RchiOBHm_Chr4g0413551

serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000112
Physical Location & Seq
Reverse (-)
37809857 .. 37812987
3131 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ38405

Sequence Viewer

Length: 2421 bp
ATGTTGTTCAAAACTCTGCTTCTCTTCTTCCTCTTCAGATTTTGCGATTCCAGGGACACCATAACATGGAACGAGGAACAACTAAAGGATGATGGAGGAGTTTTAGTGTCCAAACAAACCAAATTCGAATTGGGTTTCTTCAGCCCCGGAAATTCTAGCTACCGGTATGTTGGAATTTGGTACTCTTTGGCTGAAGTATCTGAAAAAACAGTGGTGTGGGTCGCAAACAGGAACAATCCCATCAATGATACGTCTGGTGTGCTCACAATAAACAGGTATGGAGATCTAGTCCTTTATGCTCATAACATGGAGAGCACTCCTATTTGGTCTACTAATGTGTCGCGGTCGGTCCAAAATGTTAACTCAAGCACTTTATCTGCACAGCTTTTAGATACAGGAAATTTAGTTGTATTCCAGGCTGATCTCTCTATATGGCAAAGTTTTGATTATCCCACAGATACTCTAATTCCAGGTATGAAAGTTGGGGTGGATTGGAAAACTGGTCTAGAATGGGTTTTAACATCTTGGAAGTCACAAGAGGACCCTGGAACTGGGGACTGTACCTTTAGGCTAAGTTCAGATCAGATTGCATCTCCCCAATTTGTTCTGTATAAAGGTTTGAGTAGGTATTGGCGAGGTGATCCGGCGCCAACGCCTAGTTTGGTCACTACTCAAGAGGAAACTTATTATTTCATGAATGACCTCGCAATTACAAGAACAACAGTAACTGATTCTGCCTTAGAGCGCCGTACATGGGATGATGGTGGCCTTCAATGGAAGGAAGACTTCTCTGCACCGAAGTCCCGGTGTGATTGGTATGGACAGTGTGGTGCCAACAGCAAATGTAGCCCTGACAATGTTAATATGTTTTGGTGTGATTGTTTACCAGGGTATGTGCCTAATTCTTTAAGTGATTGGAATCAGAAAAATTGGTCAGGTGGATGTGTGAGTAATCGACTTGGTTTGTTGAAGTGTGGCGATGGAGATGGGTTTGTGAAGGTGGCAAAAGTTAAATATCCAGACACATCGATAGCAGAATCGTTAAAGTCAGGAATGAGTGACAAAGAATGTGAGCAGGAGTGCCTAAGAAATTGTTCTTGCACTGCATATTTGAGCTCTGAAAATGAAGGGACTGTTGATTGCTTGACATGGTATGATGACTTGATGGACATTTTAGTGTACACAGAGCTTGGACAAGATCTCTACGTTCGTGTGAATGCAACTGTGTTAGCGGAAAATGGCGGAAGATCAGAAGGTTTCTTGGAAAGGAGAGGTATGCTGGCTATTCCAATCCTTTCTGCTGTGCTAGCAATGGTACTAATCATTGTGCTTGCATGTTGGTGGCGTAAGAAGAACAGGAACACAAAAGGCATAGACTTTGTGGAGGCAGAGGAGCTTGTGGAAACCCAGAGACACCCTGAATTGCAATTTTTCGATCTTGACACAATAATAGCAGCCACAGACCACTTCTCTCGTGTCAATGAACTTGGCCATGGTGGTTTTGGCTCTGTTTATAAGGGTCAGCTACCAAATGAACAGAAAGTTGCTGTGAAAAGATTGTCCAAAACTTCAGGACAAGGGATCGAAGAATTCAAAAATGAAGTTTTACTTATAGCAAGACTTCAACACAGGAATCTAGTGAAACTTTTAGGCTGTTGTATAAAGGGAGAAGAAAGGATGTTAGTGCTAGAATACATGCCTAACAATAGCTTGAACTCCTTTCTTTTTGATGACACAAGACGGTCCTTCTTGGATTGGGAAAAGCGCTTTGAAATTATCAACGGGATTGCTCGTGGGATTTTGTATCTTCACCAAGACTCAAGATTGAGGATCATCCATAGAGATCTGAAAACTAGTAATATTCTTCTAGATGCTGAGATGAACCCAAGAATTTCTGATTTTGGCATGGCTAGAATATTCCACGGAGACCAATTGCAAGATAAGACAAGGCGAATTGTTGGAACATATGGCTACATGTCACCAGAATACGCAGTATTTGGGAGATTTTCAACGAAATCTGATGTATTTAGTTTTGGGATCATAATGTTGGAGATTGTAAGTGGCCGGAAAAACAATGGCTCTGATCTAGAGGATCCTTCCATGAACTTGATAGGACGTGTTTGGGAGCTCTGGGGAGAAGGCAGAGCCTTAGATATTGTTGATTCGACACTGAAGTCATATAAGCCTAATGAAGTCATGAGATGCATACAAGTTGCGCTCTTGTGTGTACAAGAAGATTCAAAGGACCGACCTGCCATGTCAGCCATTGTTTTCATGTTGAGTGGTGAAGCATCTCCTCCATTGCCTAAGCATCCGGCATTTGTTTACAGAAGAGATTCCGACGCTGATGTTGATCCGTTACATGCAAACGGAACTGATTCTATAAATGACTTGACAATAACTACAATGAAAGCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B_lectin DUF3403 PAN_2 Pkinase Pkinase_Tyr S_locus_glycop

Protein Analysis

806

Amino Acids

91.09

Weight (kDa)

5.22

Isoelectric Point (pI)

37.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 70 - 177 3.6e-33 D-mannose binding lectin
S_locus_glycop PF00954 227 - 301 1e-12 S-locus glycoprotein domain
PAN_2 PF08276 325 - 391 8.3e-18 PAN-like domain
Pkinase PF00069 491 - 756 2.2e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 493 - 758 3.7e-50 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000112)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g03320 FvH4_3g03322 FvH4_3g03323 FvH4_3g03340 FvH4_3g03350 FvH4_3g03351 FvH4_3g03370 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03390 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g03410 FvH4_3g06140 FvH4_6g00257 FvH4_6g00257 FvH4_6g00270 FvH4_6g00300 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800 FvH4_6g20800
malus_domestica MD05G1232700.v1.1 MD05G1327700.v1.1 MD05G1327800.v1.1 MD05G1327900.v1.1 MD05G1328000.v1.1 MD05G1328200.v1.1 MD05G1328300.v1.1 MD05G1328500.v1.1 MD05G1332000.v1.1 MD05G1332100.v1.1 MD10G1307600.v1.1 MD10G1307800.v1.1
prunus_persica Prupe.4G031900_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032000_v2.0.a1 Prupe.4G032100_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1 Prupe.4G032200_v2.0.a1
pyrus_communis pycom05g30180 pycom05g30190 pycom05g30200 pycom05g30210 pycom10g25920 pycom10g25930
rosa_chinensis RchiOBHm_Chr2g0130831 RchiOBHm_Chr2g0132531 RchiOBHm_Chr2g0132541 RchiOBHm_Chr3g0455681 RchiOBHm_Chr3g0476681 RchiOBHm_Chr3g0476761 RchiOBHm_Chr3g0476771 RchiOBHm_Chr3g0476791 RchiOBHm_Chr3g0476801 RchiOBHm_Chr3g0476811 RchiOBHm_Chr4g0413551 RchiOBHm_Chr4g0413561 RchiOBHm_Chr5g0002911 RchiOBHm_Chr5g0005071 RchiOBHm_Chr5g0005101 RchiOBHm_Chr5g0005111 RchiOBHm_Chr5g0005121 RchiOBHm_Chr5g0005131 RchiOBHm_Chr5g0005141 RchiOBHm_Chr5g0005161 RchiOBHm_Chr5g0005171 RchiOBHm_Chr5g0006341 RchiOBHm_Chr5g0046161 RchiOBHm_Chr5g0046171 RchiOBHm_Chr5g0046181 RchiOBHm_Chr5g0046221 RchiOBHm_Chr5g0046231 RchiOBHm_Chr7g0224061 RchiOBHm_Chr7g0224081 RchiOBHm_Chr7g0224101 RchiOBHm_Chr7g0224111 RchiOBHm_Chr7g0224121
rosa_laevigata RLG00000010193 RLG00000019146 RLG00000019148 RLG00000019149 RLG00000019150 RLG00000023750 RLG00000023751 RLG00000023753 RLG00000029486 RLG00000031068 RLG00000031069 RLG00000031284 RLG00000031285 RLG00000031286 RLG00000031289 RLG00000031290 RLG00000031291 RLG00000031292 RLG00000031293 RLG00000031294 RLG00000031295 RLG00000031296 RLG00000031297 RLG00000031363 RLG00000032618 RLG00000032619
rosa_multiflora Rmu_co8147584.1_g000001 Rmu_co8205828.1_g000001 Rmu_co8373645.1_g000001 Rmu_co8407679.1_g000001 Rmu_co8452561.1_g000001 Rmu_sc0001035.1_g000076 Rmu_sc0001884.1_g000026 Rmu_sc0001884.1_g000027 Rmu_sc0001884.1_g000035 Rmu_sc0002157.1_g000026 Rmu_sc0002399.1_g000006 Rmu_sc0002399.1_g000007 Rmu_sc0002883.1_g000010 Rmu_sc0003629.1_g000014 Rmu_sc0003884.1_g000004 Rmu_sc0007865.1_g000005 Rmu_sc0008085.1_g000013 Rmu_sc0008085.1_g000014 Rmu_sc0008085.1_g000016 Rmu_sc0008085.1_g000018 Rmu_sc0008432.1_g000004 Rmu_sc0008714.1_g000002 Rmu_sc0009296.1_g000008 Rmu_sc0009296.1_g000009 Rmu_sc0009296.1_g000011 Rmu_sc0010281.1_g000001 Rmu_sc0010281.1_g000002 Rmu_sc0010714.1_g000011 Rmu_sc0010714.1_g000016 Rmu_sc0011169.1_g000001 Rmu_sc0011169.1_g000007 Rmu_sc0013603.1_g000006 Rmu_sc0020478.1_g000003 Rmu_sc0020478.1_g000007 Rmu_sc0025106.1_g000012 Rmu_sc0032709.1_g000001 Rmu_sc0033028.1_g000001
rosa_roxburghii Rroxscaffold_1G00069570 Rroxscaffold_1G00069610 Rroxscaffold_1G00069620 Rroxscaffold_1G00070480 Rroxscaffold_1G00070490 Rroxscaffold_1G00070500 Rroxscaffold_1G00070510 Rroxscaffold_1G00070520 Rroxscaffold_1G00070530 Rroxscaffold_1G00070540 Rroxscaffold_1G00070550 Rroxscaffold_1G00070590 Rroxscaffold_1G00072950 Rroxscaffold_1G00073080 Rroxscaffold_2G00111810 Rroxscaffold_2G00111820 Rroxscaffold_2G00113530 Rroxscaffold_2G00113870 Rroxscaffold_6G00405010 Rroxscaffold_6G00405020 Rroxscaffold_6G00423590 Rroxscaffold_6G00424100
rosa_rugosa Rorug02G0293200 Rorug02G0293300 Rorug02G0293400 Rorug02G0305900 Rorug02G0305900 Rorug03G0009100 Rorug03G0157000 Rorug03G0157000 Rorug03G0304900 Rorug04G0400500 Rorug04G0415200 Rorug04G0415300 Rorug04G0415400 Rorug04G0415500 Rorug04G0421100 Rorug05G0068000 Rorug05G0068100 Rorug05G0068200 Rorug05G0068500 Rorug05G0224600 Rorug05G0224700
rosa_samantha Rh2CG331700 Rh2CG341600 Rh2CG341700 Rh2DG381200 Rh3DG071200 Rh3DG233400 Rh3DG233500 Rh3DG233600 Rh5AG043100 Rh5CG026000 Rh5CG047700 Rh5CG047800 Rh5CG047900 Rh5CG048000 Rh5CG048100 Rh5CG048300 Rh5CG056500 Rh5CG340500 Rh5CG340700 Rh5CG340800 Rh7AG365000
rosa_wichuraiana Rw0G002360 Rw0G002370 Rw0G012520 Rw0G018230 Rw0G019190 Rw2G027480 Rw2G029150 Rw3G005520 Rw3G005880 Rw3G005890 Rw3G018840 Rw3G018850 Rw3G018870 Rw3G018880 Rw3G020740 Rw5G002190 Rw5G004180 Rw5G004190 Rw5G004890 Rw5G013960 Rw5G014000 Rw5G028610 Rw7G030910 Rw7G030920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1515
AasI GACNNNNNNGTC 1 cut(s) 2173
Acc36I ACCTGC 1 cut(s) 2260
AccB1I GGYRCC 2 cut(s) 646, 830
AccB7I CCANNNNNTGG 1 cut(s) 66
AccI GTMKAC 1 cut(s) 329
AccII CGCG 1 cut(s) 343
AciI CCGC 3 cut(s) 343, 1232, 1242
AclWI GGATC 7 cut(s) 635, 1589, 1838, 2045, 2087, 2100, 2348
AcoI YGGCCR 2 cut(s) 1489, 2062
AcsI RAATTY 6 cut(s) 122, 151, 174, 400, 1589, 1890
AcuI CTGAAG 5 cut(s) 19, 124, 213, 1554, 2192
AcyI GRCGYC 1 cut(s) 647
AfaI GTAC 6 cut(s) 182, 562, 751, 1181, 1317, 2229
AfeI AGCGCT 1 cut(s) 1766
AfiI CCNNNNNNNGG 3 cut(s) 66, 551, 754
AflIII ACRYGT 2 cut(s) 1974, 2116
AgeI ACCGGT 1 cut(s) 162
AgsI TTSAA 9 cut(s) 10, 773, 970, 1594, 1625, 1714, 1772, 2010, 2241
AhlI ACTAGT 1 cut(s) 1853
AjiI CACGTC 1 cut(s) 2117
AjnI CCWGG 5 cut(s) 50, 414, 469, 544, 886
AluBI AGCT 9 cut(s) 159, 385, 1116, 1189, 1396, 1525, 1710, 2128, 2414
AluI AGCT 9 cut(s) 159, 385, 1116, 1189, 1396, 1525, 1710, 2128, 2414
Alw21I GWGCWC 4 cut(s) 264, 317, 1118, 2130
Alw26I GTCTC 2 cut(s) 1405, 1920
AlwI GGATC 7 cut(s) 635, 1589, 1838, 2045, 2087, 2100, 2348
AlwNI CAGNNNCTG 1 cut(s) 728
Aor51HI AGCGCT 1 cut(s) 1766
AoxI GGCC 3 cut(s) 766, 1489, 2062
ApeKI GCWGC 1 cut(s) 1454
ApoI RAATTY 6 cut(s) 122, 151, 174, 400, 1589, 1890
AsiGI ACCGGT 1 cut(s) 162
Asp700I GAANNNNTTC 3 cut(s) 1093, 2335, 2377
AspLEI GCGC 4 cut(s) 649, 747, 1767, 2218
AspS9I GGNCC 4 cut(s) 349, 541, 1743, 2245
AsuC2I CCSGG 2 cut(s) 147, 805
AsuHPI GGTGA 4 cut(s) 650, 1802, 1971, 2297
AsuII TTCGAA 1 cut(s) 126
AsuNHI GCTAGC 1 cut(s) 1306
AvaII GGWCC 4 cut(s) 349, 541, 1743, 2245
BalI TGGCCA 1 cut(s) 1491
BamHI GGATCC 1 cut(s) 2092
BanI GGYRCC 2 cut(s) 646, 830
BanII GRGCYC 2 cut(s) 1118, 2130
BauI CACGAG 2 cut(s) 1473, 1791
BbsI GAAGAC 1 cut(s) 789
Bbv12I GWGCWC 4 cut(s) 264, 317, 1118, 2130
BbvI GCAGC 1 cut(s) 1466
BccI CCATC 6 cut(s) 86, 248, 755, 974, 980, 1159
BceAI ACGGC 1 cut(s) 732
BciT130I CCWGG 5 cut(s) 52, 416, 471, 546, 888
BcnI CCSGG 2 cut(s) 147, 805
BcoDI GTCTC 2 cut(s) 1405, 1920
BcuI ACTAGT 1 cut(s) 1853
BfoI RGCGCY 3 cut(s) 650, 748, 1768
BfuAI ACCTGC 1 cut(s) 2260
BglII AGATCT 3 cut(s) 283, 1198, 1843
BisI GCNGC 1 cut(s) 1455
BlsI GCNGC 1 cut(s) 1456
Bme1390I CCNGG 7 cut(s) 52, 147, 416, 471, 546, 805, 888
Bme18I GGWCC 4 cut(s) 349, 541, 1743, 2245
BmgBI CACGTC 1 cut(s) 2117
BmgT120I GGNCC 4 cut(s) 349, 541, 1743, 2245
BmiI GGNNCC 4 cut(s) 543, 648, 832, 2094
BmrFI CCNGG 7 cut(s) 52, 147, 416, 471, 546, 805, 888
BmrI ACTGGG 1 cut(s) 561
BmsI GCATC 5 cut(s) 599, 1861, 2192, 2300, 2320
BmtI GCTAGC 1 cut(s) 1310
BmuI ACTGGG 1 cut(s) 561
BpiI GAAGAC 1 cut(s) 789
Bpu10I CCTNAGC 1 cut(s) 2307
Bpu14I TTCGAA 1 cut(s) 126
BpuEI CTTGAG 3 cut(s) 349, 657, 1804
BpuMI CCSGG 2 cut(s) 147, 805
Bsa29I ATCGAT 1 cut(s) 1028
BsaBI GATNNNNATC 2 cut(s) 918, 2352
BsaHI GRCGYC 1 cut(s) 647
BsaI GGTCTC 1 cut(s) 1920
BsaJI CCNNGG 6 cut(s) 51, 145, 544, 887, 1492, 1921
BsaWI WCCGGW 1 cut(s) 162
BsaXI ACNNNNNCTCC 4 cut(s) 90, 120, 273, 303
Bsc4I CCNNNNNNNGG 3 cut(s) 66, 551, 754
Bse118I RCCGGY 1 cut(s) 162
Bse1I ACTGG 2 cut(s) 505, 556
Bse3DI GCAATG 2 cut(s) 1317, 2300
Bse8I GATNNNNATC 2 cut(s) 918, 2352
BseBI CCWGG 5 cut(s) 52, 416, 471, 546, 888
BseCI ATCGAT 1 cut(s) 1028
BseDI CCNNGG 6 cut(s) 51, 145, 544, 887, 1492, 1921
BseGI GGATG 6 cut(s) 94, 763, 947, 1683, 1833, 2311
BseJI GATNNNNATC 2 cut(s) 918, 2352
BseLI CCNNNNNNNGG 3 cut(s) 66, 551, 754
BseMI GCAATG 2 cut(s) 1317, 2300
BseMII CTCAG 1 cut(s) 1866
BseNI ACTGG 2 cut(s) 505, 556
BseRI GAGGAG 3 cut(s) 111, 1406, 2286
BseXI GCAGC 1 cut(s) 1466
BsgI GTGCAG 2 cut(s) 363, 777
Bsh1236I CGCG 1 cut(s) 343
Bsh1285I CGRYCG 1 cut(s) 347
BshFI GGCC 3 cut(s) 768, 1491, 2064
BshNI GGYRCC 2 cut(s) 646, 830
BshTI ACCGGT 1 cut(s) 162
BshVI ATCGAT 1 cut(s) 1028
BsiEI CGRYCG 1 cut(s) 347
BsiHKAI GWGCWC 4 cut(s) 264, 317, 1118, 2130
BsiSI CCGG 6 cut(s) 147, 163, 644, 805, 2065, 2315
BslFI GGGAC 4 cut(s) 68, 569, 787, 1144
BslI CCNNNNNNNGG 3 cut(s) 66, 551, 754
BsmAI GTCTC 2 cut(s) 1405, 1920
BsmFI GGGAC 4 cut(s) 68, 569, 787, 1144
BsmI GAATGC 1 cut(s) 1222
BsnI GGCC 3 cut(s) 768, 1491, 2064
Bso31I GGTCTC 1 cut(s) 1920
Bsp119I TTCGAA 1 cut(s) 126
Bsp1286I GDGCHC 4 cut(s) 264, 317, 1118, 2130
Bsp1407I TGTACA 2 cut(s) 1179, 2227
Bsp19I CCATGG 1 cut(s) 1492
BspACI CCGC 3 cut(s) 343, 1232, 1242
BspANI GGCC 3 cut(s) 768, 1491, 2064
BspCNI CTCAG 1 cut(s) 1867
BspDI ATCGAT 1 cut(s) 1028
BspFNI CGCG 1 cut(s) 343
BspHI TCATGA 2 cut(s) 693, 2196
BspLI GGNNCC 4 cut(s) 543, 648, 832, 2094
BspMI ACCTGC 1 cut(s) 2260
BspOI GCTAGC 1 cut(s) 1310
BspPI GGATC 7 cut(s) 635, 1589, 1838, 2045, 2087, 2100, 2348
BspT104I TTCGAA 1 cut(s) 126
BspT107I GGYRCC 2 cut(s) 646, 830
BspTNI GGTCTC 1 cut(s) 1920
BsrDI GCAATG 2 cut(s) 1317, 2300
BsrFI RCCGGY 1 cut(s) 162
BsrGI TGTACA 2 cut(s) 1179, 2227
BsrI ACTGG 2 cut(s) 505, 556
BssAI RCCGGY 1 cut(s) 162
BssECI CCNNGG 6 cut(s) 51, 145, 544, 887, 1492, 1921
BssNI GRCGYC 1 cut(s) 647
BssSI CACGAG 2 cut(s) 1473, 1791
BssT1I CCWWGG 1 cut(s) 1492
Bst2BI CACGAG 2 cut(s) 1473, 1791
Bst2UI CCWGG 5 cut(s) 52, 416, 471, 546, 888
Bst4CI ACNGT 7 cut(s) 211, 560, 724, 825, 1135, 1225, 1743
Bst6I CTCTTC 3 cut(s) 29, 38, 2326
BstACI GRCGYC 1 cut(s) 647
BstAUI TGTACA 2 cut(s) 1179, 2227
BstBI TTCGAA 1 cut(s) 126
BstC8I GCNNGC 3 cut(s) 1281, 1308, 1332
BstDEI CTNAG 6 cut(s) 572, 739, 1085, 1875, 2149, 2307
BstDSI CCRYGG 2 cut(s) 1492, 1921
BstF5I GGATG 6 cut(s) 94, 763, 947, 1683, 1833, 2311
BstFNI CGCG 1 cut(s) 343
BstH2I RGCGCY 3 cut(s) 650, 748, 1768
BstHHI GCGC 4 cut(s) 649, 747, 1767, 2218
BstMAI GTCTC 2 cut(s) 1405, 1920
BstMCI CGRYCG 1 cut(s) 347
BstMWI GCNNNNNNNGC 3 cut(s) 846, 1307, 2261
BstNI CCWGG 5 cut(s) 52, 416, 471, 546, 888
BstNSI RCATGY 4 cut(s) 1338, 1699, 1978, 2366
BstSCI CCNGG 7 cut(s) 50, 145, 414, 469, 544, 803, 886
BstUI CGCG 1 cut(s) 343
BstV1I GCAGC 1 cut(s) 1466
BstV2I GAAGAC 1 cut(s) 789
BstX2I RGATCY 4 cut(s) 283, 1198, 1843, 2092
BstYI RGATCY 4 cut(s) 283, 1198, 1843, 2092
Bsu15I ATCGAT 1 cut(s) 1028
BsuRI GGCC 3 cut(s) 768, 1491, 2064
BsuTUI ATCGAT 1 cut(s) 1028
BtgI CCRYGG 2 cut(s) 1492, 1921
BtgZI GCGATG 1 cut(s) 993
BtrI CACGTC 1 cut(s) 2117
BtsCI GGATG 6 cut(s) 94, 763, 947, 1683, 1833, 2311
BtsI GCAGTG 1 cut(s) 1101
BtsIMutI CAGTG 4 cut(s) 216, 830, 1101, 2168
BveI ACCTGC 1 cut(s) 2260
Cac8I GCNNGC 3 cut(s) 1281, 1308, 1332
CaiI CAGNNNCTG 1 cut(s) 728
CciI TCATGA 2 cut(s) 693, 2196
CfoI GCGC 4 cut(s) 649, 747, 1767, 2218
Cfr10I RCCGGY 1 cut(s) 162
Cfr13I GGNCC 4 cut(s) 349, 541, 1743, 2245
ClaI ATCGAT 1 cut(s) 1028
CseI GACGC 1 cut(s) 2351
Csp6I GTAC 6 cut(s) 181, 561, 750, 1180, 1316, 2228
CspAI ACCGGT 1 cut(s) 162
CviQI GTAC 6 cut(s) 181, 561, 750, 1180, 1316, 2228
DdeI CTNAG 6 cut(s) 572, 739, 1085, 1875, 2149, 2307
DinI GGCGCC 1 cut(s) 648
DrdI GACNNNNNNGTC 1 cut(s) 2173
DseDI GACNNNNNNGTC 1 cut(s) 2173
EaeI YGGCCR 2 cut(s) 1489, 2062
Eam1104I CTCTTC 3 cut(s) 29, 38, 2326
EarI CTCTTC 3 cut(s) 29, 38, 2326
EciI GGCGGA 1 cut(s) 1257
Ecl136II GAGCTC 2 cut(s) 1116, 2128
Eco130I CCWWGG 1 cut(s) 1492
Eco24I GRGCYC 2 cut(s) 1118, 2130
Eco31I GGTCTC 1 cut(s) 1920
Eco47I GGWCC 4 cut(s) 349, 541, 1743, 2245
Eco47III AGCGCT 1 cut(s) 1766
Eco53kI GAGCTC 2 cut(s) 1116, 2128
Eco57I CTGAAG 5 cut(s) 19, 124, 213, 1554, 2192
EcoICRI GAGCTC 2 cut(s) 1116, 2128
EcoO109I RGGNCCY 1 cut(s) 541
EcoRI GAATTC 1 cut(s) 1589
EcoRII CCWGG 5 cut(s) 50, 414, 469, 544, 886
EcoT14I CCWWGG 1 cut(s) 1492
EcoT22I ATGCAT 1 cut(s) 2207
EcoT38I GRGCYC 2 cut(s) 1118, 2130
EgeI GGCGCC 1 cut(s) 648
EheI GGCGCC 1 cut(s) 648
ErhI CCWWGG 1 cut(s) 1492
FalI AAGNNNNNCTT 4 cut(s) 770, 802, 1593, 1625
FaqI GGGAC 4 cut(s) 68, 569, 787, 1144
FauNDI CATATG 1 cut(s) 1966
FblI GTMKAC 1 cut(s) 329
Fnu4HI GCNGC 1 cut(s) 1455
FokI GGATG 6 cut(s) 101, 770, 954, 1690, 1820, 2298
FriOI GRGCYC 2 cut(s) 1118, 2130
Fsp4HI GCNGC 1 cut(s) 1455
GlaI GCGC 4 cut(s) 648, 746, 1766, 2217
GluI GCNGC 1 cut(s) 1455
HaeII RGCGCY 3 cut(s) 650, 748, 1768
HaeIII GGCC 3 cut(s) 768, 1491, 2064
HapII CCGG 6 cut(s) 147, 163, 644, 805, 2065, 2315
HgaI GACGC 1 cut(s) 2351
HhaI GCGC 4 cut(s) 649, 747, 1767, 2218
Hin1I GRCGYC 1 cut(s) 647
Hin6I GCGC 4 cut(s) 647, 745, 1765, 2216
HinP1I GCGC 4 cut(s) 647, 745, 1765, 2216
HincII GTYRAC 1 cut(s) 361
HindII GTYRAC 1 cut(s) 361
HpaI GTTAAC 1 cut(s) 361
HpaII CCGG 6 cut(s) 147, 163, 644, 805, 2065, 2315
HphI GGTGA 4 cut(s) 650, 1802, 1971, 2297
Hpy166II GTNNAC 7 cut(s) 330, 361, 884, 1180, 1182, 2228, 2326
Hpy8I GTNNAC 7 cut(s) 330, 361, 884, 1180, 1182, 2228, 2326
Hpy99I CGWCG 1 cut(s) 2345
HpyAV CCTTC 8 cut(s) 772, 779, 991, 1121, 1247, 1756, 2106, 2132
HpyCH4III ACNGT 7 cut(s) 211, 560, 724, 825, 1135, 1225, 1743
HpyCH4IV ACGT 3 cut(s) 251, 1206, 2116
HpyF10VI GCNNNNNNNGC 3 cut(s) 846, 1307, 2261
HpyF3I CTNAG 6 cut(s) 572, 739, 1085, 1875, 2149, 2307
HpySE526I ACGT 3 cut(s) 251, 1206, 2116
Hsp92I GRCGYC 1 cut(s) 647
HspAI GCGC 4 cut(s) 647, 745, 1765, 2216
KasI GGCGCC 1 cut(s) 646
KspAI GTTAAC 1 cut(s) 361
LmnI GCTCC 2 cut(s) 1393, 2125
Lsp1109I GCAGC 1 cut(s) 1466
LweI GCATC 5 cut(s) 599, 1861, 2192, 2300, 2320
MaeII ACGT 3 cut(s) 251, 1206, 2116
MaeIII GTNAC 6 cut(s) 531, 664, 724, 1058, 1977, 2358
MfeI CAATTG 1 cut(s) 1931
MflI RGATCY 4 cut(s) 283, 1198, 1843, 2092
MhlI GDGCHC 4 cut(s) 264, 317, 1118, 2130
MlsI TGGCCA 1 cut(s) 1491
MluNI TGGCCA 1 cut(s) 1491
Mly113I GGCGCC 1 cut(s) 647
MlyI GAGTC 1 cut(s) 1811
MmeI TCCRAC 4 cut(s) 151, 1939, 2028, 2364
Mox20I TGGCCA 1 cut(s) 1491
Mph1103I ATGCAT 1 cut(s) 2207
MroXI GAANNNNTTC 3 cut(s) 1093, 2335, 2377
MscI TGGCCA 1 cut(s) 1491
MseI TTAA 6 cut(s) 360, 518, 861, 908, 1011, 1043
MslI CAYNNNNRTG 2 cut(s) 1175, 1339
Msp20I TGGCCA 1 cut(s) 1491
MspI CCGG 6 cut(s) 147, 163, 644, 805, 2065, 2315
MspR9I CCNGG 7 cut(s) 52, 147, 416, 471, 546, 805, 888
MunI CAATTG 1 cut(s) 1931
Mva1269I GAATGC 1 cut(s) 1222
MvaI CCWGG 5 cut(s) 52, 416, 471, 546, 888
MvnI CGCG 1 cut(s) 343
MwoI GCNNNNNNNGC 3 cut(s) 846, 1307, 2261
NarI GGCGCC 1 cut(s) 647
NciI CCSGG 2 cut(s) 147, 805
NcoI CCATGG 1 cut(s) 1492
NdeI CATATG 1 cut(s) 1966
NheI GCTAGC 1 cut(s) 1306
NlaIV GGNNCC 4 cut(s) 543, 648, 832, 2094
NmuCI GTSAC 4 cut(s) 531, 664, 1058, 1977
NsiI ATGCAT 1 cut(s) 2207
NspI RCATGY 4 cut(s) 1338, 1699, 1978, 2366
NspV TTCGAA 1 cut(s) 126
PagI TCATGA 2 cut(s) 693, 2196
PciI ACATGT 1 cut(s) 1974
PctI GAATGC 1 cut(s) 1222
PdmI GAANNNNTTC 3 cut(s) 1093, 2335, 2377
PfeI GAWTC 9 cut(s) 47, 731, 919, 1037, 1633, 2162, 2237, 2336, 2378
PflMI CCANNNNNTGG 1 cut(s) 66
PinAI ACCGGT 1 cut(s) 162
PkrI GCNGC 1 cut(s) 1456
PleI GAGTC 1 cut(s) 1811
PluTI GGCGCC 1 cut(s) 650
PpsI GAGTC 1 cut(s) 1811
PpuMI RGGWCCY 1 cut(s) 541
PscI ACATGT 1 cut(s) 1974
PsiI TTATAA 1 cut(s) 1515
Psp124BI GAGCTC 2 cut(s) 1118, 2130
Psp5II RGGWCCY 1 cut(s) 541
Psp6I CCWGG 5 cut(s) 50, 414, 469, 544, 886
PspGI CCWGG 5 cut(s) 50, 414, 469, 544, 886
PspN4I GGNNCC 4 cut(s) 543, 648, 832, 2094
PspPI GGNCC 4 cut(s) 349, 541, 1743, 2245
PspPPI RGGWCCY 1 cut(s) 541
PstNI CAGNNNCTG 1 cut(s) 728
PsuI RGATCY 4 cut(s) 283, 1198, 1843, 2092
RsaI GTAC 6 cut(s) 182, 562, 751, 1181, 1317, 2229
RsaNI GTAC 6 cut(s) 181, 561, 750, 1180, 1316, 2228
RseI CAYNNNNRTG 2 cut(s) 1175, 1339
SacI GAGCTC 2 cut(s) 1118, 2130
SaqAI TTAA 6 cut(s) 360, 518, 861, 908, 1011, 1043
SatI GCNGC 1 cut(s) 1455
Sau96I GGNCC 4 cut(s) 349, 541, 1743, 2245
SchI GAGTC 1 cut(s) 1811
ScrFI CCNGG 7 cut(s) 52, 147, 416, 471, 546, 805, 888
SduI GDGCHC 4 cut(s) 264, 317, 1118, 2130
SfaNI GCATC 5 cut(s) 599, 1861, 2192, 2300, 2320
SfoI GGCGCC 1 cut(s) 648
SfuI TTCGAA 1 cut(s) 126
SinI GGWCC 4 cut(s) 349, 541, 1743, 2245
SmiMI CAYNNNNRTG 2 cut(s) 1175, 1339
SmlI CTYRAG 3 cut(s) 364, 672, 1819
SmoI CTYRAG 3 cut(s) 364, 672, 1819
SpeI ACTAGT 1 cut(s) 1853
SsiI CCGC 3 cut(s) 343, 1232, 1242
SspDI GGCGCC 1 cut(s) 646
SspI AATATT 2 cut(s) 1861, 1917
SstI GAGCTC 2 cut(s) 1118, 2130
StyD4I CCNGG 7 cut(s) 50, 145, 414, 469, 544, 803, 886
StyI CCWWGG 1 cut(s) 1492
TaaI ACNGT 7 cut(s) 211, 560, 724, 825, 1135, 1225, 1743
TaiI ACGT 3 cut(s) 254, 1209, 2119
TaqI TCGA 7 cut(s) 126, 955, 1028, 1434, 1584, 2165, 2416
TaqII GACCGA 2 cut(s) 337, 2262
TatI WGTACW 2 cut(s) 1179, 2227
TfiI GAWTC 9 cut(s) 47, 731, 919, 1037, 1633, 2162, 2237, 2336, 2378
Tru1I TTAA 6 cut(s) 360, 518, 861, 908, 1011, 1043
Tru9I TTAA 6 cut(s) 360, 518, 861, 908, 1011, 1043
TscAI CASTG 4 cut(s) 216, 830, 1108, 2175
TseFI GTSAC 4 cut(s) 531, 664, 1058, 1977
TseI GCWGC 1 cut(s) 1454
Tsp45I GTSAC 4 cut(s) 531, 664, 1058, 1977
TspGWI ACGGA 3 cut(s) 1938, 2346, 2385
TspRI CASTG 4 cut(s) 216, 830, 1108, 2175
Van91I CCANNNNNTGG 1 cut(s) 66
VpaK11BI GGWCC 4 cut(s) 349, 541, 1743, 2245
XapI RAATTY 6 cut(s) 122, 151, 174, 400, 1589, 1890
XbaI TCTAGA 3 cut(s) 505, 1867, 2086
XceI RCATGY 4 cut(s) 1338, 1699, 1978, 2366
XcmI CCANNNNNNNNNTGG 2 cut(s) 127, 1499
XmiI GTMKAC 1 cut(s) 329
XmnI GAANNNNTTC 3 cut(s) 1093, 2335, 2377
Zsp2I ATGCAT 1 cut(s) 2207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.