RESISTANCE protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1214 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 784 |
| AbsI | CCTCGAGG | 1 cut(s) | 264 |
| AccI | GTMKAC | 1 cut(s) | 945 |
| AccII | CGCG | 1 cut(s) | 1641 |
| AciI | CCGC | 3 cut(s) | 591, 657, 1603 |
| AclWI | GGATC | 2 cut(s) | 1161, 1239 |
| AcsI | RAATTY | 5 cut(s) | 325, 609, 703, 1178, 1338 |
| AcuI | CTGAAG | 5 cut(s) | 153, 374, 434, 983, 1049 |
| AfaI | GTAC | 3 cut(s) | 529, 1143, 1688 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 1145, 1228, 1752 |
| AflII | CTTAAG | 1 cut(s) | 203 |
| AgsI | TTSAA | 8 cut(s) | 119, 178, 325, 521, 542, 601, 1453, 1856 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 235 |
| AhlI | ACTAGT | 1 cut(s) | 1514 |
| AjnI | CCWGG | 3 cut(s) | 135, 625, 1303 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 773, 805, 1791, 1823 |
| Alw26I | GTCTC | 3 cut(s) | 275, 584, 1777 |
| AlwI | GGATC | 2 cut(s) | 1161, 1239 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 904 |
| Ama87I | CYCGRG | 4 cut(s) | 41, 264, 272, 503 |
| AoxI | GGCC | 3 cut(s) | 409, 1300, 1665 |
| ApeKI | GCWGC | 8 cut(s) | 594, 659, 687, 732, 895, 1078, 1113, 1201 |
| ApoI | RAATTY | 5 cut(s) | 325, 609, 703, 1178, 1338 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 707 |
| AspS9I | GGNCC | 3 cut(s) | 139, 229, 1301 |
| AsuC2I | CCSGG | 4 cut(s) | 42, 43, 1139, 1329 |
| AsuHPI | GGTGA | 2 cut(s) | 248, 1768 |
| AvaI | CYCGRG | 4 cut(s) | 41, 264, 272, 503 |
| AvaII | GGWCC | 2 cut(s) | 139, 229 |
| BanII | GRGCYC | 1 cut(s) | 497 |
| BbsI | GAAGAC | 1 cut(s) | 272 |
| BbvI | GCAGC | 8 cut(s) | 581, 646, 699, 719, 882, 1065, 1100, 1213 |
| BccI | CCATC | 6 cut(s) | 305, 343, 1027, 1103, 1292, 1512 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 869, 903 |
| BciT130I | CCWGG | 3 cut(s) | 137, 627, 1305 |
| BclI | TGATCA | 1 cut(s) | 22 |
| BcnI | CCSGG | 4 cut(s) | 42, 43, 1139, 1329 |
| BcoDI | GTCTC | 3 cut(s) | 275, 584, 1777 |
| BcuI | ACTAGT | 1 cut(s) | 1514 |
| BfaI | CTAG | 4 cut(s) | 1124, 1242, 1290, 1515 |
| BfmI | CTRYAG | 2 cut(s) | 660, 896 |
| BfrI | CTTAAG | 1 cut(s) | 203 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 892 |
| BglII | AGATCT | 1 cut(s) | 151 |
| BisI | GCNGC | 9 cut(s) | 592, 595, 660, 688, 733, 896, 1079, 1114, 1202 |
| BlsI | GCNGC | 9 cut(s) | 593, 596, 661, 689, 734, 897, 1080, 1115, 1203 |
| Bme1390I | CCNGG | 7 cut(s) | 42, 43, 137, 627, 1139, 1305, 1329 |
| Bme18I | GGWCC | 2 cut(s) | 139, 229 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 235 |
| BmeT110I | CYCGRG | 4 cut(s) | 41, 264, 272, 503 |
| BmgT120I | GGNCC | 3 cut(s) | 139, 229, 1301 |
| BmiI | GGNNCC | 4 cut(s) | 230, 231, 457, 1839 |
| BmrFI | CCNGG | 7 cut(s) | 42, 43, 137, 627, 1139, 1305, 1329 |
| BmrI | ACTGGG | 1 cut(s) | 235 |
| BmsI | GCATC | 6 cut(s) | 174, 568, 612, 1252, 1414, 1667 |
| BmuI | ACTGGG | 1 cut(s) | 235 |
| BpiI | GAAGAC | 1 cut(s) | 272 |
| BpmI | CTGGAG | 1 cut(s) | 129 |
| BpuEI | CTTGAG | 4 cut(s) | 270, 764, 931, 1773 |
| BpuMI | CCSGG | 4 cut(s) | 42, 43, 1139, 1329 |
| Bsa29I | ATCGAT | 1 cut(s) | 444 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1382 |
| BsaI | GGTCTC | 1 cut(s) | 275 |
| BsaJI | CCNNGG | 5 cut(s) | 41, 789, 1082, 1137, 1303 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 1145, 1228, 1752 |
| Bse1I | ACTGG | 3 cut(s) | 112, 230, 849 |
| Bse3DI | GCAATG | 1 cut(s) | 848 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1382 |
| BseBI | CCWGG | 3 cut(s) | 137, 627, 1305 |
| BseCI | ATCGAT | 1 cut(s) | 444 |
| BseDI | CCNNGG | 5 cut(s) | 41, 789, 1082, 1137, 1303 |
| BseGI | GGATG | 1 cut(s) | 316 |
| BseJI | GATNNNNATC | 1 cut(s) | 1382 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 1145, 1228, 1752 |
| BseMI | GCAATG | 1 cut(s) | 848 |
| BseMII | CTCAG | 2 cut(s) | 1179, 1653 |
| BseNI | ACTGG | 3 cut(s) | 112, 230, 849 |
| BseRI | GAGGAG | 1 cut(s) | 1386 |
| BseXI | GCAGC | 8 cut(s) | 581, 646, 699, 719, 882, 1065, 1100, 1213 |
| BseYI | CCCAGC | 1 cut(s) | 890 |
| BsgI | GTGCAG | 2 cut(s) | 369, 956 |
| Bsh1236I | CGCG | 1 cut(s) | 1641 |
| BshFI | GGCC | 3 cut(s) | 411, 1302, 1667 |
| BshVI | ATCGAT | 1 cut(s) | 444 |
| BsiHKCI | CYCGRG | 4 cut(s) | 41, 264, 272, 503 |
| BsiSI | CCGG | 3 cut(s) | 42, 1139, 1328 |
| BslFI | GGGAC | 1 cut(s) | 242 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 1145, 1228, 1752 |
| BsmAI | GTCTC | 3 cut(s) | 275, 584, 1777 |
| BsmBI | CGTCTC | 1 cut(s) | 584 |
| BsmFI | GGGAC | 1 cut(s) | 242 |
| BsmI | GAATGC | 2 cut(s) | 125, 1448 |
| BsnI | GGCC | 3 cut(s) | 411, 1302, 1667 |
| Bso31I | GGTCTC | 1 cut(s) | 275 |
| BsoBI | CYCGRG | 4 cut(s) | 41, 264, 272, 503 |
| Bsp1286I | GDGCHC | 1 cut(s) | 497 |
| Bsp1407I | TGTACA | 1 cut(s) | 527 |
| Bsp19I | CCATGG | 1 cut(s) | 1082 |
| Bsp68I | TCGCGA | 1 cut(s) | 1641 |
| BspACI | CCGC | 3 cut(s) | 591, 657, 1603 |
| BspANI | GGCC | 3 cut(s) | 411, 1302, 1667 |
| BspCNI | CTCAG | 2 cut(s) | 1180, 1654 |
| BspDI | ATCGAT | 1 cut(s) | 444 |
| BspFNI | CGCG | 1 cut(s) | 1641 |
| BspHI | TCATGA | 2 cut(s) | 129, 1795 |
| BspLI | GGNNCC | 4 cut(s) | 230, 231, 457, 1839 |
| BspMAI | CTGCAG | 2 cut(s) | 664, 900 |
| BspPI | GGATC | 2 cut(s) | 1161, 1239 |
| BspTI | CTTAAG | 1 cut(s) | 203 |
| BspTNI | GGTCTC | 1 cut(s) | 275 |
| BsrDI | GCAATG | 1 cut(s) | 848 |
| BsrGI | TGTACA | 1 cut(s) | 527 |
| BsrI | ACTGG | 3 cut(s) | 112, 230, 849 |
| BssECI | CCNNGG | 5 cut(s) | 41, 789, 1082, 1137, 1303 |
| BssT1I | CCWWGG | 2 cut(s) | 789, 1082 |
| Bst2UI | CCWGG | 3 cut(s) | 137, 627, 1305 |
| Bst4CI | ACNGT | 3 cut(s) | 906, 1038, 1750 |
| BstAFI | CTTAAG | 1 cut(s) | 203 |
| BstAUI | TGTACA | 1 cut(s) | 527 |
| BstC8I | GCNNGC | 1 cut(s) | 888 |
| BstDEI | CTNAG | 7 cut(s) | 124, 194, 293, 449, 1188, 1662, 1881 |
| BstDSI | CCRYGG | 1 cut(s) | 1082 |
| BstF5I | GGATG | 1 cut(s) | 316 |
| BstFNI | CGCG | 1 cut(s) | 1641 |
| BstMAI | GTCTC | 3 cut(s) | 275, 584, 1777 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 364, 760, 892, 1012, 1084, 1198, 1664 |
| BstNI | CCWGG | 3 cut(s) | 137, 627, 1305 |
| BstNSI | RCATGY | 1 cut(s) | 1120 |
| BstSCI | CCNGG | 7 cut(s) | 40, 41, 135, 625, 1137, 1303, 1327 |
| BstSFI | CTRYAG | 2 cut(s) | 660, 896 |
| BstUI | CGCG | 1 cut(s) | 1641 |
| BstV1I | GCAGC | 8 cut(s) | 581, 646, 699, 719, 882, 1065, 1100, 1213 |
| BstV2I | GAAGAC | 1 cut(s) | 272 |
| BstX2I | RGATCY | 1 cut(s) | 151 |
| BstYI | RGATCY | 1 cut(s) | 151 |
| Bsu15I | ATCGAT | 1 cut(s) | 444 |
| BsuRI | GGCC | 3 cut(s) | 411, 1302, 1667 |
| BsuTUI | ATCGAT | 1 cut(s) | 444 |
| BtgI | CCRYGG | 1 cut(s) | 1082 |
| BtsCI | GGATG | 1 cut(s) | 316 |
| BtsI | GCAGTG | 2 cut(s) | 311, 669 |
| BtsIMutI | CAGTG | 4 cut(s) | 311, 669, 902, 1746 |
| BtuMI | TCGCGA | 1 cut(s) | 1641 |
| Cac8I | GCNNGC | 1 cut(s) | 888 |
| CaiI | CAGNNNCTG | 1 cut(s) | 904 |
| CciI | TCATGA | 2 cut(s) | 129, 1795 |
| Cfr13I | GGNCC | 3 cut(s) | 139, 229, 1301 |
| Cfr9I | CCCGGG | 1 cut(s) | 41 |
| ClaI | ATCGAT | 1 cut(s) | 444 |
| CseI | GACGC | 1 cut(s) | 232 |
| Csp6I | GTAC | 3 cut(s) | 528, 1142, 1687 |
| CviQI | GTAC | 3 cut(s) | 528, 1142, 1687 |
| DdeI | CTNAG | 7 cut(s) | 124, 194, 293, 449, 1188, 1662, 1881 |
| DraI | TTTAAA | 1 cut(s) | 1174 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 784 |
| DriI | GACNNNNNGTC | 1 cut(s) | 235 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 784 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 235 |
| EciI | GGCGGA | 1 cut(s) | 1592 |
| Eco130I | CCWWGG | 2 cut(s) | 789, 1082 |
| Eco147I | AGGCCT | 2 cut(s) | 411, 1667 |
| Eco24I | GRGCYC | 1 cut(s) | 497 |
| Eco31I | GGTCTC | 1 cut(s) | 275 |
| Eco32I | GATATC | 1 cut(s) | 1867 |
| Eco47I | GGWCC | 2 cut(s) | 139, 229 |
| Eco57I | CTGAAG | 5 cut(s) | 153, 374, 434, 983, 1049 |
| Eco88I | CYCGRG | 4 cut(s) | 41, 264, 272, 503 |
| EcoO109I | RGGNCCY | 1 cut(s) | 229 |
| EcoRI | GAATTC | 2 cut(s) | 325, 1178 |
| EcoRII | CCWGG | 3 cut(s) | 135, 625, 1303 |
| EcoRV | GATATC | 1 cut(s) | 1867 |
| EcoT14I | CCWWGG | 2 cut(s) | 789, 1082 |
| EcoT22I | ATGCAT | 2 cut(s) | 189, 1439 |
| EcoT38I | GRGCYC | 1 cut(s) | 497 |
| ErhI | CCWWGG | 2 cut(s) | 789, 1082 |
| Esp3I | CGTCTC | 1 cut(s) | 584 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 243, 275, 676, 708 |
| FaqI | GGGAC | 1 cut(s) | 242 |
| FbaI | TGATCA | 1 cut(s) | 22 |
| FblI | GTMKAC | 1 cut(s) | 945 |
| Fnu4HI | GCNGC | 9 cut(s) | 592, 595, 660, 688, 733, 896, 1079, 1114, 1202 |
| FokI | GGATG | 1 cut(s) | 323 |
| FriOI | GRGCYC | 1 cut(s) | 497 |
| Fsp4HI | GCNGC | 9 cut(s) | 592, 595, 660, 688, 733, 896, 1079, 1114, 1202 |
| FspBI | CTAG | 4 cut(s) | 1124, 1242, 1290, 1515 |
| GluI | GCNGC | 9 cut(s) | 592, 595, 660, 688, 733, 896, 1079, 1114, 1202 |
| GsaI | CCCAGC | 1 cut(s) | 894 |
| GsuI | CTGGAG | 1 cut(s) | 129 |
| HaeIII | GGCC | 3 cut(s) | 411, 1302, 1667 |
| HapII | CCGG | 3 cut(s) | 42, 1139, 1328 |
| HgaI | GACGC | 1 cut(s) | 232 |
| HincII | GTYRAC | 3 cut(s) | 247, 946, 1165 |
| HindII | GTYRAC | 3 cut(s) | 247, 946, 1165 |
| HindIII | AAGCTT | 2 cut(s) | 356, 1388 |
| HinfI | GANTC | 7 cut(s) | 37, 866, 925, 991, 1057, 1275, 1770 |
| HpaII | CCGG | 3 cut(s) | 42, 1139, 1328 |
| HphI | GGTGA | 2 cut(s) | 248, 1768 |
| Hpy166II | GTNNAC | 5 cut(s) | 247, 256, 946, 1165, 1746 |
| Hpy188I | TCNGA | 6 cut(s) | 151, 486, 713, 1236, 1579, 1663 |
| Hpy8I | GTNNAC | 5 cut(s) | 247, 256, 946, 1165, 1746 |
| HpyCH4III | ACNGT | 3 cut(s) | 906, 1038, 1750 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 364, 760, 892, 1012, 1084, 1198, 1664 |
| HpyF3I | CTNAG | 7 cut(s) | 124, 194, 293, 449, 1188, 1662, 1881 |
| KflI | GGGWCCC | 1 cut(s) | 229 |
| Ksp22I | TGATCA | 1 cut(s) | 22 |
| LmnI | GCTCC | 4 cut(s) | 110, 429, 461, 1245 |
| Lsp1109I | GCAGC | 8 cut(s) | 581, 646, 699, 719, 882, 1065, 1100, 1213 |
| LweI | GCATC | 6 cut(s) | 174, 568, 612, 1252, 1414, 1667 |
| MaeI | CTAG | 4 cut(s) | 1124, 1242, 1290, 1515 |
| MaeIII | GTNAC | 4 cut(s) | 207, 221, 1119, 1424 |
| MboII | GAAGA | 4 cut(s) | 272, 427, 700, 1874 |
| MfeI | CAATTG | 2 cut(s) | 114, 916 |
| MflI | RGATCY | 1 cut(s) | 151 |
| MhlI | GDGCHC | 1 cut(s) | 497 |
| MlyI | GAGTC | 6 cut(s) | 875, 934, 1000, 1066, 1284, 1779 |
| Mph1103I | ATGCAT | 2 cut(s) | 189, 1439 |
| MroXI | GAANNNNTTC | 1 cut(s) | 707 |
| MseI | TTAA | 4 cut(s) | 33, 204, 434, 1173 |
| MspA1I | CMGCKG | 1 cut(s) | 659 |
| MspCI | CTTAAG | 1 cut(s) | 203 |
| MspI | CCGG | 3 cut(s) | 42, 1139, 1328 |
| MspR9I | CCNGG | 7 cut(s) | 42, 43, 137, 627, 1139, 1305, 1329 |
| MunI | CAATTG | 2 cut(s) | 114, 916 |
| Mva1269I | GAATGC | 2 cut(s) | 125, 1448 |
| MvaI | CCWGG | 3 cut(s) | 137, 627, 1305 |
| MvnI | CGCG | 1 cut(s) | 1641 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 364, 760, 892, 1012, 1084, 1198, 1664 |
| NciI | CCSGG | 4 cut(s) | 42, 43, 1139, 1329 |
| NcoI | CCATGG | 1 cut(s) | 1082 |
| NlaIV | GGNNCC | 4 cut(s) | 230, 231, 457, 1839 |
| NmeAIII | GCCGAG | 1 cut(s) | 811 |
| NmuCI | GTSAC | 1 cut(s) | 1119 |
| NruI | TCGCGA | 1 cut(s) | 1641 |
| NsiI | ATGCAT | 2 cut(s) | 189, 1439 |
| NspI | RCATGY | 1 cut(s) | 1120 |
| PaeR7I | CTCGAG | 3 cut(s) | 264, 272, 503 |
| PagI | TCATGA | 2 cut(s) | 129, 1795 |
| PceI | AGGCCT | 2 cut(s) | 411, 1667 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1279 |
| PctI | GAATGC | 2 cut(s) | 125, 1448 |
| PdmI | GAANNNNTTC | 1 cut(s) | 707 |
| PfeI | GAWTC | 1 cut(s) | 37 |
| PfoI | TCCNGGA | 1 cut(s) | 1327 |
| PkrI | GCNGC | 9 cut(s) | 593, 596, 661, 689, 734, 897, 1080, 1115, 1203 |
| PleI | GAGTC | 6 cut(s) | 874, 933, 999, 1065, 1283, 1778 |
| PpsI | GAGTC | 6 cut(s) | 874, 933, 999, 1065, 1283, 1778 |
| PpuMI | RGGWCCY | 1 cut(s) | 229 |
| PsiI | TTATAA | 1 cut(s) | 1214 |
| Psp5II | RGGWCCY | 1 cut(s) | 229 |
| Psp6I | CCWGG | 3 cut(s) | 135, 625, 1303 |
| PspFI | CCCAGC | 1 cut(s) | 890 |
| PspGI | CCWGG | 3 cut(s) | 135, 625, 1303 |
| PspN4I | GGNNCC | 4 cut(s) | 230, 231, 457, 1839 |
| PspPI | GGNCC | 3 cut(s) | 139, 229, 1301 |
| PspPPI | RGGWCCY | 1 cut(s) | 229 |
| PspXI | VCTCGAGB | 1 cut(s) | 264 |
| PstI | CTGCAG | 2 cut(s) | 664, 900 |
| PstNI | CAGNNNCTG | 1 cut(s) | 904 |
| PsuI | RGATCY | 1 cut(s) | 151 |
| RruI | TCGCGA | 1 cut(s) | 1641 |
| RsaI | GTAC | 3 cut(s) | 529, 1143, 1688 |
| RsaNI | GTAC | 3 cut(s) | 528, 1142, 1687 |
| SalI | GTCGAC | 1 cut(s) | 944 |
| SaqAI | TTAA | 4 cut(s) | 33, 204, 434, 1173 |
| SatI | GCNGC | 9 cut(s) | 592, 595, 660, 688, 733, 896, 1079, 1114, 1202 |
| Sau96I | GGNCC | 3 cut(s) | 139, 229, 1301 |
| SchI | GAGTC | 6 cut(s) | 875, 934, 1000, 1066, 1284, 1779 |
| ScrFI | CCNGG | 7 cut(s) | 42, 43, 137, 627, 1139, 1305, 1329 |
| SduI | GDGCHC | 1 cut(s) | 497 |
| SfaNI | GCATC | 6 cut(s) | 174, 568, 612, 1252, 1414, 1667 |
| SfcI | CTRYAG | 2 cut(s) | 660, 896 |
| Sfr274I | CTCGAG | 3 cut(s) | 264, 272, 503 |
| SinI | GGWCC | 2 cut(s) | 139, 229 |
| SlaI | CTCGAG | 3 cut(s) | 264, 272, 503 |
| SmaI | CCCGGG | 1 cut(s) | 43 |
| SmlI | CTYRAG | 8 cut(s) | 203, 264, 272, 285, 503, 743, 910, 1788 |
| SmoI | CTYRAG | 8 cut(s) | 203, 264, 272, 285, 503, 743, 910, 1788 |
| SpeI | ACTAGT | 1 cut(s) | 1514 |
| SseBI | AGGCCT | 2 cut(s) | 411, 1667 |
| SsiI | CCGC | 3 cut(s) | 591, 657, 1603 |
| SspMI | CTAG | 4 cut(s) | 1124, 1242, 1290, 1515 |
| StuI | AGGCCT | 2 cut(s) | 411, 1667 |
| StyD4I | CCNGG | 7 cut(s) | 40, 41, 135, 625, 1137, 1303, 1327 |
| StyI | CCWWGG | 2 cut(s) | 789, 1082 |
| TaaI | ACNGT | 3 cut(s) | 906, 1038, 1750 |
| TatI | WGTACW | 1 cut(s) | 527 |
| TauI | GCSGC | 1 cut(s) | 594 |
| TfiI | GAWTC | 1 cut(s) | 37 |
| Tru1I | TTAA | 4 cut(s) | 33, 204, 434, 1173 |
| Tru9I | TTAA | 4 cut(s) | 33, 204, 434, 1173 |
| TscAI | CASTG | 4 cut(s) | 311, 669, 909, 1753 |
| TseFI | GTSAC | 1 cut(s) | 1119 |
| TseI | GCWGC | 8 cut(s) | 594, 659, 687, 732, 895, 1078, 1113, 1201 |
| Tsp45I | GTSAC | 1 cut(s) | 1119 |
| TspDTI | ATGAA | 4 cut(s) | 707, 1408, 1669, 1812 |
| TspMI | CCCGGG | 1 cut(s) | 41 |
| TspRI | CASTG | 4 cut(s) | 311, 669, 909, 1753 |
| Vha464I | CTTAAG | 1 cut(s) | 203 |
| VpaK11BI | GGWCC | 2 cut(s) | 139, 229 |
| XapI | RAATTY | 5 cut(s) | 325, 609, 703, 1178, 1338 |
| XceI | RCATGY | 1 cut(s) | 1120 |
| XhoI | CTCGAG | 3 cut(s) | 264, 272, 503 |
| XmaI | CCCGGG | 1 cut(s) | 41 |
| XmiI | GTMKAC | 1 cut(s) | 945 |
| XmnI | GAANNNNTTC | 1 cut(s) | 707 |
| XspI | CTAG | 4 cut(s) | 1124, 1242, 1290, 1515 |
| Zsp2I | ATGCAT | 2 cut(s) | 189, 1439 |