, 1497
AcoI YGGCCR 2 cut(s) 886, 1646
AcsI RAATTY 5 cut(s) 179, 773, 986, 1194, 1287
AcuI CTGAAG 2 cut(s) 1002, 1711
AcyI GRCGYC 1 cut(s) 139
AfaI GTAC 5 cut(s) 5, 454, 557, 720, 1764
AfiI CCNNNNNNNGG 1 cut(s) 148
AflII CTTAAG 1 cut(s) 478
AflIII ACRYGT 2 cut(s) 1371, 1513
AgsI TTSAA 6 cut(s) 167, 838, 991, 1022, 1169, 1607
AjnI CCWGG 4 cut(s) 34, 84, 280, 1083
AjuI GAANNNNNNNTTGG 2 cut(s) 1091, 1123
AleI CACNNNNGTG 1 cut(s) 1618
AluBI AGCT 5 cut(s) 635, 944, 1042, 1107, 1814
AluI AGCT 5 cut(s) 635, 944, 1042, 1107, 1814
Alw21I GWGCWC 1 cut(s) 706
Alw26I GTCTC 1 cut(s) 802
AlwI GGATC 5 cut(s) 627, 1235, 1442, 1484, 1497
AoxI GGCC 4 cut(s) 38, 160, 886, 1646
ApeKI GCWGC 1 cut(s) 1050
ApoI RAATTY 5 cut(s) 179, 773, 986, 1194, 1287
Asp700I GAANNNNTTC 1 cut(s) 487
AspLEI GCGC 2 cut(s) 141, 737
AspS9I GGNCC 3 cut(s) 38, 1140, 1642
AsuC2I CCSGG 1 cut(s) 199
AsuHPI GGTGA 4 cut(s) 1199, 1334, 1368, 1694
AsuNHI GCTAGC 1 cut(s) 709
AvaII GGWCC 2 cut(s) 1140, 1642
BalI TGGCCA 1 cut(s) 888
BamHI GGATCC 1 cut(s) 1489
BanI GGYRCC 2 cut(s) 138, 224
BauI CACGAG 2 cut(s) 742, 1188
BbsI GAAGAC 2 cut(s) 1542, 1717
Bbv12I GWGCWC 1 cut(s) 706
BbvI GCAGC 1 cut(s) 1037
BccI CCATC 4 cut(s) 374, 562, 1313, 1765
BceAI ACGGC 1 cut(s) 152
BciT130I CCWGG 4 cut(s) 36, 86, 282, 1085
BclI TGATCA 1 cut(s) 1126
BcnI CCSGG 1 cut(s) 199
BcoDI GTCTC 1 cut(s) 802
BfaI CTAG 5 cut(s) 48, 294, 645, 710, 1265
BfoI RGCGCY 1 cut(s) 142
BfrI CTTAAG 1 cut(s) 478
BfuAI ACCTGC 1 cut(s) 1723
BglII AGATCT 1 cut(s) 1240
BisI GCNGC 1 cut(s) 1051
BlsI GCNGC 1 cut(s) 1052
Bme1390I CCNGG 5 cut(s) 36, 86, 199, 282, 1085
Bme18I GGWCC 2 cut(s) 1140, 1642
BmgT120I GGNCC 3 cut(s) 38, 1140, 1642
BmiI GGNNCC 4 cut(s) 140, 226, 1491, 1755
BmrFI CCNGG 5 cut(s) 36, 86, 199, 282, 1085
BmsI GCATC 3 cut(s) 428, 437, 1589
BmtI GCTAGC 1 cut(s) 713
BpiI GAAGAC 2 cut(s) 1542, 1717
BplI GAGNNNNNCTC 2 cut(s) 1478, 1510
BpmI CTGGAG 3 cut(s) 815, 1549, 1589
BpuEI CTTGAG 1 cut(s) 1201
BpuMI CCSGG 1 cut(s) 199
Bsa29I ATCGAT 1 cut(s) 422
BsaBI GATNNNNATC 1 cut(s) 312
BsaHI GRCGYC 1 cut(s) 139
BsaJI CCNNGG 4 cut(s) 35, 84, 281, 889
BsaXI ACNNNNNCTCC 2 cut(s) 1678, 1708
Bsc4I CCNNNNNNNGG 1 cut(s) 148
Bse118I RCCGGY 1 cut(s) 1644
Bse1I ACTGG 2 cut(s) 212, 798
Bse3DI GCAATG 2 cut(s) 1261, 1812
Bse8I GATNNNNATC 1 cut(s) 312
BseBI CCWGG 4 cut(s) 36, 86, 282, 1085
BseCI ATCGAT 1 cut(s) 422
BseDI CCNNGG 4 cut(s) 35, 84, 281, 889
BseGI GGATG 6 cut(s) 94, 157, 341, 793, 1080, 1230
BseJI GATNNNNATC 1 cut(s) 312
BseLI CCNNNNNNNGG 1 cut(s) 148
BseMI GCAATG 2 cut(s) 1261, 1812
BseMII CTCAG 1 cut(s) 627
BseNI ACTGG 2 cut(s) 212, 798
BseRI GAGGAG 1 cut(s) 1683
BseXI GCAGC 1 cut(s) 1037
BsgI GTGCAG 1 cut(s) 171
BshFI GGCC 4 cut(s) 40, 162, 888, 1648
BshNI GGYRCC 2 cut(s) 138, 224
BshVI ATCGAT 1 cut(s) 422
BsiHKAI GWGCWC 1 cut(s) 706
BsiSI CCGG 2 cut(s) 199, 1645
BslFI GGGAC 2 cut(s) 181, 991
BslI CCNNNNNNNGG 1 cut(s) 148
BsmAI GTCTC 1 cut(s) 802
BsmFI GGGAC 2 cut(s) 181, 991
BsmI GAATGC 2 cut(s) 646, 1602
BsnI GGCC 4 cut(s) 40, 162, 888, 1648
Bsp1286I GDGCHC 1 cut(s) 706
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 6 cut(s) 619, 1126, 1227, 1240, 1434, 1489
BspACI CCGC 7 cut(s) 53, 369, 498, 539, 785, 1027, 1138
BspANI GGCC 4 cut(s) 40, 162, 888, 1648
BspCNI CTCAG 1 cut(s) 628
BspDI ATCGAT 1 cut(s) 422
BspHI TCATGA 1 cut(s) 1593
BspLI GGNNCC 4 cut(s) 140, 226, 1491, 1755
BspMI ACCTGC 1 cut(s) 1723
BspOI GCTAGC 1 cut(s) 713
BspPI GGATC 5 cut(s) 627, 1235, 1442, 1484, 1497
BspT107I GGYRCC 2 cut(s) 138, 224
BspTI CTTAAG 1 cut(s) 478
BsrDI GCAATG 2 cut(s) 1261, 1812
BsrFI RCCGGY 1 cut(s) 1644
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 2 cut(s) 212, 798
BssAI RCCGGY 1 cut(s) 1644
BssECI CCNNGG 4 cut(s) 35, 84, 281, 889
BssMI GATC 6 cut(s) 619, 1126, 1227, 1240, 1434, 1489
BssNI GRCGYC 1 cut(s) 139
BssSI CACGAG 2 cut(s) 742, 1188
BssT1I CCWWGG 1 cut(s) 889
Bst2BI CACGAG 2 cut(s) 742, 1188
Bst2UI CCWGG 4 cut(s) 36, 86, 282, 1085
Bst4CI ACNGT 3 cut(s) 158, 219, 435
BstACI GRCGYC 1 cut(s) 139
BstAFI CTTAAG 1 cut(s) 478
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 5 cut(s) 684, 711, 739, 1625, 1650
BstDEI CTNAG 3 cut(s) 636, 1045, 1546
BstEII GGTNACC 1 cut(s) 1322
BstF5I GGATG 6 cut(s) 94, 157, 341, 793, 1080, 1230
BstH2I RGCGCY 1 cut(s) 142
BstHHI GCGC 2 cut(s) 141, 737
BstKTI GATC 6 cut(s) 622, 1129, 1230, 1243, 1437, 1492
BstMAI GTCTC 1 cut(s) 802
BstMBI GATC 6 cut(s) 619, 1126, 1227, 1240, 1434, 1489
BstMWI GCNNNNNNNGC 6 cut(s) 425, 641, 710, 1010, 1658, 1811
BstNI CCWGG 4 cut(s) 36, 86, 282, 1085
BstNSI RCATGY 3 cut(s) 1096, 1375, 1517
BstPI GGTNACC 1 cut(s) 1322
BstSCI CCNGG 5 cut(s) 34, 84, 197, 280, 1083
BstV1I GCAGC 1 cut(s) 1037
BstV2I GAAGAC 2 cut(s) 1542, 1717
BstX2I RGATCY 2 cut(s) 1240, 1489
BstYI RGATCY 2 cut(s) 1240, 1489
Bsu15I ATCGAT 1 cut(s) 422
BsuRI GGCC 4 cut(s) 40, 162, 888, 1648
BsuTUI ATCGAT 1 cut(s) 422
BtsCI GGATG 6 cut(s) 94, 157, 341, 793, 1080, 1230
BtsIMutI CAGTG 2 cut(s) 224, 1743
BveI ACCTGC 1 cut(s) 1723
Cac8I GCNNGC 5 cut(s) 684, 711, 739, 1625, 1650
CciI TCATGA 1 cut(s) 1593
CfoI GCGC 2 cut(s) 141, 737
Cfr10I RCCGGY 1 cut(s) 1644
Cfr13I GGNCC 3 cut(s) 38, 1140, 1642
ClaI ATCGAT 1 cut(s) 422
CseI GACGC 1 cut(s) 425
Csp6I GTAC 5 cut(s) 4, 453, 556, 719, 1763
CviQI GTAC 5 cut(s) 4, 453, 556, 719, 1763
DdeI CTNAG 3 cut(s) 636, 1045, 1546
DinI GGCGCC 1 cut(s) 140
DpnI GATC 6 cut(s) 621, 1128, 1229, 1242, 1436, 1491
DpnII GATC 6 cut(s) 619, 1126, 1227, 1240, 1434, 1489
DrdI GACNNNNNNGTC 1 cut(s) 1570
DseDI GACNNNNNNGTC 1 cut(s) 1570
EaeI YGGCCR 2 cut(s) 886, 1646
EciI GGCGGA 1 cut(s) 800
Eco130I CCWWGG 1 cut(s) 889
Eco47I GGWCC 2 cut(s) 1140, 1642
Eco57I CTGAAG 2 cut(s) 1002, 1711
Eco91I GGTNACC 1 cut(s) 1322
EcoO65I GGTNACC 1 cut(s) 1322
EcoRI GAATTC 2 cut(s) 986, 1194
EcoRII CCWGG 4 cut(s) 34, 84, 280, 1083
EcoT14I CCWWGG 1 cut(s) 889
EcoT22I ATGCAT 1 cut(s) 1604
EgeI GGCGCC 1 cut(s) 140
EheI GGCGCC 1 cut(s) 140
ErhI CCWWGG 1 cut(s) 889
FalI AAGNNNNNCTT 2 cut(s) 990, 1022
FaqI GGGAC 2 cut(s) 181, 991
FauI CCCGC 1 cut(s) 362
FauNDI CATATG 2 cut(s) 1363, 1720
FbaI TGATCA 1 cut(s) 1126
Fnu4HI GCNGC 1 cut(s) 1051
FokI GGATG 6 cut(s) 101, 164, 348, 800, 1087, 1217
Fsp4HI GCNGC 1 cut(s) 1051
FspBI CTAG 5 cut(s) 48, 294, 645, 710, 1265
GlaI GCGC 2 cut(s) 140, 736
GluI GCNGC 1 cut(s) 1051
GsuI CTGGAG 3 cut(s) 815, 1549, 1589
HaeII RGCGCY 1 cut(s) 142
HaeIII GGCC 4 cut(s) 40, 162, 888, 1648
HapII CCGG 2 cut(s) 199, 1645
HgaI GACGC 1 cut(s) 425
HhaI GCGC 2 cut(s) 141, 737
Hin1I GRCGYC 1 cut(s) 139
Hin6I GCGC 2 cut(s) 139, 735
HinP1I GCGC 2 cut(s) 139, 735
HindIII AAGCTT 1 cut(s) 1105
HinfI GANTC 9 cut(s) 125, 313, 1112, 1214, 1268, 1559, 1564, 1571, 1634
HpaII CCGG 2 cut(s) 199, 1645
HphI GGTGA 4 cut(s) 1199, 1334, 1368, 1694
Hpy166II GTNNAC 2 cut(s) 278, 585
Hpy188I TCNGA 9 cut(s) 124, 318, 1273, 1294, 1335, 1417, 1585, 1639, 1730
Hpy188III TCNNGA 8 cut(s) 65, 413, 969, 1218, 1265, 1528, 1568, 1594
Hpy8I GTNNAC 2 cut(s) 278, 585
HpyAV CCTTC 6 cut(s) 166, 173, 524, 666, 1128, 1153
HpyCH4III ACNGT 3 cut(s) 158, 219, 435
HpyCH4IV ACGT 1 cut(s) 609
HpyF10VI GCNNNNNNNGC 6 cut(s) 425, 641, 710, 1010, 1658, 1811
HpyF3I CTNAG 3 cut(s) 636, 1045, 1546
HpySE526I ACGT 1 cut(s) 609
Hsp92I GRCGYC 1 cut(s) 139
HspAI GCGC 2 cut(s) 139, 735
KasI GGCGCC 1 cut(s) 138
Ksp22I TGATCA 1 cut(s) 1126
Kzo9I GATC 6 cut(s) 619, 1126, 1227, 1240, 1434, 1489
Lsp1109I GCAGC 1 cut(s) 1037
LweI GCATC 3 cut(s) 428, 437, 1589
MaeI CTAG 5 cut(s) 48, 294, 645, 710, 1265
MaeII ACGT 1 cut(s) 609
MaeIII GTNAC 5 cut(s) 55, 203, 921, 1322, 1374
MalI GATC 6 cut(s) 621, 1128, 1229, 1242, 1436, 1491
MboI GATC 6 cut(s) 619, 1126, 1227, 1240, 1434, 1489
MboII GAAGA 9 cut(s) 188, 995, 1079, 1196, 1253, 1547, 1643, 1717, 1743
MflI RGATCY 2 cut(s) 1240, 1489
MhlI GDGCHC 1 cut(s) 706
MlsI TGGCCA 1 cut(s) 888
MluNI TGGCCA 1 cut(s) 888
Mly113I GGCGCC 1 cut(s) 139
MlyI GAGTC 4 cut(s) 1106, 1208, 1558, 1580
MmeI TCCRAC 3 cut(s) 1317, 1336, 1764
Mox20I TGGCCA 1 cut(s) 888
Mph1103I ATGCAT 1 cut(s) 1604
MroXI GAANNNNTTC 1 cut(s) 487
MscI TGGCCA 1 cut(s) 888
MseI TTAA 2 cut(s) 390, 479
MslI CAYNNNNRTG 3 cut(s) 845, 876, 1618
Msp20I TGGCCA 1 cut(s) 888
MspCI CTTAAG 1 cut(s) 478
MspI CCGG 2 cut(s) 199, 1645
MspR9I CCNGG 5 cut(s) 36, 86, 199, 282, 1085
Mva1269I GAATGC 2 cut(s) 646, 1602
MvaI CCWGG 4 cut(s) 36, 86, 282, 1085
MwoI GCNNNNNNNGC 6 cut(s) 425, 641, 710, 1010, 1658, 1811
NarI GGCGCC 1 cut(s) 139
NciI CCSGG 1 cut(s) 199
NdeI CATATG 2 cut(s) 1363, 1720
NdeII GATC 6 cut(s) 619, 1126, 1227, 1240, 1434, 1489
NheI GCTAGC 1 cut(s) 709
NlaIV GGNNCC 4 cut(s) 140, 226, 1491, 1755
NmuCI GTSAC 4 cut(s) 55, 203, 1322, 1374
NsiI ATGCAT 1 cut(s) 1604
NspI RCATGY 3 cut(s) 1096, 1375, 1517
OliI CACNNNNGTG 1 cut(s) 1618
PagI TCATGA 1 cut(s) 1593
PciI ACATGT 2 cut(s) 1371, 1513
PcsI WCGNNNNNNNCGW 1 cut(s) 606
PctI GAATGC 2 cut(s) 646, 1602
PdmI GAANNNNTTC 1 cut(s) 487
PfeI GAWTC 5 cut(s) 125, 313, 1268, 1559, 1634
PfoI TCCNGGA 1 cut(s) 1083
PkrI GCNGC 1 cut(s) 1052
PleI GAGTC 4 cut(s) 1106, 1208, 1558, 1579
PluTI GGCGCC 1 cut(s) 142
PpsI GAGTC 4 cut(s) 1106, 1208, 1558, 1579
PscI ACATGT 2 cut(s) 1371, 1513
PsiI TTATAA 1 cut(s) 912
Psp6I CCWGG 4 cut(s) 34, 84, 280, 1083
PspEI GGTNACC 1 cut(s) 1322
PspGI CCWGG 4 cut(s) 34, 84, 280, 1083
PspN4I GGNNCC 4 cut(s) 140, 226, 1491, 1755
PspPI GGNCC 3 cut(s) 38, 1140, 1642
PsrI GAACNNNNNNTAC 2 cut(s) 1746, 1778
PsuI RGATCY 2 cut(s) 1240, 1489
RsaI GTAC 5 cut(s) 5, 454, 557, 720, 1764
RsaNI GTAC 5 cut(s) 4, 453, 556, 719, 1763
RseI CAYNNNNRTG 3 cut(s) 845, 876, 1618
SaqAI TTAA 2 cut(s) 390, 479
SatI GCNGC 1 cut(s) 1051
Sau3AI GATC 6 cut(s) 619, 1126, 1227, 1240, 1434, 1489
Sau96I GGNCC 3 cut(s) 38, 1140, 1642
SchI GAGTC 4 cut(s) 1106, 1208, 1558, 1580
ScrFI CCNGG 5 cut(s) 36, 86, 199, 282, 1085
SduI GDGCHC 1 cut(s) 706
SfaNI GCATC 3 cut(s) 428, 437, 1589
SfoI GGCGCC 1 cut(s) 140
SinI GGWCC 2 cut(s) 1140, 1642
SmiMI CAYNNNNRTG 3 cut(s) 845, 876, 1618
SmlI CTYRAG 2 cut(s) 478, 1216
SmoI CTYRAG 2 cut(s) 478, 1216
SsiI CCGC 7 cut(s) 53, 369, 498, 539, 785, 1027, 1138
SspDI GGCGCC 1 cut(s) 138
SspI AATATT 1 cut(s) 1314
SspMI CTAG 5 cut(s) 48, 294, 645, 710, 1265
StyD4I CCNGG 5 cut(s) 34, 84, 197, 280, 1083
StyI CCWWGG 1 cut(s) 889
TaaI ACNGT 3 cut(s) 158, 219, 435
TaiI ACGT 1 cut(s) 612
TaqI TCGA 4 cut(s) 422, 627, 1562, 1816
TatI WGTACW 2 cut(s) 3, 718
TfiI GAWTC 5 cut(s) 125, 313, 1268, 1559, 1634
Tru1I TTAA 2 cut(s) 390, 479
Tru9I TTAA 2 cut(s) 390, 479
TscAI CASTG 2 cut(s) 224, 1750
TseFI GTSAC 4 cut(s) 55, 203, 1322, 1374
TseI GCWGC 1 cut(s) 1050
Tsp45I GTSAC 4 cut(s) 55, 203, 1322, 1374
TspDTI ATGAA 7 cut(s) 543, 804, 894, 1011, 1292, 1514, 1660
TspGWI ACGGA 2 cut(s) 785, 1087
TspRI CASTG 2 cut(s) 224, 1750
Vha464I CTTAAG 1 cut(s) 478
VpaK11BI GGWCC 2 cut(s) 1140, 1642
XapI RAATTY 5 cut(s) 179, 773, 986, 1194, 1287
XbaI TCTAGA 1 cut(s) 1264
XceI RCATGY 3 cut(s) 1096, 1375, 1517
XcmI CCANNNNNNNNNTGG 1 cut(s) 896
XmnI GAANNNNTTC 1 cut(s) 487
XspI CTAG 5 cut(s) 48, 294, 645, 710, 1265
Zsp2I ATGCAT 1 cut(s) 1604
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.