MD05G1137400.v1.1

Toll - interleukin 1 - resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000020
Physical Location & Seq
Reverse (-)
26545955 .. 26549189
3235 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1137400.v1.1.491

Sequence Viewer

Length: 2319 bp
ATGGAGTTCATCCAAGTTTTCATCCTCATCGCCATTGCCATCGGGACTCTGTTGTACATGTGCTGCAGATCATTAACATTCTCTTCTTCGTCTTCTTCTTCTTCATCTACTGCGGCTGATTCTACTGCTGTTGCTGATGATGATGATGCTGATAGCCCCCCACGTCGAGAAAAGTATGATGTGTTTATCAGCTTCAGAGGTCAGGACACCCGCAATACATTTACCAGCCATCTTCATGCTGCCTTACTTCAGAAGAACATTGAAACCTACATAGATAACAGACTTCAGAGAGGAGAAGAAATTATACCTGCTGTTCTAGAGGCAATCGAGAAATCAACGGTTTGGGTGATCATTTTCTCACAAAACTATGCTTCTTCCACATGGTGTTTGGATGAAATTGTGCATATACTGAAATGTTACGGAAGAGGAGGCGGCTATGTGATACCCATTTTTTACCACACCAATCCGTCAGATGTACGAAAACAAGGCGGGAGTTATGCGGTTGCATTTGCCCAACACCAAAAACGTTTCAAGGACAGTATCGACAAGGTGCACGAGTGGAGGAAGGCTTTGTGGAATGCAACCAATTTCTCTGGGTTTGATTGCTATTATGGACGTACAGAGGCAGATCTAATTGAGGCAGTTGTCAATCATATTTGTACCAGATTGAGTTTTGAATCATCATGTGTTTTACAGGGCTTGGTTGGAATTGAAAGCCGCATCGAACAAATTGAAGAGCTATTGGACATTCATTCACCGGACGCTTGCATCACTACTGTAGGTATTTGGGGCATGGGTGGTGTTGGCAAGACCACCCTTGCTGAAACTGTATTTCACAAACTCTCTTCTAAATTCGAAGCGAGCTGTTTTCTTAGAAATGTCAGGGAGTACTCAGAACAAGGACATGGACTATATCACTTGGAAGAAACACTTCTTAAGGAGATGTTTAAGGAAGAAGTTCTGCCATCAACTTTTATTCGAAAAAGGCTCAGCCGCACAAAGGTCCTTATTGTTCTTGATGATGTGAGTAGTTCAATGCAAGTGGAACAATTAGTTGGCGATCGTTTTAATTATGGCAGTGGAAGTAGAATCATTATCACAAGTAGAGATAGGGGCGCACTTAGGAATACTGTTGAAGATGATAAGATCTACAAGGTTGAAGGATTAAAACCGGATGATGCTCTTCAGCTCTTCTGTTCGCGTGCTTTCAAGAATACTCGTATACATCTGGGGTTGGCAGAAAAGGCAATGCATTATGCAGGAGGCATTCCTTTAGCTCTTATACATCTGGGGTCCTCATTCCTAAGTTGCAAGAGCAAAGAAGACATGGAAGATGAATTGGACAAATTGAAACAATTTCCCAATGAAAATATCCAGAGAGTGTTGAGACTAAGTTATGATAGATTGGATAGCAATGAGAAGGGGATATTTTTGGATATAGCATGTTTTCATATAGGGGAGAATGTGAAGAAGGTAGAACAATTGTTAGATATTCGTAGATTCTATACGGTGGTCGGAATTAGCATTCTCATTGATATGTCTCTCATATCAATCGGTTCAAAATGGGGACGGGAAACCATCGAGATGCATAATCTGTTTGAAGAAATGGGAAGGACAATTGTTCAGGAACAAAGTATTGGAAATCCCAGCAATTGGAGTAGGTTGTTCAACGATAAGGATGTTTATCATGTATTGAAAAGTAACACGGAAACTCCAAAGGTTGAAGCCATACGGGTTAATTGGTCTAAGATTGAAGAGCGACCATTGAAATGTGCAGACTTCAAAAAGATGTCTAACTTGACAATGCTAATTGTGGACGCTGGCTACAAATTCAACGCTTCTCTAGACCTTCCCGATTCTCTTCTTTATCTTTACTGGGAAGGATATCCACTGGAATCTTTGCCATCAAATTTTTGTCCAGAAGATCTAGTTGAGCTTCATATGCCATATAGCAAAGTTAAGAAGCTTTGGAAAGAAGAGCAGAGACTTGTGAACTTACAAGTGATCGATCTGCATGACTCAACAAATCTAACTGAAGTTCCAAATCTCTATGCGAGTCTAAAAATTGTGAACATAAATCTCTCTGGCTGTGAAAGTTTGGTTGAAATCCCTGAAGGTCTCGTTTGCTCAACCTCATTGGAATTTTTGGATCTGTCTGGAACCAAGATTAGGAGCATACCTGCAAGCATCAAACAAGCTTCTCGGTTGTCTAAACTCAACATAGTCAACTGCAAGTGGCTTCAATCTTTGCCAGAACTCCCACTGCAATGTAATGTCGAAGCTGAAGGCTGCACGGCGCTGAAGGCTGCACGGCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

773

Amino Acids

87.39

Weight (kDa)

5.84

Isoelectric Point (pI)

53.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 58 - 237 1.1e-48 TIR domain
TIR_2 PF13676 61 - 181 6.4e-15 TIR domain
NB-ARC PF00931 239 - 406 6.4e-21 NB-ARC domain
NACHT PF05729 259 - 399 3.5e-09 NACHT domain
WHD_ROQ1 PF23282 471 - 545 4.9e-17 Disease resistance protein Roq1-like, winged-helix domain
LRR_3 PF07725 642 - 661 8.6e-07 Leucine Rich Repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000020)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g26600 FvH4_2g27070 FvH4_2g27070 FvH4_2g27070 FvH4_2g27370 FvH4_2g27370 FvH4_2g27370 FvH4_2g27370 FvH4_2g27370 FvH4_2g27370 FvH4_2g27370 FvH4_2g27370 FvH4_2g27701 FvH4_2g27720 FvH4_3g05740 FvH4_3g05740 FvH4_3g05740 FvH4_3g05740 FvH4_3g15720 FvH4_5g35770 FvH4_5g35770 FvH4_5g35770 FvH4_5g35770 FvH4_5g35770 FvH4_6g34390 FvH4_6g34410 FvH4_6g34410
malus_domestica MD02G1278900.v1.1 MD03G1127300.v1.1 MD04G1027100.v1.1 MD04G1035000.v1.1 MD04G1035200.v1.1 MD04G1035600.v1.1 MD04G1036000.v1.1 MD04G1036100.v1.1 MD04G1036400.v1.1 MD05G1125400.v1.1 MD05G1125600.v1.1 MD05G1125700.v1.1 MD05G1126000.v1.1 MD05G1131900.v1.1 MD05G1132400.v1.1 MD05G1132600.v1.1 MD05G1132800.v1.1 MD05G1135700.v1.1 MD05G1135800.v1.1 MD05G1136700.v1.1 MD05G1137400.v1.1 MD05G1137900.v1.1 MD05G1138300.v1.1 MD05G1138400.v1.1 MD05G1138500.v1.1 MD05G1141700.v1.1 MD05G1142600.v1.1 MD05G1143300.v1.1 MD05G1145200.v1.1 MD05G1146500.v1.1 MD05G1146600.v1.1 MD05G1147100.v1.1 MD05G1147500.v1.1 MD05G1147600.v1.1 MD05G1148300.v1.1 MD05G1148600.v1.1 MD05G1149400.v1.1 MD05G1149500.v1.1 MD05G1150100.v1.1 MD05G1150200.v1.1 MD05G1150900.v1.1 MD05G1151200.v1.1 MD08G1168700.v1.1 MD10G1128400.v1.1 MD10G1128500.v1.1 MD10G1128600.v1.1 MD10G1134800.v1.1 MD10G1135300.v1.1 MD10G1135800.v1.1 MD10G1135900.v1.1 MD10G1136100.v1.1 MD10G1136200.v1.1 MD10G1136400.v1.1 MD10G1136500.v1.1 MD10G1136600.v1.1 MD10G1137100.v1.1 MD10G1137400.v1.1 MD10G1137500.v1.1 MD10G1137700.v1.1 MD10G1140900.v1.1 MD10G1141100.v1.1 MD10G1141400.v1.1 MD12G1073200.v1.1 MD12G1074900.v1.1 MD16G1140700.v1.1
prunus_persica Prupe.1G160400_v2.0.a1 Prupe.1G160500_v2.0.a1 Prupe.2G060400_v2.0.a1 Prupe.2G060400_v2.0.a1 Prupe.2G060400_v2.0.a1 Prupe.2G060400_v2.0.a1 Prupe.2G060400_v2.0.a1 Prupe.2G060400_v2.0.a1 Prupe.2G083200_v2.0.a1 Prupe.6G152300_v2.0.a1 Prupe.8G037000_v2.0.a1 Prupe.8G117300_v2.0.a1 Prupe.8G117700_v2.0.a1 Prupe.8G118900_v2.0.a1 Prupe.8G173600_v2.0.a1 Prupe.8G173700_v2.0.a1 Prupe.8G176400_v2.0.a1 Prupe.8G179400_v2.0.a1 Prupe.8G179500_v2.0.a1 Prupe.8G179500_v2.0.a1 Prupe.8G179500_v2.0.a1 Prupe.8G179500_v2.0.a1 Prupe.8G179500_v2.0.a1 Prupe.8G179500_v2.0.a1 Prupe.8G179500_v2.0.a1 Prupe.8G179500_v2.0.a1 Prupe.8G179500_v2.0.a1 Prupe.8G179500_v2.0.a1 Prupe.8G179500_v2.0.a1 Prupe.8G237200_v2.0.a1 Prupe.I001800_v2.0.a1 Prupe.I002000_v2.0.a1 Prupe.I002100_v2.0.a1 Prupe.I002200_v2.0.a1 Prupe.I002300_v2.0.a1 Prupe.I002300_v2.0.a1 Prupe.I002300_v2.0.a1
pyrus_communis pycom02g10490 pycom03g08410 pycom04g02350 pycom04g02370 pycom04g02890 pycom04g02920 pycom04g02930 pycom05g09430 pycom05g11970 pycom05g11980 pycom05g12000 pycom05g12010 pycom05g12040 pycom05g12080 pycom05g12100 pycom05g12110 pycom05g12160 pycom05g12910 pycom05g12920 pycom05g12940 pycom05g13220 pycom05g13230 pycom05g13260 pycom05g13300 pycom05g13320 pycom05g13330 pycom05g13350 pycom05g13680 pycom05g13710 pycom05g13720 pycom05g13730 pycom05g14120 pycom05g14130 pycom05g14160 pycom05g14210 pycom05g14250 pycom05g14280 pycom05g14290 pycom05g14340 pycom05g14390 pycom05g14430 pycom05g14460 pycom05g14530 pycom05g14560 pycom05g14570 pycom05g14580 pycom10g11010 pycom10g11020 pycom10g11030 pycom10g11250 pycom10g11640 pycom10g11670 pycom10g11680 pycom10g11720 pycom10g11730 pycom10g11950 pycom10g11960 pycom10g12000 pycom10g12050 pycom10g12070 pycom10g12310
rosa_chinensis RchiOBHm_Chr1g0314371 RchiOBHm_Chr1g0314461 RchiOBHm_Chr1g0314491 RchiOBHm_Chr1g0314661 RchiOBHm_Chr1g0314671 RchiOBHm_Chr1g0314701 RchiOBHm_Chr1g0314711 RchiOBHm_Chr1g0314741 RchiOBHm_Chr1g0327611 RchiOBHm_Chr1g0333931 RchiOBHm_Chr1g0334091 RchiOBHm_Chr1g0334101 RchiOBHm_Chr2g0144731 RchiOBHm_Chr2g0144751 RchiOBHm_Chr3g0451471 RchiOBHm_Chr3g0451481 RchiOBHm_Chr3g0451521 RchiOBHm_Chr3g0451541 RchiOBHm_Chr3g0451581 RchiOBHm_Chr3g0451641 RchiOBHm_Chr3g0451661 RchiOBHm_Chr3g0451671 RchiOBHm_Chr3g0484861 RchiOBHm_Chr5g0013861 RchiOBHm_Chr5g0013871 RchiOBHm_Chr5g0013881 RchiOBHm_Chr5g0013921 RchiOBHm_Chr5g0013931 RchiOBHm_Chr5g0026001 RchiOBHm_Chr5g0026101 RchiOBHm_Chr5g0026421 RchiOBHm_Chr5g0066271 RchiOBHm_Chr6g0287411 RchiOBHm_Chr6g0287421 RchiOBHm_Chr6g0287561 RchiOBHm_Chr6g0287581 RchiOBHm_Chr6g0287591 RchiOBHm_Chr6g0296221 RchiOBHm_Chr7g0237431
rosa_laevigata RLG00000001003 RLG00000011679 RLG00000011716 RLG00000012483 RLG00000012484 RLG00000013019 RLG00000015060 RLG00000020104 RLG00000020105 RLG00000020106 RLG00000020107 RLG00000020108 RLG00000020109 RLG00000023202 RLG00000024217 RLG00000025656 RLG00000025657 RLG00000026224 RLG00000029504 RLG00000029507 RLG00000029508 RLG00000029509 RLG00000029980 RLG00000030042 RLG00000032050 RLG00000032051 RLG00000032052 RLG00000032891 RLG00000032896 RLG00000032900 RLG00000032901 RLG00000032930 RLG00000032931 RLG00000035381 RLG00000035383 RLG00000035847
rosa_multiflora Rmu_co7983036.1_g000001 Rmu_co8026080.1_g000001 Rmu_co8242645.1_g000001 Rmu_co8396077.1_g000001 Rmu_co8406137.1_g000001 Rmu_co8420673.1_g000001 Rmu_co8458221.1_g000001 Rmu_sc0000026.1_g000007 Rmu_sc0000795.1_g000054 Rmu_sc0000997.1_g000011 Rmu_sc0001689.1_g000014 Rmu_sc0001802.1_g000009 Rmu_sc0002354.1_g000053 Rmu_sc0002354.1_g000055 Rmu_sc0002806.1_g000016 Rmu_sc0002833.1_g000022 Rmu_sc0002843.1_g000009 Rmu_sc0002923.1_g000021 Rmu_sc0003020.1_g000017 Rmu_sc0003598.1_g000012 Rmu_sc0003598.1_g000013 Rmu_sc0003598.1_g000014 Rmu_sc0003598.1_g000015 Rmu_sc0003690.1_g000001 Rmu_sc0004708.1_g000014 Rmu_sc0004924.1_g000009 Rmu_sc0004924.1_g000018 Rmu_sc0004924.1_g000022 Rmu_sc0004924.1_g000026 Rmu_sc0004924.1_g000032 Rmu_sc0004924.1_g000033 Rmu_sc0004924.1_g000037 Rmu_sc0004924.1_g000039 Rmu_sc0004974.1_g000006 Rmu_sc0005581.1_g000001 Rmu_sc0005581.1_g000010 Rmu_sc0005803.1_g000021 Rmu_sc0006653.1_g000009 Rmu_sc0007029.1_g000008 Rmu_sc0009360.1_g000007 Rmu_sc0009360.1_g000012 Rmu_sc0009360.1_g000013 Rmu_sc0009360.1_g000015 Rmu_sc0009360.1_g000021 Rmu_sc0009360.1_g000033 Rmu_sc0009360.1_g000048 Rmu_sc0009360.1_g000049 Rmu_sc0009360.1_g000051 Rmu_sc0011096.1_g000005 Rmu_sc0016584.1_g000002 Rmu_sc0020815.1_g000001 Rmu_sc0020815.1_g000002 Rmu_sc0025510.1_g000001 Rmu_sc0026263.1_g000003 Rmu_sc0029456.1_g000001 Rmu_sc0029457.1_g000001 Rmu_sc0029568.1_g000001 Rmu_sc0032214.1_g000001 Rmu_sc0041321.1_g000001 Rmu_sc0042928.1_g000001 Rmu_ssc0000042.1_g000010 Rmu_ssc0000042.1_g000019 Rmu_ssc0000042.1_g000020 Rmu_ssc0000126.1_g000058
rosa_roxburghii Rroxscaffold_164G00436360 Rroxscaffold_164G00436400 Rroxscaffold_164G00436430 Rroxscaffold_164G00436440 Rroxscaffold_164G00436470 Rroxscaffold_1G00052970 Rroxscaffold_1G00062790 Rroxscaffold_1G00062820 Rroxscaffold_2G00100990 Rroxscaffold_2G00101010 Rroxscaffold_3G00224210 Rroxscaffold_4G00297610 Rroxscaffold_4G00317070 Rroxscaffold_4G00317100 Rroxscaffold_4G00317110 Rroxscaffold_4G00317120 Rroxscaffold_4G00322520 Rroxscaffold_4G00322530 Rroxscaffold_4G00323280 Rroxscaffold_4G00331920 Rroxscaffold_6G00410350 Rroxscaffold_6G00428880 Rroxscaffold_6G00428910 Rroxscaffold_6G00428930 Rroxscaffold_7G00171360 Rroxscaffold_7G00171730 Rroxscaffold_7G00178330 Rroxscaffold_7G00178730 Rroxscaffold_7G00180960 Rroxscaffold_7G00181370
rosa_rugosa Rorug01G0001400 Rorug01G0001500 Rorug01G0001600 Rorug01G0001700 Rorug01G0068600 Rorug01G0068700 Rorug02G0389400 Rorug02G0389500 Rorug02G0634000 Rorug02G0634300 Rorug02G0634600 Rorug02G0634800 Rorug02G0634900 Rorug02G0634900 Rorug02G0635000 Rorug02G0635100 Rorug02G0635200 Rorug02G0635300 Rorug02G0635400 Rorug03G0213900 Rorug05G0012800 Rorug05G0012900 Rorug05G0013100 Rorug05G0013300 Rorug05G0090300 Rorug05G0090400 Rorug05G0092900 Rorug05G0093000 Rorug05G0093100 Rorug06G0189200 Rorug06G0189200 Rorug06G0189300 Rorug06G0189400 Rorug06G0259500 Rorug07G0303200 Rorug07G0303300.1
rosa_samantha Rh1AG010600 Rh1AG010900 Rh1AG011800 Rh1AG012100 Rh1AG012500 Rh1AG081000 Rh1AG085900 Rh1AG135300 Rh1AG135400 Rh1AG135500 Rh1AG135700 Rh1AG135800 Rh1AG136400 Rh1BG003400 Rh1BG003700 Rh1BG068500 Rh1BG103400 Rh1BG103500 Rh1BG103600 Rh1CG128600 Rh1CG128800 Rh1DG005800 Rh1DG090100 Rh1DG140800 Rh1DG140900 Rh1DG141100 Rh1DG141200 Rh1DG141500 Rh2AG441200 Rh2BG451500 Rh2BG451800 Rh2BG451900 Rh2CG428500 Rh2DG461500 Rh3AG037000 Rh3AG037100 Rh3AG037200 Rh3BG037600 Rh3BG037700 Rh3BG037800 Rh3BG038100 Rh3BG299500 Rh3BG299800 Rh3BG299900 Rh3CG036200 Rh3CG036400 Rh3CG036500 Rh3CG036800 Rh3CG297200 Rh3DG037100 Rh3DG037200 Rh3DG037400 Rh3DG293200 Rh5BG103800 Rh5BG104100 Rh5BG104200 Rh5BG180100 Rh5BG180200 Rh5BG183000 Rh5BG451600 Rh5CG115600 Rh5CG115700 Rh5CG115900 Rh5CG116100 Rh5CG198800 Rh5CG199300 Rh5CG202400 Rh5DG102100 Rh5DG102200 Rh5DG102300 Rh5DG102600 Rh5DG102800 Rh5DG181100 Rh5DG181300 Rh5DG181800 Rh5DG184900 Rh5DG185100 Rh6AG256000 Rh6AG299200 Rh6AG299300 Rh6AG300400 Rh6AG371800 Rh6BG383300 Rh6CG303400 Rh6CG314400 Rh6CG385000 Rh6DG297800 Rh6DG372300 Rh7BG046900 Rh7BG312300 Rh7BG312500 Rh7BG430400
rosa_wichuraiana Rw0G009620 Rw0G015730 Rw1G000490 Rw1G006410 Rw1G006700 Rw1G011270 Rw1G011280 Rw1G011310 Rw2G036080 Rw3G002740 Rw3G002750 Rw3G002760 Rw3G002770 Rw3G023580 Rw5G009360 Rw5G009370 Rw5G009380 Rw5G009390 Rw5G009400 Rw5G016550 Rw5G016890 Rw5G016910 Rw5G040760 Rw6G022190 Rw6G025910 Rw6G032400 Rw6G032690 Rw6G032790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 316, 2188
AccB7I CCANNNNNTGG 1 cut(s) 1653
AccI GTMKAC 1 cut(s) 1222
AccII CGCG 1 cut(s) 1201
AciI CCGC 7 cut(s) 113, 211, 432, 489, 500, 718, 994
AclI AACGTT 1 cut(s) 526
AclWI GGATC 1 cut(s) 2157
AcsI RAATTY 4 cut(s) 851, 1829, 1909, 2141
AcuI CTGAAG 7 cut(s) 178, 233, 269, 1169, 2055, 2133, 2304
AdeI CACNNNGTG 1 cut(s) 384
AfaI GTAC 5 cut(s) 56, 477, 619, 661, 890
AfiI CCNNNNNNNGG 3 cut(s) 1000, 1653, 2169
AflII CTTAAG 1 cut(s) 935
AflIII ACRYGT 1 cut(s) 57
AjiI CACGTC 1 cut(s) 164
AjuI GAANNNNNNNTTGG 4 cut(s) 1038, 1070, 1620, 1652
AluBI AGCT 9 cut(s) 192, 739, 864, 1189, 1277, 1936, 1966, 2198, 2282
AluI AGCT 9 cut(s) 192, 739, 864, 1189, 1277, 1936, 1966, 2198, 2282
Alw21I GWGCWC 1 cut(s) 555
Alw26I GTCTC 4 cut(s) 1381, 1545, 1978, 2123
Alw44I GTGCAC 1 cut(s) 551
AlwI GGATC 1 cut(s) 2157
ApaLI GTGCAC 1 cut(s) 551
ApeKI GCWGC 4 cut(s) 63, 239, 2289, 2306
ApoI RAATTY 4 cut(s) 851, 1829, 1909, 2141
Asp700I GAANNNNTTC 2 cut(s) 930, 957
AspLEI GCGC 3 cut(s) 1118, 2299, 2316
AspS9I GGNCC 2 cut(s) 1003, 1293
AsuHPI GGTGA 2 cut(s) 358, 747
AsuII TTCGAA 2 cut(s) 855, 979
AvaII GGWCC 2 cut(s) 1003, 1293
BaeGI GKGCMC 1 cut(s) 555
BarI GAAGNNNNNNTAC 2 cut(s) 288, 320
BauI CACGAG 1 cut(s) 554
BbsI GAAGAC 2 cut(s) 84, 1329
Bbv12I GWGCWC 1 cut(s) 555
BbvI GCAGC 4 cut(s) 50, 226, 2276, 2293
BccI CCATC 5 cut(s) 47, 237, 973, 1586, 1912
BceAI ACGGC 1 cut(s) 2310
BclI TGATCA 1 cut(s) 348
BcoDI GTCTC 4 cut(s) 1381, 1545, 1978, 2123
BfaI CTAG 3 cut(s) 317, 1844, 1928
BfmI CTRYAG 2 cut(s) 64, 777
BfoI RGCGCY 2 cut(s) 2300, 2317
BfrI CTTAAG 1 cut(s) 935
BfuAI ACCTGC 2 cut(s) 316, 2188
BglII AGATCT 3 cut(s) 628, 1146, 1924
BisI GCNGC 8 cut(s) 64, 114, 240, 433, 718, 994, 2290, 2307
BlpI GCTNAGC 1 cut(s) 989
BlsI GCNGC 8 cut(s) 65, 115, 241, 434, 719, 995, 2291, 2308
BmcAI AGTACT 1 cut(s) 890
Bme18I GGWCC 2 cut(s) 1003, 1293
BmgBI CACGTC 1 cut(s) 164
BmgT120I GGNCC 2 cut(s) 1003, 1293
BmiI GGNNCC 2 cut(s) 1294, 2161
BmrI ACTGGG 1 cut(s) 1885
BmsI GCATC 6 cut(s) 136, 729, 777, 1168, 1575, 2196
BmuI ACTGGG 1 cut(s) 1885
BpiI GAAGAC 2 cut(s) 84, 1329
Bpu1102I GCTNAGC 1 cut(s) 989
Bpu14I TTCGAA 2 cut(s) 855, 979
Bsa29I ATCGAT 1 cut(s) 2007
BsaBI GATNNNNATC 1 cut(s) 1683
BsaI GGTCTC 1 cut(s) 2123
BsaWI WCCGGW 2 cut(s) 757, 1171
BsaXI ACNNNNNCTCC 2 cut(s) 1696, 1726
Bsc4I CCNNNNNNNGG 3 cut(s) 1000, 1653, 2169
Bse1I ACTGG 2 cut(s) 1880, 1896
Bse3DI GCAATG 4 cut(s) 33, 1254, 1420, 2273
Bse8I GATNNNNATC 1 cut(s) 1683
BseCI ATCGAT 1 cut(s) 2007
BseGI GGATG 5 cut(s) 9, 21, 397, 1180, 1684
BseJI GATNNNNATC 1 cut(s) 1683
BseLI CCNNNNNNNGG 3 cut(s) 1000, 1653, 2169
BseMI GCAATG 4 cut(s) 33, 1254, 1420, 2273
BseMII CTCAG 2 cut(s) 906, 1003
BseNI ACTGG 2 cut(s) 1880, 1896
BseRI GAGGAG 2 cut(s) 306, 441
BseSI GKGCMC 1 cut(s) 555
BseXI GCAGC 4 cut(s) 50, 226, 2276, 2293
BseYI CCCAGC 1 cut(s) 1646
BsgI GTGCAG 3 cut(s) 1794, 2275, 2292
Bsh1236I CGCG 1 cut(s) 1201
Bsh1285I CGRYCG 1 cut(s) 1063
BshVI ATCGAT 1 cut(s) 2007
BsiEI CGRYCG 1 cut(s) 1063
BsiHKAI GWGCWC 1 cut(s) 555
BsiSI CCGG 2 cut(s) 758, 1172
BslFI GGGAC 2 cut(s) 58, 1581
BslI CCNNNNNNNGG 3 cut(s) 1000, 1653, 2169
BsmAI GTCTC 4 cut(s) 1381, 1545, 1978, 2123
BsmFI GGGAC 2 cut(s) 58, 1581
BsmI GAATGC 3 cut(s) 583, 1266, 1524
Bso31I GGTCTC 1 cut(s) 2123
Bsp119I TTCGAA 2 cut(s) 855, 979
Bsp1286I GDGCHC 1 cut(s) 555
Bsp1407I TGTACA 1 cut(s) 54
Bsp143I GATC 9 cut(s) 68, 348, 628, 1060, 1146, 1924, 2004, 2008, 2149
Bsp1720I GCTNAGC 1 cut(s) 989
BspACI CCGC 7 cut(s) 113, 211, 432, 489, 500, 718, 994
BspCNI CTCAG 2 cut(s) 905, 1002
BspDI ATCGAT 1 cut(s) 2007
BspFNI CGCG 1 cut(s) 1201
BspLI GGNNCC 2 cut(s) 1294, 2161
BspMAI CTGCAG 1 cut(s) 68
BspMI ACCTGC 2 cut(s) 316, 2188
BspPI GGATC 1 cut(s) 2157
BspQI GCTCTTC 5 cut(s) 729, 1188, 1196, 1749, 1971
BspT104I TTCGAA 2 cut(s) 855, 979
BspTI CTTAAG 1 cut(s) 935
BspTNI GGTCTC 1 cut(s) 2123
BsrDI GCAATG 4 cut(s) 33, 1254, 1420, 2273
BsrGI TGTACA 1 cut(s) 54
BsrI ACTGG 2 cut(s) 1880, 1896
BssMI GATC 9 cut(s) 68, 348, 628, 1060, 1146, 1924, 2004, 2008, 2149
BssNAI GTATAC 1 cut(s) 1223
BssSI CACGAG 1 cut(s) 554
Bst1107I GTATAC 1 cut(s) 1223
Bst2BI CACGAG 1 cut(s) 554
Bst4CI ACNGT 6 cut(s) 340, 539, 778, 829, 1132, 1510
Bst6I CTCTTC 9 cut(s) 88, 418, 729, 850, 1188, 1196, 1749, 1866, 1971
BstAFI CTTAAG 1 cut(s) 935
BstAUI TGTACA 1 cut(s) 54
BstBI TTCGAA 2 cut(s) 855, 979
BstC8I GCNNGC 5 cut(s) 766, 862, 1203, 1822, 2185
BstDEI CTNAG 7 cut(s) 872, 892, 989, 1121, 1304, 1391, 1746
BstF5I GGATG 5 cut(s) 9, 21, 397, 1180, 1684
BstFNI CGCG 1 cut(s) 1201
BstH2I RGCGCY 2 cut(s) 2300, 2317
BstHHI GCGC 3 cut(s) 1118, 2299, 2316
BstKTI GATC 9 cut(s) 71, 351, 631, 1063, 1149, 1927, 2007, 2011, 2152
BstMAI GTCTC 4 cut(s) 1381, 1545, 1978, 2123
BstMBI GATC 9 cut(s) 68, 348, 628, 1060, 1146, 1924, 2004, 2008, 2149
BstMCI CGRYCG 1 cut(s) 1063
BstMWI GCNNNNNNNGC 3 cut(s) 1244, 1942, 2303
BstNSI RCATGY 2 cut(s) 61, 1446
BstSFI CTRYAG 2 cut(s) 64, 777
BstSLI GKGCMC 1 cut(s) 555
BstUI CGCG 1 cut(s) 1201
BstV1I GCAGC 4 cut(s) 50, 226, 2276, 2293
BstV2I GAAGAC 2 cut(s) 84, 1329
BstX2I RGATCY 4 cut(s) 628, 1146, 1924, 2149
BstYI RGATCY 4 cut(s) 628, 1146, 1924, 2149
BstZ17I GTATAC 1 cut(s) 1223
Bsu15I ATCGAT 1 cut(s) 2007
BsuTUI ATCGAT 1 cut(s) 2007
BtgZI GCGATG 1 cut(s) 13
BtrI CACGTC 1 cut(s) 164
BtsCI GGATG 5 cut(s) 9, 21, 397, 1180, 1684
BtsI GCAGTG 2 cut(s) 1084, 2261
BtsIMutI CAGTG 3 cut(s) 1084, 1889, 2261
BveI ACCTGC 2 cut(s) 316, 2188
Cac8I GCNNGC 5 cut(s) 766, 862, 1203, 1822, 2185
CfoI GCGC 3 cut(s) 1118, 2299, 2316
Cfr13I GGNCC 2 cut(s) 1003, 1293
ClaI ATCGAT 1 cut(s) 2007
CseI GACGC 2 cut(s) 770, 1826
Csp6I GTAC 5 cut(s) 55, 476, 618, 660, 889
CspCI CAANNNNNGTGG 2 cut(s) 1023, 1058
CviQI GTAC 5 cut(s) 55, 476, 618, 660, 889
DdeI CTNAG 7 cut(s) 872, 892, 989, 1121, 1304, 1391, 1746
DpnI GATC 9 cut(s) 70, 350, 630, 1062, 1148, 1926, 2006, 2010, 2151
DpnII GATC 9 cut(s) 68, 348, 628, 1060, 1146, 1924, 2004, 2008, 2149
DraIII CACNNNGTG 1 cut(s) 384
Eam1104I CTCTTC 9 cut(s) 88, 418, 729, 850, 1188, 1196, 1749, 1866, 1971
EarI CTCTTC 9 cut(s) 88, 418, 729, 850, 1188, 1196, 1749, 1866, 1971
Eco31I GGTCTC 1 cut(s) 2123
Eco32I GATATC 1 cut(s) 1886
Eco47I GGWCC 2 cut(s) 1003, 1293
Eco57I CTGAAG 7 cut(s) 178, 233, 269, 1169, 2055, 2133, 2304
EcoO109I RGGNCCY 2 cut(s) 1003, 1293
EcoRV GATATC 1 cut(s) 1886
EcoT22I ATGCAT 2 cut(s) 1254, 1590
FalI AAGNNNNNCTT 2 cut(s) 915, 947
FaqI GGGAC 2 cut(s) 58, 1581
FauI CCCGC 2 cut(s) 218, 482
FauNDI CATATG 1 cut(s) 1941
FbaI TGATCA 1 cut(s) 348
FblI GTMKAC 1 cut(s) 1222
Fnu4HI GCNGC 8 cut(s) 64, 114, 240, 433, 718, 994, 2290, 2307
FokI GGATG 4 cut(s) 8, 404, 1187, 1691
Fsp4HI GCNGC 8 cut(s) 64, 114, 240, 433, 718, 994, 2290, 2307
FspBI CTAG 3 cut(s) 317, 1844, 1928
GlaI GCGC 3 cut(s) 1117, 2298, 2315
GluI GCNGC 8 cut(s) 64, 114, 240, 433, 718, 994, 2290, 2307
GsaI CCCAGC 1 cut(s) 1650
HaeII RGCGCY 2 cut(s) 2300, 2317
HapII CCGG 2 cut(s) 758, 1172
HgaI GACGC 2 cut(s) 770, 1826
HhaI GCGC 3 cut(s) 1118, 2299, 2316
Hin6I GCGC 3 cut(s) 1116, 2297, 2314
HinP1I GCGC 3 cut(s) 1116, 2297, 2314
HincII GTYRAC 1 cut(s) 2227
HindII GTYRAC 1 cut(s) 2227
HindIII AAGCTT 2 cut(s) 1964, 2196
HinfI GANTC 9 cut(s) 46, 119, 677, 1089, 1500, 1856, 1895, 2018, 2056
HpaII CCGG 2 cut(s) 758, 1172
HphI GGTGA 2 cut(s) 358, 747
Hpy166II GTNNAC 6 cut(s) 553, 1223, 1816, 1993, 2071, 2227
Hpy188I TCNGA 6 cut(s) 197, 252, 288, 472, 895, 1517
Hpy8I GTNNAC 6 cut(s) 553, 1223, 1816, 1993, 2071, 2227
Hpy99I CGWCG 1 cut(s) 168
HpyCH4III ACNGT 6 cut(s) 340, 539, 778, 829, 1132, 1510
HpyCH4IV ACGT 3 cut(s) 163, 526, 616
HpyF10VI GCNNNNNNNGC 3 cut(s) 1244, 1942, 2303
HpyF3I CTNAG 7 cut(s) 872, 892, 989, 1121, 1304, 1391, 1746
HpySE526I ACGT 3 cut(s) 163, 526, 616
HspAI GCGC 3 cut(s) 1116, 2297, 2314
Ksp22I TGATCA 1 cut(s) 348
Kzo9I GATC 9 cut(s) 68, 348, 628, 1060, 1146, 1924, 2004, 2008, 2149
LguI GCTCTTC 5 cut(s) 729, 1188, 1196, 1749, 1971
LmnI GCTCC 1 cut(s) 2172
Lsp1109I GCAGC 4 cut(s) 50, 226, 2276, 2293
LweI GCATC 6 cut(s) 136, 729, 777, 1168, 1575, 2196
MaeI CTAG 3 cut(s) 317, 1844, 1928
MaeII ACGT 3 cut(s) 163, 526, 616
MaeIII GTNAC 2 cut(s) 416, 1700
MalI GATC 9 cut(s) 70, 350, 630, 1062, 1148, 1926, 2006, 2010, 2151
MboI GATC 9 cut(s) 68, 348, 628, 1060, 1146, 1924, 2004, 2008, 2149
MfeI CAATTG 3 cut(s) 1481, 1617, 1651
MflI RGATCY 4 cut(s) 628, 1146, 1924, 2149
MhlI GDGCHC 1 cut(s) 555
MlyI GAGTC 3 cut(s) 40, 2012, 2065
MmeI TCCRAC 2 cut(s) 685, 1495
Mph1103I ATGCAT 2 cut(s) 1254, 1590
MroXI GAANNNNTTC 2 cut(s) 930, 957
MseI TTAA 7 cut(s) 74, 936, 948, 1068, 1166, 1737, 1959
MslI CAYNNNNRTG 5 cut(s) 234, 1535, 1583, 1768, 2266
MspCI CTTAAG 1 cut(s) 935
MspI CCGG 2 cut(s) 758, 1172
MunI CAATTG 3 cut(s) 1481, 1617, 1651
Mva1269I GAATGC 3 cut(s) 583, 1266, 1524
MvnI CGCG 1 cut(s) 1201
MwoI GCNNNNNNNGC 3 cut(s) 1244, 1942, 2303
NdeI CATATG 1 cut(s) 1941
NdeII GATC 9 cut(s) 68, 348, 628, 1060, 1146, 1924, 2004, 2008, 2149
NlaIV GGNNCC 2 cut(s) 1294, 2161
NsiI ATGCAT 2 cut(s) 1254, 1590
NspI RCATGY 2 cut(s) 61, 1446
NspV TTCGAA 2 cut(s) 855, 979
PciI ACATGT 1 cut(s) 57
PciSI GCTCTTC 5 cut(s) 729, 1188, 1196, 1749, 1971
PctI GAATGC 3 cut(s) 583, 1266, 1524
PdmI GAANNNNTTC 2 cut(s) 930, 957
PfeI GAWTC 6 cut(s) 119, 677, 1089, 1500, 1856, 1895
PflMI CCANNNNNTGG 1 cut(s) 1653
PkrI GCNGC 8 cut(s) 65, 115, 241, 434, 719, 995, 2291, 2308
Ple19I CGATCG 1 cut(s) 1063
PleI GAGTC 3 cut(s) 40, 2012, 2064
PpsI GAGTC 3 cut(s) 40, 2012, 2064
PpuMI RGGWCCY 2 cut(s) 1003, 1293
PscI ACATGT 1 cut(s) 57
Psp1406I AACGTT 1 cut(s) 526
Psp5II RGGWCCY 2 cut(s) 1003, 1293
PspFI CCCAGC 1 cut(s) 1646
PspN4I GGNNCC 2 cut(s) 1294, 2161
PspPI GGNCC 2 cut(s) 1003, 1293
PspPPI RGGWCCY 2 cut(s) 1003, 1293
PstI CTGCAG 1 cut(s) 68
PsuI RGATCY 4 cut(s) 628, 1146, 1924, 2149
PvuI CGATCG 1 cut(s) 1063
RsaI GTAC 5 cut(s) 56, 477, 619, 661, 890
RsaNI GTAC 5 cut(s) 55, 476, 618, 660, 889
RseI CAYNNNNRTG 5 cut(s) 234, 1535, 1583, 1768, 2266
SapI GCTCTTC 5 cut(s) 729, 1188, 1196, 1749, 1971
SaqAI TTAA 7 cut(s) 74, 936, 948, 1068, 1166, 1737, 1959
SatI GCNGC 8 cut(s) 64, 114, 240, 433, 718, 994, 2290, 2307
Sau3AI GATC 9 cut(s) 68, 348, 628, 1060, 1146, 1924, 2004, 2008, 2149
Sau96I GGNCC 2 cut(s) 1003, 1293
ScaI AGTACT 1 cut(s) 890
SchI GAGTC 3 cut(s) 40, 2012, 2065
SduI GDGCHC 1 cut(s) 555
SfaNI GCATC 6 cut(s) 136, 729, 777, 1168, 1575, 2196
SfcI CTRYAG 2 cut(s) 64, 777
SfuI TTCGAA 2 cut(s) 855, 979
SinI GGWCC 2 cut(s) 1003, 1293
SmiMI CAYNNNNRTG 5 cut(s) 234, 1535, 1583, 1768, 2266
SmlI CTYRAG 1 cut(s) 935
SmoI CTYRAG 1 cut(s) 935
SsiI CCGC 7 cut(s) 113, 211, 432, 489, 500, 718, 994
SspMI CTAG 3 cut(s) 317, 1844, 1928
TaaI ACNGT 6 cut(s) 340, 539, 778, 829, 1132, 1510
TaiI ACGT 3 cut(s) 166, 529, 619
TaqI TCGA 9 cut(s) 166, 327, 543, 723, 855, 979, 1581, 2007, 2277
TatI WGTACW 2 cut(s) 54, 888
TauI GCSGC 4 cut(s) 116, 435, 720, 996
TfiI GAWTC 6 cut(s) 119, 677, 1089, 1500, 1856, 1895
Tru1I TTAA 7 cut(s) 74, 936, 948, 1068, 1166, 1737, 1959
Tru9I TTAA 7 cut(s) 74, 936, 948, 1068, 1166, 1737, 1959
TscAI CASTG 3 cut(s) 1084, 1896, 2268
TseI GCWGC 4 cut(s) 63, 239, 2289, 2306
TspDTI ATGAA 9 cut(s) 10, 93, 224, 408, 740, 1350, 1380, 1439, 1928
TspGWI ACGGA 3 cut(s) 435, 456, 1721
TspRI CASTG 3 cut(s) 1084, 1896, 2268
Van91I CCANNNNNTGG 1 cut(s) 1653
Vha464I CTTAAG 1 cut(s) 935
VneI GTGCAC 1 cut(s) 551
VpaK11BI GGWCC 2 cut(s) 1003, 1293
XapI RAATTY 4 cut(s) 851, 1829, 1909, 2141
XbaI TCTAGA 2 cut(s) 316, 1843
XceI RCATGY 2 cut(s) 61, 1446
XcmI CCANNNNNNNNNTGG 1 cut(s) 385
XmiI GTMKAC 1 cut(s) 1222
XmnI GAANNNNTTC 2 cut(s) 930, 957
XspI CTAG 3 cut(s) 317, 1844, 1928
ZrmI AGTACT 1 cut(s) 890
Zsp2I ATGCAT 2 cut(s) 1254, 1590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.